Electronic Supplementary Material Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer Sang-Choon Lee1 · Min-Seo Kim1 · Ki-Chun Yoo2 · Na-Reum Ha1 · Su-Jae Lee2 · Moon-Young Yoon1 1Department of Chemistry and 2Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea List of Figures and Tables Biopanning for peptides. Fig. S1 Salmonella-binding Fig. S2 Phage Binding Affinity and Specificity. Fig. S3 The Chemical Structure of Synthesized Binding Peptides. Salmonella Peptide Binding Affinity and Specificity. Fig. S4 Fig. S5 Binding Affinity of PDPPs. Fig. S6 Binding Specificity test of PDPPs. Fig. S7 Feasibility test for PDPPs in tap water Fig. S8 PDPP binding efficiency via visualization of and S. enteritidis S. typhimurium. Fig. S9 Comparison of the Binding Efficiency of PDPPs and sc-PDPP against Salmonella. Fig. S10 Specificity of SEB-PDPP 1 between and S. enteritidis V. parahaemolyticus. Fig. S11 Specificity of STB-PDPP 1 and SRB-PDPP 2 between and S. typhimurium V. parahaemolyticus. Fig. S12 Estimation of the Limit of Detection for PDPPs. Table S1 Conditions for each Round of Biopanning. Table S2 and Binding Peptides Displayed on the M13 Phage. S. enteritidis S. typhimurium Materials and Methods Estimation of Binding Affinities and Specificities for Screened Phage via ELISA. The individual S. binding phage was prepared as described above. The amplified phages were enteritidis and S. typhimurium titered to determine the phage concentration, plaque forming unit (PFU), by counting the number of plaques. To estimate the binding affinities, the cells (108 CFU mL-1) with a negatively charged membrane surface were
Electronic Supplementary Material Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer Sang-Choon Lee1 · Min-Seo Kim1 · Ki-Chun Yoo2 · Na-Reum Ha1 · Su-Jae Lee2 · Moon-Young Yoon1 1Department of Chemistry and 2Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea List of Figures and Tables Fig. S1 Biopanning for Salmonella-binding peptides. Fig. S2 Phage Binding Affinity and Specificity. Fig. S3 The Chemical Structure of Synthesized Salmonella Binding Peptides. Fig. S4 Peptide Binding Affinity and Specificity. Fig. S5 Binding Affinity of PDPPs. Fig. S6 Binding Specificity test of PDPPs. Fig. S7 Feasibility test for PDPPs in tap water Fig. S8 PDPP binding efficiency via visualization of S. enteritidis and S. typhimurium. Fig. S9 Comparison of the Binding Efficiency of PDPPs and sc-PDPP against Salmonella. Fig. S10 Specificity of SEB-PDPP 1 between S. enteritidis and V. parahaemolyticus. Fig. S11 Specificity of STB-PDPP 1 and SRB-PDPP 2 between S. typhimurium and V. parahaemolyticus. Fig. S12 Estimation of the Limit of Detection for PDPPs. Table S1 Conditions for each Round of Biopanning. Table S2 S. enteritidis and S. typhimurium Binding Peptides Displayed on the M13 Phage. Materials and Methods Estimation of Binding Affinities and Specificities for Screened Phage via ELISA. The individual S. enteritidis and S. typhimurium binding phage was prepared as described above. The amplified phages were titered to determine the phage concentration, plaque forming unit (PFU), by counting the number of plaques. To estimate the binding affinities, the cells (108 CFU mL-1) with a negatively charged membrane surface were
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