4 NE 0 NE 5 NE 0 NE 6 NE 0 0 Conjunctiva r c d r c d r C d 1 1 0 NE 0 0 0 0 0 0 2 1 0 NE 1 0 0 0 0 0 3 1 1 NE 1 0 0 0 0 0 4 0 0 NE 0 0 0 NE NE 5 1 0 NE 0 0 0 NE NE 6 1 0 NE 1 0 0 0 0 0 1 see Attachment 1 for Draize scales O = opacity, a = area, r = redness, c = chemosis, d = discharge, NE = Not estimated The notified chemical was slightly irritating to the eyes of rabbits. Result: Eye irritation with free acid (Younger Laboratories, 1968b) New Zealand White rabbit Species/strain: 3 (2 males, 1 female) Number/sex of animals: 1 to 7 days after treatment Observation period: 100 mg of finely ground free acid powder were placed into the Method of administration: conjunctival sac of the right eye of each rabbit. The eyes were examined for signs of irritation after 1, 24, 48, 72, 120 and 168 hours. The treated eyes were rinsed with warm isotonic saline 24 hours after treatment later. After the reading, fluorescein (concentration not stated) was instilled to detect corneal damage. Not stated Test method: Treated eyes were graded according to the severity of erythema, oedema, discharge, corneal Comment: opacity and iridial reactions on an arbitrary scale of 0 to 110. Moderate discharge and erythema, mild oedema and mild corneal cloudiness developed in all treated eyes 1 hour. Iris clarity improved overnight and discharge had nearly ceased within 3 days. A slight degree of redness and oedema remained after 7 days. The free acid was considered to be a moderate eye irritant in rabbits Result: under conditions of the test. Skin Sensitisation Skin Sensitisation test with the notified chemical (SEAC Toxicology Group, 1981b) Guinea pig/ Dunkin-Hartley Species/strain: 10 (6 males, 4 females) Number of animals: Induction procedure:
4 NE 0 NE 5 NE 0 NE 6 NE 0 0 Conjunctiva r c d r c d r C d 1 1 0 NE 0 0 0 0 0 0 2 1 0 NE 1 0 0 0 0 0 3 1 1 NE 1 0 0 0 0 0 4 0 0 NE 0 0 0 NE NE 5 1 0 NE 0 0 0 NE NE 6 1 0 NE 1 0 0 0 0 0 1 see Attachment 1 for Draize scales O = opacity, a = area, r = redness, c = chemosis, d = discharge, NE = Not estimated Result: The notified chemical was slightly irritating to the eyes of rabbits. Eye irritation with free acid (Younger Laboratories, 1968b) Species/strain: New Zealand White rabbit Number/sex of animals: 3 (2 males, 1 female) Observation period: 1 to 7 days after treatment Method of administration: 100 mg of finely ground free acid powder were placed into the conjunctival sac of the right eye of each rabbit. The eyes were examined for signs of irritation after 1, 24, 48, 72, 120 and 168 hours. The treated eyes were rinsed with warm isotonic saline 24 hours after treatment later. After the reading, fluorescein (concentration not stated) was instilled to detect corneal damage. Test method: Not stated Comment: Treated eyes were graded according to the severity of erythema, oedema, discharge, corneal opacity and iridial reactions on an arbitrary scale of 0 to 110. Moderate discharge and erythema, mild oedema and mild corneal cloudiness developed in all treated eyes 1 hour. Iris clarity improved overnight and discharge had nearly ceased within 3 days. A slight degree of redness and oedema remained after 7 days. Result: The free acid was considered to be a moderate eye irritant in rabbits under conditions of the test. Skin Sensitisation Skin Sensitisation test with the notified chemical (SEAC Toxicology Group, 1981b) Species/strain: Guinea pig/ Dunkin-Hartley Number of animals: 10 (6 males, 4 females) Induction procedure:
STD1016FR.docx