mediator pathways on bladder function under control conditions, and secondly to ensure complete block of the various signalling and receptor pathways prior to high K+ solution stimulation. Intraluminal perfusion of the cocktail in saline had no effect on mechanosensitivity. Baseline afferent nerve firing was decreased following perfusion of the cocktail in saline, as was bladder compliance (figure 6.10). Intraluminal perfusion of the cocktail in saline had no effect on afferent firing in response to ramp distension of the bladder. Afferent firing in response to bladder distension remained stable for 3 consecutive distensions prior to intraluminal perfusion of the cocktail in saline solution. Following 30 minutes continuous intraluminal perfusion of the bladder with the cocktail in saline, afferent nerve firing in response to ramp distension (to 50mmHg IP) was unaffected, relative to control distension (figure 6.11A). Similarly, following 60 minutes continuous intraluminal of the cocktail in saline, afferent nerve firing remained unchanged relative to control (figure 6.11B). Bladder compliance was decreased, relative to control, following intraluminal perfusion of the cocktail in saline. Bladder compliance remained stable for 3 consecutive distensions prior to continuous intraluminal perfusion of the cocktail in saline solution. Following 30 minutes intraluminal perfusion of the bladder with the cocktail in saline, bladder compliance was decreased by 11% relative to control compliance, requiring a smaller volume of perfusion solution to distend the bladder to 50mmHg IP than control (figure 6.12A). Bladder compliance was further decreased (15%), relative to control, following 60 minutes intraluminal perfusion of the cocktail in saline (figure 6.12B).
mediator pathways on bladder function under control conditions, and secondly to ensure complete block of the various signalling and receptor pathways prior to high K+ solution stimulation. Intraluminal perfusion of the cocktail in saline had no effect on mechanosensitivity. Baseline afferent nerve firing was decreased following perfusion of the cocktail in saline, as was bladder compliance (figure 6.10). Intraluminal perfusion of the cocktail in saline had no effect on afferent firing in response to ramp distension of the bladder. Afferent firing in response to bladder distension remained stable for 3 consecutive distensions prior to intraluminal perfusion of the cocktail in saline solution. Following 30 minutes continuous intraluminal perfusion of the bladder with the cocktail in saline, afferent nerve firing in response to ramp distension (to 50mmHg IP) was unaffected, relative to control distension (figure 6.11A). Similarly, following 60 minutes continuous intraluminal of the cocktail in saline, afferent nerve firing remained unchanged relative to control (figure 6.11B). Bladder compliance was decreased, relative to control, following intraluminal perfusion of the cocktail in saline. Bladder compliance remained stable for 3 consecutive distensions prior to continuous intraluminal perfusion of the cocktail in saline solution. Following 30 minutes intraluminal perfusion of the bladder with the cocktail in saline, bladder compliance was decreased by 11% relative to control compliance, requiring a smaller volume of perfusion solution to distend the bladder to 50mmHg IP than control (figure 6.12A). Bladder compliance was further decreased (15%), relative to control, following 60 minutes intraluminal perfusion of the cocktail in saline (figure 6.12B).
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