Figure 3. Adh enzyme activities of Ni-NTA-purified cell-free extracts of recombinant S. strains AD/CaADH1-his, AD/CaADH2-his and the control strain AD/pABC3-his. cerevisiae Enzyme activities were determined with ethanol as a substrate and the assays were carried out at pH 8.5 with 2 mM NAD and 0.3 mM ethanol. All assays were carried out in triplicate. *P < 0.01.
Figure 3. Adh enzyme activities of Ni-NTA-purified cell-free extracts of recombinant S. cerevisiae strains AD/CaADH1-his, AD/CaADH2-his and the control strain AD/pABC3-his. Enzyme activities were determined with ethanol as a substrate and the assays were carried out at pH 8.5 with 2 mM NAD and 0.3 mM ethanol. All assays were carried out in triplicate. *P < 0.01.
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