Please fill out the following table Source of Insert Name of Name of Host(s) Nature of If an attempt will DNA/RNA Vector(s) Used Used (bacteria or Inserted DNA be made to obtain virus or cells) (Species) List them for all Sequences expression of a in the List them for all foreign gene, cloning process steps in the indicate the cloning process protein that will be produced Answer the following questions, use additional if needed specify Additional info needed Yes No a) Does the inserted gene encode a known oncogene? Yes No b) Does the viral DNA integrate into the host genome? Yes c) Does the modification have the potential to increase the replication capacity of virus? See section I10: No Does the modification increase the pathogenicity of the Evaluation of agent? Dual use Does the modification change the host range of the agent? Yes e) Does the inserted gene have the potential for altering the No cell cycle? Yes h) Is there a probability of generating replication-competent No viruses?
Please fill out the following table Source of Insert DNA/RNA (Species) Name of Vector(s) Used List them for all in the cloning process Name of Host(s) Used (bacteria or virus or cells) List them for all steps in the cloning process Nature of Inserted DNA Sequences If an attempt will be made to obtain expression of a foreign gene, indicate the protein that will be produced Answer the following questions, use additional if needed Additional info needed specify a) Does the inserted gene encode a known oncogene? Yes No b) Does the viral DNA integrate into the host genome? Yes No c) Does the modification have the potential to increase the replication capacity of virus? Does the modification increase the pathogenicity of the agent? Does the modification change the host range of the agent? Yes No See section I10: Evaluation of Dual use e) Does the inserted gene have the potential for altering the cell cycle? Yes No h) Is there a probability of generating replication-competent viruses? Yes No
SA_BSL3_4_NOU_3_12a.docx