Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis
Junlan Zhou, Min Cheng, Min Wu, Chan Boriboun, Kentaro Jujo, Shiyue Xu, Ting C. Zhao, Yao Liang Tang, Raj Kishore, Gangjian Qin
The growth of new blood vessels after ischemic injury requires endothelial cells (ECs) to divide and proliferate, and the E2F transcription factors are key regulators of the genes responsible for cell-cycle progression; however, the specific roles of individual E2Fs in ECs are largely unknown. To determine the roles of E2F2 and E2F3 in EC proliferation and the angiogenic response to ischemic injury, hind-limb ischemia was surgically induced in E2F2-/- mice, endothelial-specific E2F3-knockout (EndoE2F3{increment}/{increment}) mice, and their littermates with wild-type E2F2 and E2F3 expression. Two weeks later, Laser-Doppler perfusion measurements, capillary density, and endothelial proliferation were significantly greater in E2F2-/- mice and significantly lower in EndoE2F3{increment}/{increment} mice than in their littermates, and EndoE2F3{increment}/{increment} mice also developed toe and limb necrosis. The loss of E2F2 expression was associated with increases in the proliferation and G1/S-phase gene expression of isolated ECs, while the loss of E2F3 expression led to declines in these parameters. Thus E2F2 impairs, and endothelial E2F3 promotes, the angiogenic response to peripheral ischemic injury through corresponding changes in EC cell-cycle progression.
Journal of molecular and cellular cardiology
https://doi.org/10.1016/j.yjmcc.2013.04.009
E2F Transcription Factors
G1 Phase
Regulator Genes
S Phase
E2F
Zhou, J., Cheng, M., Wu, M., Boriboun, C., Jujo, K., Xu, S., ... Qin, G. (2013). Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis. Journal of molecular and cellular cardiology, 60(1), 68-71. https://doi.org/10.1016/j.yjmcc.2013.04.009
Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis. / Zhou, Junlan; Cheng, Min; Wu, Min; Boriboun, Chan; Jujo, Kentaro; Xu, Shiyue; Zhao, Ting C.; Tang, Yao Liang; Kishore, Raj; Qin, Gangjian.
In: Journal of molecular and cellular cardiology, Vol. 60, No. 1, 01.07.2013, p. 68-71.
Zhou, J, Cheng, M, Wu, M, Boriboun, C, Jujo, K, Xu, S, Zhao, TC, Tang, YL, Kishore, R & Qin, G 2013, 'Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis', Journal of molecular and cellular cardiology, vol. 60, no. 1, pp. 68-71. https://doi.org/10.1016/j.yjmcc.2013.04.009
Zhou J, Cheng M, Wu M, Boriboun C, Jujo K, Xu S et al. Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis. Journal of molecular and cellular cardiology. 2013 Jul 1;60(1):68-71. https://doi.org/10.1016/j.yjmcc.2013.04.009
Zhou, Junlan ; Cheng, Min ; Wu, Min ; Boriboun, Chan ; Jujo, Kentaro ; Xu, Shiyue ; Zhao, Ting C. ; Tang, Yao Liang ; Kishore, Raj ; Qin, Gangjian. / Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis. In: Journal of molecular and cellular cardiology. 2013 ; Vol. 60, No. 1. pp. 68-71.
