I just got my RNA seq data.

CZ1_S6_L001_R1_001.fastq

CZ1_S6_L002_R1_001.fastq

CZ1_S6_L003_R1_001.fastq

CZ1_S6_L004_R1_001.fastq

I only know there are from 4 different lanes during sequencing.

What are they and do I need to combine these fastq files into one? And how?

Thanks~~~
It appears to be the same sample run in 4 separate lanes. You can `cat` the files together into one or process them independently (giving you a way to parallelize). You can merge the 4 sample bam files into one (and then sort) at the end.