None. Sarcomas Testing Sample type required Turnaround times: Fluorescence in situ hybridisation (FISH) (test performed on cultured fixed cells /slides) *FFPE tissue section slides (2-4uM) Diagnostic 14 days – Fresh/frozen tissue touch imprint Follow-up (BM Rhabdomyosarcoma FOXO1 – slides infiltration) 21 Ewings (PNET) EWSR1 – Bone marrow (Lithium Heparin– 5ml) days Synovial Sarcoma SS18 (SYT) Infantile Fibrosarcoma/ Congenital Mesoblastic ETV6 Nephroma Dermatofibrosarcoma protuberans (DFSP) Translocation detection by reverse transcription PCR (RT-PCR) (test performed on cDNA) *FFPE rolled sections (5×10um) Diagnostic 28 days – Fresh/frozen tissue Follow-up 28 days Ewings Sarcoma Desmoplastic round cell tumour – EWS-FLI1 EWS-WT1 EWS-ERG Clear cell sarcoma Rhabdomyosarcoma EWS-ATF1 PAX3-FOXO1 Infantile Fibrosarcoma/Congenital Mesoblastic PAX7- FOXO1 Nephroma Synovial Sarcoma ETV6-NTRK3 SS18-SSX1 SS18-SSX2 Current trials participation None. * FFPE: Formalin Fixed Paraffin Embedded tissue
None. Sarcomas Testing Sample type required Turnaround times: Fluorescence in situ hybridisation (FISH) (test performed on cultured fixed cells /slides) *FFPE tissue section slides (2-4uM) Fresh/frozen tissue – touch imprint slides Bone marrow (Lithium Heparin– 5ml) Diagnostic – 14 days Follow-up (BM infiltration) – 21 days Rhabdomyosarcoma FOXO1 Ewings (PNET) EWSR1 Synovial Sarcoma SS18 (SYT) Infantile Fibrosarcoma/ Congenital Mesoblastic Nephroma ETV6 Dermatofibrosarcoma protuberans (DFSP) Translocation detection by reverse transcription PCR (RT-PCR) (test performed on cDNA) *FFPE rolled sections (5×10um) Fresh/frozen tissue Diagnostic – 28 days Follow-up – 28 days Ewings Sarcoma EWS-FLI1 EWS-ERG Rhabdomyosarcoma PAX3-FOXO1 PAX7- FOXO1 Synovial Sarcoma SS18-SSX1 SS18-SSX2 Desmoplastic round cell tumour EWS-WT1 Clear cell sarcoma EWS-ATF1 Infantile Fibrosarcoma/Congenital Mesoblastic Nephroma ETV6-NTRK3 Current trials participation None. * FFPE: Formalin Fixed Paraffin Embedded tissue
Test%20directory%20SIHMDS-AG%20June%202018.pdf.docx