I wish to thank my supervisor Dr. R.A. Crowson for his helpful suggestions and corrections. KRICHER, JOHN C. BIOLOGY DEPARTMENT WHEATON COLLEGE NORTON, MASSACHUSETTS Effects of on the mortality of Tribolium confusum Introduction Numerous studies have been performed on the effects of pesticides on flour beetles. Reported here is a simple experiment which can be used to demonstrate the establishment of an LD 50, the effect of a pesticide on reproduction, and pesticides as agents of selection. Methods Tribolium confusum were cultured in 95% unbleached whole wheat flour plus 5% yeast at 29 0C and 30% relative humidity. Experimental and control cultures consisted of 30 adult T.confusum in 50 g of medium in a 225 ml. glass jar. Three replicates each were established for the control and three experimental. Experimental cultures contained 1$, 5% or 10% powdered Sevin (carbaryl) by weight. The pesticide was mixed evenly into the flour and yeast medium. Dead beetles were removed at each census and only live beetles were counted. Results By the end of 24 hours, distinct differences existed among the cultures containing differing concentrations of Sevin (Table 1).
I wish to thank my supervisor Dr. R.A. Crowson for his helpful suggestions and corrections. KRICHER, JOHN C. BIOLOGY DEPARTMENT WHEATON COLLEGE NORTON, MASSACHUSETTS Effects of on the mortality of Tribolium confusum Introduction Numerous studies have been performed on the effects of pesticides on flour beetles. Reported here is a simple experiment which can be used to demonstrate the establishment of an LD 50, the effect of a pesticide on reproduction, and pesticides as agents of selection. Methods Tribolium confusum were cultured in 95% unbleached whole wheat flour plus 5% yeast at 29 0C and 30% relative humidity. Experimental and control cultures consisted of 30 adult T.confusum in 50 g of medium in a 225 ml. glass jar. Three replicates each were established for the control and three experimental. Experimental cultures contained 1$, 5% or 10% powdered Sevin (carbaryl) by weight. The pesticide was mixed evenly into the flour and yeast medium. Dead beetles were removed at each census and only live beetles were counted. Results By the end of 24 hours, distinct differences existed among the cultures containing differing concentrations of Sevin (Table 1).
TIB_18.docx