Hi All,

Is it possible to re-run STAR RNA aligner to obtain wiggle plots on already aligned and sorted BAM files? Or do I need to re-align again to obtain this type of output? I really do not want to have to re-align - so I am open to other options.

Thank you!


`bamCoverage` from deepTools would be the quickest method to do this. You can also have it output bigWig rather than wiggle files, which is generally preferable (you can directly load those in IGV, for example).