Time: 3 hrs Objectives: The purpose of the course is to teach the students about basics and advanced ensuring that students acquire an extensive and sound concepts of Molecular Genetics and knowledge for future studies. After the completion of the course, the students would acquire the knowledge of Outcomes: genome structure and organization in eukaryotes, DNA mutability, transcription regulation in prokaryotes and eukaryotes, site specific recombination and its biological role, transposition and its mechanisms. They would learn advanced techniques of genome mapping and sequencing, comparative genomics and transcriptome analysis. They would also be acquainted with methodological concepts and tools needed to acquire top-level skills in the field of molecular genetics NOTE: Nine questions will be set in all Question No. 1, which will be short answer type covering the entire syllabus, will be compulsory. The remaining eight questions will be set unit-wise with two questions from each unit. The candidates will be required to attempt Question No. 1 and four others selecting one question from each unit. All questions will carry equal marks. Unit I – Eukaryotic Genome Structure and Organization Genome sequence and chromosome diversity, variation in chromosome number, Special features of metaphase chromosomes, Chromosome banding, Genome size and complexity, organization and content of human genome, Repetitive DNA, Microsatellites, genome wide repeats, Split genes, overlapping genes, cryptic genes, Retrogenes, Multigene families, Pseudo genes Nucleosome-Basic Structure, spatial arrangements of histones, chromatosome, Solenoid model, Chromatin domains, Chromatin modifications The Mutability of DNA An overview of mutation and polymorphism, VNTR polymorphism, DNA damage- spontaneous, Induced (Alkylation, oxidation, radiation), Genotoxicity/ mutagenicity test systems - Ames test, Sister chromatid exchanges, Micronucleus, Comet assay Unit II – Transcription Regulation in Prokaryotes
Time: 3 hrs Objectives: The purpose of the course is to teach the students about basics and advanced concepts of Molecular Genetics and ensuring that students acquire an extensive and sound knowledge for future studies. Outcomes: After the completion of the course, the students would acquire the knowledge of genome structure and organization in eukaryotes, DNA mutability, transcription regulation in prokaryotes and eukaryotes, site specific recombination and its biological role, transposition and its mechanisms. They would learn advanced techniques of genome mapping and sequencing, comparative genomics and transcriptome analysis. They would also be acquainted with methodological concepts and tools needed to acquire top-level skills in the field of molecular genetics NOTE: Nine questions will be set in all Question No. 1, which will be short answer type covering the entire syllabus, will be compulsory. The remaining eight questions will be set unit-wise with two questions from each unit. The candidates will be required to attempt Question No. 1 and four others selecting one question from each unit. All questions will carry equal marks. Unit – I Eukaryotic Genome Structure and Organization Genome sequence and chromosome diversity, variation in chromosome number, Special features of metaphase chromosomes, Chromosome banding, Genome size and complexity, organization and content of human genome, Repetitive DNA, Microsatellites, genome wide repeats, Split genes, overlapping genes, cryptic genes, Retrogenes, Multigene families, Pseudo genes Nucleosome-Basic Structure, spatial arrangements of histones, chromatosome, Solenoid model, Chromatin domains, Chromatin modifications The Mutability of DNA An overview of mutation and polymorphism, VNTR polymorphism, DNA damage- spontaneous, Induced (Alkylation, oxidation, radiation), Genotoxicity/ mutagenicity test systems - Ames test, Sister chromatid exchanges, Micronucleus, Comet assay Unit – II Transcription Regulation in Prokaryotes
CBCS%20Msc%20Biotechnology%202016-17%20latest%20RECTIFIED%208_9_2017.docx