Table (2): Oligonucleotide primers sequences. Reference Length of Primer sequence Target gene amplified product (5'-3') Wang et al., 323 bp ACTTCTTTATTGCTTGCTGC C. jejuni hipO 2002 GCCACAACAAGTAAAGAAGC 126 bp GTAAAACCAAAGCTTATCGTG C. coli glyA TCCAGCAATGTGTGCAATG Datta et al., 855 bp FlaA 2003 494 bp virB11 Bang et al., 495 bp GTTAAAATCCCCTGCTATCAACCA CdtB 2003 555 bp cdtC TTGCACATAACCAAAAGGAAG Table (3): Cycling conditions of the different primers during PCR Gene Primary Secondary Annealing Extension No. of Final Reference denaturation denaturation cycles extension HipO 94˚C 95˚C 55˚C 72˚C 35 72˚C Wang et and 6 min. 30 sec. 30 sec. 30 sec. 10 min. al., 2002 glyA
Table (2): Oligonucleotide primers sequences. Reference Length of amplified product Primer sequence (5'-3') Target gene Wang et al., 2002 323 bp ACTTCTTTATTGCTTGCTGC C. jejuni hipO GCCACAACAAGTAAAGAAGC 126 bp GTAAAACCAAAGCTTATCGTG C. coli glyA TCCAGCAATGTGTGCAATG Datta et al., 2003 855 bp FlaA 494 bp virB11 Bang et al., 2003 495 bp GTTAAAATCCCCTGCTATCAACCA CdtB 555 bp cdtC TTGCACATAACCAAAAGGAAG Table (3): Cycling conditions of the different primers during PCR Gene Primary denaturation Secondary denaturation Annealing Extension No. of cycles Final extension Reference HipO and glyA 94˚C 6 min. 95˚C 30 sec. 55˚C 30 sec. 72˚C 30 sec. 35 72˚C 10 min. Wang et al., 2002
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