Culture media, conditions, and target organisms Table 1. Swabs Clinical / Gram Standard media Incubation Cultures read Target organism(s) strain Temp (°C) Atmosphere Time All Blood agar 35 37 5 10% CO2 40 - 48h streptococci – – β-haemolytic S. aureus Chocolate agar 35 37 5 10% CO2 40 - 48h Daily – – spp. Haemophilus Neisseria spp. S. pneumoniae Yeasts MacConkey agar 35 37 Air 40 - 48h Daily Enterobacteriaceae – Pseudomonads Neonate GC agar 35 37 5 10% CO2 40 - 48h Daily N. gonorrhoeae – – Corneal scraping or pus / intra-ocular fluid
Table 1. Culture media, conditions, and target organisms Swabs Clinical / Gram strain Standard media Incubation Cultures read Target organism(s) Temp (°C) Atmosphere Time All Blood agar 35 – 37 5 – 10% CO2 40 - 48h β-haemolytic streptococci S. aureus Haemophilus spp. Neisseria spp. S. pneumoniae Yeasts Chocolate agar 35 – 37 5 – 10% CO2 40 - 48h Daily MacConkey agar 35 – 37 Air 40 - 48h Daily Enterobacteriaceae Pseudomonads Neonate GC agar 35 – 37 5 – 10% CO2 40 - 48h Daily N. gonorrhoeae Corneal scraping or pus / intra-ocular fluid
Culture_Eye_Specimens.docx