Supplementary Table 2 PCR primer sequences and conditions of molecular markers used in this study and for cloning full-length coding sequences of and BTC1 into vectors for Yeast-2-Hybrid experiments. BvBBX19 Fragment Fragment size [bp] Marker Target Primer Fragment size [bp] Target Marker type Primer sequence 5`-->3` PCR conditions Pollinator name vector name size [bp] Seed parent (056822/15) 94°C, 2' + [(94°, 30'' + A882a GTGACTCATTATCTTGGACAG CAU4234 InDel 60°C, 30'' + 72°C, 45'') x 790 1100 BTC1 A883 AGAAAGTGATGACTCAACTCG 32] + 72°C, 5' CAPS, 94°C, 2' + [(94°, 30'' + N0150_F ATGCATCTTATACTATGGGACCAC CAU 4235 digestion 57°C, 30'' + 72°C, 60'') x 742 537 + 205 BvBBX19 N0150_R GGAACAATGCTTGCCGAATCAC with 32] + 72°C, 5' DraIII 98°C, 30'' + [(98°, 10'' + - pACT2 71°C, 30'' + 72°C, 1') x 2384 43] + 72°C, 2' - BTC1 98°C, 30'' + [(98°, 10'' + - pAS2-1 70°C, 30'' + 72°C, 1') x 2384 43] + 72°C, 2' 605 98°C, 30'' + [(98°, 10'' + - pACT2 71°C, 30'' + 72°C, 1') x 695 43] + 72°C, 2' - BvBBX19 605 98°C, 30'' + [(98°, 10'' + - pAS2-1 72°C, 30'' + 72°C, 1') x 695 43] + 72°C, 2'
Supplementary Table 2 PCR primer sequences and conditions of molecular markers used in this study and for cloning full-length coding sequences of BTC1 and BvBBX19 into vectors for Yeast-2-Hybrid experiments. Marker name Target Marker type Target vector Primer name Primer sequence 5`-->3` PCR conditions Fragment size [bp] Fragment size [bp] Seed parent (056822/15) Fragment size [bp] Pollinator CAU4234 BTC1 InDel A882a GTGACTCATTATCTTGGACAG 94°C, 2' + [(94°, 30'' + 60°C, 30'' + 72°C, 45'') x 32] + 72°C, 5' 790 1100 A883 AGAAAGTGATGACTCAACTCG CAU 4235 BvBBX19 CAPS, digestion with DraIII N0150_F ATGCATCTTATACTATGGGACCAC 94°C, 2' + [(94°, 30'' + 57°C, 30'' + 72°C, 60'') x 32] + 72°C, 5' 742 537 + 205 N0150_R GGAACAATGCTTGCCGAATCAC - BTC1 - pACT2 98°C, 30'' + [(98°, 10'' + 71°C, 30'' + 72°C, 1') x 43] + 72°C, 2' 2384 - pAS2-1 98°C, 30'' + [(98°, 10'' + 70°C, 30'' + 72°C, 1') x 43] + 72°C, 2' 2384 - BvBBX19 - pACT2 98°C, 30'' + [(98°, 10'' + 71°C, 30'' + 72°C, 1') x 43] + 72°C, 2' 605 695 - pAS2-1 98°C, 30'' + [(98°, 10'' + 72°C, 30'' + 72°C, 1') x 43] + 72°C, 2' 605 695
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