high incidence in liver and intestinal samples 50% while in cloacal swabs was 35.71%. A putative virulence gene associated with adhesion of the pathogen to intestinal epithelial cells (Rosenquist This gene 2003). et al., is 100% conserved among and isolates of diverse C. jejuni C. coli sources; therefore, it was used to detect virulent isolates of both species (Datta Cytolethal distending toxin (CDT) is widely 2003). et al., distributed among Gram-negative bacteria (Guo 2010) and is the et al., best characterized of the toxins produced by spp. It has Campylobacter been described as an important virulence factor of this pathogen (Asakura 2008). holotoxin, composed of three subunits et al., CDT encoded by the genes, causes eukaryotic cells to cdtA, cdtB and cdtC arrest in the G2/M phase of the cell cycle, preventing them from entering mitosis and consequently leading to cell death (Zilbauer 2008). et al., cytolethal distending toxins showed at 555 bp for and at C.jejuni CdtC 495 bp for of the genus 555 bp and 495 bp CdtB Campylobacter amplicons were confirmed. strains were about the same size as the C. lari C. jejuni and C. coli products during the PCR step did not amplify this product. Thus, the assay could be used to discriminate between and the C. lari C. jejuni-C. isolates. The remaining seven strains were belonging coli Campylobacter to other species in PCR assays. However, reducing the Campylobacter primer concentration from 0.25 to 0.2 during the first PCR step was μm sometimes necessary to reduce the number of nonspecific amplicons (Woz´niak and Wieliczko, 2011). We concluded that are highly prevalent in rabbit C. jejuni and C. coli farms in Egypt. Control measures for exposure and contamination of the rabbit supply should be identified so that methods can be developed to
high incidence in liver and intestinal samples 50% while in cloacal swabs was 35.71%. A putative virulence gene associated with adhesion of the pathogen to intestinal epithelial cells (Rosenquist et al., 2003). This gene is 100% conserved among C. jejuni and C. coli isolates of diverse sources; therefore, it was used to detect virulent isolates of both species (Datta et al., 2003). Cytolethal distending toxin (CDT) is widely distributed among Gram-negative bacteria (Guo et al., 2010) and is the best characterized of the toxins produced by Campylobacter spp. It has been described as an important virulence factor of this pathogen (Asakura et al., 2008). CDT holotoxin, composed of three subunits encoded by the cdtA, cdtB and cdtC genes, causes eukaryotic cells to arrest in the G2/M phase of the cell cycle, preventing them from entering mitosis and consequently leading to cell death (Zilbauer et al., 2008). C.jejuni cytolethal distending toxins showed at 555 bp for CdtC and at 495 bp for CdtB of the genus Campylobacter 555 bp and 495 bp amplicons were confirmed. C. lari strains were about the same size as the C. jejuni and C. coli products during the PCR step did not amplify this product. Thus, the assay could be used to discriminate between C. lari and the C. jejuni-C. coli isolates. The remaining seven Campylobacter strains were belonging to other Campylobacter species in PCR assays. However, reducing the primer concentration from 0.25 to 0.2 μm during the first PCR step was sometimes necessary to reduce the number of nonspecific amplicons (Woz´niak and Wieliczko, 2011). We concluded that C. jejuni and C. coli are highly prevalent in rabbit farms in Egypt. Control measures for exposure and contamination of the rabbit supply should be identified so that methods can be developed to
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