Electrophoresis Standard Operating Procedure Revision Date: 09/14/18 Applies To: Employees responsible for writing lab-specific standard operating procedures; and all faculty, staff, students, and other employees working with electrophoresis. This standard operating procedure (SOP) outlines the process of electrophoresis, including the use of equipment and chemicals necessary for the process. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when performing electrophoresis. [Provide additional information as it pertains to your research protocol] Electrophoresis uses electrical energy to separate molecules based on their size, structure, and electrical charge. [Write the steps for using the chemical in your research protocol] [Provide additional information as it pertains to your research protocol] Electrophoresis equipment can pose significant electrical hazard in the laboratory. Typical electrophoresis units operating at 100 volts can provide a lethal shock of 25 milliamps. Take the following precautions: Power Supplies Inspect to ensure all switches and indicators are in proper working condition and that power cords and leads are undamaged and properly insulated. Label equipment with warning: Electrical Hazard.” “Danger Connect to ground fault circuit interrupters (GFCI). Use 3-prong plugs. Use power supplies with safety features that detect no-load, overload, sudden load change, short circuit, arc or ground leak, etc. Connecting Leads Turn off main power supply before connecting or disconnecting electrical leads. With dry gloved hands, connect one lead at a time using one hand only. Be sure that plugs are fully seated. Using Equipment
Electrophoresis Standard Operating Procedure Revision Date: 09/14/18 Applies To: Employees responsible for writing lab-specific standard operating procedures; and all faculty, staff, students, and other employees working with electrophoresis. This standard operating procedure (SOP) outlines the process of electrophoresis, including the use of equipment and chemicals necessary for the process. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when performing electrophoresis. [Provide additional information as it pertains to your research protocol] Electrophoresis uses electrical energy to separate molecules based on their size, structure, and electrical charge. [Write the steps for using the chemical in your research protocol] [Provide additional information as it pertains to your research protocol] Electrophoresis equipment can pose significant electrical hazard in the laboratory. Typical electrophoresis units operating at 100 volts can provide a lethal shock of 25 milliamps. Take the following precautions: Power Supplies Inspect to ensure all switches and indicators are in proper working condition and that power cords and leads are undamaged and properly insulated. Label equipment with warning: “Danger Electrical Hazard.” Connect to ground fault circuit interrupters (GFCI). Use 3-prong plugs. Use power supplies with safety features that detect no-load, overload, sudden load change, short circuit, arc or ground leak, etc. Connecting Leads Turn off main power supply before connecting or disconnecting electrical leads. With dry gloved hands, connect one lead at a time using one hand only. Be sure that plugs are fully seated. Using Equipment
Electrophoresis.docx