Skin sensitisation Species Strain, age Sex Route Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference weight information Guinea Pig Hartley M Epidermal 0.5 g dose of test item (undiluted) Results: Ferreira, T.G. Buehler’s closed (Cavia Test material; methiocarb (purity No skin reactions were observed. Also no clinical signs nor 11-July-2013 patch porcellus) behavioural alterations were observed. technique 9 wk 98.2%). (Study/Report-No: RF - (absorbent 7 d gap between inductions (3), & cotton lint) then 14 d to challenge (total: 29 0030.318.279.05 Conclusion: 403-520 g d) Methiocarb is not a skin sensitiser. Document No.: n = 10 M-261825-02-1) Exposure Area = 6 cm2 Exposure Time = 6 h Observation period: 30 d Genotoxicity Species Strains Test parameters (e.g. Vehicle Toxicology conclusion/s Reference information dose) Salmonellla TA 1535, Plate incorporation test: Nutrient Conclusion: Herbold, B. (2007a) typhimurium broth TA 100, 16–5000 µg test material Methiocarb-phenol was considered to be non-mutagenic without and with S9 24-Sept-2007 per plate (usually mix. TA 1537, (Study ID: T 9077 762 Oxoid (LT2 No. 2) TA98, and Report-No: AT04108 mutants) Test material purity: 99.1% TA 102. Document No.: methiocarb phenol M-292915-01-1)
Skin sensitisation Species Strain, age weight Sex Route Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference information Guinea Pig (Cavia porcellus) Hartley 9 wk 403-520 g M Epidermal 0.5 g dose of test item (undiluted) Test material; methiocarb (purity 98.2%). 7 d gap between inductions (3), & then 14 d to challenge (total: 29 d) n = 10 Exposure Area = 6 cm2 Exposure Time = 6 h Observation period: 30 d Buehler’s closed patch technique (absorbent cotton lint) Results: No skin reactions were observed. Also no clinical signs nor behavioural alterations were observed. Conclusion: Methiocarb is not a skin sensitiser. Ferreira, T.G. 11-July-2013 (Study/Report-No: RF - 0030.318.279.05 Document No.: M-261825-02-1) Genotoxicity Species Strains Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference information Salmonellla typhimurium (LT2 mutants) TA 1535, TA 100, TA 1537, TA98, and TA 102. Plate incorporation test: 16–5000 µg test material per plate Test material purity: 99.1% methiocarb phenol Nutrient broth (usually Oxoid No. 2) Conclusion: Methiocarb-phenol was considered to be non-mutagenic without and with S9 mix. Herbold, B. (2007a) 24-Sept-2007 (Study ID: T 9077 762 Report-No: AT04108 Document No.: M-292915-01-1)
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