Heat with agitation to dissolve. Adjust pH to Tryptose 15 g 7.6±0.2. Autoclave 15 minutes at 121°C. Yeast extract 5 g Cool in a water bath to 50°C. Soytone 5 g Add 80 ml of D-cycloserine solution (1 g in 200 Ferric ammonium citrate 1 g ml distilled Sodium metabisulfite 1 g Add 80 ml of 50% egg yolk emulsion. Agar 20g Pour plates. Distilled water 900ml Positive: Black colonies surrounded by an opaque white zone are indicative of lecithinase activity.
Tryptose Yeast extract Soytone Ferric ammonium citrate Sodium metabisulfite Agar Distilled water 15 g 5 g 5 g 1 g 1 g 20g 900ml Heat with agitation to dissolve. Adjust pH to 7.6±0.2. Autoclave 15 minutes at 121°C. Cool in a water bath to 50°C. Add 80 ml of D-cycloserine solution (1 g in 200 ml distilled Add 80 ml of 50% egg yolk emulsion. Pour plates. Positive: Black colonies surrounded by an opaque white zone are indicative of lecithinase activity.
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