Hello,

I have some Sanger sequences of HBB gene. I need to find variants related to Sickle Cell Anemia or Beta Thalassemia from these sequences. 

Is there any tool for calling variants from these sequences? Please help.

I have .ab1 files.

Thanks
You can call variants from *.ab1 files with [tracy][1] or the web application [indigo][2], a front end of tracy as described in [this paper][3] 


  [1]: https://github.com/gear-genomics/tracy
  [2]: https://www.gear-genomics.com/indigo/
  [3]: https://doi.org/10.1186/s12864-020-6635-8