Classical Molecular genetics genetics project project Week Introduction 1 Review of micropipettor use Sterilize control and F2 seeds for analysis of inheritance; spread on plates Begin literature review Week [One day Introduction 2 before lab: Sterilize Col, Put plates Ler, mutant, into growth and F3 seeds; chamber] spread on Continue plates literature review Week Analyze [One day inheritance before lab: Put using F2 plates into seeds growth Plant F2 chamber] seedlings Prepare Col Continue and Ler DNA literature (from genetics review project Plan project: seedlings) discuss with instructor Week Revise Analyze F3 4 project and seedlings prepare Prepare F3 presentation DNA Week Present Set up 1st 5 project to (control) PCR class Begin experiments
Classical genetics project Molecular genetics project Week 1 Introduction Review of micropipettor use Sterilize control and F2 seeds for analysis of inheritance; spread on plates Begin literature review Week 2 [One day before lab: Put plates into growth chamber] Continue literature review Introduction Sterilize Col, Ler, mutant, and F3 seeds; spread on plates Week Analyze inheritance using F2 seeds Plant F2 seedlings Continue literature review Plan project: discuss with instructor [One day before lab: Put plates into growth chamber] Prepare Col and Ler DNA (from genetics project seedlings) Week 4 Revise project and prepare presentation Analyze F3 seedlings Prepare F3 DNA Week 5 Present project to class Begin experiments Set up 1st (control) PCR
Villa-Cuesta%20and%20Hobbie%202016%20Genetic%20Lab_Instructor%20Guidelines_Arabidopsis.docx