Hi friends

I want to use DESeq2 to normalize the raw count data to do PCA.
I dont have colData. 

What code should I use? because in the DESeq2 workflow we need colData and design.
thanks
You can Google the size normalization algorithm and do it yourself.

Or, make fake coldata, and a design of 1, and then you can make a valid DESeq2 object.