Save the experiment and start the measurement by clicking the icon on top of the “Run” software interface. The Software will probably remember you of missing variables like dn/dc but you may still start the measurement. After the measurement is done, set a baseline and stop data recording by clicking the “Stop” icon , otherwise the software will end the measurement after the adjusted measurement time is over. In the tab choose and set the recorded baseline for data evaluation or “Procedures” “baseline” click find baselines”. “auto In the tab choose where you can mark certain data ranges of interest for “Procedures” “Peaks”, further data treatment. We refer to the user-guide for further data treatment (i.e. molar mass evaluation) which can be found in the procedures tab. An overview of the measurement can be found in the tab. “Reports” Data export: Choose Experiment→Graph→Add Custom Plot. Here you can choose how you want the data to be displayed. Do this i.e. for data” and for data in units”. “raw “physical Click File→Export and choose a folder where the file is saved. Choose the export format and the type of data (i.e. raw data or physical units). Normalization Procedure: In to create static light scattering curves the collected data has to be normalized. For different solvents new normalization coefficients have to be evaluated. Prepare a sample of particles with a known particle size and the solvent of interest. The particle size should be about 20 nm or smaller, but you can also use larger particles. However the larger the particle size for normalization, the greater the error of the coefficients. Perform a measurement with this sample as described above. In the tab choose and set a peak for a stable set of data points. “Procedures” “Peaks” Choose Experiment→Configuration→Normalize. Enter the Peak name as well as the radius of the used particles and click The software then calculates the normalization “Normalize”. coefficients for each detector related to the 90° detector signal (detector 11), whose coefficient is set to 1. Remember to rinse the instrument after each experiment and prevent the sample cell from drying out.
Save the experiment and start the measurement by clicking the “Run” icon on top of the software interface. The Software will probably remember you of missing variables like dn/dc but you may still start the measurement. After the measurement is done, set a baseline and stop data recording by clicking the “Stop” icon , otherwise the software will end the measurement after the adjusted measurement time is over. In the “Procedures” tab choose “baseline” and set the recorded baseline for data evaluation or click “auto find baselines”. In the “Procedures” tab choose “Peaks”, where you can mark certain data ranges of interest for further data treatment. We refer to the user-guide for further data treatment (i.e. molar mass evaluation) which can be found in the procedures tab. An overview of the measurement can be found in the “Reports” tab. Data export: Choose Experiment→Graph→Add Custom Plot. Here you can choose how you want the data to be displayed. Do this i.e. for “raw data” and for data in “physical units”. Click File→Export and choose a folder where the file is saved. Choose the export format and the type of data (i.e. raw data or physical units). Normalization Procedure: In to create static light scattering curves the collected data has to be normalized. For different solvents new normalization coefficients have to be evaluated. Prepare a sample of particles with a known particle size and the solvent of interest. The particle size should be about 20 nm or smaller, but you can also use larger particles. However the larger the particle size for normalization, the greater the error of the coefficients. Perform a measurement with this sample as described above. In the “Procedures” tab choose “Peaks” and set a peak for a stable set of data points. Choose Experiment→Configuration→Normalize. Enter the Peak name as well as the radius of the used particles and click “Normalize”. The software then calculates the normalization coefficients for each detector related to the 90° detector signal (detector 11), whose coefficient is set to 1. Remember to rinse the instrument after each experiment and prevent the sample cell from drying out.
wyatt_dawn_heleos_ii_short_instructions.docx