Describe each survival collection of blood in the table below, including any to “pre-bleeds” prior immunizations: Amount of Blood Collected at any one Time Interval(s) Volume time, Number of Between Site of Blood Collection expressed as volume Blood Successive (ml) and as % of Collections Collections (yes/no) body weight (assume 1 ml = 1 gram) Will anesthetics, tranquilizers, or analgesics be administered for blood collection? ( ) No anesthetics, tranquilizers, or analgesics will be administered for blood collection. Explain why ► it is appropriate or necessary NOT to administer pain-relieving agents: ► ( ) Yes. Describe the administration of pain-relieving agents, including the name of each agent, and ► its dose (mg/kg), volume (ml), and route and frequency/duration of administration (Make sure this information is also included in Appendix 3): ► Will volume replacement be provided for blood that is collected? ( ) Volume will NOT be replaced for some of the blood collection listed. For each collection listed in ► Item 2.a, above, for which volume will NOT be replaced, explain why not. ► ( ) Volume WILL be replaced for some of the blood collection listed. For each collection listed in ► Item 2.a, above, for which volume WILL be replaced, describe the replacement(s) that will be provided (including the composition of the replacement(s), volume, and route of administration). ► Terminal Blood Collection. Will animals be euthanatized by exsanguination, for harvest of antibodies? ( ) No, this protocol does NOT involve terminal blood collection for harvest of antibodies. ► ( ) Yes, this protocol DOES require terminal blood collection for the harvest of antibodies. Make sure ► this is included in Item R of the ACORP, and complete Items 3.a., 3. b., and 3.c., below: Describe the method(s) to be used for euthanasia and exsanguination: ► Will anesthetics, tranquilizers, or analgesics be administered for exsanguination? ( ) No anesthetics, tranquilizers, or analgesics will be administered for the exsanguination(s). ► Explain why it is appropriate or necessary NOT to administer pain-relieving agents:
Describe each survival collection of blood in the table below, including any “pre-bleeds” prior to immunizations: Site of Blood Collection Amount of Blood Collected at any one time, expressed as volume (ml) and as % of body weight (assume 1 ml = 1 gram) Number of Blood Collections Time Interval(s) Between Successive Collections Volume (yes/no) Will anesthetics, tranquilizers, or analgesics be administered for blood collection? ► ( ) No anesthetics, tranquilizers, or analgesics will be administered for blood collection. Explain why it is appropriate or necessary NOT to administer pain-relieving agents: ► ► ( ) Yes. Describe the administration of pain-relieving agents, including the name of each agent, and its dose (mg/kg), volume (ml), and route and frequency/duration of administration (Make sure this information is also included in Appendix 3): ► Will volume replacement be provided for blood that is collected? ► ( ) Volume will NOT be replaced for some of the blood collection listed. For each collection listed in Item 2.a, above, for which volume will NOT be replaced, explain why not. ► ► ( ) Volume WILL be replaced for some of the blood collection listed. For each collection listed in Item 2.a, above, for which volume WILL be replaced, describe the replacement(s) that will be provided (including the composition of the replacement(s), volume, and route of administration). ► Terminal Blood Collection. Will animals be euthanatized by exsanguination, for harvest of antibodies? ► ( ) No, this protocol does NOT involve terminal blood collection for harvest of antibodies. ► ( ) Yes, this protocol DOES require terminal blood collection for the harvest of antibodies. Make sure this is included in Item R of the ACORP, and complete Items 3.a., 3. b., and 3.c., below: Describe the method(s) to be used for euthanasia and exsanguination: ► Will anesthetics, tranquilizers, or analgesics be administered for exsanguination? ► ( ) No anesthetics, tranquilizers, or analgesics will be administered for the exsanguination(s). Explain why it is appropriate or necessary NOT to administer pain-relieving agents:
ACORP_Appendix_2.docx