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The phi X 174 (or ÏX174) bacteriophage was the first DNA-based genome to be sequenced.This work was completed by Fred Sanger and his team in 1977. In 1962, Walter Fiers and Robert Sinsheimer had already demonstrated the physical, covalently closed circularity of ÏX174 DNA.n 2003, it was reported by Craig Venter's group that the genome of ÏX174 was the first to be completely assembled in vitro from synthesized oligonucleotides. The ÏX174 virus particle has also been successfully assembled in vitro.
In particular, the bacteriophage T4 is a virus which infects E.Coli, a bacteria that has been used extensively for molecular biology research. The bacteriophage T4 exemplifies the life cycle of viruses. It exists as an inactive virion until one of its extended 'legs' comes into contact with the surface of an E. Coli.n nature, the bacteriophage T4 contains about 168,800 base pairs of double stranded DNA. This genetic blueprint contains all of the necessary information to create new bacteriophage T4. Our virtual bacteriophage T4 also contains its own genetic information; it is made up of characters of ascii VRML 2.0 code.