Li C Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low high risk of high risk of low risk of low risk of of bias bias bias risk of bias bias (not bias bias - All measured - - bias - No recorded) - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of - culture, of data are recorded in cell cell media, blinding to Insufficient main text or suspension suspension solvent, study group information and by supplementary - ZIKV, - ZIKV, involving provided titration Imaris or material control control plate organoids, about described ImageJ author number of (MOI = 1) software indicated organoids that (number of investigators organoids were not not blinded to recorded) allocation during experiments and outcome assessment Li Y Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low high risk of high risk of low risk of low risk of of bias bias bias risk of bias bias (not bias bias - All measured - - bias - No recorded) - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of - culture, of data are recorded in cell cell media, blinding to Insufficient main text or suspension suspension solvent, study group information and by supplementary - ZIKV, - ZIKV, involving provided titration ImageJ material control control plate organoids, about described software author number of (MOI = 1) indicated organoids that (number of investigators organoids were not not blinded to recorded) allocation **Apoptosis during experiments by and outcome CASP3 not assessment quantified **Full statistical method not disclosed for each experiment Qian X Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low low risk of high risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - - - Viral - Analysis outcomes outlined Homogenous Homogenous - Same Investigators Insufficient culture, of data are recorded in cell cell media, blinded to information main text or suspension suspension solvent, culture provided and by supplementary - ZIKV, - ZIKV, conditions about titration ImageJ material control control plate number of described software organoids (1x or 0.25x (3, 5, or 6 dilution) organoids used) **ZIKV-C strain limited to SOX2 and TBR2 staining **Statistical
Li C Definitely low risk of bias - Homogenous cell suspension - ZIKV, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, control low risk of bias - Same media, solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Probably high risk of bias (not recorded) - Insufficient information provided about number of organoids (number of organoids not recorded) Definitely low risk of bias - Viral culture, and titration described (MOI = 1) Definitely low risk of bias - Analysis of data by Imaris or ImageJ software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material Li Y Definitely low risk of bias - Homogenous cell suspension - ZIKV, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, control low risk of bias - Same media, solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Probably high risk of bias (not recorded) - Insufficient information provided about number of organoids (number of organoids not recorded) **Apoptosis by CASP3 not quantified **Full statistical method not disclosed for each experiment Definitely low risk of bias - Viral culture, and titration described (MOI = 1) Definitely low risk of bias - Analysis of data by ImageJ software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material Qian X Definitely low risk of bias - Homogenous cell suspension - ZIKV, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, control low risk of bias - Same media, solvent, plate Definitely low risk of bias - Investigators blinded to culture conditions Probably high risk of bias - Insufficient information provided about number of organoids (3, 5, or 6 organoids used) **ZIKV-C strain limited to SOX2 and TBR2 staining **Statistical Definitely low risk of bias - Viral culture, and titration described (1x or 0.25x dilution) Definitely low risk of bias - Analysis of data by ImageJ software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material
Bayu.Sutarjono-RLRA.docx