Supplementary information Supplementary information: Primary structure stability of collagen SDS PAGE – L- Standard protein ladder C+MN Collagen applied with microneedle pre-treatment – C-MN Collagen applied on native skin without microneedle pre-treatment – FC Freshly prepared solution of collagen – B Buffer applied on the skin with microneedle pre-treatment – It was observed that collagen showed characteristic doublet peaks at 235-215 kDA and 130-115 kDa, both in freshly prepared solutions as well as collagen applied on skin with or without microneedle pre-treatment. For collagen applied on skin with microneedle pre-treatment, another singlet band was observed around 72 kDa, which was similar to a band observed when only solvent (no collagen) and could be attributed to some skin proteins.
Supplementary information Supplementary information: Primary structure stability of collagen – SDS PAGE L- Standard protein ladder C+MN – Collagen applied with microneedle pre-treatment C-MN – Collagen applied on native skin without microneedle pre-treatment FC – Freshly prepared solution of collagen B – Buffer applied on the skin with microneedle pre-treatment It was observed that collagen showed characteristic doublet peaks at 235-215 kDA and 130-115 kDa, both in freshly prepared solutions as well as collagen applied on skin with or without microneedle pre-treatment. For collagen applied on skin with microneedle pre-treatment, another singlet band was observed around 72 kDa, which was similar to a band observed when only solvent (no collagen) and could be attributed to some skin proteins.
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