Supplementary Table 1. Antibodies and their source, pre-treatment and visualization methods used in immunohistochemistrya Antigen Antibody source, Epitope Detection system Platform clone, and retrieval dilution methodb CD3 Dako rabbit pH 8 Ventana Benchmark – polyclonal 1:400 mouse alkaline XT phosphatase-labeled polymer (Ventana) MUM-1 Dako pH 8 Ventana Benchmark – monoclonal, mouse alkaline XT MUM1p, 1:50 phosphatase-labeled polymer, with proprietary amplifier (Ventana) CD18 Peter Moore, UC pH 6 Goat/anti-mouse Dako Autostainer (canine) Davis, horseradish monoclonal peroxidase-labeled CA1.4E9, 1:80 polymer (Dako) CD79a Dako - mouse pH 9 Goat/anti-mouse Dako Autostainer monoclonal horseradish HM57, 1:200 peroxidase-labeled polymer (Dako) LLC Dako rabbit pH 6 Ventana Benchmark – polyclonal,1:12000 alkaline XT phosphatase-labeled polymer (Ventana) a Processed 4 sections from paraffin-embedded tissues were mounted on μm silane-treated glass slides, deparaffinized and rehydrated prior to immunochemistry. b Heat-induced.
Supplementary Table 1. Antibodies and their source, pre-treatment and visualization methods used in immunohistochemistrya Antigen Antibody source, clone, and dilution Epitope retrieval methodb Detection system Platform CD3 Dako – rabbit polyclonal 1:400 pH 8 alkaline phosphatase-labeled polymer (Ventana) Ventana Benchmark XT MUM-1 Dako – mouse monoclonal, MUM1p, 1:50 pH 8 alkaline phosphatase-labeled polymer, with proprietary amplifier (Ventana) Ventana Benchmark XT CD18 (canine) Peter Moore, UC Davis, mouse monoclonal CA1.4E9, 1:80 pH 6 Goat/anti-mouse horseradish peroxidase-labeled polymer (Dako) Dako Autostainer CD79a Dako - mouse monoclonal HM57, 1:200 pH 9 Goat/anti-mouse horseradish peroxidase-labeled polymer (Dako) Dako Autostainer LLC Dako – rabbit polyclonal,1:12000 pH 6 alkaline phosphatase-labeled polymer (Ventana) Ventana Benchmark XT a Processed 4 μm sections from paraffin-embedded tissues were mounted on silane-treated glass slides, deparaffinized and rehydrated prior to immunochemistry. b Heat-induced.
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