are collected from the lysed cells and are plated on bacteria. The numbers of different progeny phages are given here. a. ) Determine the of the three genes on the phage chromosome. Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 b.) Determine the map distance between the h+ c st 26 genes. h c+ st+ 30 h+ c st+ 106 h c+ st 110 Two mutations that affect plaque h+ c+ st 5 morphology in phages (a- and b-) have been isolated. Phages carrying both mutations (a- h c st+ 6 and b-) are mixed with wild-type phages (a+ and b+) and added to a culture of bacterial cells. Subsequent to infection and lysis, samples of the phage lysate are collected and cultured on bacterial cells. The following numbers of plaques are observed: What is the frequency of recombination between the a and b genes? Plaque phenotype Number a+ b+ 2043 geneticist isolates two bacteriophage r mutants (r13 and r2) that cause rapid lysis. He carries out the following crosses and a+ b- 320 counts the number of plaques listed here: a- b+ 357 a- b- 2134 Genotype of Parental Phage Progeny Number h+ r13- x h- r13+ h+ r13+ 1 h- r13+ 104 h+ r13- 110 h- r13- 2 h+ r2- x h- r2+ h+ r2+ 6 h- r2+ 86 h+ r2- 81 h- r2- 7 Calculate the recombination frequencies between r2 and h and between r13 and h. Draw all possible linage maps for three genes.
are collected from the lysed cells and are plated on bacteria. The numbers of different progeny phages are given here. a. ) Determine the of the three genes on the phage chromosome. b.) Determine the map distance between the genes. Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 Two mutations that affect plaque morphology in phages (a- and b-) have been isolated. Phages carrying both mutations (a- and b-) are mixed with wild-type phages (a+ and b+) and added to a culture of bacterial cells. Subsequent to infection and lysis, samples of the phage lysate are collected and cultured on bacterial cells. The following numbers of plaques are observed: Plaque phenotype Number a+ b+ 2043 a+ b- 320 a- b+ 357 a- b- 2134 What is the frequency of recombination between the a and b genes? geneticist isolates two bacteriophage r mutants (r13 and r2) that cause rapid lysis. He carries out the following crosses and counts the number of plaques listed here: Genotype of Parental Phage Progeny Number h+ r13- x h- r13+ h+ r13+ 1 h- r13+ 104 h+ r13- 110 h- r13- 2 h+ r2- x h- r2+ h+ r2+ 6 h- r2+ 86 h+ r2- 81 h- r2- 7 Calculate the recombination frequencies between r2 and h and between r13 and h. Draw all possible linage maps for three genes.
BIOL_GEN_313_Myers___Vollbrecht_trt_SI_032416.docx