Biological specimens (Gastric lavage, Viscera and Blood) which creates hindrances in 15 identification of target drug. S.P.E. has been used as an effective, environmental friendly, 16 economical and proficient technique in separating drug from forensic biological specimens with 17 for high quality of recovery. A comparison is carried out between S.P.E. and conventional 18 L.L.E. for extraction of drug from biological matrices showed that S.P.E. is the better 19 technique for sample extraction and preparation of various biological specimens with trace level 20 of of analytes. 21 Keywords: Solid phase extraction (S.P.E.), Liquid-Liquid Extraction (L.L.E.), Cephalosporin 22 and Carbapenem. 23 and 24 INTRODUCTION 25 in The aim of forensic toxicological study is to isolate and clean up different antibiotic drugs in 26 various matrices of biological or non-biological origin to yield desired amount to which 27 spectrophotometric, chromatographic and other instrumental techniques can be applied 28 successfully for determination of analytes. In forensic sciences, sample clean-up process is 29 14 necessary for eliminating coagulated proteins and large amount of fat molecules for 30 inhibiting the interference with chemical activity of target drug present in matrix. For this it is 31 necessary to simple, precise, economical cleanup process. Since extraction is primary step 32 The for chemical analysis, it should be applied in a way that it give high quality yield of 33 analytes in forensic toxicological specimens.Thus Solid Phase Extraction technique is used 34 up to extract analyte in trace levels in large number of samples with easy automation which 35 is not available in conventional extraction techniques such as Liquid-Liquid 36
for of and in 14 The 15 Biological specimens (Gastric lavage, Viscera and Blood) which creates hindrances in 16 identification of target drug. S.P.E. has been used as an effective, environmental friendly, 17 economical and proficient technique in separating drug from forensic biological specimens with 18 high quality of recovery. A comparison is carried out between S.P.E. and conventional 19 L.L.E. for extraction of drug from biological matrices showed that S.P.E. is the better 20 technique for sample extraction and preparation of various biological specimens with trace level 21 of analytes. 22 Keywords: Solid phase extraction (S.P.E.), Liquid-Liquid Extraction (L.L.E.), Cephalosporin 23 and Carbapenem. 24 25 INTRODUCTION 26 The aim of forensic toxicological study is to isolate and clean up different antibiotic drugs in 27 various matrices of biological or non-biological origin to yield desired amount to which 28 spectrophotometric, chromatographic and other instrumental techniques can be applied 29 successfully for determination of analytes. In forensic sciences, sample clean-up process is 30 necessary for eliminating coagulated proteins and large amount of fat molecules for 31 inhibiting the interference with chemical activity of target drug present in matrix. For this it is 32 necessary to simple, precise, economical cleanup process. Since extraction is primary step 33 for chemical analysis, it should be applied in a way that it give high quality yield of 34 analytes in forensic toxicological specimens.Thus Solid Phase Extraction technique is used 35 to extract analyte in trace levels in large number of samples with easy automation which 36 is not available in conventional extraction techniques such as Liquid-Liquid up
Comparative%20Study%20for%20Isolation.docx