described in Ran et al. (2002). In brief, about 5 g of the frozen-dried sediment sample was treated with 10% HCl for 24 h to remove inorganic carbon, and then dried overnight at 105°C. The organic carbon contents were determined on an elemental analyzer (Vario EL III Elementar, Germany) with acetanilide used as external standard. Sample duplicates were processed and their mean values are reported in Table 1. E.coli and total coliform groups in seawater samples were measured using the membrane filtration method adopted by the Environmental Protection Department, the Government of the Hong Kong Special Administrative Region (). Sample extraction, clean-up, and analysis：Phenolic estrogens Phenolic estrogens in seawater, sediment and biota samples (Gatidou et al., 2010; Zhao et al., 2011) One litre of each of the seawater samples was filtered through a glass fibre filter (Whatman GF/F, 0.7 UK). Methanol was used to elute non-filterable particles on the μm, filter and combined with the filtered sample. For solid phase extraction (SPE), 100 each μL of 1 mg/L of 4-n-NP was added to each sample as an internal standard. SPE cartridge (Oasis HLB, 6 mL, 500 mg) was preconditioned with methanol / HPLC water (1:1, v/v). The filtered water samples passed through the SPE cartridges at 10 mL/min. The target compounds were eluted from the cartridges using methanol and dichloromethane. The extracts were dried and then re-dissolved in methanol. Target compounds in the sediment or tissue samples were extracted by ultrasonic-assisted solvent extraction. Five grams of the prepared samples were mixed with ethyl acetate (10 mL). The tube was ultrasonicated for 15 min and centrifuged at 1370 g for 10 min, and the supernatant was collected. The sediments were extracted two more times with 10 mL and 5 mL of ethyl acetate, respectively. The supernatants were combined and concentrated to about 1 mL on a rotary
described in Ran et al. (2002). In brief, about 5 g of the frozen-dried sediment sample was treated with 10% HCl for 24 h to remove inorganic carbon, and then dried overnight at 105°C. The organic carbon contents were determined on an elemental analyzer (Vario EL III Elementar, Germany) with acetanilide used as external standard. Sample duplicates were processed and their mean values are reported in Table 1. E.coli and total coliform groups in seawater samples were measured using the membrane filtration method adopted by the Environmental Protection Department, the Government of the Hong Kong Special Administrative Region (). Sample extraction, clean-up, and analysis：Phenolic estrogens Phenolic estrogens in seawater, sediment and biota samples (Gatidou et al., 2010; Zhao et al., 2011) One litre of each of the seawater samples was filtered through a glass fibre filter (Whatman GF/F, 0.7 μm, UK). Methanol was used to elute non-filterable particles on the filter and combined with the filtered sample. For solid phase extraction (SPE), 100 μL each of 1 mg/L of 4-n-NP was added to each sample as an internal standard. SPE cartridge (Oasis HLB, 6 mL, 500 mg) was preconditioned with methanol / HPLC water (1:1, v/v). The filtered water samples passed through the SPE cartridges at 10 mL/min. The target compounds were eluted from the cartridges using methanol and dichloromethane. The extracts were dried and then re-dissolved in methanol. Target compounds in the sediment or tissue samples were extracted by ultrasonic-assisted solvent extraction. Five grams of the prepared samples were mixed with ethyl acetate (10 mL). The tube was ultrasonicated for 15 min and centrifuged at 1370 g for 10 min, and the supernatant was collected. The sediments were extracted two more times with 10 mL and 5 mL of ethyl acetate, respectively. The supernatants were combined and concentrated to about 1 mL on a rotary
1-s2.0-S0269749116303554-mmc1.docx