Pus / Sterile fluids Clinical / Gram Standard media Incubation Cultures read Target organism(s) strain Temp (°C) Atmosphere Time All Blood agar 35 37 5 10% CO2 40 - 48h Daily streptococci – – β-haemolytic spp. (bite wounds) Pasteurella S. aureus Vibrio spp. Chocolate agar 35 37 5 10% CO2 40 - 48h Daily Haemophilus spp. – – MacConkey agar 35 37 Air 40 - 48h Daily Enterobacteriaceae – Pseudomonads Sabouraud agar 35 37 Air 40 - 48h Daily Fungi – If site CNA-blood agar 35 37 Air 40 - 48h Daily streptococci “non-sterile” – β-haemolytic S. aureus If ?melioid or ASH 35 37 Air 48h (Sub Daily B. pseudomallei – SB to ASH parotitis SB at 48h)
Pus / Sterile fluids Clinical / Gram strain Standard media Incubation Cultures read Target organism(s) Temp (°C) Atmosphere Time All Blood agar 35 – 37 5 – 10% CO2 40 - 48h Daily β-haemolytic streptococci Pasteurella spp. (bite wounds) S. aureus Vibrio spp. Chocolate agar 35 – 37 5 – 10% CO2 40 - 48h Daily Haemophilus spp. MacConkey agar 35 – 37 Air 40 - 48h Daily Enterobacteriaceae Pseudomonads Sabouraud agar 35 – 37 Air 40 - 48h Daily Fungi If “non-sterile” site CNA-blood agar 35 – 37 Air 40 - 48h Daily β-haemolytic streptococci S. aureus If ?melioid or parotitis ASH SB 35 – 37 Air 48h (Sub SB to ASH at 48h) Daily B. pseudomallei
Culture_of_Pus_Swabs.docx