growth (≥40hr), or at day 5 to 35-37 As above 40hr above media (excluding MacConkey) For these situations, add the following: Clinical Specimen Supplementary Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C Pus Chocolate agar 35–37 5–10% 40 40hr Fastidious – abscess CO2 organisms Brain abscess Liver abscess Lung empyema/ abscess Psoas abscess Spinal abscess Actinomycosis Pus Blood agar 35-37 Anaerobic 10 d 40hr, at Actinomyces supplemented 7 d and species (or where with 10 d microscopy metronidazole suggestive of and nalidixic acid Nocardiosis Pus Blood agar 35-37 5-10% daily Nocardia species* CO2 Pus Sabouraud agar 28-30 Air 14 d daily Yeast or Mould fungi suspected (Gram film or clinical details) Prostatic Pus GC selective/ 35-37 5-10% 40hr N. gonorrhoeae abscess Chocolate agar CO2 Primary peritonitis in females Clinical Specimen Optional Incubation Cultures Target organism(s) details/ read Temp Atmos Time conditions °C When clinical Staph/strep Pus 35-37 Air daily S. aureus details or selective agar Streptococci microscopy suggestive of
growth (≥40hr), or at day 5 to above media (excluding MacConkey) 35-37 As above 40hr For these situations, add the following: Clinical details/ conditions Specimen Supplementary media Incubation Cultures read Target organism(s) Temp °C Atmos Time abscess Brain abscess Liver abscess Lung empyema/ abscess Psoas abscess Spinal abscess Pus Chocolate agar 35–37 5–10% CO2 40 – 40hr Fastidious organisms Actinomycosis (or where microscopy suggestive of Pus Blood agar supplemented with metronidazole and nalidixic acid 35-37 Anaerobic 10 d 40hr, at 7 d and 10 d Actinomyces species Nocardiosis Pus Blood agar 35-37 5-10% CO2 daily Nocardia species* or fungi suspected (Gram film or clinical details) Pus Sabouraud agar 28-30 Air 14 d daily Yeast Mould Prostatic abscess Primary peritonitis in females Pus GC selective/ Chocolate agar 35-37 5-10% CO2 40hr N. gonorrhoeae Clinical details/ conditions Specimen Optional Incubation Cultures read Target organism(s) Temp °C Atmos Time When clinical details or microscopy suggestive of Pus Staph/strep selective agar 35-37 Air daily S. aureus Streptococci
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