Any organism considered to be a contaminant may not require full species level identification significant isolates at -80C in a cryovial containing 1ml STGG and record the isolate details in the freezer logbook/database. Antimicrobial susceptibility testing All significant isolates should have antimicrobial susceptibilities performed according to SOP Direct susceptibility testing may be performed in certain circumstances (i.e. to improve turnaround time), although these results will need to be repeated if confluent growth is not obtained or the culture is mixed. For GNB, add 1-2 drops of blood culture broth to 2ml of sterile saline and use this as the inoculum for preparation of antimicrobial susceptibility testing media. A greater inoculum (or a drop of blood culture broth applied directly to the agar plate) may be required for Gram positive organisms. Reporting Gram stain: report organism detected (by telephone / urgent ward review + hard copy). Culture: All organisms isolated (with comment if isolate(s) are of doubtful significance) Absence of growth Interim reports growth at 24 hours” (based on no growth on 24 hour blind sub-culture) “No growth at 48 hours” (based on negative 24 hour blind sub-culture “No AND clear broth at 48 hour inspection) Final report: growth at 7 days” “No Do not use the terms significant growth” or growth of doubtful significance” “no “mixed Antimicrobial susceptibility testing: report as clinically indicated. Quality assurance Media and identification tests should be quality controlled according to the relevant MOPSOP. Limitations Several factors may result in a negative blood culture, including:
Any organism considered to be a contaminant may not require full species level identification significant isolates at -80C in a cryovial containing 1ml STGG and record the isolate details in the freezer logbook/database. Antimicrobial susceptibility testing All significant isolates should have antimicrobial susceptibilities performed according to SOP Direct susceptibility testing may be performed in certain circumstances (i.e. to improve turnaround time), although these results will need to be repeated if confluent growth is not obtained or the culture is mixed. For GNB, add 1-2 drops of blood culture broth to 2ml of sterile saline and use this as the inoculum for preparation of antimicrobial susceptibility testing media. A greater inoculum (or a drop of blood culture broth applied directly to the agar plate) may be required for Gram positive organisms. Reporting Gram stain: report organism detected (by telephone / urgent ward review + hard copy). Culture: All organisms isolated (with comment if isolate(s) are of doubtful significance) Absence of growth Interim reports “No growth at 24 hours” (based on no growth on 24 hour blind sub-culture) “No growth at 48 hours” (based on negative 24 hour blind sub-culture AND clear broth at 48 hour inspection) Final report: “No growth at 7 days” Do not use the terms “no significant growth” or “mixed growth of doubtful significance” Antimicrobial susceptibility testing: report as clinically indicated. Quality assurance Media and identification tests should be quality controlled according to the relevant MOPSOP. Limitations Several factors may result in a negative blood culture, including:
Culture_of_Blood.docx