S1. Oligonucleotide sequences used for qPCR assays and the source of DNA used for quantitative standards. Target gene Primer name Sequence Std curve sp. Source amoA-1F GGGGTTTCTACTGGTGGT amoA Rotthauwe et al. () Nitrosospira multiformis amoA-2R CCCCTCKGSAAAGCCTTCTTC nirK876 ATYGGCGGVAYGGCGA nirK Henry et al. () Pseudomonas fluorescens nirK1040 nirS-cd3aF GTSAACGTSAAGGARACSGG nirS Throback et al. () Pseudomonas fluorescens nirS-R3cd GASTTCGGRTGSGTCTTGA qnor-2F GGNCAYCARGGNTAYGA norB Braker and Tiedj () Alcaligenes faecalis qnor-5R ACCCANAGRTGNACNACCCACCA cnor-2F GACAAGNNNTACTGGTGGT Braker and Tiedj () Pseudomonas fluorescens cnor-7R TGNCCRTGNGCNGCNGT nosZ-F CGYTGTTCMTCGACAGCCAG nosZ Rosch et al. () Alcaligenes faecalis nosZ-R CATGTGCAGNGCRTGGCAGAA 16S rRNA 16S_Forward (1055f) ATGGCTGTCGTCAGCT et al.() Cloned Plasmid 16S_Reverse (1392r) ACGGGCGGTGTGTAC Zhang et al.() 16S_probe (16STaq1115) CAACGAGCGCAACCC
S1. Oligonucleotide sequences used for qPCR assays and the source of DNA used for quantitative standards. Target gene Primer name Sequence Std curve sp. Source amoA amoA-1F amoA-2R GGGGTTTCTACTGGTGGT CCCCTCKGSAAAGCCTTCTTC Nitrosospira multiformis Rotthauwe et al. () nirK nirK876 ATYGGCGGVAYGGCGA Pseudomonas fluorescens Henry et al. () nirK1040 nirS nirS-cd3aF GTSAACGTSAAGGARACSGG Pseudomonas fluorescens Throback et al. () nirS-R3cd GASTTCGGRTGSGTCTTGA norB qnor-2F GGNCAYCARGGNTAYGA Alcaligenes faecalis Braker and Tiedj () qnor-5R ACCCANAGRTGNACNACCCACCA cnor-2F GACAAGNNNTACTGGTGGT Pseudomonas fluorescens Braker and Tiedj () cnor-7R TGNCCRTGNGCNGCNGT nosZ nosZ-F CGYTGTTCMTCGACAGCCAG Alcaligenes faecalis Rosch et al. () nosZ-R CATGTGCAGNGCRTGGCAGAA 16S rRNA 16S_Forward (1055f) ATGGCTGTCGTCAGCT Cloned Plasmid et al.() 16S_Reverse (1392r) ACGGGCGGTGTGTAC Zhang et al.() 16S_probe (16STaq1115) CAACGAGCGCAACCC
Chen-Bioretention-2013-SI.docx