Hi all,

I want to do read normalization using trinity, actually with looking the written command in the trinity's site, I'm confused about the allocated RAM to Jellyfish and coverage. It says:

```
TRINITY_RNASEQ_ROOT/util/insilico_read_normalization.pl \
  --seqType fq \
  --JM 100G \
  --max_cov 30 \
  --left left.fq \
  --right right.fq \
  --pairs_together \
  --PARALLEL_STATS \
  --CPU 10
```

Could anybody help me to know what `--JM 100G --max_cov 30` means. From what I understand, 100GB RAM is allocated to Jellyfish that seems is high for computer with 64GB RAM. please let me know the exact command for read normalization in my computer (64GB RAM). Thank a lot in advance
If you want to normalize reads and assemble the transcript with normalized reads (default 50x coverage):

    TRINITY_RNASEQ_ROOT/Trinity --normalize_reads --seqType fq --JM 60G --left left.fq --right right.fq --CPU 6

If you want to change the parameter, say `--normalize_max_read_cov` to `30`, add the following to the above command: `--normalize_max_read_cov 30`