Following two range finding tests, a definitive test was conducted at with concentrations 0.464 21.50 mg/L under static conditions for a period of – 96 h. Test conditions were: 20-21°C, pH 7.3 7.7, 8.0 8.7 O2 mg/L, 8 – – hours dark and 16 hours light period. The LC50 was calculated using probit analysis. Mortality Nominal Number of Fish 1 h 4 h 24 h 48 h 72 h 96 h Concentration mg/L Control 10 0 0 0 0 0 0 0.464 10 0 0 0 0 0 0 1.000 10 0 0 0 0 0 0 2.150 10 0 0 0 0 0 0 4.640 10 0 0 0 0 0 0 10.00 10 0 0 10 10 10 10 21.50 10 0 0 10 10 10 10 LC50 4.6 10 mg/L at 96 hours (based on nominal concentrations) – NOEC 4.64 mg/L at 96 hours (based on nominal concentrations) Remarks Results No undissolved test substance was observed and no abnormalities were – observed in live fish. The test substance is toxic to fish BASF (1989) A.2.2. toxicity to aquatic invertebrates The analogue chemical (purity ca. 90%) Guideline for testing of chemicals EG-1 of Jan 1982 (EPA Office of Substances) - Static Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent dimethylsulfoxide Water Hardness 2.6 mmol/L Analytical Monitoring None reported Remarks - Method Only a summary report was provided. The test substance and dimethylsulfoxide (dispersing agent) were added to the dilution water at 50 and 100 mg/L respectively. The stock suspension was stirred for 17 hours at 25°C. Serial dilution was used to prepare the test solutions. A definitive test was conducted with test substance concentration 0.098 – 3.13 mg/L. Test conditions were: 19.8°C, pH 7.9–8.2, 7.5–9.0 mg O2/L, 8 hours dark and 16 hours light period. The EC50 after 48 hours was determined by the Spearman-Karber method. Number Immobilised
Following two range finding tests, a definitive test was conducted at with concentrations 0.464 – 21.50 mg/L under static conditions for a period of 96 h. Test conditions were: 20-21°C, pH 7.3 – 7.7, 8.0 – 8.7 O2 mg/L, 8 hours dark and 16 hours light period. The LC50 was calculated using probit analysis. Mortality Nominal Concentration mg/L Number of Fish 1 h 4 h 24 h 48 h 72 h 96 h Control 10 0 0 0 0 0 0 0.464 10 0 0 0 0 0 0 1.000 10 0 0 0 0 0 0 2.150 10 0 0 0 0 0 0 4.640 10 0 0 0 0 0 0 10.00 10 0 0 10 10 10 10 21.50 10 0 0 10 10 10 10 LC50 4.6 – 10 mg/L at 96 hours (based on nominal concentrations) NOEC 4.64 mg/L at 96 hours (based on nominal concentrations) Remarks – Results No undissolved test substance was observed and no abnormalities were observed in live fish. The test substance is toxic to fish BASF (1989) A.2.2. toxicity to aquatic invertebrates The analogue chemical (purity ca. 90%) Guideline for testing of chemicals EG-1 of Jan 1982 (EPA Office of Substances) - Static Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent dimethylsulfoxide Water Hardness 2.6 mmol/L Analytical Monitoring None reported Remarks - Method Only a summary report was provided. The test substance and dimethylsulfoxide (dispersing agent) were added to the dilution water at 50 and 100 mg/L respectively. The stock suspension was stirred for 17 hours at 25°C. Serial dilution was used to prepare the test solutions. A definitive test was conducted with test substance concentration 0.098 – 3.13 mg/L. Test conditions were: 19.8°C, pH 7.9–8.2, 7.5–9.0 mg O2/L, 8 hours dark and 16 hours light period. The EC50 after 48 hours was determined by the Spearman-Karber method. Number Immobilised
STD1399FR.docx