Supplementary Figure 1. (a) Bactericidal efficacy of NTP on Staphylococcus aureus and Pseudomonas aeruginosa, expressed as log reduction after 15, 30 and 60 s of NTP treatment. Mean ± SEM, n = 3. (b) Scanning electron micrographs of untreated (control) and bacterial strains. References: 1 Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. 2001; 14: 244-69. Clin Microbiol Rev 2 Rahim K, Saleha S, Zhu X Bacterial Contribution in Chronicity of Wounds. et al. 2016: 1–12. Microb Ecol 3 Piddock LJV. The crisis of no new antibiotics-what is the way forward? Lancet Infect 2012; 12: 249-53. Dis 4 Fridman G, Friedman G, Gutsol A Applied plasma medicine. et al. Plasma Process 2008; 5: 503-33. Polym 5 Lunov O, Zablotskii V, Churpita O The interplay between biological and et al. physical scenarios of bacterial death induced by non-thermal plasma. Biomaterials 2016; 82: 71-83. Supplementary Table 1: TagMan® Gene Expression Assays gene Catalog no. Glyceraldehyde 3-phosphate dehydrogenase Rn01775763_g1 Gapdh Interleukin 2 Rn00587673_m1 Il2 Interleukin 6 Rn01410330_m1 Il6 Interleukin 10 Rn 00563409_m1 Il10 Chemokine (C-C motif) ligand 3 Rn01464736_g1 Ccl3 Chemokine (C-C motif) ligand 5 Rn00579590_m1 Ccl5 Prostaglandin-endoperoxide synthase 2 Rn01483828_m1 Ptgs2 Nitric oxide synthase 2, inducible Rn00561646_m1 Nos2 Epidermal growth factor Rn00563336_m1 Egf Connective tissue growth factor Rn01537279_g1 Ctgf CD86 molecule Rn00571654_m1 Cd86 CD163 molecule Rn01492519_m1 Cd163 Matrix metallopeptidase 2 Rn01538170_m1 Mmp2
Supplementary Figure 1. (a) Bactericidal efficacy of NTP on Staphylococcus aureus and Pseudomonas aeruginosa, expressed as log reduction after 15, 30 and 60 s of NTP treatment. Mean ± SEM, n = 3. (b) Scanning electron micrographs of untreated (control) and bacterial strains. References: 1 Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001; 14: 244-69. 2 Rahim K, Saleha S, Zhu X et al. Bacterial Contribution in Chronicity of Wounds. Microb Ecol 2016: 1–12. 3 Piddock LJV. The crisis of no new antibiotics-what is the way forward? Lancet Infect Dis 2012; 12: 249-53. 4 Fridman G, Friedman G, Gutsol A et al. Applied plasma medicine. Plasma Process Polym 2008; 5: 503-33. 5 Lunov O, Zablotskii V, Churpita O et al. The interplay between biological and physical scenarios of bacterial death induced by non-thermal plasma. Biomaterials 2016; 82: 71-83. Supplementary Table 1: TagMan® Gene Expression Assays gene Catalog no. Gapdh Glyceraldehyde 3-phosphate dehydrogenase Rn01775763_g1 Il2 Interleukin 2 Rn00587673_m1 Il6 Interleukin 6 Rn01410330_m1 Il10 Interleukin 10 Rn 00563409_m1 Ccl3 Chemokine (C-C motif) ligand 3 Rn01464736_g1 Ccl5 Chemokine (C-C motif) ligand 5 Rn00579590_m1 Ptgs2 Prostaglandin-endoperoxide synthase 2 Rn01483828_m1 Nos2 Nitric oxide synthase 2, inducible Rn00561646_m1 Egf Epidermal growth factor Rn00563336_m1 Ctgf Connective tissue growth factor Rn01537279_g1 Cd86 CD86 molecule Rn00571654_m1 Cd163 CD163 molecule Rn01492519_m1 Mmp2 Matrix metallopeptidase 2 Rn01538170_m1
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