Full description of changes to non-surgical procedures as listed in the BELOW. If substances are being administered to animals please include details of route, volumes, frequency, intervals and duration. Summary description of changes to non-surgical procedures. Type of non-surgical Expected impacts of the Expected frequency of Refinement to procedure to be carried out procedure adverse impacts minimise impacts Good handling to minimise Some discomfort on each Minor discomfort discomfort and observation after occasion. Substance in airway or e.g. gavage rarely substance enters airway dosing with humane killing of oesophageal damage in less than or oesophagus is damaged any animal showing signs of 1 in 1000 administrations. or damage. Who will perform new procedures? Provide details of their skills and specific training and competency in these procedures SURGICAL PROCEDURES GIVE DETAILS OF CHANGE – Full description of changes to all surgical procedures listed BELOW Type of surgical Expected impacts of the Expected frequency of Refinement taken to procedure to be carried procedure adverse impacts minimise impacts out e.g. insertion of catheter Pain Always Analgesia Who will perform these new procedures? Provide details of their skills, specific training and competency in these procedures ANAESTHETICS AND NEUROMUSCULAR BLOCKADE Species Agent (s) Dose Route Frequency Duration Ketamine 80 mg/kg ip Single injection is sufficient for the 10 minute e.g. Rat Once only procedure Xylazine 10 mg/kg ip Who will perform these new procedures? Provide details of their specific training and competency in these procedures ANALGESICS DETAIL CHANGES TO Analgesics to be used intra - and post-operatively – Species Dose Route Frequency & Duration Initial dose given at induction of anaesthesia then e.g. Mouse buprenorphine 0.05-0.1mg/kg Sub cut continued every 8 for 3 days post-op Who will perform these new procedures? Provide details of their specific training and competency in these procedures 9. ANIMAL WELL-BEING A. Attach appropriate Cover Sheet and Recording Sheet for project monitoring. Attached [ ] Animal Monitoring Templates can be downloaded from
Full description of changes to non-surgical procedures as listed in the BELOW. If substances are being administered to animals please include details of route, volumes, frequency, intervals and duration. Summary description of changes to non-surgical procedures. Type of non-surgical procedure to be carried out Expected impacts of the procedure Expected frequency of adverse impacts Refinement to minimise impacts e.g. gavage Minor discomfort rarely substance enters airway or oesophagus is damaged Some discomfort on each occasion. Substance in airway or oesophageal damage in less than 1 in 1000 administrations. Good handling to minimise discomfort and observation after dosing with humane killing of any animal showing signs of or damage. Who will perform new procedures? Provide details of their skills and specific training and competency in these procedures SURGICAL PROCEDURES – GIVE DETAILS OF CHANGE Full description of changes to all surgical procedures listed BELOW Type of surgical procedure to be carried out Expected impacts of the procedure Expected frequency of adverse impacts Refinement taken to minimise impacts e.g. insertion of catheter Pain Always Analgesia Who will perform these new procedures? Provide details of their skills, specific training and competency in these procedures ANAESTHETICS AND NEUROMUSCULAR BLOCKADE Species Agent (s) Dose Route Frequency Duration e.g. Rat Ketamine Xylazine 80 mg/kg 10 mg/kg ip ip Once only Single injection is sufficient for the 10 minute procedure Who will perform these new procedures? Provide details of their specific training and competency in these procedures ANALGESICS – DETAIL CHANGES TO Analgesics to be used intra - and post-operatively Species Dose Route Frequency & Duration e.g. Mouse buprenorphine 0.05-0.1mg/kg Sub cut Initial dose given at induction of anaesthesia then continued every 8 for 3 days post-op Who will perform these new procedures? Provide details of their specific training and competency in these procedures 9. ANIMAL WELL-BEING A. Attach appropriate Cover Sheet and Recording Sheet for project monitoring. Attached [ ] Animal Monitoring Templates can be downloaded from
Amendment-form-MAJOR.docx