Harlan (2015a) Algal growth inhibition test Notified chemical OECD TG 201 Alga, Growth Inhibition Test. Species Exposure Period 72 Concentration Nominal: Control, solvent control, 0.13, 0.25, 0.5, 1.0, 2.0 and 4.0 mg/L Actual: Not determined Auxiliary Solvent Tetrahydrofuran Water Hardness 15 mg Analytical Monitoring No Remarks - Method The test substance hydrolysed rapidly in the test solutions. A measurement of total organic carbon was not reasonable due to the low test item concentrations and the use of the solvent tetrahydrofuran. Therefore, the analysis of the test concentration in the test solutions was not conducted. Growth EC50 NOEC mg/L at 72 h mg/L at 72 h > 4.0 > 4.0 Remarks - Results The validity criteria for the test are satisfied. Based on visual inspection of the test media, the solubility limit to achieve stable solutions of the test substance or its hydrolysis product was considered to be below 4.0 mg/L. No acute toxic effects was observed up to this maximum technically feasible concentration, 4.0 mg/L, of the test substance or its hydrolysis products in test water under the test conditions. Hydrolysis products of the notified chemical are not expected to be toxic to aquatic invertebrates up to the maximum technically feasible concentration Harlan
Harlan (2015a) Algal growth inhibition test Notified chemical OECD TG 201 Alga, Growth Inhibition Test. Species Exposure Period 72 Concentration Nominal: Control, solvent control, 0.13, 0.25, 0.5, 1.0, 2.0 and 4.0 mg/L Actual: Not determined Auxiliary Solvent Tetrahydrofuran Water Hardness 15 mg Analytical Monitoring No Remarks - Method The test substance hydrolysed rapidly in the test solutions. A measurement of total organic carbon was not reasonable due to the low test item concentrations and the use of the solvent tetrahydrofuran. Therefore, the analysis of the test concentration in the test solutions was not conducted. Growth EC50 NOEC mg/L at 72 h mg/L at 72 h > 4.0 > 4.0 Remarks - Results The validity criteria for the test are satisfied. Based on visual inspection of the test media, the solubility limit to achieve stable solutions of the test substance or its hydrolysis product was considered to be below 4.0 mg/L. No acute toxic effects was observed up to this maximum technically feasible concentration, 4.0 mg/L, of the test substance or its hydrolysis products in test water under the test conditions. Hydrolysis products of the notified chemical are not expected to be toxic to aquatic invertebrates up to the maximum technically feasible concentration Harlan
STD1615FR.DOCX