Figure 8. Photobleaching was performed on an immobile fluorescent sample made using a polyelectolyte gel labeled with Alexa 555. Images show the radial (A, C) and axial profiles (B, D) of the effective bleached volumes for single (A, B) and two-photon excitation (C, D). Images (E) and (F) show the comparison between the experimental bleaching volume (black line) and the corresponding Gaussian approximation (red line) for 1P and 2P excitation, respectively. Inspired by Mazza et al., 2007. Figure 9.The correction constant is shown for a Gaussian spot photobleaching experiment as a function of the bleaching parameter K for both flow and diffusion. Reprinted with permission of Axelrod et al., 1976. Figure 10. A two-dimensional pattern is photobleached by scanning the laser beam with speed v along adjacent line segments that are spaced with a distance The laser intensity is zero outside the Δy. boundaries of the pattern . Reprinted with permission of Braeckmans et al. (2003). Figure 11. (A) A FRAP experiment performed on a solution of FITC-dextran with a molecular weight of 150 kDa. The first image shows the sample before bleaching. The photobleaching of the disk is illustrated in the second image. The subsequent images show the fluorescence recovery. The scale bar represents 20 µm. (B) The fluorescence recovery inside the photobleached disk extracted from the image sequence A (●). best fit of Eq. yields the diffusion coefficient D. Reprinted with permission of Braeckmans et al., 2003. Figure 12. A rectangular area with width and height is photobleached by scanning the laser beam lx ly with speed along adjacent line segments that are spaced with a distance The laser intensity is zero v Δy. outside the rectangular area.
Figure 8. Photobleaching was performed on an immobile fluorescent sample made using a polyelectolyte gel labeled with Alexa 555. Images show the radial (A, C) and axial profiles (B, D) of the effective bleached volumes for single (A, B) and two-photon excitation (C, D). Images (E) and (F) show the comparison between the experimental bleaching volume (black line) and the corresponding Gaussian approximation (red line) for 1P and 2P excitation, respectively. Inspired by Mazza et al., 2007. Figure 9.The correction constant is shown for a Gaussian spot photobleaching experiment as a function of the bleaching parameter K for both flow and diffusion. Reprinted with permission of Axelrod et al., 1976. Figure 10. A two-dimensional pattern is photobleached by scanning the laser beam with speed v along adjacent line segments that are spaced with a distance Δy. The laser intensity is zero outside the boundaries of the pattern . Reprinted with permission of Braeckmans et al. (2003). Figure 11. (A) A FRAP experiment performed on a solution of FITC-dextran with a molecular weight of 150 kDa. The first image shows the sample before bleaching. The photobleaching of the disk is illustrated in the second image. The subsequent images show the fluorescence recovery. The scale bar represents 20 µm. (B) The fluorescence recovery inside the photobleached disk extracted from the image sequence (●). A best fit of Eq. yields the diffusion coefficient D. Reprinted with permission of Braeckmans et al., 2003. Figure 12. A rectangular area with width lx and height ly is photobleached by scanning the laser beam with speed v along adjacent line segments that are spaced with a distance Δy. The laser intensity is zero outside the rectangular area.
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