Test substance Sodium Benzoate Day % Degradation Day % Degradation 1 - 1 - 3 0 3 65 7 0 7 78 10 0 10 86 14 0 14 91 21 0 21 92 28 0 28 93 Remarks Results The toxicity control attained 68% degradation after 14 days confirming – that the test substance was not inhibitory to activated sludge bacteria. The reference substance showed the expected degradation profile, confirming the suitability of the inoculums and the validity of the test conditions. The deviations to protocol are not expected to significantly alter the outcome of the test. The notified chemical is not inherently biodegradable RCC Ltd (2008o) Ecotoxicological Investigations Acute toxicity to fish Notified chemical OECD TG 203 Fish, Acute Toxicity Test Semi- static – EC Directive 92/69/EEC C.1 Acute Toxicity for Fish Semi- static – Species Zebra fish (Brachydanio rerio) Exposure Period 96 hours Auxiliary Solvent None Water Hardness 125 mg CaCO3/L Analytical Monitoring HPLC Remarks Method Following a range-finding test a limit test was conducted in accordance – with the guidelines above and with GLP compliance. No deviations to protocol were reported. Concentration mg/L Number of Fish Mortality Nominal Actual 3 h 24 h 48 h 72 h 96 h Control 0 7 0 0 0 0 0 100 98-104 7 0 0 0 0 0 LC50 > 100 mg/L at 96 hours (based on nominal concentration) NOEC > 100 mg/L at 96 hours (based on nominal concentration) Remarks Results The analytically determined mean test item concentration in the test – medium at the start and the end of the test medium renewal periods of 24 hours was 98 and 104% of the nominal value, respectively. Under the test conditions, the test substance was stable during the test period of 96 h. Therefore, all reported results are related to the nominal concentration. All validity criteria for the test were satisfied. The notified chemical is not harmful to fish Harlan Laboratories (2009h)
Test substance Sodium Benzoate Day % Degradation Day % Degradation 1 - 1 - 3 0 3 65 7 0 7 78 10 0 10 86 14 0 14 91 21 0 21 92 28 0 28 93 Remarks – Results The toxicity control attained 68% degradation after 14 days confirming that the test substance was not inhibitory to activated sludge bacteria. The reference substance showed the expected degradation profile, confirming the suitability of the inoculums and the validity of the test conditions. The deviations to protocol are not expected to significantly alter the outcome of the test. The notified chemical is not inherently biodegradable RCC Ltd (2008o) Ecotoxicological Investigations Acute toxicity to fish Notified chemical OECD TG 203 Fish, Acute Toxicity Test – Semi- static EC Directive 92/69/EEC C.1 Acute Toxicity for Fish – Semi- static Species Zebra fish (Brachydanio rerio) Exposure Period 96 hours Auxiliary Solvent None Water Hardness 125 mg CaCO3/L Analytical Monitoring HPLC Remarks – Method Following a range-finding test a limit test was conducted in accordance with the guidelines above and with GLP compliance. No deviations to protocol were reported. Concentration mg/L Number of Fish Mortality Nominal Actual 3 h 24 h 48 h 72 h 96 h Control 0 7 0 0 0 0 0 100 98-104 7 0 0 0 0 0 LC50 > 100 mg/L at 96 hours (based on nominal concentration) NOEC > 100 mg/L at 96 hours (based on nominal concentration) Remarks – Results The analytically determined mean test item concentration in the test medium at the start and the end of the test medium renewal periods of 24 hours was 98 and 104% of the nominal value, respectively. Under the test conditions, the test substance was stable during the test period of 96 h. Therefore, all reported results are related to the nominal concentration. All validity criteria for the test were satisfied. The notified chemical is not harmful to fish Harlan Laboratories (2009h)
STD1385FR.docx