molecules (; ). The networking effect is seen to a greater extent at higher protein concentrations, but lower phytic acid concentrations (1 10 mM). When some sites on a – lysozyme molecule are occupied by phytate there will be free sites on another lysozyme molecule that can interact with one of the free phosphate groups on the phytate molecule, as shown in In this model phytate is able to act as a bridge between two (or figure 3.2. potentially more) protein molecules (; ). At certain concentrations that were dependent on the protein being used, protein isoelectric point (pI) and pH of the solution, long chains and clusters of protein can be formed where phytate is acting as a bridge networking the protein molecules. The chain and complex formation starts to break down as the phytic acid concentration is increased further. This is due to all the available sites that phytic acid can interact with becoming saturated by phytate molecules. Once this happens cross-linking cannot take place as all sites are occupied with phytic acid. It is at this point that when the complexes start to break down and protein solubility starts to increase again. This model has been supported by solubility experiments presented in this work.
molecules (; ). The networking effect is seen to a greater extent at higher protein concentrations, but lower phytic acid concentrations (1 – 10 mM). When some sites on a lysozyme molecule are occupied by phytate there will be free sites on another lysozyme molecule that can interact with one of the free phosphate groups on the phytate molecule, as shown in figure 3.2. In this model phytate is able to act as a bridge between two (or potentially more) protein molecules (; ). At certain concentrations that were dependent on the protein being used, protein isoelectric point (pI) and pH of the solution, long chains and clusters of protein can be formed where phytate is acting as a bridge networking the protein molecules. The chain and complex formation starts to break down as the phytic acid concentration is increased further. This is due to all the available sites that phytic acid can interact with becoming saturated by phytate molecules. Once this happens cross-linking cannot take place as all sites are occupied with phytic acid. It is at this point that when the complexes start to break down and protein solubility starts to increase again. This model has been supported by solubility experiments presented in this work.
FINAL%20THESIS%20PASSED.docx