Table S1. Characteristics of primers and of fragments isolated by the three primer pairs on 320 individuals across 13 island populations and three mainland populations of in the Iris versicolor Bay of Fundy. Primer Sequence Tm Motif Fragment size Number of Percent of Distinct first range different individuals combinations (C°) isolated fragment amplifying of fragment lengths for a single lengths fragment Tet4F CTAGCCTCGACGCTTTTCC 64 agc6 224-272 15 0.0 65 GCTGCAGCCTCAGCAGTAGT 65 Tet9F GTCCATCATGCCAAGTACGA 64 cata8 221-257 10 4.8 93 Tet9R AACGCGAGTTTCCAAACATA 62 IM200F AACGGAAATGGCGAATAAACT 57 350-374 9 1.6 68 63 _______________________________________________________________________________________
Table S1. Characteristics of primers and of fragments isolated by the three primer pairs on 320 individuals across 13 island populations and three mainland populations of Iris versicolor in the Bay of Fundy. Primer Sequence Tm (C°) Motif first isolated Fragment size range Number of different fragment lengths Percent of individuals amplifying for a single fragment Distinct combinations of fragment lengths Tet4F CTAGCCTCGACGCTTTTCC 64 agc6 224-272 15 0.0 65 GCTGCAGCCTCAGCAGTAGT 65 Tet9F GTCCATCATGCCAAGTACGA 64 cata8 221-257 10 4.8 93 Tet9R AACGCGAGTTTCCAAACATA 62 IM200F AACGGAAATGGCGAATAAACT 57 350-374 9 1.6 68 63 _______________________________________________________________________________________
cjb-2016-0004-supplement-1.docx