Species Daphnia magna (Crustacea) Exposure Period 48 hours Auxiliary Solvent Not reported Water Hardness 154 mg CaCO3/L Analytical Monitoring Not reported Remarks Method less than 24 hours old, from a laboratory culture were Daphnia magna, – exposed to the test item. No information was provided on the test concentrations. Test conditions included: temperature 18–22ºC; pH total hardness 154 mg CaCO3/L; dissolved oxygen 8.6 9.0 mg/L. – No further information was provided on the test methods or procedures. LC50 > 1000 mg/L at 48 hours NOEC (or LOEC) Not reported. Remarks Results No further information on the test results was provided. – The test substance is very slightly toxic to (Mensink Daphnia magna et 1995). al. ECB (2000) 8.2.3. Algal growth inhibition test Fluid (DOT 4 type) No method description provided. Species (Algae) Selenastrum capricornutum Exposure Period 96 hours Concentration Range Not specified Nominal Concentration Not specified Actual Auxiliary Solvent Not specified Water Hardness Not specified Analytical Monitoring Not specified Remarks Method Test parameters were: temperature 22–26ºC; pH 7.0–7.4; initial – concentration of 500 cells/mL; illumination ~3000 lux; cell counts were made using a Coulter Counter. No further information was provided on the test methods or procedures. Remarks Results EC50 (growth rate), 430 mg/L at 96 h. No further information on the test – results was provided. The test substance is very slightly toxic to green algae (Mensink et al. 1995). ECB (2000) 8.2.4.1 Inhibition of microbial activity Borate Ester B DIN EN ISO 11348-2 (1999) Luminescent bacteria test – Inoculum (Bacteria) Vibrio fischeri Exposure Period 30 minutes Concentration Range Nine test concentrations in the range from 0.3 to 5.1 g/L, using dilution Nominal ratios of 1:2, 1:3, 1:4, 1:6, 1:8, 1:12, 1:16, 1:24, 1:32.
Species Daphnia magna (Crustacea) Exposure Period 48 hours Auxiliary Solvent Not reported Water Hardness 154 mg CaCO3/L Analytical Monitoring Not reported Remarks – Method Daphnia magna, less than 24 hours old, from a laboratory culture were exposed to the test item. No information was provided on the test concentrations. Test conditions included: temperature 18–22ºC; pH total hardness 154 mg CaCO3/L; dissolved oxygen 8.6 – 9.0 mg/L. No further information was provided on the test methods or procedures. LC50 > 1000 mg/L at 48 hours NOEC (or LOEC) Not reported. Remarks – Results No further information on the test results was provided. The test substance is very slightly toxic to Daphnia magna (Mensink et al. 1995). ECB (2000) 8.2.3. Algal growth inhibition test Fluid (DOT 4 type) No method description provided. Species Selenastrum capricornutum (Algae) Exposure Period 96 hours Concentration Range Nominal Not specified Concentration Actual Not specified Auxiliary Solvent Not specified Water Hardness Not specified Analytical Monitoring Not specified Remarks – Method Test parameters were: temperature 22–26ºC; pH 7.0–7.4; initial concentration of 500 cells/mL; illumination ~3000 lux; cell counts were made using a Coulter Counter. No further information was provided on the test methods or procedures. Remarks – Results EC50 (growth rate), 430 mg/L at 96 h. No further information on the test results was provided. The test substance is very slightly toxic to green algae (Mensink et al. 1995). ECB (2000) 8.2.4.1 Inhibition of microbial activity Borate Ester B DIN EN ISO 11348-2 (1999) – Luminescent bacteria test Inoculum Vibrio fischeri (Bacteria) Exposure Period 30 minutes Concentration Range Nominal Nine test concentrations in the range from 0.3 to 5.1 g/L, using dilution ratios of 1:2, 1:3, 1:4, 1:6, 1:8, 1:12, 1:16, 1:24, 1:32.
STD1024FR.docx