Hello All,

I have one eight lane HiSeq-4000 flow cell data.I want to demultiplex only lane 5,6,7,8 and skip other lanes.I made a sample sheet with only lane 5,6,7 & 8 and processed the bcl2fastq by the following command. But it generate lane 5,6,7 & 8 lane data along with Undetermined fastq file of lane 1,2,3 & 4.It is time more time & space consuming process.Please let me know that Is there any way to skip the unwanted line? So we can save time and space while doing bcl2fast.

Command Used: 

    bcl2fastq --input-dir 151112_K00155_0019_BH573GBBXX/Data/Intensities/BaseCalls/ --runfolder-dir 151112_K00155_0019_BH573GBBXX/ --sample-sheet Samplesheet.csv --output-dir unaligned --use-bases-mask Y*,I8n,Y* --barcode-mismatches 1 -l DEBUG &
Are you using bcl2fastq2? If so, I think you could use the `--tiles` parameter:

    --tiles s_[5-8]

**edit**: I've updated my answer according to Devon Ryan comment bellow, previously it was `--tiles s[5-8]`