How does the intensity of the fluorescent Intensity is proportional to the sequence homology at signal maintain a particular location? a particular locus. How are (SNPs) single-nucleotide They are identified with the set of SNPs that are polymorphisms detected? directly related to or that cause disease. How can diseases such as leukemias, Detection of point mutations can be used for tumors, and characterizing microbial classifying these diseases. agents be distinguished? How is it determined on low sensitive Techniques are determined by conditions of the and specific hybridization techniques? reaction itself. How must two nucleic acid strands keep Nucleic acid strands must share at least 16-20 a perfect complementarity to form a consecutive bases. stable hybrid? What must be considered with Conditions such as stringency, or correct pairing. amplification? How is stringency affected? Stringency is affected by salt concentration temperature, and the concentration of D’s stabilizing agents such as urea. What is DNA sequencing? DNA sequencing is considered the standard” “gold for many molecular applications. How do you provide greater accurancy The sequence should be analyzed on both DNA for both DNA strands? strands. How are mutations detected? Patient sequences are compared to known reference sequences. Why is a PCR amplification include It includes a first step to amplify the region of most sequence strategies? interest to be sequenced. What is the name of the reaction to The reaction is on dideoxy chain termination sequencing? reaction developed by Sanger and collegues in 1977. What happens when one of the four Synthesis is halted. dideoxynucleotides are incorporated into a growing DNA What type of ring does these nucleotides The pentose ring. lack the 3’ and 2’ hydroxyl (OH) group? What happens when you try to It terminates the length incorporate dideoxynucleotide to elongate the DNA chain? What is the most commonly used for sequencing DNA sequencing, similar to PCR? What are the steps that are involved in The steps involve denaturing, annealing of a primer, cycle sequencing? chain extending, and terminating by varying the temperature of the reaction. What are generated fragments They are tagged with fluorescent dye and separated, tagged with? based on size, by denaturing gel or capillary electrophoresis. What are separated fragments spotted They are spotted by fluorescence detectors as the by? fragments pass through the detector.
How does the intensity of the fluorescent signal maintain a particular location? Intensity is proportional to the sequence homology at a particular locus. How are (SNPs) single-nucleotide polymorphisms detected? They are identified with the set of SNPs that are directly related to or that cause disease. How can diseases such as leukemias, tumors, and characterizing microbial agents be distinguished? Detection of point mutations can be used for classifying these diseases. How is it determined on low sensitive and specific hybridization techniques? Techniques are determined by conditions of the reaction itself. How must two nucleic acid strands keep a perfect complementarity to form a stable hybrid? Nucleic acid strands must share at least 16-20 consecutive bases. What must be considered with amplification? Conditions such as stringency, or correct pairing. How is stringency affected? Stringency is affected by salt concentration temperature, and the concentration of D’s stabilizing agents such as urea. What is DNA sequencing? DNA sequencing is considered the “gold standard” for many molecular applications. How do you provide greater accurancy for both DNA strands? The sequence should be analyzed on both DNA strands. How are mutations detected? Patient sequences are compared to known reference sequences. Why is a PCR amplification include most sequence strategies? It includes a first step to amplify the region of interest to be sequenced. What is the name of the reaction to sequencing? The reaction is on dideoxy chain termination reaction developed by Sanger and collegues in 1977. What happens when one of the four dideoxynucleotides are incorporated into a growing DNA Synthesis is halted. What type of ring does these nucleotides lack the 3’ and 2’ hydroxyl (OH) group? The pentose ring. What happens when you try to incorporate dideoxynucleotide to elongate the DNA chain? It terminates the length What is the most commonly used for DNA sequencing, similar to PCR? sequencing What are the steps that are involved in cycle sequencing? The steps involve denaturing, annealing of a primer, chain extending, and terminating by varying the temperature of the reaction. What are generated fragments tagged with? They are tagged with fluorescent dye and separated, based on size, by denaturing gel or capillary electrophoresis. What are separated fragments spotted by? They are spotted by fluorescence detectors as the fragments pass through the detector.
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