In order to improve this research method and results to a better concentration of glucose, it can be carried out in various modifications on these parameters. The concentration of glucose using the method of enzymatic hydrolysis can also be done using the method of acid hydrolysis which is cheaper in terms of the cost of raw material. Besides using only single enzyme, the concentration of glucose can be increased by mixing the three commercialized enzymes (cellulose, glucoamylase and There are also differences when using immobilize enzyme and free α-amylase). enzyme in concentration of glucose which can be studied to compare the concentration between the two types of enzymes. The concentration of glucose was analyzing using DNS reagent. But for future studies, analyzing the samples using High Performance Liquid Chromatography (HPLC) can gives more accurate concentration of glucose. In this study, the substrate used is cassava residue. In future, to study the concentration of substrate will give the significant in concentration of glucose. Moreover, there are more substrate other than cassava residue that are also capable of producing sugar such as Cotton Stalk, Corn Stover, Wheat Straw, Tobacco Stalk and Palm Oil Empty Fruit Bunch. This research can also be done by varying the agitation rate and the reaction temperature incubating. The pH of the buffer may give effect on the productions. Therefore, this can also be study by varying the pH of buffer. In addition, other kind of buffer can also be used such as Potassium Phosphate and Ampso. REFERENCES
In order to improve this research method and results to a better concentration of glucose, it can be carried out in various modifications on these parameters. The concentration of glucose using the method of enzymatic hydrolysis can also be done using the method of acid hydrolysis which is cheaper in terms of the cost of raw material. Besides using only single enzyme, the concentration of glucose can be increased by mixing the three commercialized enzymes (cellulose, glucoamylase and α-amylase). There are also differences when using immobilize enzyme and free enzyme in concentration of glucose which can be studied to compare the concentration between the two types of enzymes. The concentration of glucose was analyzing using DNS reagent. But for future studies, analyzing the samples using High Performance Liquid Chromatography (HPLC) can gives more accurate concentration of glucose. In this study, the substrate used is cassava residue. In future, to study the concentration of substrate will give the significant in concentration of glucose. Moreover, there are more substrate other than cassava residue that are also capable of producing sugar such as Cotton Stalk, Corn Stover, Wheat Straw, Tobacco Stalk and Palm Oil Empty Fruit Bunch. This research can also be done by varying the agitation rate and the reaction temperature incubating. The pH of the buffer may give effect on the productions. Therefore, this can also be study by varying the pH of buffer. In addition, other kind of buffer can also be used such as Potassium Phosphate and Ampso. REFERENCES
CD5904_MOHD_SHAHRIL_MUSTAFA.docx