the information presented about Serial Dilutions. information from the Resource Sheet to complete practice problems A-C found at the bottom of the Resource Sheet. your answers with a partner. Correct any mistakes. Conclusion question 3. Part III: Running an ELISA Use the ELISA test to determine which patients test positive for the presence of the disease antigen and to determine the relative concentration of the bacteria in the fluid of those who are affected. Early findings assure adequate treatment to those who are affected and provide information necessary to prevent the disease from spreading all over the college. In this lab activity, you will be testing the simulated CSF of two of the patients presented in Activity 1.1.2 and Activity 1.1.3. These samples have randomly been assigned by your teacher. on your safety goggles. two 12-well strips at your lab station. one strip off to the side. You will use one strip to prepare your concentration standards and one strip to complete your ELISA test. a marker to label the outside wall of each well on one 12-well strip with the numbers 1-12. This strip will serve as your concentration standards. a micropipettor to add to 50µl of Phosphate Buffered Saline (PBS) from the tube labeled PBS to the labeled #2-#12. from the tube labeled 1,000 ng/ml antigen (AG) to the well labeled #1. prepare the various concentration standards, complete the serial dilutions as described below. Pipet 50µl out of well #1 and add it to well #2. Pipet up and down gently three times to mix the sample in well #2. Using the same pipet tip, transfer 50µl from well #2 to well #3 and mix the sample in well #3 Continue this process with each of the remaining Stop when you reach well #11. Remove 50µl of fluid from well #11 and discard this fluid into a waste container. Do not add additional liquid to well #12. a diagram of the 12-well plate in your laboratory journal. the diagram, calculate the dilution in each well. Think about what you have added to each well and about the practice serial dilution questions you completed in Part II. that the concentration of antigen in well #1 is 1000 ng/ml. Calculate the concentration of antigen in wells 2-12. Show your work in your laboratory journal.
the information presented about Serial Dilutions. information from the Resource Sheet to complete practice problems A-C found at the bottom of the Resource Sheet. your answers with a partner. Correct any mistakes. Conclusion question 3. Part III: Running an ELISA Use the ELISA test to determine which patients test positive for the presence of the disease antigen and to determine the relative concentration of the bacteria in the fluid of those who are affected. Early findings assure adequate treatment to those who are affected and provide information necessary to prevent the disease from spreading all over the college. In this lab activity, you will be testing the simulated CSF of two of the patients presented in Activity 1.1.2 and Activity 1.1.3. These samples have randomly been assigned by your teacher. on your safety goggles. two 12-well strips at your lab station. one strip off to the side. You will use one strip to prepare your concentration standards and one strip to complete your ELISA test. a marker to label the outside wall of each well on one 12-well strip with the numbers 1-12. This strip will serve as your concentration standards. a micropipettor to add to 50µl of Phosphate Buffered Saline (PBS) from the tube labeled PBS to the labeled #2-#12. from the tube labeled 1,000 ng/ml antigen (AG) to the well labeled #1. prepare the various concentration standards, complete the serial dilutions as described below. Pipet 50µl out of well #1 and add it to well #2. Pipet up and down gently three times to mix the sample in well #2. Using the same pipet tip, transfer 50µl from well #2 to well #3 and mix the sample in well #3 Continue this process with each of the remaining Stop when you reach well #11. Remove 50µl of fluid from well #11 and discard this fluid into a waste container. Do not add additional liquid to well #12. a diagram of the 12-well plate in your laboratory journal. the diagram, calculate the dilution in each well. Think about what you have added to each well and about the practice serial dilution questions you completed in Part II. that the concentration of antigen in well #1 is 1000 ng/ml. Calculate the concentration of antigen in wells 2-12. Show your work in your laboratory journal.
1.1.5.a_elisa-1.docx