Supplementary material: State of the art in currently available amylase detection technique Assay Design Detection Limits of detection Specificity Ref. Sequential Process Technique Flow system using a AMP LOD: 2nkat/mL Any 141 α-glucosidase peroxide electrode and converts maltose to (0.117.64 units/mL) enzyme membrane with when reaction time α-D-glucose. glucose oxidase, Mutarotase 5 min and converts 0.5 nkat/mL optionally mutarotase to units/mL) when cross-linked by which is reaction time 30 determined via min glucose oxidase LR: 0.1-3 mmol/L Screen-printed AMP LOD: 5 units/mL Any 142 α-glucosidase electrodes with converts maltose to immobilized LR: 5-250 units/mL α-D-glucose. glucose Mutarotase oxidase and mutarotase converts modified with Prussian to Blue which is determined via glucose oxidase Flow-injection device AMP LOD: Any 143 α-glucosidase maltopentaose as converts maltose to substrate. 5-d-glucose. LR: 0-30 units/mL α-glucosidase immobilised on Glucose oxidase membrane. coverts 5-d-glucose Glucose oxidase to gluconic acid and immobilized on hydrogen peroxide electrode which is measured Spectrophotometric flow Amylose incubated SIA/FIA LOD: 0.0048 FAU Any 144 injection measuring brick with sample to LR: 0.005-0.06 FAU red complex formation produce maltose. at 540nm 3,5 and maltose boiled Use of portable personal Sample, LOD: 20 U/L Any 145 glucose meter and maltopentaose LR: 2.2-27.8 mM incubated 15 min at 370C. Flat-chip micro analytical Maltose LR: 0-190 kU/L Any 143 sensor used as part of a phosphorylase and phosphorylates Lateral Systems. maltose. Glucose flow Pre-column and oxidase converts electrode phosphorylated incorporated into a flow maltose to cell where maltose gluconic acid and
Supplementary material: State of the art in currently available amylase detection technique Assay Design Detection Limits of detection Specificity Ref. Sequential Process Technique Flow system using a peroxide electrode and enzyme membrane with glucose oxidase, and optionally mutarotase cross-linked by α-glucosidase converts maltose to α-D-glucose. Mutarotase converts to which is determined via glucose oxidase AMP LOD: 2nkat/mL (0.117.64 units/mL) when reaction time 5 min 0.5 nkat/mL units/mL) when reaction time 30 min LR: 0.1-3 mmol/L Any 141 Screen-printed electrodes with immobilized glucose oxidase and mutarotase modified with Prussian Blue α-glucosidase converts maltose to α-D-glucose. Mutarotase converts to which is determined via glucose oxidase AMP LOD: 5 units/mL LR: 5-250 units/mL Any 142 Flow-injection device maltopentaose as substrate. α-glucosidase immobilised on membrane. Glucose oxidase immobilized on electrode α-glucosidase converts maltose to 5-d-glucose. Glucose oxidase coverts 5-d-glucose to gluconic acid and hydrogen peroxide which is measured AMP LOD: LR: 0-30 units/mL Any 143 Spectrophotometric flow injection measuring brick red complex formation at 540nm Amylose incubated with sample to produce maltose. 3,5 and maltose boiled SIA/FIA LOD: 0.0048 FAU LR: 0.005-0.06 FAU Any 144 Use of portable personal glucose meter Sample, and maltopentaose incubated 15 min at 370C. LOD: 20 U/L LR: 2.2-27.8 mM Any 145 Flat-chip micro analytical sensor used as part of a Systems. Pre-column and electrode incorporated into a flow cell where maltose Maltose phosphorylase phosphorylates maltose. Glucose oxidase converts phosphorylated maltose to gluconic acid and and Lateral flow LR: 0-190 kU/L Any 143
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