Gavage liver wt ↑ 60d 83.3 5 8.21 ± 1.15 ↔ ↔ ↔ ↑Apopt’s MMP Spleen ↓ K+ lPFOS Water # liver wt ↓ ↑ 0.02% 833.3 50 59.74 ± 12.2 ↓ ↓ ↓ MMP Spleen Tween 80 ↑Apopt’s ↓ In Dong et al. (2012a) a variety of end points have been measured (cell viability, cell cycle, cell apoptosis/necrosis, mitochondrial membrane potential [MMP], gene expression e.g. p53]). Increased apoptosis (decreased cell viability) in spleen and thymus cell suspensions at 5 mg/kg TAD and MMP splenocytes decreased. ≥ Immuno-NOEL splenocyte apoptosis, MMP) = 4.35 mg/L. liver wt is likely adaptive & mediated). (↑ ↓ (↑ PPARα NOEL = 4.35 mg/L based on increased apoptosis and decreased MMP in splenocytes. LOEL = 8.21 mg/L. Dong et 0 0 0.04 ± 0.01 al. (2012b) 8.3 0.5 0.58 ± 0.19 ↔ ↔ ↔ ↔ ↔ ↔ Mouse 16.7 1 4.35 ± 0.63 ↔ ↔ ↔ ↔ ↔ ↔ C57BL/6♂ Gavage IL-1β Macrophages Liver wt ↔ ↔ ↔ ↑ ↑ 83.3 5 8.21 ± 1.15 60d not Others ↔ splenocytes K+ lPFOS 416.7 25 24.53 ± 5.56 IL-β Liver wt ↓ ↓ ↓ ↔ ↑ Water 0.02% Tween 80 IL-1β Cytokines ↑ ↑ 833.3 50 59.74 ± 12.2 Liver wt ex-vivo with in ↓ ↓ ↓ ↑ IL-6 ↑ exposure vivo with & without iv LPS IL-1β ↑ 114.19 ± 2,083 125 Liver wt ↓ ↓ ↓ ↑ 23.72 IL-6 ↑
Gavage 60d lPFOS K+ Water 0.02% Tween 80 83.3 5 8.21 ± 1.15 ↔ ↔ ↔ ↑Apopt’s ↑ liver wt ↓ MMP Spleen 833.3 50 59.74 ± 12.2 ↓ ↓ ↓ ↓ # ↑Apopt’s ↑ liver wt ↓ MMP Spleen In Dong et al. (2012a) a variety of end points have been measured (cell viability, cell cycle, cell apoptosis/necrosis, mitochondrial membrane potential [MMP], gene expression e.g. p53]). Increased apoptosis (decreased cell viability) in spleen and thymus cell suspensions at ≥ 5 mg/kg TAD and MMP splenocytes decreased. Immuno-NOEL (↑ splenocyte apoptosis, ↓ MMP) = 4.35 mg/L. (↑ liver wt is likely adaptive & PPARα mediated). NOEL = 4.35 mg/L based on increased apoptosis and decreased MMP in splenocytes. LOEL = 8.21 mg/L. Dong et al. (2012b) Mouse C57BL/6♂ Gavage 60d lPFOS K+ Water 0.02% Tween 80 0 0 0.04 ± 0.01 8.3 0.5 0.58 ± 0.19 ↔ ↔ ↔ ↔ ↔ ↔ 16.7 1 4.35 ± 0.63 ↔ ↔ ↔ ↔ ↔ ↔ 83.3 5 8.21 ± 1.15 ↔ ↔ ↔ ↑ IL-1β ↔ Others Macrophages not splenocytes ↑ Liver wt 416.7 25 24.53 ± 5.56 ↓ ↓ ↓ ↔ IL-β ↑ Liver wt 833.3 50 59.74 ± 12.2 ↓ ↓ ↓ ↑ IL-1β ↑ IL-6 Cytokines ↑ ex-vivo with in vivo exposure with & without iv LPS ↑ Liver wt 2,083 125 114.19 ± 23.72 ↓ ↓ ↓ ↑ IL-1β ↑ IL-6 ↑ Liver wt
w8.-Immunomodulation-PFASs.docx