Western blotting Item Supplier Phosphate Buffered Saline (PBS) - OXOID tablets Tween 20 Fisher Scientific Cell Signalling Biotinylated Protein Ladder Detection Precision Plus Protein Standards Bio Rad Immobilon Transfer Membrane Millipore (PVDF membrane) SuperSignal®West Dura Thermo Scientific Extended Duration Substrate Mini-PROTEAN Tetra Cell BioRad Mini Trans-Blot® Electrophoretic BioRad Transfer Cell Film Hyperfilm™ Amersham Developer AGAFA Fixer GS-900™ Calibrated Densitometer BioRad 1.5 mm cast plates (Bio RAD) Materials used in the experiment Tris/Glycine 10X Amount per Litre Supplier Item Tris 250mM 30.3g Fisher Scientific Glycine 1.92M 144.1g Fisher Scientific Deionised water Up to 1 Litre - Materials used in the experiment Running Buffer Item Volume Supplier 10% SDS 20 ml Sigma Aldrich 200 ml - Tris/Glycine 10X Deionised water Up to 2 Litres - Materials used in the experiment
Western blotting Item Supplier Phosphate Buffered Saline (PBS) - tablets OXOID Tween 20 Fisher Scientific Biotinylated Protein Ladder Detection Cell Signalling Precision Plus Protein Standards Bio Rad Immobilon Transfer Membrane (PVDF membrane) Millipore SuperSignal®West Dura Extended Duration Substrate Thermo Scientific Mini-PROTEAN Tetra Cell BioRad Mini Trans-Blot® Electrophoretic Transfer Cell BioRad Film Hyperfilm™ Amersham Developer AGAFA Fixer GS-900™ Calibrated Densitometer BioRad 1.5 mm cast plates (Bio RAD) Materials used in the experiment Tris/Glycine 10X Item Amount per Litre Supplier Tris 250mM 30.3g Fisher Scientific Glycine 1.92M 144.1g Fisher Scientific Deionised water Up to 1 Litre - Materials used in the experiment Running Buffer Item Volume Supplier 10% SDS 20 ml Sigma Aldrich Tris/Glycine 10X 200 ml - Deionised water Up to 2 Litres - Materials used in the experiment
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