identify genes within the sequence, and when compared to one or more related, annotated, and preferably finished genomes, can suggest which contigs are adjacent to each other and possibly overlapping. Procedure 1. Use Firefox to login to the RAST website (). Click “Your Jobs” “Upload New Job”. On the subsequent page, Browse to the Assembly2.fasta file from the previous section, then click “Use the data and go to step 2 2. Open a new tab in your browser, go to the NCBI website () and perform a Taxonomy search for the organism. Copy the NCBI taxonomy ID into the appropriate box on the RAST page and click the lookup button. Click “Use this data and go to step 3” 3. Enter the requested information, change the FIGfam version to the highest number, check “build metabolic model”, then click “Finish the upload” 4. In a new browser tab, Go to and search for your organism’s genus. 5. Click on the link for an organism of interest (closest relatives), then the INSDC link or the RefSeq link followed by the wgs link. Download the GenBank file, unzip (twice), and upload to RAST for annotation. Day 3 – Wednesday June 4, 2014 - Session 2b - 10:30-12:00 –DNA Quality Control
identify genes within the sequence, and when compared to one or more related, annotated, and preferably finished genomes, can suggest which contigs are adjacent to each other and possibly overlapping. Procedure 1. Use Firefox to login to the RAST website (). Click “Your Jobs” “Upload New Job”. On the subsequent page, Browse to the Assembly2.fasta file from the previous section, then click “Use the data and go to step 2 2. Open a new tab in your browser, go to the NCBI website () and perform a Taxonomy search for the organism. Copy the NCBI taxonomy ID into the appropriate box on the RAST page and click the lookup button. Click “Use this data and go to step 3” 3. Enter the requested information, change the FIGfam version to the highest number, check “build metabolic model”, then click “Finish the upload” 4. In a new browser tab, Go to and search for your organism’s genus. 5. Click on the link for an organism of interest (closest relatives), then the INSDC link or the RefSeq link followed by the wgs link. Download the GenBank file, unzip (twice), and upload to RAST for annotation. Day 3 – Wednesday June 4, 2014 - Session 2b - 10:30-12:00 –DNA Quality Control
ProkaryoticGenomicsBreakoutSession2014.docx