Hi all

I am new to Biostars and blast.
I am trying to convert a (test)-database of 25 sequences into a blast-database using standard tutorial commands. However, I get the following error: 

 *Error: mdb_env_open: There is not enough space on the disk.*

In my cd I get a file of >200gb which seems ridiculously high for only 25 sequences. Does anyone have an idea what I am doing wrong?

Kind regards

Michaël
Response from NLM support solved the issues. Here it is.

Thank you for the report.  This is a known issue with the Windows release. The program makeblastdb attempts to allocate a very large amount of virtual memory.  You can solve the problem by setting the a new BLAST environment variable
BLASTDB_LMDB_MAP_SIZE=1000000
See the BLAST setup documentation for details on how to set Windows environment variables (https://www.ncbi.nlm.nih.gov/books/NBK52637/). Once you change the variable, you'll need to close and reopen the command window where you were running BLAST for the new setting to take effect.