4. Sequencing Requested (Mandatory, except where stated): Species: Reference genome & release version. Only required if requesting genomic alignment of DNA reads. Not available for RNA: Sequencing type: Single Read Paired End Desired insert size (default 350 bp). Only required if submitting DNA samples for library prep: Sequencing Instrument requested: 2500, high output mode (leave blank if unsure) HiSeq 2500, rapid mode HiSeq 3/4000 HiSeq X NextSeq 500 (mid output reagents) NextSeq 500 (high output reagents) MiSeq Read Length: 40 bp (N high output paired end only) – 50 bp (M, H2, H2-rapid, H3/4) (Reagents stocked as standard in bold others must – be ordered, and in the case of HiSeq can only be 75 bp (N high output single read only) – ordered as full flowcells). Note that HiSeq X can only 75 bp (N, H3/4 paired end only) – be selected for whole-genome sequencing projects. 100 bp (H2, H3/4, H2-rapid) 125 bp (H2, high output) H2 = HiSeq 2500 150 bp (H3/4, HX) H3/4 = 4000 150 bp (M, N) HX = HiSeq X 250 bp (M, H2-rapid) M = MiSeq 300 bp (M) N = NextSeq Total number lanes (HiSeq) or runs (MiSeq / NextSeq) requested: Project Goal (brief description): REK approval number if sequencing human samples: 5. Select Data delivery format (Select one - mandatory):
4. Sequencing Requested (Mandatory, except where stated): Species: Reference genome & release version. Only required if requesting genomic alignment of DNA reads. Not available for RNA: Sequencing type: Single Read Paired End Desired insert size (default 350 bp). Only required if submitting DNA samples for library prep: Sequencing Instrument requested: (leave blank if unsure) 2500, high output mode HiSeq 2500, rapid mode HiSeq 3/4000 HiSeq X NextSeq 500 (mid output reagents) NextSeq 500 (high output reagents) MiSeq Read Length: (Reagents stocked as standard in bold – others must be ordered, and in the case of HiSeq can only be ordered as full flowcells). Note that HiSeq X can only be selected for whole-genome sequencing projects. H2 = HiSeq 2500 H3/4 = 4000 HX = HiSeq X M = MiSeq N = NextSeq 40 bp (N – high output paired end only) 50 bp (M, H2, H2-rapid, H3/4) 75 bp (N – high output single read only) 75 bp (N, H3/4 – paired end only) 100 bp (H2, H3/4, H2-rapid) 125 bp (H2, high output) 150 bp (H3/4, HX) 150 bp (M, N) 250 bp (M, H2-rapid) 300 bp (M) Total number lanes (HiSeq) or runs (MiSeq / NextSeq) requested: Project Goal (brief description): REK approval number if sequencing human samples: 5. Select Data delivery format (Select one - mandatory):
sample-submission-form-(illumina).docx