Datasets:

Shubhangi29

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llava_med_alignment_500k_chunk_2

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[ { "from": "human", "value": "Describe the image concisely.\n<image>" }, { "from": "gpt", "value": "Schematic representation of possible water-soluble gas sensing mechanism in CNF-film surface." } ]

[ { "from": "human", "value": "Characterize the image using a well-detailed description\n<image>" }, { "from": "gpt", "value": "Instruction-wise comparison of semantic effects (IncongruentSEM–CongruentSEM) at the subject NP. Difference waves (left column) and difference maps illustrate semantic congruency effects for the NeutralINST (black lines; upper row of maps) and for the PoetryINST (red lines; lower row of maps) conditions. Both conditions show a posterior negativity (N400) between 400 and 600 ms. The late positive response (P600) occurs earlier in the NeutralINST condition (600–700 ms) than in the PoetryINST condition (750–900 ms)." } ]

[ { "from": "human", "value": "Characterize the image using a well-detailed description\n<image>" }, { "from": "gpt", "value": "Comparison of brain region from mice between control group and nicotine group by applying PLS loading plots for the region 9.4–5.1 and 4.6–0.2 ppm (218 segments) after application of OSC data filter; (A) hippocampus in control group and nicotine group; (B) NAc in control group and nicotine group; (C) PFC in control group and nicotine group; (D) striatum in control group and nicotine group." } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "Genetic relationships based on minimum spanning tree of the 28 MLVA profiles of isolates from Guizhou province and isolates of worldwide distribution. Each circle corresponds to a MLVA profiles. The shadow zones in different colour correspond to different clonal complexes. The size of the circle is proportional to the number of the isolates, and the colour within the cycles represents the locality origin of Guizhou Province (red), other provinces of China (blue) or other countries (green)." } ]

[ { "from": "human", "value": "Provide a brief description of the given image.\n<image>" }, { "from": "gpt", "value": "Transthoracic echocardiography in uncomplicated type 1 BAV. Aortic short-axis view (a), mild aortic regurgitation (b), normal ascending aorta diameters (c), and absence of significant aortic stenosis (d)" } ]

[ { "from": "human", "value": "Break down the elements of the image in a detailed manner\n<image>" }, { "from": "gpt", "value": "Distance-based phylogenetic tree for the 28 annual plant species used to prepare the experimental scenarios. Based on “V.PhyloMaker” package in R. The capital letters between brackets next to the names of species indicate the species combinations in which they participated. In nonbold typeface, the high phylogenetic diversity scenarios (A and B combinations); in bold, the low phylogenetic diversity scenarios (C and D combinations)." } ]

[ { "from": "human", "value": "Describe the following image in detail\n<image>" }, { "from": "gpt", "value": "Ion signal depletion. Wavelength scans near 958 and 964 nm for seven different cluster sizes (solid symbols) together with the position of the resonance of the electronic transition for the bare C60+, taken from ref. 9 and corrected from air to vacuum (vertical lines). Error bars indicate the s.d. of the ion yield. Photoabsorption depletes the ion signal to minima at different wavelength positions with a line width of about 0.2 to 0.6 nm (full width at half maximum). The solid lines represent Lorentzian profiles fitted to the data." } ]

[ { "from": "human", "value": "Characterize the image using a well-detailed description\n<image>" }, { "from": "gpt", "value": "MoDCs at the pre-surgery timepoint. Cells were obtained at the seventh day of culture (after LPS addition) from (a) a healthy control; (b) a stage II-CRC patient; and (c) a stage III-CRC patient. The morphological analysis was performed by light microscopy (magnification, 100×) on MoDCs previously stained with May-Grünwald-Giemsa. A representative experiment is shown. MoDCs: monocyte-derived dendritic cells; LPS: lipopolysaccharide; CRC: colorectal cancer." } ]

[ { "from": "human", "value": "Offer a thorough analysis of the image\n<image>" }, { "from": "gpt", "value": "(A) Correlation between the mean concentration levels found in sludge and serum (A1) and placenta (B1). Stimulants (Caffeine, Theobromine, and Theophylline) were excluded due to their recent intake by human individuals before sample collection. (B) Venn diagram representing all features (combining positive and negative ionization modes) in common between human matrices and sludge. Only features with available MS/MS data were considered." } ]

[ { "from": "human", "value": "Clarify the contents of the displayed image with great detail\n<image>" }, { "from": "gpt", "value": "(a) The calculated geometric and electronic structure of 2-carboxy-d-arabinitol 1,5-bisphosphate (CABP); Q = −4, M = 1, E0 = −51,603.53 eV, G0 = −51,599.18 eV. Hessian has negative frequencies: −82.12, −18.16 (cm−1). (b) The scheme of 2-carboxy-2,3,4-trihydroxypentane-1,5-diyl diphosphate." } ]

[ { "from": "human", "value": "Describe the image concisely.\n<image>" }, { "from": "gpt", "value": "mRNA expression profile of SLC35D3 and adipogenic marker genes during intramuscular adipogenesis by RT-qPCR. GAPDH is the internal control. Data are shown as means ± S.E.M. n = 3" } ]

[ { "from": "human", "value": "Explain the various aspects of the image before you\n<image>" }, { "from": "gpt", "value": "Fourier Transform Mid Infrared (FTMIR) screening and categorization of 300 gamma-irradiated FTMIR spectral plots (1800 to 600 cm-1) of absorbance vs. wavenumbers of the 300 P. ovata M3 lines across the fingerprint region post data normalization. All 300 lines are represented in different colors. Wavenumbers highlighted with arrows are associated with vibrations of molecular functional groups present in xylan. Peaks with the most noticeable differences in absorbance are observed at 1376, 1365, 1312, 1131-1121, 1089-1070, 1010, 986 and 897 cm-1and valleys at 1348 and 856 cm-1. (B) Clustered heatmap-based comparisons of five absorbance ratios derived from (A). Regions of the main plot have been expanded to show mutants (indicated with asterisks) that were analyzed further in this study." } ]

[ { "from": "human", "value": "Relay a brief, clear account of the picture shown.\n<image>" }, { "from": "gpt", "value": "Test SCRIPS score, 0.5*recent case detection rate" } ]

[ { "from": "human", "value": "Write an exhaustive depiction of the given image\n<image>" }, { "from": "gpt", "value": "Effect of ELA on H/I-induced MSC apoptosis. a MSC apoptosis results obtained from flow cytometry of various experimental groups. b Apoptotic cell rates were statistically presented as mean ± SD. Three parallel experiments were conducted independently. *P < 0.05. ELA, ELABELA; MSC, mesenchymal stem cell; H/I, hypoxic/ischemic" } ]

[ { "from": "human", "value": "Present a compact description of the photo’s key features.\n<image>" }, { "from": "gpt", "value": "Concrete block II." } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "The effect of GIN pretreatment in vitro on metastatic potential in vivo PC-3M-Pro4luc2 cells were pretreated with vehicle or 100nM Gin for 48 hours prior to inoculation in the left cardiac ventricle of nude mice (n=9 per group). (a) Experimental schedule. (b) Quantification of the total number of metastases and (c) total tumor burden. ** p < 0.01 versus control." } ]

