3) Stability to Alkali: Amino acids, unlike amides and volatile amines, do not evolve NH3 or alkaline vapor when boiled with alkali. This method can be used to differentiate amino acids from amines and amides. Apply this test to the provided amine or amide and also to glycine. Procedure: - Pipette 1 mL 1% glycine and the amide or amine solution into separate test tubes. - Add 1 mL dilute NaOH to each test tube and boil. - Test the from each boiling tube with wet litmus paper. Questions: What type of reaction is responsible for the evolution of alkaline Write the reaction and explain briefly. 4) Specific Reactions for Individual Acids: WARNING: Please DO NOT use vast amounts of solution for tests, since most of the amino acids are very expensive!! a) Xanthoproteic Test: Some acids contain aromatic groups that are derivatives of benzene. These aromatic groups can undergo reactions that are characteristics of benzene and benzene derivatives.
3) Stability to Alkali: Amino acids, unlike amides and volatile amines, do not evolve NH3 or alkaline vapor when boiled with alkali. This method can be used to differentiate amino acids from amines and amides. Apply this test to the provided amine or amide and also to glycine. Procedure: - Pipette 1 mL 1% glycine and the amide or amine solution into separate test tubes. - Add 1 mL dilute NaOH to each test tube and boil. - Test the from each boiling tube with wet litmus paper. Questions: What type of reaction is responsible for the evolution of alkaline Write the reaction and explain briefly. 4) Specific Reactions for Individual Acids: WARNING: Please DO NOT use vast amounts of solution for tests, since most of the amino acids are very expensive!! a) Xanthoproteic Test: Some acids contain aromatic groups that are derivatives of benzene. These aromatic groups can undergo reactions that are characteristics of benzene and benzene derivatives.
Biochem%20Lab%20Manual.docx