gall bladder. It helps in Interpret graphs to determine Discuss the role of bile and the demos and up liquid, tube of the digestion of fats by the optimum temperature or demonstrate the action of discuss the outcomes. water and olive oil neutralising acid from pH for an enzyme. washing up liquid on fats. Explain why pH is Demo: two tubes full the stomach and measured to indicate the Demonstrate the effect of bile Carry out other enzyme cream milk, sodium emulsifying fats. rate of fat digestion. salts on the rate of digestion of controlled investigations as carbonate solution, milk using a pH indicator. Different enzymes appropriate. Investigate the optimum phenolphthalein or work best at different pH values for pepsin UI solution, lipase Calculate the rate of enzyme Using a model or large poster temperatures and pH and trypsin enzymes. solution, +/- washing controlled reactions. of the digestive system identify values. up liquid and timer. Relate the results to where each type of enzyme Interpret the results from their sites of action in pH: Pepsin solution, and bile is produced. enzyme controlled reactions. the digestive system. trypsin solution, Add labels to the digestive buffer solutions at system diagram to show where Investigate the effect of Required practical: different pH values, the different enzymes and bile temperature on amylase Food UI strips, egg white are produced. activity measure time – suspension, test Use qualitative taken for starch to tubes, timers and Optional investigations or use reagents to test for a disappear. Plot results goggles. computer simulations and range of and find optimum interpret and explain the carbohydrates, lipids temperature for Amylase: amylase results: and proteins. To amylase. solution, starch include: Benedict’s test Investigate the solution, test Plot and interpret graphs for sugars; iodine test optimum pH values for water baths at about enzyme activity; for starch; and Biuret pepsin and trypsin. different determine optimal reagent for protein. Investigate the effect temperatures, temperatures and pH thermometers, glass of temperature on values. rods, spotting tiles, amylase activity. iodine solution, timers, goggles.
gall bladder. It helps in the digestion of fats by neutralising acid from the stomach and emulsifying fats. Different enzymes work best at different temperatures and pH values. Required practical: Food Use qualitative reagents to test for a range of carbohydrates, lipids and proteins. To include: Benedict’s test for sugars; iodine test for starch; and Biuret reagent for protein. Interpret graphs to determine the optimum temperature or pH for an enzyme. Carry out other enzyme controlled investigations as appropriate. Calculate the rate of enzyme controlled reactions. Interpret the results from enzyme controlled reactions. Discuss the role of bile and demonstrate the action of washing up liquid on fats. Demonstrate the effect of bile salts on the rate of digestion of milk using a pH indicator. Using a model or large poster of the digestive system identify where each type of enzyme and bile is produced. Add labels to the digestive system diagram to show where the different enzymes and bile are produced. Optional investigations or use computer simulations and interpret and explain the results: Investigate the optimum pH values for pepsin and trypsin. Investigate the effect of temperature on amylase activity. the demos and discuss the outcomes. Explain why pH is measured to indicate the rate of fat digestion. Investigate the optimum pH values for pepsin and trypsin enzymes. Relate the results to their sites of action in the digestive system. Investigate the effect of temperature on amylase activity – measure time taken for starch to disappear. Plot results and find optimum temperature for amylase. Plot and interpret graphs about enzyme activity; determine optimal temperatures and pH values. up liquid, tube of water and olive oil Demo: two tubes full cream milk, sodium carbonate solution, phenolphthalein or UI solution, lipase solution, +/- washing up liquid and timer. pH: Pepsin solution, trypsin solution, buffer solutions at different pH values, UI strips, egg white suspension, test tubes, timers and goggles. Amylase: amylase solution, starch solution, test water baths at different temperatures, thermometers, glass rods, spotting tiles, iodine solution, timers, goggles.
AQA-8464-SOW-ORG.DOCX