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The simplest biochemical test that is used to differentiate between Streptococcus and Staphylococcus species is the Catalase test. In this test we are testing for the ability of the bacteria to produce the enzyme catalase.
The coagulase test has traditionally been used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).t is also produced by Yersinia pestis. Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen, a plasma protein produced by the liver, to fibrin.