LMW1 ● LMW2 ● Upon Reduction HC LC 1/2Fc Fd (50732 Da) (23195 Da) (26,767 Da) (23,983 Da) Main ● ● LMW1 ● ● ● LMW2 ● ● electropherogram of neat IgG1 A (Top) and IgG1 B (Bottom) SEC Main, LMW1, and Figure S5: nrCE-SDS LMW2 fractions Figure S6: rCE-SDS electropherogram of isolated SEC Main, LMW1, and LMW2 fractions for IgG1 A (Top) and IgG1 B (Bottom) Figure S7: nrCE-SDS (Top) and rCE-SDS (bottom) of HMW species showed higher level of fragments compared to the Main species. . HMW fractions of Day 14 stressed samples showed 10 15 % total – fragments. Figure S8: The corrected fragment levels (%Fragment - %Fragment from Day 0) by CE-SDS plotted against change in fragment (%) by SEC. Both nrCE-SDS and rCE-SDS linearly correlate with SEC with R2 > 0.99, respectively.
LMW1 ● LMW2 ● Upon Reduction HC (50732 Da) LC (23195 Da) 1/2Fc (26,767 Da) Fd (23,983 Da) Main ● ● LMW1 ● ● ● LMW2 ● ● Figure S5: nrCE-SDS electropherogram of neat IgG1 A (Top) and IgG1 B (Bottom) SEC Main, LMW1, and LMW2 fractions Figure S6: rCE-SDS electropherogram of isolated SEC Main, LMW1, and LMW2 fractions for IgG1 A (Top) and IgG1 B (Bottom) Figure S7: nrCE-SDS (Top) and rCE-SDS (bottom) of HMW species showed higher level of fragments compared to the Main species. . HMW fractions of Day 14 stressed samples showed 10 – 15 % total fragments. Figure S8: The corrected fragment levels (%Fragment - %Fragment from Day 0) by CE-SDS plotted against change in fragment (%) by SEC. Both nrCE-SDS and rCE-SDS linearly correlate with SEC with R2 > 0.99, respectively.
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