Hi All,

Is it possible to re-run STAR RNA aligner to obtain wiggle plots on already aligned and sorted BAM files? Or do I need to re-align again to obtain this type of output? I really do not want to have to re-align - so I am open to other options.

Thank you!


See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4631051/

    STAR --runMode inputAlignmentsFromBAM \
    --inputBAMfile Aligned.sortedByCoord.out.bam \
    --outWigType bedGraph \
    --outWigStrand Stranded