Zhou T Definitely Definitely Definitely Definitely Definitely Definitely Definitely low risk low risk of low risk of low high risk of low risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - 3 - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of organoids culture, of data are recorded in cell cell media, blinding to provided for main text or suspension suspension solvent, study group each and by supplementary - ZIKV, - ZIKV, involving experiments titration material control control plate organoids, described author (5x10^5 software indicated PFU/ml) that investigators were not blinded to allocation during experiments and outcome assessment Table 4. Cellular effects of ZIKV according to lineage. or volume, cells Phospho-vimentin+) hours area, glia 72 size, Nestin+, after cells cells infectivity cells/radial organoid CASP3+) PAX6+, neurons phospho-H3) TUJ+) in replication Mature Viral Apoptosis Viability Preferential Progenitor (SOX2+, Proliferating Change Strains (TBR1+/TBR2+) (Ki67+, (TUNEL+, (EdU+) thickness Intermediate African Progenitor ↑ ↓ ↓ ↓ ↓ ↑ ↓ ↓ (MR766) cells > Mature 21 21 20 neurons 19,25
Zhou T Definitely low risk of bias - Homogenous cell suspension - ZIKV, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, control low risk of bias - Same media, solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Definitely low risk of bias - 3 organoids provided for each experiments Definitely low risk of bias - Viral culture, and titration described (5x10^5 PFU/ml) Definitely low risk of bias - Analysis of data by software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material Table 4. Cellular effects of ZIKV according to lineage. Strains Viral replication after 72 hours Preferential infectivity Progenitor cells/radial glia cells (SOX2+, PAX6+, Nestin+, Phospho-vimentin+) Intermediate cells (TBR1+/TBR2+) Proliferating cells (Ki67+, phospho-H3) Mature neurons TUJ+) Apoptosis (TUNEL+, CASP3+) Viability (EdU+) Change in organoid size, area, volume, or thickness African (MR766) ↑ Progenitor cells > Mature neurons 19,25 ↓ ↓ 21 ↓ ↓ 21 ↑ ↓ 20 ↓
Bayu.Sutarjono-RLRA.docx