C.2.3 Chronic toxicity to fish Test Substance Notified chemical (44.7% purity) Method US EPA TSCA 40CFR 797-1600 (1987) Protocol For – – Conducting an Early Stage Toxicity Test with Fathead Minnow (Pimephales promelas) Species minnow) Pimephales promelas (fathead Exposure period 35 days Auxiliary solvent None Water hardness 24–56 mg CaCO3/L Analytical HPLC monitoring Remarks - Method No significant protocol deviations. A preliminary range finding test resulted in 0% survival after 13 days of exposure to 10 mg/L. The definitive test involved 35 days of continuous exposure, which included a 5 day hatching period and a 30 day period. Results Concentration (mg/L) Number of % Embryo % Larval embryos hatching survival at success termination Nominal Actual* Control Control 60 81 98 0.63 0.69 60 78 94 1.3 1.5 60 88 95 2.5 2.5 60 80 94 5.0 4.8 60 89 93 10.0 10.0 60 0 0 * Mean measured concentrations LOEC 10 mg/L at 35 days NOEC 4.8. mg/L at 35 days Remarks - Results At 10 mg/L of the test substance, no animals survived the hatching period. Those organisms exposed to concentrations of the test substance at a nominal concentration of 5 mg/L (measured 4.8 mg/L) and below had a survival rate of 93 to 95
C.2.3 Chronic toxicity to fish Test Substance Notified chemical (44.7% purity) Method US EPA TSCA – 40CFR 797-1600 (1987) – Protocol For Conducting an Early Stage Toxicity Test with Fathead Minnow (Pimephales promelas) Species Pimephales promelas (fathead minnow) Exposure period 35 days Auxiliary solvent None Water hardness 24–56 mg CaCO3/L Analytical monitoring HPLC Remarks - Method No significant protocol deviations. A preliminary range finding test resulted in 0% survival after 13 days of exposure to 10 mg/L. The definitive test involved 35 days of continuous exposure, which included a 5 day hatching period and a 30 day period. Results Concentration (mg/L) Number of embryos % Embryo hatching success % Larval survival at termination Nominal Actual* Control Control 60 81 98 0.63 0.69 60 78 94 1.3 1.5 60 88 95 2.5 2.5 60 80 94 5.0 4.8 60 89 93 10.0 10.0 60 0 0 * Mean measured concentrations LOEC 10 mg/L at 35 days NOEC 4.8. mg/L at 35 days Remarks - Results At 10 mg/L of the test substance, no animals survived the hatching period. Those organisms exposed to concentrations of the test substance at a nominal concentration of 5 mg/L (measured 4.8 mg/L) and below had a survival rate of 93 to 95
GlucitolSTD735S-FINAL-06-2017.docx