Table X. Identity, function, origin, accession number, reference and location of the introduced genetic material for plasmid pMock808 Genetic Function of the DNA region Source organism Gene Reference element Accession number Right DNA region containing right border Agrobacterium Depicker et al., 1982 Border sequence used for transfer of the T-DNA tumefaciens Intervening Sequence used in DNA cloning Escherichia coli sequence Terminator and polyadenylation signal Bevan et al. 1983, nos 3’ Agrobacterium (see above). tumefaciens Depicker et al. 1982 cry1X1 Insect resistance gene. Bacillus thuringiensis MOCK123 (Adams 2001) Intervening Sequence used in DNA cloning Escherichia coli sequence e-35S Promoter with duplicated enhancer Cauliflower mosaic (Kay et al. 1987; Odell et region virus al. 1985) Intervening Sequence used in DNA cloning Escherichia coli sequence aad Antibiotic resistance marker gene Escherichia coli X04555 (Fling et al. 1985) containing its own regulatory sequences, ie promoter and termination sequences. Intervening Sequence used in DNA cloning Escherichia coli sequence nos 3’ Terminator and polyadenylation signal Agrobacterium As above. (see tumefaciens nptII Antibiotic resistance marker gene. Escherichia coli M61152 (Beck et al. 1982) e-35S Promoter (see above). Cauliflower mosaic As above. virus Intervening Sequence used in DNA cloning Escherichia coli sequence Left Border DNA region containing left border Agrobacterium Depicker et al., 1982 sequence used for transfer of the T-DNA tumefaciens Intervening Sequence used in DNA cloning Escherichia coli sequence ori-V Origin of replication for maintenance of Agrobacterium Stalker et al. 1981 plasmid in Agrobacterium tumefaciens Intervening Sequence used in DNA cloning Escherichia coli sequence ori-322 Origin of replication for maintenance of Escherichia coli Sutcliffe 1979 plasmid in E. coli Intervening Sequence used in DNA cloning Escherichia coli sequence
Table X. Identity, function, origin, accession number, reference and location of the introduced genetic material for plasmid pMock808 Genetic element Function of the DNA region Source organism Gene Accession number Reference Right Border DNA region containing right border sequence used for transfer of the T-DNA Agrobacterium tumefaciens Depicker et al., 1982 Intervening sequence Sequence used in DNA cloning Escherichia coli nos 3’ Terminator and polyadenylation signal (see above). Agrobacterium tumefaciens Bevan et al. 1983, Depicker et al. 1982 cry1X1 Insect resistance gene. Bacillus thuringiensis MOCK123 (Adams 2001) Intervening sequence Sequence used in DNA cloning Escherichia coli e-35S Promoter with duplicated enhancer region Cauliflower mosaic virus (Kay et al. 1987; Odell et al. 1985) Intervening sequence Sequence used in DNA cloning Escherichia coli aad Antibiotic resistance marker gene containing its own regulatory sequences, ie promoter and termination sequences. Escherichia coli X04555 (Fling et al. 1985) Intervening sequence Sequence used in DNA cloning Escherichia coli nos 3’ Terminator and polyadenylation signal (see Agrobacterium tumefaciens As above. nptII Antibiotic resistance marker gene. Escherichia coli M61152 (Beck et al. 1982) e-35S Promoter (see above). Cauliflower mosaic virus As above. Intervening sequence Sequence used in DNA cloning Escherichia coli Left Border DNA region containing left border sequence used for transfer of the T-DNA Agrobacterium tumefaciens Depicker et al., 1982 Intervening sequence Sequence used in DNA cloning Escherichia coli ori-V Origin of replication for maintenance of plasmid in Agrobacterium Agrobacterium tumefaciens Stalker et al. 1981 Intervening sequence Sequence used in DNA cloning Escherichia coli ori-322 Origin of replication for maintenance of plasmid in E. coli Escherichia coli Sutcliffe 1979 Intervening sequence Sequence used in DNA cloning Escherichia coli
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