five: 64.90±0.15) while there was no significant difference between the sites two (46.84±1.22%) and three (47.83±0.25%). CpG site methylation was generally uncorrelated among different CpG sites (8 / 10 correlation coefficients were below 0.30) and the strength of correlation declined with increasing physical distance between dyads of CpG sites (Supplementary Table S3). Age and heterozygosity dependent patterns of DNA methylation We next investigated the effects of age and on the percentage of DNA methylation at sMLH five different CpG sites within the gene (Table 1, Figure 3). When all of the sites were AgRP analysed together, we found significant interaction between age and (linear) (Table 1). sMLH This pattern was also evident at the level of individual CpG sites. Neither age nor were sMLH related to DNA methylation at CpG site one, but a significant interaction between and sMLH age was found at CpG site two. More specifically, DNA methylation at this CpG site was declined more strongly with increasing when individuals’ age increased (ca. four years sMLH of age or above). For CpG site three, there was a significant effect of age and the age x sMLH interaction was close to significance (contrary to CpG site two, this tendency suggests that DNA methylation increased with in older individuals; Figure 3b). By contrast, CpG sMLH sites four and five showed highly consistent patterns with both models retaining significant interactions between and both the linear terms, and either significant (Site 5) or near sMLH significant (Site 4) interactions with the quadratic age terms (Table 1). In both cases, there was a positive relationship between methylation and at intermediate ages (3–4 years of age, sMLH Figs 3c and d). DNA methylation and UV blue chroma
five: 64.90±0.15) while there was no significant difference between the sites two (46.84±1.22%) and three (47.83±0.25%). CpG site methylation was generally uncorrelated among different CpG sites (8 / 10 correlation coefficients were below 0.30) and the strength of correlation declined with increasing physical distance between dyads of CpG sites (Supplementary Table S3). Age and heterozygosity dependent patterns of DNA methylation We next investigated the effects of age and sMLH on the percentage of DNA methylation at five different CpG sites within the AgRP gene (Table 1, Figure 3). When all of the sites were analysed together, we found significant interaction between age and sMLH (linear) (Table 1). This pattern was also evident at the level of individual CpG sites. Neither age nor sMLH were related to DNA methylation at CpG site one, but a significant interaction between sMLH and age was found at CpG site two. More specifically, DNA methylation at this CpG site was declined more strongly with increasing sMLH when individuals’ age increased (ca. four years of age or above). For CpG site three, there was a significant effect of age and the age x sMLH interaction was close to significance (contrary to CpG site two, this tendency suggests that DNA methylation increased with sMLH in older individuals; Figure 3b). By contrast, CpG sites four and five showed highly consistent patterns with both models retaining significant interactions between sMLH and both the linear terms, and either significant (Site 5) or near significant (Site 4) interactions with the quadratic age terms (Table 1). In both cases, there was a positive relationship between methylation and sMLH at intermediate ages (3–4 years of age, Figs 3c and d). DNA methylation and UV blue chroma
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