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Tryptase (EC 3.4.21.59, ) is the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as a marker for mast cell activation.
Tryptophan is a very commonly used intrinsic probe for determining the conformational state of a protein. The excitation wavelength of the indole group of tryptophan is commonly ~295 nm and the emission is between 300 and 350 nm.