Since the test substance did not reach 60% degradation it cannot be classified as readily biodegradable. EBS 8.1.2. Bioaccumulation Not determined. The notified chemical has the potential to bioaccumulate but this is unlikely due to its low environmental exposure. 8.2. Ecotoxicological investigations 8.2.1. toxicity to fish Analogous chemical. OECD TG 203 Fish, Acute Toxicity Test semi-static. – EC Directive 92/69/EEC C.1 Acute Toxicity for Fish - semi-static. Species Rainbow trout (Oncorhynchus mykiss) Exposure Period 96 hours Auxiliary Solvent None. Water Hardness 150 mg CaCO3/L Analytical Monitoring Total Organic Carbon (TOC) Remarks Method The test material was prepared as a Accommodated Fraction (WAF) – due to its expected low water solubility. The mixtures were stirred at room temperature for 24 h and allowed to settle for 1 h. Following the settling period the water phase containing the WAF was removed from the bottom outlet of the vessel. Each concentration was tested in duplicate, with the renewed daily, using freshly prepared WAF. The test vessels were exposed to 16 hours of light and 8 hours of dark. The dissolved oxygen, pH and temperature were measured before and after media renewal. These environmental parameters all varied within acceptable limits. Concentration mg/L (WAF) Number of Fish Mortality Nominal Actual 0 h 24 h 48 h 72 h 96 h 0 10 0 0 0 0 0 100 10 0 0 0 0 0 1000 10 0 0 0 0 0 LL50 >1000 mg/L (WAF) at 96 hours. NOEL 1000 mg/L (WAF) at 96 hours. Remarks Results The TOC analysis indicated that the total organic carbon content of the – WAFs was below the quantification limit of 1 ppm. Under the study conditions the test substance is not toxic to fish, up to the limit of its water solubility. EBS (1996j) 8.2.2. Acute toxicity to aquatic invertebrates Analogous chemical. OECD TG 202 Daphnia sp. Acute Immobilisation Test static test. –
Since the test substance did not reach 60% degradation it cannot be classified as readily biodegradable. EBS 8.1.2. Bioaccumulation Not determined. The notified chemical has the potential to bioaccumulate but this is unlikely due to its low environmental exposure. 8.2. Ecotoxicological investigations 8.2.1. toxicity to fish Analogous chemical. OECD TG 203 Fish, Acute Toxicity Test – semi-static. EC Directive 92/69/EEC C.1 Acute Toxicity for Fish - semi-static. Species Rainbow trout (Oncorhynchus mykiss) Exposure Period 96 hours Auxiliary Solvent None. Water Hardness 150 mg CaCO3/L Analytical Monitoring Total Organic Carbon (TOC) Remarks – Method The test material was prepared as a Accommodated Fraction (WAF) due to its expected low water solubility. The mixtures were stirred at room temperature for 24 h and allowed to settle for 1 h. Following the settling period the water phase containing the WAF was removed from the bottom outlet of the vessel. Each concentration was tested in duplicate, with the renewed daily, using freshly prepared WAF. The test vessels were exposed to 16 hours of light and 8 hours of dark. The dissolved oxygen, pH and temperature were measured before and after media renewal. These environmental parameters all varied within acceptable limits. Concentration mg/L (WAF) Number of Fish Mortality Nominal Actual 0 h 24 h 48 h 72 h 96 h 0 10 0 0 0 0 0 100 10 0 0 0 0 0 1000 10 0 0 0 0 0 LL50 >1000 mg/L (WAF) at 96 hours. NOEL 1000 mg/L (WAF) at 96 hours. Remarks – Results The TOC analysis indicated that the total organic carbon content of the WAFs was below the quantification limit of 1 ppm. Under the study conditions the test substance is not toxic to fish, up to the limit of its water solubility. EBS (1996j) 8.2.2. Acute toxicity to aquatic invertebrates Analogous chemical. OECD TG 202 Daphnia sp. Acute Immobilisation Test – static test.
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