A partial validation must assess the recovery, precision and accuracy of the method across the expected concentration range and determine the method limits (LOD and LLOQ) in the secondary matrix, characterize matrix effects on instrument response and/or recovery for the secondary matrix, and establish the blank response for the chemical or test article in the secondary matrix. The partial validation is run on the secondary matrix(ces) the procedures given in section 4.2.2.5.4.4. Partial Validation Procedure, below. Partial Validation Procedure Solvent Standards Preparation Prepare solvent standards with the same final concentrations (i.e., after any extraction, dilution, or concentration step) as the primary matrix standards. Single replicates of each solvent standard shall be prepared from one stock solution. Use the same standard stock solution (Stock A or Stock B) prepared for the full validation, or a freshly prepared stock at the same concentration, to prepare the solvent standards. Measure the solvent standard responses using the same analytical system used for the full validation in the primary matrix. Matrix Standards Preparation Sample preparation methods are typically based on those used for the primary matrix, but may deviate as
A partial validation must assess the recovery, precision and accuracy of the method across the expected concentration range and determine the method limits (LOD and LLOQ) in the secondary matrix, characterize matrix effects on instrument response and/or recovery for the secondary matrix, and establish the blank response for the chemical or test article in the secondary matrix. The partial validation is run on the secondary matrix(ces) the procedures given in section 4.2.2.5.4.4. Partial Validation Procedure, below. Partial Validation Procedure Solvent Standards Preparation Prepare solvent standards with the same final concentrations (i.e., after any extraction, dilution, or concentration step) as the primary matrix standards. Single replicates of each solvent standard shall be prepared from one stock solution. Use the same standard stock solution (Stock A or Stock B) prepared for the full validation, or a freshly prepared stock at the same concentration, to prepare the solvent standards. Measure the solvent standard responses using the same analytical system used for the full validation in the primary matrix. Matrix Standards Preparation Sample preparation methods are typically based on those used for the primary matrix, but may deviate as
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