Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells
Min S. Park, Dale L. Ludwig, Evelyn Stigger, Suk-Hee Lee
The yeast RAD52 protein is essential for DNA double-strand break repair, and meiotic and mitotic recombination. RPA is a protein complex of three subunits (70, 34, and 11 kDa) that has been shown to be involved in DNA replication, nucleotide excision repair, and homologous recombination. Here, we demonstrate a physical interaction between human RAD52 and RPA in vivo and in vitro. In addition, the domain (amino acids 221-280) in RAD52 protein that mediates the interaction with the 34-kDa subunit of RPA was also determined. Overexpression of mutant RAD52 proteins lacking the interaction domain (amino acids 221-240, 241-260, and 261-280) failed to induce homologous recombination in monkey cells. We have previously shown that overexpression of human RAD52 induced homologous recombination in these cells. These results suggest that direct physical interactions between RAD52 and RPA are essential for homologous recombination in mammalian cells.
Protein Interaction Domains and Motifs
Fungal Proteins
Mutant Proteins
Park, M. S., Ludwig, D. L., Stigger, E., & Lee, S-H. (1996). Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. Journal of Biological Chemistry, 271(31), 18996-19000. https://doi.org/10.1074/jbc.271.31.18996
Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. / Park, Min S.; Ludwig, Dale L.; Stigger, Evelyn; Lee, Suk-Hee.
In: Journal of Biological Chemistry, Vol. 271, No. 31, 1996, p. 18996-19000.
Park, MS, Ludwig, DL, Stigger, E & Lee, S-H 1996, 'Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells', Journal of Biological Chemistry, vol. 271, no. 31, pp. 18996-19000. https://doi.org/10.1074/jbc.271.31.18996
Park MS, Ludwig DL, Stigger E, Lee S-H. Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. Journal of Biological Chemistry. 1996;271(31):18996-19000. https://doi.org/10.1074/jbc.271.31.18996
Park, Min S. ; Ludwig, Dale L. ; Stigger, Evelyn ; Lee, Suk-Hee. / Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 31. pp. 18996-19000.
@article{0a111b238dcc47859a5c9ad4aae84298,
title = "Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells",
abstract = "The yeast RAD52 protein is essential for DNA double-strand break repair, and meiotic and mitotic recombination. RPA is a protein complex of three subunits (70, 34, and 11 kDa) that has been shown to be involved in DNA replication, nucleotide excision repair, and homologous recombination. Here, we demonstrate a physical interaction between human RAD52 and RPA in vivo and in vitro. In addition, the domain (amino acids 221-280) in RAD52 protein that mediates the interaction with the 34-kDa subunit of RPA was also determined. Overexpression of mutant RAD52 proteins lacking the interaction domain (amino acids 221-240, 241-260, and 261-280) failed to induce homologous recombination in monkey cells. We have previously shown that overexpression of human RAD52 induced homologous recombination in these cells. These results suggest that direct physical interactions between RAD52 and RPA are essential for homologous recombination in mammalian cells.",
author = "Park, {Min S.} and Ludwig, {Dale L.} and Evelyn Stigger and Suk-Hee Lee",
doi = "10.1074/jbc.271.31.18996",
pages = "18996--19000",
T1 - Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells
AU - Park, Min S.
AU - Ludwig, Dale L.
AU - Stigger, Evelyn
AU - Lee, Suk-Hee
N2 - The yeast RAD52 protein is essential for DNA double-strand break repair, and meiotic and mitotic recombination. RPA is a protein complex of three subunits (70, 34, and 11 kDa) that has been shown to be involved in DNA replication, nucleotide excision repair, and homologous recombination. Here, we demonstrate a physical interaction between human RAD52 and RPA in vivo and in vitro. In addition, the domain (amino acids 221-280) in RAD52 protein that mediates the interaction with the 34-kDa subunit of RPA was also determined. Overexpression of mutant RAD52 proteins lacking the interaction domain (amino acids 221-240, 241-260, and 261-280) failed to induce homologous recombination in monkey cells. We have previously shown that overexpression of human RAD52 induced homologous recombination in these cells. These results suggest that direct physical interactions between RAD52 and RPA are essential for homologous recombination in mammalian cells.
AB - The yeast RAD52 protein is essential for DNA double-strand break repair, and meiotic and mitotic recombination. RPA is a protein complex of three subunits (70, 34, and 11 kDa) that has been shown to be involved in DNA replication, nucleotide excision repair, and homologous recombination. Here, we demonstrate a physical interaction between human RAD52 and RPA in vivo and in vitro. In addition, the domain (amino acids 221-280) in RAD52 protein that mediates the interaction with the 34-kDa subunit of RPA was also determined. Overexpression of mutant RAD52 proteins lacking the interaction domain (amino acids 221-240, 241-260, and 261-280) failed to induce homologous recombination in monkey cells. We have previously shown that overexpression of human RAD52 induced homologous recombination in these cells. These results suggest that direct physical interactions between RAD52 and RPA are essential for homologous recombination in mammalian cells.
U2 - 10.1074/jbc.271.31.18996
DO - 10.1074/jbc.271.31.18996
SP - 18996
EP - 19000
10.1074/jbc.271.31.18996