Table 4 P. aeruginosa lectin binding and biofilm inhibition by glycopeptide dendrimers IC50, μM No Structurea Lectin (ELLA)b KD, (ITC)c inhibitione μM LecB 5.27 ± 2.1 α-NPF α-(p-Nitrophenyl)-L-fucoside — 0.55 T1 (cFuc-RL)2BRIFV LecB 5 ± 1.7 n.d. 0.45 KT1 37 (cFuc)4(KRL)2BRIFV LecB 0.59 ± 7.2 n.d. 0.059 2G0 cFuc-KPL LecB 5.94 ± 1.9 n.d. 1.24 2G1 LecB 2.7 ± 2.0 n.d. 0.56 37 (cFuc-KPL)4(KFKI)2KHI LecB 0.14 ± 20 ++++ 0.035 D-36 (cFuc-kpl)4(Kfkl)2Khl LecB 0.66 ± 4.2 +++ 0.12 2G3 (cFuc-KP)8(KLF)4(KKI)2KHI LecB 0.025 55 n.d. ± 0.005 NPG p-Nitrophenyl LecA 14.1 ± 6.2 — 0.2 GalAG0 GalA-KPL LecA 4.2 ± 0.9 21 + GalAG1 (GalA-KPL)2KFKI LecA 0.5 ± 0.2 91 ++ GalAG2 (GalA-KPL)4(KFKI)2KHI LecA 0.1 ± 220 +++ 33 0.01 GalBG0 GalB-KPL LecA 51.5 ± 1.7 + 6.7 GalBG1 (GalB-KPL)2KFKI LecA 2.1 ± 1.0 21 ++
Table 4 P. aeruginosa lectin binding and biofilm inhibition by glycopeptide dendrimers No Structurea Lectin IC50, μM (ELLA)b KD, μM (ITC)c inhibitione α-NPF α-(p-Nitrophenyl)-L-fucoside LecB 5.27 ± 0.55 2.1 — T1 (cFuc-RL)2BRIFV LecB 5 ± 0.45 1.7 n.d. KT1 37 (cFuc)4(KRL)2BRIFV LecB 0.59 ± 0.059 7.2 n.d. 2G0 cFuc-KPL LecB 5.94 ± 1.24 1.9 n.d. 2G1 LecB 2.7 ± 0.56 2.0 n.d. 37 (cFuc-KPL)4(KFKI)2KHI LecB 0.14 ± 0.035 20 ++++ D-36 (cFuc-kpl)4(Kfkl)2Khl LecB 0.66 ± 0.12 4.2 +++ 2G3 (cFuc-KP)8(KLF)4(KKI)2KHI LecB 0.025 ± 0.005 55 n.d. NPG p-Nitrophenyl LecA 14.1 ± 0.2 6.2 — GalAG0 GalA-KPL LecA 4.2 ± 0.9 21 + GalAG1 (GalA-KPL)2KFKI LecA 0.5 ± 0.2 91 ++ GalAG2 33 (GalA-KPL)4(KFKI)2KHI LecA 0.1 ± 0.01 220 +++ GalBG0 GalB-KPL LecA 51.5 ± 6.7 1.7 + GalBG1 (GalB-KPL)2KFKI LecA 2.1 ± 1.0 21 ++
Chem_Soc_Rev_2013_42_4709-4727.docx