Equipment (per group) Basic food test iodine solution in dropping bottle spotting tile. biuret reagent in dropping bottle boiling tubes/test tubes (three per food sample) reagent in dropping bottle Benedict’s Beaker heating device (e.g. kettle/thermostatic water bath or Bunsen burner). Sugar paper ethanol in a dropping bottle wash bottle filled with water bung. DNA extraction one leek 1 teaspoon salt tea spoon washing-up liquid pre-chilled alcohol (pre-chilled overnight to -20ºC then kept on ice) pestle mortar boiling tube pineapple juice inoculation loop heating device (e.g. kettle/thermostatic water bath or Bunsen burner). Health and Safety At some concentrations iodine solutions can be harmful look at any labelling on the dropper – bottle. Biuret solution contains NaOH. Low concentrations NaOH it is an irritant but at higher concentrations it is corrosive look at any labelling on the dropper bottle. – Ethanol is highly flammable. Do not use this near a naked flame (e.g. Bunsen burner). If you are allergic to any foods let your teacher know before starting this practical.
Equipment (per group) Basic food test iodine solution in dropping bottle spotting tile. biuret reagent in dropping bottle boiling tubes/test tubes (three per food sample) Benedict’s reagent in dropping bottle Beaker heating device (e.g. kettle/thermostatic water bath or Bunsen burner). Sugar paper ethanol in a dropping bottle wash bottle filled with water bung. DNA extraction one leek 1 teaspoon salt tea spoon washing-up liquid pre-chilled alcohol (pre-chilled overnight to -20ºC then kept on ice) pestle mortar boiling tube pineapple juice inoculation loop heating device (e.g. kettle/thermostatic water bath or Bunsen burner). Health and Safety At some concentrations iodine solutions can be harmful – look at any labelling on the dropper bottle. Biuret solution contains NaOH. Low concentrations NaOH it is an irritant but at higher concentrations it is corrosive – look at any labelling on the dropper bottle. Ethanol is highly flammable. Do not use this near a naked flame (e.g. Bunsen burner). If you are allergic to any foods let your teacher know before starting this practical.
310656-pag-activity-biology-testing-for-biological-molecules-suggestion-1.docx