Protocol/Procedure for Bromine Preparation List any other particular preparation requirements needs for this procedure (e.g., location of spill kit or keep water or ignition sources away from procedure area). Know the location of the nearest fire extinguisher, eyewash, and safety shower before beginning work. Care should be when handling bromine to avoid reducing agents due to a potentially violent reaction. Lab-specific Add lab-specific information not included above if needed (e.g., all work for this procedure is to take place in the Information designated fume hood.) All work with bromine should be carried out in a functioning fume hood reactions are complete, it is recommended that any excess bromine is quenched with a mild reducing agent solution, such a sodium thiosulfate before workup Procedure/Use Scale Engineering PPE (eye, face, gloves, Procedure Steps and Precautions Controls/Equipment clothing) 1. Bromine is It may be All work using Bromine Wear Eye protection: Bromine is very hazardous upon used in the lab as used in must be performed in a safety goggles and face skin contact. Special Remarks on a reagent in quantities ventilated fume hood. shield when working with liquid Explosion Hazards: chemical ranging bromine. reactions. from <1mL Eliminate ignition REACTS EXPLOSIVELY WITH up to 10 sources such as open Face protection: Wear a face ACETYLENE, ACRYLONITRILE, mL. flames, hot surfaces, shield when handling AMMONIA, DIMETHYL steam baths, static containers of Bromine FORMAMIDE, ETHYL Variations electricity, and operation PHOSPHINE, HYDROGEN, must be of mechanical and Use or Nitrile Gloves: ISOBUTYROPHENONE, NICKEL approved electrical equipment that Gloves when working with CARBONYL, NITROGEN by the PI. is not intrinsically safe. bromine water. Work with TRIIODIDE, OZONE, OXYGEN liquid bromine requires DIFLUORIDE, PHOSPHORUS, Ensure proper grounding fluorinated rubber gloves. POTASSIUM, SILVER AZIDE, and avoid creating static SODIUM, & SODIUM CARBIDE. electricity. Be sure to Clothing: Long closed-toed ground metal containers and closed-heeled shoes, when transferring Use in a fume hood and cap clothing/attire, and flammable liquids. container when not in use.
Protocol/Procedure for Bromine Preparation List any other particular preparation requirements needs for this procedure (e.g., location of spill kit or keep water or ignition sources away from procedure area). Know the location of the nearest fire extinguisher, eyewash, and safety shower before beginning work. Care should be when handling bromine to avoid reducing agents due to a potentially violent reaction. Lab-specific Information Add lab-specific information not included above if needed (e.g., all work for this procedure is to take place in the designated fume hood.) All work with bromine should be carried out in a functioning fume hood reactions are complete, it is recommended that any excess bromine is quenched with a mild reducing agent solution, such a sodium thiosulfate before workup Procedure/Use Scale Engineering Controls/Equipment PPE (eye, face, gloves, clothing) Procedure Steps and Precautions 1. Bromine is used in the lab as a reagent in chemical reactions. It may be used in quantities ranging from <1mL up to 10 mL. Variations must be approved by the PI. All work using Bromine must be performed in a ventilated fume hood. Eliminate ignition sources such as open flames, hot surfaces, steam baths, static electricity, and operation of mechanical and electrical equipment that is not intrinsically safe. Ensure proper grounding and avoid creating static electricity. Be sure to ground metal containers when transferring flammable liquids. Eye protection: Wear safety goggles and face shield when working with liquid bromine. Face protection: Wear a face shield when handling containers of Bromine Gloves: Use or Nitrile Gloves when working with bromine water. Work with liquid bromine requires fluorinated rubber gloves. Clothing: Long closed-toed and closed-heeled shoes, clothing/attire, and Bromine is very hazardous upon skin contact. Special Remarks on Explosion Hazards: REACTS EXPLOSIVELY WITH ACETYLENE, ACRYLONITRILE, AMMONIA, DIMETHYL FORMAMIDE, ETHYL PHOSPHINE, HYDROGEN, ISOBUTYROPHENONE, NICKEL CARBONYL, NITROGEN TRIIODIDE, OZONE, OXYGEN DIFLUORIDE, PHOSPHORUS, POTASSIUM, SILVER AZIDE, SODIUM, & SODIUM CARBIDE. Use in a fume hood and cap container when not in use.
Bromine_Sarpong_Final.docx