1,3-Dimethyl-4-aminouracil (CD 19-0456) OECD TG 202 Daphnia sp. Immobilisation Test and Reproduction Test static test. – EC Directive 92/69/EEC C.2 Acute Toxicity for Daphnia - static test. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 250 mg CaCO3/L Analytical Monitoring HPLC analysis with UV/VIS-detection Remarks - Method A preliminary test was done to ascertain the concentration of the test material to be used. The study was conducted in duplicate with covered glass beakers containing 50 mL of test medium. Each beaker contained 10 daphnia (i.e. 20 daphnia per concentration), which were not fed during the study. The temperature, dissolved oxygen and pH were measured at the beginning and end of the study. Concentration mg/L Number of D. magna Number Immobilised Nominal Actual 24 h 48 h 0 (control) - 20 0 0 100 - 20 0 0 LC50 >100 mg/L at 48 NOEC (or LOEC) 100 mg/L at 48 hours Remarks - Results The test medium was a clear solution throughout the study. No abnormal behaviour or dead daphnia were observed during the study. The LC50 result indicates that under the Globally Harmonised System of Classification and Labelling of Chemicals the test substance is not classified as harmful to aquatic life. RCC Ltd (2001b) 8.2.3. Algal growth inhibition test 1,3-Dimethyl-4-aminouracil (CD 19-0456) OECD TG 201 Alga, Growth Inhibition Test. EC Directive 92/69/EEC C.3 Algal Inhibition Test. Species Algae (Scenedesmus subspicatus) Exposure Period 72 Concentration Range 100 mg/L Nominal Concentration Range 103-104 mg/L Actual Auxiliary Solvent None Water Hardness 24 mg CaCO3/L Analytical Monitoring HPLC analysis with UV/VIS-detection Remarks - Method The study consisted of 3 replicates per test concentration and 6 of the control. Erlenmeyer flask containing the solutions were inoculated with algal suspension to give a cell density of 10000 cell per mL of test medium. The flasks were covered, incubated in a temperature controlled water bath at 22-23oC and continuously illuminated. 72 hour EC50 > 100 mg/L
1,3-Dimethyl-4-aminouracil (CD 19-0456) OECD TG 202 Daphnia sp. Immobilisation Test and Reproduction Test – static test. EC Directive 92/69/EEC C.2 Acute Toxicity for Daphnia - static test. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 250 mg CaCO3/L Analytical Monitoring HPLC analysis with UV/VIS-detection Remarks - Method A preliminary test was done to ascertain the concentration of the test material to be used. The study was conducted in duplicate with covered glass beakers containing 50 mL of test medium. Each beaker contained 10 daphnia (i.e. 20 daphnia per concentration), which were not fed during the study. The temperature, dissolved oxygen and pH were measured at the beginning and end of the study. Concentration mg/L Number of D. magna Number Immobilised Nominal Actual 24 h 48 h 0 (control) - 20 0 0 100 - 20 0 0 LC50 >100 mg/L at 48 NOEC (or LOEC) 100 mg/L at 48 hours Remarks - Results The test medium was a clear solution throughout the study. No abnormal behaviour or dead daphnia were observed during the study. The LC50 result indicates that under the Globally Harmonised System of Classification and Labelling of Chemicals the test substance is not classified as harmful to aquatic life. RCC Ltd (2001b) 8.2.3. Algal growth inhibition test 1,3-Dimethyl-4-aminouracil (CD 19-0456) OECD TG 201 Alga, Growth Inhibition Test. EC Directive 92/69/EEC C.3 Algal Inhibition Test. Species Algae (Scenedesmus subspicatus) Exposure Period 72 Concentration Range Nominal 100 mg/L Concentration Range Actual 103-104 mg/L Auxiliary Solvent None Water Hardness 24 mg CaCO3/L Analytical Monitoring HPLC analysis with UV/VIS-detection Remarks - Method The study consisted of 3 replicates per test concentration and 6 of the control. Erlenmeyer flask containing the solutions were inoculated with algal suspension to give a cell density of 10000 cell per mL of test medium. The flasks were covered, incubated in a temperature controlled water bath at 22-23oC and continuously illuminated. 72 hour EC50 > 100 mg/L
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