Author/s Study design Number of Visualisation methods Chronic wound aetiologies No of samples with participants with or without molecular confirmed biofilm (%) James et al (2008) [24] Prospective study 66 Light microscopy, SEM 13 DFUs, 21 PUs 30 out of 50 case vs control 16S rRNA with DGGE 8 VLUs, 24 NHSW (60%) Kirketerp-Moller (2008) [39] Prospective cohort 22 PNA-FISH, CLSM Un-specified chronic wounds 13 of 22 study (60%) 9 PNA-FISH, CLSM 10 VLUs 10 of 10 Fazli et al (2009) [42] Prospective cohort (100%) study PNA-FISH, 16S rRNA 2 VLUs 2 of 2 Thomsen et al (2009) [44] Prospective cohort 2 (100%) study Sub analysis 15 PNA-FISH, CLSM 4 DFUs, 5 PUs, 2 VLUs 9 of 15 Han et al (2011) [38] Prospective cohort 16S rRNA 4 NHSW (60%) study Neut et al (2011) [43] Case report 2 CLSM 2 DFUs 2 of 2 (100%) 4 FISH, SEM, 4 DFUs 4 of 4 Oates et al (2014) [40] Prospective cohort (100%)
Author/s Study design Number of participants Visualisation methods with or without molecular Chronic wound aetiologies No of samples with confirmed biofilm (%) James et al (2008) [24] Prospective study case vs control 66 Light microscopy, SEM 16S rRNA with DGGE 13 DFUs, 21 PUs 8 VLUs, 24 NHSW 30 out of 50 (60%) Kirketerp-Moller (2008) [39] Prospective cohort study 22 PNA-FISH, CLSM Un-specified chronic wounds 13 of 22 (60%) Fazli et al (2009) [42] Prospective cohort study 9 PNA-FISH, CLSM 10 VLUs 10 of 10 (100%) Thomsen et al (2009) [44] Prospective cohort study Sub analysis 2 PNA-FISH, 16S rRNA 2 VLUs 2 of 2 (100%) Han et al (2011) [38] Prospective cohort study 15 PNA-FISH, CLSM 16S rRNA 4 DFUs, 5 PUs, 2 VLUs 4 NHSW 9 of 15 (60%) Neut et al (2011) [43] Case report 2 CLSM 2 DFUs 2 of 2 (100%) Oates et al (2014) [40] Prospective cohort 4 FISH, SEM, 4 DFUs 4 of 4 (100%)
Prevalence_of_biofilm_in_chronic_wounds_V7_final_TB_am.docx