Origin of replication for pBR322 14,671 14,951 281 pBR322 Bacterial origin of replication1 – (pBR322 ori) Intervening sequence 14,952 15,241 290 pBR322 pCambia-1301 backbone1 – Neomycin phosphotransferase II (nptII) Aminoglycoside phosphotransferase for kanamycin resistance; to selective 15,242 16,036 795 Tn5 transposon – gene positive bacterial transformants1 Intervening sequence 16,037 16,231 195 Vector DNA pCambia-1301backbone1 – Terminator of the ubiquitin-3 gene 16,232 16,586 355 S. tuberosum Terminator for ipt gene transcription – (tUbi3) A. tumefaciens Intervening sequence 16,587 16,937 351 Sequence for DNA cloning – Ti-plasmid A. tumefaciens Isopentenyl transferase (ipt) gene 16,938 17,660 723 Allows for identification of plantlets that have incorporated the backbone DNA – Ti-plasmid Intervening sequence 17,661 17,672 12 Synthetic DNA Sequence used for DNA cloning – S. tuberosum Polyubiquitin promoter (Ubi7) 17,673 19,410 1,738 Promoter to drive expression of the ipt backbone marker gene – (var. Ranger Russet) Intervening sequence 19,411 19,660 250 Vector DNA pCambia-1301 backbone1 – 1pCAMBIA-1301 sequence as indicated by the blue shading is available @
Origin of replication for pBR322 (pBR322 ori) 14,671 – 14,951 281 pBR322 Bacterial origin of replication1 Intervening sequence 14,952 – 15,241 290 pBR322 pCambia-1301 backbone1 Neomycin phosphotransferase II (nptII) gene 15,242 – 16,036 795 Tn5 transposon Aminoglycoside phosphotransferase for kanamycin resistance; to selective positive bacterial transformants1 Intervening sequence 16,037 – 16,231 195 Vector DNA pCambia-1301backbone1 Terminator of the ubiquitin-3 gene (tUbi3) 16,232 – 16,586 355 S. tuberosum Terminator for ipt gene transcription Intervening sequence 16,587 – 16,937 351 A. tumefaciens Ti-plasmid Sequence for DNA cloning Isopentenyl transferase (ipt) gene 16,938 – 17,660 723 A. tumefaciens Ti-plasmid Allows for identification of plantlets that have incorporated the backbone DNA Intervening sequence 17,661 – 17,672 12 Synthetic DNA Sequence used for DNA cloning Polyubiquitin promoter (Ubi7) 17,673 – 19,410 1,738 S. tuberosum (var. Ranger Russet) Promoter to drive expression of the ipt backbone marker gene Intervening sequence 19,411 – 19,660 250 Vector DNA pCambia-1301 backbone1 1pCAMBIA-1301 sequence as indicated by the blue shading is available @
A1139_SD%20at%20Approval.docx