Applications of phase contrast microscopy: It is preferable to bright field microscopy when (400x, 1000x) are needed high magnifications is widely used for examining such specimens as biological tissues. It is a type of by microscopy that enhances contrasts of transparent and colorless objects influencing the optical path of light. It is able to show components in a cell or bacteria, would be very difficult to see in an ordinary light microscope Figure. (a) organelles are nearly invisible in bright although they have different refractive indexes; (b) light is bent and retarded more by objects with a high refractive index; (c) in phase contrast a is phase plate placed in the light path. Barely refracted light passes through of and is the center the plate not retarded. Highly refracted light passes through the plate farther and is from center held back another one quarter wavelength.; (d) The microscope shows a (in this case the has a higher darker background cell cytoplasm refractive index than the contractile vacuole), with the organelles in sharp contrast
Applications of phase contrast microscopy: It is preferable to bright field microscopy when high magnifications (400x, 1000x) are needed is widely used for examining such specimens as biological tissues. It is a type of microscopy that enhances contrasts of transparent and colorless objects by influencing the optical path of light. It is able to show components in a cell or bacteria, would be very difficult to see in an ordinary light microscope Figure. (a) organelles are nearly invisible in bright although they have different refractive indexes; (b) light is bent and retarded more by objects with a high refractive index; (c) in phase contrast a phase plate is placed in the light path. Barely refracted light passes through the center of the plate and is not retarded. Highly refracted light passes through the plate farther from center and is held back another one quarter wavelength.; (d) The microscope shows a darker background (in this case the cell cytoplasm has a higher refractive index than the contractile vacuole), with the organelles in sharp contrast
Cell%20Biology%20Lab%20Manual.docx