Zhang et al (2003) used immunohistochemistry technology to identify the HSC niche. It was found that LT-HSCs reside in the osteoblastic niche (Figure 1.5) and were only attached to the N-cadherin+ osteoblasts (). Xie et al (2009) developed new ex vivo imaging technology. Green fluorescent protein expressing (GFP+) HSCs homed through the vascular niche to the osteoblastic niche and attached to N-cadherin+ osteoblast cells in irradiated mice (). Lo Celso et al (2009) used a combination of high resolution confocal microscopy and two-photon video imaging technology that allowed observation of homing of individual HSC in calvarial bone marrow of living mice in real time. HSCs have been observed closer to bone and osteoblasts in irradiated and recipient mice (). It is only by using modern microscope that has
Zhang et al (2003) used immunohistochemistry technology to identify the HSC niche. It was found that LT-HSCs reside in the osteoblastic niche (Figure 1.5) and were only attached to the N-cadherin+ osteoblasts (). Xie et al (2009) developed new ex vivo imaging technology. Green fluorescent protein expressing (GFP+) HSCs homed through the vascular niche to the osteoblastic niche and attached to N-cadherin+ osteoblast cells in irradiated mice (). Lo Celso et al (2009) used a combination of high resolution confocal microscopy and two-photon video imaging technology that allowed observation of homing of individual HSC in calvarial bone marrow of living mice in real time. HSCs have been observed closer to bone and osteoblasts in irradiated and recipient mice (). It is only by using modern microscope that has
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