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The simplest biochemical test that is used to differentiate between Streptococcus and Staphylococcus species is the Catalase test. In this test we are testing for the ability of the bacteria to produce the enzyme catalase.
Catalase Test. The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme, catalase, is produced by bacteria that respire using oxygen, and protects them from the toxic by-products of oxygen metabolism. Catalase-positive bacteria include strict aerobes as well as facultative anaerobes, although they all have the ability to respire using oxygen as a terminal electron acceptor.