Appendix B: Toxicological Investigations toxicity oral – Notified chemical OECD TG 423 Acute Oral Toxicity Acute Toxic Class Method. – Species/Strain Rat/Wistar Crl: WI(Han) Vehicle Cotton seed oil Remarks - Method No significant protocol deviations Group Number and Sex Dose Mortality of Animals mg/kg bw 1 3F 2,000 0/3 2 3F 2,000 0/3 LD50 > 2,000 mg/kg bw Signs of Toxicity There were no test substance-related signs of toxicity. Effects in Organs No macroscopic findings were noted at necropsy. Remarks - Results The body weight gain of the animals was within the range commonly recorded for this strain and age. The notified chemical is of low toxicity via the oral BSL (2014a) Acute toxicity dermal – Analogue 1 (morpholine, 4-[(triethoxysilyl)methyl]-, CAS No. OECD TG 402 Acute Dermal Toxicity Limit Test. – Species/Strain Rat/HsdRccHan: WIST Vehicle None Type of dressing Occlusive Remarks - Method No significant protocol deviations Group Number and Sex Dose Mortality of Animals mg/kg bw 1 5 per sex 2,000 0/10 LD50 > 2000 mg/kg bw Signs of Toxicity - Local There were no clinical signs of local toxicity. Signs of Toxicity - Systemic There were no clinical signs of local toxicity. Effects in Organs No abnormalities were noted at necropsy. Remarks - Results The body weight gain of the animals was within the range commonly recorded for this strain and age. The analogue chemical is of low toxicity via the dermal BSL (2007) Irritation skin (in –
Appendix B: Toxicological Investigations toxicity – oral Notified chemical OECD TG 423 Acute Oral Toxicity – Acute Toxic Class Method. Species/Strain Rat/Wistar Crl: WI(Han) Vehicle Cotton seed oil Remarks - Method No significant protocol deviations Group Number and Sex of Animals Dose mg/kg bw Mortality 1 3F 2,000 0/3 2 3F 2,000 0/3 LD50 > 2,000 mg/kg bw Signs of Toxicity There were no test substance-related signs of toxicity. Effects in Organs No macroscopic findings were noted at necropsy. Remarks - Results The body weight gain of the animals was within the range commonly recorded for this strain and age. The notified chemical is of low toxicity via the oral BSL (2014a) Acute toxicity – dermal Analogue 1 (morpholine, 4-[(triethoxysilyl)methyl]-, CAS No. OECD TG 402 Acute Dermal Toxicity – Limit Test. Species/Strain Rat/HsdRccHan: WIST Vehicle None Type of dressing Occlusive Remarks - Method No significant protocol deviations Group Number and Sex of Animals Dose mg/kg bw Mortality 1 5 per sex 2,000 0/10 LD50 > 2000 mg/kg bw Signs of Toxicity - Local There were no clinical signs of local toxicity. Signs of Toxicity - Systemic There were no clinical signs of local toxicity. Effects in Organs No abnormalities were noted at necropsy. Remarks - Results The body weight gain of the animals was within the range commonly recorded for this strain and age. The analogue chemical is of low toxicity via the dermal BSL (2007) Irritation – skin (in
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