From the home screen, the softkey at the Some plastics bottom right are not labeled “Basic ATC.” and not be useful for Press the softkey UV labeled nm.” absorbance “Set Enter the desired readings. wavelength using the numeric The blank keypad and press solution should Enter. be identical to or as similar as Choose cuvettes possible to the appropriate for the solution in wavelength. Make which the sure they are clean compound to on the inside and be measured outside and free is suspended. from scratches or This is usually stains. water or an aqueous Wearing gloves, fill buffer. one clean cuvette at least halfway Orient the with the solution to cuvette such be used as a blank. that the optically Fill another cuvette sides are at least halfway aligned with with the solution to the light path. be tested for absorbance. If It is best to there is a shortage handle of cuvettes, the cuvettes by blank can be the optically measured first, then opaque sides removed, and the to avoid blank solution leaving any replaced with the residue on the solution to be clear sides. tested. Carefully wipe the optically clear sides of the cuvettes with a Kimwipe or lens paper to remove any drops of liquid, dust, or fingerprints.
From the home screen, the softkey at the bottom right labeled “Basic ATC.” Press the softkey labeled “Set nm.” Enter the desired wavelength using the numeric keypad and press Enter. Choose cuvettes appropriate for the wavelength. Make sure they are clean on the inside and outside and free from scratches or stains. Wearing gloves, fill one clean cuvette at least halfway with the solution to be used as a blank. Fill another cuvette at least halfway with the solution to be tested for absorbance. If there is a shortage of cuvettes, the blank can be measured first, then removed, and the blank solution replaced with the solution to be tested. Carefully wipe the optically clear sides of the cuvettes with a Kimwipe or lens paper to remove any drops of liquid, dust, or fingerprints. Some plastics are not and not be useful for UV absorbance readings. The blank solution should be identical to or as similar as possible to the solution in which the compound to be measured is suspended. This is usually water or an aqueous buffer. Orient the cuvette such that the optically sides are aligned with the light path. It is best to handle cuvettes by the optically opaque sides to avoid leaving any residue on the clear sides.
2012_fall_sop_book_9th_ed_1.docx