demonstrates the limited diffusion through a 25µm thick layer of PHBV to limit cells growth. This may result in a dryer apical surface at air-liquid as the water film forms at the PHBV layer. Bilayers of Alvetex®-PHBV and PLLA-PHBV Figure 789: MTT cell viability for Bilayers of Alvetex®-PHBV and PLLA-PHBV. Bilayer of Alvetex-PHBV n=1; Bilayer of PLLA-PHBV fibroblast monoculture n=1; Bilayer of PLLA-PHBV coculture 0mM SDS n=2 and 8mM SDS n=3. The error bars represent the standard deviation between experimental repeats. Alvetex®-PHBV co-cultures were identical for 2mL of PHBV but viability was slightly higher for 4mL PHBV in 5mL of medium. (n=1 PLLA-PHBV co-cultures were significantly higher for 1x106 fibroblasts over 5x105 fibroblasts.(n=1) PLLA-PHBV fibroblast monoculture had little higher 1x106 fibroblasts over 5x105 fibroblasts.(n=2) This demonstrated that a lower seeding density of fibroblast catches up with the higher or that there is a cell population limit. PLLA-PHBV co-cultures were significantly higher for 1x106 fibroblasts over 5x105 fibroblasts when irritated using the 42bis protocol with 8mM SDS. (n=3)
demonstrates the limited diffusion through a 25µm thick layer of PHBV to limit cells growth. This may result in a dryer apical surface at air-liquid as the water film forms at the PHBV layer. Bilayers of Alvetex®-PHBV and PLLA-PHBV Figure 789: MTT cell viability for Bilayers of Alvetex®-PHBV and PLLA-PHBV. Bilayer of Alvetex-PHBV n=1; Bilayer of PLLA-PHBV fibroblast monoculture n=1; Bilayer of PLLA-PHBV coculture 0mM SDS n=2 and 8mM SDS n=3. The error bars represent the standard deviation between experimental repeats. Alvetex®-PHBV co-cultures were identical for 2mL of PHBV but viability was slightly higher for 4mL PHBV in 5mL of medium. (n=1 PLLA-PHBV co-cultures were significantly higher for 1x106 fibroblasts over 5x105 fibroblasts.(n=1) PLLA-PHBV fibroblast monoculture had little higher 1x106 fibroblasts over 5x105 fibroblasts.(n=2) This demonstrated that a lower seeding density of fibroblast catches up with the higher or that there is a cell population limit. PLLA-PHBV co-cultures were significantly higher for 1x106 fibroblasts over 5x105 fibroblasts when irritated using the 42bis protocol with 8mM SDS. (n=3)
3D%20RECONSTRUCTED%20SKIN%20EQUIVALENT%20MODELS%20FOR%20IRRITANT%20TESTING.docx