Supplementary Information: Non-thermal Plasma Exposure Rapidly Attenuates Bacterial AHL-Dependent Quorum Sensing and Virulence Padrig B. Flynn1,2,†, Alessandro Busetti1,†, Ewa Wielogorska3, Olivier P. Chevallier3, Christopher T. Elliott3, Garry Laverty1, Sean P. Gorman1, William G. Graham2 and Brendan F. Gilmore1, *. S1. Function, antibiotic resistance and cognitive HSL of strains used in study. Strain Genotype or phenotype Cognitive HSL Source/ references HSL sensor strain; contains C4-C12 3-oxo-HSLs 32 A. tumefaciens ATCC BAA traR and traG-lacZ fusion, a 3-hydroxy- and 2240 reporter, - HSLs β-galactosidase β and CnR HSL sensor strain; miniTn-5 C4-C8 HSLs. 29 C. violaceum CV026 mutant of CV31532, violacein reporter, KmR HSL sensor strain; contains C6-C8-HSL and 33 Escherichia coli JM109 (pSB401) related medium side luxRI::luxCDABE, bioluminescent reporter, chain HSL analogues TcR E. coli JM109 HSL Sensor strain; contains C4-HSLs and related 33 ahyR ahyI::luxCDABE; (pSB 536) side chain HSL AmpR ColE1 origin analogues JM109 HSL sensor strain; contains C10-C14 long side 33 E. coli (pSB 1142) chain HSL analogues lasR and promoter of lasI::luxCDABE, bioluminescent reporter, TcR and mutant of BHL and OdDHL 24 PAO-MW1 lasI rhlI P. HgClR & TcR aeruginosa,
Supplementary Information: Non-thermal Plasma Exposure Rapidly Attenuates Bacterial AHL-Dependent Quorum Sensing and Virulence Padrig B. Flynn1,2,†, Alessandro Busetti1,†, Ewa Wielogorska3, Olivier P. Chevallier3, Christopher T. Elliott3, Garry Laverty1, Sean P. Gorman1, William G. Graham2 and Brendan F. Gilmore1, *. S1. Function, antibiotic resistance and cognitive HSL of strains used in study. Strain Genotype or phenotype Cognitive HSL Source/ references A. tumefaciens ATCC BAA 2240 HSL sensor strain; contains traR and traG-lacZ fusion, a β-galactosidase reporter, β and CnR C4-C12 3-oxo-HSLs 3-hydroxy- and HSLs 32 C. violaceum CV026 HSL sensor strain; miniTn-5 mutant of CV31532, violacein reporter, KmR C4-C8 HSLs. 29 Escherichia coli JM109 (pSB401) HSL sensor strain; contains luxRI::luxCDABE, bioluminescent reporter, TcR C6-C8-HSL and related medium side chain HSL analogues 33 E. coli JM109 (pSB 536) HSL Sensor strain; contains ahyR ahyI::luxCDABE; AmpR ColE1 origin C4-HSLs and related side chain HSL analogues 33 E. coli JM109 (pSB 1142) HSL sensor strain; contains lasR and promoter of lasI::luxCDABE, bioluminescent reporter, TcR C10-C14 long side chain HSL analogues 33 PAO-MW1 lasI and rhlI mutant of P. aeruginosa, HgClR & TcR BHL and OdDHL 24 -
srep26320-s1.docx