I would like to know if there are tools or methods for adding a specific sequence into a fastA file at a specific position.

I would like to modify the genome, by adding a specific sequence in a specific location on the genome.

thanks

Assa
I did [this script][1] for one of my project some years ago. It allows you to look at bases at specific positions but also to modify your genome using another fasta file for the changes.

    python lookmod_genome.py -m modify -f file.fasta -c changes_file.fasta

The `changes_file.fasta` should contains all changes you want to make using 4 key words (insertion, deletion, add and remove) and should looks like this :

    >insertion:chr1:25:26
    GCTAGCTAGC
    >deletion:chr4:40:50
    >add:your_chromosome_name
    GTCGATCGTCATGGTT
    >remove:your_chromosome_name

I used it quite a lot but it's self made so check the result with the `look` mode

**Use Python 2 or modify the script to be Python 3 resilient**

  [1]: https://github.com/Baboon61/LAM/blob/master/lookmod_genome.py