[ { "from": "human", "value": "Illustrate the image through a descriptive explanation\n<image>" }, { "from": "gpt", "value": "AP-1B expression in epithelial cells slows down migration speeds. LLC-PK1, MDCK, and HBE cells were grown in Matrigel-coated MatTek dishes typically for 2 d. After wounding, cells were transferred to a Nikon BioStation for live imaging for up to 4 h. (A) Selected still images of LLC-PK1::µ1A (top panels) and LLC-PK1::µ1B (bottom panels) wounded monolayers at the beginning (0 h), 2 h, and 4 h after the start of data acquisition. Images of selected areas at the wound edge were taken every 15 min. Pixeled lines indicate wound edges at the beginning of live imaging. Bars are 50 µm. (B and C) Traveled path length (B) and distance (C) of migrating LLC-PK1 cells were determined using manual tracking of individual cells at the wound edge as described in Materials and Methods. Data represent mean values from three independent experiments. LLC-PK1::µ1A: cells from nine different areas (a total of 55 individual cells) were analyzed; LLC-PK1::µ1B: cells from 14 different areas (a total of 70 individual cells) were analyzed. (D) Area closure data represent mean values from three independent experiments. LLC-PK1::µ1A: seven independent areas were analyzed; LLC-PK1::µ1B: 15 independent areas were analyzed. (E) 8 × 105 LLC-PK1::µ1A and LLC-PK1::µ1B were seeded on coverglass coated with 1 mg/ml Matrigel. After 48 h cells were trypsinized and counted to determine the time needed for cell doubling (two independent experiments). (F and G) Area closure analysis was performed on migrating LLC-PK1 cells stably expressing µ1A-HA or µ1B-HA (F) and µ1A-YFP or µ1B-YFP (G). Data represent mean values from at least three independent experiments. LLC-PK1::µ1A-HA: 15 independent areas; LLC-PK1::µ1B-HA: 32 independent areas; LLC-PK1::µ1A-YFP: 16 independent areas; and LLC-PK1::µ1B-YFP: 22 independent areas were analyzed. (H) Area closure analysis of mock or µ1B-depleted MDCK cells. Data represent mean values from at least three independent experiments using cells from three independent KDs. Control MDCK cells: 23 independent areas; µ1B-depleted MDCK cells: 25 independent areas were analyzed. (I) Migrating HBE cells depleted of ARH or GAPDH as control were analyzed for area closure during wound healing. Area closure data represent mean values of at least three independent experiments using cells from three individual KDs. GAPDH KD HBE cells: 40 independent areas; ARH KD HBE cells: 32 independent areas were analyzed. (H and I) Efficiency of KD was controlled for and specificity of KD was confirmed with RT-PCR (unpublished data). (B–I) Error bars indicate SD. ****P < 0.0001; ***P < 0.0003; **P < 0.006. KD, knockdown." } ]

[ { "from": "human", "value": "Write an exhaustive depiction of the given image\n<image>" }, { "from": "gpt", "value": "Scheme of early innate response to brain injury. Damage signals or DAMPs primarily released from the injured parenchymal cells are sensed by immune effector cells such as microglia, astrocytes, and macrophages. The triggered innate immune response (e.g., proinflammatory cytokines, chemokines, reactive oxygen species, excitotoxins, histamine, and prostaglandins) has detrimental influences on the neurons, oligodendroglial precursors, and vascular endothelial cells. The increased BBB permeability contributes the migration of peripheral immune cells (e.g., neutrophils, mast cells, and macrophages) to the sites of tissue damage." } ]

[ { "from": "human", "value": "Walk through the important details of the image\n<image>" }, { "from": "gpt", "value": "Effect of anti-CD16/CD32 Abs on CRP-induced MMP and TIMP-1 mRNA expression. Differentiated 3T3-L1 cells were cultured with 0 (control) or 50 µg/mL CRP in the presence or absence of anti-CD16/CD32 Abs for 12 h; the mRNA expression of seven MMPs (A-G) and TIMP-1 (H) was determined by real-time PCR. Each bar indicates the mean ± standard deviation (SD) of three independent experiments. **p < 0.01 (stimulation with CRP vs. control), †p < 0.05, p < 0.01 (stimulation with CRP vs. anti-CD16/32 Ab + stimulation with CRP)." } ]

[ { "from": "human", "value": "Offer a thorough analysis of the image\n<image>" }, { "from": "gpt", "value": "In vivo HA maps from all six subjects (2 × 2 × 2 mm3 interpolated to 1 × 1 × 1 mm3 for display), superposed to non-diffusion-weighted magnitude images and reformatted to XY, YZ and XZ view." } ]

[ { "from": "human", "value": "Examine the image closely and share its details\n<image>" }, { "from": "gpt", "value": "ABCA1 plays a key role in the inhibition of HCV infection. (A) Silencing of ABCA1. Huh7.5 cells were transfected with siRNA that targets ABCA1, or with a control siRNA. Total RNA was extracted after 48 h and ABCA1 mRNA determined by qRT-PCR. Results are expressed as the percentage of ABCA1 mRNA in cells transfected with siRNA targeting ABCA1 relative to mRNA levels in control cells. (B) Decreased ABCA1 protein synthesis in ABCA1-knocked-down cells. Huh7.5 cells were transfected with ABCA1-specific siRNA as above. Cell lysates were subjected to Western blot analysis. Staining of the ABCA1 protein with specific antibodies is shown in the insert. Results are expressed as the ABCA1 protein content in ABCA1-siRNA transfected cells relative to that in control siRNA transfected cells, normalized to calnexin (quantification using the Odyssey Infrared Imaging System). (C) Loss of cholesterol efflux function in ABCA1-silenced cells. Huh7.5 cells were transfected with siRNA that targets ABCA1 or with control siRNA. ABCA1-dependent [3H] cholesterol efflux was assayed in the presence of ApoA1. Results are expressed as percentage of cholesterol efflux to ApoA1 in ABCA1 knocked-down cells relative to control si-RNA transfected cells. (D) Silencing of ABCA1 antagonizes GW3965-mediated inhibition of HCV infection. The expression of ABCA1 was reduced by transfection with ABCA1-specific siRNA (as in A) and cells were treated with 1 µM GW3965, infected and grown for a further 24 h. HCV RNA was determined by qRT-PCR. Results are expressed as the percentage of HCV RNA in drug-treated (GW3965) cells or ABCA1-silenced and subsequently GW3965-treated cells (siABCA1+GW3965), compared to solvent-treated cells." } ]

[ { "from": "human", "value": "Analyze the image in a comprehensive and detailed manner\n<image>" }, { "from": "gpt", "value": "Tissue-specific predictions performance comparisons. Each plot shows the AUPR (area under the precision recall curve) on x axis and the AUROC (area under the receiver operating characteristics curve) on y axis. The size of each point represents the pearson correlation. (A) Performance on MPRA data in three cell lines (GM12878: 693 positive variants, 2772 control variants; HepG2: 524 positive variants, 1439 control variants; K562: 339 positive variants, 1361 control variants). (B) Performance on allele specific transcription factor binding SNVs (see the number of variants in Supplemental Table S1)." } ]

