buffer base buffer base 8 ml 8 ml 8 ml nucl/lipid soln 125x stock 8 ml 8 ml 8 ml nucl/lipid soln 125x stock essential amino essential amino HUNTaa acid stock acid stock 30.26 ml 60.51 ml 90.77 ml solutions solution (EAA) 30.26 ml 60.51 ml 90.77 ml Yaa solutions solution (EAA) non essential non essential amino acid amino acid stock stock solution 30.26 ml 60.51 ml 90.77 ml solution (NEAA) 30.26 ml 60.51 ml 90.77 ml (NEAA) Na glutamate Na glutamate solution solution 9.11 ml 18.21 ml 27.32 ml (100mg/ml) 7.57 ml 15.13 ml 22.70 ml (100mg/ml) Cys solution 2.64 ml 5.28 ml 7.92 ml (50mg/ml) Vitamin stock 47.6x stock 21 ml 21 ml 21 ml Vitamin stock 47.6x stock 21 ml 21 ml 21 ml 1 ml 1 ml 1 ml folic acid stock 1000x stock 1 ml 1 ml 1 ml folic acid stock 1000x stock 6 ml 6 ml 6 ml Propionic acid 6 ml 6 ml 6 ml Propionic acid 100 g/l stock in 100 g/l stock in 15 ml 15 ml 15 ml Nipagin 95% EtOH 15 ml 15 ml 15 ml Nipagin 95% EtOH
buffer base buffer base nucl/lipid soln 125x stock 8 ml 8 ml 8 ml nucl/lipid soln 125x stock 8 ml 8 ml 8 ml HUNTaa solutions essential amino acid stock solution (EAA) 30.26 ml 60.51 ml 90.77 ml Yaa solutions essential amino acid stock solution (EAA) 30.26 ml 60.51 ml 90.77 ml non essential amino acid stock solution (NEAA) 30.26 ml 60.51 ml 90.77 ml non essential amino acid stock solution (NEAA) 30.26 ml 60.51 ml 90.77 ml Na glutamate solution (100mg/ml) 7.57 ml 15.13 ml 22.70 ml Na glutamate solution (100mg/ml) 9.11 ml 18.21 ml 27.32 ml Cys solution (50mg/ml) 2.64 ml 5.28 ml 7.92 ml Vitamin stock 47.6x stock 21 ml 21 ml 21 ml Vitamin stock 47.6x stock 21 ml 21 ml 21 ml folic acid stock 1000x stock 1 ml 1 ml 1 ml folic acid stock 1000x stock 1 ml 1 ml 1 ml Propionic acid 6 ml 6 ml 6 ml Propionic acid 6 ml 6 ml 6 ml Nipagin 100 g/l stock in 95% EtOH 15 ml 15 ml 15 ml Nipagin 100 g/l stock in 95% EtOH 15 ml 15 ml 15 ml
Preparation-of-a-holidic-medium-for-Drosophila_120515.docx