4.5.1 Culture conditions and organisms Clinical Specimen Standard Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C Cholangitis Bile Blood agar 35-37 5-10% 40-48hr daily CO2 Cholecystitis Any organism CLED*/ 35-37 air 16-24hr 16hr MacConkey agar Neomycin 35-37 anaerobic 40-48hr ** 48hr*** Anaerobes fastidious anaerobe agar For these situations, add the following: Clinical Specimen Supplementary Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C Salmonella Bile Mannitol 35-37 air 16-24hr N/A Salmonella species selenite F broth then 35-37 air 16-24hr 16hr subcultured to XLD * CLED agar, originally designed for specimens ** Prolonged 14-day incubation might be of interest in particular situations in which the prevalence of slow-growing microorganisms and anaerobes is higher; in such cases plates should be read at 5 days and then left in the incubator/cabinet until day 1436 *** if the laboratory has an anaerobic cabinet plates may be read at 48 hours, otherwise they should be left for 5 to 7 days 4.6 Identification Refer to individual UK SMIs for organism identification. 4.6.1 Minimum level of identification in the laboratory Note: All work on S. Typhi and S. Paratyphi A, B & C must be performed in a microbiological safety cabinet in a Containment Level 3 room. Anaerobes "anaerobes" level Lancefield group level "coagulase negative" level "coliforms" level genus level species level "pseudomonads" level S. Typhi, S. Paratyphi or other serogroup level Whole genome sequencing 37 species level
4.5.1 Culture conditions and organisms Clinical details/ conditions Specimen Standard media Incubation Cultures read Target organism(s) Temp °C Atmos Time Cholangitis Cholecystitis Bile Blood agar 35-37 5-10% CO2 40-48hr daily Any organism CLED*/ MacConkey agar 35-37 air 16-24hr 16hr Neomycin fastidious anaerobe agar 35-37 anaerobic 40-48hr ** 48hr*** Anaerobes For these situations, add the following: Clinical details/ conditions Specimen Supplementary media Incubation Cultures read Target organism(s) Temp °C Atmos Time Salmonella Bile Mannitol selenite F broth then subcultured to XLD 35-37 35-37 air air 16-24hr 16-24hr N/A 16hr Salmonella species * CLED agar, originally designed for specimens ** Prolonged 14-day incubation might be of interest in particular situations in which the prevalence of slow-growing microorganisms and anaerobes is higher; in such cases plates should be read at 5 days and then left in the incubator/cabinet until day 1436 *** if the laboratory has an anaerobic cabinet plates may be read at 48 hours, otherwise they should be left for 5 to 7 days 4.6 Identification Refer to individual UK SMIs for organism identification. 4.6.1 Minimum level of identification in the laboratory Note: All work on S. Typhi and S. Paratyphi A, B & C must be performed in a microbiological safety cabinet in a Containment Level 3 room. Anaerobes "anaerobes" level Lancefield group level "coagulase negative" level "coliforms" level genus level species level "pseudomonads" level S. Typhi, S. Paratyphi or other serogroup level Whole genome sequencing 37 species level
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