of pBR322, part I (EMS mutagenesis) PGMG page 404 (chemical mutagenesis) Friday Nov 9: Visit to the Advanced Genetic Technology Center Nov 12: Lab 21 Analysis of northern blots; Random mutagenesis of pBR322, part II (DNA isolation); short Quiz Nov 14: Lab 22 Random chemical mutagenesis of pBR322, part III (E. coli transformation); Detection of epitope-tagged proteins by Western blot, part I (protein preparation) PGMG chapter 21 Interactome genome-wide studies on gene & protein interactions Friday Nov 16 – Nov 19: Lab 23 Random chemical mutagenesis of pBR322, part IV (replica plate); Detection of epitope-tagged proteins by Western blot transfer & PAP antibody presentation, part II Nov 21: Thanksgiving Break, academic holiday Friday Nov 23: Thanksgiving Break, academic holiday Random chemical mutagenesis of pBR322, part IV (replica plate results); Nov 26: Lab 24 Detection of epitope-tagged proteins by Western blot, part III (HRP & AP detection) PGMG 26 Nov 28: Lab 25 Detection of epitope-tagged proteins by Western blot, part IV (AP detection) Quiz Friday Nov 30 Visit to the Proteomics Core Facility Dec 3: Lab 26 Complete ongoing experiments, final lecture topics Dec 5: Lab 27 Lab cleanup, exam review Fri Dec 7 TBA Dec 12 Final exam, 1:00 PM, THM 109 – Homework Assignments & Plagiarism CUT and PASTING text from the internet or direct quotation from web sites or other sources, including you lab partner, is not allowed even if attributed.
of pBR322, part I (EMS mutagenesis) PGMG page 404 (chemical mutagenesis) Friday Nov 9: Visit to the Advanced Genetic Technology Center Nov 12: Lab 21 Analysis of northern blots; Random mutagenesis of pBR322, part II (DNA isolation); short Quiz Nov 14: Lab 22 Random chemical mutagenesis of pBR322, part III (E. coli transformation); Detection of epitope-tagged proteins by Western blot, part I (protein preparation) PGMG chapter 21 Friday Nov 16 Interactome – genome-wide studies on gene & protein interactions Nov 19: Lab 23 Random chemical mutagenesis of pBR322, part IV (replica plate); Detection of epitope-tagged proteins by Western blot transfer & PAP antibody presentation, part II Nov 21: Thanksgiving Break, academic holiday Friday Nov 23: Thanksgiving Break, academic holiday Nov 26: Lab 24 Random chemical mutagenesis of pBR322, part IV (replica plate results); Detection of epitope-tagged proteins by Western blot, part III (HRP & AP detection) PGMG 26 Nov 28: Lab 25 Detection of epitope-tagged proteins by Western blot, part IV (AP detection) Quiz Friday Nov 30 Visit to the Proteomics Core Facility Dec 3: Lab 26 Complete ongoing experiments, final lecture topics Dec 5: Lab 27 Lab cleanup, exam review Fri Dec 7 TBA Dec 12 – Final exam, 1:00 PM, THM 109 Homework Assignments & Plagiarism CUT and PASTING text from the internet or direct quotation from web sites or other sources, including you lab partner, is not allowed even if attributed.
BIO%20510%20revised%20full%20lab%20set_0.docx