Question: Working on new project, which requires- 1st port is for HOST function with Type A connector and 2nd is for Device function with Type B connector & not support USB host port and device port at the same time. how that can be design with PIC controller.

Answer: 
The thing to look out for is the parasitic capacitance you create on the communication lines.

* I would suggest to keep the lines short and maybe try to bypass the breadboard.
* Does it work with the breadboard without the scope connected ?
* Do you see some signal on the scope ?

Also how is your oscilloscope grounded ? Make sure you clip the ground probe somewhere. If your device under test is floating, you can't rely on the earth reference.
While other answers are fairly correct, in my experience the big problem with breadboards, especially the cheap ones, is that as soon as you use wires of different sizes, it spreads apart the breadboard spring plates and causes bad contacting.

My guess is when you insert your scope wire into the breadboard, this is exactly what happens. Try to probe a different way, use a higher quality breadboard, or solder your circuit on a test PCB (they are cheap these days).

Personally, I mostly stopped working with breadboard altogether because of these issues, and as the cost of PCBs has become so low that you can just make a PCB for 5$, and there are fewer and fewer DIP components.