Patch Test Methodology for Latex Glove Allergy Individuals who have skin complaints should be assessed with patch testing. The standard protocol used by the North American Contact Dermatitis Group (NACDG) should be employed as described below. The allergens to be routinely tested include: Black rubber mix 1% Carba mix 3% Ethylenediamine 2HCL 1% Imidazolodinyl urea 2% Mercaptobenzothiazole 1% Mercapto mix 1% O-Phenylenediamine 1% Thiuram mix 1% Triclosan 2% Volunteer-supplied latex glove If the worker is exposed to glutaraldehyde (Cidex, Sporocidin) in the course of their work, this should also be included. METHOD: The allergens are applied to Finn chambers. The Finn chambers are placed on the upper back and affixed with Scanpor tape. The patches are left on for 48 hours. The patches are then removed and the sites left for 15 to 30 minutes to let the pressure effects wear off. The first reading is then completed. At 96 hours, the second reading is performed. The sites are scored as follows: 0: Negative or "doubtful" reaction 1: Weak (non-vesicular) reaction 2: Strong (edematous or vesicular) reaction 3: Extreme (bulbous or ulcerative) reaction 1R: Irritant reaction
Patch Test Methodology for Latex Glove Allergy Individuals who have skin complaints should be assessed with patch testing. The standard protocol used by the North American Contact Dermatitis Group (NACDG) should be employed as described below. The allergens to be routinely tested include: Black rubber mix 1% Carba mix 3% Ethylenediamine 2HCL 1% Imidazolodinyl urea 2% Mercaptobenzothiazole 1% Mercapto mix 1% O-Phenylenediamine 1% Thiuram mix 1% Triclosan 2% Volunteer-supplied latex glove If the worker is exposed to glutaraldehyde (Cidex, Sporocidin) in the course of their work, this should also be included. METHOD: The allergens are applied to Finn chambers. The Finn chambers are placed on the upper back and affixed with Scanpor tape. The patches are left on for 48 hours. The patches are then removed and the sites left for 15 to 30 minutes to let the pressure effects wear off. The first reading is then completed. At 96 hours, the second reading is performed. The sites are scored as follows: 0: Negative or "doubtful" reaction 1: Weak (non-vesicular) reaction 2: Strong (edematous or vesicular) reaction 3: Extreme (bulbous or ulcerative) reaction 1R: Irritant reaction
ACAAI%20Patch%20Test%20Methodology%20for%20Latex%20Glove%20Allergy.docx