Typical working recipes: HUNTaa media 1000ml final volume Yaa media 1000ml final volume same total same total same total mass of mass of mass of amino acid amino acid as amino acid mM biologically mM biologically as in 100N in 200N as in 300N available N 100N all 200N all 300N all available N HUNTaa HUNTaa HUNTaa agar 20 g 20 g 20 g agar 20 g 20 g 20 g 0.58 g 1.16 g 1.74 g L-Ile Powder 0.91 g 1.82 g 2.73 g L-ile Powder 0.82 g 1.64 g 2.46 g L-leu Powder 0.605 g 1.21 g 1.815 g L-leu Powder 0.42 g 0.84 g 1.26 g L-tyr Powder 0.21 g 0.42 g 0.63 g L-tyr Powder 17.12 g 17.12 g 17.12 g sucrose 50mM final 17.12 g 17.12 g 17.12 g sucrose 50mM final 20mg/ml in cholesterol 20mg/ml in EtOH 15 ml 15 ml 15 ml cholesterol EtOH 15 ml 15 ml 15 ml CaCl2 1000x 1 ml 1 ml 1 ml CaCl2 1000x 1 ml 1 ml 1 ml MgSO4 1000x 1 ml 1 ml 1 ml MgSO4 1000x 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml CuSO4 1000x 1 ml 1 ml 1 ml CuSO4 1000x 1 ml 1 ml 1 ml FeSO4 1000x 1 ml 1 ml 1 ml FeSO4 1000x 1 ml 1 ml 1 ml MnCl2 1000x 1 ml 1 ml 1 ml MnCl2 1000x 1 ml 1 ml 1 ml ZnSO4 1000x 1 ml 1 ml 1 ml ZnSO4 1000x Total vol Total vol before before autoclaving 780.9 ml 712.9 ml 644.8 ml autoclaving 776.7 ml 704.5 ml 632.2 ml buffer 10x acetate 100 ml 100 ml 100 ml buffer 10x acetate 100 ml 100 ml 100 ml
Typical working recipes: HUNTaa media 1000ml final volume Yaa media 1000ml final volume mM biologically available N 100N all 200N all 300N all mM biologically available N same total mass of amino acid as in 100N HUNTaa same total mass of amino acid as in 200N HUNTaa same total mass of amino acid as in 300N HUNTaa agar 20 g 20 g 20 g agar 20 g 20 g 20 g L-Ile Powder 0.91 g 1.82 g 2.73 g L-ile Powder 0.58 g 1.16 g 1.74 g L-leu Powder 0.605 g 1.21 g 1.815 g L-leu Powder 0.82 g 1.64 g 2.46 g L-tyr Powder 0.21 g 0.42 g 0.63 g L-tyr Powder 0.42 g 0.84 g 1.26 g sucrose 50mM final 17.12 g 17.12 g 17.12 g sucrose 50mM final 17.12 g 17.12 g 17.12 g cholesterol 20mg/ml in EtOH 15 ml 15 ml 15 ml cholesterol 20mg/ml in EtOH 15 ml 15 ml 15 ml CaCl2 1000x 1 ml 1 ml 1 ml CaCl2 1000x 1 ml 1 ml 1 ml MgSO4 1000x 1 ml 1 ml 1 ml MgSO4 1000x 1 ml 1 ml 1 ml CuSO4 1000x 1 ml 1 ml 1 ml CuSO4 1000x 1 ml 1 ml 1 ml FeSO4 1000x 1 ml 1 ml 1 ml FeSO4 1000x 1 ml 1 ml 1 ml MnCl2 1000x 1 ml 1 ml 1 ml MnCl2 1000x 1 ml 1 ml 1 ml ZnSO4 1000x 1 ml 1 ml 1 ml ZnSO4 1000x 1 ml 1 ml 1 ml Total vol before autoclaving 780.9 ml 712.9 ml 644.8 ml Total vol before autoclaving 776.7 ml 704.5 ml 632.2 ml buffer 10x acetate 100 ml 100 ml 100 ml buffer 10x acetate 100 ml 100 ml 100 ml
Preparation-of-a-holidic-medium-for-Drosophila_120515.docx