OECD TG 201 Alga, Growth Inhibition Test. Species Scenedesmus suspicatus Exposure Period 72 hours Concentration Range 100 mg/L (Filtered) Nominal Water Hardness 24 mg Analytical Monitoring Test solutions were not measured Algae were exposed to the filtrate of a super saturated stock suspension of the notified chemical at a nominal concentration of 100 mg/L under constant illumination and shaking. After 72 h, there was no significant inhibition of algal growth or biomass at the nominal concentration of 100 mg/L. The ecotoxicity data indicates the notified chemical is not toxic to algae up to the limit of its solubility. IBACON GmbH, Germany (1999k) 8.2.4. Inhibition of microbial activity Urea 1 OECD TG 209 Activated Sludge, Respiration Inhibition Test. Inoculum Activated sludge Exposure Period 3 Concentration Range 10, 32, 100, 320, 1000 mg/L Nominal The activated sludge study was conducted using sludge obtained from sewage treatment plant in Groβ-Zimmern, Germany. The definitive study was conducted on nominal concentrations of 10, 32, 100, 320 and 1000 mg/L. Amounts of test material (5, 16, 50, 160 and 500 mg) were added to water (284 mL) and sewerage (16 mL) and samples were stirred continuously. The reference material used in the study was When compared to the control, activated sludge after 3 h experienced differences in respiration of between to 5.7%. The –6.6 EC50 for the notified substance to activated sludge could not be quantified. However, the 3-hour EC50 for the notified substance to activated sludge is expected to be greater than 1000 mg/L. The EC50 of the reference substance was 9 mg/L, therefore confirming the suitability of the activated sludge. EC50 > 1000 mg/L NOEC Not determined The ecotoxicity data indicates the notified chemical is not toxic to activated sludge up to 1000 mg/L suspension. IBACON GmbH, Germany 9. RISK ASSESSMENT 9.1. Environment 9.1.1. Environment exposure assessment – The new chemical will be used as a component of a ready-to-use grease at one mining site in Western Australia. The majority of the import volume will be collected and disposed of by
OECD TG 201 Alga, Growth Inhibition Test. Species Scenedesmus suspicatus Exposure Period 72 hours Concentration Range Nominal 100 mg/L (Filtered) Water Hardness 24 mg Analytical Monitoring Test solutions were not measured Algae were exposed to the filtrate of a super saturated stock suspension of the notified chemical at a nominal concentration of 100 mg/L under constant illumination and shaking. After 72 h, there was no significant inhibition of algal growth or biomass at the nominal concentration of 100 mg/L. The ecotoxicity data indicates the notified chemical is not toxic to algae up to the limit of its solubility. IBACON GmbH, Germany (1999k) 8.2.4. Inhibition of microbial activity Urea 1 OECD TG 209 Activated Sludge, Respiration Inhibition Test. Inoculum Activated sludge Exposure Period 3 Concentration Range Nominal 10, 32, 100, 320, 1000 mg/L The activated sludge study was conducted using sludge obtained from sewage treatment plant in Groβ-Zimmern, Germany. The definitive study was conducted on nominal concentrations of 10, 32, 100, 320 and 1000 mg/L. Amounts of test material (5, 16, 50, 160 and 500 mg) were added to water (284 mL) and sewerage (16 mL) and samples were stirred continuously. The reference material used in the study was When compared to the control, activated sludge after 3 h experienced differences in respiration of between –6.6 to 5.7%. The EC50 for the notified substance to activated sludge could not be quantified. However, the 3-hour EC50 for the notified substance to activated sludge is expected to be greater than 1000 mg/L. The EC50 of the reference substance was 9 mg/L, therefore confirming the suitability of the activated sludge. EC50 > 1000 mg/L NOEC Not determined The ecotoxicity data indicates the notified chemical is not toxic to activated sludge up to 1000 mg/L suspension. IBACON GmbH, Germany 9. RISK ASSESSMENT 9.1. Environment 9.1.1. Environment – exposure assessment The new chemical will be used as a component of a ready-to-use grease at one mining site in Western Australia. The majority of the import volume will be collected and disposed of by
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