Supplementary Figure S5 Supplementary Figure S5. The time course changes of active capase-3 in astrocytes. (a) Astrocytes were suffered OGD treatment for 1h, 3h, 6h and 12h, and the double immunofluorescence staining of caspase-3 (green) and GFAP (red) was performed by corresponding antibodies. DAPI (blue) was used to stain nuclei. Images were captured by the confocal microscopy. Magnified images (M) were cropped sections from the merge images (white borders). Magnification ×200. (b) Quantification of active capase-3-positive cells as a percentage of total
Supplementary Figure S5 Supplementary Figure S5. The time course changes of active capase-3 in astrocytes. (a) Astrocytes were suffered OGD treatment for 1h, 3h, 6h and 12h, and the double immunofluorescence staining of caspase-3 (green) and GFAP (red) was performed by corresponding antibodies. DAPI (blue) was used to stain nuclei. Images were captured by the confocal microscopy. Magnified images (M) were cropped sections from the merge images (white borders). Magnification ×200. (b) Quantification of active capase-3-positive cells as a percentage of total
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