helps in the removal of both transient and resident microorganisms that can cause disease or infection Part 2 Questions 1. Some microorganisms that are commonly found on the skin of the hands are: Gram-positive cocci: Gram-negative bacilli: sp., sp., Staphylococcus, Klebsiella-Enterobacter Acinetobacter sp., sp. and Yeast. is the only true Pseudomonas Proteus-providencia Staphylococcus aureus pathogenic organism included in the resident microflora group of skin, and some, but not all, individuals carry on their skin. The population of Staphylococcus aureus Staphylococcus far out numbers on healthy skin epidermidis S. aureus 2. The cultural medium used in the hand scrub experiment was veal infusion agar (VIA) pours in 50̊ C water bath. The plates sat until solid and were incubated to grow the organisms isolated from the hands for 48 hours at 37̊ C. 3. and yeasts can also grow in this medium. Escherichia coli, S. minus 4. I would expect that there will never be organisms if you continued scrubbing your ‘zero’ hands in this experiment. Organisms and bacteria are everywhere and are always surrounding you, so I think no matter how hard you scrub there will never be a count or organisms. ‘zero’ 5. The importance of hand scrubbing was first introduced as a clinical procedure in 1846 by Ignaz Semmelweis when he showed that puerperal fever (childbed fever) cases decreased when medical students cleaned their hands with lime and chloride before delivering babies. However, hand washing and disinfection to prevent spread of disease and illness was not practiced until the later part of the 19th century due to the efforts of Pasteur and Lister. Today it is a routine practice
helps in the removal of both transient and resident microorganisms that can cause disease or infection Part 2 Questions 1. Some microorganisms that are commonly found on the skin of the hands are: Gram-positive cocci: Staphylococcus, Gram-negative bacilli: Klebsiella-Enterobacter sp., Acinetobacter sp., Pseudomonas sp., Proteus-providencia sp. and Yeast. Staphylococcus aureus is the only true pathogenic organism included in the resident microflora group of skin, and some, but not all, individuals carry Staphylococcus aureus on their skin. The population of Staphylococcus epidermidis far out numbers S. aureus on healthy skin 2. The cultural medium used in the hand scrub experiment was veal infusion agar (VIA) pours in 50̊ C water bath. The plates sat until solid and were incubated to grow the organisms isolated from the hands for 48 hours at 37̊ C. 3. Escherichia coli, S. minus and yeasts can also grow in this medium. 4. I would expect that there will never be ‘zero’ organisms if you continued scrubbing your hands in this experiment. Organisms and bacteria are everywhere and are always surrounding you, so I think no matter how hard you scrub there will never be a ‘zero’ count or organisms. 5. The importance of hand scrubbing was first introduced as a clinical procedure in 1846 by Ignaz Semmelweis when he showed that puerperal fever (childbed fever) cases decreased when medical students cleaned their hands with lime and chloride before delivering babies. However, hand washing and disinfection to prevent spread of disease and illness was not practiced until the later part of the 19th century due to the efforts of Pasteur and Lister. Today it is a routine practice
BIO%20162%20Lab%20Assignment.docx