b. Select File > Open and select your AD file. c. Turn on the 7900 and select Instrument > Plate Read and press the "Connect". d. If your is not in the 7900, press "Open/Close" and insert the plate. e. Select "Post Read". f. Press "Open/Close" and remove the plate. g. Turn OFF the 7900. 6. Analyze results. If your data have well separated homozygous and heterozygous groupings, then the 7900 can automatically score each sample. If the groups are not well separated, you must manually score the groups. See the Allelic Discrimination getting started guide for more information. a. Select File > Open and select your AD file. b. Select Analysis > Analyze. The data will be scored. c. Select the Results tab. d. If the data are automatically scored, proceed to the next step. If the data are not scored, use the mouse to select data points and from the "Call" box, manually assign the allele calls. e. Select File > Export and save your data report as a tab-delimited file for use with Excel. f. on the plot to save the plot as an image file. g. NOTE: when you save the AD document, the analysis results are NOT SAVED in the AD document. You must export the report.
b. Select File > Open and select your AD file. c. Turn on the 7900 and select Instrument > Plate Read and press the "Connect". d. If your is not in the 7900, press "Open/Close" and insert the plate. e. Select "Post Read". f. Press "Open/Close" and remove the plate. g. Turn OFF the 7900. 6. Analyze results. If your data have well separated homozygous and heterozygous groupings, then the 7900 can automatically score each sample. If the groups are not well separated, you must manually score the groups. See the Allelic Discrimination getting started guide for more information. a. Select File > Open and select your AD file. b. Select Analysis > Analyze. The data will be scored. c. Select the Results tab. d. If the data are automatically scored, proceed to the next step. If the data are not scored, use the mouse to select data points and from the "Call" box, manually assign the allele calls. e. Select File > Export and save your data report as a tab-delimited file for use with Excel. f. on the plot to save the plot as an image file. g. NOTE: when you save the AD document, the analysis results are NOT SAVED in the AD document. You must export the report.
USDA-KSU%20ABI7900%20TaqMan%20Protocol.docx