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In iron deficiency, the iron level is low but the TIBC is increased, thus transferrin saturation becomes very low. In iron overload states, such as hemochromatosis, the iron level will be high and the TIBC will be low or normal, causing the transferrin saturation to increase. UIBC may be ordered as an alternative to TIBC.
It is normal for the transferrin in the blood to have 3-5 carbohydrate side-chains, however people who misuse alcohol have a higher proportion of transferrin in their blood which only have 0-2 carbohydrate side-chains. Hence the term carbohydrate deficient transferrin.