20 to 30 min D 30 to 60 min E >60 min F Unknown X Not applicable (e.g. biopsy) N Z Cold ischemia time < 2 min A 2 to 10 min B 10 to 20 min C 20 to 30 min D 30 to 60 min E >60 min F Unknown X Not applicable (e.g. N autopsy) Z Fixation / stabilization type Non-aldehyde with acetic ACA acid Aldehyde-based ALD Allprotect ® tissue reagent ALL Alcohol-based ETH Non-buffered formalin FOR Heat stabilization Snap freezing Non-aldehyde based NAA without acetic acid Neutral buffered formalin NBF Optimum cutting OCT temperature medium PAXgene® tissue PXT RNA Later® RNL Unknown XXX ZZZ Fixation time <15 min A 15 min to 1 h B 1 to 4 h C 4 to 8 h D 8 to 24 h E
20 to 30 min D 30 to 60 min E >60 min F Unknown X Not applicable (e.g. biopsy) N Z Cold ischemia time < 2 min A 2 to 10 min B 10 to 20 min C 20 to 30 min D 30 to 60 min E >60 min F Unknown X Not applicable (e.g. autopsy) N Z Fixation / stabilization type Non-aldehyde with acetic acid ACA Aldehyde-based ALD Allprotect ® tissue reagent ALL Alcohol-based ETH Non-buffered formalin FOR Heat stabilization Snap freezing Non-aldehyde based without acetic acid NAA Neutral buffered formalin NBF Optimum cutting temperature medium OCT PAXgene® tissue PXT RNA Later® RNL Unknown XXX ZZZ Fixation time <15 min A 15 min to 1 h B 1 to 4 h C 4 to 8 h D 8 to 24 h E
SPREC_2.0.docx