**Hi Everyone** 

I am trying to use Michigan Impute Server. I use Checkvcf first to avoid failure in the server. 

    python checkVCF.py -r checkVCF/hs37d5.fa -o test chr3.vcf

**I got some duplicates and inconsistent ref.** 


    > checkVCF.py -- check validity of VCF file for meta-analysis version
    > 1.4 (20140115) contact zhanxw@umich.edu or dajiang@umich.edu for problems. Python version is [ 2.7.5.final.0 ]  Begin checking vcfFile
    > [ chr3.vcf ] Duplicated site [ 3:14187449 ] Duplicated site [
    > 3:21307401 ] Duplicated site [ 3:38608045 ] Duplicated site [
    > 3:39146429 ] Duplicated site [ 3:41912651 ] [ 10000 ] lines processed 
    > Duplicated site [ 3:48618728 ] Duplicated site [ 3:79399575 ]
    > Duplicated site [ 3:95176677 ] Duplicated site [ 3:96472739 ]
    > Duplicated site [ 3:99067458 ] [ 20000 ] lines processed  Duplicated
    > site [ 3:113876275 ] Duplicated site [ 3:120522716 ] Duplicated site [
    > 3:121633904 ] Duplicated site [ 3:128622922 ] [ 30000 ] lines
    > processed  Duplicated site [ 3:171926373 ] Duplicated site [
    > 3:183371250 ]
    > ---------------     REPORT     --------------- Total [ 37146 ] lines processed Examine [ 33 ] VCF header lines, [ 37113 ] variant sites, [
    > 378 ] samples [ 16 ] duplicated sites [ 0 ] NonSNP site are outputted
    > to [ test.check.nonSnp ] [ 6995 ] Inconsistent reference sites are
    > outputted to [ test.check.ref ] [ 0 ] Variant sites with invalid
    > genotypes are outputted to [ test.check.geno ] [ 0 ] Alternative
    > allele frequency > 0.5 sites are outputted to [ test.check.af ] [ 0 ]
    > Monomorphic sites are outputted to [ test.check.mono ]
    > ---------------     ACTION ITEM     ---------------
    > * Remove duplicated sites and rerun checkVCF.py
    > * Read test.check.ref, for autosomal sites, make sure the you are using the forward strand
    > * Upload these files to the ftp server (so we can double check): test.check.log test.check.dup test.check.noSnp test.check.ref
    > test.check.geno test.check.af test.check.mono


How can I remove this **duplicate sites and inconsistent reference sites**? 

I tried this but it seems it excludes duplicate variants not sites: 

    plink --bfile snps_filtered --list-duplicate-vars ids-only suppress-first
    plink --bfile snps_filtered --exclude plink.dupvar --make-bed --out snps.DuplicatesRemoved 
    plink --bfile snps_filtered --recode vcf  --snps-only just-acgt  --out snps.final 

A link to where is this in plink will be okay too. 

**Thanks** 

I note that you are comparing to *hs37d5.fa*, but to which genome was the original sample aligned? It would likely be recorded in your VCF header.