Human blood dilutions Dilution factor 1 101 102 103 104 105 106 +++ +++ ++ ++ + + - Combur3 Test® E Phenolphthalein DischapsTM +++ ++ + - - - - Leuco-Malachite DischapsTM +++ +++ ++ + + - - Hemastix® +++ +++ + + + + - Notes: See Table A1 notes for results interpretation. Table A3. Quantification of Combur3 Test® E exposed and unexposed bloodstains. Combur3 Test® Mean DNA quantification results ([DNA]) E exposure Human blood dilutions Dilution factor 1 101 102 103 104 105 106 Combur3 Test® [DNA]≥LoD [DNA]<LoD [DNA] U [DNA] [DNA] U E exposed IC≤30 Ct IC≤30 Ct IC≤30 U IC≤30 Ct IC≤30 Ct IC≤30 Ct Ct IC≤30 bloodstains Ct Combur3 Test® [DNA]≥ [DNA]≥ [DNA]≥ LoD [DNA]≥ [DNA]< [DNA] [DNA] U E unexposed LoD LoD IC≤30 Ct LoD LoD U IC≤30 Ct bloodstains IC≤30 Ct IC≤30 Ct IC≤30 Ct IC≤30 IC≤30 Ct Ct Diluted bloodstains on simulated evidences Dilution factor 103 104 105 Combur3 Test® [DNA]≥ [DNA]< [DNA] E exposed LoD LoD U bloodstains IC≤30 Ct IC≤30 IC≤30 Ct Ct Combur3 Test® [DNA]≥ [DNA]< [DNA] E unexposed LoD LoD U bloodstains IC≤30 Ct IC≤30 IC≤30 Ct Ct Human bloodstains on evidences from real caseworks (undiluted, always visible to the eye) Combur3 Test® [DNA]≥LoD E exposed IC≤30 Ct bloodstains
Human blood dilutions Dilution factor 1 101 102 103 104 105 106 Combur3 Test® E +++ +++ ++ ++ + + - Phenolphthalein DischapsTM +++ ++ + - - - - Leuco-Malachite DischapsTM +++ +++ ++ + + - - Hemastix® +++ +++ + + + + - Notes: See Table A1 notes for results interpretation. Table A3. Quantification of Combur3 Test® E exposed and unexposed bloodstains. Combur3 Test® E exposure Mean DNA quantification results ([DNA]) Human blood dilutions Dilution factor 1 101 102 103 104 105 106 Combur3 Test® E exposed bloodstains IC≤30 Ct IC≤30 Ct [DNA]≥LoD IC≤30 Ct [DNA]<LoD IC≤30 Ct [DNA] U IC≤30 Ct [DNA] U IC≤30 Ct [DNA] U IC≤30 Ct Combur3 Test® E unexposed bloodstains [DNA]≥ LoD IC≤30 Ct [DNA]≥ LoD IC≤30 Ct [DNA]≥ LoD IC≤30 Ct [DNA]≥ LoD IC≤30 Ct [DNA]< LoD IC≤30 Ct [DNA] U IC≤30 Ct [DNA] U IC≤30 Ct Diluted bloodstains on simulated evidences Dilution factor 103 104 105 Combur3 Test® E exposed bloodstains [DNA]≥ LoD IC≤30 Ct [DNA]< LoD IC≤30 Ct [DNA] U IC≤30 Ct Combur3 Test® E unexposed bloodstains [DNA]≥ LoD IC≤30 Ct [DNA]< LoD IC≤30 Ct [DNA] U IC≤30 Ct Human bloodstains on evidences from real caseworks (undiluted, always visible to the eye) Combur3 Test® E exposed bloodstains [DNA]≥LoD IC≤30 Ct
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