Choroid and Sclera cells from adult human eyes were separately dissociated and cultivated in the presence of epidermal growth factor and 10% fetal bovine serum. No retinal pigmented epithelial cells were detected. After cell growth, cells were transferred under conditions known to induce neuronal differentiation. Cells were plated on laminin in the presence of fibroblast growth factor-2 or brain-derived neurotrophic factor.