Dear Biostars, Hi

I want to run **blastx** against **NCBI nr** for annotating **thousands** of *de novo* transcripts. I want to do it as fast as possible. 

I have heard that one way is to use Amazon cloud.
So, would you kindly help and introduce me any step-by-step manual or resource in this regard?

(e.g: where to begin? quantity of resources I need to rent from cloud and related costs? should I download the Nr database locally or downloading it into Amazon cloud?  or uploading my transcriptome assembly into the Amazon? is there any pre-installed and ready to use Cloud for Bioinfirmatics (some BioCloud)? . . .  and so on).

Thanks

**Notes:**

0- I have checked some [Biostars posts][1] but could not find any simple guidance.

1- I have no computational resources at home (no server and ...)

2- I do not want to use *Blast2GO Pro* CloudBlast (why? it is expensive for me!)


  [1]: https://www.biostars.org/p/7120/
Have you looked at the [NCBI BLAST on Cloud help][1]? There is step-by-step guidance available using links at the left.

That said, if you were looking to do this for thousands of transcripts then you should use [DIAMOND instead][2]. Regular blast+ may take too long and cost more.

There is also [PAUDA][3].


  [1]: https://ncbi.github.io/blast-cloud/
  [2]: https://github.com/bbuchfink/diamond
  [3]: https://ab.inf.uni-tuebingen.de/software/pauda