[ { "from": "human", "value": "Break down the elements of the image in a detailed manner\n<image>" }, { "from": "gpt", "value": "USP9X promotes breast carcinogenesis through regulating CEP131 abundance. (a) Colony formation assays of MCF-7 cells with Dox-inducible expression of USP9X or CEP131 in the absence or presence of Dox (upper panel). **P<0.01, Student's t-test. Colony formation assays of MCF-7 cells stably transfected with USP9X shRNA or CEP131 shRNA (lower panel). Representative images from biological triplicate experiments are shown. (b) MCF-7 cells stably transfected with USP9X shRNA or CEP131 shRNA were subjected to growth viability assay (left panel). Each bar represents the mean±s.d. for biological triplicate experiments. **P<0.01, two-way ANOVA. western blotting analysis of the indicated proteins is shown (right panel). (c) MCF-7 cells with Dox-inducible expression of CEP131 were stably transfected with USP9X shRNA for colony formation assays in the absence or presence of Dox. Representative images from biological triplicate experiments (left panel) and western blotting analysis of the indicated proteins (right panel) are shown. (d) MCF-7 infected with control lentiviruses or lentiviruses carrying shRNA against USP9X or CEP131 were transplanted on athymic mice (BALB/c; Charles River, Beijing, China) and tumour volumes were measured weekly. Each point represents the mean±s.d. for different animal measurements (n=6). **P<0.01, one-way ANOVA for tumour weight analysis and two-way ANOVA for tumour volume analysis. The levels of indicated proteins in these tumours were examined by western blotting. (e) USP9X-deficient MCF-7 tumours stably transfected with control vector or vector encoding CEP131 were transplanted onto athymic mice and tumour volumes were measured weekly. Each point represents the mean±s.d. for different animal measurements (n=6). *P<0.05; **P<0.01, one-way ANOVA for tumour weight analysis and two-way ANOVA for tumour volume analysis. The levels of indicated proteins in these tumours were examined by western blotting. (f) The xenograft tumours from four mice of each group as indicated were cultured and immunostained with antibodies against Centrin and USP9X. Scale bars, 10 μm. Representative images from two mice of each group are shown. More than one hundred cells from each xenograft were counted. Each bar represents the mean±s.d. for measurements of four xenografts in each group. *P<0.05, one-way ANOVA." } ]

[ { "from": "human", "value": "Write an exhaustive depiction of the given image\n<image>" }, { "from": "gpt", "value": "Myofibroblasts act as passive electrical paths for impulse conduction. (a) Heterocellular construct of a left-stimulated strand of cardiomyocytes (top) interrupt by a pure region (length = 134 μm) of myomesin-deficient myofibroblasts (bottom). Circles indicate the optical mapped area detected from each photodetector. (b) Optical action potential upstrokes recorded in a detected length of ~50 μm. (c) Activation times reconstruct from each action potential upstrokes; in both cardiomyocytes areas, the activation is almost immediate whereas the propagation into myofibroblast area exhibits a delay of 30 ms. (d) Summary of propagated delays related to the inserts' length, therefore indicating a passive electronic transmission of up to ~320 μm (modified with permission from [6])." } ]

[ { "from": "human", "value": "Provide a detailed description of the given image\n<image>" }, { "from": "gpt", "value": "A plain scan of the right lobe of the liver shows a low-density mass. The patient was male, 58 years old. A plain scan of the right lobe of the liver revealed a low-density mass of 10.1 cm x 11.5 cm. The CT value was approximately 49 HU (5A). The solid components in the arterial phase were significantly enhanced, and the CH value was approximately 91 HU (5 B). The CT value in the portal vein phase (5C) was 78 HU. Pathology (HE staining × 200) showed tumor thrombus in the small vein (5E)." } ]

[ { "from": "human", "value": "Examine the image closely and share its details\n<image>" }, { "from": "gpt", "value": "Brassica rapa chloroplast coverage pileup for the DRP000446 study. The gray spikes shows the counts of alignments at individual nucleotide positions (vertical scale is logarithmic). 27742 nucleotide positions of the total 180852 are covered. There are two regions around 100,000 and 135,000 where the coverage is more than 10 times than at other parts of the chloroplast. These are the regions where the ribosomal RNA genes are found which share very similar sequence with other chloroplasts and bacterial genomes. Indeed if we BLAST all the 1634 reads that matched the chloroplast genomes against the NCBI reference sequence database, 733 of them also match various bacterial genomes, but 897 does not match any other organisms, just plants. Removing those alignments that match bacterial genomes too, (gray spikes) makes the distribution more uniform." } ]

[ { "from": "human", "value": "Describe the image concisely.\n<image>" }, { "from": "gpt", "value": "Percentages of patients with different vitamin D levels." } ]

[ { "from": "human", "value": "Write an exhaustive depiction of the given image\n<image>" }, { "from": "gpt", "value": "(a) CV curves of the symmetric supercapacitor based on BSAC-A-800 using 17 m NaClO4 electrolyte measured at different voltage windows. (b) CV curves of the SSCs using 17 m NaClO4 electrolyte at different scan rates (10–100 mV s−1). (c) CV curves of SSCs using 17 m NaClO4 and 1 M Na2SO4 electrolyte at a scan rate of 10 mV s−1. (d) GCD curves of the SSCs using 17 m NaClO4 electrolyte at various current densities. (e) Galvanostatic charge–discharge curves of SSCs using 17 m NaClO4 and 1 M Na2SO4 electrolyte at the current densities of 0.5 A g−1. (f) Specific capacitances of the SSCs using 17 m NaClO4 electrolyte at different current densities." } ]

[ { "from": "human", "value": "Provide a brief description of the given image.\n<image>" }, { "from": "gpt", "value": "a) Modification of molecular symmetry. b) Initial structural phase transition. c) Modified phase transition. Reproduced with permission.[ ] Copyright 2019, American Chemical Society." } ]

[ { "from": "human", "value": "Present a compact description of the photo’s key features.\n<image>" }, { "from": "gpt", "value": "Echo app: deleting the account." } ]

[ { "from": "human", "value": "Walk through the important details of the image\n<image>" }, { "from": "gpt", "value": "PM-activated TRPM2 promotes ZO-1 degradation by calpain. (A) Human lung microvascular ECs grown in 6-well dishes to approximately 95% confluence were treated with TRPM2 antibody or control IgG (5 μg/ml) for 4 hr, and then challenged with PM (100 μg/ml) for 6 hr. Cell lysates were analyzed by Western blotting with ZO-1 antibody. Changes in levels of ZO-1 are expressed as fold changes and normalized to β-actin. Shown are representative blots from three independent experiments. *p < 0.05 compared to control. **p < 0.05 compared to PM challenge. (B) ECs grown in 6-well dishes to approximately 80% confluence were treated with TRPM2 siRNA or control siRNA (100 ng/ml) for 48 hr, and cell lysates were analyzed by Western blotting with ZO-1 antibody. (C) ECs grown in 6-well dishes to approximately 95% confluence were treated with TRPM2 siRNA or control siRNA (100 ng/ml) for 48 hr, and then challenged with PM (100 μg/ml) for 6 hr. Cell lysates were analyzed by Western blotting with ZO-1 antibody. Changes in levels of ZO-1 are expressed as fold changes and normalized to β-actin. Shown are representative blots from three independent experiments. *p < 0.05 compared to control. **p < 0.05 compared to PM challenge. (D) ECs grown on ECIS gold electrodes were treated with TRPM2 antibody or control IgG (5 μg/ml) for 4 hr, and then challenged with PM (100 μg/ml). Changes in TER were measured with ECIS. *p < 0.05 compared to PM-challenged group. (E) ECs grown on 100 mm dishes were treated with TRPM2 siRNA or control siRNA (100 ng/ml) for 48 hr, and then plated onto gold electrodes for ECIS measurement. 24 hours after replating, the ECs were challenged with PM (100 μg/ml) and changes in TER were measured with ECIS. *p < 0.05 compared to PM-challenged group." } ]

