2 mls of 24hr conditioned phenol-red free/serum-free media were assayed for uPA. Fig 5.3 shows uPA activity in the three cell lines in conditioned media from cells cultured on fibrin gel with the highest uPA activity in the epithelial-like cells (NRK52E) followed by the mesangial cells (CRL2573) and the fibroblast like cells (NRK49F). The figure also compares uPA activity between the cells cultured on fibrin with those cultured on plastic. A significant uPA activity was observed in cells. Figure 5.3 uPA Activity p-EGR-pNA in conditioned medium from rat kidney cells cultured on both fibrin gel and plastic. uPA activity in conditioned media in the presence and absence of fibrin. The data shows uPA activity per hour. Individual points were in triplicate and n=3. The difference of uPA activities between cells cultured on fibrin and plastic in NRK52E was statistically significant at P< 0.0001. 5.3 Determination of CPB2 activity in NRK52E, NRK49F and CRL2573 cells. CPB2 activity was assessed in all our cell lines (NRK52E, NRK49F and CRL2573) using 24hr conditioned phenol-red free/serum-free media with the Pefakit substrate (Section 2.7.7). Fig 5.4 shows CPB2 activity in conditioned media from cells cultured on both fibrin and plastic. The cell lines generally showed more activity when cultured on fibrin. The highest CPB2 activity was in epithelial-like cells cultured on fibrin with a significant difference of P< 0.0001. Figure 5.4 CPB2 Activity in conditioned medium from rat kidney cells cultured on fibrin and plastic. CPB2 activity in conditioned media from cells cultured on fibrin and plastic were compared. The data show CPB2 activity per hour. The data are means +/- SEM of results from 3 different cell cultures. The difference of CPB2 activities between NRK52E cells cultured on fibrin and
2 mls of 24hr conditioned phenol-red free/serum-free media were assayed for uPA. Fig 5.3 shows uPA activity in the three cell lines in conditioned media from cells cultured on fibrin gel with the highest uPA activity in the epithelial-like cells (NRK52E) followed by the mesangial cells (CRL2573) and the fibroblast like cells (NRK49F). The figure also compares uPA activity between the cells cultured on fibrin with those cultured on plastic. A significant uPA activity was observed in cells. Figure 5.3 uPA Activity p-EGR-pNA in conditioned medium from rat kidney cells cultured on both fibrin gel and plastic. uPA activity in conditioned media in the presence and absence of fibrin. The data shows uPA activity per hour. Individual points were in triplicate and n=3. The difference of uPA activities between cells cultured on fibrin and plastic in NRK52E was statistically significant at P< 0.0001. 5.3 Determination of CPB2 activity in NRK52E, NRK49F and CRL2573 cells. CPB2 activity was assessed in all our cell lines (NRK52E, NRK49F and CRL2573) using 24hr conditioned phenol-red free/serum-free media with the Pefakit substrate (Section 2.7.7). Fig 5.4 shows CPB2 activity in conditioned media from cells cultured on both fibrin and plastic. The cell lines generally showed more activity when cultured on fibrin. The highest CPB2 activity was in epithelial-like cells cultured on fibrin with a significant difference of P< 0.0001. Figure 5.4 CPB2 Activity in conditioned medium from rat kidney cells cultured on fibrin and plastic. CPB2 activity in conditioned media from cells cultured on fibrin and plastic were compared. The data show CPB2 activity per hour. The data are means +/- SEM of results from 3 different cell cultures. The difference of CPB2 activities between NRK52E cells cultured on fibrin and
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