Day 1 - Monday 19th September 2016 hrs Arrival and registration hrs Introductory lecture, including coffee and discussion 10:00 11:00 hrs Tour of the Mary Lyon Centre – 11:00 12:00 hrs Handling/Shipping Samples Introduction – Lifor holding/refrigerated transportation Dry shipper (Demonstration) 12:00 hrs –13:00 13:00 15:00 hrs Sperm Harvesting/Freezing Introduction – freezing - Practical held in Lifor for refrigerated transportation (Practical) Sperm held on dry-ice (Demonstration) 15:00 16:00 hrs Preparation for Tuesday’s IVF – Pre- incubation dish containing MBCD Fertilisation drops containing GSH Wash dish containing mHTF 16:00 16:30 hrs Recap/Review – Day 2 - Tuesday 20th September 2016 07:30 - 10:30 hrs IVF Introduction Freshly harvested sperm Demonstration – Sperm retained in epididymides (Lifor) Practical – Harvest oocytes Demonstration followed by – practical Sperm frozen in LN2 (Practical) Sperm held on dry-ice (Demonstration) hrs TEA AND COFFEE 11:00 12:30 hrs Embryo handling (Practical) –
Day 1 - Monday 19th September 2016 hrs Arrival and registration hrs Introductory lecture, including coffee and discussion 10:00 – 11:00 hrs Tour of the Mary Lyon Centre 11:00 – 12:00 hrs Handling/Shipping Samples Introduction Lifor holding/refrigerated transportation Dry shipper (Demonstration) 12:00 –13:00 hrs 13:00 – 15:00 hrs Sperm Harvesting/Freezing Introduction freezing - Practical held in Lifor for refrigerated transportation (Practical) Sperm held on dry-ice (Demonstration) 15:00 – 16:00 hrs Preparation for Tuesday’s IVF Pre- incubation dish containing MBCD Fertilisation drops containing GSH Wash dish containing mHTF 16:00 – 16:30 hrs Recap/Review Day 2 - Tuesday 20th September 2016 07:30 - 10:30 hrs IVF Introduction Freshly harvested sperm – Demonstration Sperm retained in epididymides (Lifor) – Practical Harvest oocytes – Demonstration followed by practical Sperm frozen in LN2 (Practical) Sperm held on dry-ice (Demonstration) hrs TEA AND COFFEE 11:00 – 12:30 hrs Embryo handling (Practical)
cryocourse_agenda_-_sept_2016.docx