The picture on the left shows fluorescence as excited by a 488 nm laser. The green fluorescence is indicative of acriflavinium chloride adsorption. The picture on the right shows the presence Sample 2 of Alexa-647-labled secondary antibodies as E. Coli Antibody excited by a 640 nm laser Sample (brightness enhanced by 50%). The presence of secondary antibodies in Sample 2 but not in Sample 1 shows that the secondary antibodies are present due to the successful conjugation of primary antibodies. The picture on the left shows fluorescence as excited by a 488 nm laser, which is indicative of acriflavinium chloride adsorption. The picture on the right shows the lack of fluorescence excited Sample 3 by a 640 nm laser (brightness enhanced by 50%). The lack Cryptosporidium of fluorescence at 640 nm Control shows that the secondary antibodies are not binding to the sample due to the structure of the hydrogel or the presence of acriflavinium chloride.
Sample 2 E. Coli Antibody Sample The picture on the left shows fluorescence as excited by a 488 nm laser. The green fluorescence is indicative of acriflavinium chloride adsorption. The picture on the right shows the presence of Alexa-647-labled secondary antibodies as excited by a 640 nm laser (brightness enhanced by 50%). The presence of secondary antibodies in Sample 2 but not in Sample 1 shows that the secondary antibodies are present due to the successful conjugation of primary antibodies. Sample 3 Cryptosporidium Control The picture on the left shows fluorescence as excited by a 488 nm laser, which is indicative of acriflavinium chloride adsorption. The picture on the right shows the lack of fluorescence excited by a 640 nm laser (brightness enhanced by 50%). The lack of fluorescence at 640 nm shows that the secondary antibodies are not binding to the sample due to the structure of the hydrogel or the presence of acriflavinium chloride.
2013-FALL-AY-REU_Project7_Microbial_Final-Technical-Paper.docx