For newborn screening, whole blood may be 48 to 72 collected by heel puncture how many hours after birth. Secretion of TSH is circadian with big 2:00 to 4:00 AM concentrations occurring between ______(time 5:00 to 6:00 PM of day), and the lowest between _______(time of day). Measurement is done using electron capture gas Thyroxine (T4) chromatography, high performance liquid chromatography, and isotope dilution tandem mass spectrometry. This is the Measurement of what? What is the preferred specimen (EDTA and Serum heparin plasma can also be used) for Total Thyroxine . Because total T4 alone provides limited clinical True information, FT4 measurements are preferred. True or False? What does T3 stand for? Triiodothyronine What is the techniques of choice to measure T3 Immunoassays in body fluids predominantly or plasma? Give 2 examples. Radio immunoassay Non-isotopic immunoassays Serum specimens should be tested within 24 ______ hours of collection. What temperature must serum specimens be 2 to 8°C stored at after 24 hours? What does stand for? Reverse Triiodothyronine ‘rT3’ Measurement are done, using which Radio immunoassay immunoassay? rT3 measurement has limited True True or False: diagnostic value. True or False: The diagnosis of non-thyroidal True illnesses can usually be established without measuring rT3. What are the methods of measurement of Free Direct assays Thyroid Hormones? as reference methods •serve Equilibrium Dialysis –Direct –Ultrafiltration Indirect or estimate assays general laboratory use •for and One-step immunoassay –Two-step What type of measurement are used in heterogenous method •competitive, Thyroxine Binding Globulin (TBG)? of bound conjugate by •measurement chemiluminescence microparticle turbidimetry •enhance
For newborn screening, whole blood may be collected by heel puncture how many hours after birth. 48 to 72 Secretion of TSH is circadian with big concentrations occurring between ______(time of day), and the lowest between _______(time of day). 2:00 to 4:00 AM 5:00 to 6:00 PM Measurement is done using electron capture gas chromatography, high performance liquid chromatography, and isotope dilution tandem mass spectrometry. This is the Measurement of what? Thyroxine (T4) What is the preferred specimen (EDTA and heparin plasma can also be used) for Total Thyroxine . Serum Because total T4 alone provides limited clinical information, FT4 measurements are preferred. True or False? True What does T3 stand for? Triiodothyronine What is the techniques of choice to measure T3 in body fluids predominantly or plasma? Immunoassays Give 2 examples. Radio immunoassay Non-isotopic immunoassays Serum specimens should be tested within ______ hours of collection. 24 What temperature must serum specimens be stored at after 24 hours? 2 to 8°C What does ‘rT3’ stand for? Reverse Triiodothyronine Measurement are done, using which immunoassay? Radio immunoassay True or False: rT3 measurement has limited diagnostic value. True True or False: The diagnosis of non-thyroidal illnesses can usually be established without measuring rT3. True What are the methods of measurement of Free Thyroid Hormones? Direct assays •serve as reference methods –Direct Equilibrium Dialysis –Ultrafiltration Indirect or estimate assays •for general laboratory use –Two-step and One-step immunoassay What type of measurement are used in Thyroxine Binding Globulin (TBG)? •competitive, heterogenous method •measurement of bound conjugate by chemiluminescence •enhance microparticle turbidimetry
ChemPPT%20Flashcards%20Unit%204.docx