Hi pals, I got some problems here.

I have an excel file with several genomic coordinates in the format "strand:start:end", and I also have access to my genome of interest in different formats (fna, gbk...). Now, what I want to do is to extract and obtain a file with all the sequences corresponding to my coordinates, I don't mind if the sequences have a header or a custom ID, I just want the sequences in the same order as I have them in my excel file. Is there any tool that can do this? It would be much appreciated since I have to extract more than 2000 sequences, and doing this manually is humanly impossible.

The next step is to perform a BLAST with another genome, also accessible in several formats. I'm using the blastn NCBI program and this script `-task blastn -query "query" -subject "subject" -oytfmt 6 >name`, how can I make the output file to only contain the most relevant matches? (E.g. only the matches that have an E value lower than 0.0001)

Thanks beforehand!
Save your excel data as tab delimited file.

Use [bedtools getfasta][1] to fetch sequences:

    bedtools getfasta [OPTIONS] -fi <input FASTA> -bed <BED/GFF/VCF> -fo <output FASTA>

 [1]: https://bedtools.readthedocs.org/en/latest/content/tools/getfasta.html