APPENDIX I EBMT-CTIWP Retrospective Study: Consensus guidelines of the European Group for Blood and Marrow Transplantation for monitoring post-transplant immune reconstitution Team details: CIC n° Hospital Unit Name Email Telephone Fax Principal investigator Data manager Current practice in your centre for the monitoring of post-transplant immune reconstitution: ASSAYS Clinical Are you Comments HSCT HSCT HSCT HSCT vs investigational use from from from from planning to not available sibling volunteer vs implement donor: unrelated unit: this test (in Y N donor: donor: Y N vs vs house or with Y N Y N vs vs other centers) in the next year?Are you planning to implement this test (in house or in coll. With other centers) in the next year? Hematology: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ CBC with Investigational No No No No No □ □ □ □ □ □ differential Not available □ (neutrophils, lymphocytes) Clinical laboratory: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ Investigational No No No No No Immunoglobulins □ □ □ □ □ □ Not available □ Clinical Yes Yes Yes Yes Yes Flow-cytometry: □ □ □ □ □ □ Lymphocyte subsets Investigational No No No No No □ □ □ □ □ □ (T, B and NK Not available □ absolute counts) Quantitative tests Flow-cytometry: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ T-cell subsets Investigational No No No No No □ □ □ □ □ □ Not available □ Flow-cytometry: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ B-cell subsets Investigational No No No No No □ □ □ □ □ □ Not available □ Flow-cytometry: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ Investigational No No No No No NK-cell subsets □ □ □ □ □ □ Not available □ Molecular tests: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ TRECs and KRECs Investigational No No No No No □ □ □ □ □ □ Not available □ Engraftment Molecular tests: Clinical Yes Yes Yes Yes Yes □ □ □ □ □ □ monitoring STR, qPCR, Fish, Investigational No No No No No □ □ □ □ □ □
APPENDIX I EBMT-CTIWP Retrospective Study: Consensus guidelines of the European Group for Blood and Marrow Transplantation for monitoring post-transplant immune reconstitution Team details: CIC n° Hospital Unit Name Email Telephone Fax Principal investigator Data manager Current practice in your centre for the monitoring of post-transplant immune reconstitution: ASSAYS Clinical vs investigational use vs not available Are you planning to implement this test (in house or with other centers) in the next year?Are you planning to implement this test (in house or in coll. With other centers) in the next year? Comments HSCT from sibling donor: Y vs N HSCT from volunteer unrelated donor: Y vs N HSCT from donor: Y vs N HSCT from unit: Y vs N Quantitative tests Hematology: CBC with differential (neutrophils, lymphocytes) □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Clinical laboratory: Immunoglobulins □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Flow-cytometry: Lymphocyte subsets (T, B and NK absolute counts) □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Flow-cytometry: T-cell subsets □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Flow-cytometry: B-cell subsets □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Flow-cytometry: NK-cell subsets □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Molecular tests: TRECs and KRECs □ Clinical □ Investigational □ Not available □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No Engraftment monitoring Molecular tests: STR, qPCR, Fish, □ Clinical □ Investigational □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No □ Yes □ No
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