WT 2.3 × 109 20 mM 7.2 A53T 2.4 × 109 PIPES, 103 A30P 1.6 × 109 37 70-90 ITC mM NaCl, 4 [61] WT 4.7 × 109 mol. eq. of A53T 5.0 × 109 7.4 Gly per Cu(II) A30P 3.6 × 109 0.11 MDVFMK 20 mM Hepes, 0.11 WT 7.5 15 70 ITC [59] 100 mM NaCl 0.12 H50A 0.12 20 mM Mes, WT 6.5 15 300 CD [57] 100 mM NaCl 0.10 20 mM F4W fluorescence 0.11 7.0 25 MOPS, 100 [58] F4W/H50S decay mM NaCl WT 9.96 × 109 EPR, pentaGly [55] H50A 2.5 × 109 competition 100 mM aS1-39 4 × 108 7.4 25 potentiometry [62] KNO3 Table 4. Summary of stability constants for Cu(II)-Aβ complexes, obtained from Tyr-10 fluorescence data. The cKML values were calculated from the literature data by Tõugu et al. [68], Kdapp except for ref. [75]. Aβ Kdapp (μM) cKML (M-1) Buffer / pH ref. peptide 40 10 mM Tris, 100 1.6 1.4 × 108 mM NaCl / 7.4 - [69] 42 10 mM Tris, 100 2.0 1.1 × 108 mM NaCl / 7.4 28 Gly/His 0.01-0.1 107 - 108 H2O / 7.8 [70] 100 mM Tris, 28 28 3.1 × 107 150 mM NaCl / [71] 40 11 8.3 × 107 7.4 40 50 mM PBS, 100 8 3.1 × 106 [72] mM NaCl / 7.4 16 Gly/His ~ 0.1 107 H2O / 7.8 [73] 28 2.5 10 mM Hepes / 2.7 × 107 7.2
WT 2.3 × 109 7.2 37 70-90 ITC 20 mM PIPES, 103 mM NaCl, 4 mol. eq. of Gly per Cu(II) [61] A53T 2.4 × 109 A30P 1.6 × 109 WT 4.7 × 109 7.4 A53T 5.0 × 109 A30P 3.6 × 109 MDVFMK 0.11 7.5 15 70 ITC 20 mM Hepes, 100 mM NaCl [59] WT 0.11 H50A 0.12 WT 0.12 6.5 15 300 CD 20 mM Mes, 100 mM NaCl [57] F4W F4W/H50S 0.10 0.11 7.0 25 fluorescence decay 20 mM MOPS, 100 mM NaCl [58] WT H50A 9.96 × 109 2.5 × 109 EPR, pentaGly competition [55] aS1-39 4 × 108 7.4 25 potentiometry 100 mM KNO3 [62] Table 4. Summary of stability constants for Cu(II)-Aβ complexes, obtained from Tyr-10 fluorescence data. The cKML values were calculated from the literature Kdapp data by Tõugu et al. [68], except for ref. [75]. Aβ peptide Kdapp (μM) cKML (M-1) Buffer / pH ref. 40 1.6 1.4 × 108 10 mM Tris, 100 mM NaCl / 7.4 - [69] 42 2.0 1.1 × 108 10 mM Tris, 100 mM NaCl / 7.4 28 0.01-0.1 107 - 108 H2O / 7.8 Gly/His [70] 28 28 3.1 × 107 100 mM Tris, 150 mM NaCl / 7.4 [71] 40 11 8.3 × 107 40 8 3.1 × 106 50 mM PBS, 100 mM NaCl / 7.4 [72] 16 ~ 0.1 107 H2O / 7.8 Gly/His [73] 28 2.5 2.7 × 107 10 mM Hepes / 7.2
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