Cloning of Human ABCB11 Gene in E. coli required the removal of an Intragenic Pribnow-Schaller Box before it’s Insertion into Genomic Safe Harbor AAVS1 Site using CRISPR Cas9
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'Sequencing data and original images including some of the failed experiments can be found here. Context: Please refer the manuscript.Sequencing data:1) Cloning of ABCB11--sequencing2) Cloning of gRNA to two Cas9 vectors3) Sequencing for ABCB11 integration to HEK293T  cell line genome at AAVS1 site4) Sequencing for off target sites-AAVS1 site HEK293T cell genomic DNAPAGE and Western Blot Data:1) E. Coli lysate transformed ad untransformed DH5a PAGE and W/B with anti ABCB11 antibody2)  ABCB11 transfected cell lines--whole protein fibroblasts3) ABCB11 integration mediated by Cas9 cell line-whole protein fibroblasts4)  Puromycin selection cellines HEKAgaraose gel images: T7 assay and PCRsComet Assa' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["ABCB11,  Pribnow-Schaller Box,  AAVS1 Site,CRISPR Cas9"]} <|eot_id|>
 Here is the Description of a dataset. Description:'Sequencing data and original images including some of the failed experiments can be found here. Context: Please refer the manuscript.Sequencing data:1) Cloning of ABCB11--sequencing2) Cloning of gRNA to two Cas9 vectors3) Sequencing for ABCB11 integration to HEK293T  cell line genome at AAVS1 site4) Sequencing for off target sites-AAVS1 site HEK293T cell genomic DNAPAGE and Western Blot Data:1) E. Coli lysate transformed ad untransformed DH5a PAGE and W/B with anti ABCB11 antibody2)  ABCB11 transfected cell lines--whole protein fibroblasts3) ABCB11 integration mediated by Cas9 cell line-whole protein fibroblasts4)  Puromycin selection cellines HEKAgaraose gel images: T7 assay and PCRsComet Assa' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
Sequencing data and original images including some of the failed experiments can be found here. Context: Please refer the manuscript.Sequencing data:1) Cloning of ABCB11--sequencing2) Cloning of gRNA to two Cas9 vectors3) Sequencing for ABCB11 integration to HEK293T  cell line genome at AAVS1 site4) Sequencing for off target sites-AAVS1 site HEK293T cell genomic DNAPAGE and Western Blot Data:1) E. Coli lysate transformed ad untransformed DH5a PAGE and W/B with anti ABCB11 antibody2)  ABCB11 transfected cell lines--whole protein fibroblasts3) ABCB11 integration mediated by Cas9 cell line-whole protein fibroblasts4)  Puromycin selection cellines HEKAgaraose gel images: T7 assay and PCRsComet Assa
ABCB11,  Pribnow-Schaller Box,  AAVS1 Site,CRISPR Cas9