Table S1. Characteristics of Sudan dyes. Log P Analytes Molecular structure Molar mass (g mol -1) pKa Sudan I 11.65 4.24 248.3 II 11.65 5.16 276.3 Sudan III 11.44 5.74 352.4 IV N.a 6.66 380.4 Sudan Red G 4.99 278.3 11.8 Sudan orange G 214.2 11.67 2.44 Table S2. Comparison between proposed method and other extraction methods for determination of Sudan dyes. Analysis Analytical method Analyte LOD (ng mL-1) LDR (ng mL−1) ER% PF time (min) Sudan I 28.8 50-1000 - - SFE–capillary liquid Sudan II 23.2 50-1000 - - 35 chromatography Sudan III 32.3 50-1000 - - Sudan IV 42.0 50-1000 - - Sudan I 7.7 27-2900 37 - Sudan II 8.3 29-2300 34 - OS-AALLME/ HPLC-UV Sudan III 12.1 40-2600 37 4.5 - Sudan IV 10.6 36-2800 33 - Orange G 84.8 280-3100 35 Sudan I 50 200-5000 - - Ultrasound-assisted Sudan II 60 200-5000 - - 20 LLE/HPLC-DAD Sudan III 70 200-5000 - - Sudan IV 100 500-5000 - - Sudan I 0.19 0.5-1000 - 147 Sudan II 0.39 1.0-1000 - 61 DSSBME-HPLC-UV ≥20 Sudan III 0.56 5.0-1000 - 35 Sudan IV 0.75 5.0-1000 - 38 I 0.09 1.0-1000 - 186 U-HF–LPME– Sudan II 0.31 1.0-1000 - 52 ≥40 HPLC–UV III 0.63 5.0-1000 - 38 Sudan IV 0.95 10.0-1000 - 37 Sudan I 12.8 20-20000 - - Sudan II 12.0 20-20000 - - ILE–HPLC/UV 32 Sudan III 11.2 20-20000 - - Sudan IV 13.2 20-20000 - -
Table S1. Characteristics of Sudan dyes. Analytes Molecular structure Molar mass (g mol -1) pKa Log P Sudan I 248.3 11.65 4.24 II 276.3 11.65 5.16 Sudan III 352.4 11.44 5.74 IV 380.4 N.a 6.66 Sudan Red G 278.3 11.8 4.99 Sudan orange G 214.2 11.67 2.44 Table S2. Comparison between proposed method and other extraction methods for determination of Sudan dyes. Analytical method Analyte LOD (ng mL-1) LDR (ng mL−1) ER% PF Analysis time (min) SFE–capillary liquid chromatography Sudan I Sudan II Sudan III Sudan IV 28.8 23.2 32.3 42.0 50-1000 50-1000 50-1000 50-1000 - - - - - - - - 35 OS-AALLME/ HPLC-UV Sudan I Sudan II Sudan III Sudan IV Orange G 7.7 8.3 12.1 10.6 84.8 27-2900 29-2300 40-2600 36-2800 280-3100 - - - - 37 34 37 33 35 4.5 Ultrasound-assisted LLE/HPLC-DAD Sudan I Sudan II Sudan III Sudan IV 50 60 70 100 200-5000 200-5000 200-5000 500-5000 - - - - - - - - 20 DSSBME-HPLC-UV Sudan I Sudan II Sudan III Sudan IV 0.19 0.39 0.56 0.75 0.5-1000 1.0-1000 5.0-1000 5.0-1000 - - - - 147 61 35 38 ≥20 U-HF–LPME– HPLC–UV I Sudan II III Sudan IV 0.09 0.31 0.63 0.95 1.0-1000 1.0-1000 5.0-1000 10.0-1000 - - - - 186 52 38 37 ≥40 ILE–HPLC/UV Sudan I Sudan II Sudan III Sudan IV 12.8 12.0 11.2 13.2 20-20000 20-20000 20-20000 20-20000 - - - - - - - - 32
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