Repeat 14-15 contaminating with all other residue. samples. Disposable When you are plastic finished, clean the cuvettes can cuvettes by first be used a few emptying them, times before then flushing with they become several changes of scratched or purified water. stained and Next, flush with need to be several changes of discarded. 70-95% ethanol. Clean these Finally, dry upside carefully and down on a paper they will last towel before storing for several in a safe place. uses. Quartz If you are the last to cuvettes look use the like glass and spectrophotometer, are made to power off the have a long instrument. life. Treat these with special care PART B: DNA ANALYSIS because they BY SCANNING are very expensive. From the main They will last menu, highlight many years if Acid Tests” used carefully. “Nucleic and press Enter. Select “DNA with Scan 260/230”. Parameters will display; press the softkey labeled Test”. “Run Insert a cuvette filled with the blank solution appropriate to your experiment into the sample holder. Press the softkey labeled “Collect Baseline” and allow
Repeat 14-15 with all other samples. When you are finished, clean the cuvettes by first emptying them, then flushing with several changes of purified water. Next, flush with several changes of 70-95% ethanol. Finally, dry upside down on a paper towel before storing in a safe place. If you are the last to use the spectrophotometer, power off the instrument. PART B: DNA ANALYSIS BY SCANNING From the main menu, highlight “Nucleic Acid Tests” and press Enter. Select “DNA with Scan 260/230”. Parameters will display; press the softkey labeled “Run Test”. Insert a cuvette filled with the blank solution appropriate to your experiment into the sample holder. Press the softkey labeled “Collect Baseline” and allow contaminating residue. Disposable plastic cuvettes can be used a few times before they become scratched or stained and need to be discarded. Clean these carefully and they will last for several uses. Quartz cuvettes look like glass and are made to have a long life. Treat these with special care because they are very expensive. They will last many years if used carefully.
2012_fall_sop_book_9th_ed_1.docx