What is important in effector functions of FC fragment immunoglobulin molecules, which include opsonization and complement fixation? What are the remaining 2 identical fragments Fab fragments (fragment antigen-binding) called? Because precipitation would not occur if Fab One antigen-binding site and that 2 such fragments were allowed to react with antigen, fragments were present in an intact antibody what was gu100,essed that each fragment molecule represented? What does each Fab fragment consist of? One L chain and one-half of a H chain How is each Fab fragment held together? Disulfide bonding What was to cleave IgG at the Proteolytic enzyme pepsin side of the interchain disulfide bonds, yielding one single fragment with molecular weight of 100,000 d and all the antigen-binding ability, known as F(ab)2? What additional fragment was similar to FC FC’ except that it disintegrated into several smaller pieces? What is found in the urine of patients with Bence Jones porteins multiple myeloma? What type of chains were being secreted by L chains malignant cells in patients with multiple myeloma? Who discovered Bence Jones proteins in 1845? Dr. Henry Bence Jones When heated to 60°C, Bence Jones proteins precipitated; redissolved ____ from urine, but on further heating to 80°C, they ____. What were the two main types of L chains in Kappa (κ) and lambda (λ) Bence Jones proteins? How much amino acid is contained in each L Between 200-220 amino acids chain? What was the region called from positions Constant region number 111 and one each type had essentially the same sequence? What was the amino-terminal end called? Variable region What is the difference between the and Amino acid substitutions at a few locations κ λ chains? along the chain (T or F) Both and L chains are found in all 5 True κ λ classes of immunoglobulins, but only one type is present in a given molecule. What demonstrated the presence of domains H chain sequencing similar to those in the L chains- that is, variable and constant regions? What is the first approximately 110 amino acids Variable domain at the amino-terminal end?
What is important in effector functions of immunoglobulin molecules, which include opsonization and complement fixation? FC fragment What are the remaining 2 identical fragments called? Fab fragments (fragment antigen-binding) Because precipitation would not occur if Fab fragments were allowed to react with antigen, what was gu100,essed that each fragment represented? One antigen-binding site and that 2 such fragments were present in an intact antibody molecule What does each Fab fragment consist of? One L chain and one-half of a H chain How is each Fab fragment held together? Disulfide bonding What was to cleave IgG at the side of the interchain disulfide bonds, yielding one single fragment with molecular weight of 100,000 d and all the antigen-binding ability, known as F(ab)2? Proteolytic enzyme pepsin What additional fragment was similar to FC except that it disintegrated into several smaller pieces? FC’ What is found in the urine of patients with multiple myeloma? Bence Jones porteins What type of chains were being secreted by malignant cells in patients with multiple myeloma? L chains Who discovered Bence Jones proteins in 1845? Dr. Henry Bence Jones When heated to 60°C, Bence Jones proteins ____ from urine, but on further heating to 80°C, they ____. precipitated; redissolved What were the two main types of L chains in Bence Jones proteins? Kappa (κ) and lambda (λ) How much amino acid is contained in each L chain? Between 200-220 amino acids What was the region called from positions number 111 and one each type had essentially the same sequence? Constant region What was the amino-terminal end called? Variable region What is the difference between the κ and λ chains? Amino acid substitutions at a few locations along the chain (T or F) Both κ and λ L chains are found in all 5 classes of immunoglobulins, but only one type is present in a given molecule. True What demonstrated the presence of domains similar to those in the L chains- that is, variable and constant regions? H chain sequencing What is the first approximately 110 amino acids at the amino-terminal end? Variable domain
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