menu(s). Click OK when finished with shake for 3-5 seconds, followed by a this Read step. delay before reading. Enter Delay, Temperature Set, Shake or more Read steps if desired. When you If you do not turn on the Tungsten lamp, have finished designing your the Xenon flash lamp will be used. The procedure, you may click Validate to Xenon lamp does not need to warm up make sure the order of your steps is before use. logical (this is only useful when you have entered more than one step). when finished designing your OK procedure. Enter a plate ID on the next menu and add comments if desired. Click Read. The Save As dialog box will open; give your data set a unique name and click Save. The sample tray will open. Insert your plate with well A1 oriented towards the upper left corner. Click OK, the tray will close, and the reader will scan the plate. The tray will open when the procedure No more than 350 µL should ever be is finished. Remove the plate and added to a microplate for use in this manually close the tray by pressing the instrument, due to the risk of spills. black eject button on the front panel of the instrument. A window with a schematic of the plate containing data will appear. You may need to choose the wavelength in the Data drop-down menu to see the data. To export data to Excel, click the Excel icon next to the Data drop-down menu. To read another plate with the same procedure, click the Add Plate icon on the toolbar. Follow the above directions, starting with entering a plate ID. PART B: MAINTENANCE Routine maintenance of the instrument should include cleaning the plate carrier to remove sample residue (an
menu(s). Click OK when finished with this Read step. Enter Delay, Temperature Set, Shake or more Read steps if desired. When you have finished designing your procedure, you may click Validate to make sure the order of your steps is logical (this is only useful when you have entered more than one step). OK when finished designing your procedure. Enter a plate ID on the next menu and add comments if desired. Click Read. The Save As dialog box will open; give your data set a unique name and click Save. The sample tray will open. Insert your plate with well A1 oriented towards the upper left corner. Click OK, the tray will close, and the reader will scan the plate. The tray will open when the procedure is finished. Remove the plate and manually close the tray by pressing the black eject button on the front panel of the instrument. A window with a schematic of the plate containing data will appear. You may need to choose the wavelength in the Data drop-down menu to see the data. To export data to Excel, click the Excel icon next to the Data drop-down menu. To read another plate with the same procedure, click the Add Plate icon on the toolbar. Follow the above directions, starting with entering a plate ID. PART B: MAINTENANCE Routine maintenance of the instrument should include cleaning the plate carrier to remove sample residue (an shake for 3-5 seconds, followed by a delay before reading. If you do not turn on the Tungsten lamp, the Xenon flash lamp will be used. The Xenon lamp does not need to warm up before use. No more than 350 µL should ever be added to a microplate for use in this instrument, due to the risk of spills.
2012_fall_sop_book_9th_ed_1.docx