DNA Profiling Review Purpose of understanding population genetics (allele frequency) for Forensic Science Gel Electrophoresis how does it separate DNA fragments (charges) – # alleles an individual would have of a particular gene or locus Differentiate between homozygous and heterozygous genotype on an electropherogram (STR analysis) understand how to determine #repeats and # of base pairs looking at an electropherogram for a STR locus What are flanking regions for STR loci and what is their importance for PCR? How do you determine the # of base of a locus? Flanking region + repeat sequence x # repeats Differentiate between coding & noncoding regions and determine which are of importance to DNA profiling Describe the AMEL location and its purpose What do the peaks on an electropherogram show? Controversy surrounding familial DNA Compare and contrast mtDNA and YSTR analysis Action of restriction enzymes, how they know where to cut, how many fragments are made from a certain number of cuts, how these fragments show up on a gel (identify smaller and larger fragments, match two samples of DNA that are identical) What is a polymorphism? Differentiate between what a DNA sample would look like on a gel if it had been fragmented or not by restriction enzymes Differentiate between RFLP and STR analysis Kirk Bloodsworth, Steven Avery, Grim Sleeper, Romanovs, Karen Klaas
DNA Profiling Review Purpose of understanding population genetics (allele frequency) for Forensic Science Gel Electrophoresis – how does it separate DNA fragments (charges) # alleles an individual would have of a particular gene or locus Differentiate between homozygous and heterozygous genotype on an electropherogram (STR analysis) understand how to determine #repeats and # of base pairs looking at an electropherogram for a STR locus What are flanking regions for STR loci and what is their importance for PCR? How do you determine the # of base of a locus? Flanking region + repeat sequence x # repeats Differentiate between coding & noncoding regions and determine which are of importance to DNA profiling Describe the AMEL location and its purpose What do the peaks on an electropherogram show? Controversy surrounding familial DNA Compare and contrast mtDNA and YSTR analysis Action of restriction enzymes, how they know where to cut, how many fragments are made from a certain number of cuts, how these fragments show up on a gel (identify smaller and larger fragments, match two samples of DNA that are identical) What is a polymorphism? Differentiate between what a DNA sample would look like on a gel if it had been fragmented or not by restriction enzymes Differentiate between RFLP and STR analysis Kirk Bloodsworth, Steven Avery, Grim Sleeper, Romanovs, Karen Klaas
dna_profiling_review.docx