Open the sample compartment. Insert the blank cuvette in the sample holder labeled Orient “B”. the cuvette such that the optically clear sides are aligned with the light path. Insert the sample cuvette in the first sample holder, labeled If there “1”. are additional samples and enough cuvettes to hold them, insert these in the other four sample holders and note their positions. Close the sample compartment. A 1/10 dilution Locate the is a good numbered sample place to start. selection keys Use the blank (arranged in a solution to circle on the dilute the keypad). “B” to sample. If the select the blank. absorbance is still too high, the softkey dilute again labeled and repeat “Measure blank”. The display until an should read 0.000A. accurate measurement Press the sample is obtained. selection key with Then, multiply the number of the the sample you wish to absorbance analyze. The reading by the sample holder dilution factor carousel will rotate to obtain the and the instrument absorbance will analyze the of the original selected sample, concentration.
Open the sample compartment. Insert the blank cuvette in the sample holder labeled “B”. Orient the cuvette such that the optically clear sides are aligned with the light path. Insert the sample cuvette in the first sample holder, labeled “1”. If there are additional samples and enough cuvettes to hold them, insert these in the other four sample holders and note their positions. Close the sample compartment. Locate the numbered sample selection keys (arranged in a circle on the keypad). “B” to select the blank. the softkey labeled “Measure blank”. The display should read 0.000A. Press the sample selection key with the number of the sample you wish to analyze. The sample holder carousel will rotate and the instrument will analyze the selected sample, A 1/10 dilution is a good place to start. Use the blank solution to dilute the sample. If the absorbance is still too high, dilute again and repeat until an accurate measurement is obtained. Then, multiply the absorbance reading by the dilution factor to obtain the absorbance of the original concentration.
2012_fall_sop_book_9th_ed_1.docx