Station 4: Enzymes are Reusable Station 3: Enzymes are Specific Procedure: Place a small piece of avocado into a test tube. Procedure: EIGHT drops of H2O2 onto the avocado. Label two depressions A, B. Record reaction time and bubble rating. Place SIX drops of room temp yeast solution in depression A. Drain ONLY the water from tube! Use the SAME piece of Place SIX drops of Lactase solution in depression B. avocado! Now quickly 3 drops of H2O2 in depression A. Repeat steps 2-5 for 2. Record the reaction time and bubble rating. Repeat steps 2-5 for Trial 3. If the reaction lasts longer than 1 minute, write “>1”. Repeat steps 4-6 for depression B. *NOTE: The liquid that drains off is H2O. The enzyme in the liver broke the H2O2 (the substrate) into H2O and oxygen (the products). Data 4 Data Table 3 Complete Time Bubble Rating Depression for reaction (0-10) Complete Time Bubble Rating Depression for reaction (0-10) A. 1st A. catalase B. 2nd B. lactase C. 3rd Bar Graph 4: Graph the Bubble Rating for each solution Bar Graph 3: Graph the Bubble Rating for each solution 10 10 9 9 8 8 7 7 6 6 5 5 4 4 3 3 2 2 1 1 1st 2nd trial 3rd trial catalase lactase
Station 3: Enzymes are Specific Procedure: Label two depressions A, B. Place SIX drops of room temp yeast solution in depression A. Place SIX drops of Lactase solution in depression B. Now quickly 3 drops of H2O2 in depression A. Record the reaction time and bubble rating. If the reaction lasts longer than 1 minute, write “>1”. Repeat steps 4-6 for depression B. Data Table 3 Depression Complete Time for reaction Bubble Rating (0-10) A. catalase B. lactase Bar Graph 3: Graph the Bubble Rating for each solution 10 9 8 7 6 5 4 3 2 1 catalase lactase Station 4: Enzymes are Reusable Procedure: Place a small piece of avocado into a test tube. EIGHT drops of H2O2 onto the avocado. Record reaction time and bubble rating. Drain ONLY the water from tube! Use the SAME piece of avocado! Repeat steps 2-5 for 2. Repeat steps 2-5 for Trial 3. *NOTE: The liquid that drains off is H2O. The enzyme in the liver broke the H2O2 (the substrate) into H2O and oxygen (the products). Data 4 Depression Complete Time for reaction Bubble Rating (0-10) A. 1st B. 2nd C. 3rd Bar Graph 4: Graph the Bubble Rating for each solution 10 9 8 7 6 5 4 3 2 1 1st 2nd trial 3rd trial
unit_2_packet_spring_2014.docx