Following the preliminary range-finding test, green algae was exposed to an aqueous solution of the test substance at a nominal concentration of 0.009 mg/L (six replicates) under constant illumination and shaking at 24 ± 1 °C. Samples of the algal population were removed daily and cell concentrations determined for each control and treated group. Due to the limited solubility of the test substance in the test medium, the highest attainable test concentration was determined to be 0.011 mg/L based on geometric mean measured concentrations. The notified chemical is not harmful to algae up to the limit of its solubility. Harlan Inhibition of microbial activity Notified chemical OECD TG 209 Activated Sludge, Respiration Inhibition Test. Inoculum Activated sludge Exposure Period 3 hours Concentration Range Nominal: Control, 10, 32, 100, 320 and 1,000 mg/L Actual: Not determined Remarks Method Amounts of test substance (5, 16, 50, 160 and 500 mg) were each – separately dispersed into 250 mL of water, followed with ultrasonication for 15 minutes and stirring for 24 hours. Synthetic sewage, activated sludge and water were added to a final volume to give the nominal concentrations of 10, 32, 100, 320 and 1,000 mg/L. The test was conducted in accordance with the test guidelines above and in compliance with GLP standards and principles. IC50 1,000 mg/L at 3 hours NOEC 1,000 mg/L at 3 hours Remarks Results In some instances, initial and final dissolved oxygen concentrations were – below those recommended in the test guideline. This was considered to have no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace. Variation in respiration of controls 1 and 2 was ± 2%, within 15% range. The EC50 (3 hour contact) for 3,5-dichlorophenol, the reference test material, was determined to be 8.3 mg/L which is within the range of mg/L. Therefore, the results for the study are considered valid. The notified chemical is not expected to inhibit microbial respiration in the test sludge. Harlan (2010b)
Following the preliminary range-finding test, green algae was exposed to an aqueous solution of the test substance at a nominal concentration of 0.009 mg/L (six replicates) under constant illumination and shaking at 24 ± 1 °C. Samples of the algal population were removed daily and cell concentrations determined for each control and treated group. Due to the limited solubility of the test substance in the test medium, the highest attainable test concentration was determined to be 0.011 mg/L based on geometric mean measured concentrations. The notified chemical is not harmful to algae up to the limit of its solubility. Harlan Inhibition of microbial activity Notified chemical OECD TG 209 Activated Sludge, Respiration Inhibition Test. Inoculum Activated sludge Exposure Period 3 hours Concentration Range Nominal: Control, 10, 32, 100, 320 and 1,000 mg/L Actual: Not determined Remarks – Method Amounts of test substance (5, 16, 50, 160 and 500 mg) were each separately dispersed into 250 mL of water, followed with ultrasonication for 15 minutes and stirring for 24 hours. Synthetic sewage, activated sludge and water were added to a final volume to give the nominal concentrations of 10, 32, 100, 320 and 1,000 mg/L. The test was conducted in accordance with the test guidelines above and in compliance with GLP standards and principles. IC50 1,000 mg/L at 3 hours NOEC 1,000 mg/L at 3 hours Remarks – Results In some instances, initial and final dissolved oxygen concentrations were below those recommended in the test guideline. This was considered to have no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace. Variation in respiration of controls 1 and 2 was ± 2%, within 15% range. The EC50 (3 hour contact) for 3,5-dichlorophenol, the reference test material, was determined to be 8.3 mg/L which is within the range of mg/L. Therefore, the results for the study are considered valid. The notified chemical is not expected to inhibit microbial respiration in the test sludge. Harlan (2010b)
STD-1444-FR_Final.docx