14 25, 27 Bioinformatics NCBI website 15 May 2, 4 Next-Generation Sequencing Lecture and lab weeks 13-15; specific topics from May 11th Final Exam 7:15 am 9:15 pm – throughout the semester as designated by the instructor ***** You are expected to arrive prepared for laboratory work designated by the instructor during the previous Absolute preparation includes: Lab notebook Necessary protocols or SOPS printed out and pages inserted into notebook Black permanent pen Lab coat Safety goggles or glasses Calculator Tentative laboratory schedule Week Date Activity Reading 1 Jan. 24, 26 Laboratory introduction; lab safety; micropipette HO - SOP hand washing use ppt; lab math review; keeping a lab notebook and gloving; micropipette ppt; begin micropipette use; Micropipette validation SOP; pH meter validation exercise use SOP; SOP for 1M pH 8; HO media – prep SOP; pouring plates SOP; SOP for Prep of 10x TBE 2 31, Feb.2 Begin solution preparation; use of pH meter: HO transformation of E. – calibration exercise and prep of 1 M Tris-HCl, pH SOP; spreading plates coli SOP; picking single
14 25, 27 Bioinformatics NCBI website 15 May 2, 4 Next-Generation Sequencing May 11th Final Exam 7:15 am – 9:15 pm Lecture and lab weeks 13-15; specific topics from throughout the semester as designated by the instructor ***** You are expected to arrive prepared for laboratory work designated by the instructor during the previous Absolute preparation includes: Lab notebook Necessary protocols or SOPS printed out and pages inserted into notebook Black permanent pen Lab coat Safety goggles or glasses Calculator Tentative laboratory schedule Week Date Activity Reading 1 Jan. 24, 26 Laboratory introduction; lab safety; micropipette use ppt; lab math review; keeping a lab notebook ppt; begin micropipette use; Micropipette validation exercise HO - SOP hand washing and gloving; micropipette validation SOP; pH meter use SOP; SOP for 1M pH 8; HO – media prep SOP; pouring plates SOP; SOP for Prep of 10x TBE 2 31, Feb.2 Begin solution preparation; use of pH meter: calibration exercise and prep of 1 M Tris-HCl, pH HO – transformation of E. coli SOP; spreading plates SOP; picking single
BIOT%20240%20-%20Nucleic%20Acid%20Methods.docx