Feed consumption Composition of Diet Water Acclimation period Predosing (kg/day) Yes or No B.7.2.2-4. Test Animal Dosing Regime. Treatment Level Dose Treatment Type Vehicle Timing/Duration (ppm in diet)1 (mg ai/kg bw/day) capsule, feed, Oral bolus, etc. Dermal 1 Dietary burden on a dry-weight basis. Sampling Table B.7.2.2-5. Sample Collection Information. Feces, and Cage Wash Interval From Last Dose Milk Collected Tissues Harvested and Analyzed Collected* to Sacrifice XXX XXX daily XXX hours *If available. [Briefly describe how samples were taken, parts sampled, how samples were handled after collection (shipment, storage, etc.), and any preparation that was done prior to extraction.] Extraction and Analysis [If available, then include a flowchart of the extraction and fractionation schemes.] [Briefly describe the extraction, fractionation and hydrolysis strategies for each tissue including solvents used (ratios), the order of their use, the extraction procedures employed (i.e., blending, maceration, Soxhlet, etc.) and other extraction techniques.] [Briefly describe procedures used to release bound and conjugated residues (i.e., acid, base, or enzyme hydrolysis, exhaustive extraction, etc.). Has the petitioner justified the use of severe conditions (e.g., strong acid hydrolysis in the presence of heat, etc.)?] Identification and Characterization [Briefly describe the methods used for identification/characterization of the residues (LSC, TLC, GLC, HPLC, etc.). If applicable, then very briefly describe difficulties with methods that fail to elucidate the nature of the residues or bound residues.] II. RESULTS AND DISCUSSION A. Total Radioactive Residues Quantitation
Composition of Diet Feed consumption (kg/day) Water Acclimation period Predosing Yes or No B.7.2.2-4. Test Animal Dosing Regime. Treatment Type Treatment Level (ppm in diet)1 Vehicle Dose (mg ai/kg bw/day) Timing/Duration Oral capsule, feed, bolus, etc. Dermal 1 Dietary burden on a dry-weight basis. Sampling Table B.7.2.2-5. Sample Collection Information. Milk Collected Feces, and Cage Wash Collected* Interval From Last Dose to Sacrifice Tissues Harvested and Analyzed XXX XXX daily XXX hours *If available. [Briefly describe how samples were taken, parts sampled, how samples were handled after collection (shipment, storage, etc.), and any preparation that was done prior to extraction.] Extraction and Analysis [If available, then include a flowchart of the extraction and fractionation schemes.] [Briefly describe the extraction, fractionation and hydrolysis strategies for each tissue including solvents used (ratios), the order of their use, the extraction procedures employed (i.e., blending, maceration, Soxhlet, etc.) and other extraction techniques.] [Briefly describe procedures used to release bound and conjugated residues (i.e., acid, base, or enzyme hydrolysis, exhaustive extraction, etc.). Has the petitioner justified the use of severe conditions (e.g., strong acid hydrolysis in the presence of heat, etc.)?] Identification and Characterization [Briefly describe the methods used for identification/characterization of the residues (LSC, TLC, GLC, HPLC, etc.). If applicable, then very briefly describe difficulties with methods that fail to elucidate the nature of the residues or bound residues.] II. RESULTS AND DISCUSSION A. Total Radioactive Residues Quantitation
b.7.2_livestock_metabolism.docx