Hey guys,

I am going to aligning reads from GSE35641 (http://www.ncbi.nlm.nih.gov/Traces/sra/?view=search_seq_name&exp=SRX119208&run=&m=search&s=seq), on ecoli reference genome (NC_000913). There are 6 sra files in GEO which the least size is more than 2G. Do you know any way to download the files faster? I need many hours to download a file of them in fasta format

Thank you biostars
Yep, install [Aspera][1] and run it as follows:

```
ascp -i $KEY/asperaweb_id_dsa.openssh -k 1 -QT -l 200m \
  anonftp@ftp-trace.ncbi.nlm.nih.gov:/sra/sra-instant/reads/ByRun/sra/SRR/SRR390/SRR390728/SRR390728.sra \ # url for the file
  ./ # save location
```

`$KEY` should point to the directory containing `asperaweb_id_dsa.openssh` (it will be in the installation directory). See instructions [here][2].

 [1]: http://downloads.asperasoft.com/en/downloads/8?list
 [2]: http://www.ncbi.nlm.nih.gov/books/NBK158899/#SRA_download.when_to_use_a_command_line