respiration, and depressed or placement, righting, and pain reflexes. Animals at the three higher dosage levels also showed bloated abdomens. Death was immediately preceded by coma and a profuse bloody discharge from the eyes. Animals at the lower two dosage levels exhibited normal appearance and behaviour at 24 hours after dosage and thereafter. At 24 hours the remaining survivors appeared depressed and showed a bloody discharge around the eyes and laboured respiration, while those at 4.64 and 6.81 mL/kg levels also showed bloated abdomens, tachycardia, and depressed or absent placement and righting reflexes. These animals gradually recovered within an additional one to three days after which they appeared normal. Effects in Organs Gross autopsies performed upon the animals that died showed hyperaemic and inflated lungs, slight irritation of the small intestine and congested kidneys and adrenals. In addition the blood appeared to have a thin consistency and did not clot readily. No gross pathological findings were observed at autopsy of the surviving animals. Remarks - Results The notified chemical is of low toxicity via the oral route. Hazleton Laboratories (1957) 7.2. Acute toxicity - intravenous Notified chemical. Species/Strain Rat-Sprague Dawley Mouse- Swiss Webster Vehicle 0.9% sodium chloride solution Remarks - Method 14 day period Group Conc. Dose Number and Sex LD50 (% v/v) (mg/kg) of Animals (both sexes) Rat I 20 3160 10M/10F 5836 3980 10M/10F 5010 10M/10F 6310 10M/10F 7940 10M/10F Rat II 40 3160 10M/10F 5369 3980 10M/10F 5010 10M/10F 6310 10M/10F 7940 10M/10F Mouse I 20 4470 10M/10F 6895 5620 10M/10F 7080 10M/10F 8910 10M/10F 10M/10F Mouse II 40 2820 10M/10F 5416 3550 10M/10F 4470 10M/10F 5620 10M/10F 7080 10M/10F
respiration, and depressed or placement, righting, and pain reflexes. Animals at the three higher dosage levels also showed bloated abdomens. Death was immediately preceded by coma and a profuse bloody discharge from the eyes. Animals at the lower two dosage levels exhibited normal appearance and behaviour at 24 hours after dosage and thereafter. At 24 hours the remaining survivors appeared depressed and showed a bloody discharge around the eyes and laboured respiration, while those at 4.64 and 6.81 mL/kg levels also showed bloated abdomens, tachycardia, and depressed or absent placement and righting reflexes. These animals gradually recovered within an additional one to three days after which they appeared normal. Effects in Organs Gross autopsies performed upon the animals that died showed hyperaemic and inflated lungs, slight irritation of the small intestine and congested kidneys and adrenals. In addition the blood appeared to have a thin consistency and did not clot readily. No gross pathological findings were observed at autopsy of the surviving animals. Remarks - Results The notified chemical is of low toxicity via the oral route. Hazleton Laboratories (1957) 7.2. Acute toxicity - intravenous Notified chemical. Species/Strain Rat-Sprague Dawley Mouse- Swiss Webster Vehicle 0.9% sodium chloride solution Remarks - Method 14 day period Group Conc. (% v/v) Dose (mg/kg) Number and Sex of Animals LD50 (both sexes) Rat I 20 3160 3980 5010 6310 7940 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 5836 Rat II 40 3160 3980 5010 6310 7940 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 5369 Mouse I 20 4470 5620 7080 8910 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 6895 Mouse II 40 2820 3550 4470 5620 7080 10M/10F 10M/10F 10M/10F 10M/10F 10M/10F 5416
STD-1052-FR.docx