Patient No. of Clinical Histological Diagnosis Prognosis Group Samples Presentation A 3 Normal oral mucosa B 8 Homogeneous or Epithelial keratosis No significant non-homogeneous without dysplasia and malignant leukoplakia without infection potential Candida C 10 Homogeneous or Epithelial keratosis with Potential for non-homogeneous moderate/severe dysplasia malignancy leukoplakia and without high Candida infection D 10 Homogeneous or Chronic hyperplastic Potential for non-homogeneous candidosis (6 had no malignancy leukoplakia” dysplasia and 4 had mild high dysplasia) Table 2. Oligonucleotide primers for RT-PCR. Primer name Sequence (5’ 3’) – rRNARTPCR F GGATTTACTGAAGACTAACTACTG Ca18S rRNA RT and PCR R GAACAACAACCGATCCCTAGT Ca18S F CACTCACGATGGTTCATTCG CaADH1RTPCR primer and PCR R AAGATGGTGCGACATTGG CaADH1RT F CACCCACGATGGTTCTTTCC CaADH2RTPCR primer and PCR R AAGATTGGTGCAACATGGG CaADH2RT 3. Detection of by PAS staining, immunocytochemistry and RT-PCR in four Candida groups of human archival FFPE samples. RT-PCR PASb IMMUNO PASa CaADH1 CaADH2
Patient Group No. of Samples Clinical Presentation Histological Diagnosis Prognosis A 3 Normal oral mucosa B 8 Homogeneous or non-homogeneous leukoplakia Epithelial keratosis without dysplasia and without Candida infection No significant malignant potential C 10 Homogeneous or non-homogeneous leukoplakia Epithelial keratosis with moderate/severe dysplasia and without Candida infection Potential for malignancy high D 10 Homogeneous or non-homogeneous leukoplakia” Chronic hyperplastic candidosis (6 had no dysplasia and 4 had mild dysplasia) Potential for malignancy high Table 2. Oligonucleotide primers for RT-PCR. Primer name Sequence (5’ – 3’) Ca18S rRNARTPCR F GGATTTACTGAAGACTAACTACTG Ca18S rRNA RT and PCR R GAACAACAACCGATCCCTAGT CaADH1RTPCR F CACTCACGATGGTTCATTCG CaADH1RT primer and PCR R AAGATGGTGCGACATTGG CaADH2RTPCR F CACCCACGATGGTTCTTTCC CaADH2RT primer and PCR R AAGATTGGTGCAACATGGG 3. Detection of Candida by PAS staining, immunocytochemistry and RT-PCR in four groups of human archival FFPE samples. PASa PASb IMMUNO RT-PCR CaADH1 CaADH2
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