I'm wondering if anyone knows how I can get the trace data for each base, formatted into a text file. I've tried EMBOSS abiview and Biopython to retrieve these data. EMBOSS generates four file, such as suggested here, but the values don't seem to correlate to the chromatogram and trace values such as in the image. Biopython works to retrieve the data as dictionaries, but the in the dictionary `abif_raw`, the keys DATA9-12 are missing.

Chromatogram example with trace values:

https://ibb.co/dmpMMm
[Tracy][1] can do this.

    ./tracy basecall -f tsv -o <out.tsv> <trace.ab1>

The tab-delimited output file lists the trace with the basecalls. 

There is also an [online method][2] to visualize the trace on [GEAR][3].

  [1]: https://github.com/gear-genomics/tracy
  [2]: https://gear.embl.de/teal
  [3]: https://gear.embl.de/
