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The phi X 174 (or ÏX174) bacteriophage was the first DNA-based genome to be sequenced.This work was completed by Fred Sanger and his team in 1977. In 1962, Walter Fiers and Robert Sinsheimer had already demonstrated the physical, covalently closed circularity of ÏX174 DNA.n 2003, it was reported by Craig Venter's group that the genome of ÏX174 was the first to be completely assembled in vitro from synthesized oligonucleotides. The ÏX174 virus particle has also been successfully assembled in vitro.
Enterobacteria phage T4 is a bacteriophage that infects Escherichia coli bacteria. The T4 phage is a member of the T-even phages, a group including enterobacteriophages T2 and T6. (Chapter 3 provides overview of various T4-like phages as well as the isolation of then-new T4-like phages). 2  Surdis, T.J et al Bacteriophage attachment methods specific to T4, analysis, Overview. 3  Mathews, C. K., E. M. Kutter, G. Mosig, and P. B. Berget. 1983.