Figure 1: is a cut-away diagram of a modern upright phase contrast microscope, including a schematic illustration of the phase contrast optical train. Partially coherent illumination produced by the tungsten-halogen lamp is directed through a collector lens and focused on a (labeled condenser annulus) positioned in the specialized annulus substage condenser focal plane. and either pass Wave fronts passing through the annulus illuminate the specimen through by undeviated or are diffracted and retarded in phase structures and phase gradients present in the specimen. collected by the objective is by a Undeviated and diffracted segregated at the rear focal plane and focused at the intermediate to phase plate image plane form the final phase contrast image observed in the eyepieces. Figure 2 is a comparison of living cells in culture imaged in both brightfield and phase contrast illumination. The cells are human glial brain tissue
Figure 1: is a cut-away diagram of a modern upright phase contrast microscope, including a schematic illustration of the phase contrast optical train. Partially coherent illumination produced by the tungsten-halogen lamp is directed through a collector lens and focused on a specialized annulus (labeled condenser annulus) positioned in the substage condenser focal plane. Wave fronts passing through the annulus illuminate the specimen and either pass through undeviated or are diffracted and retarded in phase by structures and phase gradients present in the specimen. Undeviated and diffracted collected by the objective is segregated at the rear focal plane by a phase plate and focused at the intermediate image plane to form the final phase contrast image observed in the eyepieces. Figure 2 is a comparison of living cells in culture imaged in both brightfield and phase contrast illumination. The cells are human glial brain tissue
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