[ { "from": "human", "value": "Characterize the image using a well-detailed description\n<image>" }, { "from": "gpt", "value": "Cranial musculoskeletal system of Elops lacerta, MZUSP 84787; left lateral view; nasal, antorbital, infraorbital series, and ventral portion of extrascapular removed. a Superficial musculature. b Deeper musculature; posterior portions of maxilla and supramaxillas, dorsal portion of pars temporalis of levator arcus palatini, and dorsoposterior portions of preopercle and segmentum facialis of adductor mandibulae additionally removed. Arrow indicates dilatator process of opercle. Abbreviations: AM, adductor mandibulae; CHD, constrictor hyoideus dorsalis; CMD, constrictor mandibularis dorsalis. Color code: Salmon, muscle; sand, bone; blue, cartilage; white, tendon or ligament (regular connective tissue); dark grey, irregular connective tissue" } ]

[ { "from": "human", "value": "Clarify the contents of the displayed image with great detail\n<image>" }, { "from": "gpt", "value": "Localization of the junctional components at AJs and TJs. (A) The signals for the AJ or TJ proteins are shown as indicated in each panel. Colon sections obtained from afadin cKO mouse littermates were immunostained with antibodies against the proteins as indicated. Scale bars: 30 μm. (B) Localization patterns of the actin cytoskeleton in the colon epithelia are compared between the control and afadin cKO mice. Scale bars: 200 μm." } ]

[ { "from": "human", "value": "Explain the various aspects of the image before you\n<image>" }, { "from": "gpt", "value": "Examples of slice selection. Upper random examples and lower largest outliers, for 6 different patients. Red line: manually selected slice, Blue line: automatically selected slice. The bottom left and bottom middle example were incorrectly selected in the automatic analysis, which identified slices at L2 and L1 level, respectively. The bottom right example was incorrectly selected by the manual method and is correctly identified by the automatic analysis." } ]

[ { "from": "human", "value": "Render a clear and concise summary of the photo.\n<image>" }, { "from": "gpt", "value": "Glaucoma progression analysis (GPA) for the patient's left eye." } ]

[ { "from": "human", "value": "Offer a succinct explanation of the picture presented.\n<image>" }, { "from": "gpt", "value": "Measurement with Vernier's caliper" } ]

[ { "from": "human", "value": "Clarify the contents of the displayed image with great detail\n<image>" }, { "from": "gpt", "value": "Overexpression of GADD45A does not promote demethylation of a methylated pOct4-EGFP plasmid. A. Schematic diagram of the pOct4-EGFP plasmid (not to scale). The 2.4 kb Oct4 promoter sequences are upstream of the EGFP gene. DE is the distal element, PE is the proximal element, and P is the minimal promoter of Oct4. This plasmid was methylated in vitro with HpaII or HpaII + HhaI. Black circles indicate HpaII sites and black triangles indicate HhaI sites. B. Confirmation of the methylation status of the six HpaII sites (black circles in panel A) by sodium bisulfite sequencing. Closed circles are methylated sites and open circles are unmethylated sites. C. Southern blot assay of plasmids recovered from HEK293 cells after transient transfection of the methylated and unmethylated pOct4-EGFP plasmid with and without GADD45A overexpression. The plasmids were digested with HincII and HpaII after in vitro methylation of the plasmid with HpaII (left panel) or HpaII and HhaI (right panel) prior to transfection. Digestion of the recovered methylated plasmid with HpaII does not lead to cleavage indicating that demethylation did not occur in HEK293 cells that overexpress GADD45A. D. Confirmation of GADD45A expression in transfected HEK293 cells by Western blotting with anti-GADD45A antibody. Beta-tubulin was probed as a loading control." } ]

[ { "from": "human", "value": "Characterize the image using a well-detailed description\n<image>" }, { "from": "gpt", "value": "The expression of Bax and Bcl-2 in HK-2 cells. The mRNA expression of Bax and Bcl-2 in HK-2 cells (a). P (compared with the control): N>0.05, ∗<0.05, ∗∗<0.01. P (intergroup comparison): n>0.05, &<0.05. The protein expression of Bax and Bcl-2 in HK-2 cells detected by simple Western assays (b). A: the control group, B: the melittin group, C: the TNF-α group, D: the melittin+TNF-α group. P (compared with the control): N>0.05, ∗<0.05, ∗∗<0.01. P (intergroup comparison): n>0.05. The protein expression of Bax and Bcl-2 in HK-2 cells detected by IF stains (c). IF: immunofluorescent. Magnification×200. P (compared with the control): ∗∗<0.01, ∗∗∗<0.001." } ]

[ { "from": "human", "value": "Offer a thorough analysis of the image\n<image>" }, { "from": "gpt", "value": "Selected peptides from skeletal muscle (SM) and central nervous system (CNS) abundant cysts proteins. A) Proteins predicted with high antigenic and/or epitope content. B) Ten proteins were selected: four SM (green) and four CNS (blue) abundant proteins. Two constitutive proteins were also chosen. One peptide was then selected for each protein." } ]

[ { "from": "human", "value": "Write a terse but informative summary of the picture.\n<image>" }, { "from": "gpt", "value": "Classification of polyphenols and their biological sources." } ]

[ { "from": "human", "value": "Clarify the contents of the displayed image with great detail\n<image>" }, { "from": "gpt", "value": "The relative amounts of metabolites (A) and lipids (B) at the E1 and E3 stages, using three V × V siblings susceptible to mealiness (<30% of juice content) from a peach segregating population. Each column represents the average of five biological replicates, and the bars are the standard deviations. Green boxes show the E1 stage (harvest), and red boxes show the E3 stage (harvest + 21 days at 0 °C). Statistical analysis was performed by using t-tests with p < 0.05." } ]

[ { "from": "human", "value": "Describe the image concisely.\n<image>" }, { "from": "gpt", "value": "Histogram showing the age of the participants according to age groups and sex (n = 314)" } ]

[ { "from": "human", "value": "Examine the image closely and share its details\n<image>" }, { "from": "gpt", "value": "Representative integrative genomics viewer (IGV) screenshots of two cases with HMGA2-NCOR2 gene fusion. A Case 1 with HMGA2 exon 3—NCOR2 exon 16 gene fusion. Note both split reads and paired reads at the 3′-border of HMGA2 exon 3 (left) corresponding to the 5′-end of NCOR2 exon 16 (right). B Case 2 with HMGA2 exon 3—NCOR2 exon 20 gene fusion. Note both split reads and paired reads at the 3′-border of HMGA2 exon 3 (left) corresponding to the 5′-end of NCOR2 exon 20 (right)." } ]

[ { "from": "human", "value": "Share a comprehensive rundown of the presented image\n<image>" }, { "from": "gpt", "value": "Result of artificial neural network by comparing predicted value against measured weight by using (A) 6 variables and (B) all 19 variables with (left) with training data, and (right) with test data." } ]

[ { "from": "human", "value": "Provide a detailed description of the given image\n<image>" }, { "from": "gpt", "value": "Schematic structure of the mathematical model. The area within the dotted line contains the contributions to the measured PET signal. The dashed line indicates the border between vascular and interstitial space. On the left hand side of the dashed line a two-compartment plasma PK model characterizes systemic PK. The subscript j can be either l (labeled) referring to 89Zr-mAb or u (unlabeled) referring to mAb" } ]

