Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability
Yewande Dosunmu, Richard Owusu-Apenten
Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate(MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS).Study Design: In-vitro method.Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2',7'-dichlorofluroscein diacetate assay.Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l).Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1
Journal of Applied Life Sciences International
https://doi.org/10.9734/JALSI/2017/37185
dehydroascorbic acid
MDA-MB-231 cells
Reactive oxygen species. Intracellular oxidative stress
10.9734/JALSI/2017/37185
Apenten1422017JALSI37185Final published version, 1 MB
http://uir.ulster.ac.uk/38933/1/20171025-AcceptanceLetteer_%20JIAL_37185.pdf
Dive into the research topics of 'Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability'. Together they form a unique fingerprint.
dehydroascorbic acid Agriculture & Biology 100%
methotrexate Agriculture & Biology 89%
breast neoplasms Agriculture & Biology 78%
cell viability Agriculture & Biology 63%
ascorbic acid Agriculture & Biology 55%
reactive oxygen species Agriculture & Biology 28%
synergism Agriculture & Biology 25%
Dosunmu, Y., & Owusu-Apenten, R. (2017). Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability. Journal of Applied Life Sciences International, 14(2). https://doi.org/10.9734/JALSI/2017/37185
Dosunmu, Yewande ; Owusu-Apenten, Richard. / Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability. In: Journal of Applied Life Sciences International. 2017 ; Vol. 14, No. 2.
@article{7935e7345fea4c2a8174205bf184b456,
title = "Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability",
abstract = "Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate(MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS).Study Design: In-vitro method.Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2{\textquoteright},7{\textquoteright}-dichlorofluroscein diacetate assay.Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l).Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1",
keywords = "Ascorbic acid, dehydroascorbic acid, methotrexate, breast cancer, MDA-MB-231 cells, Reactive oxygen species. Intracellular oxidative stress",
author = "Yewande Dosunmu and Richard Owusu-Apenten",
doi = "10.9734/JALSI/2017/37185",
journal = "Journal of Applied Life Sciences International",
publisher = "SCIENCEDOMAIN International",
Dosunmu, Y & Owusu-Apenten, R 2017, 'Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability', Journal of Applied Life Sciences International, vol. 14, no. 2. https://doi.org/10.9734/JALSI/2017/37185
Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability. / Dosunmu, Yewande; Owusu-Apenten, Richard.
In: Journal of Applied Life Sciences International, Vol. 14, No. 2, 28.10.2017.
T1 - Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability
AU - Dosunmu, Yewande
AU - Owusu-Apenten, Richard
N2 - Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate(MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS).Study Design: In-vitro method.Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2',7'-dichlorofluroscein diacetate assay.Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l).Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1
AB - Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate(MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS).Study Design: In-vitro method.Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2',7'-dichlorofluroscein diacetate assay.Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l).Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1
KW - dehydroascorbic acid
KW - methotrexate
KW - breast cancer
KW - MDA-MB-231 cells
KW - Reactive oxygen species. Intracellular oxidative stress
U2 - 10.9734/JALSI/2017/37185
DO - 10.9734/JALSI/2017/37185
JO - Journal of Applied Life Sciences International
JF - Journal of Applied Life Sciences International
Dosunmu Y, Owusu-Apenten R. Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability. Journal of Applied Life Sciences International. 2017 Oct 28;14(2). https://doi.org/10.9734/JALSI/2017/37185