Table 12. Summary of QC requirements for water toxicity analysis QC Sample or Minimum Frequency Acceptance Criteria Corrective Action Activity The RPD of four (4) samples utilizing All technician performing Investigate issue. Observe the same matrix must be within Initial Demonstration test must perform once for proper technique. of the results obtained by a of Capability at end of training prior to Retrain as indicated. Repeat IDC’s until trained technician reading an actual sample analysis results within acceptable criteria. independently setup sample Investigate issue. Re-run Method Negative Method One (1) sets of triplicate ≥15 fluorescing organisms for each set Blanks in a known “virgin” testing cell. Control per each batch analysis of three (3) Method Blank cells Repeat until results are within acceptable limits. Investigate issue. Re-run Method Positive Method One (1) sets of triplicate ≥4 non-fluorescing organisms for each Blanks in a known “virgin” testing cell. Control per each batch analysis set of three (3) Method Blank cells Repeat until results are within acceptable limits. Three (3) test chambers filled with six Qualify any results that fail to meet Sample Analysis For each sample analyzed (6) Daphnia magna per each sample criteria due to sample or testing analyzed reagent issues. Investigate issue. If system precision is in control, qualify results. If system One (1) per ten (10) Laboratory Duplicate RPD ≤ 25% precision is out of control, reanalyze samples analyzed entire batch associated with specific QC duplicate. Investigate issue. If system precision is in control, qualify results. If system One (1) per ten (10) Field Duplicate RPD ≤ 25% precision is out of control, reanalyze samples analyzed entire batch associated with specific QC duplicate.
Table 12. Summary of QC requirements for water toxicity analysis QC Sample or Activity Minimum Frequency Acceptance Criteria Corrective Action Initial Demonstration of Capability All technician performing test must perform once at end of training prior to actual sample analysis The RPD of four (4) samples utilizing the same matrix must be within of the results obtained by a trained technician reading an independently setup sample Investigate issue. Observe for proper technique. Retrain as indicated. Repeat IDC’s until results within acceptable criteria. Negative Method Control One (1) sets of triplicate per each batch analysis ≥15 fluorescing organisms for each set of three (3) Method Blank cells Investigate issue. Re-run Method Blanks in a known “virgin” testing cell. Repeat until results are within acceptable limits. Positive Method Control One (1) sets of triplicate per each batch analysis ≥4 non-fluorescing organisms for each set of three (3) Method Blank cells Investigate issue. Re-run Method Blanks in a known “virgin” testing cell. Repeat until results are within acceptable limits. Sample Analysis For each sample analyzed Three (3) test chambers filled with six (6) Daphnia magna per each sample analyzed Qualify any results that fail to meet criteria due to sample or testing reagent issues. Laboratory Duplicate One (1) per ten (10) samples analyzed RPD ≤ 25% Investigate issue. If system precision is in control, qualify results. If system precision is out of control, reanalyze entire batch associated with specific QC duplicate. Field Duplicate One (1) per ten (10) samples analyzed RPD ≤ 25% Investigate issue. If system precision is in control, qualify results. If system precision is out of control, reanalyze entire batch associated with specific QC duplicate.
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