Practice using dissecting scope to differentiate contamination in struck hits Record ideas and experimental procedure in Lab notebook Sept 27 Oct 2 1) Re-streak all hits two more times onto fresh – plates; use dissecting scope to be sure purifying Oct 3 Midterm Prepare for midterm 1) Make frozen stocks of hits from 1st round of co-culturing Oct 9 Streak out possible hits from freezer Oct 10 1) Discuss possible additional reporters Read 16S identification and TA will summarize phenotypes bacterial phylogeny Discuss possible additional treatments? Get new reporters and set up co-culture Work on lab report from Sept screen 26 experiment Set up PCR of existing hits Set up secondary screen of hits from 1st round TA will put PCR reactions into 4 degrees either this evening or tomorrow morning Oct 11 Come in to record results of secondary screen Oct 16 1) Plate new co-cultures with new reporters Oct 17 1) Turn in Lab Report Read about BLAST (link on Kit purify PCR reactions (only those that Sakai) passed secondary screen) Run DNA gel of PCR reactions (Quantify?) Send off DNA for sequencing (only those that have sufficient DNA) Screen co-culture plates with new reporters/conditions and pick and streak additional hits Oct 18 Oct 23 1) Re-streak all hits two more times onto fresh – plates; use dissecting scope to be sure purifying different colony types Oct 23 Set up secondary screen of new hits from additional reporter co-cultures Streak out hits from first round Oct 24 1) Examine secondary screen from new hits Need laptops for DNA analysis 2) Do PCR of new hits
Practice using dissecting scope to differentiate contamination in struck hits Record ideas and experimental procedure in Lab notebook Sept 27 – Oct 2 1) Re-streak all hits two more times onto fresh plates; use dissecting scope to be sure purifying Oct 3 Midterm 1) Make frozen stocks of hits from 1st round of co-culturing Prepare for midterm Oct 9 Streak out possible hits from freezer Oct 10 1) Discuss possible additional reporters TA will summarize phenotypes Discuss possible additional treatments? Get new reporters and set up co-culture screen Set up PCR of existing hits Set up secondary screen of hits from 1st round TA will put PCR reactions into 4 degrees either this evening or tomorrow morning Read 16S identification and bacterial phylogeny Work on lab report from Sept 26 experiment Oct 11 Come in to record results of secondary screen Oct 16 1) Plate new co-cultures with new reporters Oct 17 1) Turn in Lab Report Kit purify PCR reactions (only those that passed secondary screen) Run DNA gel of PCR reactions (Quantify?) Send off DNA for sequencing (only those that have sufficient DNA) Screen co-culture plates with new reporters/conditions and pick and streak additional hits Read about BLAST (link on Sakai) Oct 18 – Oct 23 1) Re-streak all hits two more times onto fresh plates; use dissecting scope to be sure purifying different colony types Oct 23 Set up secondary screen of new hits from additional reporter co-cultures Streak out hits from first round Oct 24 1) Examine secondary screen from new hits 2) Do PCR of new hits Need laptops for DNA analysis
102-lab-Stegenga-F18-TENTATIVE-1.docx