What is first order kinetics? in an enzymatic reaction, as more substrate is consumed, the reaction rate declines and becomes dependent on substrate concentration; this is a phrase of first-order dependence on substrate concentration when the rate of the reaction is proportional to the concentration of substrate or when the enzyme concentration is fixed and the substrate concentration is varied. What is kinetics? In an enzymatic reaction, when the reaction rate is constant at maximum value, the reaction rate depends only on enzyme concentration and is independent of substrate concentration; the rate of reaction is proportional to the zero power of the substrate concentration. What is a constant for a given enzyme acting Michaelis-Menten constant (Km) under given conditions; the experimentally determined substrate concentration at which the enzymatic reaction velocity equals ½ of the maximum velocity of the enzymatic reaction? What is a plot of the reciprocal of the velocity Lineweaver-Burk plot of an enzyme catalyzed reaction (ordinate; against the reciprocal of the substrate concentration (abscissa; x-axis)? What shows maximum activity in vitro in the pH from 7 to 8 pH range of enzymes in plasma? What are the activity values observed in pH as Low as 1.5 (pepsin) low and high? High as 10.5 (ALP) What can be controlled by the use of buffer Pronounced effects of pH on enzyme reactions. solutions? When does enzymes inactivation and Occurs at temperature of 60 degrees Celsius to denaturation occurs? 70 degrees Celsius. What is the accepted measuring temperature 37 degrees Celsius for enzymes in the clinical laboratory? What is a substance that reduces the rate of an Inhibitor enzymatic reaction and is classified as reversible or irreversible? What is a structural analog of the substrate that Competitive inhibitor combines with the free enzyme and competes with the normal substrate for binding at the active site? What is structurally different from the Noncompetitive inhibitor substrate, it binds at a site on the enzyme molecule that is different from the site?
What is first order kinetics? in an enzymatic reaction, as more substrate is consumed, the reaction rate declines and becomes dependent on substrate concentration; this is a phrase of first-order dependence on substrate concentration when the rate of the reaction is proportional to the concentration of substrate or when the enzyme concentration is fixed and the substrate concentration is varied. What is kinetics? In an enzymatic reaction, when the reaction rate is constant at maximum value, the reaction rate depends only on enzyme concentration and is independent of substrate concentration; the rate of reaction is proportional to the zero power of the substrate concentration. What is a constant for a given enzyme acting under given conditions; the experimentally determined substrate concentration at which the enzymatic reaction velocity equals ½ of the maximum velocity of the enzymatic reaction? Michaelis-Menten constant (Km) What is a plot of the reciprocal of the velocity of an enzyme catalyzed reaction (ordinate; against the reciprocal of the substrate concentration (abscissa; x-axis)? Lineweaver-Burk plot What shows maximum activity in vitro in the pH range of enzymes in plasma? pH from 7 to 8 What are the activity values observed in pH as low and high? Low as 1.5 (pepsin) High as 10.5 (ALP) What can be controlled by the use of buffer solutions? Pronounced effects of pH on enzyme reactions. When does enzymes inactivation and denaturation occurs? Occurs at temperature of 60 degrees Celsius to 70 degrees Celsius. What is the accepted measuring temperature for enzymes in the clinical laboratory? 37 degrees Celsius What is a substance that reduces the rate of an enzymatic reaction and is classified as reversible or irreversible? Inhibitor What is a structural analog of the substrate that combines with the free enzyme and competes with the normal substrate for binding at the active site? Competitive inhibitor What is structurally different from the substrate, it binds at a site on the enzyme molecule that is different from the site? Noncompetitive inhibitor
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