B-antigen: extra Gal (Galactose) DNA BASES PURINES AND PYRIMIDINES – 4 differen bases in DNA. Bicyclic purines (A, G) – Monocyclic pyrimidines (C, T, U for RNA) – NUCLEOSIDE: Nucleobase + ribose Adenine adenosine deoxyadenosine Guanine guanosine deoxyguanosine Cytosine cytidine deoxycytidine Uracil uridine Thymine thymidine deoxythymidine Deoxynucleosides have no 2’ OH NUCELOTIDE: base +ribose + phosphate Condensation of phosphate group on one nucleotide and the atom of the next nucleotide = –OH phosphodiester bridge linking nucleosides. 3 hydrogen bonds between C and G 2 hydrogen between A and T OLIGONUCLEOTIDES Will bind to complementary sequences of single stranded DNA (or RNA) HYBRIDISATION – LIPIDS Water soluble, polar head group and non-polar tail.
B-antigen: extra Gal (Galactose) DNA BASES – PURINES AND PYRIMIDINES 4 differen bases in DNA. Bicyclic – purines (A, G) Monocyclic – pyrimidines (C, T, U for RNA) NUCLEOSIDE: Nucleobase + ribose Adenine adenosine deoxyadenosine Guanine guanosine deoxyguanosine Cytosine cytidine deoxycytidine Uracil uridine Thymine thymidine deoxythymidine Deoxynucleosides have no 2’ OH NUCELOTIDE: base +ribose + phosphate Condensation of phosphate group on one nucleotide and the –OH atom of the next nucleotide = phosphodiester bridge linking nucleosides. 3 hydrogen bonds between C and G 2 hydrogen between A and T OLIGONUCLEOTIDES Will bind to complementary sequences of single stranded DNA (or RNA) – HYBRIDISATION LIPIDS Water soluble, polar head group and non-polar tail.
CBB-Notes.docx