to produce breakdown products that may be chromogenic, fluorogenic or luminescent? The following are examples of what? Typical enzymes used in labeled immnoassays Horseradish peroxidase, phosphate dehydrogenase, alkaline phosphatase, and β-D-galactosidase. Why are alkaline phosphatase and They have the highest turnover (conversion of horseradish peroxidase so often used in substrate), high sensitivity and are easy to detect labeled immunoassays? Enzyme assays are classified as either Heterogeneous or homogeneous ____ or _____? Enzyme assays are classified as Whether a separation step is necessary heterogeneous or homogeneous on the basis of what? What type of enzymatic immunoassay Heterogeneous enzyme immunoassays requires a step to physically separate free from bound analyte? What type of enzymatic immunoassay Homogeneous enzyme immunoassays requires no separation step? Why do homogenous enzyme Because enzyme activity diminishes when binding of immunoassays not require a separation antibody and antigen occurs step? The enzyme immunoassays (EIAs) RIA (radioimmunoassay) were competitive assays based on the principle of what type of assay? Enzyme activity is inversely proportional The more patient antigen is the less to the concentration of test substance. antigen can attach What does this mean? Currently type of immunoassays are Non-competitive enzyme immunoassays more common? Competitive or enzyme immunoassays? Although competitive have high Non-competitive enzyme immunoassays high specificity, why are non-competitive sensitivity and specificity, simplicity and low cost. enzyme immunoassays more common? Non-competitive assays are often ELISA (indirect enzyme-linked immunosorbent referred to as what? assays) In labeled immunoassays, either antigen Microtiter plates, nitrocellulose membranes, and or antibody may be bound to solid magnetic latex beads phases such as? When antigen is bound to solid phase, Unknown antibody is added to it and it is incubated what is done with patient serum? In labeled immunoassays, after a wash An enzyme-labeled antiglobulin. step, what is added?
to produce breakdown products that may be chromogenic, fluorogenic or luminescent? The following are examples of what? Horseradish peroxidase, dehydrogenase, alkaline phosphatase, and β-D-galactosidase. Typical enzymes used in labeled immnoassays Why are alkaline phosphatase and horseradish peroxidase so often used in labeled immunoassays? They have the highest turnover (conversion of substrate), high sensitivity and are easy to detect Enzyme assays are classified as either ____ or _____? Heterogeneous or homogeneous Enzyme assays are classified as heterogeneous or homogeneous on the basis of what? Whether a separation step is necessary What type of enzymatic immunoassay requires a step to physically separate free from bound analyte? Heterogeneous enzyme immunoassays What type of enzymatic immunoassay requires no separation step? Homogeneous enzyme immunoassays Why do homogenous enzyme immunoassays not require a separation step? Because enzyme activity diminishes when binding of antibody and antigen occurs The enzyme immunoassays (EIAs) were competitive assays based on the principle of what type of assay? RIA (radioimmunoassay) Enzyme activity is inversely proportional to the concentration of test substance. What does this mean? The more patient antigen is the less antigen can attach Currently type of immunoassays are more common? Competitive or enzyme immunoassays? Non-competitive enzyme immunoassays Although competitive have high specificity, why are non-competitive enzyme immunoassays more common? Non-competitive enzyme immunoassays high sensitivity and specificity, simplicity and low cost. Non-competitive assays are often referred to as what? ELISA (indirect enzyme-linked immunosorbent assays) In labeled immunoassays, either antigen or antibody may be bound to solid phases such as? Microtiter plates, nitrocellulose membranes, and magnetic latex beads When antigen is bound to solid phase, what is done with patient serum? Unknown antibody is added to it and it is incubated In labeled immunoassays, after a wash step, what is added? An enzyme-labeled antiglobulin. phosphate
Immuno%20PPT%20Flashcards%20Unit%202.docx