Hi all,

I'm trying to use Salmon in a differential expression analysis, for this i need a transcriptome fasta.

Given the fact that there is only a genome available for my species, i need to generate the transcriptome (the gene annotation is available for my species, in GFF3 format).

Does anyone know a good way to do that?

Thank you in advance for you advices
`rsem-prepare-reference` from RSEM ( https://www.biostars.org/p/260395/#260409 ), or `gffread` from StringTie - I prefer the later.