Appendix 2: Published evaluations of media / methods for detecting producing Enterobacteriaceae Published evaluations of media / methods for detecting carbapenemase-producing Enterobacteriaceae in various patient populations Sensitivity Specificity No. of positive Reference Media / Method tested Comments (%) (%) location Samples/Total Samples CHROMagar KPC 84.9 88.7 18 MacConkey plus carbapenem discs 75.8 89.6 Israel 33 / 139 All isolates of CPE had KPC enzyme. MacConkey plus imipenem (1 mg/L) 84.9 94.3 CHROMagar KPC 100 98.4 Sensitivity and specificity of both were calculated relative to PCR 19 Israel 41 / 122 testing of samples. All isolates of CPE had KPC-3 enzyme MacConkey plus carbapenem discs. 92.7 95.9 CHROMagar KPC 97.8 NDa 20 Greece 46 / 126 Predominant carbapenemases were KPC and VIM MacConkey plus imipenem (1 mg/L) 78.3 ND Colorex KPC 97 96 22 Pakistan 37 / 200 Prototype version of chromID CARBA tested. All CPE had NDM-1 chromID 100 93 chromID CARBA 100 98 23 Pakistan 32 / 175 All CPE had NDM-1 Brilliance CRE 62.5 34 TSB plus ertapenem (2 mg/L) 89.1 86.4 chromID ESBL 92.4 93.3 24 Greece 73 / 200 Predominant carbapenemases were KPC and VIM chromID ESBL (plus enrichment) 92.4 84.7 chromID 92.4 96.9 MacConkey plus meropenem (1 mg/L) 89.1 85.2 CHROMagar ESBL 77.3 100 27 USA 66 / 95 All isolates of CPE had KPC enzyme 77.3 100 PCR for blaKPC 97 96.6 CHROMagar KPC 76.6 75.7 30 USA Remel SPECTRA CRE 97.8 86.4 MacConkey plus ertapenem disc 83 73.8 47 / 150 All isolates of CPE had KPC enzyme
Appendix 2: Published evaluations of media / methods for detecting Enterobacteriaceae Published evaluations of media / methods for detecting carbapenemase-producing Enterobacteriaceae in various patient populations Reference Media / Method tested Sensitivity (%) Specificity (%) location No. of positive Samples/Total Samples Comments 18 CHROMagar KPC 84.9 88.7 Israel 33 / 139 All isolates of CPE had KPC enzyme. MacConkey plus carbapenem discs 75.8 89.6 MacConkey plus imipenem (1 mg/L) 84.9 94.3 19 CHROMagar KPC 100 98.4 Israel 41 / 122 Sensitivity and specificity of both were calculated relative to PCR testing of samples. All isolates of CPE had KPC-3 enzyme MacConkey plus carbapenem discs. 92.7 95.9 20 CHROMagar KPC 97.8 NDa Greece 46 / 126 Predominant carbapenemases were KPC and VIM MacConkey plus imipenem (1 mg/L) 78.3 ND 22 Colorex KPC 97 96 Pakistan 37 / 200 Prototype version of chromID CARBA tested. All CPE had NDM-1 chromID 100 93 23 chromID CARBA 100 98 Pakistan 32 / 175 All CPE had NDM-1 Brilliance CRE 62.5 34 24 TSB plus ertapenem (2 mg/L) 89.1 86.4 Greece 73 / 200 Predominant carbapenemases were KPC and VIM chromID ESBL 92.4 93.3 chromID ESBL (plus enrichment) 92.4 84.7 chromID 92.4 96.9 MacConkey plus meropenem (1 mg/L) 89.1 85.2 27 CHROMagar ESBL 77.3 100 USA 66 / 95 All isolates of CPE had KPC enzyme 77.3 100 PCR for blaKPC 97 96.6 30 CHROMagar KPC 76.6 75.7 USA 47 / 150 All isolates of CPE had KPC enzyme Remel SPECTRA CRE 97.8 86.4 MacConkey plus ertapenem disc 83 73.8 producing
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