No cost/benefit analysis on Perform applied R&D on the mechanical front-end needs to the advantages to increasing focus on fractionation (i.e. clean lignin removal) and the or decreasing pretreatment production of a highly concentrated C5/C6 sugar stream severity exists Define the effects of increased xylan concentration in unit operations and associated cost impacts Develop new (fundamental) methods for species-selective adsorption during integrated cleanup Barrier Area 3: Enzyme Science Research Activities & Biotechnology The mechanistic basis Fulfill need for increased substrate structure chemistry R&D underlying the action of most (characterize enzymes interaction and changes during hydrolytic enzymes are still digestion) largely misunderstood Investigate integrating thermochemical/thermomechanical Poor categorization and pretreatment with enzymatic saccharification understanding of the natural Design new cellulase enzymes with enhanced diversity in hydrolytic thermostability and pH tolerance enzymes Perform fundamental studies to ascertain why glycans and The advantages and C6 oligomers decrease cellulase activity disadvantages of using Design new hydrolytic enzymes to work synergistically in lignolytic enzyme systems mild pretreatment conditions have received relatively little Focus on applied R&D for enzyme reuse/recycle in R&D focus industrial conditions The specific activity of hydrolytic enzymes are typically low Critical need for understanding applied processes (not basic science) End products (sugars and degradation products) inhibit enzymes, preventing high sugar concentrations Lignin derived species hinder enzyme efficiencies Cellulase enzyme loading with high solids (>20% w/w) is cost prohibitive
No cost/benefit analysis on the advantages to increasing or decreasing pretreatment severity exists Perform applied R&D on the mechanical front-end needs to focus on fractionation (i.e. clean lignin removal) and the production of a highly concentrated C5/C6 sugar stream Define the effects of increased xylan concentration in unit operations and associated cost impacts Develop new (fundamental) methods for species-selective adsorption during integrated cleanup Barrier Area 3: Enzyme Science & Biotechnology Research Activities The mechanistic basis underlying the action of most hydrolytic enzymes are still largely misunderstood Poor categorization and understanding of the natural diversity in hydrolytic enzymes The advantages and disadvantages of using lignolytic enzyme systems have received relatively little R&D focus The specific activity of hydrolytic enzymes are typically low Critical need for understanding applied processes (not basic science) End products (sugars and degradation products) inhibit enzymes, preventing high sugar concentrations Lignin derived species hinder enzyme efficiencies Cellulase enzyme loading with high solids (>20% w/w) is cost prohibitive Fulfill need for increased substrate structure chemistry R&D (characterize enzymes interaction and changes during digestion) Investigate integrating thermochemical/thermomechanical pretreatment with enzymatic saccharification Design new cellulase enzymes with enhanced thermostability and pH tolerance Perform fundamental studies to ascertain why glycans and C6 oligomers decrease cellulase activity Design new hydrolytic enzymes to work synergistically in mild pretreatment conditions Focus on applied R&D for enzyme reuse/recycle in industrial conditions
CTAB%20ROADMAP_PreRFI_Final.docx