Appendix A Overview of Illumina sequence data (V9 region of the SSU rRNA) after sequencing, quality control and taxonomic assignment. “Total” in column refers to the sum of all replicates from the corresponding sampling site. Please note that the total number of “Replicate” OTUs obtained per sampling site is the number of distinct OTUs and not the sum of all OTUs from the replicates/site. Number of total Number of high-quality Number of protistan Distance from cage [m] Replicate sequences obtained protistan sequences OTUs 1 42,454 39,018 565 0 2 40,175 37,857 618 3 52,897 50,933 698 total 135,526 127,808 954 1 43,022 41,368 696 26 2 47,382 44,192 691 3 51,743 47,042 635 total 142,147 132,602 1,132 1 42,389 40,052 835 60 2 45,327 42,779 841 3 40,928 38,829 691 total 128,644 121,660 1,298 1 47,128 44,545 1,184 270 2 43,752 41,548 985 3 49,344 46,735 1,233 total 140,224 132,828 1,799 1 52,294 49,466 893 400 2 53,518 50,735 906 3 50,721 47,835 922 total 156,533 148,036 1,472
Appendix A Overview of Illumina sequence data (V9 region of the SSU rRNA) after sequencing, quality control and taxonomic assignment. “Total” in column “Replicate” refers to the sum of all replicates from the corresponding sampling site. Please note that the total number of OTUs obtained per sampling site is the number of distinct OTUs and not the sum of all OTUs from the replicates/site. Distance from cage [m] Replicate Number of total sequences obtained Number of high-quality protistan sequences Number of protistan OTUs 0 1 42,454 39,018 565 2 40,175 37,857 618 3 52,897 50,933 698 total 135,526 127,808 954 26 1 43,022 41,368 696 2 47,382 44,192 691 3 51,743 47,042 635 total 142,147 132,602 1,132 60 1 42,389 40,052 835 2 45,327 42,779 841 3 40,928 38,829 691 total 128,644 121,660 1,298 270 1 47,128 44,545 1,184 2 43,752 41,548 985 3 49,344 46,735 1,233 total 140,224 132,828 1,799 400 1 52,294 49,466 893 2 53,518 50,735 906 3 50,721 47,835 922 total 156,533 148,036 1,472
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