Budget: $1550 Chemical expenses for the various reagents and solvents acquired from – and Fisher Scientific $750 various chemical markers of melanin – $75 ethyl acetate (1L) – $230 trifluoroacetic acid (100 ml) – $495 Unforeseeable chemical expenses based on potential protocol modification – $750 Purchase of HPLC column from Sigma-Aldrich – $6810 Costs associated with use of mass spectrometry and high performance liquid – chromatography instrumentation at Duke University $6750 total cost for use of HPLC-MS instrument – Reserved for four full (24 hour) days $60 transportation to and from Duke University – 8 days of round-trip travel (80 miles) $1315 Attending and presenting at various regional and national conferences – Southeastern Regional Meeting of the American Chemical Society (SERMACS) Columbia, SC – $80 registration fees – $375 travel costs and hotel accommodation – National Meeting of the American Chemical Society (ACS) San Francisco, CA – $110 Registration fees – $750 Travel costs and hotel accommodation – $4575 Tuition and study abroad fees for the University of Otago – Timeline: Proposed Experiences Proposed Products Spring 2015 Complete two CHM499 research Develop techniques for hours. separating mixtures using SPE and analyzing compounds using Become familiar with required HPLC. laboratory techniques for the analysis of melanin. Establish the appropriate solvents for separating chemical Read scholarly literature and write a markers of melanin. review paper on the physical and chemical properties of melanin. Compose a review paper on melanin for publication. Summer 2015 Depart for New Zealand study Further development of SPE abroad at the end of June. protocols by analyzing preliminary SPE data collected Continue reading scholarly literature in the spring. to remain up-to-date with melanin research. Submit melanin review paper to
Budget: $1550 – Chemical expenses for the various reagents and solvents acquired from and Fisher Scientific $750 – various chemical markers of melanin $75 – ethyl acetate (1L) $230 – trifluoroacetic acid (100 ml) $495 – Unforeseeable chemical expenses based on potential protocol modification $750 – Purchase of HPLC column from Sigma-Aldrich $6810 – Costs associated with use of mass spectrometry and high performance liquid chromatography instrumentation at Duke University $6750 – total cost for use of HPLC-MS instrument Reserved for four full (24 hour) days $60 – transportation to and from Duke University 8 days of round-trip travel (80 miles) $1315 – Attending and presenting at various regional and national conferences Southeastern Regional Meeting of the American Chemical Society (SERMACS) – Columbia, SC $80 – registration fees $375 – travel costs and hotel accommodation National Meeting of the American Chemical Society (ACS) – San Francisco, CA $110 – Registration fees $750 – Travel costs and hotel accommodation $4575 – Tuition and study abroad fees for the University of Otago Timeline: Proposed Experiences Proposed Products Spring 2015 Complete two CHM499 research hours. Become familiar with required laboratory techniques for the analysis of melanin. Read scholarly literature and write a review paper on the physical and chemical properties of melanin. Develop techniques for separating mixtures using SPE and analyzing compounds using HPLC. Establish the appropriate solvents for separating chemical markers of melanin. Compose a review paper on melanin for publication. Summer 2015 Depart for New Zealand study abroad at the end of June. Continue reading scholarly literature to remain up-to-date with melanin research. Further development of SPE protocols by analyzing preliminary SPE data collected in the spring. Submit melanin review paper to
Quantification%20of%20Chemical%20Markers.docx