PRIMARY method(s) of euthanasia CO2 by compressed gas cylinder (Not for animals already under anesthesia or neonates) Barbiturate Euthanasia Solution - Injectable (Check route below): ≥150mg/kg IV IP IC Isoflurane Anesthesia Overdose - Inhalant Cervical Dislocation (only under anesthesia) Decapitation (only anesthesia or neonates) Exsanguination or Perfusion (only under anesthesia) Incision of Chest Cavity Bilateral Pneumothorax (only under anesthesia) – Pithing (only under anesthesia) ( amphibians, reptiles only) – Removal of Vital Organ(s) (only under anesthesia) (Check all that apply): Brain Kidneys GI Tract Liver Lungs Other Vital Organ(s) (Specify): – Other Method of Euthanasia: (Describe and Scientifically Justify): SECONDARY method(s) of euthanasia that will be used to ensure that the animal does not survive: Cervical Dislocation Decapitation Exsanguination or Perfusion Incision of Chest Cavity Bilateral Pneumothorax – Barbiturate Euthanasia Solution - Injectable (Check route below): ≥150mg/kg IV IP IC Pithing Double pithing required (fish, amphibians, reptiles only) – Removal of Vital Organ(s): (Check all that apply): Brain Kidneys Heart GI Tract Liver Lungs Vital Organ(s) (Specify): – Other Method of Euthanasia: (Describe and Scientifically Justify):
PRIMARY method(s) of euthanasia CO2 by compressed gas cylinder (Not for animals already under anesthesia or neonates) Barbiturate Euthanasia Solution - Injectable ≥150mg/kg (Check route below): IV IP IC Isoflurane Anesthesia Overdose - Inhalant Cervical Dislocation (only under anesthesia) Decapitation (only anesthesia or neonates) Exsanguination or Perfusion (only under anesthesia) Incision of Chest Cavity – Bilateral Pneumothorax (only under anesthesia) Pithing – (only under anesthesia) ( amphibians, reptiles only) Removal of Vital Organ(s) (only under anesthesia) (Check all that apply): Brain Kidneys GI Tract Liver Lungs Other Vital Organ(s) – (Specify): Other Method of Euthanasia: (Describe and Scientifically Justify): SECONDARY method(s) of euthanasia that will be used to ensure that the animal does not survive: Cervical Dislocation Decapitation Exsanguination or Perfusion Incision of Chest Cavity – Bilateral Pneumothorax Barbiturate Euthanasia Solution - Injectable ≥150mg/kg (Check route below): IV IP IC Pithing – Double pithing required (fish, amphibians, reptiles only) Removal of Vital Organ(s): (Check all that apply): Brain Kidneys Heart GI Tract Liver Lungs Vital Organ(s) – (Specify): Other Method of Euthanasia: (Describe and Scientifically Justify):
ApplicationToUseAnimals-022016.docx