100 20 0 0 LC50 37.3 mg/L (95%CI: 29.8-46.6) at 48 hours Remarks - Results The test concentrations remained stable with recovery >80% and thus nominal concentrations were presented without transformation. The notified polymer is considered to be harmful to daphnia. BASF Aktiengesellschaft (2001d) 8.2.3. Algal growth inhibition test Analogue polymer [Lutensol XL80 - details are claimed exempt from publication] OECD TG 201 Alga Growth Inhibition Test. EC Directive 92/69/EEC C.3 Algal Inhibition Test. US EPA OPPTS 850.5400 Alga Toxicity Test. Species CHODAT SAG 86.81 Desmodesmus subspicatus Exposure Period 96 hours Concentration Range Nominal 0.391, 0.781, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L Auxiliary Solvent None Medium Deionised water Water Hardness Not reported Analytical Monitoring In vivo chlorophyll-a-fluorescence at 0, 24, 48, 72, and 96 h Remarks - Method Three replicates per each test concentration and five replicates for the control were used. The stability of the test substance was guaranteed by the sponsor and thus no concentration control analysis were performed. The pH and temperature remained within acceptable limits during the test. The EC50 values were determined by linear regression analysis of the concentration response relationship. Biomass Growth Nominal EbC50 Nominal Nominal ErC50 Nominal mg/L at 96 h mg/L at 72 h mg/L at 96 h mg/L at 72 h 23.2 6.25 40.2 6.25 Remarks - Results The test appears to meet the validity criteria. The analogue polymer is considered to be harmful to alga. BASF Aktiengesellschaft (2003b) 8.2.4. Inhibition of microbial activity Remarks Test was not conducted. 9. RISK ASSESSMENT 9.1. Environment 9.1.1. Environment exposure assessment – Potentially all of the notified polymer for industrial uses will be released into the when used cleaning water is released. The notified polymer is considered to be relatively soluble in water
100 20 0 0 LC50 37.3 mg/L (95%CI: 29.8-46.6) at 48 hours Remarks - Results The test concentrations remained stable with recovery >80% and thus nominal concentrations were presented without transformation. The notified polymer is considered to be harmful to daphnia. BASF Aktiengesellschaft (2001d) 8.2.3. Algal growth inhibition test Analogue polymer [Lutensol XL80 - details are claimed exempt from publication] OECD TG 201 Alga Growth Inhibition Test. EC Directive 92/69/EEC C.3 Algal Inhibition Test. US EPA OPPTS 850.5400 Alga Toxicity Test. Species Desmodesmus subspicatus CHODAT SAG 86.81 Exposure Period 96 hours Concentration Range Nominal 0.391, 0.781, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L Auxiliary Solvent None Medium Deionised water Water Hardness Not reported Analytical Monitoring In vivo chlorophyll-a-fluorescence at 0, 24, 48, 72, and 96 h Remarks - Method Three replicates per each test concentration and five replicates for the control were used. The stability of the test substance was guaranteed by the sponsor and thus no concentration control analysis were performed. The pH and temperature remained within acceptable limits during the test. The EC50 values were determined by linear regression analysis of the concentration response relationship. Biomass Growth Nominal EbC50 Nominal Nominal ErC50 Nominal mg/L at 96 h mg/L at 72 h mg/L at 96 h mg/L at 72 h 23.2 6.25 40.2 6.25 Remarks - Results The test appears to meet the validity criteria. The analogue polymer is considered to be harmful to alga. BASF Aktiengesellschaft (2003b) 8.2.4. Inhibition of microbial activity Remarks Test was not conducted. 9. RISK ASSESSMENT 9.1. Environment 9.1.1. Environment – exposure assessment Potentially all of the notified polymer for industrial uses will be released into the when used cleaning water is released. The notified polymer is considered to be relatively soluble in water
STD1157FR.docx