Commercial Plates In Plates Isolates No versus ITC VRC PSC Drug-free ITC VRC PSC Drug-free growth All isolates Score versus 91% 83% 67% 100% 86% 85% 69% 100% ≤0 >0 Score versus 90% 88% 95% 100% 90% 91% 90% 100% ≤1 >1 Score versus 74% 95% 87% 97% 68% 95% 87% 94% ≤2 >2 Wild-type Score versus 98% 98% 98% 98% 93% 100% 100% 98% ≤0 >0 Score versus 100% 100% 100% 98% 100% 100% 100% 98% ≤1 >1 Score versus 100% 100% 100% 95% 100% 100% 100% 90% ≤2 >2 CYP mutants Score versus 83% 68% 38% 98% 78% 68% 40% 98% ≤0 >0 Score versus 80% 75% 90% 98% 80% 80% 80% 98% ≤1 >1 Score versus 50% 88% 75% 95% 38% 88% 75% 93% ≤2 >2 Table S3. Sensitivity and specificity based on presence of CYP mutations and growth score versus >1 in at least 1 azole containing agar well out of ≤1 all thee azoles (itraconazole, posaconazole and voriconazole) and out of two of the azoles (itraconazole/voriconazole, or itraconazole/posaconazole or voriconazole/posaconazole, respectively). Commercial Plates - Observer Commercial Plates - Observer Laboratory In house Plates - Observer 1 In house Plates - Observer 2 1 2
Commercial Plates In Plates Isolates No versus growth ITC VRC PSC Drug-free ITC VRC PSC Drug-free All isolates Score ≤0 versus >0 91% 83% 67% 100% 86% 85% 69% 100% Score ≤1 versus >1 90% 88% 95% 100% 90% 91% 90% 100% Score ≤2 versus >2 74% 95% 87% 97% 68% 95% 87% 94% Wild-type Score ≤0 versus >0 98% 98% 98% 98% 93% 100% 100% 98% Score ≤1 versus >1 100% 100% 100% 98% 100% 100% 100% 98% Score ≤2 versus >2 100% 100% 100% 95% 100% 100% 100% 90% CYP mutants Score ≤0 versus >0 83% 68% 38% 98% 78% 68% 40% 98% Score ≤1 versus >1 80% 75% 90% 98% 80% 80% 80% 98% Score ≤2 versus >2 50% 88% 75% 95% 38% 88% 75% 93% Table S3. Sensitivity and specificity based on presence of CYP mutations and growth score ≤1 versus >1 in at least 1 azole containing agar well out of all thee azoles (itraconazole, posaconazole and voriconazole) and out of two of the azoles (itraconazole/voriconazole, or itraconazole/posaconazole or voriconazole/posaconazole, respectively). Laboratory Commercial Plates - Observer 1 In house Plates - Observer 1 Commercial Plates - Observer 2 In house Plates - Observer 2
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