breeding of animals, and whether this is distinctively different and always acceptable. The possibility of animals suffering as a result of genetic modification could be considered. 4.4.8 Outline a basic 2 The use of technique used E. coli in gene for gene technology is transfer well involving documented. plasmids, a host Most of its cell (bacterium, DNA is in one yeast or other circular cell), restriction chromosome, enzymes but it also has (endonucleases) plasmids and DNA (smaller ligase. circles of DNA). These plasmids can be removed and cleaved by restriction enzymes at target sequences. DNA fragments from another organism can also be cleaved by the same restriction enzyme, and these pieces can be added to the open plasmid and spliced together by ligase. The recombinant plasmids
breeding of animals, and whether this is distinctively different and always acceptable. The possibility of animals suffering as a result of genetic modification could be considered. 4.4.8 Outline a basic technique used for gene transfer involving plasmids, a host cell (bacterium, yeast or other cell), restriction enzymes (endonucleases) and DNA ligase. 2 The use of E. coli in gene technology is well documented. Most of its DNA is in one circular chromosome, but it also has plasmids (smaller circles of DNA). These plasmids can be removed and cleaved by restriction enzymes at target sequences. DNA fragments from another organism can also be cleaved by the same restriction enzyme, and these pieces can be added to the open plasmid and spliced together by ligase. The recombinant plasmids
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