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On the other hand, chromatin is a mass of genetic material containing DNA, RNA, â¦ and various proteins that forms chromosomes. **before mitosis, the DNA is loose in the nucleus in the form of chromatin. When the DNA begins to undergo mitosis the chromatin condenses to form the chromosome. The difference between chromatin and chromosomes is that  chromosomes help to make up chromatin. Chromatin is made up of DNA  and proteins, and is what makes the nucleus of a cell. Chromosomes  make up the DNA.
Chromosomes are made up of a DNA-protein complex called chromatin that is organized into subunits called nucleosomes. The way in which eukaryotes compact and arrange their chromatin not only allows a large amount of DNA to fit in a small space, but it also helps regulate gene expression.