Time-weighted mean Nominal Concentration Number Mortality (%) measured concentration at 0 hour (mg/L) of Fish (mg/L) 3 h 6 h 24 h 48 h 72 h 96 h Control 0 20 0 0 0 0 0 0 3.2 1.9 20 0 0 0 0 0 0 5.6 3.2 20 0 0 0 0 0 0 10 6.5 20 0 0 0 0 10 25 18 16.0 20 0 5 55 100 100 100 32 32.0 20 0 85 100 100 100 100 LC50 8.8 (7.5 10.0) mg/L at 96 hours (time-weighted mean measured – concentrations) NOEC 3.2 mg/L at 96 (time-weighted mean measured concentrations) Remarks Results All validity criteria for the test were satisfied. All the exposure solutions – containing the test substance were observed to be homogeneous dispersions without a precipitate. Due to a significant decline in measured test concentrations at 72 and 96 it was appropriate to use the time-weighted mean measured concentrations for the analyses. Analysis of the mortality (LC50) was done by the moving average method (Thompson, 1947) based on the time-weighted mean measured test concentrations. The notified chemical is toxic to fish Safepharm (1998f) Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 sp. Acute Immobilisation Test Static Test Daphnia – Species Daphnia Exposure Period 48 hours Auxiliary Solvent Not reported Water Hardness Not reported Analytical Monitoring Spectrophotometer (Perkin-Elmer Lambda 20 or equivalent) Remarks - Method The test was conducted according to the guidelines above and good laboratory practice (GLP) principles. No significant deviations from the test guidelines were reported. Nominal Time-weighted mean Number of Cumulative % Immobilised Concentration measured concentration D. magna (mg/L) (mg/L) 24 h 48 h Control 0 20 0 0 1.0 0.5 20 0 0 1.8 1.3 20 0 0 3.2 2.4 20 0 0 5.6 3.4 20 0 0 10.0 7.8 20 0 0 18.0 13.0 20 0 0 32.0 24.0 20 0 20 56.0 33.0 20 0 75 100.0 76.0 20 0 100 EC50 23.0 (20.0 27.0) mg/L at 48 hours (time-weighted mean measured – concentrations of filtered media)
Nominal Concentration at 0 hour (mg/L) Time-weighted mean measured concentration (mg/L) Number of Fish Mortality (%) 3 h 6 h 24 h 48 h 72 h 96 h Control 0 20 0 0 0 0 0 0 3.2 1.9 20 0 0 0 0 0 0 5.6 3.2 20 0 0 0 0 0 0 10 6.5 20 0 0 0 0 10 25 18 16.0 20 0 5 55 100 100 100 32 32.0 20 0 85 100 100 100 100 LC50 8.8 (7.5 – 10.0) mg/L at 96 hours (time-weighted mean measured concentrations) NOEC 3.2 mg/L at 96 (time-weighted mean measured concentrations) Remarks – Results All validity criteria for the test were satisfied. All the exposure solutions containing the test substance were observed to be homogeneous dispersions without a precipitate. Due to a significant decline in measured test concentrations at 72 and 96 it was appropriate to use the time-weighted mean measured concentrations for the analyses. Analysis of the mortality (LC50) was done by the moving average method (Thompson, 1947) based on the time-weighted mean measured test concentrations. The notified chemical is toxic to fish Safepharm (1998f) Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test – Static Test Species Daphnia Exposure Period 48 hours Auxiliary Solvent Not reported Water Hardness Not reported Analytical Monitoring Spectrophotometer (Perkin-Elmer Lambda 20 or equivalent) Remarks - Method The test was conducted according to the guidelines above and good laboratory practice (GLP) principles. No significant deviations from the test guidelines were reported. Nominal Concentration Time-weighted mean measured concentration Number of D. magna Cumulative % Immobilised (mg/L) (mg/L) 24 h 48 h Control 0 20 0 0 1.0 0.5 20 0 0 1.8 1.3 20 0 0 3.2 2.4 20 0 0 5.6 3.4 20 0 0 10.0 7.8 20 0 0 18.0 13.0 20 0 0 32.0 24.0 20 0 20 56.0 33.0 20 0 75 100.0 76.0 20 0 100 EC50 23.0 (20.0 – 27.0) mg/L at 48 hours (time-weighted mean measured concentrations of filtered media)
STD1498-FR-FINAL.DOCX