100, 200, 300, 400 and 500 µg at 12th day exposure resulted in 14%, 24%, 42%, 56% and 100% at 15th day with 100, 200, 300, 400 and 500µg exposure it was 26%, 34%, 44%, 62% and 100% respectively. The percentage of mortality was increased with proportionate of the drug concentration in all the stages of embryonic development. The lethal dose (LD50) was calculated directly from the graph drawn between percentage of mortality and concentration of CP in µg. The embryos treated on 3rd day, LD50 was assessed as a dose close to 30 µg while dose of 32µg corresponded to LD50 value of 4th day, while the values 5th , 7th and 12th and 15th day were 44, 79, 172, 320, 376 µg respectively. (Fig. 2.4-2.10). Table. 1.1: Effect of CP on Mortality of chick embryo Day CP Concentration in µg Mortality LD50 (µg) 10 32 20 44 3 day 30 40 56 80 84 100 100 10 30 20 40 4 day 40 52 32 80 76 100 100 10 28 20 38 5 day 44 40 48 80 56 100 100 10 18 20 32 7 day 79 40 46 80 50 100 100 50 16
100, 200, 300, 400 and 500 µg at 12th day exposure resulted in 14%, 24%, 42%, 56% and 100% at 15th day with 100, 200, 300, 400 and 500µg exposure it was 26%, 34%, 44%, 62% and 100% respectively. The percentage of mortality was increased with proportionate of the drug concentration in all the stages of embryonic development. The lethal dose (LD50) was calculated directly from the graph drawn between percentage of mortality and concentration of CP in µg. The embryos treated on 3rd day, LD50 was assessed as a dose close to 30 µg while dose of 32µg corresponded to LD50 value of 4th day, while the values 5th , 7th and 12th and 15th day were 44, 79, 172, 320, 376 µg respectively. (Fig. 2.4-2.10). Table. 1.1: Effect of CP on Mortality of chick embryo Day CP Concentration in µg Mortality LD50 (µg) 3 day 10 32 30 20 44 40 56 80 84 100 100 4 day 10 30 32 20 40 40 52 80 76 100 100 5 day 10 28 44 20 38 40 48 80 56 100 100 7 day 10 18 79 20 32 40 46 80 50 100 100 50 16
UGCMRPFINALREPORTPEAPCODCE.docx