Phytoplankton, pigments, picoplankton, Suspended solids Water • – “Integrated Column” sample: Measure and Record the residual volume left after the chemistry samplings • in the surface niskin bottle, because this is the only Carbon sampling depth common to all sites. Base the collection of the remaining residual volumes, to be added to the 20 • litre carboy sample, on the measured residual volume from the “integrated” surface niskin bottle to avoid a volume bias from a particular depth. Use the measuring jugs and funnels made from plastic for this “native/natural“ Discard from niskins sampled deeper than the surface bottle, anything • in excess of the volume equal to that obtained from the full sampling conducted from the surface niskin. Do not include water sampled from deeper than 50m in the carboy. • Phytoplankton pigments samples from depths equating to moored WQM sensor – • packs: There is a requirement for another cast where a niskin of water is collected • from each of the 2 depths that correlate with the moored WQM sensors, for pigment analysis (by HPLC) to check WQM pigment performance As with the water collected for the carboy sample, use the • “Water Column” funnels made from plastic to collect the niskin contents into “native/natural“ individual, labelled, 5 litre containers. Genomic surface sampling for microbial and phytoplankton analyses requires a 3 litre niskin sample collection from the same depths Remember to keep all collected waters cold/cool and in the shade to minimise • sample degradation. Keep collected waters in carboys and containers quite separate from other samples • which may hold any formalin whatsoever Where and when necessary, preserve or pre-treat samples as outlined in the • detailed sampling protocols for each parameter as described below Endeavour to avoid sample biodegradation by holding these samples from • collection time to post-processing, to less than four (4) hours maximum. IMOS National Reference Station Field Sampling BIOGEOCHEMICAL OPERATIONS MANUAL For discrete water samples to be taken, 5L Niskin bottles triggered by messenger (Brass
• Phytoplankton, pigments, picoplankton, Suspended solids – “Integrated Water Column” sample: • Measure and Record the residual volume left after the chemistry samplings in the surface niskin bottle, because this is the only Carbon sampling depth common to all sites. • Base the collection of the remaining residual volumes, to be added to the 20 litre “integrated” carboy sample, on the measured residual volume from the surface niskin bottle to avoid a volume bias from a particular depth. Use the measuring jugs and funnels made from “native/natural“ plastic for this • Discard from niskins sampled deeper than the surface bottle, anything in excess of the volume equal to that obtained from the full sampling conducted from the surface niskin. • Do not include water sampled from deeper than 50m in the carboy. • Phytoplankton pigments – samples from depths equating to moored WQM sensor packs: • There is a requirement for another cast where a niskin of water is collected from each of the 2 depths that correlate with the moored WQM sensors, for pigment analysis (by HPLC) to check WQM pigment performance • As with the water collected for the “Water Column” carboy sample, use the funnels made from “native/natural“ plastic to collect the niskin contents into individual, labelled, 5 litre containers. Genomic surface sampling for microbial and phytoplankton analyses requires a 3 litre niskin sample collection from the same depths • Remember to keep all collected waters cold/cool and in the shade to minimise sample degradation. • Keep collected waters in carboys and containers quite separate from other samples which may hold any formalin whatsoever • Where and when necessary, preserve or pre-treat samples as outlined in the detailed sampling protocols for each parameter as described below • Endeavour to avoid sample biodegradation by holding these samples from collection time to post-processing, to less than four (4) hours maximum. IMOS National Reference Station Field Sampling BIOGEOCHEMICAL OPERATIONS MANUAL For discrete water samples to be taken, 5L Niskin bottles triggered by messenger (Brass
AA_NRS_BGC_Handbook_ver2.2.docx