Method STAGE 1: Setting up the chromatogram Cut enough chromatogram papers from the coffee filter for each of the solvents you are using (plus one for water) - about 5 cm wide and about as tall as your glass Draw pencil lines about 2 cm from one end of your pieces of paper Mix a small amount of the food colouring in about the same volume of water in a small container (e.g. egg cup) Using a fine paint brush, paint small spots of the food colouring mixture onto the pencil lines and gently blow to dry the spots. Repeat once more. Repeat Steps 3 and 4 with the remaining food colourings. STAGE 2: Running the chromatogram Add about 1 cm of water into the glass Using the bulldog clip, attach the top of your paper to the pencil, and lower the paper into the flask ENSURE the top of the water is BELOW the pencil base line. – Allow the water to soak up the paper until about 1 cm from the pencil. Remove the paper from the glass, and mark the highest point of the solvent with a pencil line. Place the chromatogram on a piece of absorbent towel until dry. Repeat stage 2 with the remaining solvents. Figure 1: Experimental set-up for running the paper chromatography Analysis of results Take a picture of the three chromatograms you ran and stick them in below:
Method STAGE 1: Setting up the chromatogram Cut enough chromatogram papers from the coffee filter for each of the solvents you are using (plus one for water) - about 5 cm wide and about as tall as your glass Draw pencil lines about 2 cm from one end of your pieces of paper Mix a small amount of the food colouring in about the same volume of water in a small container (e.g. egg cup) Using a fine paint brush, paint small spots of the food colouring mixture onto the pencil lines and gently blow to dry the spots. Repeat once more. Repeat Steps 3 and 4 with the remaining food colourings. STAGE 2: Running the chromatogram Add about 1 cm of water into the glass Using the bulldog clip, attach the top of your paper to the pencil, and lower the paper into the flask – ENSURE the top of the water is BELOW the pencil base line. Allow the water to soak up the paper until about 1 cm from the pencil. Remove the paper from the glass, and mark the highest point of the solvent with a pencil line. Place the chromatogram on a piece of absorbent towel until dry. Repeat stage 2 with the remaining solvents. Figure 1: Experimental set-up for running the paper chromatography Analysis of results Take a picture of the three chromatograms you ran and stick them in below:
358282-pag-activity-chemistry-separation-techniques-suggestion-3.docx