Notified chemical The potential of the notified chemical to cause serious damage to the eye/mucous membranes was assessed by a single topic application of 0.3 mL of the undiluted test substance and 0.3 mL of a 10% test substance solution in olive oil to the chorionallantoic membrane () of fertilised and incubated hen eggs. Three eggs per test substance concentration were observed until unambiguous irritation reactions were detected. The occurrence of vascular injury or intravascular coagulation in response to the test substance was recorded. Concentration: Egg-No.: Time (seconds) until appearance of: Grading of effects: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: Undiluted test 1 64 64 1 2 substance 2 53 53 1 2 3 51 51 1 2 Mean: n = 3 56 56 1 2 Concentration: Egg-No.: Time (seconds) until Grading of effects: appearance of: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: 10% test 1 30 30 1 2 substance in 2 36 36 1 2 oil 3 41 41 1 2 Mean: n = 3 36 36 1 2 Positive controls Concentration: Egg-No.: Time (seconds) until appearance Grading of effects: of: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: 0.1 M NaOH 1 20 40 2 2 2 28 42 2 2 Mean: n = 2 24 41 2 2 Concentration: Egg-No.: Time (seconds) until Grading of effects: appearance of: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: 10% sodium 1 22 41 2 2 dodecyl sulfate 2 21 35 2 2 Mean: n = 2 22 38 2 2 Remarks - Results The undiluted test substance caused moderate (grade 2) intravascular coagulation and slight (grade 1) haemorrhagia in all eggs after 51-64 seconds. The 10% test substance solution in olive oil caused moderate intravascular coagulation and slight haemorrhagia in all eggs after 30-41 seconds. The notified chemical is severely irritating to the eye the test conditions. BASF SE (2009f) - Skin sensitisation local lymph node assay (LLNA) – Notified chemical
Notified chemical The potential of the notified chemical to cause serious damage to the eye/mucous membranes was assessed by a single topic application of 0.3 mL of the undiluted test substance and 0.3 mL of a 10% test substance solution in olive oil to the chorionallantoic membrane () of fertilised and incubated hen eggs. Three eggs per test substance concentration were observed until unambiguous irritation reactions were detected. The occurrence of vascular injury or intravascular coagulation in response to the test substance was recorded. Concentration: Egg-No.: Time (seconds) until appearance of: Grading of effects: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: Undiluted test substance 1 64 64 1 2 2 53 53 1 2 3 51 51 1 2 Mean: n = 3 56 56 1 2 Concentration: Egg-No.: Time (seconds) until appearance of: Grading of effects: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: 10% test substance in oil 1 30 30 1 2 2 36 36 1 2 3 41 41 1 2 Mean: n = 3 36 36 1 2 Positive controls Concentration: Egg-No.: Time (seconds) until appearance of: Grading of effects: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: 0.1 M NaOH 1 20 40 2 2 2 28 42 2 2 Mean: n = 2 24 41 2 2 Concentration: Egg-No.: Time (seconds) until appearance of: Grading of effects: Haemorrhagia: Coagulation: Haemorrhagia: Coagulation: 10% sodium dodecyl sulfate 1 22 41 2 2 2 21 35 2 2 Mean: n = 2 22 38 2 2 Remarks - Results The undiluted test substance caused moderate (grade 2) intravascular coagulation and slight (grade 1) haemorrhagia in all eggs after 51-64 seconds. The 10% test substance solution in olive oil caused moderate intravascular coagulation and slight haemorrhagia in all eggs after 30-41 seconds. The notified chemical is severely irritating to the eye the test conditions. BASF SE (2009f) - Skin sensitisation – local lymph node assay (LLNA) Notified chemical
STD1384FR.docx