Conjugates dilution T2–QDs SAM 1/20 1/75 1/50 1/45 1/80 1/50 1/40 DAM FLISA – – – GBA 1/15 1/50 1/25 1/30 1/60 Analytical characteristics for mycotoxins’ determination by ELISA and Table SI-2. multiplex FLISA in buffer solutions (n = 5). Linear range, ng LOD, ng mL-1 IC50, ng mL-1 mL-1 ELISA FLISA ELISA ELISA FLISA 1.4 0.16 27±2 11±1.4 6.5-80 2-29 DON 0.2 0.03 1.5±0.3 0.13±0.03 ZEN 0.12 0.01 1.34±0.05 0.11±0.02 AfB1 5 0.5 62±5 9±1.1 16-160 2-24 T2 0.2 0.02 2.4±0.07 0.3±0.06 0.6-5.9 FB1 Analytical performance of the column gel–based immunoassay. Table SI–3. Value, % Parameter DON ZEN AfB1 T2 FB1 rate, % 2.1 1.9 1.4 2.6 2.4 (Nfalse positive/N−) ×100) False–negative rate, % 1.5 2.2 1.2 2.8 2.0 (Nfalse negative/N+) ×100) 97.6 96.0 98.5 97.4 96.8 Specificity rate, %
Conjugates dilution T2–QDs SAM 1/20 1/75 1/50 1/45 1/80 DAM FLISA – 1/50 1/40 – – GBA 1/15 1/50 1/25 1/30 1/60 Table SI-2. Analytical characteristics for mycotoxins’ determination by ELISA and multiplex FLISA in buffer solutions (n = 5). LOD, ng mL-1 IC50, ng mL-1 Linear range, ng mL-1 ELISA FLISA ELISA ELISA FLISA DON 1.4 0.16 27±2 11±1.4 6.5-80 2-29 ZEN 0.2 0.03 1.5±0.3 0.13±0.03 AfB1 0.12 0.01 1.34±0.05 0.11±0.02 T2 5 0.5 62±5 9±1.1 16-160 2-24 FB1 0.2 0.02 2.4±0.07 0.3±0.06 0.6-5.9 Table SI–3. Analytical performance of the column gel–based immunoassay. Parameter Value, % DON ZEN AfB1 T2 FB1 rate, % (Nfalse positive/N−) ×100) 2.1 1.9 1.4 2.6 2.4 False–negative rate, % (Nfalse negative/N+) ×100) 1.5 2.2 1.2 2.8 2.0 Specificity rate, % 97.6 96.0 98.5 97.4 96.8
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