product ion, [M+11H]10+., formed from the [M+11H]11+ precursor ion after exposure to radical anions; (h) CCS of [M+12H]12+ precursor ion unexposed; (i) [M+12H]12+ precursor ion exposed; (j) CCS of the second product ion, [M+12H]10+.. formed from the [M+12H]12+ precursor ion after exposure to radical anions. Figure 7 Illustration of the neutralising contacts hypothesis used to describe the conformational change when an electron is transferred to a protein without the occurrence of dissociation (ETnoD). A reaction which occurs in the trap region of a Synapt G2 Si travelling wave ion mobility mass spectrometer. (a) The transfer of an electron disrupts a neutralising contact, a salt bridge between groups of opposite polarity, resulting in extension of the protein backbone chain which stays extended due to electrostatic repulsion. (b) The transfer of an electron disrupts a neutralising contact in a compact structure. Structural rearrangement in the form of compaction can occur, post electron transfer, if the free charged group pairs with an oppositely charge group in the surrounding area. 1 (Å2) of the most compact conformer before exposure and most extended conformer after TW exposure to radical anions of 1,3-dicyanobenzene. Cytochrome c and myoglobin were sprayed from denaturing solvent conditions (50/50 methano/water 0.1% formic acid).
product ion, [M+11H]10+., formed from the [M+11H]11+ precursor ion after exposure to radical anions; (h) CCS of [M+12H]12+ precursor ion unexposed; (i) [M+12H]12+ precursor ion exposed; (j) CCS of the second product ion, [M+12H]10+.. formed from the [M+12H]12+ precursor ion after exposure to radical anions. Figure 7 Illustration of the neutralising contacts hypothesis used to describe the conformational change when an electron is transferred to a protein without the occurrence of dissociation (ETnoD). A reaction which occurs in the trap region of a Synapt G2 Si travelling wave ion mobility mass spectrometer. (a) The transfer of an electron disrupts a neutralising contact, a salt bridge between groups of opposite polarity, resulting in extension of the protein backbone chain which stays extended due to electrostatic repulsion. (b) The transfer of an electron disrupts a neutralising contact in a compact structure. Structural rearrangement in the form of compaction can occur, post electron transfer, if the free charged group pairs with an oppositely charge group in the surrounding area. 1 TW (Å2) of the most compact conformer before exposure and most extended conformer after exposure to radical anions of 1,3-dicyanobenzene. Cytochrome c and myoglobin were sprayed from denaturing solvent conditions (50/50 methano/water 0.1% formic acid).
Jhingree_ETnoD_IJMS_resub.docx