Grey, mucoid colonies that Very similar to colonies of Enterobacter spp. - + LF - R coalesce. Klebsiella spp. Motile. 2-4mm Characteristic swarming on non-selective agar Grey, swarming which can be in waves. Proteus spp. growth over the - + NLF - R Turns BTA brown. Very agar. foul smell. Colonies pale and discreet on MAC, but edges may be irregular. Grey or Pseudomonas yellowish-green, Some may produce the spp. other than flat and - + NLF - R yellowish-green pigment, spreading. pyoverdin. P. aeruginosa 2.5-4mm Grey-white, Colonies older than 48 Corynebacterium round and moist hours become drier. - - + R renale 0.5-1 mm Urease Some brucellae require Translucent, 10% CO2 for growth. convex and Brucella spp. - - - R(C) Colonies not visible until round 3 days after incubation. 0.5 mm MZN positive. Requires 2-3 days for Small, growth reduced round and R Campylobacter - - - oxygen tension. Curved opaque fetus curved rods, if in pairs they have 0.5 mm a "seagull" appearance Grey to translucent, Actinobacillus spp. - V V - R Usually urease positive round and shiny 0.5-1mm Translucent, Colonies appear pinkish smooth, round Pasteurella on BTA. Characteristic - - - R and shiny multocida sweetish Indole positive. 1-2 mm Colonies very small at 24 Small Bordetella hours but become much and round - + NLF - R bronchiseptica larger later. Unreactive 0.5-2 mm bacterium. Salmon pink and The colour becomes more mucoid. Rhodococcus equi - - + R(C) definite with time. Mucoid Colonies colonies tend to merge. coalesce White, shiny, Staphylococcus Colonies are similar to round and LF epidermidis coagulase + - + + C convex (coagulase staphylococci, but always Most negative) white and non-haemolytic. 2-3 mm Colonies are usually White, yellow, Micrococcus spp. - - + C pigmented. tan or pink.
Enterobacter spp. Grey, mucoid colonies that coalesce. 2-4mm - + LF - R Very similar to colonies of Klebsiella spp. Motile. Proteus spp. Grey, swarming growth over the agar. - + NLF - R Characteristic swarming on non-selective agar which can be in waves. Turns BTA brown. Very foul smell. Colonies pale and discreet on MAC, but edges may be irregular. Pseudomonas spp. other than P. aeruginosa Grey or yellowish-green, flat and spreading. 2.5-4mm - + NLF - R Some may produce the yellowish-green pigment, pyoverdin. Corynebacterium renale Grey-white, round and moist 0.5-1 mm - - + R Colonies older than 48 hours become drier. Urease Brucella spp. Translucent, convex and round 0.5 mm - - - R(C) Some brucellae require 10% CO2 for growth. Colonies not visible until days after incubation. MZN positive. Campylobacter fetus Small, round and opaque 0.5 mm - - - R curved Requires 2-3 days for growth reduced oxygen tension. Curved rods, if in pairs they have a "seagull" appearance Actinobacillus spp. Grey to translucent, round and shiny 0.5-1mm - V V - R Usually urease positive Pasteurella multocida Translucent, smooth, round and shiny 1-2 mm - - - R Colonies appear pinkish on BTA. Characteristic sweetish Indole positive. Bordetella bronchiseptica Small and round 0.5-2 mm - + NLF - R Colonies very small at 24 hours but become much larger later. Unreactive bacterium. Rhodococcus equi Salmon pink and mucoid. Colonies coalesce - - + R(C) The colour becomes more definite with time. Mucoid colonies tend to merge. Staphylococcus epidermidis (coagulase negative) White, shiny, round and convex 2-3 mm - + LF Most + C Colonies are similar to coagulase + staphylococci, but always white and non-haemolytic. Micrococcus spp. White, yellow, tan or pink. - - + C Colonies are usually pigmented. 3
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