BOT E14 Elective Theory ‘A’ Cytogenetics and Crop Improvement Credits: 05 Full Marks: 70 Time: 03 Hrs In all nine questions of equal value will be set, out of which a student shall have to answer five questions. Q.no. 1 will be compulsory, consisting of seven very short answer type questions (each of two marks) covering the entire syllabus and the candidates are required to give their answers in maximum 50 words. : Plant Tissue Culture Somatic embryogenesis: Introduction; sources for somatic embryogenesis; direct and indirect embryogenesis; protocol and the factors affecting somatic embryogenesis; significance; the artificial seeds. Micropropagation : Introduction; protocol; sources; advantages. Protoplast culture and Somatic hybridization: Objectives; protocol; selection of the fused protoplasts; chromosome status of the somatic hybrids; significance; cybrids. Anther and Pollen culture: Objectives; protocols for anther and pollen culture; comparison between anther and pollen culture; screening of haploids; methods of diploidization; significance of anther and pollen culture. : Recombinant DNA Technology Tools of genetic engineering: The cloning vectors - plasmids, cosmids, phages, BAC and YAC vectors; restriction endonucleases; ligases; gel electrophoresis; probes; Southern, Northern and Western blottings.
BOT E14 Elective Theory ‘A’ Cytogenetics and Crop Improvement Credits: 05 Full Marks: 70 Time: 03 Hrs In all nine questions of equal value will be set, out of which a student shall have to answer five questions. Q.no. 1 will be compulsory, consisting of seven very short answer type questions (each of two marks) covering the entire syllabus and the candidates are required to give their answers in maximum 50 words. : Plant Tissue Culture Somatic embryogenesis: Introduction; sources for somatic embryogenesis; direct and indirect embryogenesis; protocol and the factors affecting somatic embryogenesis; significance; the artificial seeds. Micropropagation : Introduction; protocol; sources; advantages. Protoplast culture and Somatic hybridization: Objectives; protocol; selection of the fused protoplasts; chromosome status of the somatic hybrids; significance; cybrids. Anther and Pollen culture: Objectives; protocols for anther and pollen culture; comparison between anther and pollen culture; screening of haploids; methods of diploidization; significance of anther and pollen culture. : Recombinant DNA Technology Tools of genetic engineering: The cloning vectors - plasmids, cosmids, phages, BAC and YAC vectors; restriction endonucleases; ligases; gel electrophoresis; probes; Southern, Northern and Western blottings.
Botany1.docx