extraction of DNA V01 10 4 1-4 2 Final Visit/Visit 02 Number of Tubes Tubes Tubes Processing/ Shipped Sample Type Tube Type Shipped to Retained Supplied in Aliquoting to Local Coriell at Site Kit Lab 2.5 ml for RNA PAXgeneTM 3 N/A 2 1 0 extraction Tube 2 ml 1 ml plasma aliquots microcentrifuge 18 in each 2 ml 9 0-9 0 Whole-blood: microcentrifuge tube for isolation of plasma/pellet 10ml Lavender Retain blood pellet in 3 2 1 0 Top EDTA Tube EDTA tube 50 ml conical Combine and mix 1 Tube total CSF N/A 15 ml conical Divide and spin 2 tubes CSF 2 ml 1 ml CSF aliquots in CSF microcentrifuge 18 each 2 ml 10 0-8 0 tubes microcentrifuge tube 2 ml purple-top 1 ml CSF aliquots in microcentrifuge 2 each 2 ml purple-top 0 0 2 tubes microcentrifuge tube TOTAL V02 47 23 2-19 2 The following samples will be collected according to the visit schedule noted above. Blood for standard clinical safety analysis to isolate plasma suitable for proteomic, metabolomic and other analyte studies and pellets of cells for protein analyses. Whole blood for appropriate DNA and RNA analysis Cerebrospinal fluid
extraction of DNA V01 10 4 1-4 2 Final Visit/Visit 02 Sample Type Tube Type Number of Tubes Supplied in Kit Processing/ Aliquoting Tubes Shipped to Coriell Retained at Site Tubes Shipped to Local Lab for RNA extraction 2.5 ml PAXgeneTM Tube 3 N/A 2 1 0 Whole-blood: for isolation of plasma/pellet 2 ml microcentrifuge 18 1 ml plasma aliquots in each 2 ml microcentrifuge tube 9 0-9 0 10ml Lavender Top EDTA Tube 3 Retain blood pellet in EDTA tube 2 1 0 CSF 50 ml conical Tube 1 Combine and mix total CSF N/A 15 ml conical tubes 2 Divide and spin CSF 2 ml microcentrifuge tubes 18 1 ml CSF aliquots in each 2 ml microcentrifuge tube 10 0-8 0 2 ml purple-top microcentrifuge tubes 2 1 ml CSF aliquots in each 2 ml purple-top microcentrifuge tube 0 0 2 TOTAL V02 47 23 2-19 2 The following samples will be collected according to the visit schedule noted above. Blood for standard clinical safety analysis to isolate plasma suitable for proteomic, metabolomic and other analyte studies and pellets of cells for protein analyses. Whole blood for appropriate DNA and RNA analysis Cerebrospinal fluid
BioFIND_Laboratory_Manual.docx