Hi Biostar,

Apologies for this basic question but I can not find a simple answer

I want to sequence several samples of mRNA paired end 2x150 and for each sample I want 100 million reads (100 million for forward and 100 million for reverse)

the supplier proposes to sequence 3 samples per lane of flow cell of the Hiseq 4000 technology.
So what I understood 3 samples x 200 million reads (100 million for forward and 100 million for reverse) = 600 million reads per lane


but when I looked for the performances Illumina Hiseq 4000 I found this technology can generate 312000000 Clusters per lane only. How can this technology generate 600 million reads per lane ??

Would it be possible to give me a simple answer Thank you in advance
Illumina traditionally counts two reads coming from a single cluster independently. `312000000` passing clusters give rise to `312000000 x 2 = Number of reads` per lane. 

Please keep in mind this number if attainable with `excellent` quality genomic DNA libraries. Any deviation from this will result in lower yields.