Galactose Sucrose Lactose Maltose Adonitol Dulcitol Sorbitol Salicin Table 5: Identification of some Gram-negative bacteria Campylobacter Fish bacteria Myxobacteria AnO2 BTA Gershman’s Maltose At 25°C: 0% Salt broth BTA in Campylobacter gas 0% Sout broth Sucrose 2% Salt broth BTA in candlejar @ 42°C 5% Salt broth Mannitol Gelatin BTA in candle jar at 25°C. 7% Salt broth Trehalose Aesculin TSI 3 ml MRP broth Glycerol Glucose Sodium hippurate Methylred Sorbitol O & F without oil Nitrate Citrate Salicin Casein agar Bruc. glucose & H2S Arginine 5% BTA DNAse agar Selenite Lysine 37°C BTA-ONPG Nitrate Nutrient broth Ornithine 0129 BTA disc Bruc. gluc. & H2S 25°C for motility Urea Starch Citrate Gelatine Vibrio agar Gershman’s Glucose Starch Arabinose AnO2 @ 25°C AnO2 Cytophaga agar Eggyolk Phosphatase agar Table 6: Biochemical tests recommended for the isolation of Gram-positive bacteria Actinomyces/ Gram-positive Staphylococcus Streptococcus Bacillus Corynebacterium O & F (x2) Nitrate Lactose Egg yolk Xylose Nitrate Glucose Mannitol Anaerobic BTA Galactose Glucose Maltose Raffinose Citrate Mannose Urea O & F Salicin 3ml MRP Lactose Aesculin DNAse Sorbitol Nitrate Sucrose Horse serum Trehalose Glucose Trehalose Brucella glucose & For identification of Inulin Horse serum Raffinose H2S coagulase negative Aesculin Salicin staphs: Sodium Hippurate Methylene blue Phosphatase agar
Galactose Sucrose Lactose Maltose Adonitol Dulcitol Sorbitol Salicin Table 5: Identification of some Gram-negative bacteria Campylobacter Fish bacteria Myxobacteria AnO2 BTA BTA in Campylobacter gas BTA in candlejar @ 42°C BTA in candle jar at 25°C. TSI Sodium hippurate Nitrate Bruc. glucose & H2S Selenite Nutrient broth 25°C for motility Gershman’s 0% Sout broth 5% Salt broth 7% Salt broth 3 ml MRP broth Methylred Citrate Arginine Lysine Ornithine Urea Gelatine Glucose Arabinose Maltose Sucrose Mannitol Trehalose Glycerol Sorbitol Salicin 5% BTA 37°C BTA-ONPG 0129 BTA disc Starch Vibrio agar At 25°C: 0% Salt broth 2% Salt broth Gelatin Aesculin Glucose O & F without oil Casein agar DNAse agar Nitrate Bruc. gluc. & H2S Citrate Gershman’s Starch AnO2 @ 25°C AnO2 Cytophaga agar Eggyolk Phosphatase agar Table 6: Biochemical tests recommended for the isolation of Gram-positive bacteria Gram-positive Staphylococcus Streptococcus Bacillus Actinomyces/ Corynebacterium O & F (x2) Nitrate Glucose Urea Aesculin Horse serum Brucella glucose & H2S Sodium Hippurate Nitrate Glucose Maltose O & F DNAse For identification of coagulase negative staphs: Lactose Mannitol Raffinose Salicin Sorbitol Trehalose Inulin Aesculin Methylene blue Egg yolk Anaerobic BTA Citrate 3ml MRP Nitrate Glucose Horse serum Xylose Galactose Mannose Lactose Sucrose Trehalose Raffinose Salicin Phosphatase agar
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