extended acclimation period, EPA recommends that the fish not be used. Type of food: [frozen brine shrimp, EPA recommends that fish be fed frozen brine other] shrimp twice per day to promote active reproduction and maintain body condition. Source of food: [............................] Frequency of feeding: [............................] times/day Details on feeding: [Describe any other relevant information about the feeding regime, including the amount given. Provide additional information if feeding conditions differed between the acclimation, pre-exposure, and definitive test periods.] Table 3: Fish Selection and Pre-Exposure Performance. Parameter Value(s) Details or Remarks Guideline Recommendations Age at test initiation: [...] months EPA recommends reproductively mature (sexually dimorphic) fish, 4.5 - 6 months old. a subsample Mean weight of males at test initiation [...] g [Indicate whether the mean is based on a EPA recommends that of fish be weighed before the test to estimate the (if determined): subsample of male fish or based on all male mean weight for each sex. It is recommended fish selected for the test.]
extended acclimation period, EPA recommends that the fish not be used. Type of food: [frozen brine shrimp, other] EPA recommends that fish be fed frozen brine shrimp twice per day to promote active reproduction and maintain body condition. Source of food: [............................] Frequency of feeding: [............................] times/day Details on feeding: [Describe any other relevant information about the feeding regime, including the amount given. Provide additional information if feeding conditions differed between the acclimation, pre-exposure, and definitive test periods.] Table 3: Fish Selection and Pre-Exposure Performance. Parameter Value(s) Details or Remarks Guideline Recommendations Age at test initiation: [...] months EPA recommends reproductively mature (sexually dimorphic) fish, 4.5 - 6 months old. Mean weight of males at test initiation (if determined): [...] g [Indicate whether the mean is based on a subsample of male fish or based on all male fish selected for the test.] EPA recommends that a subsample of fish be weighed before the test to estimate the mean weight for each sex. It is recommended
1350-fish-short-term-template.docx