immunocompetent as control animals. Ex-vivo splenocyte responses to antigen stimulation were not altered. In considering the above it is suggested lymphocyte apoptosis in thymus of males (LOEL 20.9 mg/L) may be a more appropriate endpoint for a NOEL in this study, i.e. NOEL of 13.5 mg/L. In females the NOEL was 43.2 mg/L. The authors suggest immune responses in the rat may, in part, be secondary to hepatic changes. Keil et al. mg/kg/d TAD mg/kg 2008 calculated Mouse 0 0.1 1.7 ~ 1 NK at 4 liver wt at 4 ↔ ↔ ↔ ↔ ↔ ↓ ♀ wks old wks but at 8 ↔ Gavage wks ↔ ♂. 1-17 1.0 17 ~9 NK at 4 liver wt at ↔ ↔ ↔ ↔ ↔ ↔ ♂♀ wks old 4 & 8 wks old NK in ↓ ♂, ↔♀, lPFOS at 8 wks old water plus 0.5% Tween 80 5.0 85 ~ 50 B cell CD3+ & NK at 4 Not liver wt ↔ ↔ ↔ ↓ ♀ ↑ ♂ ↔ ↔ CD4+ at wks old. done at ♂♀ 4 wt ↓kid ♀ ↔ ♂ 4 wks old. 4 wks wks old. at 4 wks old. CD3+ ↓ at NK at ↔ ♂♀ ↓ ♂ ♀ CD4+ at 8 ♂ at 8 wks ↔ ♂♀ 8 wks old 8 wks old at 8 ↓ ♂ wks. old. wk old ↔ ♀
immunocompetent as control animals. Ex-vivo splenocyte responses to antigen stimulation were not altered. In considering the above it is suggested lymphocyte apoptosis in thymus of males (LOEL 20.9 mg/L) may be a more appropriate endpoint for a NOEL in this study, i.e. NOEL of 13.5 mg/L. In females the NOEL was 43.2 mg/L. The authors suggest immune responses in the rat may, in part, be secondary to hepatic changes. Keil et al. 2008 Mouse Gavage 1-17 lPFOS in water plus 0.5% Tween 80 mg/kg/d TAD mg/kg calculated 0 0.1 1.7 ~ 1 ↔ ↔ ↔ ↔ NK at 4 wks old ↔ ↓ liver wt ♀ at 4 wks but ↔ at 8 wks ↔ ♂. 1.0 17 ~9 ↔ ↔ ↔ ↔ NK at 4 wks old ↔ ↔ liver wt ♂♀ at 4 & 8 wks old ↓ NK ♂, ↔♀, at 8 wks old 5.0 85 ~ 50 ↔ ↔ ↔ ↓ B cell ♀ ↔ ♂ 4 wks old. ↔ ♂♀ at 8 wks old ↔ CD3+ & CD4+ ♂♀ at 4 wks old. ↓ CD3+ CD4+ ♂ at 8 wks. ↔ NK at 4 wks old. ↓ NK ♂ ♀ at 8 wks old Not done at 4 wks ↓ ♂ at 8 wk old ↔ ♀ ↑ liver wt ♂ ↓kid wt ♀ at 4 wks old. ↔ ♂♀ at 8 wks old.
w8.-Immunomodulation-PFASs.docx