and outcome assessment Xu M Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low high risk of low risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of Insufficient culture, of data are recorded in cell cell blinding to information main text or suspension suspension solvent, study group provided and in supplementary - ZIKV, - ZIKV, involving about titration blinded material control control plate organoids, number of described fashion author organoids (6 (MOI = 0.04 indicated organoids to 0.08) that used) investigators were not blinded to allocation during experiments and outcome assessment Yoon KJ Definitely Definitely Definitely Definitely Definitely Definitely Definitely low risk low risk of low risk of low high risk of low risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - Number of - - Analysis outcomes outlined Homogenous Homogenous - Same evidence of organoids of data are recorded in cell cell blinding to provided for of GFP and main text or suspension suspension solvent, study group all ZIKV-NS2A by Zen supplementary - - involving experiments or or ImageJ material of of plate organoids, in software GPF and GPF and author ventricular ZIKV-NS2A or ZIKV-NS2A or indicated radial glia DENV-NS2A DENV-NS2A that in via via investigators forebrain were not organoids by control control blinded to allocation during experiments and outcome assessment
and outcome assessment Xu M Definitely low risk of bias - Homogenous cell suspension - ZIKV, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, control low risk of bias - Same solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Probably low risk of bias - Insufficient information provided about number of organoids (6 organoids used) Definitely low risk of bias - Viral culture, and titration described (MOI = 0.04 to 0.08) Definitely low risk of bias - Analysis of data in blinded fashion Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material Yoon KJ Definitely low risk of bias - Homogenous cell suspension - of GPF and ZIKV-NS2A or DENV-NS2A via control Definitely low risk of bias - Homogenous cell suspension - of GPF and ZIKV-NS2A or DENV-NS2A via control low risk of bias - Same solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Definitely low risk of bias - Number of organoids provided for all experiments Definitely low risk of bias - of GFP and ZIKV-NS2A or in ventricular radial glia in forebrain organoids by Definitely low risk of bias - Analysis of data by Zen or ImageJ software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material
Bayu.Sutarjono-RLRA.docx