Appendix B. Results of analyses of population genetic structure of in lectularius the North American Great Lakes region using two different microsatellite datasets. Appendix B1. Spatial genetic structure, diversity, and differentiation in Cimex lectularius samples, estimated using two different panels of microsatellite markers varying in the number of markers. Most likely number of genetic clusters (K) estimated using the Geneland method, Isolation-by-distance (IBD) and IBD while correcting for population genetic structure (IBD + average expected heterozygosity (HS), Hedrick’s global K), differentiation index (and 95% confidence intervals), and AMOVA = Analysis of G’ST Molecular Variance, are shown. Number of markers 20 7 Most likely 13 11 K 0.001* 0.001* P IBD (rW) 0.11 0.09 R2 (IBD) 0.001* 0.001* P (K) 0.001* 0.001* P IBD (rW) + K 0.26 0.22 R2 0.320 (0.289 0.351) 0.351 (0.292 0.410) HS – – Hedrick’s 0.746 (0.711 0.781) 0.750 (0.683 0.817) G’ST – – Within 33.1 42.1 AMOVA (% of variation) Among sites 66.9 57.9 *Statistically significant at = 0.05 α
Appendix B. Results of analyses of population genetic structure of lectularius in the North American Great Lakes region using two different microsatellite datasets. Appendix B1. Spatial genetic structure, diversity, and differentiation in Cimex lectularius samples, estimated using two different panels of microsatellite markers varying in the number of markers. Most likely number of genetic clusters (K) estimated using the Geneland method, Isolation-by-distance (IBD) and IBD while correcting for population genetic structure (IBD + K), average expected heterozygosity (HS), Hedrick’s global G’ST differentiation index (and 95% confidence intervals), and AMOVA = Analysis of Molecular Variance, are shown. Number of markers 20 7 Most likely K 13 11 IBD (rW) P 0.001* 0.001* R2 0.11 0.09 IBD (rW) + K P (IBD) 0.001* 0.001* P (K) 0.001* 0.001* R2 0.26 0.22 HS 0.320 (0.289 – 0.351) 0.351 (0.292 – 0.410) Hedrick’s G’ST 0.746 (0.711 – 0.781) 0.750 (0.683 – 0.817) AMOVA (% of variation) Within 33.1 42.1 Among sites 66.9 57.9 *Statistically significant at α = 0.05
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