Detection ARGs Sequence (5’-3’) Thermal profile Cycles Reference format 95 °C 3 min Fierer et al. 95 °C 15 s, 60 °C 40 SYBR Green (2005) 45 s 95 °C 3 min Barraud et Intl1 95 °C 15 s, 60 °C 40 TaqMan al. (2010) 45 s 95 °C 3 min Knapp et al. ermB 95 °C 15 s, 60 °C 40 SYBR Green (2010) 45 s 95 °C 3 min Heuer and ccgttggccttcctgtaaag sul1 95 °C 15 s, 60 °C 40 TaqMan Smalla ttgccgatcgcgtgaagt 45 s (2007) 95 °C 3 min cggctgcgcttcgatt Heuer et al. sul2 95 °C 15 s, 60 °C 40 TaqMan cgcgcgcagaaaggatt (2008) 45 s 95 °C 3 min Walsh et al. tetW 45 TaqMan 95 °C 15 s, 59 °C (2011) Text S1. Indicatory parameters of WWTP influence.
ARGs Sequence (5’-3’) Thermal profile Cycles Detection format Reference 95 °C 3 min 95 °C 15 s, 60 °C 45 s 40 SYBR Green Fierer et al. (2005) Intl1 95 °C 3 min 95 °C 15 s, 60 °C 45 s 40 TaqMan Barraud et al. (2010) ermB 95 °C 3 min 95 °C 15 s, 60 °C 45 s 40 SYBR Green Knapp et al. (2010) sul1 ccgttggccttcctgtaaag ttgccgatcgcgtgaagt 95 °C 3 min 95 °C 15 s, 60 °C 45 s 40 TaqMan Heuer and Smalla (2007) sul2 cggctgcgcttcgatt cgcgcgcagaaaggatt 95 °C 3 min 95 °C 15 s, 60 °C 45 s 40 TaqMan Heuer et al. (2008) tetW 95 °C 3 min 95 °C 15 s, 59 °C 45 TaqMan Walsh et al. (2011) Text S1. Indicatory parameters of WWTP influence.
1-s2.0-S2213343718301258-mmc1.docx