Last edited Nov.07 TOXICITY CLINICAL OBSERVATION RECORD Date (day 1) : Investigator: ID: Mouse Strain/sex: Drug: Dose: Route of Admin.! Schedule: Other: Group #: Animal ID: Markings Date: Experiment Day: Nothing Abnormal 0 0 0 0 0 0 0 0 0 0 0 0 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Body Weight (g) 6-10% Loss 1 1 1 1 1 1 1 1 1 1 1 1 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ 11-15% Loss 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ 16-20% Loss 3 3 3 3 3 3 3 3 3 3 3 3 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Hydration Dry Eyes 1 1 1 1 1 1 1 1 1 1 1 1 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Sunken Eyes 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Skin Tenting (1sec) 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Behavioral Observation Abnormal Gait 1 1 1 1 1 1 1 1 1 1 1 1 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Shaking (occasionally) 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Shaking (constantly) 5 5 5 5 5 5 5 5 5 5 5 5 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Signs of Weakness Mild Scruffed fur 1 1 1 1 1 1 1 1 1 1 1 1 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Marked Scruffed fur 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Hunched (while sitting) 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Hunched (sit and walk) 3 3 3 3 3 3 3 3 3 3 3 3 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Paralysis 5 5 5 5 5 5 5 5 5 5 5 5 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Labored Breathing 4 4 4 4 4 4 4 4 4 4 4 4 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Lethargy Slow moving 2 2 2 2 2 2 2 2 2 2 2 2 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Slow when stimulated 3 3 3 3 3 3 3 3 3 3 3 3 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ No response when 5 5 5 5 5 5 5 5 5 5 5 5 ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ stimulated Additional Observations ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Total Score2: 'See additional observations score sheet for # and Letter notation Performed by: 2Any mouse with a score of5 in a single category (other than wt. loss) or overall score of 10 is euthanized. Date: Any mouse with weight loss > 20%is euthanized Additional Observations Score H -head tilt 3 T -tail ulceration or necrosis 2-5 R-skin rash 2-5 ED-edema 3
Last edited Nov.07 TOXICITY CLINICAL OBSERVATION RECORD Date (day 1) : Investigator: ID: Mouse Strain/sex: Drug: Dose: Route of Admin.! Schedule: Other: Group #: Animal ID: Markings Date: Experiment Day: Nothing Abnormal ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 ⁭ 0 Body Weight (g) 6-10% Loss ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 11-15% Loss ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 16-20% Loss ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 Hydration Dry Eyes ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 Sunken Eyes ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 Skin Tenting (1sec) ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 Behavioral Observation Abnormal Gait ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 Shaking (occasionally) ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 Shaking (constantly) ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 Signs of Weakness Mild Scruffed fur ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 ⁭ 1 Marked Scruffed fur ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 Hunched (while sitting) ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 Hunched (sit and walk) ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 Paralysis ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 Labored Breathing ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 ⁭ 4 Lethargy Slow moving ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 ⁭ 2 Slow when stimulated ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 ⁭ 3 No response when stimulated ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 ⁭ 5 Additional Observations ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ ⁭ Total Score2: 'See additional observations score sheet for # and Letter notation 2Any mouse with a score of5 in a single category (other than wt. loss) or overall score of 10 is euthanized. Performed by: Any mouse with weight loss > 20%is euthanized Date: Additional Observations Score H -head tilt 3 T -tail ulceration or necrosis 2-5 R-skin rash 2-5 ED-edema 3
Toxicity%20Monitoring%20%231.docx