STANDARD OPERATING PROCEDURE (SOP) Agilent 1200 HPLC System for Sugar Analysis Purchased May 2010 Location: 2115, Pulp and Paper Labs Contact: Dr. Sunkyu Park (), 919-515-0473 To schedule training contact: Hui Chen (), 919-809-4751 Technical service: 1-800-227-9770 Sales Rep.: Tom Breeden (919) 466-2032 1. Preparation Equipment: the following colors are used to indicate the status of the module or instrument: Color Status Red Error Yellow Not ready Purple Pre-run, post-run Blue Run Light blue Injecting Green Idle Dark gray Offline Light gray Standby (for example, lamps off) Calibration standards: We are making up stock solutions of the following sugars: Glucose, xylose, galactose, and arabinose with mannose together An excel program (Linda’s HPLC standard sugar solution makeup) has the makeup and calculates the amounts needed to make up 6 calibration standards. The concentrations are kept constant. Place the 2 ml vials in the holder as follows: slot (1) is always Milli-Q water, followed by the standards in slots (2) (7). – Instrument 1 online: Turn on the five sections of the equipment, click on Instrument 1 online to set up for analysis.
STANDARD OPERATING PROCEDURE (SOP) Agilent 1200 HPLC System for Sugar Analysis Purchased May 2010 Location: 2115, Pulp and Paper Labs Contact: Dr. Sunkyu Park (), 919-515-0473 To schedule training contact: Hui Chen (), 919-809-4751 Technical service: 1-800-227-9770 Sales Rep.: Tom Breeden (919) 466-2032 1. Preparation Equipment: the following colors are used to indicate the status of the module or instrument: Color Status Red Error Yellow Not ready Purple Pre-run, post-run Blue Run Light blue Injecting Green Idle Dark gray Offline Light gray Standby (for example, lamps off) Calibration standards: We are making up stock solutions of the following sugars: Glucose, xylose, galactose, and arabinose with mannose together An excel program (Linda’s HPLC standard sugar solution makeup) has the makeup and calculates the amounts needed to make up 6 calibration standards. The concentrations are kept constant. Place the 2 ml vials in the holder as follows: slot (1) is always Milli-Q water, followed by the standards in slots (2) – (7). Instrument 1 online: Turn on the five sections of the equipment, click on Instrument 1 online to set up for analysis.
AgilentHPLCSOP-1.docx