Metabolic Test Substance Concentration (μg/mL) Exposure Harvest Activation Period Time Absent Test 1 0*, 412, 588, 840*, 1201*, 1715*, 2450* 3 h 24 h Test 2 0*, 36.8, 73.5, 147, 294*, 588*, 840*, 1201, 1715 24 h 24 h Present Test 1 0, 412, 588, 840, 1201*, 1715*, 2450*, 3500 3 h 24 h *Cultures selected for metaphase analysis. Metabolic Activation Test Substance Concentration (µg/mL) Resulting in: Cytotoxicity in Main Precipitation Genotoxic Effect in Test Test Absent Test 1 1611 1715 > 2450 negative ≥ ≥ Test 2 641 840 > 1715 negative ≥ ≥ Present Test 1 2016 1715 > 3500 negative ≥ ≥ Remarks - Results The positive and vehicle controls gave satisfactory responses, confirming the validity of the test system. The test substance did not induce any statistically significant increases in the frequency of cells with aberrations. The test substance was not clastogenic to lymphocytes treated in under the conditions of the test. vitro Biosafety (2011)
Metabolic Activation Test Substance Concentration (μg/mL) Exposure Period Harvest Time Absent Test 1 0*, 412, 588, 840*, 1201*, 1715*, 2450* 3 h 24 h Test 2 0*, 36.8, 73.5, 147, 294*, 588*, 840*, 1201, 1715 24 h 24 h Present Test 1 0, 412, 588, 840, 1201*, 1715*, 2450*, 3500 3 h 24 h *Cultures selected for metaphase analysis. Metabolic Activation Test Substance Concentration (µg/mL) Resulting in: in Test Cytotoxicity in Main Test Precipitation Genotoxic Effect Absent Test 1 ≥ 1611 ≥ 1715 > 2450 negative Test 2 ≥ 641 ≥ 840 > 1715 negative Present Test 1 ≥ 2016 ≥ 1715 > 3500 negative Remarks - Results The positive and vehicle controls gave satisfactory responses, confirming the validity of the test system. The test substance did not induce any statistically significant increases in the frequency of cells with aberrations. The test substance was not clastogenic to lymphocytes treated in vitro under the conditions of the test. Biosafety (2011)
STD1490-FR-FINAL.docx