Immuno-localization of N-cadherin protein in myeloma cell lines, 5T33MM and 5TGM1 A rabbit monoclonal anti-mouse-N-cadherin antibody (Millipore, UK) and an isotype control, normal rabbit IgG antibody (R&D systems, UK) were used to determine possible localization of N-cadherin in 5T33MM and 5TGM1 myeloma cells. Northern light anti-rabbit IgG (NL637) antibody, (secondary antibody for immunofluorescence) or biotinylated anti-rabbit IgG antibody, (secondary antibody for immunocytochemistry) was used for detection of primary antibody. Figure 3.27 panel A shows positive staining (red fluorescence) of N-cadherin in 5T33MM cells and no staining with isotype control (Figure 3.27 panel B). Data determined that the
Immuno-localization of N-cadherin protein in myeloma cell lines, 5T33MM and 5TGM1 A rabbit monoclonal anti-mouse-N-cadherin antibody (Millipore, UK) and an isotype control, normal rabbit IgG antibody (R&D systems, UK) were used to determine possible localization of N-cadherin in 5T33MM and 5TGM1 myeloma cells. Northern light anti-rabbit IgG (NL637) antibody, (secondary antibody for immunofluorescence) or biotinylated anti-rabbit IgG antibody, (secondary antibody for immunocytochemistry) was used for detection of primary antibody. Figure 3.27 panel A shows positive staining (red fluorescence) of N-cadherin in 5T33MM cells and no staining with isotype control (Figure 3.27 panel B). Data determined that the
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