usually present in large amounts, veterinary samples are not usually treated with trypsin. Trypsin may be added to the suspension at the same time as the broth or saline is added. REFERENCES Carter,G.R and Cole,J.R. Jr. Diagnostic Procedures in Veterinary Bacteriology and Mycology, 1990. Academic Press. ISBN 0-12-161775-0. Donahue,J.M. Anaerobic Bacteria, In: Diagnostic Procedures in Veterinary Bacteriology and Mycology (fifth ed.) 1990, edited by G.R.Carter and J.R.Cole, Jr. Academic Press. ISBN 0-12-161775-0. Quinn,P.J; Carter,M.E; Markey,B & Carter, G.R. Non-spore-forming Anaerobic Bacteria, In Clinical Veterinary Microbiology,1994.Wolfe Publishing.ISBN 0 7234 1711 3. Willis, A.T. Anaerobic Bacteriology: Clinical and Laboratory Practice (third edition),1977; Butterworths and Co.; ISBN0-407-00081-X. Palmer MA (1993) A gelatin test to detect activity and stability of proteases produced by (bacteroides) Veterinary Microbiology 36:113-122 Dichelobacter nodosus Stewart DJ, Peterson JE, Vaughan JA, BL, Emery DL, Caldwell JB and Kort AA (1986b) The pathogenicity and cultural characteristics of virulent, intermediate and benign strains of Bacteroides nodosus causing ovine footrot. Australian Veterinary Journal 63:317-326 APPENDIX: MEDIA AND REAGENTS FOR THE ISOLATION OF ANAEROBIC BACTERIA VL (Viande-Levure) broth This is a most useful basal broth from which an almost complete range of fluid and solid media Tryptone 10g. for the identification of anaerobes can be NaCl 5g. Beef extract 2g. prepared. Yeast extract 5g. Cysteine HCl 0,4g Agar 0,6g Sugars (filter sterilised 10% solutions of the Distilled water. 1000ml various sugars) are added after the broth has been autoclaved. Heat to dissolve, adjust pH to 7,2-7,4, dispense, sterilise by autoclaving at 115°C for 10 minutes.
usually present in large amounts, veterinary samples are not usually treated with trypsin. Trypsin may be added to the suspension at the same time as the broth or saline is added. REFERENCES Carter,G.R and Cole,J.R. Jr. Diagnostic Procedures in Veterinary Bacteriology and Mycology, 1990. Academic Press. ISBN 0-12-161775-0. Donahue,J.M. Anaerobic Bacteria, In: Diagnostic Procedures in Veterinary Bacteriology and Mycology (fifth ed.) 1990, edited by G.R.Carter and J.R.Cole, Jr. Academic Press. ISBN 0-12-161775-0. Quinn,P.J; Carter,M.E; Markey,B & Carter, G.R. Non-spore-forming Anaerobic Bacteria, In Clinical Veterinary Microbiology,1994.Wolfe Publishing.ISBN 0 7234 1711 3. Willis, A.T. Anaerobic Bacteriology: Clinical and Laboratory Practice (third edition),1977; Butterworths and Co.; ISBN0-407-00081-X. Palmer MA (1993) A gelatin test to detect activity and stability of proteases produced by Dichelobacter (bacteroides) nodosus Veterinary Microbiology 36:113-122 Stewart DJ, Peterson JE, Vaughan JA, BL, Emery DL, Caldwell JB and Kort AA (1986b) The pathogenicity and cultural characteristics of virulent, intermediate and benign strains of Bacteroides nodosus causing ovine footrot. Australian Veterinary Journal 63:317-326 APPENDIX: MEDIA AND REAGENTS FOR THE ISOLATION OF ANAEROBIC BACTERIA VL (Viande-Levure) broth Tryptone NaCl Beef extract Yeast extract Cysteine HCl Agar Distilled water. 10g. 5g. 2g. 5g. 0,4g 0,6g 1000ml Heat to dissolve, adjust pH to 7,2-7,4, dispense, sterilise by autoclaving at 115°C for 10 minutes. This is a most useful basal broth from which an almost complete range of fluid and solid media for the identification of anaerobes can be prepared. Sugars (filter sterilised 10% solutions of the various sugars) are added after the broth has been autoclaved.
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