Dear all, 

please could you advise, what is a typical range of AF (allele fractions) of somatic mutations in the cancer samples in order to consider them clonal or sub-clonal ? Thank you ...

-- bogdan 

In addition to Kevin's answer, [Quantification of subclonal selection in cancer from bulk sequencing data][1] and references therein may be another useful read.

If you want some sensible cutoff for some rough analysis or filtering, I would say 20% AF is about right, but again, as Kevin says it depends...


  [1]: https://www.ncbi.nlm.nih.gov/pubmed/29808029