Appendix A Table S1: Primers and PCR fragments amplified/used in this work. promoter of gene XXX; PXXX, terminator of gene Y-GDNA, CEN.PK113-7D genomic DNA; sequence annealing to TXXX, XXX; template in primers is shown in red and italics; over-lap sequence for over-lap extension PCR and Gibson Assembly is underlined; restriction sites used in cloning are shown in bold. No. PCR fragment Over-lap Primer -> Sequence (5’ 3’) extension PCR fragment 1 PM4pRS423s EfmvaS- TEFM1 PCUP1- EfmvaS- from pPMVAu8 TEFM1 PPM8+EFM1tas PPM8+EfmvaSaa from PPM8+TEF1pas PCUP1 PPM8+CUP1pa-s PPM8+CUP1pa-a PPM8+MCSa 2 from PPM8+MCSs PCUP1 PCUP1-EfmvaE- TEBS1 PPM8+CUP1pss PPM8+CUP1ps-a PPM8+TEF1psa 3 PPM8+EfmvaEss EfmvaE- TEBS1 from pPMVAu8 PM4pRS423a 4 from PMsuGAL1-10ps PGAL1/10 Y-DNA PGAL2 PMsuGAL1-10pa from ACS2 PMsuACS2s DNA PMsuACS2a from PMsuGAL2ps DNA PMsuGAL2pa
Appendix A Table S1: Primers and PCR fragments amplified/used in this work. PXXX, promoter of gene XXX; TXXX, terminator of gene XXX; Y-GDNA, CEN.PK113-7D genomic DNA; sequence annealing to template in primers is shown in red and italics; over-lap sequence for over-lap extension PCR and Gibson Assembly is underlined; restriction sites used in cloning are shown in bold. No. PCR fragment Over-lap extension PCR fragment Primer Sequence (5’ -> 3’) 1 EfmvaS- TEFM1 from pPMVAu8 PCUP1- EfmvaS- TEFM1 PM4pRS423s PPM8+EFM1tas PPM8+EfmvaSaa PCUP1 from PPM8+TEF1pas PPM8+CUP1pa-s PPM8+CUP1pa-a PPM8+MCSa 2 PCUP1 from PCUP1-EfmvaE- TEBS1 PPM8+MCSs PPM8+CUP1pss PPM8+CUP1ps-a PPM8+TEF1psa 3 EfmvaE- TEBS1 from pPMVAu8 PPM8+EfmvaEss PM4pRS423a 4 PGAL1/10 from Y-DNA PMsuGAL1-10ps PMsuGAL1-10pa ACS2 from PMsuACS2s PMsuACS2a PGAL2 from PMsuGAL2ps PMsuGAL2pa DNA DNA
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