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The phi X 174 (or ÏX174) bacteriophage was the first DNA-based genome to be sequenced.This work was completed by Fred Sanger and his team in 1977. In 1962, Walter Fiers and Robert Sinsheimer had already demonstrated the physical, covalently closed circularity of ÏX174 DNA.n 2003, it was reported by Craig Venter's group that the genome of ÏX174 was the first to be completely assembled in vitro from synthesized oligonucleotides. The ÏX174 virus particle has also been successfully assembled in vitro.
Bacteriophage f1 is structurally classified as a class I filamentous phage, and is closely related to the other Ff phages, such as M13 and phage fd. In the following article, genes will be written in italics and their associated proteins in Roman.he presence of a properly presented ssDNA in an adhesion zone with thioredoxin and the coat proteins allows for the elongation and secretion of phages. The phage is elongated by continually removing dimers of V and replacing them with the major coat protein. The phage coat consists of about 2700 copies of VIII.