the Sor River. The bars represent the mean and SEM of the percentage of infected animals in one year. (*) significant differences regarding to results obtained in gills and gland (p≥0.05). Figure 5. A proportional representation of the Alcabre and Sor bacteria identified by 16S rDNA sequencing. The percentages indicate the representation of each group in the entire population of analysed bacteria from Alcabre and the Sor River (A). The distribution of bacteria within the different ecological compartments (water, sediment, plankton, mussel and oyster) in Alcabre (B) and the Sor River (C). Figure 6. (A) The cumulative mortality of mussels after a bath infection with V. splendidus and at 15 ºC (upper panel) and 25 ºC (lower panel). The effect of an 8 h V. aestuarianus emersion, which mimics hypoxia-induced stress, on the cumulative mortality was determined (left panels) (N=540 animals). (B) The cumulative mortality of mussels after an intramuscular injection of three different doses of and at 15 ºC (N=420 V. splendidus V. aestuarianus animals). The cumulative mortality from experimental infections by intramuscular injection Figure 7. of isolated from Alcabre (A) and the Sor River (B) and isolated V. splendidus V. aestuarianus from the Sor River (C). The results are presented as the mean and the SEM of two independent experimental infections (N=390 animals). Detection of (V. and (V. in Table 1. Vibrio splendidus spl) V. aestuarianus aest) environmental samples and macrofauna by qPCR. (+): positive detection; (-): negative detection; NA: not analysed.
the Sor River. The bars represent the mean and SEM of the percentage of infected animals in one year. (*) significant differences regarding to results obtained in gills and gland (p≥0.05). Figure 5. A proportional representation of the Alcabre and Sor bacteria identified by 16S rDNA sequencing. The percentages indicate the representation of each group in the entire population of analysed bacteria from Alcabre and the Sor River (A). The distribution of bacteria within the different ecological compartments (water, sediment, plankton, mussel and oyster) in Alcabre (B) and the Sor River (C). Figure 6. (A) The cumulative mortality of mussels after a bath infection with V. splendidus and V. aestuarianus at 15 ºC (upper panel) and 25 ºC (lower panel). The effect of an 8 h emersion, which mimics hypoxia-induced stress, on the cumulative mortality was determined (left panels) (N=540 animals). (B) The cumulative mortality of mussels after an intramuscular injection of three different doses of V. splendidus and V. aestuarianus at 15 ºC (N=420 animals). Figure 7. The cumulative mortality from experimental infections by intramuscular injection of V. splendidus isolated from Alcabre (A) and the Sor River (B) and V. aestuarianus isolated from the Sor River (C). The results are presented as the mean and the SEM of two independent experimental infections (N=390 animals). Table 1. Detection of Vibrio splendidus (V. spl) and V. aestuarianus (V. aest) in environmental samples and macrofauna by qPCR. (+): positive detection; (-): negative detection; NA: not analysed.
Romero_occurrence_seasonality.docx