Soil Type(s): None Flexible: Can Inoculated broth 4.5 mL of 0.2 mL of Beef broth is Bovine serum Inoculated Inoculated be chosen inoculums in inoculum in broth broth “moderate” on the nutrient broth nutrient broth soil, condition of Hamburger is use soil “heavy” Soil Load Volume of 0.3g/L clean 10 µl of 4.5 mL of 0.2 mL of 4.5 mL of 5% in the Amount of None (Quantity): the conditions; inoculated broth inoculums in inoculum in Beef broth virus inoculated specifically inoculum in for tube 1 and Nutrient broth nutrient broth for moderate inoculum broth which added. Just 3 g/L dirty Nutrient total 100 µl for soil ends up on the dried TSB conditions broth used tube 10 hands during from Handling immersion of inoculating contaminated the hands broth hamburger for 2 min Method of Inoculation Inoculation Inoculation of 3 -1.5 mL of an of a 4.5 mL of 1.5 mL of the Immersion into Immersion Contamination: of the of the the product overnight concentrated Beef broth suspension, seeded broth into seeded product product broth culture broth suspension for moderate 90 sec spread, broth of the test of the test soil 90 sec dry organism organism Handling Or 20µL of contaminated virus hamburger suspension on for 2 min each finger tip Baseline Not 1.5x108-5x N/A 5x108-1x109 cfu/hand Suspension The virus Inoculum Inoculum ≥108 Recovery specified 108 (Usually 8.5-9.0 1x108 shall 2x108-2x 109 2x108-2x “pull” Liquid Value): log10 cfu/hand) contain 109 ≥107 suspension Log pre-values infective used for at least 5 Log unit/mL contamination. at
Soil Type(s): None Flexible: Can be chosen on the condition of use Inoculated broth 4.5 mL of inoculums in nutrient broth 0.2 mL of inoculum in nutrient broth Beef broth is “moderate” soil, Hamburger is “heavy” soil Bovine serum Inoculated broth Inoculated broth Soil Load (Quantity): Volume of the inoculum in Nutrient broth used 0.3g/L clean conditions; 3 g/L dirty conditions 10 µl of inoculated broth for tube 1 and total 100 µl for tube 10 4.5 mL of inoculums in Nutrient broth 0.2 mL of inoculum in nutrient broth 4.5 mL of Beef broth for moderate soil Handling contaminated hamburger for 2 min 5% in the virus inoculum Amount of inoculated broth which ends up on the hands during immersion of the hands None specifically added. Just dried TSB from inoculating broth Method of Contamination: Inoculation of the product Inoculation of the product Inoculation of the product 3 -1.5 mL of an overnight broth culture of the test organism of a concentrated broth suspension of the test organism 4.5 mL of Beef broth for moderate soil Handling contaminated hamburger for 2 min 1.5 mL of the suspension, 90 sec spread, 90 sec dry Or 20µL of virus suspension on each finger tip Immersion into seeded broth Immersion into seeded broth Baseline Recovery Value): Not specified 1.5x108-5x 108 N/A 5x108-1x109 Liquid suspension used for contamination. ≥108 cfu/hand (Usually 8.5-9.0 log10 cfu/hand) Suspension 1x108 The virus “pull” shall contain ≥107 infective unit/mL Inoculum 2x108-2x 109 Log pre-values at least 5 Inoculum 2x108-2x 109 Log at
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