Hi everyone, i have a blast p result with the average identity percentage 35%, is it an acceptable percentage? 

If not what is the minimal acceptable identity percentage? 

Between Identity percentage, e-value and bitscore, which one should we focus on in order to find the best match? Thanks.
Identity 35% means that 35% of aa in your sequence match to other sequences in database. There isn't something like "acceptable percentage". It always depends on what you are looking for:
--- if you have unknown protein sequence and you would like to know the homology sequences, information about identity (even 35%)  is valuable,
--- if you have known protein and you need to confirm the sequence, the identity 35% is small and may suggest that something went wrong during your analysis. 

The E-value is very important, the lower the better. 

Best,

Agata
