7i Ph H H Me 11i 51 6-HDNO 55 0 Ph Ph H H 0 7j 11jc 6-HDNO 0 7k Ph H H Ph 11kc 0 0 aFreeze-dried whole cells were used. bConversion values were E.coli measured by GC-MS spectroscopy and/or 1H-NMR. cPyrrolines 7j-k were also treated with MAO-D11 variant, but no conversion was observed. Table 2. MAO-N and 6-HDNO catalysed aromatization of pyrrolines 10a-h and 9 Conv 3-Pyr. Alk R1 R2 R3 Biocat.a Pyrrole (%)b MAO-D5 >99 10a Bn H H H MAO-D9 12a 61 6-HDNO 95 10b 4-Cl-Bn H H H MAO-D5 12b 88 10c H H H MAO-D5 12c >99 Isovaleryl H H H MAO-D5 >99 10d 12d 10e H H H MAO-D5 12e >99 MAO-D5 >99 10f H H H MAO-D9 12f 51 6-HDNO 65
7i Ph H H Me 11i 51 6-HDNO 55 0 7j Ph Ph H H 11jc 0 6-HDNO 0 7k Ph H H Ph 11kc 0 0 aFreeze-dried E.coli whole cells were used. bConversion values were measured by GC-MS spectroscopy and/or 1H-NMR. cPyrrolines 7j-k were also treated with MAO-D11 variant, but no conversion was observed. Table 2. MAO-N and 6-HDNO catalysed aromatization of pyrrolines 10a-h and 9 3-Pyr. Alk R1 R2 R3 Biocat.a Pyrrole Conv (%)b MAO-D5 >99 10a Bn H H H MAO-D9 12a 61 6-HDNO 95 10b 4-Cl-Bn H H H MAO-D5 12b 88 10c H H H MAO-D5 12c >99 10d Isovaleryl H H H MAO-D5 12d >99 10e H H H MAO-D5 12e >99 MAO-D5 >99 10f H H H MAO-D9 12f 51 6-HDNO 65
Scalacci%20et%20al%20-%20Unveiling%20the%20biocatalytic%20aromatizing%20activity%20of%20monoamine%20oxidases%20MAO-N%20and%206-HDNO.docx