PHB Lipopolysaccharide binding protein PhaP phasin In vitro 52 removal Endotoxin 2. Comparison of PHA nanoparticles in vitro and in vivo production process, their applications and costs. In vivo In vitro Ref. Production by bacteria Synthetic production 2,20 Use of renewable sources for Harsh chemical needed for polymer 30,53 production isolation and particle production Simultaneous production and Functionalization posterior to functionalization nanobead production Nanobead assembly and disassembly control over bead assembly and 10,54 processing cannot be tightly controlled disassembly and Competition of recombinant and wild Functionalization with target protein type GAPs only, no other GAPs Production Particle size can be controlled by Tight control over particle size 32,54 biotechnological production process Immobilized protein concentration control over immobilized 7,30 variation might represent challenge protein concentration
Endotoxin removal PHB Lipopolysaccharide binding protein PhaP phasin In vitro 52 2. Comparison of PHA nanoparticles in vitro and in vivo production process, their applications and costs. In vivo In vitro Ref. Production and processing Production by bacteria Synthetic production 2,20 Use of renewable sources for production Harsh chemical needed for polymer isolation and particle production 30,53 Simultaneous production and functionalization Functionalization posterior to nanobead production Nanobead assembly and disassembly cannot be tightly controlled control over bead assembly and disassembly 10,54 Competition of recombinant and wild type GAPs Functionalization with target protein only, no other GAPs Particle size can be controlled by biotechnological production process Tight control over particle size 32,54 Immobilized protein concentration variation might represent challenge control over immobilized protein concentration 7,30
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