switch on the back This option will of the instrument. allow the user to make basic Wait for the absorbance instrument to measurements initialize and at a given complete internal wavelength. From the home screen, press the softkey at the Some plastics bottom right are not labeled “Basic ATC.” and would not be useful for Press the softkey UV labeled nm.” absorbance “Set Enter the desired readings. wavelength using the numeric keypad and press The blank Enter. solution should be identical to Choose cuvettes or as similar as appropriate for the possible to the wavelength. Make solution in sure they are clean which the on the inside and compound to outside and free be measured from scratches or is suspended. stains. This is usually water or an Wearing gloves, fill aqueous one clean cuvette buffer. at least halfway with the solution to be used as a blank. Fill another cuvette at least halfway with the solution to It is best to be tested for handle absorbance. If cuvettes by there is a shortage the optically of cuvettes, the opaque sides blank can be to avoid measured first, then leaving any removed, and the residue on the blank solution clear sides. replaced with the
switch on the back of the instrument. Wait for the instrument to initialize and complete internal From the home screen, press the softkey at the bottom right labeled “Basic ATC.” Press the softkey labeled “Set nm.” Enter the desired wavelength using the numeric keypad and press Enter. Choose cuvettes appropriate for the wavelength. Make sure they are clean on the inside and outside and free from scratches or stains. Wearing gloves, fill one clean cuvette at least halfway with the solution to be used as a blank. Fill another cuvette at least halfway with the solution to be tested for absorbance. If there is a shortage of cuvettes, the blank can be measured first, then removed, and the blank solution replaced with the This option will allow the user to make basic absorbance measurements at a given wavelength. Some plastics are not and would not be useful for UV absorbance readings. The blank solution should be identical to or as similar as possible to the solution in which the compound to be measured is suspended. This is usually water or an aqueous buffer. It is best to handle cuvettes by the optically opaque sides to avoid leaving any residue on the clear sides.
2012_fall_sop_book_9th_ed_1.docx