Replication Data for: Optimizing a three-dimensional spheroid clearing method for the imaging-based evaluation of cardiotoxicity
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'BackgroundToxicity evaluation based on two-dimensional cell culture shows differences from clinical results and has the disadvantage of not accurately reflecting cell-to-cell cross-signaling. Since almost all cells in the human body are arranged in a three-dimensional structure and constitute a tissue, the in vitro reproduction of a three-dimensional tissue composed of human cells can be used as an effective model for drug development and toxicity evaluation. The clearing technique improves image resolution and can implement three-dimensional bio-images throughout the organization, enabling more efficient toxicity evaluation of disease model analysis using spheroids.MethodsWe generate an 100-200um spheroids and optimized clearing condition. Next application and imaging of optimized clearing condition in the cardiac spheroid model.Re' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["Clearing","3D cell culture","Spheroid","Predictive toxicology"]} <|eot_id|>
 Here is the Description of a dataset. Description:'BackgroundToxicity evaluation based on two-dimensional cell culture shows differences from clinical results and has the disadvantage of not accurately reflecting cell-to-cell cross-signaling. Since almost all cells in the human body are arranged in a three-dimensional structure and constitute a tissue, the in vitro reproduction of a three-dimensional tissue composed of human cells can be used as an effective model for drug development and toxicity evaluation. The clearing technique improves image resolution and can implement three-dimensional bio-images throughout the organization, enabling more efficient toxicity evaluation of disease model analysis using spheroids.MethodsWe generate an 100-200um spheroids and optimized clearing condition. Next application and imaging of optimized clearing condition in the cardiac spheroid model.Re' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
BackgroundToxicity evaluation based on two-dimensional cell culture shows differences from clinical results and has the disadvantage of not accurately reflecting cell-to-cell cross-signaling. Since almost all cells in the human body are arranged in a three-dimensional structure and constitute a tissue, the in vitro reproduction of a three-dimensional tissue composed of human cells can be used as an effective model for drug development and toxicity evaluation. The clearing technique improves image resolution and can implement three-dimensional bio-images throughout the organization, enabling more efficient toxicity evaluation of disease model analysis using spheroids.MethodsWe generate an 100-200um spheroids and optimized clearing condition. Next application and imaging of optimized clearing condition in the cardiac spheroid model.Re
Clearing; 3D cell culture; Spheroid; Predictive toxicology