Dear All,

I am doing few histone protein's SNP analysis.

I have used SIFT and Polyphen for my initial analysis. Now I would like predict its functional impact.

Can anyone please suggest me few tools or software for the prediction of the functional

effect of nsSNPs. Thank you
You can try [SnpEff][1] + SnpFit (dbNFSP)

NOTE: RamRs noted that the input data format is NOT from a sequencing experiment, but another format that lacks of the corresponding genomic coordinates. In such case, this recommendation is not appropriate for the analysis.

 [1]: http://snpeff.sourceforge.net/