more than a centimeter or so of the We will also be examining urine medium. and stool specimens for pathogenic bacteria. the inoculating loop, streak the Methods and Materials plate for isolation. Repeat steps 1 through 3 using a Materials MSA plate. Blood agar plates Place the blood agar and MSA plates MSA plates into the incubator. MAC agar plates To perform the nasal swab, carefully EMB plates place a sterile swab into the nostril B-G plates and gently swab. Bile-esculin agar plates Repeat steps 2 and 3 using blood Optochin disks agar and MSA. Bacitracin disks Place the blood agar and MSA plates Sterile saline into the incubator. Sterile swabs Be sure to properly label each plate Oxidase reagent Microscope slides Urine Specimens Gram stain reagents Inoculating loop Obtain a prepared urine specimen Bunsen burner from the instructor. Hydrogen peroxide Prepare Gram stains of the Commercial identification kits prepared urine specimen. Be Sterile pipettes sure to note the typical Pipettor morphology of each organism Test tube rack and its staining characteristics. Parafilm Record your results in Table 2. Toothpicks Inoculate a sample of the Wooden tongue depressors prepared urine specimen onto the GasPak Chamber and CO2 appropriate media: MAC, Blood agar, Bile-esculin agar. The Protocol - Day One instructor will demonstrate the proper inoculation techniques. Throat and Nasal Swabs Incubate the plates at 37oC overnight. Using the tongue depressor to hold down your lab partner’s tongue, take Stool Specimens the sterile swab and gently swab the back of their throat. Be careful not Obtain a prepared stool specimen to probe too deeply, as this will elicit from the instructor. a gag response. Inoculate the enteric media: Immediately after swabbing the EMB agar MAC agar, and B-G throat, roll the swab over the edge of agar, as directed by the the blood agar plate, contacting no instructor.
We will also be examining urine and stool specimens for pathogenic bacteria. Methods and Materials Materials Blood agar plates MSA plates MAC agar plates EMB plates B-G plates Bile-esculin agar plates Optochin disks Bacitracin disks Sterile saline Sterile swabs Oxidase reagent Microscope slides Gram stain reagents Inoculating loop Bunsen burner Hydrogen peroxide Commercial identification kits Sterile pipettes Pipettor Test tube rack Parafilm Toothpicks Wooden tongue depressors GasPak Chamber and CO2 Protocol - Day One Throat and Nasal Swabs Using the tongue depressor to hold down your lab partner’s tongue, take the sterile swab and gently swab the back of their throat. Be careful not to probe too deeply, as this will elicit a gag response. Immediately after swabbing the throat, roll the swab over the edge of the blood agar plate, contacting no more than a centimeter or so of the medium. the inoculating loop, streak the plate for isolation. Repeat steps 1 through 3 using a MSA plate. Place the blood agar and MSA plates into the incubator. To perform the nasal swab, carefully place a sterile swab into the nostril and gently swab. Repeat steps 2 and 3 using blood agar and MSA. Place the blood agar and MSA plates into the incubator. Be sure to properly label each plate Urine Specimens Obtain a prepared urine specimen from the instructor. Prepare Gram stains of the prepared urine specimen. Be sure to note the typical morphology of each organism and its staining characteristics. Record your results in Table 2. Inoculate a sample of the prepared urine specimen onto the appropriate media: MAC, Blood agar, Bile-esculin agar. The instructor will demonstrate the proper inoculation techniques. Incubate the plates at 37oC overnight. Stool Specimens Obtain a prepared stool specimen from the instructor. Inoculate the enteric media: EMB agar MAC agar, and B-G agar, as directed by the instructor.
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