8. ENVIRONMENT 8.1. Environmental fate 8.1.1. Ready biodegradability Notified chemical Testing methods for new chemical substances, Kanpogyo No.5, Planning and coordination Bureau, Environment Agency, Yakuhatu No. 615, Pharmaceutical Affairs Bureau, Ministry of Health and Welfare, and 49 Kikyoku No. 392, Basic Industries Bureau, Ministry of International Trade and Industry, Japan. Inoculum Mixture of activated sludge from 10 locations in Japan Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring HPLC and BOD Remarks - Method The concentration of the test substance used was 100 mg/L. The medium was inoculated with micro-organisms derived from the activated sludge. Five treatment groups were established in the system: a control consisting of sludge with reference (aniline), sludge with test substance (triplicate), water with the test substance and a blank. The % biodegradation of the test substance was measured by BOD and HPLC on days 7, 14, 21 and 28. The results of the BOD and HPLC determinations are shown below. Test substance Aniline Day % Degradation Day % Degradation HPLC BOD 7 6 0 7 59 14 6 0 14 71 21 4 0 21 73 28 6 0 28 74 Remarks - Results The test substance was neither soluble in water nor in sludge at the initiation of cultivation. At the completion of the cultivation, transformation was not observed compared with the initiation of cultivation for test substance in water. The test solutions were cloudy and the growth of the sludge was not observed at the completion of the cultivation. The test substance was stable during the cultivation based on the HPLC of the test substance before the start and after the termination of the cultivation. The reference substance underwent 74% biodegradation after 28 days thus validating the test. The test substance is considered to be not readily biodegradable. Kurume Research Laboratories 8.1.2. Inherent biodegradability Notified chemical OECD guideline 302D for testing of chemicals: ‘Inherent biodegradability Concawe test (October 2001), using a pre-exposed – inoculum.
8. ENVIRONMENT 8.1. Environmental fate 8.1.1. Ready biodegradability Notified chemical Testing methods for new chemical substances, Kanpogyo No.5, Planning and coordination Bureau, Environment Agency, Yakuhatu No. 615, Pharmaceutical Affairs Bureau, Ministry of Health and Welfare, and 49 Kikyoku No. 392, Basic Industries Bureau, Ministry of International Trade and Industry, Japan. Inoculum Mixture of activated sludge from 10 locations in Japan Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring HPLC and BOD Remarks - Method The concentration of the test substance used was 100 mg/L. The medium was inoculated with micro-organisms derived from the activated sludge. Five treatment groups were established in the system: a control consisting of sludge with reference (aniline), sludge with test substance (triplicate), water with the test substance and a blank. The % biodegradation of the test substance was measured by BOD and HPLC on days 7, 14, 21 and 28. The results of the BOD and HPLC determinations are shown below. Test substance Aniline Day % Degradation Day % Degradation HPLC BOD 7 6 0 7 59 14 6 0 14 71 21 4 0 21 73 28 6 0 28 74 Remarks - Results The test substance was neither soluble in water nor in sludge at the initiation of cultivation. At the completion of the cultivation, transformation was not observed compared with the initiation of cultivation for test substance in water. The test solutions were cloudy and the growth of the sludge was not observed at the completion of the cultivation. The test substance was stable during the cultivation based on the HPLC of the test substance before the start and after the termination of the cultivation. The reference substance underwent 74% biodegradation after 28 days thus validating the test. The test substance is considered to be not readily biodegradable. Kurume Research Laboratories 8.1.2. Inherent biodegradability Notified chemical OECD guideline 302D for testing of chemicals: ‘Inherent biodegradability – Concawe test (October 2001), using a pre-exposed inoculum.
STD1132FR.docx