Remarks Method No significant protocol deviations were reported except that due to the – coloured nature of the test substance an increase light intensity and decreased test volume was used. Biomass Growth NOEC ErC50 NOEC mg/L at 72 h mg/L mg/L at 0-72 h mg/L >100 100 >100 100 Remarks Results Analysis of the test preparations at 0 and 72 hours showed measured test – concentrations to range from 99% to 105% of nominal and so the results are based on nominal test concentrations only. The notified chemical is not harmful to Scenedesmus subspicatus. Safepharm Laboratories Limited (2006p) growth inhibition test Lemna minor Notified chemical. OECD Guideline Growth Inhibition Test (April 2004)”, “Lemna modified for testing coloured test substances. Species Lemna minor Exposure Period 7 days. Concentration Range 0 and 100 mg/L Nominal Concentration Range 0 and 102-106 mg/L Actual Auxiliary Solvent Culture medium Water Hardness Not applicable as the substance was dissolved in culture medium. Analytical Monitoring No significant protocol deviations were reported. Remarks Method None. – Biomass Growth EC50 NOEC EC50 NOEC Average Specific Growth Average Specific Growth Yield Yield Rate Rate (frond number and dry (frond number and dry number and dry number and dry weight) weight) weight) weight) mg/L at 72 h mg/L mg/L at 0-72 h mg/L >100 100 >100 100 Remarks Results Analysis of the test preparations on days 0 (fresh media) and Day 2, 4 – and 7 (old media) showed measured test concentrations to range from 102% to 106% of nominal and hence the results are based on nominal test concentrations only. The notified chemical is not harmful to Lemna minor. Safepharm Laboratories Limited (2006q) Inhibition of microbial activity
Remarks – Method No significant protocol deviations were reported except that due to the coloured nature of the test substance an increase light intensity and decreased test volume was used. Biomass Growth NOEC ErC50 NOEC mg/L at 72 h mg/L mg/L at 0-72 h mg/L >100 100 >100 100 Remarks – Results Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to range from 99% to 105% of nominal and so the results are based on nominal test concentrations only. The notified chemical is not harmful to Scenedesmus subspicatus. Safepharm Laboratories Limited (2006p) Lemna minor growth inhibition test Notified chemical. OECD Guideline “Lemna Growth Inhibition Test (April 2004)”, modified for testing coloured test substances. Species Lemna minor Exposure Period 7 days. Concentration Range Nominal 0 and 100 mg/L Concentration Range Actual 0 and 102-106 mg/L Auxiliary Solvent Culture medium Water Hardness Not applicable as the substance was dissolved in culture medium. Analytical Monitoring No significant protocol deviations were reported. Remarks – Method None. Biomass Growth EC50 Average Specific Growth Rate number and dry weight) NOEC Average Specific Growth Rate number and dry weight) EC50 Yield (frond number and dry weight) NOEC Yield (frond number and dry weight) mg/L at 72 h mg/L mg/L at 0-72 h mg/L >100 100 >100 100 Remarks – Results Analysis of the test preparations on days 0 (fresh media) and Day 2, 4 and 7 (old media) showed measured test concentrations to range from 102% to 106% of nominal and hence the results are based on nominal test concentrations only. The notified chemical is not harmful to Lemna minor. Safepharm Laboratories Limited (2006q) Inhibition of microbial activity
STD-1267-FR.docx