Table 1. Culture media, conditions, and target organisms Clinical / Gram Standard media Incubation Cultures read Target organism(s) strain Temp (°C) Atmosphere Time All Blood agar + 35 37 5 10% CO2 40 - 48h H. influenzae, M. catarrhalis, S. aureus, – – optochin disc S. pneumoniae, Beta-haemolytic streptococci Chocolate agar 35 37 5 10% CO2 40 - 48h Daily – – Other organisms in pure growth may be significant If ICU patient or MacConkey agar 35 37 Air 40 - 48h Daily Enterobacteriaceae – suspected Pseudomonads infection If yeast or fungal Sabouraud agar 35 37 Air 40 - 48h Daily Fungi – elements seen or suspected fungal infection If ?melioid ASH 35 37 Air 48h (Sub Daily B. pseudomallei – SB to ASH SB at 48h)
Table 1. Culture media, conditions, and target organisms Clinical / Gram strain Standard media Incubation Cultures read Target organism(s) Temp (°C) Atmosphere Time All Blood agar + optochin disc 35 – 37 5 – 10% CO2 40 - 48h H. influenzae, M. catarrhalis, S. aureus, S. pneumoniae, Beta-haemolytic streptococci Other organisms in pure growth may be significant Chocolate agar 35 – 37 5 – 10% CO2 40 - 48h Daily If ICU patient or suspected infection MacConkey agar 35 – 37 Air 40 - 48h Daily Enterobacteriaceae Pseudomonads If yeast or fungal elements seen or suspected fungal infection Sabouraud agar 35 – 37 Air 40 - 48h Daily Fungi If ?melioid ASH SB 35 – 37 Air 48h (Sub SB to ASH at 48h) Daily B. pseudomallei
Culture_Sputum_ETT_Aspirate.docx