Risk assessment COSHH risk assessment - University of Oxford COSHH Assessment Form Description of procedure Substances used Culture of eye specimens Variable, depending on organism cultured (may include Gram stain reagents; 3% hydrogen peroxide (catalase test); (oxidase test); sodium deoxycholate (bile solubility test); bioMerieux API reagents) Quantities of chemicals used Frequency of SOP use Daily Hazards identified Could a less hazardous substance be 1. Autoclaved liquid used instead? 2. Potentially infectious material in sample No 3. Potentially pathogenic bacteria 4. Chemical exposure form bacterial identification tests What measures have you to control risk? 1. Training in good laboratory practices (GLP) 2. Appropriate PPE (lab coat, gloves, eye protection) 3. Use of biosafety cabinet for reading of plates / follow-up of BSL-3 organisms (e.g. B. pseudomallei) Checks on control measures Observation and supervision by senior staff Is health surveillance required? Training requirements: No GLP Emergency procedures: Waste disposal procedures: 1. Report all incidents to Safety Adviser 1. Sharps discarded into appropriate rigid 2. Use eyewash for splashes containers for incineration 3. Clean up spills using 1% Virkon or 2. Infectious waste discarded into autoclave bags chemical spill kit or 1% Virkon solution prior to autoclaving and subsequent incineration 3. Chemical waste disposed of according to manufacturer’s instructions
Risk assessment COSHH risk assessment - University of Oxford COSHH Assessment Form Description of procedure Culture of eye specimens Substances used Variable, depending on organism cultured (may include Gram stain reagents; 3% hydrogen peroxide (catalase test); (oxidase test); sodium deoxycholate (bile solubility test); bioMerieux API reagents) Quantities of chemicals used Frequency of SOP use Daily Hazards identified 1. Autoclaved liquid 2. Potentially infectious material in sample 3. Potentially pathogenic bacteria 4. Chemical exposure form bacterial identification tests Could a less hazardous substance be used instead? No What measures have you to control risk? 1. Training in good laboratory practices (GLP) 2. Appropriate PPE (lab coat, gloves, eye protection) 3. Use of biosafety cabinet for reading of plates / follow-up of BSL-3 organisms (e.g. B. pseudomallei) Checks on control measures Observation and supervision by senior staff Is health surveillance required? No Training requirements: GLP Emergency procedures: 1. Report all incidents to Safety Adviser 2. Use eyewash for splashes 3. Clean up spills using 1% Virkon or chemical spill kit Waste disposal procedures: 1. Sharps discarded into appropriate rigid containers for incineration 2. Infectious waste discarded into autoclave bags or 1% Virkon solution prior to autoclaving and subsequent incineration 3. Chemical waste disposed of according to manufacturer’s instructions
Culture_Eye_Specimens.docx