[ { "from": "human", "value": "Give a short and clear explanation of the subsequent image.\n<image>" }, { "from": "gpt", "value": "Permeation profiles of five NE formulations in comparison with control" } ]

[ { "from": "human", "value": "Share a concise interpretation of the image provided.\n<image>" }, { "from": "gpt", "value": "(A–C) are the visible-light photodegradation of RhB, MB, and theophylline, respectively. (D–F) are the recycling experiments of RhB, MB, and theophylline, respectively." } ]

[ { "from": "human", "value": "Write a terse but informative summary of the picture.\n<image>" }, { "from": "gpt", "value": "External environment conditions during the simulation time." } ]

[ { "from": "human", "value": "Describe the image concisely.\n<image>" }, { "from": "gpt", "value": "Results of the meta-analysis of the topographic cylinder." } ]

[ { "from": "human", "value": "Describe the following image in detail\n<image>" }, { "from": "gpt", "value": "Modulation of the P2 component in each syllable and condition. A. Grand average ERPs for words with initial, medial, and final stress-conditions shown separately at a midline (Cz) electrode position (upper left side). A very similar morphology of the P2 component for initial and final stress conditions can be observed in the topographical maps (upper right side). The difference waveform of the mean amplitude at the corresponding P2 time-window is shown for the first and third syllable for the initial and final stress condition, respectively: 1. initial stress (initial stress minus final stress conditions); 2. final stress (final stress minus initial stress conditions). Isovoltage mapping with spherical spline interpolation was used to depict the scalp distribution of the difference waveforms. No differences were found in the scalp distribution of both P2 components [Condition (first syllable, initial stress; last syllable, final stress) x electrode locations (29 electrodes): F(28, 364) = 1.01; P > 0.41]. B. The mean amplitude (standard error of the mean) for the peak-to-peak N1-P2 component at a midline (Cz) location for each syllable and condition." } ]

[ { "from": "human", "value": "Analyze the image in a comprehensive and detailed manner\n<image>" }, { "from": "gpt", "value": "Accumulation of PhAOC transcripts, AOC protein and activity in stem bases of cuttings of Petunia hybrida expressing PhAOC-RNAi. (a) Stem bases were collected from leafy cuttings of wild type (WT, green), two lines expressing the empty vector (pHell, blue) and three lines expressing a PhAOC-RNAi construct (red) at one hour after excision. Relative PhAOC transcript accumulation shown as 2−ΔCt values in relation to the reference gene PhRSP13 was determined by qRT-PCR. (b) AOC protein was detected immunocytologically in cross sections of stem bases using an antibody specific binding to AOC. Note the occurrence of AOC in plastids visible as distinct dots within cells (inset in WT). Bars represent 100 μm and 25 μm in overviews and inset, respectively. (c) AOC enzyme activity is given as nmol g−1 FW of enzymatically formed OPDA. Each value is represented by the mean of eight independent biological replicates ± SE. Different letters within each graph designate statistically different values (one-way-ANOVA with Tukey’s HSD test, P < 0.05)" } ]

[ { "from": "human", "value": "Analyze the image in a comprehensive and detailed manner\n<image>" }, { "from": "gpt", "value": "Effect of periodicity, P, of the narrow groove plasmonic nanogratings on the (a–e) reflectance versus wavelength curves for the nanograting. (f) Shift in resonance wavelength as a function of varying concentration of hydrogen. Effect of periodicity on the reflectance spectra and shift in resonance wavelength is shown for the following values of ‘P’: (a) 100 nm, (b) 125 nm, (c) 150 nm, (d) 200, (e) 250 nm. In all the cases above groove gap, G = 10 nm, height, H = 250 nm and thickness, t = 4 nm were taken." } ]

[ { "from": "human", "value": "Offer a succinct explanation of the picture presented.\n<image>" }, { "from": "gpt", "value": "Posterior transverse view of the elbow with hemarthrosis (triangle)." } ]

[ { "from": "human", "value": "Clarify the contents of the displayed image with great detail\n<image>" }, { "from": "gpt", "value": "Predictive model of Anopheles larval habitat presence. The top two panels show the output of the predictive model as the probability (P) of a larval habitat being present in each 20 by 20 m pixel. The bottom two panels show the probabilities converted to either “present” (i.e. at least one larval habitat is expected to be present in the 20 by 20 m pixel) or “absent”, using a threshold of P = 0.020. Maps on the right show close-up views of the maps on the left" } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "Effects of CT45 expression on self-renewal in endometrial cancer cell lines. To investigate of CT45 expression, stable knockdown of CT45 of IK-YBX2 cells and CT45 gene-rescued cells of them were established. (a) The level of CT45 expression of established cells was investigated by Western blotting. shRNAs significantly reduced the expression of CT45 (lane 3, 4 vs lane 5, 6), and the proteins were rescued using relevant gene expression (lane 7, 8 vs lane 9, 10). Full length blots are shown in Supplementary Fig. S5d online. (b) The serial spheres formation assays were performed using two different clones of IK-YBX2-shCT45 and IK-YBX2-sh control cells respectively. Representative spheres generated from a single cell after 14 days in non-adherent culture were shown (scale bar 150 μm). (c) Sphere formation was counted at passage (P) 1 to 5. The serial sphere forming capacity, was reduced in IK-YBX2-shCT45 cells compared with in IK-YBX2-sh control cells (**p < 0.01, *p < 0.05). (d) To exclude off target effect of shRNA, we also established cells which were rescued CT45 expression by introduction of CT45 cDNA into each clone of CT45 knocked down cells. Upon serial passage from P1-P4, IK-YBX2-shCT45-mock cells generated few tumorsphere during passages. Conversely, sphere formation efficiency was increased in IK-YBX2-shCT45-CT45 rescued cells and a significant increase was observed in of IK-YBX2 C3-shCT45-CT45 rescued cells (*p < 0.05). Data represent the means ± SD from three independent experiments." } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "Standard effect size (ses) of phylogenetic diversity (ses.PD), mean nearest taxon distance (ses.MNTD) and mean pairwise distance (ses.MPD) of Nematoda, Genus-assigned, Unassigned, Acantholaimus, Desmoscolex and Halalaimus ASVs in the BGR.PA, BGR.RA, IOM.C, GSR, IFREMER and APEI3 areas. Symbols represent outcome of the relevant t-test: squares, asterisk indicate statistically significant clustering and random phylogenetic structure, respectively." } ]

[ { "from": "human", "value": "Write a terse but informative summary of the picture.\n<image>" }, { "from": "gpt", "value": "Mind map of the research." } ]

[ { "from": "human", "value": "Describe the image concisely.\n<image>" }, { "from": "gpt", "value": "The biosynthesis and metabolism of (R)-salsolinol and N-methyl-(R)-salsolinol [45]." } ]

[ { "from": "human", "value": "Write an exhaustive depiction of the given image\n<image>" }, { "from": "gpt", "value": "Plasma concentrations of myeloperoxidase (A), MMP-9 (B), CXCL8 (C), GM-CSF (D) and VEGF-A (E) in asthma patients (red bars) and healthy controls (white bars) were measured by ELISA. The results were expressed as mean ± SEM. *p < 0.05; **p < 0.01; *** p < 0.005. Student’s t test or Mann–Whitney U test according to the parametric or nonparametric distribution of the variables" } ]

[ { "from": "human", "value": "Render a clear and concise summary of the photo.\n<image>" }, { "from": "gpt", "value": "C-T-D network of active pyrazine compounds in Maotai liquor" } ]

