If the specimen contains only a small clot, this should be included either in the enrichment culture or inoculated to the chocolate agar plate. The unclotted portion of the specimen should be cultured in the normal way as described above. 2.5.4 Supplementary If blood culture bottles are used, inoculate bottles with the un centrifuged specimen. If culture negative result from clinically ill patient consider other non-culture methods for identification for a result eg 16S PCR, Maldi TOFF, etc 2.5.5 Culture media, conditions and organisms Clinical Specimen Standard Incubation Cultures Target organism(s) details/ media read Temp Time conditions °C Infection Amniotic Blood agar 35-37 5-10% 40-48hr daily Any organism suspected fluid, bursa CO2 in a fluid, Fastidious 35-37 Anaerobes normally pericardial ≥48hr anaerobe agar 48hr* sterile site fluid, synovial (joint) fluid, Chocolate 35-37 5-10% 40-48hr daily Any organism peritoneal agar fluid CO2 (ascites), pleural fluid Either: Supplemented 35-37 air N/A Any organism blood culture bottles† or or 35-37 air N/A Any organism 40-48hr Anaerobic 35-37 as daily 40-48hr Subcultured at above 40hr on to the above media as appropriate to clinical details For these situations, add the following: Clinical Specimen Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C Peritonitis Neomycin Ascitic 35-37 40 Anaerobes – ≥48hr fastidious fluid anaerobe agar
If the specimen contains only a small clot, this should be included either in the enrichment culture or inoculated to the chocolate agar plate. The unclotted portion of the specimen should be cultured in the normal way as described above. 2.5.4 Supplementary If blood culture bottles are used, inoculate bottles with the un centrifuged specimen. If culture negative result from clinically ill patient consider other non-culture methods for identification for a result eg 16S PCR, Maldi TOFF, etc 2.5.5 Culture media, conditions and organisms Clinical details/ conditions Specimen Standard media Incubation Cultures read Target organism(s) Temp °C Time Infection suspected in a normally sterile site Amniotic fluid, bursa fluid, pericardial fluid, synovial (joint) fluid, peritoneal fluid (ascites), pleural fluid Blood agar 35-37 5-10% CO2 40-48hr daily Any organism Fastidious anaerobe agar 35-37 ≥48hr Anaerobes Chocolate agar 35-37 5-10% CO2 40-48hr daily Any organism Either: Supplemented blood culture bottles† or Anaerobic Subcultured at 40hr on to the above media as appropriate to clinical details 35-37 35-37 35-37 air air as above or 40-48hr 40-48hr N/A N/A daily Any organism Any organism For these situations, add the following: Clinical details/ conditions Specimen media Incubation Cultures read Target organism(s) Temp °C Atmos Time Peritonitis Ascitic fluid Neomycin fastidious anaerobe agar 35-37 40 – 48hr* ≥48hr Anaerobes
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