Table S2. UHPLC MS/MS conditions for the analysis. Where: MW - Molecular weight, tr Retention time. – MW Moleculat Ion Analyte Fragment Ions tr [g/mol] [M+H]+ 102.1 BHL 171.09 172.1 1.83 71.1 154.0 102.2 74.1 HHL 199.12 200.0 3.67 99.2 71.2 102.2 OHL 227.15 228.0 5.46 74.1 109.1 102.2 OdDHL 297.19 298.0 6.91 98.2 95.1 Figure S1. Normalized bioluminescence of AHL bioluminescence reporters E. coli incubated with plasma exposed AHLs: BHL, HHL, OHL, OdDHL grown over 14 hours. AHL were exposed to a helium/oxygen 0.5% plasma plume for 0, 30 seconds, 60 seconds, 120 seconds, 240 seconds. (a) pSB 536 incubated with BHL at 30 E. coli oC, (b) pSB 401 incubated with HHL at 37 oC, (c) pSB 401 incubated E. coli E. coli with OHL at 37 oC and (d) pSB 1142 incubated with OdDHL at 37 oC. Each E. coli data point represents the mean of five replicates with error bars representing the standard deviation.
Table S2. UHPLC MS/MS conditions for the analysis. Where: MW - Molecular weight, tr – Retention time. Analyte MW [g/mol] Moleculat Ion [M+H]+ Fragment Ions tr BHL 171.09 172.1 102.1 1.83 71.1 154.0 HHL 199.12 200.0 102.2 3.67 74.1 99.2 71.2 OHL 227.15 228.0 102.2 5.46 74.1 109.1 OdDHL 297.19 298.0 102.2 6.91 98.2 95.1 Figure S1. Normalized bioluminescence of E. coli AHL bioluminescence reporters incubated with plasma exposed AHLs: BHL, HHL, OHL, OdDHL grown over 14 hours. AHL were exposed to a helium/oxygen 0.5% plasma plume for 0, 30 seconds, 60 seconds, 120 seconds, 240 seconds. (a) E. coli pSB 536 incubated with BHL at 30 oC, (b) E. coli pSB 401 incubated with HHL at 37 oC, (c) E. coli pSB 401 incubated with OHL at 37 oC and (d) E. coli pSB 1142 incubated with OdDHL at 37 oC. Each data point represents the mean of five replicates with error bars representing the standard deviation.
srep26320-s1.docx