At the time of preparation, all reagents should be labeled with name (or initials) of person preparing the solution, expiration date, and contents of solution. Reagents are prepared as needed. Records of all preparations are kept in the Reagent Preparation Spreadsheet and are noted in the laboratory notebook. Instructions listed in the following sections (4.2.1 – 4.2.5) are provided as example calculations and care should be taken to ensure final concentrations remain consistent. For example, stock concentrations of purchased materials may vary between vendors, and therefore, dilutions and final protocols have to be adjusted accordingly. All prepared reagents are stored at room temperature unless otherwise indicated. HPLC mobile phases 100% Water with 0.1% Formic Acid (Mobile Phase A): Spike 1 mL of formic acid into 1000 mL type-1 water. This solution is good for 1 month. 100% Acetonitrile with 0.1% Formic Acid (Mobile Phase B): Spike 1 mL of formic acid into 1000 mL acetonitrile. This solution is good for 1 month. 0.1% Aqueous Formic Acid Add 1000uL formic acid to 1000 mL type 1 water. This solution is good for 1 month. Needle Rinse Solution 75% MeOH – Add 750 mL MeOH to 250 mL type 1 This solution is good for 1 month. Native Standards The standards are pre-laced in wells of a 48-well plate and purchased from Cayman Chemical. The standards are stored at - ≤ 20 °C. Other equivalent standards may be purchased from another vendor, if necessary, and the plates can be made by hand. Isotopically labeled internal standards The labeled internal standards are pre-laced in wells of a 48-well plate and purchased from Cayman Chemical. The plates are stored at -20 °C. Other equivalent standards may be purchased from ≤ another vendor, if necessary, and the plates can be made by hand. QC Material QC Material are pre-laced and dried in wells of a 48-well plate and purchased from Cayman Chemical. The plates are stored at -20 ≤ °C. Other equivalent standards may be purchased from another vendor, if necessary, and the plates can be made by hand. 4.3 Preparation of Standards
At the time of preparation, all reagents should be labeled with name (or initials) of person preparing the solution, expiration date, and contents of solution. Reagents are prepared as needed. Records of all preparations are kept in the Reagent Preparation Spreadsheet and are noted in the laboratory notebook. Instructions listed in the following sections (4.2.1 – 4.2.5) are provided as example calculations and care should be taken to ensure final concentrations remain consistent. For example, stock concentrations of purchased materials may vary between vendors, and therefore, dilutions and final protocols have to be adjusted accordingly. All prepared reagents are stored at room temperature unless otherwise indicated. HPLC mobile phases 100% Water with 0.1% Formic Acid (Mobile Phase A): Spike 1 mL of formic acid into 1000 mL type-1 water. This solution is good for 1 month. 100% Acetonitrile with 0.1% Formic Acid (Mobile Phase B): Spike 1 mL of formic acid into 1000 mL acetonitrile. This solution is good for 1 month. 0.1% Aqueous Formic Acid Add 1000uL formic acid to 1000 mL type 1 water. This solution is good for 1 month. Needle Rinse Solution – 75% MeOH Add 750 mL MeOH to 250 mL type 1 This solution is good for 1 month. Native Standards The standards are pre-laced in wells of a 48-well plate and purchased from Cayman Chemical. The standards are stored at ≤ °C. Other equivalent standards may be purchased from another vendor, if necessary, and the plates can be made by hand. Isotopically labeled internal standards The labeled internal standards are pre-laced in wells of a 48-well plate and purchased from Cayman Chemical. The plates are stored at ≤ -20 °C. Other equivalent standards may be purchased from another vendor, if necessary, and the plates can be made by hand. QC Material QC Material are pre-laced and dried in wells of a 48-well plate and purchased from Cayman Chemical. The plates are stored at ≤ -20 °C. Other equivalent standards may be purchased from another vendor, if necessary, and the plates can be made by hand. 4.3 Preparation of Standards 20 -
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