indicated that the test substance was algistatic in effect. All the test guideline validity criteria were met. The notified chemicals are harmful to freshwater alga Harlan (2013d) Algal growth inhibition test The inseparable mixture of the notified chemicals ISO 1995 Quality Marine Algal Growth Inhibition Test With – and ISO 10253 Skeletonema costatum Phaeodactylum tricornutum, standard method Species Skeletonema costatum Exposure Period 72 hours Concentration Range Nominal: Control, 10, 50, 250 and 1000 mg/L Actual: Not determined Auxiliary Solvent None Water Hardness Not provided Analytical Monitoring N/A Remarks - Method Following a preliminary test, the definitive test was conducted by exposing algae in the test medium at the above nominal concentrations. GLP standards were followed. Growth Biomass ErC50 NOEC NOEC mg/L at 72 h mg/L mg/L at 72 h mg/L 672 250 N/A N/A Remarks - Results All the test guideline validity criteria were met. The notified chemicals are not harmful to marine algae AnalyCen (2009) Inhibition of microbial activity The inseparable mixture of the notified chemicals OECD TG 209 Activated Sludge, Respiration Inhibition Test. EC Directive 88/302/EEC C.11 Biodegradation: Activated Sludge Respiration Inhibition Test. Inoculum Activated sewage sludge Exposure Period 3 Concentration Range Nominal: 10, 100, 1000 mg/L Actual: Not determined Remarks Method – The test was conducted at 10 1000 mg/L. Three replicates were – established for the top test concentration of 1000 mg/L. 3,5-Dichlorophenol was used for the reference test. The test was conducted according to the test guidelines above with no significant deviation from the protocol. GLP standards were followed. IC50 > 1000 mg/L NOEC 1000 mg/L Remarks Results – All the test guideline validity criteria were met.
indicated that the test substance was algistatic in effect. All the test guideline validity criteria were met. The notified chemicals are harmful to freshwater alga Harlan (2013d) Algal growth inhibition test The inseparable mixture of the notified chemicals ISO 1995 – Quality Marine Algal Growth Inhibition Test With Skeletonema costatum and Phaeodactylum tricornutum, ISO 10253 standard method Species Skeletonema costatum Exposure Period 72 hours Concentration Range Nominal: Control, 10, 50, 250 and 1000 mg/L Actual: Not determined Auxiliary Solvent None Water Hardness Not provided Analytical Monitoring N/A Remarks - Method Following a preliminary test, the definitive test was conducted by exposing algae in the test medium at the above nominal concentrations. GLP standards were followed. Growth Biomass ErC50 NOEC NOEC mg/L at 72 h mg/L mg/L at 72 h mg/L 672 250 N/A N/A Remarks - Results All the test guideline validity criteria were met. The notified chemicals are not harmful to marine algae AnalyCen (2009) Inhibition of microbial activity The inseparable mixture of the notified chemicals OECD TG 209 Activated Sludge, Respiration Inhibition Test. EC Directive 88/302/EEC C.11 Biodegradation: Activated Sludge Respiration Inhibition Test. Inoculum Activated sewage sludge Exposure Period 3 Concentration Range Nominal: 10, 100, 1000 mg/L Actual: Not determined Remarks – Method The test was conducted at 10 – 1000 mg/L. Three replicates were established for the top test concentration of 1000 mg/L. 3,5-Dichlorophenol was used for the reference test. The test was conducted according to the test guidelines above with no significant deviation from the protocol. GLP standards were followed. IC50 > 1000 mg/L NOEC 1000 mg/L Remarks – Results All the test guideline validity criteria were met.
STD-1474-FR.docx