SUMMARY OF RECOMMENDED BIOSFAETY LEVELS FOR ACTIVITIES IN WHICH EXPERIMENTALLY OR NATURALLY INFECTED VERTBRATE ANIMALS ARE USED ABSL Agents Practices Primary Barriers and Safety Facilities Equipment (Secondary Barriers) 1 Not known to Standard animal care and As required for normal care of each Standard animal facility consistently cause management practices, including species No recirculation of exhaust disease in healthy adults appropriate medical surveillance air programs Directional air flow recommended Hand washing sink is available 2 Associated with human disease ABSL-1 practice plus: ABSL-1 equipment plus primary barriers: ABSL-1 plus: Hazard percutaneous exposure, Limited access Containment equipment appropriate Autoclave available ingestion, mucous membrane Biohazard warning signs for animal species Handwashing sink available exposure precautions PPEs*: laboratory coats; gloves; face Mechanical cage washer “Sharps” Biosafety manual protection as needed recommended Decontamination of all infectious wastes and of animal cages prior to washing 3 Indigenous or exotic agents with ABSL-2 practice plus: ABSL-2 equipment plus: ABSL-2 facility plus: potential for aerosol Controlled access Containment equipment for housing Physical separation from transmission; Decontamination of clothing before animals and cage dumping activities access corridors Disease may have serious health laundering I, II or III BSC’s available for Self-closing, double-door effects decontaminated before manipulative procedures (inoculation, access bedding removed necropsy) that may create infectious Sealed penetrations Disinfectant foot bath as needed aerosols Sealed windows PPEs*: Autoclave available in facility Appropriate respiratory protection 4 Dangerous/exotic agents that ABSL-3 practices plus: ABSL-3 equipment plus: ABSL-3 plus: pose high risk of life-threatening Entrance through change room Maximum containment equipment Separate building or isolated disease where personal clothing is (i.e., Class III BSC or partial zone Aerosol transmission, or related removed and laboratory clothing is containment equipment in Dedicated supply and agents with unknown risk of put on; shower on exiting. combination with full body, exhaust, vacuum, and transmission All wastes are decontaminated positive-pressure personnel decontamination systems before removal from the facility suit) used for all procedures and requirements outlined in activities the text *PPE-Personal Protective Equipment
SUMMARY OF RECOMMENDED BIOSFAETY LEVELS FOR ACTIVITIES IN WHICH EXPERIMENTALLY OR NATURALLY INFECTED VERTBRATE ANIMALS ARE USED ABSL Agents Practices Primary Barriers and Safety Equipment Facilities (Secondary Barriers) 1 Not known to consistently cause disease in healthy adults Standard animal care and management practices, including appropriate medical surveillance programs As required for normal care of each species Standard animal facility No recirculation of exhaust air Directional air flow recommended Hand washing sink is available 2 Associated with human disease Hazard percutaneous exposure, ingestion, mucous membrane exposure ABSL-1 practice plus: Limited access Biohazard warning signs “Sharps” precautions Biosafety manual Decontamination of all infectious wastes and of animal cages prior to washing ABSL-1 equipment plus primary barriers: Containment equipment appropriate for animal species PPEs*: laboratory coats; gloves; face protection as needed ABSL-1 plus: Autoclave available Handwashing sink available Mechanical cage washer recommended 3 Indigenous or exotic agents with potential for aerosol transmission; Disease may have serious health effects ABSL-2 practice plus: Controlled access Decontamination of clothing before laundering decontaminated before bedding removed Disinfectant foot bath as needed ABSL-2 equipment plus: Containment equipment for housing animals and cage dumping activities I, II or III BSC’s available for manipulative procedures (inoculation, necropsy) that may create infectious aerosols PPEs*: Appropriate respiratory protection ABSL-2 facility plus: Physical separation from access corridors Self-closing, double-door access Sealed penetrations Sealed windows Autoclave available in facility 4 Dangerous/exotic agents that pose high risk of life-threatening disease Aerosol transmission, or related agents with unknown risk of transmission ABSL-3 practices plus: Entrance through change room where personal clothing is removed and laboratory clothing is put on; shower on exiting. All wastes are decontaminated before removal from the facility ABSL-3 equipment plus: Maximum containment equipment (i.e., Class III BSC or partial containment equipment in combination with full body, positive-pressure personnel suit) used for all procedures and activities ABSL-3 plus: Separate building or isolated zone Dedicated supply and exhaust, vacuum, and decontamination systems requirements outlined in the text *PPE-Personal Protective Equipment
Registration-for-the-use-of-Biohazardous-Agents-1-26-17.docx