4°C ∞ 5-2-4. Analysis of amplified products by gel electrophoresis The PCR product (10 was with 2 of Blue 6x loading dye and run on a 2% μl) μl agarose gel. In addition, 6 of 1 Kb Hyper ladder loading in the gel to confirm the μl correct sized of product. The PCR test sample (10µl) positive and negative controls were then placed in 2% agarose gel wells and (2µl) of 6× loading dye was placed in wells. The gel was immersed in TAE buffer 1× with 2.5µl ethidium bromide. 6 µl of Hyper Ladder 200 bp (Bioline, UK) was used in this experiment. Finally, the samples were subjected to electrophoresis for 40 minutes at 80V. The amplified products were finally visualized on the gel and digital images were taken using attached to a digital “UVitec” camera. Results and Discussion 5-3. 5.3. The occurrence or absence of in different environmental samples. Mycoplasma Samples Results Phosphate spoil Negative Desert soil Negative Desert non-vegetated soil Negative
∞ 4°C 5-2-4. Analysis of amplified products by gel electrophoresis The PCR product (10 μl) was with 2 μl of Blue 6x loading dye and run on a 2% agarose gel. In addition, 6 μl of 1 Kb Hyper ladder loading in the gel to confirm the correct sized of product. The PCR test sample (10µl) positive and negative controls were then placed in 2% agarose gel wells and (2µl) of 6× loading dye was placed in wells. The gel was immersed in TAE buffer 1× with 2.5µl ethidium bromide. 6 µl of Hyper Ladder 200 bp (Bioline, UK) was used in this experiment. Finally, the samples were subjected to electrophoresis for 40 minutes at 80V. The amplified products were finally visualized on the gel and digital images were taken using “UVitec” attached to a digital camera. 5-3. Results and Discussion 5.3. The occurrence or absence of Mycoplasma in different environmental samples. Samples Results Phosphate spoil Negative Desert soil Negative Desert non-vegetated soil Negative
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