Figure S1. The genealogy of PA06609 (a.k.a. PAO9), as reconstructed from the literature. Figure S2. (a) Levels of pyoverdin and pyochelin produced by wild-type in the P. aeruginosa different environments explored in this study. Molecules were detected by excitation-emission assays of 100 µl aliquots of culture supernatant and expressed as relative luminescence units (RLU) divided by the number of colony-forming units (CFU) present in 100 µl of the original culture. P. aeruginosa Symbols denote experimental block. (b) Bacterial density (CFU per 100 µl) the different environments. Symbols denote experimental block. CAA: casamino acids medium, ASM: artificial sputum medium, EVPL: pig lung model, SWF: synthetic wound fluid, SCW: synthetic chronic ex vivo wound model. Figure S3. Growth of wild type (black), (red) and (blue) in 200 P. aeruginosa ∆pvdD ∆pvdD∆pchEF µl ASM over 18 hours. Optical density was read at 400 nm to minimise interference from pyoverdine absorbance in wild-type cultures. Lines show means of four replica cultures, shaded areas show ± one standard deviation.
Figure S1. The genealogy of PA06609 (a.k.a. PAO9), as reconstructed from the literature. Figure S2. (a) Levels of pyoverdin and pyochelin produced by wild-type P. aeruginosa in the different environments explored in this study. Molecules were detected by excitation-emission assays of 100 µl aliquots of culture supernatant and expressed as relative luminescence units (RLU) divided by the number of P. aeruginosa colony-forming units (CFU) present in 100 µl of the original culture. Symbols denote experimental block. (b) Bacterial density (CFU per 100 µl) the different environments. Symbols denote experimental block. CAA: casamino acids medium, ASM: artificial sputum medium, EVPL: ex vivo pig lung model, SWF: synthetic wound fluid, SCW: synthetic chronic wound model. Figure S3. Growth of wild type (black), ∆pvdD (red) and ∆pvdD∆pchEF (blue) P. aeruginosa in 200 µl ASM over 18 hours. Optical density was read at 400 nm to minimise interference from pyoverdine absorbance in wild-type cultures. Lines show means of four replica cultures, shaded areas show ± one standard deviation.
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