Methanol (reagent 275.0 mL 200.0 mL Reagent 3 Hexane (HPLC grade) 200.0 mL Methyl-tert butyl ether (HPLC grade) 10.8 g 900.0 mL Reagent 4 Sodium hydroxide (certified) Deionized distilled water Safranin dye solution Safranin 1.0 g Distilled water 100.0 mL Schweppe’s reagent Solution 1 Glucose 2.0 g Distilled 20.0 mL Solution 2 Aniline 2.0 g Ethanol (95%) 20.0 mL Mix solutions 1 and 2 and dilute to 60 mL with alcohol. Always use freshly prepared reagent (can be stored for max. 24 hours at 4-8°C). TRIS [tris(hydroxymethyl)aminomethane] buffer (1M, pH 8.0) Trizma base (Tris base) 121.1 g Double distilled water (fill up to) 1000.0 mL HCl (cc.) 42.2 mL pH: 8.0
Methanol (reagent Reagent 3 Hexane (HPLC grade) Methyl-tert butyl ether (HPLC grade) Reagent 4 Sodium hydroxide (certified) Deionized distilled water 275.0 200.0 200.0 10.8 900.0 mL mL mL g mL Safranin dye solution Safranin 1.0 g Distilled water 100.0 mL Schweppe’s reagent Solution 1 Glucose 2.0 g Distilled 20.0 mL Solution 2 Aniline 2.0 g Ethanol (95%) 20.0 mL Mix solutions 1 and 2 and dilute to 60 mL with alcohol. Always use freshly prepared reagent (can be stored for max. 24 hours at 4-8°C). TRIS [tris(hydroxymethyl)aminomethane] buffer (1M, pH 8.0) Trizma base (Tris base) 121.1 g Double distilled water (fill up to) 1000.0 mL HCl (cc.) 42.2 mL pH: 8.0
2011-0073_practical_microbiology.docx