default home these with page. special care because they Enter the start and are very end wavelengths expensive. and press the green They will last button. many years if ► used carefully. Insert a cuvette containing the appropriate solution. Press the 0A/100%T button on the keypad to take a blank reading. Remove the blank cuvette and insert a cuvette containing the sample. Press the green button to ► take a reading. The absorbance spectrum will be displayed. Repeat with any other samples. The blank solution should When you are be identical to finished, clean the or as similar as cuvettes by first possible to the emptying them, solution in then flushing with which the several changes of compound to purified water. be measured Next, flush with is suspended. several changes of This is usually 70-95% ethanol. water or an Finally, dry upside aqueous down on a paper buffer. towel before storing in a safe place. If you are the last to use the A 1/10 dilution spectrophotometer, is a good power off the place to start. instrument. Use the blank solution to
default home page. Enter the start and end wavelengths and press the green ► button. Insert a cuvette containing the appropriate solution. Press the 0A/100%T button on the keypad to take a blank reading. Remove the blank cuvette and insert a cuvette containing the sample. Press the green ► button to take a reading. The absorbance spectrum will be displayed. Repeat with any other samples. When you are finished, clean the cuvettes by first emptying them, then flushing with several changes of purified water. Next, flush with several changes of 70-95% ethanol. Finally, dry upside down on a paper towel before storing in a safe place. If you are the last to use the spectrophotometer, power off the instrument. these with special care because they are very expensive. They will last many years if used carefully. The blank solution should be identical to or as similar as possible to the solution in which the compound to be measured is suspended. This is usually water or an aqueous buffer. A 1/10 dilution is a good place to start. Use the blank solution to
2012_fall_sop_book_9th_ed_1.docx