You have to be wearing the item for the bonus to be effective; if you take it off obviously the bonus goes away. The difference being that in the first 24 hours it is a "temporary ability bonus" and afterwards it is the standard "enhancement bonus". Presumably there's something somewhere that treats the two differently, although in practice it probably won't come up. Speaking of practicality, technically if you take the item off for example to go to sleep or to have sexy times, the 24 hour countdown starts all over again... although personally I'd rule against that and just let the enhancement bonus continue.<br/><br/>Also, congrats on beating the first encounter of the dungeon with flying colors. Thanks for proving that I don't have to hold back on the devious encounters from here on.  >:)