IN SMALL RUMINANTS IN THE REPUBLIC OF CROATIA BRUCELLA MELITENSIS Sanja Duvnjak1; Irena Reil1; Zeljko Cvetnic1; Silvio Spicic1; Tomislav Kiš 2; Maja Zdelar-Tuk1; Boris Habrun1; Miroslav Benic1; Gordan Kompes1 Scientific paper Summary In the period from 2009 to 2013, comprehensive melitococcosis testing was conducted in the Republic of Croatia. During the testing, the Rose Bengal test was applied to 344 019 blood samples of sheep and goats, and positive reactions were confirmed in 1143 (0.3%) of samples. The complement fixation test (confirmatory test) was conducted on 43 428 samples, with positive reactions confirmed in 768 (1.8%) of samples. The organs and tissues of 336 sheep and goats were inspected bacteriologically, and sp. was isolated in 15 (4.5%) of samples. Bacteriological and molecular techniques (Bru Brucella up/Bru-low and Bruce-Ladder) in isolates proved the presence of biovar 3. Brucella melitensis Key words: Brucella melitensis, ruminants, serological and bacteriological tests, Republic of Croatia INTRODUCTION Melitococcosis or brucellosis is a chronic infectious disease of sheep and goats caused by the species (B.) It is one of the most widespread zoonoses worldwide. This paper gives an Brucella melitensis. overview of the distribution of (B.) in the Republic of Croatia in the period from Brucella melitensis 2009 to 2013. Bacteriological and molecular techniques were used to prove and confirm the species sp. Brucella MATERIAL AND METHODS In the investigated period from 2009 to 2013, a total of 344 019 blood samples of sheep and goats were tested serologically using the Rose Bengal test (RBT) for brucellosis (B. at the melitensis) Croatian Veterinary Institute. In addition, 43 428 samples were tested using the complement fixation test (CFT). The blood samples of sheep and goats were collected from the territories of 20 counties and the City of Zagreb. For bacteriological testing were taken lymph nodes, liver, spleen and
BRUCELLA MELITENSIS IN SMALL RUMINANTS IN THE REPUBLIC OF CROATIA Zeljko Cvetnic1; Silvio Spicic1; Tomislav Kiš 2; Maja Zdelar-Tuk1; Sanja Duvnjak1; Irena Reil1; Boris Habrun1; Miroslav Benic1; Gordan Kompes1 Scientific paper Summary In the period from 2009 to 2013, comprehensive melitococcosis testing was conducted in the Republic of Croatia. During the testing, the Rose Bengal test was applied to 344 019 blood samples of sheep and goats, and positive reactions were confirmed in 1143 (0.3%) of samples. The complement fixation test (confirmatory test) was conducted on 43 428 samples, with positive reactions confirmed in 768 (1.8%) of samples. The organs and tissues of 336 sheep and goats were inspected bacteriologically, and Brucella sp. was isolated in 15 (4.5%) of samples. Bacteriological and molecular techniques (Bru up/Bru-low and Bruce-Ladder) in isolates proved the presence of Brucella melitensis biovar 3. Key words: Brucella melitensis, ruminants, serological and bacteriological tests, Republic of Croatia INTRODUCTION Melitococcosis or brucellosis is a chronic infectious disease of sheep and goats caused by the species Brucella (B.) melitensis. It is one of the most widespread zoonoses worldwide. This paper gives an overview of the distribution of Brucella (B.) melitensis in the Republic of Croatia in the period from 2009 to 2013. Bacteriological and molecular techniques were used to prove and confirm the species Brucella sp. MATERIAL AND METHODS In the investigated period from 2009 to 2013, a total of 344 019 blood samples of sheep and goats were tested serologically using the Rose Bengal test (RBT) for brucellosis (B. melitensis) at the Croatian Veterinary Institute. In addition, 43 428 samples were tested using the complement fixation test (CFT). The blood samples of sheep and goats were collected from the territories of 20 counties and the City of Zagreb. For bacteriological testing were taken lymph nodes, liver, spleen and
781939.B._melitensis_-Sarajevo_-One_Health.docx