listed in Table 2. The frequency of remaining granular cells was indicated as percentage of the total number of pixel and classed into four degrees, that is, high remaining level; over or around 50% of cell was stained by hematoxylin, middle remaining level; from 30 to 40% of the cells were stained, low remaining level; around 20% of cells were stained, and severe degeneration level; around 10% of cells were remained with large amount of cell debris (synaptic glomeruli). Case 1,2,3,7,and 8 were grouped into high remaining case 4,11 and 14 were in moderate group, case 5, 9, 10 and 12 were in low group and case 6 and 13 were grouped into severe level. As an interesting case, the granular cell layer of the cerebellum obtained from case 12 individual whose postmortem interval was presumed over 40 days, decreased the stain-ability, however the morphology of the granular cells have been well keeping compared with the longer agonal duration cases that was classed into severe level. Changing of granular cell was remarkable compared with that of Purkinje cells in lower and severe level groups where the morphology of Purkinje cells was still remained. [2] Immunohistochemical staining In the granular cell layer at high level remaining, a large amount of granular cell nucleus showed good and intensive reactivity with anti CIRBP and RBM3 antibodies. HSP70 showed weak or no reactivity with the nucleus of
listed in Table 2. The frequency of remaining granular cells was indicated as percentage of the total number of pixel and classed into four degrees, that is, high remaining level; over or around 50% of cell was stained by hematoxylin, middle remaining level; from 30 to 40% of the cells were stained, low remaining level; around 20% of cells were stained, and severe degeneration level; around 10% of cells were remained with large amount of cell debris (synaptic glomeruli). Case 1,2,3,7,and 8 were grouped into high remaining case 4,11 and 14 were in moderate group, case 5, 9, 10 and 12 were in low group and case 6 and 13 were grouped into severe level. As an interesting case, the granular cell layer of the cerebellum obtained from case 12 individual whose postmortem interval was presumed over 40 days, decreased the stain-ability, however the morphology of the granular cells have been well keeping compared with the longer agonal duration cases that was classed into severe level. Changing of granular cell was remarkable compared with that of Purkinje cells in lower and severe level groups where the morphology of Purkinje cells was still remained. [2] Immunohistochemical staining In the granular cell layer at high level remaining, a large amount of granular cell nucleus showed good and intensive reactivity with anti CIRBP and RBM3 antibodies. HSP70 showed weak or no reactivity with the nucleus of
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