of diabetes. The concentration of hemoglobin A1 is 3% to 6% in normal persons and 6% to 12% in both insulin-dependent and dependent diabetics. List the variant forms of normal hemoglobin: Carboxyhemoglobin Sulfhemoglobin Methemoglobin Where are abnormal hemoglobin molecules Abnormal hemoglobin found? molecules such as those seen in sickle cell anemia result from mutant, codominant genes. What method is used for identification of A method for the preliminary abnormal hemoglobin? identification of abnormal hemoglobin is electrophoresis. How was hemoglobin separated on In this method, hemoglobins electrophoresis? are separated on the basis of a complex interaction between hemoglobin, agar, and citrate buffer ions. How was hemoglobin migrated on Citrate agar Citrate agar separates electrophoresis? hemoglobin fractions that migrate together on cellulose acetatehemoglobins S, D, G, C, E, and O. When should hemoglobin specimens undergo All hemoglobin specimens electrophoresis on acid citrate agar? that show an abnormal electrophoretic pattern in alkaline media should undergo electrophoresis on acid citrate agar. What is the Kleihauer-Betke procedure? used to determine the It’s commonly amount of fetal blood that has mixed with maternal blood following delivery. How can chromatography be used in the analysis Quantitation of hemoglobin A1 can be of hemoglobin? accomplished by cation exchange minicolumn chromatography. However, the results of this technique can be affected by several types of hemoglobin in addition to hemoglobin A1. Cellulose acetate and citrate agar electrophoresis should be used in conjunction with cation exchange chromatography to eliminate the possibility of interference by hemoglobin variants. Other assay methods for glycosylated hemoglobin include high-pressure liquid
of diabetes. The concentration of hemoglobin A1 is 3% to 6% in normal persons and 6% to 12% in both insulin-dependent and diabetics. List the variant forms of normal hemoglobin: Carboxyhemoglobin Sulfhemoglobin Methemoglobin Where are abnormal hemoglobin molecules found? Abnormal hemoglobin molecules such as those seen in sickle cell anemia result from mutant, codominant genes. What method is used for identification of abnormal hemoglobin? A method for the preliminary identification of abnormal hemoglobin is electrophoresis. How was hemoglobin separated on electrophoresis? In this method, hemoglobins are separated on the basis of a complex interaction between hemoglobin, agar, and citrate buffer ions. How was hemoglobin migrated on Citrate agar electrophoresis? Citrate agar separates hemoglobin fractions that migrate together on cellulose acetatehemoglobins S, D, G, C, E, and O. When should hemoglobin specimens undergo electrophoresis on acid citrate agar? All hemoglobin specimens that show an abnormal electrophoretic pattern in alkaline media should undergo electrophoresis on acid citrate agar. What is the Kleihauer-Betke procedure? It’s commonly used to determine the amount of fetal blood that has mixed with maternal blood following delivery. How can chromatography be used in the analysis of hemoglobin? Quantitation of hemoglobin A1 can be accomplished by cation exchange minicolumn chromatography. However, the results of this technique can be affected by several types of hemoglobin in addition to hemoglobin A1. Cellulose acetate and citrate agar electrophoresis should be used in conjunction with cation exchange chromatography to eliminate the possibility of interference by hemoglobin variants. Other assay methods for glycosylated hemoglobin include high-pressure liquid dependent
Hematology%20PPT%20Flashcards%20Unit%202.docx