Fig. S3. CORM-3 inhibits cell growth and is bactericidal to MG1655 cell cultures E. coli grown on glucose defined minimal medium (GDMM). (a) Cell growth, assessed by monitoring culture optical density (OD600), in the absence of CORM-3 (closed circles) or after the addition of 15 (dashed line, closed squares), 30 (open squares), 60 μM μM μM (dashed line, closed triangles) or 120 (open triangles) CORM-3. Growth was completely μM inhibited at 30 CORM-3. (b) Culture viability, assessed via colony forming units per ≥ μM mL (CFU mL-1), in the absence of CORM-3 (black bars) or after the addition of 15 μM (white bars), 30 (light grey bars), 60 (dark grey bars) or 120 (black/grey bars) μM μM μM CORM-3. Arrows indicate where the culture viability was undetectable. CORM-3 caused a dose-dependent increase in cell killing, with a complete loss in culture viability at 120 min at 60 CORM-3. Data represent 3 biological repeats ± SD. ≥ μM Supplementary Data Fig. S4
Fig. S3. CORM-3 inhibits cell growth and is bactericidal to E. coli MG1655 cell cultures grown on glucose defined minimal medium (GDMM). (a) Cell growth, assessed by monitoring culture optical density (OD600), in the absence of CORM-3 (closed circles) or after the addition of 15 μM (dashed line, closed squares), 30 μM (open squares), 60 μM (dashed line, closed triangles) or 120 μM (open triangles) CORM-3. Growth was completely inhibited at ≥ 30 μM CORM-3. (b) Culture viability, assessed via colony forming units per mL (CFU mL-1), in the absence of CORM-3 (black bars) or after the addition of 15 μM (white bars), 30 μM (light grey bars), 60 μM (dark grey bars) or 120 μM (black/grey bars) CORM-3. Arrows indicate where the culture viability was undetectable. CORM-3 caused a dose-dependent increase in cell killing, with a complete loss in culture viability at 120 min at ≥ 60 μM CORM-3. Data represent 3 biological repeats ± SD. Supplementary Data Fig. S4
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