Week [Crosses with Set up PCRs 5 mutants; check plants & crosses] Week [Crosses with Run gels on 6 mutants; check PCRs plants & crosses] Week [Check plants Discuss 7 & crosses; stop results, plan watering plants next if appropriate] experiments; set up PCRs Week [Continue Presentations 8 mapping] of projects [Collect crosses if ready] Week [Collect Discuss 9 crosses if ready; results, harvest mutant identify seed if ready] markers [Continue mapping] Week Sterilize, begin Continue 10 characterization mapping of mutants 1st lab report due Weeks Characterization Continue 11-13 of mutants mapping Week Poster Finish 14 presentation mapping & analyze results Week Lab notebook Final lab 15 due report due Schedule of 2nd example of genetics project lab (starting with existing mutants, parts B+C above)
Week 5 [Crosses with mutants; check plants & crosses] Set up PCRs Week 6 [Crosses with mutants; check plants & crosses] Run gels on PCRs Week 7 [Check plants & crosses; stop watering plants if appropriate] Discuss results, plan next experiments; set up PCRs Week 8 Presentations of projects [Collect crosses if ready] [Continue mapping] Week 9 [Collect crosses if ready; harvest mutant seed if ready] Discuss results, identify markers [Continue mapping] Week 10 Sterilize, begin characterization of mutants Continue mapping 1st lab report due Weeks 11-13 Characterization of mutants Continue mapping Week 14 Poster presentation Finish mapping & analyze results Week 15 Lab notebook due Final lab report due Schedule of 2nd example of genetics project lab (starting with existing mutants, parts B+C above)
Villa-Cuesta%20and%20Hobbie%202016%20Genetic%20Lab_Instructor%20Guidelines_Arabidopsis.docx