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How does a gram stain differentiate between bacteria? It differentiates between bacteria based on the structure of their cell walls. Why do bacteria respond differently to a Gram stain? - It responds differently because of the thickness of peptidoglycan in the cell walls - Stains fix differently based on cell wall structure
It is believed that the gram staining characteristics of an organism is a function of its cell wall. Therefore any time the cell wall is damaged the gram stain characteristics of gram positive cells will change. On the other hand, gram (-) cells never become gram (+) if the stain procedure is done correctly. There are fundamental biological differences between gram (+) and gram (-) cells. These are summarized in Table 4.1(Tortora et. al.) and I'd like to stress the following points: