OECD TG 301 B, Ready Biodegradability: CO2 Evolution Test (Modified Sturm Test). Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring Remarks - Method Reference substance sodium benzoate – Toxicity test test substance + reference substance – sodium benzoate Test substance Day % degradation Day % degradation 0 0 0 0 5 5 5 22 10 10 10 54 12 10 12 62 28 13 28 - Remarks - Results Percentage degradation of the reference substance was greater than 60% by day 12, thus validating the study. In the toxicity test the degradation was greater than 25% within 10 days, thus indicating that the test polymer was not inhibitory to the microorganisms in the inoculum. The degradation of the notified polymer did not reach 60% within the 28 days, thus the notified polymer is not readily biodegradable. CCER 2001 8.2. Ecotoxicological investigations 8.2.1. Acute toxicity to fish A very brief report was submitted as part of the Canadian assessment document. DOC750 (notified polymer) OECD TG 203 Fish, Acute Toxicity Test unaerated, static. – Species Oncorhynchus mykiss Exposure Period 96 hours Auxiliary Solvent None Water Hardness Not reported Analytical Monitoring None Remarks Method Temperature was maintained at 12.6-12.8oC. Dissolved oxygen and pH – were measured at the start and end of study and ranged between 10.2 (0 h) and 7.7 8.8 (96 h) mg/L and 7.0-7.1 (0 and 96 h) respectively. – Concentration mg/L Number of Fish Mortality Nominal Actual 1 h 24 h 48 h 72 h 96 h 0 - 10 - 0 0 0 0 1 - 10 - 0 0 0 0 10 - 10 - 0 0 0 0 100 - 10 - 0 0 0 0 1000 - 10 - 4 6 9 10 LC50 316 mg/L (95% CI = 100 1000) at 96 hours. –
OECD TG 301 B, Ready Biodegradability: CO2 Evolution Test (Modified Sturm Test). Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring Remarks - Method Reference substance – sodium benzoate Toxicity test – test substance + reference substance Test substance sodium benzoate Day % degradation Day % degradation 0 0 0 0 5 5 5 22 10 10 10 54 12 10 12 62 28 13 28 - Remarks - Results Percentage degradation of the reference substance was greater than 60% by day 12, thus validating the study. In the toxicity test the degradation was greater than 25% within 10 days, thus indicating that the test polymer was not inhibitory to the microorganisms in the inoculum. The degradation of the notified polymer did not reach 60% within the 28 days, thus the notified polymer is not readily biodegradable. CCER 2001 8.2. Ecotoxicological investigations 8.2.1. Acute toxicity to fish A very brief report was submitted as part of the Canadian assessment document. DOC750 (notified polymer) OECD TG 203 Fish, Acute Toxicity Test – unaerated, static. Species Oncorhynchus mykiss Exposure Period 96 hours Auxiliary Solvent None Water Hardness Not reported Analytical Monitoring None Remarks – Method Temperature was maintained at 12.6-12.8oC. Dissolved oxygen and pH were measured at the start and end of study and ranged between 10.2 (0 h) and 7.7 – 8.8 (96 h) mg/L and 7.0-7.1 (0 and 96 h) respectively. Concentration mg/L Number of Fish Mortality Nominal Actual 1 h 24 h 48 h 72 h 96 h 0 - 10 - 0 0 0 0 1 - 10 - 0 0 0 0 10 - 10 - 0 0 0 0 100 - 10 - 0 0 0 0 1000 - 10 - 4 6 9 10 LC50 316 mg/L (95% CI = 100 – 1000) at 96 hours.
STD1077FR.docx