Chapter 5 Ex: The amount of protein in a sample is measured by the samples absorbance of light at a given wavelength. Using standards, a best fit line of absorbance vs. mg protein gave the following parameters: m = 0.01630 sm = 0.00022 b = 0.1040 sb = 0.0026 An unknown sample has an absorbance of 0.246 ± 0.0059. What is the amount of protein in the sample? determine the absolute uncertainty associated with the subtraction: Then convert to relative uncertainty: Determine uncertainty associated with division: Convert back to absolute uncertainty:
Chapter 5 Ex: The amount of protein in a sample is measured by the samples absorbance of light at a given wavelength. Using standards, a best fit line of absorbance vs. mg protein gave the following parameters: m = 0.01630 sm = 0.00022 b = 0.1040 sb = 0.0026 An unknown sample has an absorbance of 0.246 ± 0.0059. What is the amount of protein in the sample? determine the absolute uncertainty associated with the subtraction: Then convert to relative uncertainty: Determine uncertainty associated with division: Convert back to absolute uncertainty:
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