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No. The A1C test measures the amount of glucose bound to hemoglobin in the red blood cells. This binding process is related to the concentration of glucose present and the time of exposure. New cells are constantly created to replace old cells that are removed.
For example, you will not need to fast prior to a pregnancy blood test. Fasting is also not required for many routine tests of blood chemistry -- for example, tests that measure kidney, liver or thyroid function can be completed any time of the day. Even the test that measures a 2 to 3 month average blood glucose -- the A1c test -- does not require fasting.