Progressive establishment of First pollination Progressive generation of 2400 large pollination and production of generation of 1200 (cumulative) blocks Mchare seeds" Mchare seeds" seeds" 1.4.2 Generation of 200 "At least 2 Mchare chromosome-doubled varieties planted Mchare hybrids as a source of explants for chromosome doubling (see output 11) chromosome doubling initiated" 1.4.3 Generation of Mchare "Field evaluation hybrids with of hybrids " chromosome doubled Embryo rescue of Embryo rescue of Mchare plants: 200 seeds doubled line doubled line crosses and in crosses and in 100 chromosome vitro regeneration vitro regeneration doubled lines NA NA crossed with 200 chromosome 200 chromosome doubled lines improved diploids doubled lines crossed with crossed with improved diploids improved diploids and selfed and selfed " " 1.4.4 2,400 Mchare hybrids Early evaluation evaluation Early evaluation evaluated in Early NA NA ( EET) with trial ( EET) with ( EET) with Evaluation Trials (EET) for 600 hybrids 1200 hybrids 2400 hybrids agronomic performance
establishment of large pollination blocks First pollination and production of Mchare seeds" Progressive generation of 1200 Mchare seeds" Progressive generation of 2400 (cumulative) seeds" 1.4.2 Generation of 200 chromosome-doubled Mchare hybrids "At least 2 Mchare varieties planted as a source of explants for chromosome doubling (see output 11) chromosome doubling initiated" 1.4.3 Generation of Mchare hybrids with chromosome doubled Mchare plants: 200 seeds NA NA 100 chromosome doubled lines crossed with improved diploids " Embryo rescue of doubled line crosses and in vitro regeneration 200 chromosome doubled lines crossed with improved diploids and selfed " "Field evaluation of hybrids Embryo rescue of doubled line crosses and in vitro regeneration 200 chromosome doubled lines crossed with improved diploids and selfed " 1.4.4 2,400 Mchare hybrids evaluated in Early Evaluation Trials (EET) for agronomic performance NA NA Early evaluation ( EET) with 600 hybrids evaluation trial ( EET) with 1200 hybrids Early evaluation ( EET) with 2400 hybrids
WP1.-NARO.EABBP-BMGF-Progress-Technical-report-April-2017.approved.docx