Suitable clinical samples should ideally be sent for conventional AFB microscopy and culture investigations as outlined above. Note: It should be noted that if there is not enough specimen volume for PCR and culture, then only culture should be done. All samples, even if PCR positive, should be submitted for culture. 4.5.5 Culture media, conditions and organisms Clinical Specimen Standard media Incubation Cultures Target details/ read organism(s) ‡ conditions Temp Atmos Time oC Sterile Automated liquid If automated monitoring systems are used, after samples: systems decontamination of clinical samples, refer to local protocols and manufacturer’s recommendations. These should be checked continuously for bottles that flag positive. A blood agar plate should be set up for All bone all bottles that are flagged positive to check for contamination. marrow LJ + pyruvate 35 37 Air 8 weeks Weekly – Mycobacterium aspirates extending to 12 species weeks if Wound swab required58 Pus or Pus swabs* CSF Ascites All conditions Joint fluid Pleural fluid Non-sterile Automated liquid If automated monitoring systems are used, after samples: systems decontamination of clinical samples, refer to local Sputum protocols and manufacturer’s recommendations. These should be checked continuously for bottles that flag positive. A blood agar plate should be set up for all bottles that are flagged positive to check for Mycobacterium contamination. tuberculosis washings LJ + pyruvate** 35 37 Air 8 weeks Weekly – Gastric extending to 12 weeks if washings required58 Urine Automated liquid If automated monitoring systems are used, after systems decontamination of clinical samples, refer to local protocols and manufacturer’s recommendations. These should be checked continuously for bottles that flag positive. A blood agar plate should be set up for all bottles that are flagged positive to check for Mycobacterium Genitourinary species contamination. tuberculosis LJ + pyruvate 35 37 Air 8 weeks Weekly – extending to 12 weeks if required58
Suitable clinical samples should ideally be sent for conventional AFB microscopy and culture investigations as outlined above. Note: It should be noted that if there is not enough specimen volume for PCR and culture, then only culture should be done. All samples, even if PCR positive, should be submitted for culture. 4.5.5 Culture media, conditions and organisms Clinical details/ conditions Specimen Standard media Incubation Cultures read Target organism(s) ‡ Temp oC Atmos Time All conditions Sterile samples: All bone marrow aspirates Wound swab Pus or Pus swabs* CSF Ascites Joint fluid Pleural fluid Automated liquid systems If automated monitoring systems are used, after decontamination of clinical samples, refer to local protocols and manufacturer’s recommendations. These should be checked continuously for bottles that flag positive. A blood agar plate should be set up for all bottles that are flagged positive to check for contamination. Mycobacterium species LJ + pyruvate 35 – 37 Air 8 weeks extending to 12 weeks if required58 Weekly Non-sterile samples: Sputum washings Gastric washings Automated liquid systems If automated monitoring systems are used, after decontamination of clinical samples, refer to local protocols and manufacturer’s recommendations. These should be checked continuously for bottles that flag positive. A blood agar plate should be set up for all bottles that are flagged positive to check for contamination. Mycobacterium tuberculosis LJ + pyruvate** 35 – 37 Air 8 weeks extending to 12 weeks if required58 Weekly Genitourinary tuberculosis Urine Automated liquid systems If automated monitoring systems are used, after decontamination of clinical samples, refer to local protocols and manufacturer’s recommendations. These should be checked continuously for bottles that flag positive. A blood agar plate should be set up for all bottles that are flagged positive to check for contamination. Mycobacterium species LJ + pyruvate 35 – 37 Air 8 weeks extending to 12 weeks if required58 Weekly
B_40i7.2.docx