Question: Did Peter Parker take part in, or have knowledge of what happened to the rest of the radioactive spiders? Were they disposed of or are they are still hanging around in a lab somewhere?

Answer: 
**Amazing Spider-Man** (Cinematic Universe)
-------------------------------------------

*When the question indicated spiders (plural), my assumption was the recent Amazing Spider-Man movie which featured dozens of transgenic spiders weaving for Norman Osborn. In most versions of the canon story, there was only one spider, which was irradiated.*

As far as I can tell, the spiders are still at Osborn's laboratory being used for whatever insidious and yet financially profitable scheme they were commissioned for. In this particular instance, I don't believe they were strictly radioactive, let's call them [transgenic](http://en.wikipedia.org/wiki/Transgene) spiders.

Their bite, while painful, does not appear to offer superhuman abilities to anyone besides's Peter Parker indicating, perhaps a greater purpose or genetically engineered process being held within or released through the bite of the spider.

Though it was not clearly stated the radioactive spiders would not have had the same effect on anyone else that they had on Peter. It was meant to be revealed during the course of the movie that Parker's powers were not an accident as they appeared but something done intentionally.

To cover the whole conspiracy theory properly, you need to see: [Was the Untold Story Cut from the Amazing Spider-Man?](http://badassdigest.com/2012/07/05/was-the-untold-story-cut-from-the-amazing-spider-man/)

**Amazing Spider-Man** (Marvel Earth-616)
-----------------------------------------

If you are talking about the Marvel Earth-616, there were no known "other spiders" after the first one bit Peter Parker. It died from radiation poisoning soon after spreading its radiation-activated powers to an unwitting recipient.
If we're speaking about the spiders [plural] from the Sam Raimi trilogy, its somewhat unknown. It *Was* said that traits from various spiders were taken and recombined into "These 15 genetically altered super spiders" to which Mary Jane pointed out "There are only 14; one's missing," [not counting the one that escaped and bit Peter]. But after that school visit, I don't believe any further thought or mention was given to them, even to finding the one that escaped.

To be fair, these weren't "Radioactive" spiders, and the one that bit Pete just wondered off. Thinking about it, this is a *Severe* ball drop on Peter's part; presuming they were all genetically and structurally identical, **any one of those spiders** could then imbue someone *else* with Peter's powers! Not an ideal situation....