Appendix Tables Table 1. Histological classification of oocyte development. Reproductive State Gonad Stage Diagnostics nucleus (germinal vesicle) surrounded by a thin layer Chromatin of cytoplasm. Stains darkly and Undeveloped nucleolar uniformly with Toluidine Blue. The germinal vesicle increases in size and nucleli appear at its periphery. The cytoplasm stains uniformly with Toluidine Undeveloped Perinucleolar Blue. Appearance of cotical alveoli (yolk vesicles) in the cytoplasm and Cortical formation of the vitelline Developing alveoli membrane. Appearance of yolk proteins. Mature- The oocyte dramatically increases Spawning Capable Vitellogenic in size. Beginning of hydration (coalescence of yolk granules and migration and dissolution of the Spawning Capable Hydrated 1 nucleus). Oocyte round. Mature- Oocyte fully hydrated. Spawning Capable Hyrdated 2 Oocyte shape irregular. Presence of retained oocytes post spawning. Atresic oocytes lose Mature- their spherical appearance and the Spent membrane breaks down. Post (Regressing) Atresic ovulatory follicles (POFs) present. Oocytes in various stages. No Chromatic vitellogenic oocytes present. The nucleolar, wall is thick and the ovary may Perinucleolar, contain unabsorbed material from Cortical alveoli past spawning events.
Spawning Appendix Tables Table 1. Histological classification of oocyte development. Reproductive State Gonad Stage Diagnostics Undeveloped Chromatin nucleolar nucleus (germinal vesicle) surrounded by a thin layer of cytoplasm. Stains darkly and uniformly with Toluidine Blue. Undeveloped Perinucleolar The germinal vesicle increases in size and nucleli appear at its periphery. The cytoplasm stains uniformly with Toluidine Blue. Developing Cortical alveoli Appearance of cotical alveoli (yolk vesicles) in the cytoplasm and formation of the vitelline membrane. Mature- Spawning Capable Vitellogenic Appearance of yolk proteins. The oocyte dramatically increases in size. Capable Hydrated 1 Beginning of hydration (coalescence of yolk granules and migration and dissolution of the nucleus). Oocyte round. Mature- Spawning Capable Hyrdated 2 Oocyte fully hydrated. Oocyte shape irregular. Mature- Spent (Regressing) Atresic Presence of retained oocytes post spawning. Atresic oocytes lose their spherical appearance and the membrane breaks down. Post ovulatory follicles (POFs) present. Chromatic nucleolar, Perinucleolar, Cortical alveoli Oocytes in various stages. No vitellogenic oocytes present. The wall is thick and the ovary may contain unabsorbed material from past spawning events.
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