What happens with active site on the the active site on the enzyme is blocked enzyme, when antibody binds to specific determinant sites on the antigen, resulting in a measurable loss of activity? Enzyme activity is directly in proportion concentration of patient antigen to what? Enzyme activity is directly in proportion hapten present in the test solution to what? Why sensitivity of homogeneous because the amplification properties of enzymes are immunoassays is far less than that not utilized achievable by heterogeneous enzyme assays? Enzyme immunoassays have achieved a RIA sensitivity similar to what assay without attendant health hazards or waste disposal problems? In what assays there is no need for Enzyme immunoassays expensive instrumentation, and reagents are inexpensive and have a long shelf life? What is disadvantage of enzyme some specimens may contain natural inhibitors immunoassays? The size of the enzyme label may be a Enzyme immunoassays limiting factor in the design of what assays? Nonspecific protein binding is potential Enzyme immunoassays difficulty in what assays? In what immunoassay fluorophores or fluorescent immunoassay techniques fluorochromes absorb energy from an incident light source and emit light of a longer wavelength and lower energy as the excited electrons return to the ground state? What two compounds most often used in fluorescent immunoassay techniques fluorescent immunoassay? Fluorescein and rhodamine are usually isothiocyanates used in what form? Why fluorescein and rhodamine are because these can be readily coupled with antigen or usually used in the form of antibody isothiocyanates? Why fluorescein and rhodamine are used Because their absorbance and emission patterns together? differ What compound can be used in phycoerythrin fluorescent immunoassay? What compound can be used in europium (β-naphthyl trifluoroacetone) fluorescent immunoassay?
What happens with active site on the enzyme, when antibody binds to specific determinant sites on the antigen, resulting in a measurable loss of activity? the active site on the enzyme is blocked Enzyme activity is directly in proportion to what? concentration of patient antigen Enzyme activity is directly in proportion to what? hapten present in the test solution Why sensitivity of homogeneous immunoassays is far less than that achievable by heterogeneous enzyme assays? because the amplification properties of enzymes are not utilized Enzyme immunoassays have achieved a sensitivity similar to what assay without attendant health hazards or waste disposal problems? RIA In what assays there is no need for expensive instrumentation, and reagents are inexpensive and have a long shelf life? Enzyme immunoassays What is disadvantage of enzyme immunoassays? some specimens may contain natural inhibitors The size of the enzyme label may be a limiting factor in the design of what assays? Enzyme immunoassays Nonspecific protein binding is potential difficulty in what assays? Enzyme immunoassays In what immunoassay fluorophores or fluorochromes absorb energy from an incident light source and emit light of a longer wavelength and lower energy as the excited electrons return to the ground state? fluorescent immunoassay techniques What two compounds most often used in fluorescent immunoassay? fluorescent immunoassay techniques Fluorescein and rhodamine are usually used in what form? isothiocyanates Why fluorescein and rhodamine are usually used in the form of isothiocyanates? because these can be readily coupled with antigen or antibody Why fluorescein and rhodamine are used together? Because their absorbance and emission patterns differ What compound can be used in fluorescent immunoassay? phycoerythrin What compound can be used in fluorescent immunoassay? europium (β-naphthyl trifluoroacetone)
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