[ { "from": "human", "value": "Give a short and clear explanation of the subsequent image.\n<image>" }, { "from": "gpt", "value": "The six steps of Intervention Mapping [10]" } ]

[ { "from": "human", "value": "Render a clear and concise summary of the photo.\n<image>" }, { "from": "gpt", "value": "Localization of the PDGF-C gene on chromosome 4q32.1." } ]

[ { "from": "human", "value": "Give a short and clear explanation of the subsequent image.\n<image>" }, { "from": "gpt", "value": "Recovery influence by the amount of Fe3O4@SiO2-NH2-COP (a), by pH (b), by extraction time (c), and by extraction volume (d). The concentrations of the analytes were 5.0 μg·L−1." } ]

[ { "from": "human", "value": "Write a terse but informative summary of the picture.\n<image>" }, { "from": "gpt", "value": "It depicts average levels of self-efficacy across the three groups before and after the intervention." } ]

[ { "from": "human", "value": "Walk through the important details of the image\n<image>" }, { "from": "gpt", "value": "CO degradation by Desulfofundulus australicus strain AB33T with (A) 10%, (B) 20%, and (C) 40% of CO, Ptotal = 170 kPa. Open symbols: medium without sulfate; filled symbols: medium with sulfate (≈ 20 mM). The bars indicate maximum and minimum values measured (n = 3)." } ]

[ { "from": "human", "value": "Share a concise interpretation of the image provided.\n<image>" }, { "from": "gpt", "value": "Two Teflon beakers used to measure the absorbed power of DDI water for (A) vertical and (B) horizontal orientations." } ]

[ { "from": "human", "value": "Summarize the visual content of the image.\n<image>" }, { "from": "gpt", "value": "Model of a carbon nano-peapod with C70 fullerene encapsulated within a single-walled (10, 10) carbon nanotube." } ]

[ { "from": "human", "value": "Examine the image closely and share its details\n<image>" }, { "from": "gpt", "value": "Effect of AgRPS3aE overexpression on salt tolerance in transgenic Arabidopsis. (A) Northern blotting analysis of three independent T3 generation transgenic Arabidopsis lines; (B) The performance comparison of T3 and VC3 Arabidopsis seedlings stressed by 50 mM NaCl for 15 days; (C) The response bar graph for root length was calculated to illustrate the salt tolerance of AgRPS3aE transgenic Arabidopsis. The data presented are the means ± SD of three replications. ** indicates a significant difference at p < 0.01." } ]

[ { "from": "human", "value": "Write an exhaustive depiction of the given image\n<image>" }, { "from": "gpt", "value": "Functional prediction and prognostic value of upregulated metabolic enzymes in poorly-differentiated HCC (A) 135 upregulated metabolic genes and (B) 22 rate limiting enzymes (RLEs) in the 135 metabolic genes were mainly enriched in pyrimidine metabolism performed in KEGG pathway analysis. (C) Upregulated expression of 22 RLEs were associated with stemness gene signatures by GSEA. (D) Sum expression intensity of 22 upregulated RLEs is higher in poorly-differentiated HCC than that of well-differentiated HCC in TCGA-LIHC. (E) Higher expression of 22 upregulated RLEs is associated with poor survival of HCC patients in TCGA-LIHC. p value was calculated based on Mantel-Cox test and Gehan-Breslow-Wilcoxon test. ***p<0.0001 performed by two-tailed Student's t-test." } ]

[ { "from": "human", "value": "Provide a detailed description of the given image\n<image>" }, { "from": "gpt", "value": "Effect of different amounts of NaIO3 on yield of BPCAs. Reaction conditions: lignite (0.4 g), RuCl3 (40 mg), CCl4 (20 mL), CH3CN (20 mL), H2O (30 mL), NaIO4 (8 g), 35 °C, 6 h." } ]

[ { "from": "human", "value": "Give an elaborate explanation of the image you see\n<image>" }, { "from": "gpt", "value": "Accuracy assessment of the unified E-value (E ). The accuracy of the computed E s is evaluated by plotting the E[E ] versus rank. The E[E ]s at a given rank were obtained by averaging over all the computed E s from sample numbers 1–81 having the same rank. The curve made of red circles displayed in panels (a), (b), (c) are the curves of E[E ]s for microbial identification performed at the genus, species, and strain level. In each panel, the = line, corresponding to the theoretical line, together with the two dashed lines, = 3 and = /3, provide a visual guide regarding the accuracy of the computed E[E ] curves" } ]

[ { "from": "human", "value": "Walk through the important details of the image\n<image>" }, { "from": "gpt", "value": "IL-1β-induced IL-6 (A) and IL-11 (B) expression is attenuated by genetic deletion and pharmacological inhibition of TRPA1 in murine chondrocytes. Chondrocytes were obtained from TRPA1 deficient (knockout, KO) mice and corresponding wild-type (WT) mice. The chondrocytes were cultured with IL-1β (100 pg/mL) alone, or together with the selective TRPA1 antagonist HC-030031 (100 μM) or TCS 5861528 (100 μM) for 24 h and thereafter total RNA was extracted. IL-6 and IL-11 mRNA levels were measured with qRT-PCR and normalized against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA levels. The results are expressed as fold change in comparison to the control samples of each genotype. WT n = 13 (TCS treatment n = 10), KO n = 15. Results are expressed as mean + SEM. One-way ANOVA followed by Bonferroni post-test was performed; *** p < 0.001. (Adapted from the doctoral dissertation of the first author [28])." } ]

[ { "from": "human", "value": "Create a compact narrative representing the image presented.\n<image>" }, { "from": "gpt", "value": "Approximate onset time of post-injection delirium/sedation syndrome events." } ]

[ { "from": "human", "value": "Illustrate the image through a descriptive explanation\n<image>" }, { "from": "gpt", "value": "PLS-DA of sera samples from male and female mice with pancreatic tumors. (A) PLS-DA scores plot calculated using the first two principal components. The blue points indicate the control mice and the red points indicate the study mice. (B) Plot of R2Y and Q2 for the first three principal components. The green bars indicate the accumulated R2Y values and the blue bars indicated the accumulated Q2 values." } ]

[ { "from": "human", "value": "Narrate the contents of the image with precision\n<image>" }, { "from": "gpt", "value": "Scatterplots of the different measures of the average colour value of butterfly and dragonfly assemblages versus our thermal component 1. This component is a variable that summarizes the thermal environment within each grid from cool (low values) to warm (high values). Note that for all measures of colour value as well as for the two groups of insects, the colour lightness of assemblages is consistently correlated to the thermal environment. (a) Average colour values across species on a scale from 0 (black) to 255 (white). (b) Average phylogenetic component, that is, the predicted colour value of each species based on the phylogeny. (c) Average specific component, that is, the deviation from the colour value expected by the phylogeny. Parameters are from univariate regression models weighted with the number of species in each grid cell (butterflies: n=1,825; dragonflies: n=1,845). The inserted histograms are represented as lines in b and c show the distribution of regression slopes (β) calculated for 1,000 alternative phylogenetic trees. These regressions were all highly significant (t-test, P<0.001) with positive slopes throughout, indicating that the positive relationship between colour lightness and the thermal environment is robust to uncertainties in the phylogenetic trees." } ]

