Remarks Results The test item had a statistically significant inhibitory effect on the growth – (biomass and growth rate) of after the Scenedesmus subspicatus exposures period of 72 hours at concentrations of 0.11 mg/L and above. The notified chemical is very toxic to algae. RCC (2002r) 8.2.4. Inhibition of microbial activity Sanitized T99-19 OECD TG 209 Activated Sludge, Respiration Inhibition Test. EC Directive 88/302/EEC C.11 Biodegradation: Activated Sludge Respiration Inhibition Test. Inoculum Synthetic sewage Exposure Period 3 Concentration 10, 32, 100, 320 and 1000 mg/L Nominal Remarks Method The rate of respiration was determined after 30 minutes and 3 hours – contact time and compared to data for the control and a reference material, 3,5-dichlorophenol. IC50 140 mg/L NOEC 32 mg/L Remarks Results Concentrations of 320 and 1000mg/L the test material was observed to – cause foaming. The validation criteria for the control respiration rates and reference material EC50 values have been satisfied. The notified chemical is not harmful to sewage microbes. Safepharm (2000e) 9. RISK ASSESSMENT 9.1. Environment 9.1.1. Environment exposure assessment – The majority of imported notified chemical is expected to be disposed of to landfill, predominantly associated with the end-use items to which it is affixed. In landfill, the notified chemical is expected to degrade by biotic and abiotic means to form simple carbon, nitrogen and silicon based compounds. It is anticipated that up to 3% may be released to sewer from spent dye-bath during use. In order to provide a conservative estimate, a fixation rate of 90% is assumed thus for the purposes of the following calculation 10% of the notified chemical is assumed to be released to the sewer during cleaning. The notifier has provided the following calculations.
Remarks – Results The test item had a statistically significant inhibitory effect on the growth (biomass and growth rate) of Scenedesmus subspicatus after the exposures period of 72 hours at concentrations of 0.11 mg/L and above. The notified chemical is very toxic to algae. RCC (2002r) 8.2.4. Inhibition of microbial activity Sanitized T99-19 OECD TG 209 Activated Sludge, Respiration Inhibition Test. EC Directive 88/302/EEC C.11 Biodegradation: Activated Sludge Respiration Inhibition Test. Inoculum Synthetic sewage Exposure Period 3 Concentration Nominal 10, 32, 100, 320 and 1000 mg/L Remarks – Method The rate of respiration was determined after 30 minutes and 3 hours contact time and compared to data for the control and a reference material, 3,5-dichlorophenol. IC50 140 mg/L NOEC 32 mg/L Remarks – Results Concentrations of 320 and 1000mg/L the test material was observed to cause foaming. The validation criteria for the control respiration rates and reference material EC50 values have been satisfied. The notified chemical is not harmful to sewage microbes. Safepharm (2000e) 9. RISK ASSESSMENT 9.1. Environment 9.1.1. Environment – exposure assessment The majority of imported notified chemical is expected to be disposed of to landfill, predominantly associated with the end-use items to which it is affixed. In landfill, the notified chemical is expected to degrade by biotic and abiotic means to form simple carbon, nitrogen and silicon based compounds. It is anticipated that up to 3% may be released to sewer from spent dye-bath during use. In order to provide a conservative estimate, a fixation rate of 90% is assumed thus for the purposes of the following calculation 10% of the notified chemical is assumed to be released to the sewer during cleaning. The notifier has provided the following calculations.
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