Effective Againsta Tubercle Bacterial bacilli spores viruses viruses Disinfectant Use Parameters Important Characteristics Potential Application cells Alcohol (ethyl, isopropyl) conc.: 70-85% eye irritant, toxic, flammable, surfaces (work & equipment) – + + + ± contact time: 10-30 min. inactivated by organic matter Chlorine Compounds** conc.: 0.05-0.5% (commercial may leave residue; corrosive; skin, spills, equipment surfaces, bleach eye & respiratory irritant; inactivated instruments, glassware, water baths 5%)* + + + + ± contact time: 10-30 min.* by organic matter; make up at least weekly Quaternary Ammonium conc.: 0.1-2% toxic, inactivated by organic matter, surfaces (work & BSCs, floor + + Compounds contact time: 10-30 min. maintenance, glassware, instruments Phenolic Compounds conc.: 0.2-3% leaves residue; corrosive, skin, eye & surfaces (work & equip.), BSCs, + + + contact time: 10-30 min. ± respiratory irritant; inactivated floors, spills, glassware, instruments, by organic matter water baths Iodophor Compounds conc.: 0.47% leaves residue; corrosive, skin & eye surfaces (work & equip.), BSCs, + + contact time: 10-30 min. ± irritant; toxic; inactivated by organic glassware, water baths matter Formaldehydeb (Formalin) conc.: 4-8% leaves residue; skin, eye & respiratory less effective than other disinfectants + + + + contact time: 10-30 min. ± irritant; toxic (carcinogen) but can be used for equipment surfaces, glassware, instruments Glutaraldehyde conc.: 2% leaves residue; skin, eye & respiratory equipment surfaces, glassware, + + + + + contact time: 10-600 min. irritant; toxic instruments
Disinfectant Use Parameters Effective Againsta Important Characteristics Potential Application cells viruses Tubercle bacilli viruses Bacterial spores Alcohol (ethyl, isopropyl) conc.: 70-85% contact time: 10-30 min. + + + ± eye irritant, toxic, flammable, inactivated by organic matter surfaces – (work & equipment) Chlorine Compounds** conc.: 0.05-0.5% (commercial bleach 5%)* contact time: 10-30 min.* + + + + ± may leave residue; corrosive; skin, eye & respiratory irritant; inactivated by organic matter; make up at least weekly spills, equipment surfaces, instruments, glassware, water baths Quaternary Ammonium Compounds conc.: 0.1-2% contact time: 10-30 min. + + toxic, inactivated by organic matter, surfaces (work & BSCs, floor maintenance, glassware, instruments Phenolic Compounds conc.: 0.2-3% contact time: 10-30 min. + + + ± leaves residue; corrosive, skin, eye & respiratory irritant; inactivated by organic matter surfaces (work & equip.), BSCs, floors, spills, glassware, instruments, water baths Iodophor Compounds conc.: 0.47% contact time: 10-30 min. + + ± leaves residue; corrosive, skin & eye irritant; toxic; inactivated by organic matter surfaces (work & equip.), BSCs, glassware, water baths Formaldehydeb (Formalin) conc.: 4-8% contact time: 10-30 min. + + + + ± leaves residue; skin, eye & respiratory irritant; toxic (carcinogen) less effective than other disinfectants but can be used for equipment surfaces, glassware, instruments Glutaraldehyde conc.: 2% contact time: 10-600 min. + + + + + leaves residue; skin, eye & respiratory irritant; toxic equipment surfaces, glassware, instruments
Biosafety_Manual-May2009.docx