known quantities, is determined by the duration of recirculation, is measured by the n experiment, leaving only to be determined experimentally. It seems plausible that is f f approximately constant, but only for small values of n. Cost Considerations The cost of the CD4 counter per test is given by the detailed breakdown presented in Table 2. Table 2: Cost analysis of the point-of-care diagnostic device Item Amount Capital Cost Cost per test PDMS base and curing agent 3g $0.31 $0.31 Sylgard 184 Anti-CD4 antibodies .3ug $ 0.14 $0.14 Europium nanoparticles 10uL $1.07 $1.07 Glass Slides 1 $0.12 $0.12 PEEK tubing 20cm $0.40 $0.04 Photolithography (500k tests) 1 $10 $0.01 Pump (500k tests) 1 $60 $0.01 TRF Reader (500k tests) 1 $1020 $0.01 Total -- $1,092 $1.71 Note that the PDMS base and curing agent are used in the making of the trap device as well as the pump. The costs of photolithography, the pump (motor and controls), and the TRF reader become negligible when their capital costs are divided by the number of tests they are expected to last: we estimate this to be about 500,000 tests. The price of the TRF reader estimated here is for a production model, rather than the prototype model used in this project. Conclusions, Recommendations, and Future Directions Significant progress toward a functional prototype for a point-of-care HIV diagnostic system has been accomplished. All of the components of the device were designed, fabricated, and tested for
known quantities, n is determined by the duration of recirculation, is measured by the experiment, leaving only f to be determined experimentally. It seems plausible that f is approximately constant, but only for small values of n. Cost Considerations The cost of the CD4 counter per test is given by the detailed breakdown presented in Table 2. Table 2: Cost analysis of the point-of-care diagnostic device Item Amount Capital Cost Cost per test PDMS base and curing agent Sylgard 184 3g $0.31 $0.31 Anti-CD4 antibodies .3ug $ 0.14 $0.14 Europium nanoparticles 10uL $1.07 $1.07 Glass Slides 1 $0.12 $0.12 PEEK tubing 20cm $0.40 $0.04 Photolithography (500k tests) 1 $10 $0.01 Pump (500k tests) 1 $60 $0.01 TRF Reader (500k tests) 1 $1020 $0.01 Total -- $1,092 $1.71 Note that the PDMS base and curing agent are used in the making of the trap device as well as the pump. The costs of photolithography, the pump (motor and controls), and the TRF reader become negligible when their capital costs are divided by the number of tests they are expected to last: we estimate this to be about 500,000 tests. The price of the TRF reader estimated here is for a production model, rather than the prototype model used in this project. Conclusions, Recommendations, and Future Directions Significant progress toward a functional prototype for a point-of-care HIV diagnostic system has been accomplished. All of the components of the device were designed, fabricated, and tested for
Senior_Design_Paper_--_Point-of-care_CD4_counter_for_resource-poor_areas.docx