Directions- Protease peptone 3.0 gm Suspend 56.53 Gms in 1000 ml distilled Lactose 10.0 gm water. Bile salt 1.5 gm Heat to boiling to dissolve the medium NaCl 5.0 gm completely. Neutral red 0.03 gm Sterilize by autoclaving at 15 lbs pressure Agar 20.0 gm (121°C) for 15 minutes. Final pH 7.1 ± 0.2 Nutrient Agar Media:- Directions- Components Amt. (Gms/Lit.) Suspend 38 gms in 1000 ml distilled water. Peptone 10 gm Heat to boiling to dissolve the medium NaCl 5 gm completely. Beef Extract 3 gm Sterilize by autoclaving at 15 lbs pressure Agar 20 gm (121°C) for 15 minutes. Final pH 7.4 ± 0.2 Nutrient Gelatin broth:- Directions- Components Amt. (Gms/Lit.) Suspend 163.0 Gms in 1000 ml distilled Meat extract 3.0 gm water. Peptone 10.0 gm Heat to boiling to dissolve the medium Gelatin 150.0 gm completely. D/W 1000.0 ml Sterilize by autoclaving at 15 lbs pressure Final pH 7.2 (121°C) for 15 minutes. Nutrient sugar Broth:- Components Amt. 1% Peptone 90 ml
Protease peptone 3.0 gm Lactose 10.0 gm Bile salt 1.5 gm NaCl 5.0 gm Neutral red 0.03 gm Agar 20.0 gm Final pH 7.1 ± 0.2 Directions- Suspend 56.53 Gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Nutrient Agar Media:- Components Amt. (Gms/Lit.) Peptone 10 gm NaCl 5 gm Beef Extract 3 gm Agar 20 gm Final pH 7.4 ± 0.2 Directions- Suspend 38 gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Nutrient Gelatin broth:- Components Amt. (Gms/Lit.) Meat extract 3.0 gm Peptone 10.0 gm Gelatin 150.0 gm D/W 1000.0 ml Final pH 7.2 Directions- Suspend 163.0 Gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Nutrient sugar Broth:- Components Amt. 1% Peptone 90 ml
Dissertation-on-Petroleum-Biodegradation-In-Natural-Environment.docx