Chemical chaperones reverse early suppression of regulatory circuits during Unfolded Protein Response in B cells from Common Variable Immunodeficiency patients
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'Rare B cell samples from two CVID patients (P1 and P2) and a healthy donor (C) were used in controlled experiments analyzing the early UPR with and without the presence of chemical chaperones. As ER stressors we used Thapsigargin (Tg) and Tunicamycin (Tm). As exogenous chaperones we used DMSO, PBA, and TUDCA. For quantification of specific transcripts of interest we used a high-throughput platform based on TaqMan technology (Applied Biosystems TaqMan OpenArray Real-Time PCR System). Samples containing 3 x 10e5 EBV-B cells that received treatments with ER stressors and/or chemical chaperones for 0, 1, 2, 3, or 4 hr. Purified RNA was quali- and quantified by spectrometry in a Nanodrop and by fluorimetry in a Qubit, respectively. cDNA was generated using 300 ng of total RNA, and amplified in triplicate reactions for quantification of speci' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["B cell","Common Variable Immunodeficiency","gene expression","Gene Regulatory Network","human","Unfolded Protein Response"]} <|eot_id|>
 Here is the Description of a dataset. Description:'Rare B cell samples from two CVID patients (P1 and P2) and a healthy donor (C) were used in controlled experiments analyzing the early UPR with and without the presence of chemical chaperones. As ER stressors we used Thapsigargin (Tg) and Tunicamycin (Tm). As exogenous chaperones we used DMSO, PBA, and TUDCA. For quantification of specific transcripts of interest we used a high-throughput platform based on TaqMan technology (Applied Biosystems TaqMan OpenArray Real-Time PCR System). Samples containing 3 x 10e5 EBV-B cells that received treatments with ER stressors and/or chemical chaperones for 0, 1, 2, 3, or 4 hr. Purified RNA was quali- and quantified by spectrometry in a Nanodrop and by fluorimetry in a Qubit, respectively. cDNA was generated using 300 ng of total RNA, and amplified in triplicate reactions for quantification of speci' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
Rare B cell samples from two CVID patients (P1 and P2) and a healthy donor (C) were used in controlled experiments analyzing the early UPR with and without the presence of chemical chaperones. As ER stressors we used Thapsigargin (Tg) and Tunicamycin (Tm). As exogenous chaperones we used DMSO, PBA, and TUDCA. For quantification of specific transcripts of interest we used a high-throughput platform based on TaqMan technology (Applied Biosystems TaqMan OpenArray Real-Time PCR System). Samples containing 3 x 10e5 EBV-B cells that received treatments with ER stressors and/or chemical chaperones for 0, 1, 2, 3, or 4 hr. Purified RNA was quali- and quantified by spectrometry in a Nanodrop and by fluorimetry in a Qubit, respectively. cDNA was generated using 300 ng of total RNA, and amplified in triplicate reactions for quantification of speci
B cell; Common Variable Immunodeficiency; gene expression; Gene Regulatory Network; human; Unfolded Protein Response