S1. Specific primer sets used for Gnomoniopsis castanea molecular identification Annealing Primer Gene Primer sequence Reference name Carbone and Kohn, EF1-728F 5’-CATCGAGAAGTTCGAGAAGG-3’ Elongation 1999 58 °C factor 1-α EF1-1199R 5-’GGGAAGTACCMGTGATCATGT-3’ Walker et al., 2010 5F GGGGWGAYCAGAAGAAGGC RNA Liu, Whelen, & Hall 50 °C polymerase II (1999) 7CR CCCATRGCTTGYTTRCCCAT T1 O’Donnell and Cigelnik, 56 °C β-tubulin 1997 T2 5’-AACATGCGTGAGATTGTAAGT-3’ ITS1 5’-TCCGTAGGTGAACCTGCGG-3’ ITS 55 °C White et al., 1990 ITS4 5’-TCCTCCGCTTATTGATATGC-3’
S1. Specific primer sets used for Gnomoniopsis castanea molecular identification Gene Primer name Primer sequence Annealing Reference Elongation factor 1-α EF1-728F 5’-CATCGAGAAGTTCGAGAAGG-3’ 58 °C Carbone and Kohn, 1999 EF1-1199R 5-’GGGAAGTACCMGTGATCATGT-3’ Walker et al., 2010 RNA polymerase II 5F GGGGWGAYCAGAAGAAGGC 50 °C Liu, Whelen, & Hall (1999) 7CR CCCATRGCTTGYTTRCCCAT β-tubulin T1 56 °C O’Donnell and Cigelnik, 1997 T2 5’-AACATGCGTGAGATTGTAAGT-3’ ITS ITS1 5’-TCCGTAGGTGAACCTGCGG-3’ 55 °C White et al., 1990 ITS4 5’-TCCTCCGCTTATTGATATGC-3’
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