Table S1. Primers for the amplification of catabolic genes alkB, C23O, PAH-RHDα and C12O in P. aeruginosa stain ASP-53 when grown in presence of pyrene. Sl. Expected Gene Primer sequence (Forward) Primer sequence (Reverse) Function References No. Size 5' 5' Incorporation of one oxygen Hesham et al. 1 alkB AACTACMTCGARCAYTACGG 100 bp atom to the substrate and [21] 3' 3' reduction of the other. 5' 5' Oxidative cleavage of catechol 2 C12O GCCAACGTCGACGTCTGGCA 282 bp Sei et al. [18] (intra diol) 3' 3' 5' Oxidative cleavage of catechol 3 C23O 380 bp Sei et al. [18] 3' (extra diol) 3' 5' 5' Creates in aromatic ring by Cebron et al. 4 306 bp oxygenation [20] 3' 3'
Table S1. Primers for the amplification of catabolic genes alkB, C23O, PAH-RHDα and C12O in P. aeruginosa stain ASP-53 when grown in presence of pyrene. Sl. No. Gene Primer sequence (Forward) Primer sequence (Reverse) Expected Size Function References 1 alkB 5' AACTACMTCGARCAYTACGG 3' 5' 3' 100 bp Incorporation of one oxygen atom to the substrate and reduction of the other. Hesham et al. [21] 2 C12O 5' GCCAACGTCGACGTCTGGCA 3' 5' 3' 282 bp Oxidative cleavage of catechol (intra diol) Sei et al. [18] 3 C23O 3' 5' 3' 380 bp Oxidative cleavage of catechol (extra diol) Sei et al. [18] 4 5' 3' 5' 3' 306 bp Creates in aromatic ring by oxygenation Cebron et al. [20]
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