Hello, everyone !

I'm looking for a way to use HTseq with multi-thread.

I couldn't find any options about multi-thread.

Anybody knows how to, please ?

(I know there are tools support multi-thread like STAR, HISAT2. but just wonder whether HTseq doesn't support it.)

Thank you. : )
HTSeq-count (I guess that is what you are referring to) doesn`t support it as of now for a single file, see the issue for the  discussion and alternatives: https://github.com/htseq/htseq/issues/7#issuecomment-638075405

To be precise (citing the developer)
> That's it. The -n can process up to n BAM files in parallel to speed up, but cannot use multiple cores on the same file.

Other HTSeq commands are muti-threaded and support the `-n` option though.

You could use featureCounts instead if you really need multi-threading support. Or run HTSeq count through `parallel` on multiple files, but this will not scale favorably complexity-wise (duplicated coordinate parsing, duplicated tree structures, increased simultaneous file IO), so this is not recommended.

