Consider the following premises. s_1. R_16(j) <-> t_2. forall x_16: ~R_16(x_16). Can we infer t_2 from them? If possible, name the inference rules being used at each step.
 From forall x_16: ~R_16(x_16) we can infer ~R_16(j) via universal instantiation. Finally, from R_16(j) <-> t_2, ~R_16(j) we can infer ~t_2 via biconditional elimination, which contradicts t_2. Therefore, the answer is no.