(conjugated to AMCA) APAF1 xeroderma pigmentosum, complementation group C; cyclin dependent XPC: p21(CDKN1A): kinase inhibitor 1A; BCL2-associated X protein; apoptotic peptidase activating BAX: APAF1: factor 1. glyceraldehyde-3-phosphate dehydrogenase ; TET: Tetrachloro fluorescein; GAPDH: AMCA: 7-amino-4-methylcoumarin-3-acetic-acid. Movie S1. Time-lapse series of cells demonstrating dual-phase p53 pulses. MCF7-p53 Venus cells were treated with 0.1 Dox. Movie duration: 88 hrs. Images were taken every 30 min. μM Movie S2. Time-lapse series of cells demonstrating single-phase p53 pulses. MCF7-p53 Venus cells were treated with 0.01 Dox. Movie duration: 88 hrs. Images were taken every 30 min. μM Movie S3. Time-lapse series of cells demonstrating sustained p53 accumulation. MCF7-p53 Venus cells were treated with 10 Dox for 8 hrs and cultured after removal of the drug. Movie μM duration: 28 hrs. Images were taken every 30 min.
APAF1 (conjugated to AMCA) XPC: xeroderma pigmentosum, complementation group C; p21(CDKN1A): cyclin dependent kinase inhibitor 1A; BAX: BCL2-associated X protein; APAF1: apoptotic peptidase activating factor 1. GAPDH: glyceraldehyde-3-phosphate dehydrogenase ; TET: Tetrachloro fluorescein; AMCA: 7-amino-4-methylcoumarin-3-acetic-acid. Movie S1. Time-lapse series of cells demonstrating dual-phase p53 pulses. MCF7-p53 Venus cells were treated with 0.1 μM Dox. Movie duration: 88 hrs. Images were taken every 30 min. Movie S2. Time-lapse series of cells demonstrating single-phase p53 pulses. MCF7-p53 Venus cells were treated with 0.01 μM Dox. Movie duration: 88 hrs. Images were taken every 30 min. Movie S3. Time-lapse series of cells demonstrating sustained p53 accumulation. MCF7-p53 Venus cells were treated with 10 μM Dox for 8 hrs and cultured after removal of the drug. Movie duration: 28 hrs. Images were taken every 30 min.
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