3.3.16 Chromatography Chromatography provides an important method of separating and identifying components in a mixture. Different types of chromatography are used depending on the composition of mixture to be separated Prior knowledge: AS level Chemistry - 3.3.13 Amino acids, proteins and DNA (this section requires use of thin-layer chromatography for analysis of amino acids it could be taught before or after – this section) Time Learning activity with opportunity to Assessment Learning outcome Resources Learning objective develop skills opportunities 0.6 AQA Chromatography Produce a summary to compare January 2011 Unit 4 Describe the similarities Students should be able to: weeks similarities and differences between Question 4f Teachers’ Notes: and differences between describe the similarities thin-layer, column and gas (QW11.4.04) thin-layer, column and gas and differences between chromatography (AO1 - Demonstrate column and gas chromatography. knowledge and understanding of chromatography RCS on TLC scientific ideas). explain how Separate mixtures and identify Explain how chromatography works Modern Chemical substances (eg amino acids) by chromatography Techniques RSC use retention times and Rf chromatography (AO2 - Apply values to identify resource: knowledge and understanding; AT i - substances Use chromatography to Use thin-layer or paper describe the use of mass chromatography). separate and identify spectroscopy to analyse Chemistry Review substances. Use retention time and Rf data to substances separated by articles: How pure is your identify substances separated by gas chromatography. chromatography.
3.3.16 Chromatography Chromatography provides an important method of separating and identifying components in a mixture. Different types of chromatography are used depending on the composition of mixture to be separated Prior knowledge: AS level Chemistry - 3.3.13 Amino acids, proteins and DNA (this section requires use of thin-layer chromatography for analysis of amino acids – it could be taught before or after this section) Learning objective Time Learning outcome Learning activity with opportunity to develop skills Assessment opportunities Resources Describe the similarities and differences between thin-layer, column and gas chromatography. Explain how chromatography Use chromatography to separate and identify substances. 0.6 weeks Students should be able to: describe the similarities and differences between column and gas chromatography explain how chromatography works use retention times and Rf values to identify substances describe the use of mass spectroscopy to analyse substances separated by gas chromatography. Produce a summary to compare similarities and differences between thin-layer, column and gas chromatography (AO1 - Demonstrate knowledge and understanding of scientific ideas). Separate mixtures and identify substances (eg amino acids) by chromatography (AO2 - Apply knowledge and understanding; AT i - Use thin-layer or paper chromatography). Use retention time and Rf data to identify substances separated by chromatography. January 2011 Unit 4 Question 4f (QW11.4.04) AQA Chromatography Teachers’ Notes: RCS on TLC Modern Chemical Techniques RSC resource: Chemistry Review articles: How pure is your
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