What repeated contact with antigen A specific lymphocyte pool continually increase? What explains the kinetics of the immune Clonal selection response? Who proposed a solution to the tissue of the Dryer and Bennett large number of genes required by the clonal selection theory? What did Dryer and Bennett suggest? The constant and variable portions of immunoglobulin chains are actually coded by separate genes could be a small number coding for the Constant; variable ____ region and a larger number coding for the ____ region. What happens within lymphocytes? Genetic rearrangements What controls synthesis of a particular More than one gene immunoglobulin, and through a random selection process these individual segments are joined to commit that lymphocyte to making antibody of a single specificity? (T or F) B cells are genetically preprogrammed True to synthesize very specific antibody. What antibodies are used for diagnostic testing Monoclonal antibodies and for therapeutic purposes? Why is the normal response to antigen A purified antigen has multiple epitopes that heterogeneous? stimulate a variety of B-cell clones In 1975, what did Georges Kohler and Cesar A technique to produce antibody arising from a Milstein discover? single B cell In Kohler and Milstein’s technique, what is A myeloma cell fused with an activated B cell? What is a myeloma cell? A cancerous plasma cell (T or F) Plasma cells normally produce True antibody. What kind of cell line is chosen for the Kohler A particular cell line that is not capable of and Milstein’s technique? producing antibody What enzyme is deficient in the cell line that is Hypoxanthine guanine phosphoribosyl not capable of producing antibody? transferase (HGPRT) What enzyme is incapable of synthesizing Hypoxanthine guanine phosphoribosyl nucleotides from hypoxanthine and thymidine, transferase which are needed for DNA synthesis? What cells are harvested after a mouse is Spleen cells immunized with a certain antigen? What cells are combined in the presence of Spleen and myeloma cells polyethylene glycol (PEG), a surfactant? What is produced when the PEG brings about Hybridoma fusion of plasma cells with myeloma cells?
What repeated contact with antigen continually increase? A specific lymphocyte pool What explains the kinetics of the immune response? Clonal selection Who proposed a solution to the tissue of the large number of genes required by the clonal selection theory? Dryer and Bennett What did Dryer and Bennett suggest? The constant and variable portions of immunoglobulin chains are actually coded by separate genes could be a small number coding for the ____ region and a larger number coding for the ____ region. Constant; variable What happens within lymphocytes? Genetic rearrangements What controls synthesis of a particular immunoglobulin, and through a random selection process these individual segments are joined to commit that lymphocyte to making antibody of a single specificity? More than one gene (T or F) B cells are genetically preprogrammed to synthesize very specific antibody. True What antibodies are used for diagnostic testing and for therapeutic purposes? Monoclonal antibodies Why is the normal response to antigen heterogeneous? A purified antigen has multiple epitopes that stimulate a variety of B-cell clones In 1975, what did Georges Kohler and Cesar Milstein discover? A technique to produce antibody arising from a single B cell In Kohler and Milstein’s technique, what is fused with an activated B cell? A myeloma cell What is a myeloma cell? A cancerous plasma cell (T or F) Plasma cells normally produce antibody. True What kind of cell line is chosen for the Kohler and Milstein’s technique? A particular cell line that is not capable of producing antibody What enzyme is deficient in the cell line that is not capable of producing antibody? Hypoxanthine guanine phosphoribosyl transferase (HGPRT) What enzyme is incapable of synthesizing nucleotides from hypoxanthine and thymidine, which are needed for DNA synthesis? Hypoxanthine guanine phosphoribosyl transferase What cells are harvested after a mouse is immunized with a certain antigen? Spleen cells What cells are combined in the presence of polyethylene glycol (PEG), a surfactant? Spleen and myeloma cells What is produced when the PEG brings about fusion of plasma cells with myeloma cells? Hybridoma
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