This is accomplished by preincubating Anti-HIV-1 antibody the patient specimen with human? Anti-HIV-1 antibody is used for what P24 assay? If p24 antigen is present, the neutralizing Solid support antibody will form immune complexes and will prevent the antigen from binding to the HIV antibody on the? Absorbance should decrease by at least Positive test 50 percent on this test? In immune complexes, as the host P24 produces antibody, what antigen becomes undetectable? Primary screening for HIV-1 infection, ELISA test for HIV antibody p24 cannot replace this test? Quantitative tests for HIV nucleic acid is Load tests also known as? Load predict response to? Antiretroviral therapy load testing is performed by? Nucleic acid amplification methods load assays are based on? Amplification methods Viral load assays increase the number or HIV RNA copies what copies in test sample to detectable levels? What have been developed to diagnose Several amplification methods have been developed, HIV? including the reverse transcriptase polymerase chain reaction (RT-PCR) and real-time PCR, which amplify complementary DNA generated from HIV RNA; the branched chain DNA assay (bDNA), which amplifies a labeled signal bound to the test plate; and nucleic acid sequence-based amplification (NASBA), which amplifies HIV RNA. How many of polymerase chain Two: the reverse-transcriptase polymerase reaction methods have been developed to reaction (RT-PCR) and real-time PCR. detect HIV nucleic acid? Why do technical cares must be taken Because this assay is susceptible to when performing the RT-PCR? contamination of test samples with amplicon, target DNA, or target RNA. What can be done to minimize Physically separate work areas should be designated for reagent preparation, sample preparation, amplification, and detection, and the work should flow in one direction only. More recently, real-time RT-PCR assays quantitate HIV RNA have been developed to do what? How amplicon are analyzed in these They are analyzed at the same time they are produced assay? (i.e., in real time).
This is accomplished by preincubating the patient specimen with human? Anti-HIV-1 antibody Anti-HIV-1 antibody is used for what assay? P24 If p24 antigen is present, the neutralizing antibody will form immune complexes and will prevent the antigen from binding to the HIV antibody on the? Solid support Absorbance should decrease by at least 50 percent on this test? Positive test In immune complexes, as the host produces antibody, what antigen becomes undetectable? P24 Primary screening for HIV-1 infection, p24 cannot replace this test? ELISA test for HIV antibody Quantitative tests for HIV nucleic acid is also known as? Load tests Load predict response to? Antiretroviral therapy load testing is performed by? Nucleic acid amplification methods load assays are based on? Amplification methods Viral load assays increase the number or what copies in test sample to detectable levels? HIV RNA copies What have been developed to diagnose HIV? Several amplification methods have been developed, including the reverse transcriptase polymerase chain reaction (RT-PCR) and real-time PCR, which amplify complementary DNA generated from HIV RNA; the branched chain DNA assay (bDNA), which amplifies a labeled signal bound to the test plate; and nucleic acid sequence-based amplification (NASBA), which amplifies HIV RNA. How many of polymerase chain reaction methods have been developed to detect HIV nucleic acid? Two: the reverse-transcriptase polymerase reaction (RT-PCR) and real-time PCR. Why do technical cares must be taken when performing the RT-PCR? Because this assay is susceptible to of test samples with amplicon, target DNA, or target RNA. What can be done to minimize Physically separate work areas should be designated for reagent preparation, sample preparation, amplification, and detection, and the work should flow in one direction only. More recently, real-time RT-PCR assays have been developed to do what? quantitate HIV RNA How amplicon are analyzed in these assay? They are analyzed at the same time they are produced (i.e., in real time). contamination
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