Remarks - Method A stock solution was prepared by bubbling the test substance (gas) through dilution water for 3 hours at the rate of 20 mL/minute. Concentration mg/L Number of D. magna Number Immobilised Geometric 24 h 48 h mean Control 20 0 0 83 20 0 0 EC50 > 83 mg/L at 48 hours NOEC 83 mg/L at 48 hours Remarks - Results All validity criteria for the test were satisfied. Chemical analysis showed an initial concentration of 102 mg/L. At the end of the test period the concentration was 68 mg/L. Hence the geometrical mean value was calculated to be 83 mg/L. the test condition, the EC50 for Daphnia exposed to test substance was > 83 mg/L. Since no effect was recorded at this level, it can be concluded that 48 h EC50 for Daphnia is > 100 mg/L. The notified chemical is not harmful to aquatic invertebrates. NOTOX B.V (2006b) Algal growth inhibition test Notified chemical OECD TG 201 Alga, Growth Inhibition Test. Species Selenastrum capricornutum Exposure Period 72 hours Concentration Range Nominal: mg/L … Actual: mg/L … Auxiliary Solvent None Water Hardness 24 mg CaCO3/L Analytical Monitoring Gas chromatography Biomass Growth EbC50 NOEbC ErC50 NOErC mg/L at 72 h mg/L at 72 h mg/L at 72h mg/L at 72 h > 75 75 > 75 75 Remarks - Results on growth rate over the test period, the lowest observed effect concentration (LOEC) was determined to be 75 mg/L. The ErC50 is, therefore, concluded to be > 75 mg/L. Since no effect was recorded at > 75 mg/L and at a maximum average exposure of 114 mg/L in one of the treated solutions, it can be concluded that that the EC50 for algal growth is > 100 mg/L. The results were calculated and reported based on the mean measured concentrations. All validity criteria for the test were satisfied. The notified chemical is not harmful to algae.
Remarks - Method A stock solution was prepared by bubbling the test substance (gas) through dilution water for 3 hours at the rate of 20 mL/minute. Concentration mg/L Number of D. magna Number Immobilised Geometric mean 24 h 48 h Control 83 20 20 0 0 0 0 EC50 > 83 mg/L at 48 hours NOEC 83 mg/L at 48 hours Remarks - Results All validity criteria for the test were satisfied. Chemical analysis showed an initial concentration of 102 mg/L. At the end of the test period the concentration was 68 mg/L. Hence the geometrical mean value was calculated to be 83 mg/L. the test condition, the EC50 for Daphnia exposed to test substance was > 83 mg/L. Since no effect was recorded at this level, it can be concluded that 48 h EC50 for Daphnia is > 100 mg/L. The notified chemical is not harmful to aquatic invertebrates. NOTOX B.V (2006b) Algal growth inhibition test Notified chemical OECD TG 201 Alga, Growth Inhibition Test. Species Selenastrum capricornutum Exposure Period 72 hours Concentration Range Nominal: … mg/L Actual: … mg/L Auxiliary Solvent None Water Hardness 24 mg CaCO3/L Analytical Monitoring Gas chromatography Biomass Growth EbC50 NOEbC ErC50 NOErC mg/L at 72 h mg/L at 72 h mg/L at 72h mg/L at 72 h > 75 75 > 75 75 Remarks - Results on growth rate over the test period, the lowest observed effect concentration (LOEC) was determined to be 75 mg/L. The ErC50 is, therefore, concluded to be > 75 mg/L. Since no effect was recorded at > 75 mg/L and at a maximum average exposure of 114 mg/L in one of the treated solutions, it can be concluded that that the EC50 for algal growth is > 100 mg/L. The results were calculated and reported based on the mean measured concentrations. All validity criteria for the test were satisfied. The notified chemical is not harmful to algae.
STD1535-FR-FINAL.docx