@article{967e72f33bea4924934cb58494f1ed7c,
title = "Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis",
abstract = "The growth of new blood vessels after ischemic injury requires endothelial cells (ECs) to divide and proliferate, and the E2F transcription factors are key regulators of the genes responsible for cell-cycle progression; however, the specific roles of individual E2Fs in ECs are largely unknown. To determine the roles of E2F2 and E2F3 in EC proliferation and the angiogenic response to ischemic injury, hind-limb ischemia was surgically induced in E2F2-/- mice, endothelial-specific E2F3-knockout (EndoE2F3{increment}/{increment}) mice, and their littermates with wild-type E2F2 and E2F3 expression. Two weeks later, Laser-Doppler perfusion measurements, capillary density, and endothelial proliferation were significantly greater in E2F2-/- mice and significantly lower in EndoE2F3{increment}/{increment} mice than in their littermates, and EndoE2F3{increment}/{increment} mice also developed toe and limb necrosis. The loss of E2F2 expression was associated with increases in the proliferation and G1/S-phase gene expression of isolated ECs, while the loss of E2F3 expression led to declines in these parameters. Thus E2F2 impairs, and endothelial E2F3 promotes, the angiogenic response to peripheral ischemic injury through corresponding changes in EC cell-cycle progression.",
keywords = "Angiogenesis, E2F, Endothelial cells, Ischemia, Proliferation",
author = "Junlan Zhou and Min Cheng and Min Wu and Chan Boriboun and Kentaro Jujo and Shiyue Xu and Zhao, {Ting C.} and Tang, {Yao Liang} and Raj Kishore and Gangjian Qin",
doi = "10.1016/j.yjmcc.2013.04.009",
journal = "Journal of Molecular and Cellular Cardiology",
T1 - Contrasting roles of E2F2 and E2F3 in endothelial cell growth and ischemic angiogenesis
AU - Zhou, Junlan
AU - Cheng, Min
AU - Wu, Min
AU - Boriboun, Chan
AU - Jujo, Kentaro
AU - Xu, Shiyue
AU - Zhao, Ting C.
AU - Tang, Yao Liang
AU - Kishore, Raj
AU - Qin, Gangjian
N2 - The growth of new blood vessels after ischemic injury requires endothelial cells (ECs) to divide and proliferate, and the E2F transcription factors are key regulators of the genes responsible for cell-cycle progression; however, the specific roles of individual E2Fs in ECs are largely unknown. To determine the roles of E2F2 and E2F3 in EC proliferation and the angiogenic response to ischemic injury, hind-limb ischemia was surgically induced in E2F2-/- mice, endothelial-specific E2F3-knockout (EndoE2F3{increment}/{increment}) mice, and their littermates with wild-type E2F2 and E2F3 expression. Two weeks later, Laser-Doppler perfusion measurements, capillary density, and endothelial proliferation were significantly greater in E2F2-/- mice and significantly lower in EndoE2F3{increment}/{increment} mice than in their littermates, and EndoE2F3{increment}/{increment} mice also developed toe and limb necrosis. The loss of E2F2 expression was associated with increases in the proliferation and G1/S-phase gene expression of isolated ECs, while the loss of E2F3 expression led to declines in these parameters. Thus E2F2 impairs, and endothelial E2F3 promotes, the angiogenic response to peripheral ischemic injury through corresponding changes in EC cell-cycle progression.
AB - The growth of new blood vessels after ischemic injury requires endothelial cells (ECs) to divide and proliferate, and the E2F transcription factors are key regulators of the genes responsible for cell-cycle progression; however, the specific roles of individual E2Fs in ECs are largely unknown. To determine the roles of E2F2 and E2F3 in EC proliferation and the angiogenic response to ischemic injury, hind-limb ischemia was surgically induced in E2F2-/- mice, endothelial-specific E2F3-knockout (EndoE2F3{increment}/{increment}) mice, and their littermates with wild-type E2F2 and E2F3 expression. Two weeks later, Laser-Doppler perfusion measurements, capillary density, and endothelial proliferation were significantly greater in E2F2-/- mice and significantly lower in EndoE2F3{increment}/{increment} mice than in their littermates, and EndoE2F3{increment}/{increment} mice also developed toe and limb necrosis. The loss of E2F2 expression was associated with increases in the proliferation and G1/S-phase gene expression of isolated ECs, while the loss of E2F3 expression led to declines in these parameters. Thus E2F2 impairs, and endothelial E2F3 promotes, the angiogenic response to peripheral ischemic injury through corresponding changes in EC cell-cycle progression.
KW - E2F
KW - Ischemia
U2 - 10.1016/j.yjmcc.2013.04.009
DO - 10.1016/j.yjmcc.2013.04.009
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
10.1016/j.yjmcc.2013.04.009