Characteristics of TaqMan qPCR primer/probe sets used in this study. Table S4. Primer/probe Target Sequence (5′-3′) Position E. Tm b GC (%) Amplicon References namea coli no (oC) size (bp) Arc-787F(F) Archaea ATTAGATACCCSBGTAGTCC 787–806 61.0 45 273 (Yu et al Arc-915P(P) AGGAATTGGCGGGGGAGCAC 915–934 70.1 65 2005) Arc-1059R(R) GCCATGCACCWCCTCT 1044–1059 62.3 63 Mst-702F(F) Methanosaeta TAATCCTTGAAGGACCACCA 702-721 61.0 45 161 (Yu et al Mst-753P(P) ACGGCAAGGGACGAAAGCTAGG 753-774 70.0 59 2005) Mst-862R(R) CCTACGGCACCGACAAC 846-862 62.0 65 Msc-586F(F) Methanosarcina CGGTTTGGTCAGTCCTCCG 586-604 61.6 63 257 (Chen and Msc-743P(P) AACGGGTTCGACGGTGAGGGACGA 743-766 70.6 63 He 2015) Msc-842R(R) ACCAGACACGGTCGCGC 826-842 59.8 71 aDesignations in the parentheses: F - forward primer, R - reverse primer, and P - probe bMelting temperature estimated using the Oligo Calculator (http://www.basic.northwestern.edu/biotools/oligocalc.html)
Table S4. Characteristics of TaqMan qPCR primer/probe sets used in this study. Primer/probe namea Target Sequence (5′-3′) Position E. coli no Tm b (oC) GC (%) Amplicon size (bp) References Arc-787F(F) Arc-915P(P) Arc-1059R(R) Archaea ATTAGATACCCSBGTAGTCC AGGAATTGGCGGGGGAGCAC GCCATGCACCWCCTCT 787–806 915–934 1044–1059 61.0 70.1 62.3 45 65 63 273 (Yu et al 2005) Mst-702F(F) Mst-753P(P) Mst-862R(R) Methanosaeta TAATCCTTGAAGGACCACCA ACGGCAAGGGACGAAAGCTAGG CCTACGGCACCGACAAC 702-721 753-774 846-862 61.0 70.0 62.0 45 59 65 161 (Yu et al 2005) Msc-586F(F) Msc-743P(P) Msc-842R(R) Methanosarcina CGGTTTGGTCAGTCCTCCG AACGGGTTCGACGGTGAGGGACGA ACCAGACACGGTCGCGC 586-604 743-766 826-842 61.6 70.6 59.8 63 63 71 257 (Chen and He 2015) aDesignations in the parentheses: F - forward primer, R - reverse primer, and P - probe bMelting temperature estimated using the Oligo Calculator (http://www.basic.northwestern.edu/biotools/oligocalc.html)
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