If the measurement is delayed the next day, specimens should be stored at 5 ± 4 °C. If the analytical system fails, specimens and any partially-prepared / extracted samples can be stored at 5 ± 4 °C until the analytical system is again operational. Studies indicate THC is very stable when stored at varying temperatures, during freeze/thaw cycles, and in different matrices, which allows for repeat analysis. 3.5 Special equipment Blood specimens are collected from subjects in standard blood collection tubes. 4.0 Reagents or Media 4.1 Reagents and sources Reagents and sources used during the development and validation of this method are listed in Table 2. Specific part numbers and manufacturers are listed only as examples and other sources can be used for purchasing these reagents as long as the quality meets or exceeds the established standards listed in this SOP. All chemicals and solvents are used without further purification. All solid state reagents/standards are acceptable for use for 10 years from the date of open when stored as recommended by the manufacturer unless otherwise indicated through stability studies or by the manufacturer. All liquid state reagents and standards are acceptable for use for 1 year from the date of open when stored as recommended by the manufacturer unless otherwise indicated through stability studies or by the manufacturer. Table 2. Chemicals and sources Part Reagent Grade Source * Number Fisher Scientific, Formic Acid Optima-LC/MS A117-50 Fairlawn, NJ Deionized 18 MΩ Type 1 Water Millipore n/a water Fisher Scientific, Ammonium Formate Optima-LC/MS A115-50 Fairlawn, NJ Fisher Scientific, Methanol Optima-LC/MS A456-4 Fairlawn, NJ Methyl tert-butyl ether Acros Organics, 99.9% 378720025 (MTBE) Fairlawn, NJ Fisher Scientific, Hexanes Optima H303-4 Fairlawn, NJ Fisher Scientific, Acetonitrile Optima-LC/MS A955-4 Fairlawn, NJ * Or equivalent. 4.2 Reagent Preparation
If the measurement is delayed the next day, specimens should be stored at 5 ± 4 °C. If the analytical system fails, specimens and any partially-prepared / extracted samples can be stored at 5 ± 4 °C until the analytical system is again operational. Studies indicate THC is very stable when stored at varying temperatures, during freeze/thaw cycles, and in different matrices, which allows for repeat analysis. 3.5 Special equipment Blood specimens are collected from subjects in standard blood collection tubes. 4.0 Reagents or Media 4.1 Reagents and sources Reagents and sources used during the development and validation of this method are listed in Table 2. Specific part numbers and manufacturers are listed only as examples and other sources can be used for purchasing these reagents as long as the quality meets or exceeds the established standards listed in this SOP. All chemicals and solvents are used without further purification. All solid state reagents/standards are acceptable for use for 10 years from the date of open when stored as recommended by the manufacturer unless otherwise indicated through stability studies or by the manufacturer. All liquid state reagents and standards are acceptable for use for 1 year from the date of open when stored as recommended by the manufacturer unless otherwise indicated through stability studies or by the manufacturer. Table 2. Chemicals and sources Reagent Grade Source * Part Number Formic Acid Optima-LC/MS Fisher Scientific, Fairlawn, NJ A117-50 Type 1 Water Deionized 18 MΩ water Millipore n/a Ammonium Formate Optima-LC/MS Fisher Scientific, Fairlawn, NJ A115-50 Methanol Optima-LC/MS Fisher Scientific, Fairlawn, NJ A456-4 Methyl tert-butyl ether (MTBE) 99.9% Acros Organics, Fairlawn, NJ 378720025 Hexanes Optima Fisher Scientific, Fairlawn, NJ H303-4 Acetonitrile Optima-LC/MS Fisher Scientific, Fairlawn, NJ A955-4 * Or equivalent. 4.2 Reagent Preparation
AgilentTHCBloodSOP.docx