[ { "from": "human", "value": "Describe the following image in detail\n<image>" }, { "from": "gpt", "value": "Number of non-native disulfide-bond-containing isomers in any nS ensemble far exceeds the number of native disulfide-bond-containing species. The presence of a strong oxidizing agent rapidly oxidizes unstructured species, such as the 3SNNU species, to 4S prior to it being able to reshuffle to 3SNU, compromising the formation of N." } ]

[ { "from": "human", "value": "Present a compact description of the photo’s key features.\n<image>" }, { "from": "gpt", "value": "Forest plot depicting the association between smoking and need for colectomy in patients with ulcerative colitis, comparing current to never smokers and former to never smokers" } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "Cellularity in bronchoalveolar lavage fluid and lung before and after bleomycin challenge at day 7 in wildtype vs. gp91phox-/- and MMP12-gp91phox double knockout mice. Airways were lavaged and cells counted by a Z1 particle counter from Beckman Coulter. Lung lobes were excised after thorough exsanguination of the mouse by infra-orbital bleeding after ligation of the superior vena cava. Minced parenchyma was digested with dispase and the resultant cell suspension, filtered of cell debris, was stained with Hoechst dye, and cellularity assessed. *P < 0.05 compared to control, averaged from three independent experiments. FACsans and flow sorts were done in triplicate per sample. Samples were counted in duplicate tubes per sample for each data point (n = 4 to 5 per group). Mortality was less than 2%. Abbreviations: WS, saline-treated wildtype mice; WB, wildtype mice treated with bleomycin; NOXS, saline-treated gp91phox null; NOXB, bleomycin-treated gp91phox null; DKOS, saline treated MMP12- gp91phox double knockout; DKOB, bleomycin-treated MMP12- gp91phox double knockout." } ]

[ { "from": "human", "value": "Provide a detailed description of the given image\n<image>" }, { "from": "gpt", "value": "Scatterplot of 90 microhaplotypes by In and average Ae for 79 populations (79p). 6 MHs with highest Ae values in all 6 biogeograhic regions (cf Table 4) are shown as patterned circles" } ]

[ { "from": "human", "value": "Give an elaborate explanation of the image you see\n<image>" }, { "from": "gpt", "value": "Ki-67 immunohistochemical analysis of the EVPOME received metabolic challenges compared with the standard protocol. Representative images of Ki-67 immunohistochemistry of the EVPOMEs. Ki-67-positive cells are mainly present in the basal layer. The number of Ki-67 positive cells was remarkably fewer when cells received metabolic challenges before development of the EVPOMEs. (A) Standard protocol, (B) metabolic challenge of 5×PBS, (C) metabolic challenge of 20×PBS, (D) metabolic challenge of 4dNoF, (E) metabolic challenge of 7dNoF. Original magnification × 40. Scale bar = 100 μm." } ]

[ { "from": "human", "value": "Provide a brief description of the given image.\n<image>" }, { "from": "gpt", "value": "Change of center frequency." } ]

[ { "from": "human", "value": "Relay a brief, clear account of the picture shown.\n<image>" }, { "from": "gpt", "value": "Induration of stoma area associated with leakage through the stoma." } ]

[ { "from": "human", "value": "Break down the elements of the image in a detailed manner\n<image>" }, { "from": "gpt", "value": "Expression and activation of HER4 in brain-metastatic breast cancers and brain metastases. (A) Chi-square analysis of cyto.HER4, nu.4ICD and m.pHER4 in major breast cancer subtypes. *p < 0.05; ***p < 0.001; ****p < 0.0001. (B) Changes in cyto.HER4, nu.4ICD and m.pHER4 in individual matched cases, separated according to major breast cancer subtypes. Chi-square p-values indicate significant differences before and after metastasis to the brain. (C) Tile plot showing overall numbers of br.MBC and BrM co-expressing pHER receptors (columns are individual tumours). (D) Representative IHC analysis of samples from a patient whose HER2+ breast cancer recurred in the brain 7 years after diagnosis and treatment with HER2-targeted therapy. This case exemplifies concomitant induction of pHER2, pHER3 and pHER4. BrM, brain metastases; br.MBC, brain metastatic breast cancers; cyto.HER4, cytoplasmic HER4; HER4, human epidermal growth factor receptor-4; IC-NST, invasive carcinoma, no special type; IHC, immunohistochemistry; LumA/B, luminal A/B; m.pHER4, membrane-pHER4; nu.4ICD, nuclear HER4-ICD." } ]

[ { "from": "human", "value": "Offer a thorough analysis of the image\n<image>" }, { "from": "gpt", "value": "Relationship between effective population size (Ne) and normalized inferred iAI and normalized oAI candidates. For each epoch, the mean Ne was computed using MSMC by Pagani et al. (2016). The mean Ne for 30–40 ka is plotted against (A) the number of windows detected as inferred iAI candidates in each population normalized by the sample size of the population in our data set and (B) the number of oAI candidates also normalized based on sample size. The best fitting linear model, its correlation, as well as the Spearman Rank Correlation test P-value is shown. Data for every epoch tested are shown for iAI in supplementary figure S25, Supplementary Material online and for other candidates in supplementary figure S26, Supplementary Material online. (C) Raw correlation of normalized iAI and oAI compared with Ne at different times. The combined P-value of the correlation across all time bins is significant with P = 0.000051 using Fisher’s method for combining P-values. The date of Neanderthal interbreeding is taken as ∼55 ka following Fu et al. (2014). Confidence intervals are calculated by bootstrap resampling." } ]

[ { "from": "human", "value": "Relay a brief, clear account of the picture shown.\n<image>" }, { "from": "gpt", "value": "Sensitivity analysis showing effect on composite outcome (New Onset Stroke + Mortality)." } ]

[ { "from": "human", "value": "Characterize the image using a well-detailed description\n<image>" }, { "from": "gpt", "value": "Box–whisker plots showing differences in the number of piglets born per litter among sows hierarchically grouped according to the mean ovarian follicle diameter at weaning: Small (0.20–0.30 cm), medium (0.31–0.39 cm), or large (0.40–1.00 cm) ovarian follicles at weaning. Boxes enclose the 25th and 75th percentiles; the line is the median; and the whiskers extend to the 5th and 95th percentiles. Grey dots show outliers. a and b indicate significant differences (p < 0.05)." } ]

[ { "from": "human", "value": "Render a clear and concise summary of the photo.\n<image>" }, { "from": "gpt", "value": "HILIC–HR–ESI–MS spectra of classes of phospholipids (PL) identified in negative ion mode as [M−H]−. These classes were (A) phosphatidylinositol (PI) and (B) phosphatidylglycerol (PG)." } ]

[ { "from": "human", "value": "Summarize the visual content of the image.\n<image>" }, { "from": "gpt", "value": "Frontal view of Camper’s plane (ala-tragus) and occlusal plane." } ]

[ { "from": "human", "value": "Create a compact narrative representing the image presented.\n<image>" }, { "from": "gpt", "value": "ROC of 5CV mapped by MSPEDTI on enzyme dataset." } ]

[ { "from": "human", "value": "Illustrate the image through a descriptive explanation\n<image>" }, { "from": "gpt", "value": "From our analysis, we found that the coherence score for our model on our dataset peaks at around 12 topics, as observed in the above chart. Beyond 12 topics, the improvement to the coherence score of the model is imperceptible and leads to loss of coherence between topics" } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "Pure-tone audiograms of the hearing-impaired listeners. Individual audiograms of Listeners 1 to 9 (from lowest to highest pure-tone average) are color coded according to the legend (color available online). The N2 standard audiogram (shown in black, Bisgaard et al., 2010) was used to derive the compression ratios, following the NAL-NL2 procedure (Keidser et al., 2011)." } ]

