brought the parasite with it from its native range. has been reported T. contejeani from a number of crayfish hosts (Graham and France 1986; Quilter 1976), and there is a single report of from in its native range in California T. contejeani P. leniusculus (McGriff and Modin 1983); but identification is based on spore size, and molecular or ultrastructural confirmation is lacking. The pattern of infection in the current study leads us to suggest that it is more likely that in the UK has acquired P. leniusculus T. from the native host. was detected in only sites, contejeani T. contejeani A. pallipes in mixed sites and in sites where only occurred. Furthermore, identical P. leniusculus sequences were found in the native and invading species (Table 4). These data fit a pattern of transmission from the native to the invading species in mixed A. pallipes sites. Detailed studies of the fitness effects of and its mode of T. contejeani transmission within and between crayfish species are required. In addition, our sequences had 98-100 % sequence similarity to T. contejeani the unclassified microsporidium, sp. JES2002H, which was detected Microsporidium in three species of amphipod in France (Terry et al. 2004). We suggest that sp. JES2002H and may be the same species, although Microsporidium T. contejeani confirmation awaits ultrastructural analysis of sp. JES2002H. Microsporidium In one we found a microsporidium sequence with 97 % sequence P. leniusculus similarity to a parasite previously described from the Bacillidium vesiculoformis, oligochaete worm in Scotland, UK (Morris et al. 2005). The sequence Nais simplex similarity indicates that the parasite is likely to be in the same genus as B. however further molecular and morphological analysis would be vesiculoformis; required to confirm this. This is the first record of a spp. in crayfish and Bacillidium
brought the parasite with it from its native range. T. contejeani has been reported from a number of crayfish hosts (Graham and France 1986; Quilter 1976), and there is a single report of T. contejeani from P. leniusculus in its native range in California (McGriff and Modin 1983); but identification is based on spore size, and molecular or ultrastructural confirmation is lacking. The pattern of infection in the current study leads us to suggest that it is more likely that P. leniusculus in the UK has acquired T. contejeani from the native host. T. contejeani was detected in A. pallipes only sites, in mixed sites and in sites where only P. leniusculus occurred. Furthermore, identical sequences were found in the native and invading species (Table 4). These data fit a pattern of transmission from the native A. pallipes to the invading species in mixed sites. Detailed studies of the fitness effects of T. contejeani and its mode of transmission within and between crayfish species are required. In addition, our T. contejeani sequences had 98-100 % sequence similarity to the unclassified microsporidium, Microsporidium sp. JES2002H, which was detected in three species of amphipod in France (Terry et al. 2004). We suggest that Microsporidium sp. JES2002H and T. contejeani may be the same species, although confirmation awaits ultrastructural analysis of Microsporidium sp. JES2002H. In one P. leniusculus we found a microsporidium sequence with 97 % sequence similarity to Bacillidium vesiculoformis, a parasite previously described from the oligochaete worm Nais simplex in Scotland, UK (Morris et al. 2005). The sequence similarity indicates that the parasite is likely to be in the same genus as B. vesiculoformis; however further molecular and morphological analysis would be required to confirm this. This is the first record of a Bacillidium spp. in crayfish and
Crayfish%20paper%2021.01.08%20second%20revision.docx