Go back to the gas regulator. Look at the gauge on the left; it should register approximately 10 psi. If it is significantly above or below this value, CAREFULLY AND SLOWLY adjust the white valve until the pressure is between 9 and 11 psi. Allow the incubator to equilibrate to the set point temperature and pressure. The relative humidity inside the incubator will increase as the water from the reservoir evaporates into the warm air. No need to change any settings for humidity. After 10 minutes check the temperature and % CO2. If the temperature is too low, give the incubator some more time to equilibrate. Confirm the temperature using a certified thermometer or temperature probe. If the CO2 is too high or low, check the regulator gauge. The right gauge IMPORTANT: the CO2 inside the tank is should display several thousand PSI such high pressure that some of it is while there is gas in the tank (this liquefied. As gas is consumed and leaves cannot be adjusted). The left gauge the tank, the liquid amount grows smaller; should be user-adjusted to around 10 however, the same pressure will register on psi. the regulator as long as there is liquid CO2 in the tank. What this means is that you will When the temperature and % CO2 not observe a decrease in the pressure of have reached the set points, the gas in the tank UNTIL there is almost none humidity will also have equilibrated to left, then the pressure will rapidly decrease the appropriate level and cells can be (within the same day or so). Therefore, it is safely placed in the incubator. important that the tank is monitored and a spare tank is kept on-site so they can be Only open the interior door when you switched out quickly when the original tank need to add or remove items from the empties. incubator. Try to coordinate these openings with other users to minimize the total number of openings per day. When you open the interior door quickly remove or replace your item and close the door; do not leave the interior door open for any reason if the incubator is on. PART B: MAINTENANCE
Go back to the gas regulator. Look at the gauge on the left; it should register approximately 10 psi. If it is significantly above or below this value, CAREFULLY AND SLOWLY adjust the white valve until the pressure is between 9 and 11 psi. Allow the incubator to equilibrate to the set point temperature and pressure. The relative humidity inside the incubator will increase as the water from the reservoir evaporates into the warm air. No need to change any settings for humidity. After 10 minutes check the temperature and % CO2. If the temperature is too low, give the incubator some more time to equilibrate. Confirm the temperature using a certified thermometer or temperature probe. If the CO2 is too high or low, check the regulator gauge. The right gauge should display several thousand PSI while there is gas in the tank (this cannot be adjusted). The left gauge should be user-adjusted to around 10 psi. When the temperature and % CO2 have reached the set points, the humidity will also have equilibrated to the appropriate level and cells can be safely placed in the incubator. Only open the interior door when you need to add or remove items from the incubator. Try to coordinate these openings with other users to minimize the total number of openings per day. When you open the interior door quickly remove or replace your item and close the door; do not leave the interior door open for any reason if the incubator is on. PART B: MAINTENANCE IMPORTANT: the CO2 inside the tank is such high pressure that some of it is liquefied. As gas is consumed and leaves the tank, the liquid amount grows smaller; however, the same pressure will register on the regulator as long as there is liquid CO2 in the tank. What this means is that you will not observe a decrease in the pressure of gas in the tank UNTIL there is almost none left, then the pressure will rapidly decrease (within the same day or so). Therefore, it is important that the tank is monitored and a spare tank is kept on-site so they can be switched out quickly when the original tank empties.
2012_fall_sop_book_9th_ed_1.docx