Peptone 20g Yeast extract 2g Glycerol 15ml K2HPO4 1 g MgSO4 1 g Agar 3g Weigh and add to Schott bottle and make up to 1000 ml using deionised water. The pH should be 7.0. Autoclave at 121 °C for 15 minutes Then add: Streptomycin sulphate 500 µg/ml Cycloheximide 50 µg/ml Thallium acetate 50 µg/ml
Peptone Yeast extract Glycerol K2HPO4 MgSO4 Agar 20g 2g 15ml 1 g 1 g 3g Weigh and add to Schott bottle and make up to 1000 ml using deionised water. The pH should be 7.0. Autoclave at 121 °C for 15 minutes Then add: Streptomycin sulphate Cycloheximide Thallium acetate 500 µg/ml 50 µg/ml 50 µg/ml
aerobic_chapter_10.docx