Chapter-6 APPENDIXES Appendix-1 Culture media: Bushnell-Haas Media:- Components Amt. (Gms/Lit.) Directions- Suspend 3.270 grams in 1000 ml distilled MgSo4 0.200 gm water. CaCl2 0.020 gm Heat to boiling to dissolve the medium K2HPO4 1.0 gm completely. KH2PO4 1.0 gm Sterilize by autoclaving at 15 lbs pressure Ammonium Nitrate 1.0 gm (121°C) for 15 minutes. Ferric Chloride 0.050 gm Take 990 ml BHM +10 ml Hydrocarbon Final pH 7.0 ± 0.2 source (Oil, Petrol etc.) . Glucose Phosphate Broth:- Directions- Components Amt. (Gms/Lit.) Suspend 15 in 1000 ml distilled water. Glucose 5.0 gm Heat to boiling to dissolve the medium K2HPO4 5.0 gm completely. Peptone 5.0 gm Sterilize by autoclaving at 15 lbs pressure D/W 1000 ml (121°C) for 15 minutes. Final pH 6.9-7.0 Macconkeys Agar Media:- Components Amt. (Gm/Lit.) Peptone 17.0 gm
Chapter-6 APPENDIXES Appendix-1 Culture media: Bushnell-Haas Media:- Components Amt. (Gms/Lit.) MgSo4 0.200 gm CaCl2 0.020 gm K2HPO4 1.0 gm KH2PO4 1.0 gm Ammonium Nitrate 1.0 gm Ferric Chloride 0.050 gm Final pH 7.0 ± 0.2 Directions- Suspend 3.270 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Take 990 ml BHM +10 ml Hydrocarbon source (Oil, Petrol etc.) . Glucose Phosphate Broth:- Components Amt. (Gms/Lit.) Glucose 5.0 gm K2HPO4 5.0 gm Peptone 5.0 gm D/W 1000 ml Final pH 6.9-7.0 Directions- Suspend 15 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Macconkeys Agar Media:- Components Amt. (Gm/Lit.) Peptone 17.0 gm
Dissertation-on-Petroleum-Biodegradation-In-Natural-Environment.docx