Nomenclature and classification; Cofactors; Specificity of enzyme action; Isozymes; Mechanism of enzyme action; Enzyme kinetics; Derivation of Michaelis-Menten equation, Lineweaver-Burk plot; Factors affecting rate of enzyme-catalyzed reactions; Enzyme inhibition; Allosteric enzymes and their kinetics; Strategy of enzyme action- Catalytic and Regulatory (Basic concept with one example each) Unit 6: Oxidative Phosphorylation Redox systems; Review of mitochondrial respiratory chain, Inhibitors and un-couplers of Electron Transport System Reference Cox, M.M and Nelson, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. ► Freeman and Co., New York. Berg, J.M., Tymoczko, J.L. and Stryer, L.(2007). Biochemistry, VI Edition, W.H. Freeman ► and Co., New York. Murray, R.K., Bender, D.A., Botham, K.M., Kennelly, P.J., Rodwell, V.W. and Well, P.A. (2009). ► Harper’s Illustrated Biochemistry, XXVIII Edition, International Edition, The McGraw- Hill Companies Inc. B.D. and Hooper, N.M. (2000). Instant Notes in Biochemistry, II Edition, BIOS Scientific ► Publishers Ltd., U.K. Watson, J.D., Baker, T.A., Bell, S.P., Gann, A., Levine, M. and Losick, R. (2008). Molecular ► Biology of the Gene, VI Edition, Cold Spring Harbor Lab. Press, Pearson Pub. Core P7 of Biochemistry Lab –Fundamentals Fundamentals of Biochemistry 2 Credits List of Practical Qualitative tests of functional groups in carbohydrates, proteins and lipids. Quantitative estimation of Method Demonstration of proteins separation by SDS-PAGE. To the enzymatic activity of Trypsin and Amylase. Preparation of Normal. Molar and Standard solutions, Phosphate Buffers, Serial dilutions
Nomenclature and classification; Cofactors; Specificity of enzyme action; Isozymes; Mechanism of enzyme action; Enzyme kinetics; Derivation of Michaelis-Menten equation, Lineweaver-Burk plot; Factors affecting rate of enzyme-catalyzed reactions; Enzyme inhibition; Allosteric enzymes and their kinetics; Strategy of enzyme action- Catalytic and Regulatory (Basic concept with one example each) Unit 6: Oxidative Phosphorylation Redox systems; Review of mitochondrial respiratory chain, Inhibitors and un-couplers of Electron Transport System Reference ► Cox, M.M and Nelson, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York. ► Berg, J.M., Tymoczko, J.L. and Stryer, L.(2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York. ► Murray, R.K., Bender, D.A., Botham, K.M., Kennelly, P.J., Rodwell, V.W. and Well, P.A. (2009). Harper’s Illustrated Biochemistry, XXVIII Edition, International Edition, The McGraw- Hill Companies Inc. ► B.D. and Hooper, N.M. (2000). Instant Notes in Biochemistry, II Edition, BIOS Scientific Publishers Ltd., U.K. ► Watson, J.D., Baker, T.A., Bell, S.P., Gann, A., Levine, M. and Losick, R. (2008). Molecular Biology of the Gene, VI Edition, Cold Spring Harbor Lab. Press, Pearson Pub. Core P7 –Fundamentals of Biochemistry Lab Fundamentals of Biochemistry 2 Credits List of Practical Qualitative tests of functional groups in carbohydrates, proteins and lipids. Quantitative estimation of Method Demonstration of proteins separation by SDS-PAGE. To the enzymatic activity of Trypsin and Amylase. Preparation of Normal. Molar and Standard solutions, Phosphate Buffers, Serial dilutions
SKBUUGZoology_Hons_prefinal.docx