-S +S 7.31 ± 1.85 11.60 ± 1.38 12 5679 448 52 -S +HS 5.43 ± 1.72 29.19 ± 0.58 24 5537 387 113 -HS +HS S= Significant at 0.05 level, HS= Highly significant at 0.01 level Table 3. Effect of AgNPs (50nm) on mitotic index (MI%) and chromosomal aberration (CA%) of meristems of Vicia faba Total Examined Total Dividing Total Aberrant Duration (hrs) Cells MI (%) CA (%) Cells Cells Control 5836 720 17 12.33 ± 0.73 0.03 ± 0.46 9.80 ± 1.16 5.32 ± 1.13 6 4781 469 21 NS +HS 6.85 ±1.13 12.44 ± 0.97 12 5003 343 27 -HS +HS 3.88± 1.17 34.37 ± 0.81 24 5067 197 33 -HS +HS S= Significant at 0.05 level, HS= Highly significant at 0.01 level Table 4. Effect of AgNPs (20nm) on mitotic index (MI%) and chromosomal aberration (CA%) of meristems of Vicia faba Total Examined Total Dividing Total Aberrant Duration (hrs) Cells MI (%) CA (%) Cells Cells Control 5660 703 23 12.42 ± 1.77 0.12 ± 1.06 6.29 ± 0.97 16.52 ± 0.93 6 5019 316 39 -HS +HS 2.24 ± 1.34 80.72 ± 2.03 12 5213 117 67 -HS +HS 24 5013 00 00 00 00 S= Significant at 0.05 level, HS= Highly significant at 0.01 level, 00= No dividing cells Figure 10. Relationship between nanoparticles- size and percent of chromosomal abnormalities. Figure 11. Disturbed chromosomes at metaphase stage with disoriented chromosomes. Figure 12. Disturbed multipolar anaphase with lagging chromosomes (laggards). Figure 13. Fragment chromosome at metaphase stage (Left). Bridge at ana-telophase stage (Right).
+S -S 11.60 ± 1.38 +HS 7.31 ± 1.85 -S 52 448 5679 12 29.19 ± 0.58 +HS 5.43 ± 1.72 -HS 113 387 5537 24 S= Significant at 0.05 level, HS= Highly significant at 0.01 level Table 3. Effect of AgNPs (50nm) on mitotic index (MI%) and chromosomal aberration (CA%) of meristems of Vicia faba CA (%) MI (%) Total Aberrant Cells Total Dividing Cells Total Examined Cells Duration (hrs) 0.03 ± 0.46 12.33 ± 0.73 17 720 5836 Control 5.32 ± 1.13 +HS 9.80 ± 1.16 NS 21 469 4781 6 12.44 ± 0.97 +HS 6.85 ±1.13 -HS 27 343 5003 12 34.37 ± 0.81 +HS 3.88± 1.17 -HS 33 197 5067 24 S= Significant at 0.05 level, HS= Highly significant at 0.01 level Table 4. Effect of AgNPs (20nm) on mitotic index (MI%) and chromosomal aberration (CA%) of meristems of Vicia faba CA (%) MI (%) Total Aberrant Cells Total Dividing Cells Total Examined Cells Duration (hrs) 0.12 ± 1.06 12.42 ± 1.77 23 703 5660 Control 16.52 ± 0.93 +HS 6.29 ± 0.97 -HS 39 316 5019 6 80.72 ± 2.03 +HS 2.24 ± 1.34 -HS 67 117 5213 12 00 00 00 00 5013 24 S= Significant at 0.05 level, HS= Highly significant at 0.01 level, 00= No dividing cells Figure 10. Relationship between nanoparticles- size and percent of chromosomal abnormalities. Figure 11. Disturbed chromosomes at metaphase stage with disoriented chromosomes. Figure 12. Disturbed multipolar anaphase with lagging chromosomes (laggards). Figure 13. Fragment chromosome at metaphase stage (Left). Bridge at ana-telophase stage (Right).
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