28 0.4 10 mM 1.3 × 107 phosphate / 7.2 10 mM Tris, 100 0.5 2.7 × 107 mM NaCl / 7.3 20 mM Tris, 100 1.2 2.9 × 107 mM NaCl / 7.3 50 mM Tris, 100 3.8 4.2 × 107 mM NaCl / 7.3 100 mM Tris, 30 1.9 × 107 100 mM NaCl / 7.3 40 20 mM Hepes, [68] 0.6 2.8 × 107 100 mM NaCl, pH 7.4 50 mM Hepes, 0.9 4.2 × 107 100 mM NaCl, / 7.4 100 mM Hepes, 2.5 2.9 × 107 100 mM NaCl / 7.4 42 20 mM 0.8 2.1 × 107 100 mM NaCl / 7.4 40 20-100 mM 1.8 × 107 [75] Hepes / 7.4 Table 5. Summary of stability constants for Cu(II)-Aβ complexes, obtained from ITC and potentiometric data (ref. [80]). The and cKML values are given as provided in the Kdapp original papers, except for ref. [80], where cKML values were calculated from the source log constants by the CI approach [41, 42]. β Aβ peptide cKML (M-1) Competitor ref. Kdapp Buffer / pH (μM) 1.1 × 1010 20 mM Hepes / 7.2 Gly [76] 40 2.4 × 1010 20 mM Hepes /7.4 Gly 9 × 1010 20 mM Pipes / 7.2 Gly 1.5 × 1010 20 mM Hepes / 7.2 Gly 16 2.9 × 1010 20 mM Hepes /7.4 Gly 3.0 × 1010 20 mM Pipes / 7.4 Gly 16 1.5 × 1010 20 mM Hepes / 7.2 Gly [77] 16 0.1 50 mM 100 mM - NaCl / 7.4 28 0.1 50 mM 100 mM - NaCl / 7.4
28 0.4 1.3 × 107 10 mM phosphate / 7.2 40 0.5 2.7 × 107 10 mM Tris, 100 mM NaCl / 7.3 [68] 1.2 2.9 × 107 20 mM Tris, 100 mM NaCl / 7.3 3.8 4.2 × 107 50 mM Tris, 100 mM NaCl / 7.3 30 1.9 × 107 100 mM Tris, 100 mM NaCl / 7.3 0.6 2.8 × 107 20 mM Hepes, 100 mM NaCl, pH 7.4 0.9 4.2 × 107 50 mM Hepes, 100 mM NaCl, / 7.4 2.5 2.9 × 107 100 mM Hepes, 100 mM NaCl / 7.4 42 0.8 2.1 × 107 20 mM 100 mM NaCl / 7.4 40 1.8 × 107 20-100 mM Hepes / 7.4 [75] Table 5. Summary of stability constants for Cu(II)-Aβ complexes, obtained from ITC and potentiometric data (ref. [80]). The Kdapp and cKML values are given as provided in the original papers, except for ref. [80], where cKML values were calculated from the source log β constants by the CI approach [41, 42]. Aβ peptide Kdapp (μM) cKML (M-1) Buffer / pH Competitor ref. 40 1.1 × 1010 20 mM Hepes / 7.2 Gly [76] 2.4 × 1010 20 mM Hepes /7.4 Gly 9 × 1010 20 mM Pipes / 7.2 Gly 16 1.5 × 1010 20 mM Hepes / 7.2 Gly 2.9 × 1010 20 mM Hepes /7.4 Gly 3.0 × 1010 20 mM Pipes / 7.4 Gly 16 1.5 × 1010 20 mM Hepes / 7.2 Gly [77] 16 0.1 50 mM 100 mM NaCl / 7.4 - 28 0.1 50 mM 100 mM NaCl / 7.4 -
RevCCRrev.docx