Question: Working on new project, which requires- 1st port is for HOST function with Type A connector and 2nd is for Device function with Type B connector & not support USB host port and device port at the same time. how that can be design with PIC controller.

Answer: 
Your problem isn't so much the scope probes as the breadboard.

The breadboard adds series inductance (as though you had installed coils in series with the SPI signals) and parallel capacitance (as though you had put small capacitors from the SPI signals to ground.) This will slow the rising and falling edges, making it more difficult to detect level changes. The inductance can also cause ringing - there can be false level transitions from the ringing.

Attach your probes directly to the SPI signals, right at the point where the wires connect to one of your boards.

Use 10X probes if you have them - they are less of a load on the signal lines.

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[SPI](https://en.wikipedia.org/wiki/Serial_Peripheral_Interface) uses relatively fast signals, and isn't intended to be used between circuit boards.

It is intended for use on a single board with short connections.

The wires between the boards are probably making your SPI signals "unhappy," never mind the breadboard and the additional load of the oscilloscope.

1. Use short wires.
2. Keep the circuit neat.
3. Use straight wires rather than letting things twist around and get tangled.

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Put the breadboard down and back away slowly. Nobody has to be injured here.
The thing to look out for is the parasitic capacitance you create on the communication lines.

* I would suggest to keep the lines short and maybe try to bypass the breadboard.
* Does it work with the breadboard without the scope connected ?
* Do you see some signal on the scope ?

Also how is your oscilloscope grounded ? Make sure you clip the ground probe somewhere. If your device under test is floating, you can't rely on the earth reference.