Table A.2 No changes of Ppargc1a and target genes in tissue of Fus∆NLS mice Fus∆NLS Region Gene Fus+/+ Gene Fus+/+ Canonical 100 ± 76.96 ± CNS-specific 100 ± 108.35 ± Spinal cord Ppargc1a 9.45 6.11 Ppargc1a 4.23 12.11 Canonical 100 ± 90.27 ± CNS-specific 100 ± 100.45 ± Cortex Ppargc1a 5.24 6.24 Ppargc1a 8.22 6.24 Canonical 100 ± 74.77 ± 100 ± 105.18 ± Gastrocnemius Tfam Ppargc1a 14.36 7.52 12.58 19.7 Canonical 100 ± 172.66 ± 100 ± 135.29 ± BAT Ucp1 Ppargc1a 11.97 43.59 6.36 16.71 Spinal cord, cortex, gastrocnemius and brown adipose tissue (BAT) of mice were lysed and mRNA of canonical Ppargc1a, CNS-specific Ppargc1a, Tfam and Ucp1 were quantified by qPCR and normalized to Tbp and Polr2a. Data are presented as mean in % of control ± SEM, n = 5-6.
Table A.2 No changes of Ppargc1a and target genes in tissue of Fus∆NLS mice Region Gene Fus+/+ Gene Fus+/+ Spinal cord Canonical Ppargc1a 100 ± 9.45 76.96 ± 6.11 CNS-specific Ppargc1a 100 ± 4.23 108.35 ± 12.11 Cortex Canonical Ppargc1a 100 ± 5.24 90.27 ± 6.24 CNS-specific Ppargc1a 100 ± 8.22 100.45 ± 6.24 Gastrocnemius Canonical Ppargc1a 100 ± 14.36 74.77 ± 7.52 Tfam 100 ± 12.58 105.18 ± 19.7 BAT Canonical Ppargc1a 100 ± 11.97 172.66 ± 43.59 Ucp1 100 ± 6.36 135.29 ± 16.71 Spinal cord, cortex, gastrocnemius and brown adipose tissue (BAT) of Fus∆NLS mice were lysed and mRNA of canonical Ppargc1a, CNS-specific Ppargc1a, Tfam and Ucp1 were quantified by qPCR and normalized to Tbp and Polr2a. Data are presented as mean in % of control ± SEM, n = 5-6.
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