No. polished high-quality 5,786 1 Quality isoformsD Filtering No. polished low-quality (Quiver) 5,989 isoforms A of read numbers to total No. reads B No. reads containing 5’-, 3’- and poly(A) reads altogether C No. containing PHQI, PLQIs D No. misread-corrected polished with consensus isoforms Table S4. The comparison process of obtaining polished high-quality isoforms (PHQIs) from the total reads between 8 and 16 SMRT cells. The PHQIs were generated using IsoSeqTM (SMRT® Portal version 2.2.0) No. (isoforms) Structure/ Classification processing 8 cells 16 No. consensus isoforms 63,737 113,316 Consensus calling (ICE) Average length of consensus 2,610 2,645 isoforms No. polished high-quality 5,786 10,449 Quality isoforms Filtering No. polished low-quality (Quiver) 5,989 8,800 isoforms Table S5. Production of PHQIs and average length from each tissue: flower, leaf, bulb and root. Due to overlapped gene expression among the tissues, total reads from the four tissues were combined to
Quality Filtering (Quiver) No. polished high-quality isoformsD 5,786 1 No. polished low-quality isoforms 5,989 A of read numbers to total No. reads B No. reads containing 5’-, 3’- and poly(A) reads altogether C No. containing PHQI, PLQIs D No. misread-corrected polished with consensus isoforms Table S4. The comparison process of obtaining polished high-quality isoforms (PHQIs) from the total reads between 8 and 16 SMRT cells. The PHQIs were generated using IsoSeqTM (SMRT® Portal version 2.2.0) Structure/ processing Classification No. (isoforms) 8 cells 16 Consensus calling (ICE) No. consensus isoforms 63,737 113,316 Average length of consensus isoforms 2,610 2,645 Quality Filtering (Quiver) No. polished high-quality isoforms 5,786 10,449 No. polished low-quality isoforms 5,989 8,800 Table S5. Production of PHQIs and average length from each tissue: flower, leaf, bulb and root. Due to overlapped gene expression among the tissues, total reads from the four tissues were combined to
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