Hi there,

This is my first time performing chip-seq analysis. I would like to use SICER to identify enriched domains from histone modification data. One of the input parameters that SICER requires is the fragment size. From the manual:

> Fragment size: is for determination of the amount of shift from the beginning of a read to the center of the DNA fragment represented by the read. FRAGMENT_SIZE=150 means the shift is 75.

How can I calculate the fragment size?
<p>There is a script inside SICER download folder, called <code>fragment-size-estimation.sh </code>, you can try to use it to estimate the fragment size. I would recommend you to read <strong>SICER users</strong> google group. There are some interesting posts like: <a href="https://groups.google.com/forum/#!searchin/sicer-users/fragment/sicer-users/pWF0GmYYAMM/IISEiBArg18J">https://groups.google.com/forum/#!searchin/sicer-users/fragment/sicer-users/pWF0GmYYAMM/IISEiBArg18J</a> talking about fragment size estimation issue.<br />
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Hope it helps.</p>
