Figure 4. Adh enzyme activities of cell-free extracts of strains S. cerevisiae AD∆/CaADH1(pABC5)/∆ScADH2 and the controls AD∆ and AD∆/∆ScADH2. Enzyme activities were determined with ethanol as a substrate and the assays were carried out at pH 8.5 with 2 mM NAD and 0.3 mM ethanol. All assays were carried out in triplicate. *P < 0.001.
Figure 4. Adh enzyme activities of cell-free extracts of S. cerevisiae strains AD∆/CaADH1(pABC5)/∆ScADH2 and the controls AD∆ and AD∆/∆ScADH2. Enzyme activities were determined with ethanol as a substrate and the assays were carried out at pH 8.5 with 2 mM NAD and 0.3 mM ethanol. All assays were carried out in triplicate. *P < 0.001.
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