You will draw out the steps of the S-phase of Interphase, DNA replication. In each box, draw the event described. You will use 3 different colors: one for the original strands of DNA, one for the leading strand, and one for the lagging strand. You must label all the bold words below in each drawing. Use the following link to help you, using the Fork” and with Proteins” links at the top of the page to “Replication “Fork help you: . 1. Draw the DNA 2. Draw helicase as it 3. DNA 4. Primase adds a 5. Two DNA double double helix, with begins to unwind the polymerase III short RNA segment to helices are formed, this sequence on the DNA at the adds complementary the lagging stand. showing 5’ to 3’ strand: replication fork. bases continuously DNA polymerase ACCGTATTGATC Be sure to include the in the 5’ to 3’ III adds replication. Label the 5’ and 3’ on single-stranding direction (leading complementary bases each strand. binding proteins strand). discontinuously in the 5’ to 3’ direction, (SSBPs). forming Okazaki fragments. RNA primer replaced with DNA by DNA polymerase I; and finally, DNA fragments are connected by DNA ligase. Key terms to know for this set of lessons on DNA replication: Helicase Okazaki fragment semi-conservative replication Topioisomerase DNA ligase replication fork Single-stranded binding proteins leading strand parent DNA DNA polymerase I (DNA pol I) lagging strand enzyme DNA polymerase III (DNA pol III) continuous replication DNA primase discontinuous replication
You will draw out the steps of the S-phase of Interphase, DNA replication. In each box, draw the event described. You will use 3 different colors: one for the original strands of DNA, one for the leading strand, and one for the lagging strand. You must label all the bold words below in each drawing. Use the following link to help you, using the “Replication Fork” and “Fork with Proteins” links at the top of the page to help you: . 1. Draw the DNA double helix, with this sequence on the 5’ to 3’ strand: ACCGTATTGATC Label the 5’ and 3’ on each strand. 2. Draw helicase as it begins to unwind the DNA at the replication fork. Be sure to include the single-stranding binding proteins (SSBPs). 3. DNA polymerase III adds complementary bases continuously in the 5’ to 3’ direction (leading strand). 4. Primase adds a short RNA segment to the lagging stand. DNA polymerase III adds complementary bases discontinuously in the 5’ to 3’ direction, forming Okazaki fragments. RNA primer replaced with DNA by DNA polymerase I; and finally, DNA fragments are connected by DNA ligase. 5. Two DNA double helices are formed, showing replication. Key terms to know for this set of lessons on DNA replication: Helicase Okazaki fragment semi-conservative replication Topioisomerase DNA ligase replication fork Single-stranded binding proteins leading strand parent DNA DNA polymerase I (DNA pol I) lagging strand enzyme DNA polymerase III (DNA pol III) continuous replication DNA primase discontinuous replication
dna_replication_worksheet.docx