6. Write down the nucleotide sequence of mRNA encoding the peptide Use A, U, G, and C for unambiguously decided positions, if N1/N2 any of two nucleotides is possible at a particular position, and N if any of four nucleotides is possible at a particular position (N1 and N2 can be any of A, U, G, and C). 7. Molecular weight of an protein is of about 51 kDa. Estimate the length of encoding E.coli mRNA (in nm, rounding to integer). Take the average molecular weight of an amino acid as 110 g/mol, and the average length of a ribonucleotide residue as 0,34 nm. How long will it take a cell to synthesize this protein if the ribosome reads 20 ribonucleotide residues per second? A group of researches accomplished protein synthesis in a cell-free system (in All vitro). required components (ribosomes, tRNAs, ATP, GTP, salts, amino acids, aaRS, translation factors, etc.) were added to the system. A synthetic polyribonucleotide consisting of only A and C in the ration of 1:5 was used as the messenger RNA (nucleotide residues are arranged randomly in the mRNA). 8. Determine the amino acid composition of the synthesized protein. What are the ratios between the amino acid residues in the protein? The 3D structure of a tRNA is depicted in Fig. 2. There are two key regions: the CCA3’ terminus which is linked to the amino acid, and the anticodon exactly matching to the mRNA codon. Fig. 2. The 3D structure of a tRNA 9. A mutant tRNATyr with anticodon specific to Ser codon (instead of Tyr codon) was introduced into the synthetic system described in i.8. What would be the resultant protein?
6. Write down the nucleotide sequence of mRNA encoding the peptide Use A, U, G, and C for unambiguously decided positions, N1/N2 if any of two nucleotides is possible at a particular position, and N if any of four nucleotides is possible at a particular position (N1 and N2 can be any of A, U, G, and C). 7. Molecular weight of an E.coli protein is of about 51 kDa. Estimate the length of encoding mRNA (in nm, rounding to integer). Take the average molecular weight of an amino acid as 110 g/mol, and the average length of a ribonucleotide residue as 0,34 nm. How long will it take a cell to synthesize this protein if the ribosome reads 20 ribonucleotide residues per second? A group of researches accomplished protein synthesis in a cell-free system (in vitro). All required components (ribosomes, tRNAs, ATP, GTP, salts, amino acids, aaRS, translation factors, etc.) were added to the system. A synthetic polyribonucleotide consisting of only A and C in the ration of 1:5 was used as the messenger RNA (nucleotide residues are arranged randomly in the mRNA). 8. Determine the amino acid composition of the synthesized protein. What are the ratios between the amino acid residues in the protein? The 3D structure of a tRNA is depicted in Fig. 2. There are two key regions: the CCA3’ terminus which is linked to the amino acid, and the anticodon exactly matching to the mRNA codon. Fig. 2. The 3D structure of a tRNA 9. A mutant tRNATyr with anticodon specific to Ser codon (instead of Tyr codon) was introduced into the synthetic system described in i.8. What would be the resultant protein?
Preparatory_problems_IChO_2013.docx