Wipe down readily accessible interior surfaces with 70% ethanol (b) Shut down Decontaminate items with 70% ethanol and remove from the interior work area Wipe down the interior surfaces with disinfectant followed by 70% ethanol Allow 5 minutes of operation to purge the system Turn off cabinet blower Lower the cabinet sash and turn on the UV light Spill Procedures within a BSC Keep the cabinet blower on Replace gloves if they are contaminated Cover the spill with paper towels to prevent aerosols and splashing a fresh solution of disinfectant to the area Wait out the prescribed time for the materials involved and the disinfectant used Wipe down any equipment in the cabinet with paper towels dampened with disinfectant Collect the paper towels with more paper towels, working from the outside in; use tongs if sharps are involved Bag the materials as solid waste Remove gloves aseptically and wash your thoroughly BSCs are not the same as Chemical Fume or Laminar Flow Hoods Do not use volatile chemicals in recirculating class II BSCs. Be aware some chemicals may damage the HEPA filtration system. Use a fume hood for volatile chemicals. Type of Protection II type A2 BSC Chemical Fume Laminar Flow Hood Hood Personnel Yes Yes No Product Yes No Yes Environment Yes No No Certification and Servicing Biological safety cabinets must be tested and certified for proper function after installation, relocation, or maintenance, and at least annually. The University of California has negotiated a system-wide agreement with Technical Safety Services, Inc., outlining service expectations and pricing schedules. The Los Angeles branch serves UCSB and a field manager may be reached at 562-694-3626 or 800-877-7742. Lead times vary, but technicians can usually visit campus within a week.
Wipe down readily accessible interior surfaces with 70% ethanol (b) Shut down Decontaminate items with 70% ethanol and remove from the interior work area Wipe down the interior surfaces with disinfectant followed by 70% ethanol Allow 5 minutes of operation to purge the system Turn off cabinet blower Lower the cabinet sash and turn on the UV light Spill Procedures within a BSC Keep the cabinet blower on Replace gloves if they are contaminated Cover the spill with paper towels to prevent aerosols and splashing a fresh solution of disinfectant to the area Wait out the prescribed time for the materials involved and the disinfectant used Wipe down any equipment in the cabinet with paper towels dampened with disinfectant Collect the paper towels with more paper towels, working from the outside in; use tongs if sharps are involved Bag the materials as solid waste Remove gloves aseptically and wash your thoroughly BSCs are not the same as Chemical Fume or Laminar Flow Hoods Do not use volatile chemicals in recirculating class II BSCs. Be aware some chemicals may damage the HEPA filtration system. Use a fume hood for volatile chemicals. Type of Protection II type A2 BSC Chemical Fume Hood Laminar Flow Hood Personnel Yes Yes No Product Yes No Yes Environment Yes No No Certification and Servicing Biological safety cabinets must be tested and certified for proper function after installation, relocation, or maintenance, and at least annually. The University of California has negotiated a system-wide agreement with Technical Safety Services, Inc., outlining service expectations and pricing schedules. The Los Angeles branch serves UCSB and a field manager may be reached at 562-694-3626 or 800-877-7742. Lead times vary, but technicians can usually visit campus within a week.
BSC_Use.docx