[36] Beleneva, I. A. (2010) Incidence and characteristics of and Staphylococcus aureus from the Japan and South China seas. Mar. Pollut. Bull. doi: Listeria monocytogenes 10.1016/j.marpolbul.2010.09.024. [37] Oliveira, J., A. Cunha, F. Castilho, J. L. Romalde, and M. J. Pereira (2011) Microbial contamination and purification of bivalve shellfish: crucial aspects in monitoring and future perspectives a mini-review. Food Control. 22: 805-816. – Table 1. Biochemical composition (% w/w) of the native sample and the CACR fraction extracted from Chaetomorpha aerea. Uronic Sulfated Composition (% w/w) Carbohydrates Proteins Others acids carbohydrates 3.82 ± 0.12 nd 2.30 ± 0.11 0.33 ± 0.14 93.9 Control fraction Partially purified 28.1 ± 2.31 nd 6.53 ± 1.08 2.21 ± 0.92 71.9 fraction CACR fraction 76.6 ± 0.62 nd 17.3 ± 2.85 6.3 ± 1.2 5.8 nd: non determined by colorimetric assays ±: Standard deviation on 10 runs. Table 2. Identification and quantification by GC/MS of the monosaccharides composing the CACR fraction extracted from Chaetomorpha aerea. Retention time Retention time Homology MS Concentration Monosaccharides of peaks in the of standards spectra (%) (% w/w) CA fraction 6.49 - - - Rhamnose Arabinose 6.61 6.59 93.45 18 Fucose 7.65 - - - Xylose 8.59 9.11 91.87 trace
[36] Beleneva, I. A. (2010) Incidence and characteristics of Staphylococcus aureus and Listeria monocytogenes from the Japan and South China seas. Mar. Pollut. Bull. doi: 10.1016/j.marpolbul.2010.09.024. [37] Oliveira, J., A. Cunha, F. Castilho, J. L. Romalde, and M. J. Pereira (2011) Microbial contamination and purification of bivalve shellfish: crucial aspects in monitoring and future perspectives – a mini-review. Food Control. 22: 805-816. Table 1. Biochemical composition (% w/w) of the native sample and the CACR fraction extracted from Chaetomorpha aerea. Composition (% w/w) Carbohydrates Uronic acids Proteins Sulfated carbohydrates Others Control fraction 3.82 ± 0.12 nd 2.30 ± 0.11 0.33 ± 0.14 93.9 Partially purified fraction 28.1 ± 2.31 nd 6.53 ± 1.08 2.21 ± 0.92 71.9 CACR fraction 76.6 ± 0.62 nd 17.3 ± 2.85 6.3 ± 1.2 5.8 nd: non determined by colorimetric assays ±: Standard deviation on 10 runs. Table 2. Identification and quantification by GC/MS of the monosaccharides composing the CACR fraction extracted from Chaetomorpha aerea. Monosaccharides Retention time of standards Retention time of peaks in the CA fraction Homology MS spectra (%) Concentration (% w/w) Rhamnose 6.49 - - - Arabinose 6.61 6.59 93.45 18 Fucose 7.65 - - - Xylose 8.59 9.11 91.87 trace
Pub_Biotech_Bioproc_Engin_final_GP.docx