Supplementary 1: Table depicting primer sets used for PCR amplification in the study Primers Reference Primary PCR Wen et al, 1997 Ehrlichia canis ECC: 5’-AGAACGAACGCTGGCGGCAAGCC-3’ ECB:5’-CGTATTACCGCGGCTGCTGGC-3’ Nested PCR HE-3: 5’-TATAGGTACCGTCATTATCTTCCCTAT-3’ ECA:5’-CAATTATTTATAGCCTCTGGCTATAGGAA-3’ B18S-F: 5’-TGGTTGATCCTGCCAGTA-3’ Ikadai et al, 2004 species Babesia B18S-R: 5’-CTTCTCCTTCCTTTA AGTGA-3’ 5’-TGCAGACGACCTGACGCTACT-3’ Wuyts et al, 1994 Trypanosoma evansi 5’-CTCCTAGAAGCTTCGGTGTCCT -3’
Supplementary 1: Table depicting primer sets used for PCR amplification in the study Primers Reference Ehrlichia canis Primary PCR ECC: 5’-AGAACGAACGCTGGCGGCAAGCC-3’ ECB:5’-CGTATTACCGCGGCTGCTGGC-3’ Nested PCR HE-3: 5’-TATAGGTACCGTCATTATCTTCCCTAT-3’ ECA:5’-CAATTATTTATAGCCTCTGGCTATAGGAA-3’ Wen et al, 1997 Babesia species B18S-F: 5’-TGGTTGATCCTGCCAGTA-3’ B18S-R: 5’-CTTCTCCTTCCTTTA AGTGA-3’ Ikadai et al, 2004 Trypanosoma evansi 5’-TGCAGACGACCTGACGCTACT-3’ 5’-CTCCTAGAAGCTTCGGTGTCCT -3’ Wuyts et al, 1994
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