I am wondering could anyone tell me the best work flow (software) and Statistical model should I use for RNA seq data (without replications).

Thanks a lot
<p>The best tool you&#39;ll find is GFold, and you should obviously be very cognizant of the caveats that come with trying to draw any firm conclusions from unreplicated data (i.e., the most this is useful for is getting some target genes for replicated targeted tests).</p>
