Concentration mg/L Number of Fish Mortality Nominal Actual 1 h 24 h 48 h 72 h 96 h 0 0 7 0 0 0 0 0 100 90-94 7 0 0 0 0 0 LC50 > 100 mg/L at 96 hours. NOEC 100 mg/L at 96 hours. Remarks Results In the control and in the 100 mg/L test, all fish survived until the end of – the test and no visible abnormalities were observed. No remarkable observations were made concerning the appearance of the test medium. The test medium was a clear solution throughout the whole test duration. The notified chemical was not harmful to fish at a concentration of 100 mg/L. RCC 8.2.2. Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test and Reproduction Test Static. – EC Directive 92/69/EEC C.2 Acute Toxicity for Daphnia Static. – Species Daphnia Exposure Period 48 Auxiliary Solvent None Water Hardness 250 mg Analytical Monitoring HPLC Remarks - Method The test medium was prepared by dissolving 30.2 mg of test item completely in 300 mL of test water by intense stirring for 15 minutes at room temperature. No significant deviations from the test guidelines were reported. Concentration mg/L Number of D. magna Number Immobilised Nominal Actual 24 h 48 h 0 0 20 0 0 100 98-112 20 0 0 LC50 > 100 mg/L at 48 hours NOEC 100 mg/L at 48 hours Remarks - Results The test medium was a clear solution throughout the duration of the test. The notified chemical was not harmful to daphnids at a concentration of 100 mg/L. RCC (2006f) 8.2.3. Algal growth inhibition test Notified chemical OECD TG 201 Alga, Growth Inhibition Test. EC Directive 92/69/EEC C.3 Algal Inhibition Test. Species Scenedesmus subspicatus Exposure Period 72 hours
Concentration mg/L Number of Fish Mortality Nominal Actual 1 h 24 h 48 h 72 h 96 h 0 0 7 0 0 0 0 0 100 90-94 7 0 0 0 0 0 LC50 > 100 mg/L at 96 hours. NOEC 100 mg/L at 96 hours. Remarks – Results In the control and in the 100 mg/L test, all fish survived until the end of the test and no visible abnormalities were observed. No remarkable observations were made concerning the appearance of the test medium. The test medium was a clear solution throughout the whole test duration. The notified chemical was not harmful to fish at a concentration of 100 mg/L. RCC 8.2.2. Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test and Reproduction Test – Static. EC Directive 92/69/EEC C.2 Acute Toxicity for Daphnia – Static. Species Daphnia Exposure Period 48 Auxiliary Solvent None Water Hardness 250 mg Analytical Monitoring HPLC Remarks - Method The test medium was prepared by dissolving 30.2 mg of test item completely in 300 mL of test water by intense stirring for 15 minutes at room temperature. No significant deviations from the test guidelines were reported. Concentration mg/L Number of D. magna Number Immobilised Nominal Actual 24 h 48 h 0 0 20 0 0 100 98-112 20 0 0 LC50 > 100 mg/L at 48 hours NOEC 100 mg/L at 48 hours Remarks - Results The test medium was a clear solution throughout the duration of the test. The notified chemical was not harmful to daphnids at a concentration of 100 mg/L. RCC (2006f) 8.2.3. Algal growth inhibition test Notified chemical OECD TG 201 Alga, Growth Inhibition Test. EC Directive 92/69/EEC C.3 Algal Inhibition Test. Species Scenedesmus subspicatus Exposure Period 72 hours
STD1231FR.docx