From the temperature data, what can you conclude about how temperature affects enzyme activity? How would you explain the results? What is meant by optimum temperature? According to the class data, what is the optimum data for the potato catalase and why? What would you predict if you had first mixed the catalase (100%) in serial solutions of varying pHs of 3, 5, 7, 9, 11? What would you predict would be optimum pH? Design a controlled experiment to test the effect of varying pH, temperature, or enzyme concentration. DATA TABLES Name _________________________________ Date _______________ Lab partner(s) ___________________________ Table 1 Effect of Enzyme Concentration on Rate of Activity – Enzyme Enzyme Concentration Concentration Time to Float Disc (in seconds) % Rate Class Average (units/mL) Trial 1 2 Average 100 100 75 75 50 50 25 25 0 0 Table 2 Effect of Substrate Concentration on Enzyme Activity – Substrate Concentration Time to Float Disc (in seconds) Rate Class (from serial Average dilutions) Trial 1 2 Average 0.0 % H2O2 0.75% H2O2
From the temperature data, what can you conclude about how temperature affects enzyme activity? How would you explain the results? What is meant by optimum temperature? According to the class data, what is the optimum data for the potato catalase and why? What would you predict if you had first mixed the catalase (100%) in serial solutions of varying pHs of 3, 5, 7, 9, 11? What would you predict would be optimum pH? Design a controlled experiment to test the effect of varying pH, temperature, or enzyme concentration. DATA TABLES Name _________________________________ Date _______________ Lab partner(s) ___________________________ Table 1 – Effect of Enzyme Concentration on Rate of Activity Enzyme Concentration Enzyme Concentration Time to Float Disc (in seconds) % (units/mL) Trial 1 2 Average Rate Class Average 100 100 75 75 50 50 25 25 0 0 Table 2 – Effect of Substrate Concentration on Enzyme Activity Substrate Concentration Time to Float Disc (in seconds) (from serial dilutions) Trial 1 2 Average Rate Class Average 0.0 % H2O2 0.75% H2O2
Catalase_Lab_Adapted_for_Biology_I.316125830.docx