unsheathed flagella, with no periplasmic fibres, and appeared coccoid after 10-12 days of incubation. Phylogenetic analyses based on 16S rRNA gene revealed that all strains recognized species Campylobacter (Zanoni, 2009). et al. Routine detection of species in clinical laboratories Campylobacter is on culture on selective media and subsequent phenotypic identification. Culture methods are based toward the detection of C. jejuni and The antimicrobial agents incorporated into used selective C. coli. media may inhibit growth of some species (Collins, Campylobacter et al. 2001 and Reddington, 2011). et al. The true incidence of species may be under Campylobacter estimated because of the limitations of routine culture methods because conventional methods are relatively slow (Engberg, 2000). et al. Presumptive results may be available after 2 days. However, definitive species-level identification based on phenotypic methods may require a further 3 to 4 days (O'Connor, 2000). Phenotypic identification can et al. be challenging because of the fastidious growth requirements and the distinguishing of biochemical characteristics by species Campylobacter (Goossens, & Butzler, 1992). Molecular methods on PCR amplification may provide an alternative to culture methods for the detection of in different Campylobacter specimens. The application of PCR-based assays applied to the detection of species in rabbit specimens (Maher, et al. 2003). Campylobacter Amplification of DNA targets including the flagellin Campylobacter gene, 16S rRNA and the 16S/23S rRNA intergenic spacer region (Touzet, 2009). et al.
unsheathed flagella, with no periplasmic fibres, and appeared coccoid after 10-12 days of incubation. Phylogenetic analyses based on 16S rRNA gene revealed that all strains recognized Campylobacter species (Zanoni, et al. 2009). Routine detection of Campylobacter species in clinical laboratories is on culture on selective media and subsequent phenotypic identification. Culture methods are based toward the detection of C. jejuni and C. coli. The antimicrobial agents incorporated into used selective media may inhibit growth of some Campylobacter species (Collins, et al. 2001 and Reddington, et al. 2011). The true incidence of Campylobacter species may be under estimated because of the limitations of routine culture methods because conventional methods are relatively slow (Engberg, et al. 2000). Presumptive results may be available after 2 days. However, definitive species-level identification based on phenotypic methods may require a further 3 to 4 days (O'Connor, et al. 2000). Phenotypic identification can be challenging because of the fastidious growth requirements and the distinguishing of biochemical characteristics by Campylobacter species (Goossens, & Butzler, 1992). Molecular methods on PCR amplification may provide an alternative to culture methods for the detection of Campylobacter in different specimens. The application of PCR-based assays applied to the detection of Campylobacter species in rabbit specimens (Maher, et al. 2003). Amplification of DNA targets including the Campylobacter flagellin gene, 16S rRNA and the 16S/23S rRNA intergenic spacer region (Touzet, et al. 2009).
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