Figure S19. Confocal fluorescence images of HeLa cells stained with (a) LysoTracker Green DND-26 (100 nM, = 488 nm, = 500–530 nm) and (b) λex λem (1 = 543 nm, = 560–610 nm) at 37 °C for 30 min. (c) Merged Lyso-Red μM, λex λem image of (a) and (b). (d) Bright field image. (e) Correlation plot of LysoTracker Green DND-26 and intensities. (f) Intensity profile of regions of interest (ROI) Lyso-Red across Hela cells. Scale bar: 25 μm. Figure S20. Confocal fluorescence images of HeLa cells stained with (a) LysoTracker Green DND-26 (100 nM, = 488 nm, = 500–530 nm) and (b) λex λem (10 = 638 nm, = 652–732 nm) at 37 °C for 30 min. (c) Lyso-NIR μM, λex λem Merged image of (a) and (b). (d) Bright field image. (e) Correlation plot of LysoTracker Green DND-26 and intensities. (f) Intensity profile of regions Lyso-NIR of interest (ROI) across Hela cells. Scale bar: 25 μm.
Figure S19. Confocal fluorescence images of HeLa cells stained with (a) LysoTracker Green DND-26 (100 nM, λex = 488 nm, λem = 500–530 nm) and (b) Lyso-Red (1 μM, λex = 543 nm, λem = 560–610 nm) at 37 °C for 30 min. (c) Merged image of (a) and (b). (d) Bright field image. (e) Correlation plot of LysoTracker Green DND-26 and Lyso-Red intensities. (f) Intensity profile of regions of interest (ROI) across Hela cells. Scale bar: 25 μm. Figure S20. Confocal fluorescence images of HeLa cells stained with (a) LysoTracker Green DND-26 (100 nM, λex = 488 nm, λem = 500–530 nm) and (b) Lyso-NIR (10 μM, λex = 638 nm, λem = 652–732 nm) at 37 °C for 30 min. (c) Merged image of (a) and (b). (d) Bright field image. (e) Correlation plot of LysoTracker Green DND-26 and Lyso-NIR intensities. (f) Intensity profile of regions of interest (ROI) across Hela cells. Scale bar: 25 μm.
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