laser can be turned on manually or automatically (Recommended: Auto) Shutter mode – (Multiple accum.) removes cosmic ray spikes from spectrum Spike filter – (off) - smooths the data (not recommended) Denoiser (Auto) always on or only during acquisition Laser mode – – not available for 633 nm laser corrects for white light ICS Correction – – Instrument setup StageXY: Marzhauser for regular stage mapping DuoScan for laser only mapping sets the scale bar on the video image Objective – Sets grating Grating – adjusts laser power on sample and CCD ND Filter – (DEFAULT 150)– Adjusts confocal hole diameter in Hole – μm presets internal filters for expected scatter range Range – automatically adjusts spectrometer position when laser/grating are Adjust spectro – changed Map Range mapping will take longer depending on grating and wave Set acquisition settings – number range In Video image, set mapping area Maps Submenu: Set mapping size properties SWIFT Mode option can be used to decrease mapping time not compatible with – range mapping Make sure SWIFT Box (to the left of the lamp) settings are correct Shutdown procedure Save all data in LabSpec Raise objectives Remove sample Turn off lamp Leave LabSpec6 on DISABLE IN BADGER TURN OFF LASERS Do NOT reboot the computer!
Shutter mode – laser can be turned on manually or automatically (Recommended: Auto) Spike filter (Multiple accum.) – removes cosmic ray spikes from spectrum Denoiser (off) - smooths the data (not recommended) Laser mode – (Auto) – always on or only during acquisition ICS Correction – not available for 633 nm laser – corrects for white light Instrument setup StageXY: Marzhauser for regular stage mapping DuoScan for laser only mapping Objective – sets the scale bar on the video image Grating – Sets grating ND Filter – adjusts laser power on sample and CCD Hole (DEFAULT – 150)– Adjusts confocal hole diameter in μm Range – presets internal filters for expected scatter range Adjust spectro – automatically adjusts spectrometer position when laser/grating are changed Map Set acquisition settings – Range mapping will take longer depending on grating and wave number range In Video image, set mapping area Maps Submenu: Set mapping size properties SWIFT Mode option can be used to decrease mapping time – not compatible with range mapping Make sure SWIFT Box (to the left of the lamp) settings are correct Shutdown procedure Save all data in LabSpec Raise objectives Remove sample Turn off lamp Leave LabSpec6 on DISABLE IN BADGER TURN OFF LASERS Do NOT reboot the computer!
SOP_HoribaLabram.docx