PART A: SINGLE instrument are WAVELENGTH optically READINGS The light path Turn on the travels from spectrophotometer right to left by pressing the across the power button. instrument. It is best to Wait for the handle instrument to cuvettes by initialize and the optically complete internal opaque tests. The default to avoid home page will be leaving any displayed. residue on the clear sides. Press 1 on the number keypad to choose Single Wavelength. the wavelength you would like to use, then press the green button on ► the keypad to accept. The blank solution should Insert a cuvette be identical to containing the or as similar as appropriate possible to the solution. Press the solution in 0A/100%T button which the on the keypad to compound to take a blank be measured reading. is suspended. This is usually Remove the blank water or an cuvette and insert aqueous a cuvette buffer. containing the sample. Press the green button to ► take a reading. The absorbance will be A 1/10 dilution displayed. is a good If the absorbance place to start. value of a sample is Use the blank above the range of solution to the instrument, you dilute the
PART A: SINGLE WAVELENGTH READINGS Turn on the spectrophotometer by pressing the power button. Wait for the instrument to initialize and complete internal tests. The default home page will be displayed. Press 1 on the number keypad to choose Single Wavelength. the wavelength you would like to use, then press the green ► button on the keypad to accept. Insert a cuvette containing the appropriate solution. Press the 0A/100%T button on the keypad to take a blank reading. Remove the blank cuvette and insert a cuvette containing the sample. Press the green ► button to take a reading. The absorbance will be displayed. If the absorbance value of a sample is above the range of the instrument, you instrument are optically The light path travels from right to left across the instrument. It is best to handle cuvettes by the optically opaque to avoid leaving any residue on the clear sides. The blank solution should be identical to or as similar as possible to the solution in which the compound to be measured is suspended. This is usually water or an aqueous buffer. A 1/10 dilution is a good place to start. Use the blank solution to dilute the
2012_fall_sop_book_9th_ed_1.docx