The phospholipase effector Tle1Vc promotes Vibrio cholerae virulence by killing competitors and impacting gene expression
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'Vibrio cholerae utilizes the Type VI secretion system (T6SS) to gain an advantage in interbacterial competition by delivering antiprokaryotic effectors in a contact-dependent manner. However, the impact of T6SS and its secreted effectors on physiological behavior remains poorly understood. In this study, we present Tle1Vc, a lipase effector in atypical pathogenic V. cholerae E1 that is secreted by T6SS via its interaction with VgrG1E1. Tle1Vc contains a DUF2235 domain and belongs to the Tle1 (type VI lipase effector) family. Bacterial toxicity assays, lipase activity assays, and site-directed mutagenesis revealed that Tle1Vc-induced toxicity required two aspartic acid residues (D417 and D496) and a serine residue (S356). Cell intoxication with Tle1Vc leads to membrane depolarization and altered membrane permeability. Tli1tox-, a cognate' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["Vibrio cholerae","T6SS","phospholipase effector","tit-for-tat","motility"]} <|eot_id|>
 Here is the Description of a dataset. Description:'Vibrio cholerae utilizes the Type VI secretion system (T6SS) to gain an advantage in interbacterial competition by delivering antiprokaryotic effectors in a contact-dependent manner. However, the impact of T6SS and its secreted effectors on physiological behavior remains poorly understood. In this study, we present Tle1Vc, a lipase effector in atypical pathogenic V. cholerae E1 that is secreted by T6SS via its interaction with VgrG1E1. Tle1Vc contains a DUF2235 domain and belongs to the Tle1 (type VI lipase effector) family. Bacterial toxicity assays, lipase activity assays, and site-directed mutagenesis revealed that Tle1Vc-induced toxicity required two aspartic acid residues (D417 and D496) and a serine residue (S356). Cell intoxication with Tle1Vc leads to membrane depolarization and altered membrane permeability. Tli1tox-, a cognate' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
Vibrio cholerae utilizes the Type VI secretion system (T6SS) to gain an advantage in interbacterial competition by delivering antiprokaryotic effectors in a contact-dependent manner. However, the impact of T6SS and its secreted effectors on physiological behavior remains poorly understood. In this study, we present Tle1Vc, a lipase effector in atypical pathogenic V. cholerae E1 that is secreted by T6SS via its interaction with VgrG1E1. Tle1Vc contains a DUF2235 domain and belongs to the Tle1 (type VI lipase effector) family. Bacterial toxicity assays, lipase activity assays, and site-directed mutagenesis revealed that Tle1Vc-induced toxicity required two aspartic acid residues (D417 and D496) and a serine residue (S356). Cell intoxication with Tle1Vc leads to membrane depolarization and altered membrane permeability. Tli1tox-, a cognate
Vibrio cholerae; T6SS; phospholipase effector; tit-for-tat; motility