Consider the following premises. forall x_5: ~R_5(x_5). R_5(a_5) <-> q. Can we infer ~q from them?
 From forall x_5: ~R_5(x_5) we can infer ~R_5(a_5). Finally, from R_5(a_5) <-> q, ~R_5(a_5) we can infer ~q. Therefore, the answer is yes.