-Uses filter to isolate a narrow wavelength of the spectrum for measurements Define Spectrophotometry -measurement of intensity of light at selected wavelengths -Use prisms or gratings to isolate a narrow wavelength range of the spectrum for measurements What is Photometric The process use to measure intensity independent of measurement ? wavelength What is wavelength? A characteristic of electromagnetic radiation; the distance between two wave crests that is measured in nanometers (nm) What does Beer’s Law state? The concentration of a substance is directly proportional to the amount of absorbed or is inversely proportional to the logarithm of the transmitted light. What is a tungsten light bulb? A light source in the visible range of spectra. An example is a quartz halogen lamp. What is the function of a Supplies the needed energy in the UV region. It is used for mercury vapor lamp calibration purposes but is not practical for absorbance measurements because it is used only at certain wavelengths Name the 10 parts that are in a Manual operation procedure Single-beam Types of light sources spectrophotometer. Filters Monochromators Fiber optics Cuvets Photodetectors Readout devices Digital hardware and software Recorders Name the 2 types of Light Incandescent and source. Name the 3 types of Filters. Prisms, Gratings Spectrophotometers are Single- or Double-beam classified as a What are the 2 choices of Bandwidth and Turbidity monochromators. What does a cuvet do? Absorbs a portion of radiant energy. What does a photodetector do? Converts light into an electrical signal that is proportional to the number of photons. Name the 4 Performance Comparison of unknown with calibrator(s) parameters of an instrument. Comparison with published/previously determined values NIST-provided SRMS for comparison IRMM-provided reference materials What does NIST for? National Institute if Standards and Technology What does SRMS stand for? Standard Reference Materials What does IRMM for? Institute for Reference Material and Measurements
-Uses filter to isolate a narrow wavelength of the spectrum for measurements Define Spectrophotometry -measurement of intensity of light at selected wavelengths -Use prisms or gratings to isolate a narrow wavelength range of the spectrum for measurements What is Photometric measurement ? The process use to measure intensity independent of wavelength What is wavelength? A characteristic of electromagnetic radiation; the distance between two wave crests that is measured in nanometers (nm) What does Beer’s Law state? The concentration of a substance is directly proportional to the amount of absorbed or is inversely proportional to the logarithm of the transmitted light. What is a tungsten light bulb? A light source in the visible range of spectra. An example is a quartz halogen lamp. What is the function of a mercury vapor lamp Supplies the needed energy in the UV region. It is used for calibration purposes but is not practical for absorbance measurements because it is used only at certain wavelengths Name the 10 parts that are in a Single-beam spectrophotometer. Manual operation procedure Types of light sources Filters Monochromators Fiber optics Cuvets Photodetectors Readout devices Digital hardware and software Recorders Name the 2 types of Light source. Incandescent and Name the 3 types of Filters. Prisms, Gratings Spectrophotometers are classified as a Single- or Double-beam What are the 2 choices of monochromators. Bandwidth and Turbidity What does a cuvet do? Absorbs a portion of radiant energy. What does a photodetector do? Converts light into an electrical signal that is proportional to the number of photons. Name the 4 Performance parameters of an instrument. Comparison of unknown with calibrator(s) Comparison with published/previously determined values NIST-provided SRMS for comparison IRMM-provided reference materials What does NIST for? National Institute if Standards and Technology What does SRMS stand for? Standard Reference Materials What does IRMM for? Institute for Reference Material and Measurements
ChemPPT%20Flashcards%20Unit%201.docx