printout of your results. SOP SPC-003 NANODROP 1000 SPECTROPHOTOMETER Operation of NanoDrop 1000 Spectrophotometer Title: 1.0 SCOPE AND APPLICATION: This SOP outlines the policy and procedure for operation of the NanoDrop 1000 Spectrophotometer. 2.0 SUMMARY OF METHOD MATERIALS NanoDrop 1000 spectrophotometer IMPORTANT: do not handle the optic Attached computer with ND1000 cable on the NanoDrop. This can damage software it and cause problems with the instrument. Kimwipes, DI water, p2 pipette When lifting and lowering the pedestal, handle only by the metal part at the PART A: OPERATION bottom. Keep the instrument away from objects that could fall on or snag the cable to prevent damage. Open the ND1000 software on the attached computer and select the analysis module of interest (nucleic acid, protein, etc). Follow prompts to initialize the spectrophotometer by pipetting 2 uL of DI onto the bottom pedestal and clicking OK. When this is finished, wipe the water off with a kimwipe. Establish a reference or blank using the appropriate buffer. Pipette 1-2 µL of blanking buffer onto the bottom
printout of your results. SOP SPC-003 NANODROP 1000 SPECTROPHOTOMETER Title: Operation of NanoDrop 1000 Spectrophotometer 1.0 SCOPE AND APPLICATION: This SOP outlines the policy and procedure for operation of the NanoDrop 1000 Spectrophotometer. 2.0 SUMMARY OF METHOD MATERIALS NanoDrop 1000 spectrophotometer Attached computer with ND1000 software Kimwipes, DI water, p2 pipette PART A: OPERATION Open the ND1000 software on the attached computer and select the analysis module of interest (nucleic acid, protein, etc). Follow prompts to initialize the spectrophotometer by pipetting 2 uL of DI onto the bottom pedestal and clicking OK. When this is finished, wipe the water off with a kimwipe. Establish a reference or blank using the appropriate buffer. Pipette 1-2 µL of blanking buffer onto the bottom IMPORTANT: do not handle the optic cable on the NanoDrop. This can damage it and cause problems with the instrument. When lifting and lowering the pedestal, handle only by the metal part at the bottom. Keep the instrument away from objects that could fall on or snag the cable to prevent damage.
2012_fall_sop_book_9th_ed_1.docx