Supplementary Table 1. Antibody used for immunoblotting MARKER (SPECIES) DILUTION DISTRIBUTOR/SOURCE (CATALOG NUMBER) Primary antibody: WB: (H300) rabbit IgG 1:1000 Cruz (sc-11414) (C-19) goat IgG 1:1000 Santa Cruz (sc-13298) Drp-1 (H-300) rabbit IgG 1:1000 (sc-32898) Phospho-Drp1 (Ser616) (D9A1) rabbit mAb 1:1000 CST (4494) Phospho-Drp1 (Ser637) (D3A4) rabbit mAb 1:1000 CST (6319) GAPDH (14C10) rabbit Ab 1:2000 CST (sc-659) MFN1 rabbit mAb 1:1000 CST (14739) MFN2 (D1E9) rabbit mAb 1:1000 CST (11925) OPA1 antibody (D-9) 1:1000 Santa Cruz (sc-393296) ERK 1/2 antibody 1:1000 CST (9102) p-ERK (Thr202/Tyr204) antibody 1:1000 CST (9101s) Akt (C67E7) rabbit mAb 1:1000 CST (4691s) p-Akt rabbit mAb 1:1000 CST (4046s) p38MAPK rabbit mAb 1:1000 CST (9212) phopho-p38MAPK (Thr180/Tyr182) Antibody 1:1000 CST (9211) Secondary antibody: WB: 1:5000 CST (7076) IgG HRP-linked Ab 1:5000 CST (7074) Anti-rabbit IgG HRP-linked Ab 1:5000 Santa Cruz (sc-2020) Donkey anti-goat IgG HRP Ab
MARKER (SPECIES) DILUTION DISTRIBUTOR/SOURCE (CATALOG NUMBER) Primary antibody: WB: (H300) rabbit IgG (C-19) goat IgG 1:1000 1:1000 Cruz (sc-11414) Santa Cruz (sc-13298) Drp-1 (H-300) rabbit IgG Phospho-Drp1 (Ser616) (D9A1) rabbit mAb Phospho-Drp1 (Ser637) (D3A4) rabbit mAb 1:1000 1:1000 1:1000 (sc-32898) CST (4494) CST (6319) GAPDH (14C10) rabbit Ab MFN1 rabbit mAb MFN2 (D1E9) rabbit mAb OPA1 antibody (D-9) ERK 1/2 antibody p-ERK (Thr202/Tyr204) antibody Akt (C67E7) rabbit mAb p-Akt rabbit mAb p38MAPK rabbit mAb phopho-p38MAPK (Thr180/Tyr182) Antibody 1:2000 1:1000 1:1000 1:1000 1:1000 1:1000 1:1000 1:1000 1:1000 1:1000 CST (sc-659) CST (14739) CST (11925) Santa Cruz (sc-393296) CST (9102) CST (9101s) CST (4691s) CST (4046s) CST (9212) CST (9211) Secondary antibody: WB: IgG HRP-linked Ab Anti-rabbit IgG HRP-linked Ab Donkey anti-goat IgG HRP Ab 1:5000 1:5000 1:5000 CST (7076) CST (7074) Santa Cruz (sc-2020) Supplementary Table 1. Antibody used for immunoblotting
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