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How does a gram stain differentiate between bacteria? It differentiates between bacteria based on the structure of their cell walls. Why do bacteria respond differently to a Gram stain? - It responds differently because of the thickness of peptidoglycan in the cell walls - Stains fix differently based on cell wall structure
By following the Gram stain procedure I will determine which type(s) of bacteria are in the cultures grown in the science lab. Background Information: Differential stains are very useful in microbiology because they can be used to distinguish between groups of bacteria. The Gram stain is one of the most important steps in the characterization and identification of bacteria. The Gram stain separates bacteria into one of two groups: 1) Gram-positive bacteria. These retain the color of the first stain used (Crystal Violet).