on the solubility in different solvents, as shown in Table 5 (Kwon et al., 1996; Mepba & Achinewhu, 2003; Sant’Anna, Freitas, & Coelho, 2003) or apply enzymes for simultaneous recovery of oil and protein (Mepba & Achinewhu, 2003; Samson, Khaund, Cater, & Mattil, 1971; Sant’Anna et al., 2003). Table 5. Protein fractionation based on the modified method of Hu and Essen (Kwon et al., 1996). Total protein Protein fraction Extraction solvent extracted (%) Albumins Deionised water 21.0 (0.4)a Globulins 0.5M NaCl 40.1 (0.3) Prolamines 70% isopropyl alcohol 3.3 (0.2) Glutelins-1 50% Acetic acid 14.4 (0.4) Glutelins-2 0.1M NaOH 4.8 (0.7) Unextractable protein 8.1 (0.9) a Values in parenthesis are standard deviations obtained from triplicates (Kwon et al., 1996) The wet processing method for protein extraction developed here was designed to be simple and to expand the diversity of coconut products, recovering not only the protein but also the oil and skim coconut milk on a fresh basis and under controlled processing conditions.
on the solubility in different solvents, as shown in Table 5 (Kwon et al., 1996; Mepba & Achinewhu, 2003; Sant’Anna, Freitas, & Coelho, 2003) or apply enzymes for simultaneous recovery of oil and protein (Mepba & Achinewhu, 2003; Samson, Khaund, Cater, & Mattil, 1971; Sant’Anna et al., 2003). Table 5. Protein fractionation based on the modified method of Hu and Essen (Kwon et al., 1996). Protein fraction Extraction solvent Total protein extracted (%) Albumins Globulins Prolamines Glutelins-1 Glutelins-2 Unextractable protein Deionised water 0.5M NaCl 70% isopropyl alcohol 50% Acetic acid 0.1M NaOH 21.0 (0.4)a 40.1 (0.3) 3.3 (0.2) 14.4 (0.4) 4.8 (0.7) 8.1 (0.9) a Values in parenthesis are standard deviations obtained from triplicates (Kwon et al., 1996) The wet processing method for protein extraction developed here was designed to be simple and to expand the diversity of coconut products, recovering not only the protein but also the oil and skim coconut milk on a fresh basis and under controlled processing conditions.
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