2B Trans-presented IL-15 induces pSTAT5 OTII 112010.pzf in T cell immunological synapse. Software used: GraphPad Prism Figure Short title Legend 3A Schematic of experiment to test No data c recruitment of to dendritic cell immunological synapse by IL-15 c 3BCD Image data- contact Recruitment of to dendritic cell michael.dustin@kennedy.ox.ac.uk immunological synapse by IL-15 c 3EFG CgMut_GFP MHCII vs time.xls Recruitment of to dendritic cell Cg-GFP MHC vs time.xls immunological synapse by IL-15 gcMut-GFP MHCII vs time.pzf GFP MHCII vs time.pzf Software used: GraphPad Prism and Microsoft Excel Figure Short title Legend c 4A Images data- contact dependent recruitment of IL-15R to michael.dustin@kennedy.ox.ac.uk sites of MHC engagement c 4BC IL-15Ra DC quant.pzf dependent recruitment of IL-15R to IL-15Ra DC quant dot plots.pzf sites of MHC engagement Software used: GraphPad Prism c 4D dependent IL-15R co-localization il15 mhc pearsons.pzf Software used: GraphPad Prism with MHC class II in model DC immunological synapse Figure Short title Legend 5A IL-15Rα recruitment to the DC Image data- contact immunological synapse michael.dustin@kennedy.ox.ac.uk 5B Analysis of IL-15Rα recruitment to the IL15r rescue.pzf DC immunological synapse Software used: GraphPad Prism Supplementary Figures Figure Short title Legend c S1A Absence of protein in knock out DC Image data- contact siobhan.burns@ucl.ac.uk S1B Absence of IL-21 induced pSTAT5 in Image data- contact c siobhan.burns@ucl.ac.uk -/- DC S1C Surface staining for activation markers DC type: WT, KO (γc-/- DCs)
2B Trans-presented IL-15 induces pSTAT5 in T cell immunological synapse. OTII 112010.pzf Software used: GraphPad Prism Figure Short title Legend 3A Schematic of experiment to test recruitment of c to dendritic cell immunological synapse by IL-15 No data 3BCD Recruitment of c to dendritic cell immunological synapse by IL-15 Image data- contact michael.dustin@kennedy.ox.ac.uk 3EFG Recruitment of c to dendritic cell immunological synapse by IL-15 CgMut_GFP MHCII vs time.xls Cg-GFP MHC vs time.xls gcMut-GFP MHCII vs time.pzf GFP MHCII vs time.pzf Software used: GraphPad Prism and Microsoft Excel Figure Short title Legend 4A c dependent recruitment of IL-15R to sites of MHC engagement Images data- contact michael.dustin@kennedy.ox.ac.uk 4BC c dependent recruitment of IL-15R to sites of MHC engagement IL-15Ra DC quant.pzf IL-15Ra DC quant dot plots.pzf Software used: GraphPad Prism 4D c dependent IL-15R co-localization with MHC class II in model DC immunological synapse il15 mhc pearsons.pzf Software used: GraphPad Prism Figure Short title Legend 5A IL-15Rα recruitment to the DC immunological synapse Image data- contact michael.dustin@kennedy.ox.ac.uk 5B Analysis of IL-15Rα recruitment to the DC immunological synapse IL15r rescue.pzf Software used: GraphPad Prism Supplementary Figures Figure Short title Legend S1A Absence of c protein in knock out DC Image data- contact siobhan.burns@ucl.ac.uk S1B Absence of IL-21 induced pSTAT5 in c -/- DC Image data- contact siobhan.burns@ucl.ac.uk S1C Surface staining for activation markers DC type: WT, KO (γc-/- DCs)
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