Discussion In this chapter I developed and optimized a co-culture system to study the interactions of MM cells with the osteoblasts via adhesion molecule, N-cadherin, 5T33MM in vitro. cells and 5TGM1 cells were cultured separately as described in methods. Mouse in vitro primary osteoblasts were obtained from calvarial bones of 2-4 day old C57Bl/6J mice, (Harlan, UK) and cultured as described in methods. Osteoblasts were in vitro differentiated into mature functional osteoblasts using an model of in vitro
Discussion In this chapter I developed and optimized a co-culture system to study the interactions of MM cells with the osteoblasts via adhesion molecule, N-cadherin, in vitro. 5T33MM cells and 5TGM1 cells were cultured separately in vitro as described in methods. Mouse primary osteoblasts were obtained from calvarial bones of 2-4 day old C57Bl/6J mice, (Harlan, UK) and cultured in vitro as described in methods. Osteoblasts were differentiated into mature functional osteoblasts using an in vitro model of
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