5 < 5 5 67 10 < 5 10 75 15 < 5 15 78 20 < 5 20 79 28 < 5 28 83 Remarks - Results All validity criteria for the test were satisfied. The results obtained from the toxicity control showed that the test substance did not inhibit the biodegradation of sodium benzoate, as the total oxygen consumption in the toxicity control bottles was similar to the total of oxygen consumptions by the test substance. The test substance indicated negligible biodegradation (< 5%), and therefore it is classified as not readily biodegradable. The notified chemical is not readily biodegradable. AstraZeneca (2008) Ecotoxicological Investigations toxicity to fish Notified chemical OECD TG 203 Fish, Acute Toxicity Test - Static Species Carp (Cyprinus carpio) Exposure Period 96 hours Auxiliary Solvent None Hardness Not given Analytical Monitoring Gas chromatography Concentration mg/L Number of Fish Mortality Mean measured 24 h 48 h 72 h 96 h Control 7 0 0 0 0 197 7 0 0 0 0 LC50 > 197 mg/L NOEC 197 mg/L at 96 hours. Remarks Results All validity criteria for the test were satisfied. – The results were calculated and reported based on the mean measured concentrations. Under the test conditions, the notified chemical had no lethal effect on carp at the average measured concentration of 197 mg/L. The notified chemical is not harmful to fish. B.V (2006a) Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test - Static. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 250 mg CaCO3/L Analytical Monitoring Gas chromatography
5 10 15 20 28 < 5 < 5 < 5 < 5 < 5 5 10 15 20 28 67 75 78 79 83 Remarks - Results All validity criteria for the test were satisfied. The results obtained from the toxicity control showed that the test substance did not inhibit the biodegradation of sodium benzoate, as the total oxygen consumption in the toxicity control bottles was similar to the total of oxygen consumptions by the test substance. The test substance indicated negligible biodegradation (< 5%), and therefore it is classified as not readily biodegradable. The notified chemical is not readily biodegradable. AstraZeneca (2008) Ecotoxicological Investigations toxicity to fish Notified chemical OECD TG 203 Fish, Acute Toxicity Test - Static Species Carp (Cyprinus carpio) Exposure Period 96 hours Auxiliary Solvent None Hardness Not given Analytical Monitoring Gas chromatography Concentration mg/L Number of Fish Mortality Mean measured 24 h 48 h 72 h 96 h Control 197 7 7 0 0 0 0 0 0 0 0 LC50 > 197 mg/L NOEC 197 mg/L at 96 hours. Remarks – Results All validity criteria for the test were satisfied. The results were calculated and reported based on the mean measured concentrations. Under the test conditions, the notified chemical had no lethal effect on carp at the average measured concentration of 197 mg/L. The notified chemical is not harmful to fish. B.V (2006a) Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test - Static. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 250 mg CaCO3/L Analytical Monitoring Gas chromatography
STD1535-FR-FINAL.docx