time t: scanning speed of laser beam v: radius in the focal plane of a circular bleached region w: Gaussian axial width at the level of the photobleaching beam zb: e-2 Gaussian axial width at the level of the imaging PSF zi: e-2 distance between two scanning y: : photobleaching parameter; also used in some equations as diffusion anomaly coefficient  : typical length scales in the microstructure standard deviation : : dynamic viscosity  transport coefficient, time-dependent diffusion : wavelength : chemical potential : 1. Introduction 1.1 Principle of FRAP Fluorescence recovery after photobleaching (FRAP) is a widely established and powerful fluorescence microscopy based method for obtaining information on the dynamics of mobile fluorescent molecules at the micrometer scale. Specifically, FRAP can be used to obtain quantitative information on the micrometer scale of the diffusion process, transient binding events and directed transport.
t: time v: scanning speed of laser beam w: radius in the focal plane of a circular bleached region zb: Gaussian axial width at the e-2 level of the photobleaching beam zi: Gaussian axial width at the e-2 level of the imaging PSF y: distance between two scanning  : photobleaching parameter; also used in some equations as diffusion anomaly coefficient : typical length scales in the microstructure : standard deviation  : dynamic viscosity : transport coefficient, time-dependent diffusion : wavelength : chemical potential 1. Introduction 1.1 Principle of FRAP Fluorescence recovery after photobleaching (FRAP) is a widely established and powerful fluorescence microscopy based method for obtaining information on the dynamics of mobile fluorescent molecules at the micrometer scale. Specifically, FRAP can be used to obtain quantitative information on the micrometer scale of the diffusion process, transient binding events and directed transport.
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