<p>Hi,</p>

<p>I want to ask in analysis of rnaseq data to detect differential expressed genes, etc., can technical replicates and biological replicates put together to compare?</p>

<p>Thanks,</p>

<p>Ch</p>

<p>The standard recommendation is to combine your technical replicates into one "lane" by simply adding their reads together. Use your biological replicates to estimate variance/dispersion.</p>
