for the cell sensitive biological culture and harvesting as well as the reasonable bubble size generated work well in the mixing process in the bioreactors. Therefore, it is important and essential to choose a suitable generation method depending on the treatment application. As mentioned previously, microbubble size is the essential key in controlling the performance in all the application. the bubble size generated from different microbubble generation method should be studied in order to choose a suitable bubble generation method for the desired bubble required to improve the efficiency of the application. 2.4 Bubble Size Measurement Techniques 2.4.1 Introduction This section mainly illustrates the and cons’ of the two bubble size measurement ‘pros methods. The two methods are discussed in this chapter which are optical (photography method) and photonic method (Spraytec). This chapter illustrates the working principle, using procedure, specifications and the analysis methods of all the bubble size measurement methods. 2.4.2 Optical Methods Technique –Photography Figure 2.21: Schematic diagram of the optical method experimental setup 2.4.2.1 Specification and Working Principle The apparatus for the analysis system includes a camera (FastCam SA3 Model120K) and a 40 Watt halogen light with light source diffuser sheet (Caddilac). The camera resolution used was 1024 X 1024 pixels at 2000 fps and provided with a macro lens (Nikon Nikkor Lens, 1.8 D). The calibration scale factor measured 25.0
for the cell sensitive biological culture and harvesting as well as the reasonable bubble size generated work well in the mixing process in the bioreactors. Therefore, it is important and essential to choose a suitable generation method depending on the treatment application. As mentioned previously, microbubble size is the essential key in controlling the performance in all the application. the bubble size generated from different microbubble generation method should be studied in order to choose a suitable bubble generation method for the desired bubble required to improve the efficiency of the application. 2.4 Bubble Size Measurement Techniques 2.4.1 Introduction This section mainly illustrates the ‘pros and cons’ of the two bubble size measurement methods. The two methods are discussed in this chapter which are optical (photography method) and photonic method (Spraytec). This chapter illustrates the working principle, using procedure, specifications and the analysis methods of all the bubble size measurement methods. 2.4.2 Optical Methods –Photography Technique Figure 2.21: Schematic diagram of the optical method experimental setup 2.4.2.1 Specification and Working Principle The apparatus for the analysis system includes a camera (FastCam SA3 Model120K) and a 40 Watt halogen light with light source diffuser sheet (Caddilac). The camera resolution used was 1024 X 1024 pixels at 2000 fps and provided with a macro lens (Nikon Nikkor Lens, 1.8 D). The calibration scale factor measured 25.0
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