respectively. A longer exposure of Fig. 2C. (B) Stability of pJH3.0 transformants passaged up to 30 times with antibiotic resistance. Southern blot analysis of uncut genomic DNA from untransformed (PG45), transformed with pJH3.0 M. bovis M. bovis after 10 (P10), 20 (P20), and 30 (P30) passages with different incubation time, pJH3.0 plasmid was used as control. (C) Stability of pJHmini3.0 transformants passaged up to 30 times with antibiotic resistance. Southern blot analysis of uncut genomic DNA from untransformed (PG45), transformed with pJHmini3.0 after 10 (P10), 20 M. bovis M. bovis (P20), and 30 (P30) passages with different incubation time, pJHmini3.0 plasmid was used as control. Visualization was performed with ethidium bromide (EtBr) and a fragment was used as a probe. tetM Figure S5 Reproducibility of pJHmini3.0 plasmid Figure S5 Reproducibility of pJHmini3.0 plasmid. Plasmid DNA extracted from untransformed (PG45) and transformed pJHmini3.0 after 10 (P10), 20 M. bovis M. bovis (P20), and 30 (P30) passages was analysed by electrophoresis along with pJHmini3.0 plasmid. Figure S6 Conserved DNA sequence flanking region between and oriC M. bovis M. agalactiae Figure S6 (A) Alignment of DNA sequence flanking region between and oriC M. bovis its closely related species using DNAMAN software. Upper line: Mycoplasma agalactiae sequence of (GenBank: CP002188.1 from 1,141 to 1,859), lower line: sequence M. bovis
respectively. A longer exposure of Fig. 2C. (B) Stability of pJH3.0 transformants passaged up to 30 times with antibiotic resistance. Southern blot analysis of uncut genomic DNA from untransformed M. bovis (PG45), M. bovis transformed with pJH3.0 after 10 (P10), 20 (P20), and 30 (P30) passages with different incubation time, pJH3.0 plasmid was used as control. (C) Stability of pJHmini3.0 transformants passaged up to 30 times with antibiotic resistance. Southern blot analysis of uncut genomic DNA from untransformed M. bovis (PG45), M. bovis transformed with pJHmini3.0 after 10 (P10), 20 (P20), and 30 (P30) passages with different incubation time, pJHmini3.0 plasmid was used as control. Visualization was performed with ethidium bromide (EtBr) and a tetM fragment was used as a probe. Figure S5 Reproducibility of pJHmini3.0 plasmid Figure S5 Reproducibility of pJHmini3.0 plasmid. Plasmid DNA extracted from untransformed M. bovis (PG45) and M. bovis transformed pJHmini3.0 after 10 (P10), 20 (P20), and 30 (P30) passages was analysed by electrophoresis along with pJHmini3.0 plasmid. Figure S6 Conserved DNA sequence flanking oriC region between M. bovis and M. agalactiae Figure S6 (A) Alignment of DNA sequence flanking oriC region between M. bovis and its closely related species Mycoplasma agalactiae using DNAMAN software. Upper line: sequence of M. bovis (GenBank: CP002188.1 from 1,141 to 1,859), lower line: sequence
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