Standard Operating Procedure for: PPE required: Degassing liquids - sonication For many experiments it may be desirable to reduce the amount of dissolved oxygen and other dissolved gases. There are four main methods: pressure reduction, heating, sonication and displacement by an inert gas (sparging). Only sonication will be discussed here. This SOP can be used for degassing water, for any other substance an addition risk assessment is required. Cold sonication Hazard symbols: This technique is suitable for many volatile and reactive chemicals; however, you should make your own assessment of this and any Other hazards other risks from the chemicals you are using. SOME CHEMICALS from individual MAY GET HOT DURING THIS PROCESS which can result in an experiments Significant EXPLOSION. hazards: Put your liquid into a clean bottle (or another container with a Noise from sonicating bath narrow opening) with a loose fitting lid or covering. Burst blood Make sure the sonic bath is filled up to the appropriate level, vessels from contact with Put it into a sonic bath and commence sonication for desired time. sonication Other hazards Once sonication is complete IMMEDIATELY tighten the lid on the from individual bottle or seal using parafilm. experiments Hazard Heated sonication phrases: This technique is not suitable for volatile chemicals or where undesired chemical reactions or decompositions may take place. You Can it be done should make your own assessment of this and any other risks out of hours? from the chemicals you are using. Degassing by Put your liquid into a clean bottle (or another container with a sonication can narrow opening) with a loose fitting lid or covering. Beware of lids be performed from volumetric flasks popping off. out of hours so long as risk Make sure sonic bath is filled up to the appropriate level, assessment of Put it into a sonic bath and commence sonication. Set the individual experiments temperature of the bath to an appropriate temperature. Place sign allow. to warn of hot liquids. Reduce temperature back to ambient continuing sonication throughout, Once ambient temperature is reach and sonication is complete IMMEDIATELY tighten the lid on the bottle or seal using parafilm. This SOP is not relevant in the following circumstances: SOP does not cover specific experimental risk these must be covered by user’s assessments Any other situation where the procedure may result in harm to yourself or others.
Standard Operating Procedure for: Degassing liquids - sonication PPE required: For many experiments it may be desirable to reduce the amount of dissolved oxygen and other dissolved gases. There are four main methods: pressure reduction, heating, sonication and displacement by an inert gas (sparging). Only sonication will be discussed here. This SOP can be used for degassing water, for any other substance an addition risk assessment is required. Cold sonication This technique is suitable for many volatile and reactive chemicals; however, you should make your own assessment of this and any other risks from the chemicals you are using. SOME CHEMICALS MAY GET HOT DURING THIS PROCESS which can result in an EXPLOSION. Put your liquid into a clean bottle (or another container with a narrow opening) with a loose fitting lid or covering. Make sure the sonic bath is filled up to the appropriate level, Put it into a sonic bath and commence sonication for desired time. Once sonication is complete IMMEDIATELY tighten the lid on the bottle or seal using parafilm. Heated sonication This technique is not suitable for volatile chemicals or where undesired chemical reactions or decompositions may take place. You should make your own assessment of this and any other risks from the chemicals you are using. Put your liquid into a clean bottle (or another container with a narrow opening) with a loose fitting lid or covering. Beware of lids from volumetric flasks popping off. Make sure sonic bath is filled up to the appropriate level, Put it into a sonic bath and commence sonication. Set the temperature of the bath to an appropriate temperature. Place sign to warn of hot liquids. Reduce temperature back to ambient continuing sonication throughout, Once ambient temperature is reach and sonication is complete IMMEDIATELY tighten the lid on the bottle or seal using parafilm. Hazard symbols: Other hazards from individual experiments Significant hazards: Noise from sonicating bath Burst blood vessels from contact with sonication Other hazards from individual experiments Hazard phrases: Can it be done out of hours? Degassing by sonication can be performed out of hours so long as risk assessment of individual experiments allow. This SOP is not relevant in the following circumstances: SOP does not cover specific experimental risk these must be covered by user’s assessments Any other situation where the procedure may result in harm to yourself or others.
SOP_degassing_liquids_v2.docx