Dear Biostar Community,

What is the best tool for read generation from given reference sequences or any that you use/used and it works/worked as expected?

The properties of desired tool: 

- reads generate of given insert size and read length with uniform coverage
- handle uniform coverage on circle genomes 
- handle large genomes without crashing
- generation of Illumina reads
- generation of PacBio reads
- generation of reads without errors
- generation of reads contaminated by technical sequences
- generation of read-through adapters artefacts

I used ART (http://www.niehs.nih.gov/research/resources/software/biostatistics/art/) but it looks like it uses too old model for Illumina.

[randomreads][1] from BBMap. <br>PacBio reads can be generated [this][2] way.


  [1]: http://seqanswers.com/forums/showthread.php?t=48988
  [2]: https://sourceforge.net/p/bbmap/discussion/general/thread/25863b5d/#738f/91e5