conditions Temp Atmos Time Cultures °C read bacteraemia 5-10% cfu per plate of ≥15 Cannulae agar 35-37 24-48hr Daily CO2 any bacteria infection Coagulase negative staphylococci S. aureus including MRSA* Corynebacterium species Local cannula 5-10% Enterobacteriaceae Swabs Blood agar 35-37 24-48hr Daily site infection CO2 Enterococci Pseudomonads Streptococci Bacillus species Yeasts# For these situations, add the following: Clinical details/ Specimen Incubation Cultures Target organism(s) read conditions Optional media Temp Time °C (after Sabouraud agar 28-30 Air Up to 2d and at Yeasts** related flagging supplemented with 7d 5d fungaemia positive on chloramphenicol blood and gentamicin or culture chromogenic automated agar58,59 systems) Cannulae Peripheral Coagulase negative related IV line tips staphylococci bacteraemia there S. aureus including is a positive MRSA* blood culture in Corynebacterium infection the 7 days species before or 5-10% agar 35-37 24-48hr Daily Enterobacteriaceae after the CO2 day the line Enterococci tip is sent)10,60 Pseudomonads Streptococci Bacillus species Yeasts# * For more information on MRSA, refer to . ** Some yeasts can grow at much higher temperatures such as Malassezia species which grow strictly at 30-35°C and not outside this range61 . # Any cfu growth of yeasts is considered significant and should be reported. For appearance of relevant target organisms, refer to individual SMIs for organism identification. ‡
conditions Cultures read Temp °C Atmos Time bacteraemia infection Cannulae agar 35-37 5-10% CO2 24-48hr Daily ≥15 cfu per plate of any bacteria Local cannula site infection Swabs Blood agar 35-37 5-10% CO2 24-48hr Daily Coagulase negative staphylococci S. aureus including MRSA* Corynebacterium species Enterobacteriaceae Enterococci Pseudomonads Streptococci Bacillus species Yeasts# For these situations, add the following: Clinical details/ conditions Specimen Optional media Incubation Cultures read Target organism(s) Temp °C Time fungaemia (after flagging positive on blood culture automated systems) Cannulae Sabouraud agar supplemented with chloramphenicol and gentamicin or chromogenic agar58,59 28-30 Air Up to 7d 2d and at 5d Yeasts** bacteraemia infection Peripheral IV line tips there is a positive blood culture in the 7 days before or after the day the line tip is sent)10,60 agar 35-37 5-10% CO2 24-48hr Daily Coagulase negative staphylococci S. aureus including MRSA* Corynebacterium species Enterobacteriaceae Enterococci Pseudomonads Streptococci Bacillus species Yeasts# * For more information on MRSA, refer to . ** Some yeasts can grow at much higher temperatures such as Malassezia species which grow strictly at 30-35°C and not outside this range61 . # Any cfu growth of yeasts is considered significant and should be reported. ‡ For appearance of relevant target organisms, refer to individual SMIs for organism identification. related related
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