Procedures with a high potential for creating aerosols are conducted. These may include centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption, opening containers of materials whose internal pressures may be different from ambient pressures High concentrations or large volumes of organisms containing recombinant DNA molecules are used. Such materials may be centrifuged in the open laboratory if sealed beads or centrifuge safety cups are used and if they are opened only in a biological safety cabinet. A properly maintained biological safety cabinet (Class I or II), will have a current, annual certification that under normal operating circumstances the unit performs to manufacturer’s specification. Face protection (goggles, mask, face shield or splatter guard) is used for anticipated splashes or sprays of infectious or other hazardous materials to the face when the microorganisms must be manipulated outside the biological safety cabinet. BL2 Laboratory Facilities All BL1 Laboratory Facility Requirements, AND Provide lockable doors for facilities that house restricted agents. Install biological safety cabinets in such a manner that fluctuations of the room supply and exhaust air do not cause the biological safety cabinets to operate outside their parameters for containment. Locate biological safety cabinets away from doors, from windows that can be opened, from heavily traveled laboratory areas, and from other potentially disruptive equipment so as to maintain the biological safety cabinets’ air flow parameters for containment. An eyewash station is readily available. Illumination is adequate for all activities, avoiding reflections and glare that could impede vision. An autoclave for decontaminating laboratory wastes is available. Biological Disposal Potentially hazardous biological/physical will be removed (from appropriately labeled containers located in biology department laboratories and appropriately packaged for disposal by JMU biology personnel specially trained in handling of these wastes. Autoclave Validation
Procedures with a high potential for creating aerosols are conducted. These may include centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption, opening containers of materials whose internal pressures may be different from ambient pressures High concentrations or large volumes of organisms containing recombinant DNA molecules are used. Such materials may be centrifuged in the open laboratory if sealed beads or centrifuge safety cups are used and if they are opened only in a biological safety cabinet. A properly maintained biological safety cabinet (Class I or II), will have a current, annual certification that under normal operating circumstances the unit performs to manufacturer’s specification. Face protection (goggles, mask, face shield or splatter guard) is used for anticipated splashes or sprays of infectious or other hazardous materials to the face when the microorganisms must be manipulated outside the biological safety cabinet. BL2 Laboratory Facilities All BL1 Laboratory Facility Requirements, AND Provide lockable doors for facilities that house restricted agents. Install biological safety cabinets in such a manner that fluctuations of the room supply and exhaust air do not cause the biological safety cabinets to operate outside their parameters for containment. Locate biological safety cabinets away from doors, from windows that can be opened, from heavily traveled laboratory areas, and from other potentially disruptive equipment so as to maintain the biological safety cabinets’ air flow parameters for containment. An eyewash station is readily available. Illumination is adequate for all activities, avoiding reflections and glare that could impede vision. An autoclave for decontaminating laboratory wastes is available. Biological Disposal Potentially hazardous biological/physical will be removed (from appropriately labeled containers located in biology department laboratories and appropriately packaged for disposal by JMU biology personnel specially trained in handling of these wastes. Autoclave Validation
Chemical%20Hygiene%20Plan%20Apr18.docx