Appendix C: Environmental Fate and Ecotoxicological Investigations Environmental Fate biodegradability Notified chemicals OECD TG 301 F Biodegradability: Manometric Respirometry Test. Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring The biochemical oxygen demand (BOD) was measured; The dissolved organic carbon (DOC) was measured at the beginning and the end of the test. Remarks - Method Test was conducted in triplicates at 22 ± 1˚C for up to 28 days. Aniline was used as the reference substance for reference control test. Test substance Aniline Day % Degradation Day % Degradation 7 0 7 58 28 0 28 80 Remarks - Results All criteria for test validity were met. The reference control test for aniline reached a biodegradation degree of 69% after 14 days. The notified chemicals are not considered readily biodegradable. BASF (2006c) Bioaccumulation Remarks Although the theoretical log KOW value of 7.52 for the notified chemicals indicates high potential for bioaccumulation, this is more a reflection of its very low water solubility but relatively higher (but still low) solubility in n-octanol. The low n-octanol solubility indicates low potential for the notified chemical to dissolve and accumulate significantly in fish tissue. The notifier supports this argument with reference to the Critical Body Burden concept, which establishes that the octanol solubility is well below a critical concentration (0.002×Molecular weight (g/mol)) below which a reduced uptake of the substance can be expected and toxicity is not likely. As a further supporting argument, a low bioconcentration factor (BCF) of 1.6 is calculated from the BCF model Oasis CATABOL model. Ecotoxicological Investigations Acute toxicity to fish Notified chemicals OECD TG 203 Fish, Acute Toxicity Test Static. – EC Directive 92/69/EEC C.1 Toxicity for Fish Static. – Species Danio rerio Exposure Period 96 hours
Appendix C: Environmental Fate and Ecotoxicological Investigations Environmental Fate biodegradability Notified chemicals OECD TG 301 F Biodegradability: Manometric Respirometry Test. Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring The biochemical oxygen demand (BOD) was measured; The dissolved organic carbon (DOC) was measured at the beginning and the end of the test. Remarks - Method Test was conducted in triplicates at 22 ± 1˚C for up to 28 days. Aniline was used as the reference substance for reference control test. Test substance Aniline Day % Degradation Day % Degradation 7 0 7 58 28 0 28 80 Remarks - Results All criteria for test validity were met. The reference control test for aniline reached a biodegradation degree of 69% after 14 days. The notified chemicals are not considered readily biodegradable. BASF (2006c) Bioaccumulation Remarks Although the theoretical log KOW value of 7.52 for the notified chemicals indicates high potential for bioaccumulation, this is more a reflection of its very low water solubility but relatively higher (but still low) solubility in n-octanol. The low n-octanol solubility indicates low potential for the notified chemical to dissolve and accumulate significantly in fish tissue. The notifier supports this argument with reference to the Critical Body Burden concept, which establishes that the octanol solubility is well below a critical concentration (0.002×Molecular weight (g/mol)) below which a reduced uptake of the substance can be expected and toxicity is not likely. As a further supporting argument, a low bioconcentration factor (BCF) of 1.6 is calculated from the BCF model Oasis CATABOL model. Ecotoxicological Investigations Acute toxicity to fish Notified chemicals OECD TG 203 Fish, Acute Toxicity Test – Static. EC Directive 92/69/EEC C.1 Toxicity for Fish – Static. Species Danio rerio Exposure Period 96 hours
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