method not reported Probably Probably Definitely Probably Definitely Definitely Definitely low risk CQ high risk of high risk of low low risk of high risk of low risk of low risk of of bias bias bias risk of bias bias (not bias bias - All measured - - bias - recorded) - Viral - Analysis outcomes outlined Homogenous Homogenous - Same - culture, of data are recorded in cell cell media, assays Insufficient main text or suspension suspension solvent, kept blind information and by supplementary - ZIKV, no - ZIKV, no provided titration Harmony material (only ZIKV controls controls plate about described 5.1 number of (MOI = 1 or software reported) organoids 10) (number of organoids not recorded) Definitely Definitely Definitely Definitely Definitely Definitely Definitely low risk M low risk of low risk of low high risk of low risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - Number of - - Analysis outcomes outlined Homogenous Homogenous - Same evidence of organoids culture, of data are recorded in cell cell media, blinding to provided for main text or suspension suspension solvent, study group all and by supplementary - ZIKV, - ZIKV, involving experiments titration ImageJ or material control control plate organoids, described author (MOI = 0.3 indicated or 3.125) software that investigators were not blinded to allocation during experiments and outcome assessment Wells MF Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low high risk of low risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of Insufficient culture, of data are recorded in cell cell blinding to information main text or suspension suspension solvent, study group provided and by supplementary - ZIKV-PR, - ZIKV-PR, involving about titration ImageJ material control control plate organoids, number of described software author organoids (4 (MOI = 0.1, indicated or 6 1, or 10) that organoids investigators used) were not **Ki67 blinded to allocation not during quantified experiments
method not reported CQ Probably high risk of bias - Homogenous cell suspension - ZIKV, no controls Probably high risk of bias - Homogenous cell suspension - ZIKV, no controls low risk of bias - Same media, solvent, plate Definitely low risk of bias - assays kept blind Probably high risk of bias (not recorded) - Insufficient information provided about number of organoids (number of organoids not recorded) Definitely low risk of bias - Viral culture, and titration described (MOI = 1 or 10) Definitely low risk of bias - Analysis of data by Harmony 5.1 software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material (only ZIKV reported) M Definitely low risk of bias - Homogenous cell suspension - ZIKV, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, control low risk of bias - Same media, solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Definitely low risk of bias - Number of organoids provided for all experiments Definitely low risk of bias - culture, and titration described (MOI = 0.3 or 3.125) Definitely low risk of bias - Analysis of data by ImageJ or software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material Wells MF Definitely low risk of bias - Homogenous cell suspension - ZIKV-PR, control Definitely low risk of bias - Homogenous cell suspension - ZIKV-PR, control low risk of bias - Same solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments Probably low risk of bias - Insufficient information provided about number of organoids (4 or 6 organoids used) **Ki67 not quantified Definitely low risk of bias - Viral culture, and titration described (MOI = 0.1, 1, or 10) Definitely low risk of bias - Analysis of data by ImageJ software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material
Bayu.Sutarjono-RLRA.docx