1. MODIFICATIONS TO PROCEDURES OR EXPERIMENTS (List each procedure or experiment to repeat, modify or add as new. Below the table, describe in detail how each of the proposed changes the original protocol) Change in animal numbers? Provide justification for proposed Type of change (complete below Procedure or experiment change (repeat, modify, new) table) [Type detailed description for each item listed the above table. Please provide a timeline of the additional procedures to reflect how and where they will be included in the project. For surgical procedures, submit Addendum 1 Surgical Procedure Form.] 2. MODIFICATIONS TO ANIMAL TYPE OR NUMBER (Changing strains or requesting additional animals) Check if M=male any are Total # F=female age genetically # animals # animals # animals Animals Species/Strains M/F=both range modified Category C Category D Category E* (C+D+E) *If adding new procedure or experiment under Category E (no pain/distress relief provided), provide justification. 3. MODIFICATIONS TO DRUG OR RESEARCH MATERIAL USED (List any the use of any new drugs or changes in the type or administration of drugs or research materials to be used in live animals) Species Drug/Agent Dose Route Frequency and When given? Pharma (0.00 mg/kg) (SQ, IV, (Pre, during or post op; duration of grade? IP, IM, prior to euthanasia, administration Yes N/A inhalation) treatment) (e.g., every 4 hrs. for 2 days, once) Provide justification for the change in drugs(s). If using anesthesia, provide at least two methods of monitoring the appropriate depth of anesthesia. Provide justification if not using pharmaceutic grade drugs or if using a paralytic.
1. MODIFICATIONS TO PROCEDURES OR EXPERIMENTS (List each procedure or experiment to repeat, modify or add as new. Below the table, describe in detail how each of the proposed changes the original protocol) Procedure or experiment Type of change (repeat, modify, new) Provide justification for proposed change Change in animal numbers? (complete below table) [Type detailed description for each item listed the above table. Please provide a timeline of the additional procedures to reflect how and where they will be included in the project. For surgical procedures, submit Addendum 1 Surgical Procedure Form.] 2. MODIFICATIONS TO ANIMAL TYPE OR NUMBER (Changing strains or requesting additional animals) Species/Strains Check if any are genetically modified M=male F=female M/F=both age range # animals Category C # animals Category D # animals Category E* Total # Animals (C+D+E) *If adding new procedure or experiment under Category E (no pain/distress relief provided), provide justification. 3. MODIFICATIONS TO DRUG OR RESEARCH MATERIAL USED (List any the use of any new drugs or changes in the type or administration of drugs or research materials to be used in live animals) Species Drug/Agent Dose (0.00 mg/kg) Route (SQ, IV, IP, IM, inhalation) Frequency and duration of administration (e.g., every 4 hrs. for 2 days, once) When given? (Pre, during or post op; prior to euthanasia, treatment) Pharma grade? Yes N/A Provide justification for the change in drugs(s). If using anesthesia, provide at least two methods of monitoring the appropriate depth of anesthesia. Provide justification if not using pharmaceutic grade drugs or if using a paralytic.
IACUC_Amendment_1Nov2018Final.docx