Clinical details/ Standard media Incubation Cultures Target organism(s) read conditions Temp. Time °C UTI CLED agar 35–37 Air 16–24hr Enterobacteriaceae ≥16hr Screening in or Enterococci pregnancy for Chromogenic Lancefield B asymptomatic agar streptococci bacteriuria by Pseudomonads culture S. saprophyticus Other staphylococci S. aureus Enteric fever Mannitol selenite 35–37 Air 16–24hr N/A S. Typhi screen* broth subcultured S. Paratyphi to: 16–24hr XLD 35–37 Air ≥16hr For situations, add the following: Clinical details/ Supplementary Incubation Cultures Target organism(s) media read conditions Temp. Atmos. Time °C Urine of patients Sabouraud agar 35–37 Air Fungi ≥40hr in Intensive Care, Special Care Baby Units, Burns Units and any from a Transplant Unit or if yeast have been seen in microscopy Multipoint culture Susceptibility 35–37 Air Antimicrobial ≥16hr using agar plates testing agar substances seeded with B.subtilis (NCTC 10400) Optional media Incubation Cultures Target organism(s) read Temp. Time °C If sterile pyuria Fastidious 35–37 anaerobic Anaerobes ≥40hr and no anaerobe agar Streptococci antimicrobials detected Chocolate agar 35–37 5-10% CO2 Fastidious organisms ≥40hr
Clinical details/ conditions Standard media Incubation Cultures read Target organism(s) Temp. °C Time UTI Screening in pregnancy for asymptomatic bacteriuria by culture CLED agar or Chromogenic agar 35–37 Air 16–24hr ≥16hr Enterobacteriaceae Enterococci Lancefield B streptococci Pseudomonads S. saprophyticus Other staphylococci S. aureus Enteric fever screen* Mannitol selenite broth subcultured to: XLD 35–37 35–37 Air Air 16–24hr 16–24hr N/A ≥16hr S. Typhi S. Paratyphi For situations, add the following: Clinical details/ conditions Supplementary media Incubation Cultures read Target organism(s) Temp. °C Atmos. Time Urine of patients in Intensive Care, Special Care Baby Units, Burns Units and any from a Transplant Unit or if yeast have been seen in microscopy Sabouraud agar 35–37 Air ≥40hr Fungi Multipoint culture using agar plates Susceptibility testing agar seeded with B.subtilis (NCTC 10400) 35–37 Air ≥16hr Antimicrobial substances Optional media Incubation Cultures read Target organism(s) Temp. °C Time If sterile pyuria and no antimicrobials detected Fastidious anaerobe agar 35–37 anaerobic ≥40hr Anaerobes Streptococci Chocolate agar 35–37 5-10% CO2 ≥40hr Fastidious organisms
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