Hello,
I have 11 proteins in two datasets: with 5 in 1st and 6 in 2nd dataset. I have calculated the amino acid percentage for three amino acids (A, R, N) in them as shown in the attached file from excel ![enter image description here][1]

I want to normalize them by background amino acid probability of all proteins in two datasets and then conclude, for e.g., basic amino acids (R) are enriched or
hydrophobic amino acids (A) enriched. Kindly help.

Thank you!


  [1]: http://i.imgur.com/X1pzo09.jpg
Percentages and frequencies are already a form of normalization, aren't they?

May be what you mean is how to test whether proportions of certain types of aa are significantly different in the two sets . A Fisher's exact test will do the job.

