Semi-conscious; Not sternally Stage 3 Not less than once every skin color maintain dry respiratory rate adjust ambient 6 hours condition of incision temperature as Conscious; sternally site necessary recumbent; May sit, but evidence of pain consider cannot additional analgesics Stage 2 Twice Daily degree of alertness examine for and activity abnormalities food/water consider Standing and moving consumption additional Not eating/drinking analgesics normally condition of incision consult veterinary site Stage 1 Daily Notation of surgical Remove site care until suture sutures or removal staples as Active and alert; Eating and Record medications indicated drinking normally; Skin administered sutures still in place Stage 1 Observe and record for 1 day if non-surgical procedure Observe and record for 5+ days if minor or major surgical procedure Animal normal; Skin sutures removed Identification Individual rats can be identified using ear punches, ear tags, tattoos, fur dyes and indelible mark on the tail or microchips. A combination of marks made by an ear punch can be used to identify rodents. Rats should always be clearly identified on cage cards indicating protocol number, strain (using standard nomenclature), sex, date of birth, and PI. Euthanasia are a number of acceptable methods for euthanatizing rodents as stated in the most recent Report of the AVMA Panel on Euthanasia and these are listed below. It is important that euthanasia be carried out humanely with a minimum amount of anxiety and pain to the animal. Regardless of the method utilized, UTEP’s IACUC Protocol Application Form states that a secondary method must be used. After euthanasia is performed, death should be assured by bilateral pneumothorax, aortic transection, cervical dislocation, or some other certain physical means, as appropriate. Method Ease of Use Personnel Safety Efficacy
Semi-conscious; Not sternally Stage 3 Conscious; sternally recumbent; May sit, but cannot Not less than once every 6 hours skin color respiratory rate condition of incision site evidence of pain maintain dry adjust ambient temperature as necessary consider additional analgesics Stage 2 Standing and moving Not eating/drinking normally Twice Daily degree of alertness and activity food/water consumption condition of incision site examine for abnormalities consider additional analgesics consult veterinary Stage 1 Active and alert; Eating and drinking normally; Skin sutures still in place Daily Notation of surgical site care until suture removal Record medications administered Remove sutures or staples as indicated Stage 1 Animal normal; Skin sutures removed Observe and record for 1 day if non-surgical procedure Observe and record for 5+ days if minor or major surgical procedure Identification Individual rats can be identified using ear punches, ear tags, tattoos, fur dyes and indelible mark on the tail or microchips. A combination of marks made by an ear punch can be used to identify rodents. Rats should always be clearly identified on cage cards indicating protocol number, strain (using standard nomenclature), sex, date of birth, and PI. Euthanasia are a number of acceptable methods for euthanatizing rodents as stated in the most recent Report of the AVMA Panel on Euthanasia and these are listed below. It is important that euthanasia be carried out humanely with a minimum amount of anxiety and pain to the animal. Regardless of the method utilized, UTEP’s IACUC Protocol Application Form states that a secondary method must be used. After euthanasia is performed, death should be assured by bilateral pneumothorax, aortic transection, cervical dislocation, or some other certain physical means, as appropriate. Method Ease of Use Personnel Safety Efficacy
Rat%20Handling%20and%20Biomethodology%20Handbook%202016.docx