Hello.
I am trying to run a blast against NR locally, and I am looking to find a way to run it as fast as I can. I recalled from my memory that I used LC_ALL=C fgrep -wf- for a "quicker" search file from one file to another, so I'm wondering if I can do something similar with the blast.

In the same line with fgrep I tried 

blastp -query FILE -db LC_ALL=C /home/Protein_DataBases/nr/nr.fasta -evalue 1e-5 -outfmt 6 -num_threads 25 but it doesn't work. Does anyone knows if I can do this action??

Thank you in advance!

Don't put `LC_ALL=C` as a parameter to blast! That won't work. 

You can set LC_ALL=C before a command:

    LC_ALL=FOO; echo $LC_ALL

prints:

    FOO

it gets applied to all commands run in that shell.

The best recommendation would be to export the variable in bash (preferable upon initialization) so that it is always applied.

    export LC_ALL=C 

you may run into various problems otherwise when it comes to bioinformatics tools and processes. Specifically sorting will be byte-wise when is set to `C` and alphabetical otherwise.

