1 Bacteria K12, 1 Non-conjugative Escherichia coli E. coli B, C or Nissle plasmids E. coli E. coli 1917—any derivative that 2 Bacteriophage— does not contain— (a) lambda (a) generalised transducing (b) lambdoid phages; or (c) Fd or F1 (b) genes able to (e.g. M13) complement the 3 None (non-vector conjugation defect in a systems) non-conjugative plasmid species 1 Non-conjugative Bacillus—specified strains with a plasmids —asporogenic reversion frequency of less 2 Plasmids and than 10–7— phages whose host (a) range does not B. amyloliquefaciens include B. cereus, (b) B. licheniformis or B. anthracis (c) B. pumilus any other (d) B. subtilis pathogenic strain (e) of B. thuringiensis Bacillus 3 None (non-vector systems) 1 Non-conjugative Pseudomonas putida—strain KT 2440 plasmids including certified plasmids: pKT 262, pKT 263, pKT 264 2 None (non-vector systems) Sch. 2
1 Bacteria Escherichia coli K12, E. coli B, E. coli C or E. coli Nissle 1917—any derivative that does not contain— (a) generalised transducing phages; or (b) genes able to complement the conjugation defect in a non-conjugative plasmid 1 Non-conjugative plasmids 2 Bacteriophage— (a) lambda (b) lambdoid (c) Fd or F1 (e.g. M13) 3 None (non-vector systems) Bacillus—specified species —asporogenic strains with a reversion frequency of less than 10–7— (a) B. amyloliquefaciens (b) B. licheniformis (c) B. pumilus (d) B. subtilis (e) B. thuringiensis 1 Non-conjugative plasmids 2 Plasmids and phages whose host range does not include B. cereus, B. anthracis or any other pathogenic strain of Bacillus 3 None (non-vector systems) Pseudomonas putida—strain KT 2440 1 Non-conjugative plasmids including certified plasmids: pKT 262, pKT 263, pKT 264 2 None (non-vector systems) Sch. 2
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