[ { "from": "human", "value": "Portray the image with a rich, descriptive narrative\n<image>" }, { "from": "gpt", "value": "Increased number of PD-1+ memory CD8+ T cells after IT in CD4+ T cell deficient mice. Control or CD4 deficient (depleted or knockout) C57BL/6 mice were treated with IT or PBS/rIgG (control) and PD-1 expression on memory T-cells was quantified by flow cytometric analysis 11 days after the initiation of IT. (a) Representative dot plots for PD-1+ gating on CD8+ CD44high cells in the spleens of CD4+ depletion model mice. Number of PD-1+ memory (CD44high) CD8+ T cells in spleens (b,d) and LNs+ (c,e) of IT or vehicle treated mice in CD4+ depletion (b,c) or CD4 knockout (d,e) models. Results are representative of two (CD4 knockout) or three (CD4 depletion) independent experiments with a minimum of three mice per group. (*P<.05, **P<.01, ***P<.001)." } ]

[ { "from": "human", "value": "Provide a detailed description of the given image\n<image>" }, { "from": "gpt", "value": "Correlation between PAEEdlw (physical activity energy expenditure) and PAEEdev during 15 free-living days. Scatter plots for PAEEdlw (x-axis) and PAEEdev (y-axis) during 15 free-living days. Upper and lower values for r and ρ resulting from Pearson and Spearman correlation tests, respectively, are shown. n.s.: nonsignificant." } ]

[ { "from": "human", "value": "Give an elaborate explanation of the image you see\n<image>" }, { "from": "gpt", "value": "The biomechanical knee joint test bench [17] with a knee specimen fixed at 30° of flexion. Anterior tibial translation force (red arrow) is induced by a pulley-and-weight system. A pnemuatic rotatory cylinder (a) is used to apply internal tibial torque (green arrow). The tibial weight is compensated in relation to the flexion angle by another pulley-and-weight system (blue arrow). Graft forces and tibiofemoral joint motion are measured by custom graft force measuring devices (incorparating a load cell) (b) and an optical 3D camera system (c) respectively" } ]

[ { "from": "human", "value": "Provide a brief description of the given image.\n<image>" }, { "from": "gpt", "value": "Molecular packing of (I) with hydrogen bonding shown as dashed lines." } ]

[ { "from": "human", "value": "Narrate the contents of the image with precision\n<image>" }, { "from": "gpt", "value": "Diagram with causal relationships among total effective xenoestrogen burden of α (TEXB-α) and β (TEXB-β) fractions, breast cancer, and unmeasured exposures to xenoestrogens and endogenous hormones. The causal path from TEXB-α to breast cancer X→Y is confounded by the indirect path X←U 1→Z→Y, which can be blocked by adjusting for TEXB-β. However, conditioning on TEXB-β unblocks the other indirect path X←U 1→Z←U 2→Y, thus resulting in selection bias." } ]

[ { "from": "human", "value": "Illustrate the image through a descriptive explanation\n<image>" }, { "from": "gpt", "value": "CARD10 and COPS8 are direct miR-146a targets. () mRNA expression of the two new miR-146a targets, CARD10 and COPS8, and IRAK1, a known miR_146a target, was determined by qPCR in control- and miR-146a-transfected SNU638 cells. Expression of each transcript is shown relative to the expression in control-transfected cells. CARD10, COPS8 and IRAK1 expression was down-regulated following miR-146a over-expression. Data are shown as mean ± S.D. of four biological replicates. * = P < 0.05, ** = P < 0.01, *** = P < 0.001. () miR-146a transfection also reduced the protein levels of CARD10, COPS8 and IRAK1 in miR-146a-transfected SNU638 cells. The expression of each protein is shown relative to the expression in control-transfected cells. Bands were quantified relative to β-actin. Data are shown as mean ± S.D. of four biological replicates. * = P < 0.05, ** = P < 0.01, *** = P < 0.001. () Verification of direct and functional target binding using luciferase constructs holding WT 3’UTRs and mutated (Mut) 3’UTRs (the central 4 nucleotide were replaced in miR-146a binding site) for CARD10, COPS8 and IRAK1. Constructs containing either WT or mutated (Mut) 3’UTRs downstream to the firefly luciferase gene were co-transfected into HEK293 cells together with Renilla luciferase control plasmid and either miR-146a or control (siGlo). Left, luciferase activity is given relative to activity in control-transfected cells. miR-146a reduced luciferase activity of constructs with WT CARD10, COPS8 and IRAK1 3’UTRs. Right, sequence alignments of miR-146a and potential WT and Mut 3’UTR target sites are shown, mutated bases are shown in red. Data are shown as mean ± S.D. often biological replicates. *** = P < 0.001. Sequence alignments are adapted from DIANALab (2009)." } ]

[ { "from": "human", "value": "Narrate the contents of the image with precision\n<image>" }, { "from": "gpt", "value": "Effective orifice area computed using shear layer detection method (JSLD). Effective orifice area computed using jet shear layer detection method (JSLD). Panel (A) shows flow streamlines through an orifice (geometric orifice area [GOA] = 1.66 cm2 and effective orifice area [EOA] = 1.00 cm2). Dashed line represents a plane crossing the vena contracta (≈ 10 mm from the orifice). Panel (B) shows normalized velocity map at the vena contracta. Panel (C) shows AST shear layers computed from (B), JSLD identifies the inflexion points from the velocity profile corresponding to noise production, shear layers from vorticity and separation regions at the vena contracta position lead to EOA estimation (blue circle with transparency). Panel (D) shows the correspondence between normalized velocity profiles and normalized AST profiles, gray lines indicate the corresponding voxel on EOA identified shear layer" } ]

[ { "from": "human", "value": "Render a clear and concise summary of the photo.\n<image>" }, { "from": "gpt", "value": "View of the title molecule. Displacement ellipsoids are drawn at the 30% probability level. H atoms are represented by circles of arbitrary size." } ]

[ { "from": "human", "value": "Offer a thorough analysis of the image\n<image>" }, { "from": "gpt", "value": "Phylogenetic analysis of purine nucleoside phosphorylases (PNPs). AhPNP (GenBank:WP_049045821) from Aeromonas hydrophila; BcPNP (GenBank:WP_098273438) from Bacillus cereus; BhPNP (GenBank: WP_010897696) from Bacillus halodurans; BtPNP (GenBank: AAX46392) from Bos taurus; Bs3PNP (GenBank: WP_015714207) from Bacillus subtilis; Bs6PNP (GenBank: WP_044428467) from Bacillus subtilis; DrPNP (GenBank: NP_998476) from Danio rerio; EcPNP (GenBank: AAN83888) from Escherichia coli; FhPNP (GenBank: XP_012736771) from Fundulus heteroclitus; GsPNP (GenBank: WP_053414649) from Geobacillus stearothermophilus; HaPNP (GenBank:KFP95377) from Haliaeetus albicilla; HsPNP (GenBank: NP_000261) from Homo sapiens; MmPNP (GenBank: NP_038660) from Mus musculus; SsPNP (GenBank: WP_009988635) from Sulfolobus solfataricus; StPNP (GenBank: WP_011681178) from Streptococcus thermophilus; TtPNP (GenBank: WP_096412123) from Thermus thermophilus; and XtPNP (GenBank: NP_001006720) from Xenopus tropicalis." } ]