Centrifugation at full speed for 1 min was done. The QIAamp mini spin column was placed in a clean 1.5 ml micro centrifuge tube. The QIAamp mini spin column was carefully opened and 100 buffer AE were added. μl The QIAamp mini spin column was Incubated at room temperature for 1 min, and then centrifuged at 8000 rpm for 1 min. 3-4. Preparation of duplex PCR Master: Mix for each of and (cdtB and cdtC) and (C. coli glyA C. jejuni hipO), (virB11 and genes according to flaA) GoTaq® Hot Start Green Master Mix. (Iroala 2012). 3-5. Agarose gel electrophoreses et al., Electrophoresis grade agarose (1.5 g) was prepared in 100 ml TBE buffer in a sterile flask, 0.5μg/ml Ethidium bromide was added and mixed thoroughly. Twenty of each PCR product samples, negative control μl and positive control were loaded to the gel. The gel was photographed by a gel documentation system and the data was analyzed. Results: Out of 230 rabbit samples screened for the presence of Campylobacters, 58 samples yielded characteristic colonies on CCDA Campylobacter plates after 48 hours of incubation (Table, 4). They had characteristic corkscrew motility observed by the phase contrast microscope. The biochemical tests for isolates were found positive for catalase, oxidase, and nitrate. None of the isolates revealed positive reaction for urease activity (Table, 5). Out of the 51 (22.17%) isolates, 27 (52.94%) were Campylobacter identified as , 17 (33.33%) isolates as (126 bp), C. jejuni (323bp) C.coli
Centrifugation at full speed for 1 min was done. The QIAamp mini spin column was placed in a clean 1.5 ml micro centrifuge tube. The QIAamp mini spin column was carefully opened and 100 μl buffer AE were added. The QIAamp mini spin column was Incubated at room temperature for 1 min, and then centrifuged at 8000 rpm for 1 min. 3-4. Preparation of duplex PCR Master: Mix for each of (C. coli glyA and C. jejuni hipO), (cdtB and cdtC) and (virB11 and flaA) genes according to GoTaq® Hot Start Green Master Mix. 3-5. Agarose gel electrophoreses (Iroala et al., 2012). Electrophoresis grade agarose (1.5 g) was prepared in 100 ml TBE buffer in a sterile flask, 0.5μg/ml Ethidium bromide was added and mixed thoroughly. Twenty μl of each PCR product samples, negative control and positive control were loaded to the gel. The gel was photographed by a gel documentation system and the data was analyzed. Results: Out of 230 rabbit samples screened for the presence of Campylobacters, 58 samples yielded characteristic Campylobacter colonies on CCDA plates after 48 hours of incubation (Table, 4). They had characteristic corkscrew motility observed by the phase contrast microscope. The biochemical tests for isolates were found positive for catalase, oxidase, and nitrate. None of the isolates revealed positive reaction for urease activity (Table, 5). Out of the 51 (22.17%) Campylobacter isolates, 27 (52.94%) were identified as C. jejuni (323bp) , 17 (33.33%) isolates as C.coli (126 bp),
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