antidiarrheal, antipyretic and analgesic activities. In addition, immunostimulant and inflammatory activities have been observed in chick embryo. Preparation of Andrographis paniculata extract Powdered plant material was soaked in 95% ethanol for 48 h. The extract was distilled and dark green residue was obtained. The remaining ethanol was removed by evaporating the residue under reduced pressure. Toxicity studies of Andrographis paniculata AP extracts at a dose of 1.0, 2.0, 3.0, 4.0, 5.0 mg / egg was given to chick embryo on 12th day. The changes in body weight, liver, heart and brain weight were recorded. Levels of glucose, urea, protein, cholesterol and the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured to ensure the non-toxic nature of the extract. Fixation of optimum dosage for the plant extract AP extract was given different doses 1.0, 2.0, 3.0, 4.0, and 5.0 mg / egg wt / day on 12th day of embryonic development. The dosage which gives maximum protection (as elicited by the activity of ALT and AST) against CP induced hepatic damage was fixed as the optimum dosages. Collection of embryonic tissues After collection of chick embryonic tissues of liver, heart and brain, the tissues were immediately stored in chilled cold saline at -200C. Preparation of embryonic tissue 10% homogenate (pH 7.4) 10% homogenate of the embryonic brain and heart was prepared by grinding it in Tris HCl buffer, pH 7.4. The homogenate was centrifuged at 3000 rpm Remi
antidiarrheal, antipyretic and analgesic activities. In addition, immunostimulant and activities have been observed in chick embryo. Preparation of Andrographis paniculata extract Powdered plant material was soaked in 95% ethanol for 48 h. The extract was distilled and dark green residue was obtained. The remaining ethanol was removed by evaporating the residue under reduced pressure. Toxicity studies of Andrographis paniculata AP extracts at a dose of 1.0, 2.0, 3.0, 4.0, 5.0 mg / egg was given to chick embryo on 12th day. The changes in body weight, liver, heart and brain weight were recorded. Levels of glucose, urea, protein, cholesterol and the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured to ensure the non-toxic nature of the extract. Fixation of optimum dosage for the plant extract AP extract was given different doses 1.0, 2.0, 3.0, 4.0, and 5.0 mg / egg wt / day on 12th day of embryonic development. The dosage which gives maximum protection (as elicited by the activity of ALT and AST) against CP induced hepatic damage was fixed as the optimum dosages. Collection of embryonic tissues After collection of chick embryonic tissues of liver, heart and brain, the tissues were immediately stored in chilled cold saline at -200C. Preparation of embryonic tissue 10% homogenate (pH 7.4) 10% homogenate of the embryonic brain and heart was prepared by grinding it in Tris HCl buffer, pH 7.4. The homogenate was centrifuged at 3000 rpm Remi inflammatory
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