Biological Safety Cabinets used for infectious / It’s okay to pipette tips and potentially infectious material must be certified microcentrifuge tubes in the biological safety at least: cabinet as long as they are pushed up against the back wall of the cabinet. Daily True Monthly False Yearly Once Which of the following practices should be utilized when working in a biological safety Which of the following protect both the user cabinet? and the material that is handled inside the cabinet Disinfect all surfaces of the BSC before and work Fume hood Disinfect all items which go into and come Clean bench out of the BSC Biological safety cabinet (BSC) Allow the BSC to operate before work All of the begins and after work ceases All of the above 30. In a II, Type A2 biological safety cabinet (typical unit found in most labs): a spill should occur within a biological Downward laminar flow splits 2-6 inches safety cabinet (BSC), what steps would above work surface between front and immediately be taken? rear grills A. Turn off the BSC, grab the biological HEPA-filtered air from the cabinet is in the unit and also exhausts to spill kit, and immediately wipe up the spill the room with a dry, absorbent towel It is safe to work with substantial amounts Turn off the BSC and pour a germicidal of chemicals and radiolabeled nucleotides agent on the spill A and B only Turn off the BSC, evacuate the laboratory, and notify company safety authorities 31. Open flames are not allowed in a Leave the BSC on, grab the biological spill biological safety cabinet because kit, add absorbent material and germicidal agent, wipe up the spill once contact time They disrupt airflow patterns that protect has permitted inactivation, and remove user and product corrosive residue with 70% ethanol. They can damage HEPA filters Leave the BSC on, grab the biological spill They pose a fire hazard kit and wipe up the spill with a dry, All of the above absorbent towel Acceptable alternatives to Bunsen burner use 36. Disinfectants decontaminate soiled and in the biological safety cabinet (BSC) include: clean surfaces equally well. Disposable loops, spreaders, or glass True beads False Bacticinerator Touch-O-Matic burner All of the above
Biological Safety Cabinets used for infectious / potentially infectious material must be certified at least: Daily Monthly Yearly Once Which of the following protect both the user and the material that is handled inside the cabinet Fume hood Clean bench Biological safety cabinet (BSC) All of the 30. In a II, Type A2 biological safety cabinet (typical unit found in most labs): Downward laminar flow splits 2-6 inches above work surface between front and rear grills HEPA-filtered air from the cabinet is in the unit and also exhausts to the room It is safe to work with substantial amounts of chemicals and radiolabeled nucleotides A and B only 31. Open flames are not allowed in a biological safety cabinet because They disrupt airflow patterns that protect user and product They can damage HEPA filters They pose a fire hazard All of the above Acceptable alternatives to Bunsen burner use in the biological safety cabinet (BSC) include: Disposable loops, spreaders, or glass beads Bacticinerator Touch-O-Matic burner All of the above It’s okay to pipette tips and microcentrifuge tubes in the biological safety cabinet as long as they are pushed up against the back wall of the cabinet. True False Which of the following practices should be utilized when working in a biological safety cabinet? Disinfect all surfaces of the BSC before and work Disinfect all items which go into and come out of the BSC Allow the BSC to operate before work begins and after work ceases All of the above a spill should occur within a biological safety cabinet (BSC), what steps would immediately be taken? A. Turn off the BSC, grab the biological spill kit, and immediately wipe up the spill with a dry, absorbent towel Turn off the BSC and pour a germicidal agent on the spill Turn off the BSC, evacuate the laboratory, and notify company safety authorities Leave the BSC on, grab the biological spill kit, add absorbent material and germicidal agent, wipe up the spill once contact time has permitted inactivation, and remove corrosive residue with 70% ethanol. Leave the BSC on, grab the biological spill kit and wipe up the spill with a dry, absorbent towel 36. Disinfectants decontaminate soiled and clean surfaces equally well. True False
Biosafety_Quiz.docx