Dispense 15 of whole blood to the centre of Immediately following the addition of the μl the SAMPLE + BUFFER Well 1 of the device sample, (see Figure 2 below). use the Buffer dropper to add 2 drops (60 μl) of For venous whole blood use a laboratory Buffer, into the SAMPLE + BUFFER Well 1 pipette. For Fingerstick whole blood, follow the (see protocol Figure 3 below). using the Microtube 15 of the kit (see step 1 μl and 2 above). Figure 3 Figure 2 Wait 5-7 minutes The 6 blue coloured TEST lines should have disappeared from the Figure 4 rectangular TEST area. If not, discard the test device and repeat the procedure with a new test device. NOTE: A slight bluish-greenish colour may remain on the membrane, but none of the actual coloured lines should be seen at this point. Use the Buffer dropper to add 5 drops (150 μl) of Buffer into BUFFER Well 2 (see Figure 4). Read the test results between 20 to 30 minutes after the addition of the Buffer to BUFFER Well 2 Do not read results after 30 minutes. Read results in a well-lit area. NOTE: Discard the used pipette tips, test device and any other test materials into a biohazard container.
Dispense 15 μl of whole blood to the centre of the SAMPLE + BUFFER Well 1 of the device (see Figure 2 below). For venous whole blood use a laboratory pipette. For Fingerstick whole blood, follow the protocol using the Microtube 15 μl of the kit (see step 1 and 2 above). Figure 2 Immediately following the addition of the sample, use the Buffer dropper to add 2 drops (60 μl) of Buffer, into the SAMPLE + BUFFER Well 1 (see Figure 3 below). Figure 3 Wait 5-7 minutes The 6 blue coloured TEST lines should have disappeared from the rectangular TEST area. If not, discard the test device and repeat the procedure with a new test device. NOTE: A slight bluish-greenish colour may remain on the membrane, but none of the actual coloured lines should be seen at this point. Use the Buffer dropper to add 5 drops (150 μl) of Buffer into BUFFER Well 2 (see Figure 4). Figure 4 Read the test results between 20 to 30 minutes after the addition of the Buffer to BUFFER Well 2 Do not read results after 30 minutes. Read results in a well-lit area. NOTE: Discard the used pipette tips, test device and any other test materials into a biohazard container.
PHL%20Geenius%20Procedure.docx