Enzyme digest Fragment sizes BamHI EcoRI + BamHI EcoRI What fragment sizes could be generated from a partial digest? Only indicate the BamHI sizes of intermediate fragments which would not be obtained following a complete digest. Draw all possible maps, which could correspond to the results indicated in the above table for the digest alone. (DO NOT include mirror images) BamHI It was determined that the enzyme cuts at 0.5Kbp on the map shown above. Indicate XhoI which fragment would be cut by and what sizes would be generated in each of BamHI XhoI the maps provided for question 3. (Ex. 4Kbp BamHI 3 + 1 Kbp). → A complete digest with + of 12µg of the above fragment was performed. EcoRI BamHI Indicate the amount in µg of each of the fragments which would be obtained. The circular 10Kbp DNA molecule shown below has cleavage sites for and BamHI EcoRI. Complete the table to indicate the fragment sizes which would be observed on an agarose gel following each of the indicated digests. Note, if different fragments of the same size are generated, the size should only be indicated once. (For example do not indicate 2 and 2Kbp)
Enzyme digest Fragment sizes BamHI EcoRI BamHI + EcoRI What fragment sizes could be generated from a BamHI partial digest? Only indicate the sizes of intermediate fragments which would not be obtained following a complete digest. Draw all possible maps, which could correspond to the results indicated in the above table for the BamHI digest alone. (DO NOT include mirror images) It was determined that the enzyme XhoI cuts at 0.5Kbp on the map shown above. Indicate which BamHI fragment would be cut by XhoI and what sizes would be generated in each of the maps provided for question 3. (Ex. 4Kbp BamHI → 3 + 1 Kbp). A complete digest with EcoRI + BamHI of 12µg of the above fragment was performed. Indicate the amount in µg of each of the fragments which would be obtained. The circular 10Kbp DNA molecule shown below has cleavage sites for BamHI and EcoRI. Complete the table to indicate the fragment sizes which would be observed on an agarose gel following each of the indicated digests. Note, if different fragments of the same size are generated, the size should only be indicated once. (For example do not indicate 2 and 2Kbp)
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