Dose (milligrams per Route Comments kilogram) Cervical dislocation N/A N/A Acceptable with prior sedation or anesthesia; exceptions must be approved by IACUC Decapitation N/A N/A Acceptable with prior sedation or anesthesia; if unable to sedate or anesthetize because need chemical residue-free tissues, head should be immediately frozen in liquid nitrogen subsequent to severing; exceptions must be approved by IACUC Exsanguinations N/A N/A Acceptable when preceded by methods that relieve anxiety, consciousness RATS Tranquilizers Dose (milligrams per Agent Comments kilogram) Telazol (Tiletamine 40 IP For chemical restraint; for light HCl/Zolazepam HCl) 20-60 IM anesthesia Acetylpromazine 0.5 IM (Acepromazine) Diazepam (Valium) 3-5 IM/IP Ketamine (Ketaset, Vetalar) 50-100 IM/IP General Anesthetics Dose (milligrams per Agent Route Comments kilogram) Choralose 55 IP Scientific justification needed; terminal studies only Ketamine/Xylazine (Rompun) Ketamine 40-80 mg IM/IP Make a mixture by adding 0.15 ml mixture Xylazine 5-10 mg Xylazine (100 mg/ml stock solution) to each ml of ketamine (100 mg/ml stock solution); maximal anesthetic effect is achieved within 15 minutes and lasts 15-30 minutes Mixtures with Diazepam and Ketamine 40-60 IP Make a mixture by adding 0.15 ml Ketamine Diazepam 5-10 Xylazine (100 mg/ml stock solution) to each ml of ketamine (100 mg/ml stock solution); maximal anesthetic effect is achieved within 15 minutes and lasts 15-30 minutes Telazol (Tiletamine HCl, 20-60 IM For chemical restraint; for light Zolazepam HCl) 40 IP anesthesia Thiopental (Pentothal) 20 IV For chemical restraint 40 IP
Dose (milligrams per kilogram) Route Comments Cervical dislocation N/A N/A Acceptable with prior sedation or anesthesia; exceptions must be approved by IACUC Decapitation N/A N/A Acceptable with prior sedation or anesthesia; if unable to sedate or anesthetize because need chemical residue-free tissues, head should be immediately frozen in liquid nitrogen subsequent to severing; exceptions must be approved by IACUC Exsanguinations N/A N/A Acceptable when preceded by methods that relieve anxiety, consciousness RATS Tranquilizers Agent Dose (milligrams per kilogram) Comments Telazol (Tiletamine HCl/Zolazepam HCl) 40 20-60 IP IM For chemical restraint; for light anesthesia Acetylpromazine (Acepromazine) 0.5 IM Diazepam (Valium) 3-5 IM/IP Ketamine (Ketaset, Vetalar) 50-100 IM/IP General Anesthetics Agent Dose (milligrams per kilogram) Route Comments Choralose 55 IP Scientific justification needed; terminal studies only Ketamine/Xylazine (Rompun) mixture Ketamine 40-80 mg Xylazine 5-10 mg IM/IP Make a mixture by adding 0.15 ml Xylazine (100 mg/ml stock solution) to each ml of ketamine (100 mg/ml stock solution); maximal anesthetic effect is achieved within 15 minutes and lasts 15-30 minutes Mixtures with Diazepam and Ketamine Ketamine 40-60 Diazepam 5-10 IP Make a mixture by adding 0.15 ml Xylazine (100 mg/ml stock solution) to each ml of ketamine (100 mg/ml stock solution); maximal anesthetic effect is achieved within 15 minutes and lasts 15-30 minutes Telazol (Tiletamine HCl, Zolazepam HCl) 20-60 40 IM IP For chemical restraint; for light anesthesia Thiopental (Pentothal) 20 40 IV IP For chemical restraint
ARF-standard-operating-procedures.docx