SNPs Mutation of Tropoelastin Gene Affects Tropoelastin mRNA and Elastin Expressions in Human Aortic Smooth Muscle Cells
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'Objectives: We aimed to explore the effects of single nucleotide polymorphisms (SNPs) in tropoelastin gene on tropoelastin mRNA and elastin expressions in human aortic smooth muscle cells (HASMCs).Methods: Two SNP loci, rs2071307(G/A) and rs1785598(G/C), were selected to construct recombinant lentivirus vector carrying wild-type and mutant tropoelastin gene. Recombinant plasmids, pWSLV-02-ELN, pWSLV-02-ELN-mut1 and pWSLV-02-ELN-mut2, were constructed, prior to amplified by polymerase chain reaction (PCR) and sequenced. The prepared plasmids and the packaging plasmids (pVSV-G and psPAX2) were co-transfected into HEK293T cells, to obtain recombinant lentivirus carrying tropoelastin gene. Afterwards, HASMCs were infected with recombinant lentivirus and the positive cells sorted by flow cytometry were amplified. Four stable HASMC cell lines' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["aortic dissection","elastin","human aortic smooth muscle cells","lentivirus vector","single nucleotide polymorphisms","tropoelastin gene"]} <|eot_id|>
 Here is the Description of a dataset. Description:'Objectives: We aimed to explore the effects of single nucleotide polymorphisms (SNPs) in tropoelastin gene on tropoelastin mRNA and elastin expressions in human aortic smooth muscle cells (HASMCs).Methods: Two SNP loci, rs2071307(G/A) and rs1785598(G/C), were selected to construct recombinant lentivirus vector carrying wild-type and mutant tropoelastin gene. Recombinant plasmids, pWSLV-02-ELN, pWSLV-02-ELN-mut1 and pWSLV-02-ELN-mut2, were constructed, prior to amplified by polymerase chain reaction (PCR) and sequenced. The prepared plasmids and the packaging plasmids (pVSV-G and psPAX2) were co-transfected into HEK293T cells, to obtain recombinant lentivirus carrying tropoelastin gene. Afterwards, HASMCs were infected with recombinant lentivirus and the positive cells sorted by flow cytometry were amplified. Four stable HASMC cell lines' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
Objectives: We aimed to explore the effects of single nucleotide polymorphisms (SNPs) in tropoelastin gene on tropoelastin mRNA and elastin expressions in human aortic smooth muscle cells (HASMCs).Methods: Two SNP loci, rs2071307(G/A) and rs1785598(G/C), were selected to construct recombinant lentivirus vector carrying wild-type and mutant tropoelastin gene. Recombinant plasmids, pWSLV-02-ELN, pWSLV-02-ELN-mut1 and pWSLV-02-ELN-mut2, were constructed, prior to amplified by polymerase chain reaction (PCR) and sequenced. The prepared plasmids and the packaging plasmids (pVSV-G and psPAX2) were co-transfected into HEK293T cells, to obtain recombinant lentivirus carrying tropoelastin gene. Afterwards, HASMCs were infected with recombinant lentivirus and the positive cells sorted by flow cytometry were amplified. Four stable HASMC cell lines
aortic dissection; elastin; human aortic smooth muscle cells; lentivirus vector; single nucleotide polymorphisms; tropoelastin gene