SUBMISSION CHECKLIST What is happening What will be the How will the effects How will the effects to the animals? effects? be minimised? be monitored? Anaesthesia Genetic Manipulation Methods Fasting Potential effects Induction drug, dose, route – Maintenance drug, dose, route – Housing Methods of monitoring anaesthesia and recovery Location Additional support during anaesthesia and recovery (eg, Isolation heat, intravenous fluids) Group housing (stocking rates, sexes) Location of induction and recovery areas Shelter Bedding Behaviour Modification Hiding areas Environmental enrichment Stimulus (type, duration, frequency) Duration held Conditioning period Blood/Body Fluid Collection Volume In-vitro studies Route Source of animals Frequency Duration held Anaesthesia or analgesia Euthanasia Restraint Animal monitoring (methods, frequency) Surgery Anaesthesia Diet/Water Modifications Location of pre-operative preparation area Pre-operative preparation Type Surgical procedure (site, technique) Amount Sterile technique (instruments, drapes, surgeon) Effects Location of and housing in post-operative recovery area Measurement of intake Post-operative management Animal monitoring Post-operative monitoring (methods, frequency, duration) Use of analgesics (type, dose, route, frequency, means of Drug Treatments determining necessity for use) Substance Expertise Volume Route frequency/total number per animal Toxicology Local and systemic effects Substance Anaesthesia or analgesia possible side effects Volume Restraint Route frequency of treatments/total number per animal Local and systemic effects Euthanasia Anaesthesia or analgesia Method Restraint Location (where procedure will be performed) Animal monitoring (methods, frequency) Expertise of personnel Endpoint/duration Tumor/neoplasia Wildlife Studies Method Location Site Methods Endpoint Capture methods Animal monitoring (methods, frequency) Handling/restraint Housing Transport Monitoring Type Release Duration Effects on population Confinement Numbers of animals Air-conditioning Teaching Source of animals Housing Duration held Method of disposal
SUBMISSION CHECKLIST What is happening to the animals? What will be the effects? How will the effects be minimised? How will the effects be monitored? Anaesthesia Fasting Induction – drug, dose, route Maintenance – drug, dose, route Methods of monitoring anaesthesia and recovery Additional support during anaesthesia and recovery (eg, heat, intravenous fluids) Location of induction and recovery areas Behaviour Modification Stimulus (type, duration, frequency) Blood/Body Fluid Collection Volume Route Frequency Anaesthesia or analgesia Restraint Animal monitoring (methods, frequency) Diet/Water Modifications Type Amount Effects Measurement of intake Animal monitoring Drug Treatments Substance Volume Route frequency/total number per animal Local and systemic effects Anaesthesia or analgesia possible side effects Restraint Euthanasia Method Location (where procedure will be performed) Expertise of personnel Tumor/neoplasia Method Site Endpoint Animal monitoring (methods, frequency) Transport Type Duration Confinement Numbers of animals Air-conditioning Teaching Source of animals Housing Duration held Method of disposal Genetic Manipulation Methods Potential effects Housing Location Isolation Group housing (stocking rates, sexes) Shelter Bedding Hiding areas Environmental enrichment Duration held Conditioning period In-vitro studies Source of animals Duration held Euthanasia Surgery Anaesthesia Location of pre-operative preparation area Pre-operative preparation Surgical procedure (site, technique) Sterile technique (instruments, drapes, surgeon) Location of and housing in post-operative recovery area Post-operative management Post-operative monitoring (methods, frequency, duration) Use of analgesics (type, dose, route, frequency, means of determining necessity for use) Expertise Toxicology Substance Volume Route frequency of treatments/total number per animal Local and systemic effects Anaesthesia or analgesia Restraint Animal monitoring (methods, frequency) Endpoint/duration Wildlife Studies Location Methods Capture methods Handling/restraint Housing Monitoring Release Effects on population
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