Write the Dichotomous Key Question 4 for the oxidase test. Your Dichotomous Key Question 4: the full name of George’s organism in proper scientific format. Table 8.5. Expected Results for Gram(-) and Gram(+) Bacteria Gram(-)Bacteria Gram(+) Bacteria Cell Catalase Oxidase Cell Catalase Oxidase Shape Shape Escherichia coli (+) (-) Bacillus subtilis (+) (-) rod rod Haemophilus (+) (+) Staphylococcus (+) (-) haemolyticus rod aureus cocci Proteus vulgaris (+) (-) Bacillus megaterium (+) (+) or (-) rod rod (+) (-) (+) (-) Shigella flexneri Staphylococcus rod saprophyticus cocci Psuedomonas (+) (+) Streptococcus oralis (-) (-) aeruginosa rod cocci Citrobacter freundei (+) (-) Streptococcus (-) (-) rod pneumonia cocci Klebsiella (+) (-) Streptococcus (-) (-) pneumoniae rod pyogenes cocci Serratia marcescens (+) (-) Enterococcus (-) (-) rod faecalis cocci DAY 2: Streak Isolation Results and Interpretation Hopefully, your results show clear isolation like this: (You have different species though and this is a blood plate, not a chocolate agar!) Fig. 8-6. Successful streak isolation of two species on a TSA blood plate. Plate by Leanna Gutierrez, Bio 2192, Spring 2016. On your plate, you should see some big grayish, flat and not shiny colonies. These are Bacillus megaterium. You should also see some tiny colonies. These are Streptococcus pyogenes. Insert a photo of your streak isolation here. Compare it to the picture above.
Write the Dichotomous Key Question 4 for the oxidase test. Your Dichotomous Key Question 4: the full name of George’s organism in proper scientific format. Table 8.5. Expected Results for Gram(-) and Gram(+) Bacteria Gram(-)Bacteria Cell Shape Catalase Oxidase Gram(+) Bacteria Cell Shape Catalase Oxidase Escherichia coli rod (+) (-) Bacillus subtilis rod (+) (-) Haemophilus haemolyticus rod (+) (+) Staphylococcus aureus cocci (+) (-) Proteus vulgaris rod (+) (-) Bacillus megaterium rod (+) (+) or (-) Shigella flexneri rod (+) (-) Staphylococcus saprophyticus cocci (+) (-) Psuedomonas aeruginosa rod (+) (+) Streptococcus oralis cocci (-) (-) Citrobacter freundei rod (+) (-) Streptococcus pneumonia cocci (-) (-) Klebsiella pneumoniae rod (+) (-) Streptococcus pyogenes cocci (-) (-) Serratia marcescens rod (+) (-) Enterococcus faecalis cocci (-) (-) DAY 2: Streak Isolation Results and Interpretation Hopefully, your results show clear isolation like this: (You have different species though and this is a blood plate, not a chocolate agar!) Fig. 8-6. Successful streak isolation of two species on a TSA blood plate. Plate by Leanna Gutierrez, Bio 2192, Spring 2016. On your plate, you should see some big grayish, flat and not shiny colonies. These are Bacillus megaterium. You should also see some tiny colonies. These are Streptococcus pyogenes. Insert a photo of your streak isolation here. Compare it to the picture above.
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