Hi pals, I got some problems here.

I have an excel file with several genomic coordinates in the format "strand:start:end", and I also have access to my genome of interest in different formats (fna, gbk...). Now, what I want to do is to extract and obtain a file with all the sequences corresponding to my coordinates, I don't mind if the sequences have a header or a custom ID, I just want the sequences in the same order as I have them in my excel file. Is there any tool that can do this? It would be much appreciated since I have to extract more than 2000 sequences, and doing this manually is humanly impossible.

The next step is to perform a BLAST with another genome, also accessible in several formats. I'm using the blastn NCBI program and this script `-task blastn -query "query" -subject "subject" -oytfmt 6 >name`, how can I make the output file to only contain the most relevant matches? (E.g. only the matches that have an E value lower than 0.0001)

Thanks beforehand!
If you're using Windows, save your excel file as text with tab delimiters, and first column chromosome name, second start, third end (this is BED format. Then install Python3, then open `cmd.exe:`

```
C:\Python34\Scripts\pip.exe install pyfaidx
C:\Python34\Scripts\faidx.exe genome.fna --bed regions.bed > out.fasta
```