4.5.1 Culture media, conditions and organisms Clinical Specimen Standard Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C Direct MRSA Chromogenic 37 Aerobic daily MRSA culture screening selective MRSA specimens medium AND/OR Enrichment Nutrient broth 30 Aerobic 18-24hr N/A culture containing 2.5% NaCl *** then subculture to (see below) Chromogenic 37 Aerobic daily MRSA selective MRSA medium * Consider a molecular method if results are required. **For chromogenic refer to manufacturer’s instructions for recommended incubation times. ***The bottle should contain a volume of broth sufficient to cover the swabs. The NaCl concentration should be reduced if locally prevalent strains are known to be inhibited by 2.5% NaCl. 4.6 Identification 4.6.1 Minimum level of identification in the laboratory S. aureus species level, meticillin resistant. Organisms may be further identified if this is clinically or epidemiologically indicated. 4.7 Antimicrobial Susceptibility Testing to and/or guidelines. 4.8 Referral for Outbreak Investigations N/A
4.5.1 Culture media, conditions and organisms Clinical details/ conditions Specimen Standard media Incubation Cultures read Target organism(s) Temp °C Atmos Time Direct culture MRSA screening specimens Chromogenic selective MRSA medium 37 Aerobic daily MRSA AND/OR Enrichment culture Nutrient broth containing 2.5% NaCl *** then subculture to (see below) 30 Aerobic 18-24hr N/A Chromogenic selective MRSA medium 37 Aerobic daily MRSA * Consider a molecular method if results are required. **For chromogenic refer to manufacturer’s instructions for recommended incubation times. ***The bottle should contain a volume of broth sufficient to cover the swabs. The NaCl concentration should be reduced if locally prevalent strains are known to be inhibited by 2.5% NaCl. 4.6 Identification 4.6.1 Minimum level of identification in the laboratory S. aureus species level, meticillin resistant. Organisms may be further identified if this is clinically or epidemiologically indicated. 4.7 Antimicrobial Susceptibility Testing to and/or guidelines. 4.8 Referral for Outbreak Investigations N/A
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