1. Comparison of selected hand hygiene efficacy test methods by key step or variable ASTM Chlorine Key Step or E2783 EN 1276 Equivalency ASTM E1174 ASTM E2755 ASTM E2946 ASTM E2011 EN 1499 EN 1500 Variable (former USDA (Time Kill) E2/E3 rating) In Vitro In Vitro In In Vivo In Vivo In Vivo In Vivo In Vivo In Vivo Purpose / vitro” vitro” vitro” vivo” vivo” activity vivo” vivo” vivo” hand vivo” “In “In “In “In “In “In “In “In “In designed to test hand product of hand hygiene activity of antiviral washes hand rubs Target – – efficacy of Application in hygiene activity of evaluation personnel hand food handler activity of ensure a ensure a halogen based Design product disinfectants (“healthcare rubs hand hygiene hand hygiene minimum minimum disinfectants evaluation and hand personnel formulations formulations performance and sanitizers hygiene hand wash”) standard standard products Test Any BSL 1 Ps. S. aureus ATCC Serratia Serratia E. coli ATCC Human E. coli K12 E. coli K12 Organism(s) or 2 aeruginosa 6538 marcescens marcescens ATCC Rotavirus, NCTC 10538 NCTC 10538 organisms; ATCC 15442, and E. coli 14756 Human S. typhi ATCC we could E. coli ATCC Rhinovirus 6539 S. aureus ATCC recommend 10536, Type 37, 6538, or 33591 a specific Feline S. aureus list that are calicivirus, ATCC 6538, highly food Human relevant Adenovirus hirae ATCC (e.g. e. Coli, Type 5 10541 listeria, salmonella, etc.)
1. Comparison of selected hand hygiene efficacy test methods by key step or variable Key Step or Variable ASTM E2783 (Time Kill) EN 1276 Chlorine Equivalency (former USDA E2/E3 rating) ASTM E1174 ASTM E2755 ASTM E2946 ASTM E2011 EN 1499 EN 1500 In Vitro In Vitro In In Vivo In Vivo In Vivo In Vivo In Vivo In Vivo Purpose / Target Application in Design “In vitro” hand hygiene product evaluation “In vitro” activity of disinfectants and hand hygiene products “In vitro” designed to test efficacy of halogen based disinfectants and sanitizers “In vivo” product evaluation (“healthcare personnel hand wash”) “In vivo” activity of hand hygiene personnel hand rubs “In vivo” activity of food handler hand hygiene formulations “In vivo” antiviral activity of hand hygiene formulations “In vivo” hand washes – ensure a minimum performance standard “In vivo” hand rubs – ensure a minimum standard Test Organism(s) Any BSL 1 or 2 organisms; we could recommend a specific list that are highly food relevant (e.g. e. Coli, listeria, salmonella, etc.) Ps. aeruginosa ATCC 15442, E. coli ATCC 10536, S. aureus ATCC 6538, hirae ATCC 10541 S. aureus ATCC 6538 S. typhi ATCC 6539 Serratia marcescens and E. coli Serratia marcescens ATCC 14756 S. aureus ATCC 6538, or 33591 E. coli ATCC Human Rotavirus, Human Rhinovirus Type 37, Feline calicivirus, Human Adenovirus Type 5 E. coli K12 NCTC 10538 E. coli K12 NCTC 10538
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