the cell pellets on dry ice (these will be stored at -80C until the next meeting). on ER2566 genotype: iron uptake deficiency (gene adjacent to McrBC) fhuA2 lon mutant in lon protease (activator of cell proteases) – – stabilizes foreign proteins ompT - mutant in outer membrane protease VII stabilizes – foreign proteins su inducible T7 RNA polymerase gene lacZ::T7 gene1 –IPTG – needed for pTZB1 expression sulA11 mutant in a negative regulator of cell division – (promotes growth in lon mutant background) transposable element mutation to R(mcr-73::miniTn10--TetS)2 mcrA endonuclease nuclease stabilizes foreign DNA – Complex mutation of the McrC and Δ(mcrC-mrr)114::IS10). Mrr endonuclease genes stabilizes foreign DNA – endA1 mutation in the endA endonuclease stabilizes foreign – DNA transposable element mutation [dcm] R(zgb-210::Tn10--TetS) – of dcm methylase
the cell pellets on dry ice (these will be stored at -80C until the next meeting). on ER2566 genotype: fhuA2 iron uptake deficiency (gene adjacent to McrBC) lon – mutant in lon protease (activator of cell proteases) – stabilizes foreign proteins ompT - mutant in outer membrane protease VII – stabilizes foreign proteins su lacZ::T7 gene1 –IPTG inducible T7 RNA polymerase gene – needed for pTZB1 expression sulA11 – mutant in a negative regulator of cell division (promotes growth in lon mutant background) R(mcr-73::miniTn10--TetS)2 transposable element mutation to mcrA endonuclease nuclease – stabilizes foreign DNA Δ(mcrC-mrr)114::IS10). Complex mutation of the McrC and Mrr endonuclease genes – stabilizes foreign DNA endA1 – mutation in the endA endonuclease stabilizes foreign DNA [dcm] R(zgb-210::Tn10--TetS) – transposable element mutation of dcm methylase
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