Appendix B: Toxicological Investigations Acute toxicity oral – Lumogen Black FK 4281 (50% notified chemical) in 0.5% CMC-solution OECD TG 423 Acute Oral Toxicity Acute Toxic Class Method. – Species/Strain Rat/Wistar Vehicle Doubly distilled water Remarks - Method No significant protocol deviations Number and Sex Dose Mortality of Animals mg/kg bw 3F 5000 0 3F 5000 0 LD50 >5000 mg/kg bw of Toxicity Impaired general state, dyspnoea, staggering and piloerection were observed from 3 to 5 hours after administration. Effects in Organs None observed. Remarks - Results The observed effects may reflect the large dose volume and are not necessarily indicative of absorption of the notified chemical. The notified chemical is of low toxicity via the oral BASF AG (2005d) Acute toxicity inhalation – Lumogen Black FK 4281 (50% notified chemical) OECD TG 403 Acute Inhalation Toxicity Limit Test. – Species/Strain Rat/Wistar Vehicle 1% Aerosil 200 Method of Exposure Oro-nasal exposure. Exposure Period 4 Physical Form aerosol (particulate). Particle Size 1.8 and 2.1 m (two independent samples) Remarks - Method No significant protocol deviations Number and Sex Concentration Mortality of Animals mg/L Nominal* Actual 5 per sex 24.7 5.1 1/10 *The nominal concentration was calculated from the amount of the notified chemical dosed into the dust generator and the supply air flow. LC50 >5.1 mg/L/4 hours Signs of Toxicity Mortality was observed in one female rat 3 hours after exposure. Common abnormalities noted during the study included increased respiratory rate, pulmonary respiratory sounds, hunched posture, smeared fur and black coloured faeces. Fur staining persisted until and including study day 14. All animals attempted to escape at the very beginning of exposure.
Appendix B: Toxicological Investigations Acute toxicity – oral Lumogen Black FK 4281 (50% notified chemical) in 0.5% CMC-solution OECD TG 423 Acute Oral Toxicity – Acute Toxic Class Method. Species/Strain Rat/Wistar Vehicle Doubly distilled water Remarks - Method No significant protocol deviations Number and Sex of Animals Dose mg/kg bw Mortality 3F 5000 0 3F 5000 0 LD50 >5000 mg/kg bw of Toxicity Impaired general state, dyspnoea, staggering and piloerection were observed from 3 to 5 hours after administration. Effects in Organs None observed. Remarks - Results The observed effects may reflect the large dose volume and are not necessarily indicative of absorption of the notified chemical. The notified chemical is of low toxicity via the oral BASF AG (2005d) Acute toxicity – inhalation Lumogen Black FK 4281 (50% notified chemical) OECD TG 403 Acute Inhalation Toxicity – Limit Test. Species/Strain Rat/Wistar Vehicle 1% Aerosil 200 Method of Exposure Oro-nasal exposure. Exposure Period 4 Physical Form aerosol (particulate). Particle Size 1.8 and 2.1 m (two independent samples) Remarks - Method No significant protocol deviations Number and Sex of Animals Concentration mg/L Mortality Nominal* Actual 5 per sex 24.7 5.1 1/10 *The nominal concentration was calculated from the amount of the notified chemical dosed into the dust generator and the supply air flow. LC50 >5.1 mg/L/4 hours Signs of Toxicity Mortality was observed in one female rat 3 hours after exposure. Common abnormalities noted during the study included increased respiratory rate, pulmonary respiratory sounds, hunched posture, smeared fur and black coloured faeces. Fur staining persisted until and including study day 14. All animals attempted to escape at the very beginning of exposure.
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