Replication Data for: Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'This dataset comprises of Nucleotide sequences used to match primers and probes, and for phylogenetic inferences.  A total of 168 N and G gene sequences deposited in Genbank with accession numbers from KX775772 - KX775940. This data is freely available. In our analysis, we observed inconsistencies between two routine RSV diagnostic methods applied in our labs, one method utilizes immunofluorescence microscopy and other molecular probes, and set out to determine.Sequence data cleaning and contig assembly was done using Sequencher program v.5.1; sequence alignment done using MAFFT v.1.8; phylogenetic inferences done in MEGA v1.6; custom BLAST of the primers and probes done using geneious v8.1.8 </p' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["Probe","Real-Time Mismatches","Respiratory Syncytial Virus"]} <|eot_id|>
 Here is the Description of a dataset. Description:'This dataset comprises of Nucleotide sequences used to match primers and probes, and for phylogenetic inferences.  A total of 168 N and G gene sequences deposited in Genbank with accession numbers from KX775772 - KX775940. This data is freely available. In our analysis, we observed inconsistencies between two routine RSV diagnostic methods applied in our labs, one method utilizes immunofluorescence microscopy and other molecular probes, and set out to determine.Sequence data cleaning and contig assembly was done using Sequencher program v.5.1; sequence alignment done using MAFFT v.1.8; phylogenetic inferences done in MEGA v1.6; custom BLAST of the primers and probes done using geneious v8.1.8 </p' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
This dataset comprises of Nucleotide sequences used to match primers and probes, and for phylogenetic inferences.  A total of 168 N and G gene sequences deposited in Genbank with accession numbers from KX775772 - KX775940. This data is freely available. In our analysis, we observed inconsistencies between two routine RSV diagnostic methods applied in our labs, one method utilizes immunofluorescence microscopy and other molecular probes, and set out to determine.Sequence data cleaning and contig assembly was done using Sequencher program v.5.1; sequence alignment done using MAFFT v.1.8; phylogenetic inferences done in MEGA v1.6; custom BLAST of the primers and probes done using geneious v8.1.8 </p
Probe; Real-Time Mismatches; Respiratory Syncytial Virus