Culture media, conditions, and target organisms Table 1. Swabs Clinical / Gram Standard media Incubation Cultures read Target organism(s) strain Temp (°C) Atmosphere Time All Blood agar 35 37 5 10% CO2 40 - 48h Daily streptococci – – β-haemolytic Pasteurella spp. (bite wounds) S. aureus Vibrio spp. Chocolate agar 35 37 5 10% CO2 40 - 48h Daily Haemophilus spp. – – CNA-blood agar 35 37 Air 40 - 48h Daily streptococci – β-haemolytic S. aureus MacConkey agar 35 37 Air 40 - 48h Daily Enterobacteriaceae – Pseudomonads If yeast or fungal Sabouraud agar 35 37 Air 40 - 48h Daily Fungi – elements seen If ?melioid or ASH 35 37 Air 48h (Sub B. pseudomallei – SB to ASH parotitis SB at 48h)
Table 1. Culture media, conditions, and target organisms Swabs Clinical / Gram strain Standard media Incubation Cultures read Target organism(s) Temp (°C) Atmosphere Time All Blood agar 35 – 37 5 – 10% CO2 40 - 48h Daily β-haemolytic streptococci Pasteurella spp. (bite wounds) S. aureus Vibrio spp. Chocolate agar 35 – 37 5 – 10% CO2 40 - 48h Daily Haemophilus spp. CNA-blood agar 35 – 37 Air 40 - 48h Daily β-haemolytic streptococci S. aureus MacConkey agar 35 – 37 Air 40 - 48h Daily Enterobacteriaceae Pseudomonads If yeast or fungal elements seen Sabouraud agar 35 – 37 Air 40 - 48h Daily Fungi If ?melioid or parotitis ASH SB 35 – 37 Air 48h (Sub SB to ASH at 48h) B. pseudomallei
Culture_of_Pus_Swabs.docx