The decolorizing process was recorded after 5 min Molecular identification of the bacterial isolates of reaction at 517 nm and compared with a blank Molecular characterization of the two control. bacterial strains was carried out by PCR Antioxidant activity (DPPH scavenging effect %) = amplification of 16S rDNA gene, a fraction of the ((A0- A1)/ A0) x 100 PCR mixture was examined using agarose gel Where, A0 was the absorbance of the control and A1 electrophoresis. According to sequencing similarities was the absorbance in the presence of the bacterial and multiple alignment, the bacterial strain isolated crude extract. from were found to be in Acanthophora dendroides a close relation to (ac: Bacterium SRCnm 3. Results and Discussion GQ979939.) and these isolated from Sarggassum Identification of the seaweeds was similar to sabrepandum Bacillus sp. JS,(ac: The seaweeds identified as CP003492), with 100% identity. The phylogenetic Acanthophora (red algae) and tree of was displayed using the dendroides Sarggassum Bacterium SRCnm (brown algae) by Biological TREEVIEW program (Fig 1). sabrepandum researchers, National Institute of Oceanography and Fisheries, Egypt. Fig. 1: The phylogenetic tree based on PCR product sequencing of DNA isolated from the endophytic Bacterium SRCnm. In accordance with our results, endobiotic (Cla´udia 2010 & Jamal and Mudarris sp. et al., was previously isolated from 2010). Furthermore, Sutha (2011) isolated Bacillus sp. Sargassum et al.
The decolorizing process was recorded after 5 min of reaction at 517 nm and compared with a blank control. Antioxidant activity (DPPH scavenging effect %) = ((A0- A1)/ A0) x 100 Where, A0 was the absorbance of the control and A1 was the absorbance in the presence of the bacterial crude extract. 3. Results and Discussion Identification of the seaweeds The seaweeds identified as Acanthophora dendroides (red algae) and Sarggassum sabrepandum (brown algae) by Biological researchers, National Institute of Oceanography and Fisheries, Egypt. Molecular identification of the bacterial isolates Molecular characterization of the two bacterial strains was carried out by PCR amplification of 16S rDNA gene, a fraction of the PCR mixture was examined using agarose gel electrophoresis. According to sequencing similarities and multiple alignment, the bacterial strain isolated from Acanthophora dendroides were found to be in a close relation to Bacterium SRCnm (ac: GQ979939.) and these isolated from Sarggassum sabrepandum was similar to Bacillus sp. JS,(ac: CP003492), with 100% identity. The phylogenetic tree of Bacterium SRCnm was displayed using the TREEVIEW program (Fig 1). Fig. 1: The phylogenetic tree based on PCR product sequencing of DNA isolated from the endophytic Bacterium SRCnm. In accordance with our results, endobiotic Bacillus sp. was previously isolated from Sargassum sp. (Cla´udia et al., 2010 & Jamal and Mudarris 2010). Furthermore, Sutha et al. (2011) isolated
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