Clinical Standard Incubation Cultures Target organism(s) details/ media read conditions Temp Atmos Time °C All clinical Blood agar† 35-37 5-10% CO2 Daily Any organism may be conditions significant Fastidious 35-37 anaerobic 40hr and Any organism may be anaerobe agar up to 5d significant For these situations add the following: Clinical Supplementary Incubation Cultures Target organism(s) details/ read conditions Temp Atmos Time °C Suspected Chocolate agar† 35-37 5-10% CO2 40-48hr Daily Haemophilus species meningo- N. meningitidis coccaemia or meningitis N. gonorrhoeae Gram negative rods or diplococci seen on microscopy Gram negative MacConkey/ 35-37 air 16-24hr 16hr Enterobacteriaceae rods seen on CLED agar or Non–fermentative organism microscopy Chromogenic agar Pseudomonas species Microscopy Neomycin 35-37 anaerobic 5-7d 40hr and Anaerobes suggestive of fastidious at 5d mixed or anaerobe agar anaerobic with infection metronidazole 5g disc Systemic Sabouraud’s 28-30 air 5d 2d and Yeast fungal agar at 5d Mould infection# Clinical details/ Supplementary Incubation Cultures Target organism(s) conditions media read Temp Atmos Time °C
Clinical details/ conditions Standard media Incubation Cultures read Target organism(s) Temp °C Atmos Time All clinical conditions Blood agar† 35-37 5-10% CO2 Daily Any organism may be significant Fastidious anaerobe agar 35-37 anaerobic 40hr and up to 5d Any organism may be significant For these situations add the following: Clinical details/ conditions Supplementary Incubation Cultures read Target organism(s) Temp °C Atmos Time Suspected meningo- coccaemia or meningitis Gram negative rods or diplococci seen on microscopy Chocolate agar† 35-37 5-10% CO2 40-48hr Daily Haemophilus species N. meningitidis N. gonorrhoeae Gram negative rods seen on microscopy MacConkey/ CLED agar or Chromogenic agar 35-37 air 16-24hr 16hr Enterobacteriaceae Non–fermentative organism Pseudomonas species Microscopy suggestive of mixed or anaerobic infection Neomycin fastidious anaerobe agar with metronidazole 5g disc 35-37 anaerobic 5-7d 40hr and at 5d Anaerobes Systemic fungal infection# Sabouraud’s agar 28-30 air 5d 2d and at 5d Yeast Mould Clinical details/ conditions Supplementary media Incubation Cultures read Target organism(s) Temp °C Atmos Time
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