intestines. Blue indicates DAPI staining for DNA. Scale bars: 20 μm. Fig. S5. Renal tumors induced by expression are inhibited by autophagy Rafgof induction. (A) Control renal clones grown in well-fed flies (rich medium) at 29 ºC for 3 days AY (white arrowheads). (B) Renal tumors are formed in well-fed animals after AY induction of at 29 ºC for 3 days (white arrowheads). (C) renal clones grown Rafgof AY in well-fed flies expressing at 29 ºC for 3 days (white arrowheads). (D) Renal Atg1 tumors are significantly inhibited by co-expressing (white arrowhead). Rafgof Atg1 (E) Renal tumors are significantly inhibited under starvation (white Rafgof arrowheads). Blue indicates DAPI staining for DNA. Scale bars: 20 μm. Fig. S6. Autophagy is required for the inhibition of tumors. Rafgof (A) Intestinal tumors are formed in intestines at 29°C for 3 days (white esgts>Rafgof arrowheads). (B) No obvious defects are observed in intestines (white esgts>Atg1 arrowheads). (C) Intestines with knockdown of (progenitors in green, white Atg5 arrowheads). (D) Intestinal tumors are formed in intestines at esgts>Atg5RNAi, Rafgof 29°C for 3 days (white arrowheads). (E) RNAi significantly rescues the Atg5 inhibition of tumor formation observed in intestines (white esgts>Atg1, Rafgof arrowheads). (F) Intestines with knockdown of (progenitors in green, white Atg6 arrowheads). (G) Intestinal tumors are formed in intestines at esgts>Atg6RNAi, Rafgof 29°C for 3 days (white arrowheads). (H) knockdown significantly rescues the Atg6 inhibition of tumor formation observed in intestines (white esgts>Atg1, Rafgof arrowheads). (I) Quantification of the relative number of cells in the intestines of esg+
intestines. Blue indicates DAPI staining for DNA. Scale bars: 20 μm. Fig. S5. Renal tumors induced by Rafgof expression are inhibited by autophagy induction. (A) Control AY renal clones grown in well-fed flies (rich medium) at 29 ºC for 3 days (white arrowheads). (B) Renal tumors are formed in well-fed animals after AY induction of Rafgof at 29 ºC for 3 days (white arrowheads). (C) AY renal clones grown in well-fed flies expressing Atg1 at 29 ºC for 3 days (white arrowheads). (D) Renal Rafgof tumors are significantly inhibited by co-expressing Atg1 (white arrowhead). (E) Renal Rafgof tumors are significantly inhibited under starvation (white arrowheads). Blue indicates DAPI staining for DNA. Scale bars: 20 μm. Fig. S6. Autophagy is required for the inhibition of Rafgof tumors. (A) Intestinal tumors are formed in esgts>Rafgof intestines at 29°C for 3 days (white arrowheads). (B) No obvious defects are observed in esgts>Atg1 intestines (white arrowheads). (C) Intestines with knockdown of Atg5 (progenitors in green, white arrowheads). (D) Intestinal tumors are formed in esgts>Atg5RNAi, Rafgof intestines at 29°C for 3 days (white arrowheads). (E) Atg5 RNAi significantly rescues the inhibition of tumor formation observed in esgts>Atg1, Rafgof intestines (white arrowheads). (F) Intestines with knockdown of Atg6 (progenitors in green, white arrowheads). (G) Intestinal tumors are formed in esgts>Atg6RNAi, Rafgof intestines at 29°C for 3 days (white arrowheads). (H) Atg6 knockdown significantly rescues the inhibition of tumor formation observed in esgts>Atg1, Rafgof intestines (white arrowheads). (I) Quantification of the relative number of esg+ cells in the intestines of
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