API 10S Enterobacteriaceae Salmonella Short & long rods on XLD + - API 20E API 10S Pseudomonas Short & long rods Large or fine, + D API 20NE (beaded) MacConkey positive. Microbact 12A & 12B Pseudomonas Diplococci Large or fine + D API 20NE Microbact 12A & 12B Pseudomonas Diplococcobacilli Large or fine + D API 20NE Microbact 12A & 12B Haemophilus, Pasteurella, Actinobacillus Pleomorphic Fine D D API 20NE Microbact 12A & 12B Fowl Pasteurella from chickens or API20NE (identification is Pleomorphic D D ostriches not listed, you must use own tables). Large and sometimes Fish bacteria (with salt Pleomorphic D + beta-haemolytic broth) Large or fine, growth at Fish bacteria (with salt Pleomorphic 25°C. Found in fish, D D broth) tortoises & snakes. Flat & spreading at Rods D D Myxobacteria 25°C. Found in fish Pleomorphic, bent Fine. Growth better with D D Campylobacter or spiral-shaped CO2 Table 2: Selection of biochemical tests for the Gram-positive bacteria, according to microscopic morphology, colony appearance, catalase and oxidase tests Colony Shape Colonies Cat. Oxi. Biochemical Morphology Short & long rods, Usually large + D Bacillus sometimes with spores Gram-positive Short & long rods Fine D D CoryneAPI Short & long rods, AnO2, - D Clostridium sometimes with spores
API 10S Short & long rods on XLD + - Enterobacteriaceae Salmonella API 20E API 10S Short & long rods (beaded) Large or fine, MacConkey positive. + D Pseudomonas API 20NE Microbact 12A & 12B Diplococci Large or fine + D Pseudomonas API 20NE Microbact 12A & 12B Diplococcobacilli Large or fine + D Pseudomonas API 20NE Microbact 12A & 12B Pleomorphic Fine D D Haemophilus, Pasteurella, Actinobacillus API 20NE Microbact 12A & 12B Pleomorphic from chickens or ostriches D D Fowl Pasteurella API20NE (identification is not listed, you must use own tables). Pleomorphic Large and sometimes beta-haemolytic D + Fish bacteria (with salt broth) Pleomorphic Large or fine, growth at 25°C. Found in fish, tortoises & snakes. D D Fish bacteria (with salt broth) Rods Flat & spreading at 25°C. Found in fish D D Myxobacteria Pleomorphic, bent or spiral-shaped Fine. Growth better with CO2 D D Campylobacter Table 2: Selection of biochemical tests for the Gram-positive bacteria, according to microscopic morphology, colony appearance, catalase and oxidase tests Shape Colony Morphology Colonies Cat. Oxi. Biochemical Short & long rods, sometimes with spores Usually large + D Bacillus Short & long rods Fine D D Gram-positive CoryneAPI Short & long rods, sometimes with spores AnO2, - D Clostridium
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