Materials All chemicals were from Sigma-Aldrich (Oakville, ON, Canada), and used without any additional purification. All organic solvents were from Caledon labs (Georgetown, ON, Canada). BcMag DEAE magnetic beads were from Bioclone Inc. (San Diego, CA). Desalting columns (illustra NAP-5) were from GE Life Sciences (Quebec, Canada). Amicon Ultra-0.5 centrifugal filters were from Fisher Scientific (Ontario, Canada). CdSxSe1-x/ZnS (core/shell) alloyed semiconductor nanoparticles with emission wavelengths of 525 nm, 575 nm and 630 nm were from Cytodiagnostics Inc. (Burlington, ON, Canada). Peptide sequences were from CanPeptide Inc (Montreal, QC, Canada): (6-Maleimidohexanoic acid) G(Aib)GHHHHHH – DNA sequences were from IDT DNA (Coralville, IA, USA): Probe DNA 5’- Thiol TTT TTT TTT TCT TAC TTC CAT GAT TTC TTT AAC – TAT GCC G– 3’ Displacer DNA 5’ AAA GAA ATC ATG GAA GTA AGT TTT TTT TTT – – (Proximal) Displacer DNA 5’ Thiol TTT TTT TTT TCG GCA TAG TTA AAG AAA TCA-3’ – – (Distal) Target DNA 5’ CCA CGG TGA TAT CGT CCA CCC AGG TGT TCG GCG – TGG TGT AGA GCA TTA CGC TGC GAT GGA TCC CGG CAT AGT TAA AGA AAT CAT GGA AGT AAG 3’ – Buffers: Borate buffer: 100 mM borate, pH 9.25 Tris-borate buffer (TB): 100 mM tris, 100 mM borate, pH 7.3 Phosphate buffered saline (PBS): 10 mM phosphate, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4
Materials All chemicals were from Sigma-Aldrich (Oakville, ON, Canada), and used without any additional purification. All organic solvents were from Caledon labs (Georgetown, ON, Canada). BcMag DEAE magnetic beads were from Bioclone Inc. (San Diego, CA). Desalting columns (illustra NAP-5) were from GE Life Sciences (Quebec, Canada). Amicon Ultra-0.5 centrifugal filters were from Fisher Scientific (Ontario, Canada). CdSxSe1-x/ZnS (core/shell) alloyed semiconductor nanoparticles with emission wavelengths of 525 nm, 575 nm and 630 nm were from Cytodiagnostics Inc. (Burlington, ON, Canada). Peptide sequences were from CanPeptide Inc (Montreal, QC, Canada): (6-Maleimidohexanoic acid) – G(Aib)GHHHHHH DNA sequences were from IDT DNA (Coralville, IA, USA): Probe DNA 5’- Thiol – TTT TTT TTT TCT TAC TTC CAT GAT TTC TTT AAC TAT GCC G– 3’ Displacer DNA (Proximal) 5’ – AAA GAA ATC ATG GAA GTA AGT TTT TTT TTT – Displacer DNA (Distal) 5’ – Thiol – TTT TTT TTT TCG GCA TAG TTA AAG AAA TCA-3’ Target DNA 5’ – CCA CGG TGA TAT CGT CCA CCC AGG TGT TCG GCG TGG TGT AGA GCA TTA CGC TGC GAT GGA TCC CGG CAT AGT TAA AGA AAT CAT GGA AGT AAG – 3’ Buffers: Borate buffer: 100 mM borate, pH 9.25 Tris-borate buffer (TB): 100 mM tris, 100 mM borate, pH 7.3 Phosphate buffered saline (PBS): 10 mM phosphate, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4
Au_QD%20interaction%20Supporting%20Information.docx