Risk assessment COSHH risk assessment - University of Oxford COSHH Assessment Form Description of procedure Substances used Determination of bacterial antimicrobial 1. Cultured bacteria susceptibilities by disk diffusion 2. Antimicrobial impregnated disks Quantities of chemicals used Frequency of SOP use 5ml of bacterial suspensions Hazards identified Could a less hazardous substance be 1. Autoclaved liquid used instead? 2. Potentially infectious material in sample No Potentially pathogenic bacteria What measures have you to control risk? 1. Training in good laboratory practices (GLP) 2. Appropriate PPE (lab coat, gloves, eye protection) 3. Use of biosafety cabinet for reading of plates / follow-up of BSL-3 organisms (e.g. B. pseudomallei) Checks on control measures Observation and supervision by senior staff Is health surveillance required? Training requirements No 1. GLP 2. Specific training in this SOP Emergency procedures Waste disposal procedures 1. Report all incidents to Safety Adviser 1. Sharps discarded into appropriate rigid 2. Use eyewash for splashes containers for incineration 3. Clean up spills using 1% Virkon or 2. Infectious waste discarded into autoclave bags chemical spill kit or 1% Virkon solution prior to autoclaving and subsequent incineration 3. Chemical waste disposed of according to manufacturer’s instructions
Risk assessment COSHH risk assessment - University of Oxford COSHH Assessment Form Description of procedure Determination of bacterial antimicrobial susceptibilities by disk diffusion Substances used 1. Cultured bacteria 2. Antimicrobial impregnated disks Quantities of chemicals used 5ml of bacterial suspensions Frequency of SOP use Hazards identified 1. Autoclaved liquid 2. Potentially infectious material in sample Potentially pathogenic bacteria Could a less hazardous substance be used instead? No What measures have you to control risk? 1. Training in good laboratory practices (GLP) 2. Appropriate PPE (lab coat, gloves, eye protection) 3. Use of biosafety cabinet for reading of plates / follow-up of BSL-3 organisms (e.g. B. pseudomallei) Checks on control measures Observation and supervision by senior staff Is health surveillance required? No Training requirements 1. GLP 2. Specific training in this SOP Emergency procedures 1. Report all incidents to Safety Adviser 2. Use eyewash for splashes 3. Clean up spills using 1% Virkon or chemical spill kit Waste disposal procedures 1. Sharps discarded into appropriate rigid containers for incineration 2. Infectious waste discarded into autoclave bags or 1% Virkon solution prior to autoclaving and subsequent incineration 3. Chemical waste disposed of according to manufacturer’s instructions
Antimicrobial_Sensitivity_Disk_Difussion.docx