All clinical Bone Blood culture 35 37 Air 5 d Continuous Any – conditions marrow broths monitoring organism + terminal (aerobic and subculture anaerobic) Subculture all bottles onto subculture plates below. Subculture Bone agar 35 37 5 10% 40 48hr Any – – – ≥40hr marrow CO2 organism plates Chocolate 35 - 37 5 10% 40 - 48hr* Any – ≥40hr agar CO2 organism FAA 35-37 Anaerobic 5 d 3 d and 5 d Anaerobes For these situations, add the following: Clinical Specimen Incubation Cultures Target details/ media read organism(s) Temp Atmos Time conditions °C Systemic Bone Sabouraud 28 - 30 Air 14 d Daily Yeast and fungal marrow agar Mould infection (slopes) Where Bone Direct clinically marrow Culture: indicated Blood agar 35 37 5 10% 40 48hr Any – – – ≥40hr CO2 organism Chocolate 5 10% Any – 35 37 40 - 48hr* – ≥40hr agar CO2 organism 35-37 5 d 3 d and 5 d FAA Anaerobic Anaerobes Optional Molecular Techniques Clinical Specimen Molecular Instructions Target details/ Technique organism(s) conditions All clinical Bone NAAT Follow manufacturer’s instructions Any conditions marrow organism Other organisms for consideration - Mycobacterium species (see ), fungi, parasites (see ) and viruses (see ). *Incubation times may be increased up to 5 days if Brucella species infection is suspected. 4.6 Identification Refer to individual SMIs for organism identification. 4.6.1 Minimum of identification in the laboratory All organisms to species level. Organisms may be further identified if this is clinically or epidemiologically indicated.
All clinical conditions Bone marrow Blood culture broths (aerobic and anaerobic) Subculture all bottles onto subculture plates below. 35 – 37 Air 5 d + terminal subculture Continuous monitoring Any organism Subculture plates Bone marrow agar 35 – 37 5 – 10% CO2 40 – 48hr ≥40hr Any organism Chocolate agar 35 - 37 5 – 10% CO2 40 - 48hr* ≥40hr Any organism FAA 35-37 Anaerobic 5 d 3 d and 5 d Anaerobes For these situations, add the following: Clinical details/ conditions Specimen media Incubation Cultures read Target organism(s) Temp °C Atmos Time Systemic fungal infection Bone marrow Sabouraud agar (slopes) 28 - 30 Air 14 d Daily Yeast and Mould Where clinically indicated Bone marrow Direct Culture: Blood agar Chocolate agar FAA 35 – 37 35 – 37 35-37 5 – 10% CO2 5 – 10% CO2 Anaerobic 40 – 48hr 40 - 48hr* 5 d ≥40hr ≥40hr 3 d and 5 d Any organism Any organism Anaerobes Optional Molecular Techniques Clinical details/ conditions Specimen Molecular Technique Instructions Target organism(s) All clinical conditions Bone marrow NAAT Follow manufacturer’s instructions Any organism Other organisms for consideration - Mycobacterium species (see ), fungi, parasites (see ) and viruses (see ). *Incubation times may be increased up to 5 days if Brucella species infection is suspected. 4.6 Identification Refer to individual SMIs for organism identification. 4.6.1 Minimum of identification in the laboratory All organisms to species level. Organisms may be further identified if this is clinically or epidemiologically indicated.
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