Gabriel E Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low high risk of high risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - Evidence - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of of all culture, of data are recorded in cell cell media, blinding to organoids main text or suspension suspension solvent, study group used was and by supplementary - ZIKV-AS, - ZIKV-AS, involving documented titration ImageJ material ZIKV-AM, ZIKV-AM, plate organoids, by table S2 described software ZIKV-AF, ZIKV-AF, author **Cortical (MOI = 1) Control Control indicated plate that thickness investigators measured were not only for blinded to ZIKV-AS, not allocation ZIKV-AM or during ZIKV-AF experiments **Values and outcome not shown assessment for TUNEL assay **Organoid diameter measured for ZIKV-AF only, not ZIKV-AS or ZIKV-AM of ventricular zone lumen exhibiting cells measured for ZIKV-AS and not ZIKV-AF Garcez P Definitely Definitely Definitely Probably Definitely Definitely Definitely low risk low risk of low risk of low high risk of low risk of low risk of low risk of of bias bias bias risk of bias bias bias bias - All measured - - bias - No - - Viral - Analysis outcomes outlined Homogenous Homogenous - Same evidence of Insufficient culture, of data are recorded in cell cell media, blinding to information main text or suspension suspension solvent, group provided and by supplementary - ZIKV, - ZIKV, involving about titration Harmony material DENV2, DENV2, plate organoids, number of described 5.1 control control author organoids (3 (MOI = software indicated or 6 0.025 to that organoids 0.25) investigators used) were not blinded to allocation during experiments and outcome assessment
Gabriel E Definitely low risk of bias - Homogenous cell suspension - ZIKV-AS, ZIKV-AM, ZIKV-AF, Control Definitely low risk of bias - Homogenous cell suspension - ZIKV-AS, ZIKV-AM, ZIKV-AF, Control low risk of bias - Same media, solvent, plate Definitely high risk of bias - No evidence of blinding to study group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Probably high risk of bias - Evidence of all organoids used was documented by table S2 **Cortical plate thickness measured only for ZIKV-AS, not ZIKV-AM or ZIKV-AF **Values not shown for TUNEL assay **Organoid diameter measured for ZIKV-AF only, not ZIKV-AS or ZIKV-AM of ventricular zone lumen exhibiting cells measured for ZIKV-AS and not ZIKV-AF Definitely low risk of bias - Viral culture, and titration described (MOI = 1) Definitely low risk of bias - Analysis of data by ImageJ software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material Garcez P Definitely low risk of bias - Homogenous cell suspension - ZIKV, DENV2, control Definitely low risk of bias - Homogenous cell suspension - ZIKV, DENV2, control low risk of bias - Same media, solvent, plate Definitely high risk of bias - No evidence of blinding to group involving organoids, author indicated that investigators were not blinded to allocation during experiments and outcome assessment Probably low risk of bias - Insufficient information provided about number of organoids (3 or 6 organoids used) Definitely low risk of bias - Viral culture, and titration described (MOI = 0.025 to 0.25) Definitely low risk of bias - Analysis of data by Harmony 5.1 software Definitely low risk of bias - All measured outcomes outlined are recorded in main text or supplementary material
Bayu.Sutarjono-RLRA.docx