<p>Hi,</p>

<p>I used CCAT to call peaks for the H3k4me3 mark in my samples and the average peak width I noticed is ~3kb</p>

<p>I wanted to get a sense check on these nos. ? Usually in cancer samples, is that the sort of profile that is seen for H3k4me3. I tried looking this up in literature but I dint get enough data pts and the few that I did showed an average profile of 1.5kb.</p>

<p>Does anybody have any references on this ?</p>

<p>Thanks !</p>

<p>From H3K4me3 in mESC (mouse embryonic stem cells), what I know is that the promoters which have the tri-methylated nuclesomes at active. This width, I plot as an average profile in and around +/-3KB of TSS, which is sufficient to answer. Its hard to say the exact width, but I would assume it to be a max of 3KB so it might be fine.</p>

<p>As an example, I took this screenshot from the <a href='http://cistrome.org/cr/help.php'>cistrome</a> webpage. Its obvious that the average width would be around 2-3KB but I am not sure if that be different in the cancer cell line.</p>

<p><img src='http://s30.postimg.org/6hiteesj5/Screen_Shot_2014_03_07_at_23_38_36.png' alt='TSS H3K4me3' /></p>
