Choose an “environmental change” for the mouth. Suggestions include: mouthwash, 12 soda, food, or anything else that could be placed and moved around in the mouth. Have the test subject put the choice in his or her mouth, and swish around the 13 “environmental change” for 30 seconds. For example, a soda can be swished just like a mouthwash. After 30 seconds, spit the choice into the sink or trash. Immediately have the test subject swirl a cotton swab in his 14 or her mouth for at least 5 seconds. On side B of the “Mouth” plate, starting at the middle, very 15 gently brush the cotton swab back and forth in a zig-zag motion (see image). Remember not to break the agar. Remove and discard the cotton swab. Place the lid back on the plate, and set the plate aside. 16 17 Choose one individual in your group to be the test subject for the “Skin” activity. Whenever removing the lid from the plate, it is important to not remove the lid 18 completely! Hold the lid over the plate. Contaminants in the air can easily settle and grow in the dish if the lid is completely removed. Remember, the nutrient agar in the plate is similar in consistency to Jello, so you are trying 19 to avoid breaking the surface of the agar here as well. On side A of the “Skin” plate and starting at the middle, have the test subject very gently 20 brush an index finger back and forth in a zig-zag motion. Place the lid back on the plate. Choose an “environmental change” for the skin. Suggestions include: lotion, soap, 21 disinfectant, or anything else that could be used on the skin. Have the test subject put the choice on both hands, and wait for 3 minutes. For example, 22 if the choice is lotion, have the test subject apply lotion to the hands in the usual way. On side B, starting at the middle, have the test subject very gently brush the index finger 23 back and forth in a zig-zag motion. Place the lid back on the plate. Turn both plates in to the instructor. If an incubator is available, the bacteria and fungus 24 will have grown by the following day. If not, it will be 2-3 days before the plates will be ready for observation. Part B. Observing the Plates Task Response
12 Choose an “environmental change” for the mouth. Suggestions include: mouthwash, soda, food, or anything else that could be placed and moved around in the mouth. 13 Have the test subject put the choice in his or her mouth, and swish around the “environmental change” for 30 seconds. For example, a soda can be swished just like a mouthwash. After 30 seconds, spit the choice into the sink or trash. 14 Immediately have the test subject swirl a cotton swab in his or her mouth for at least 5 seconds. 15 On side B of the “Mouth” plate, starting at the middle, very gently brush the cotton swab back and forth in a zig-zag motion (see image). Remember not to break the agar. 16 Remove and discard the cotton swab. Place the lid back on the plate, and set the plate aside. 17 Choose one individual in your group to be the test subject for the “Skin” activity. 18 Whenever removing the lid from the plate, it is important to not remove the lid completely! Hold the lid over the plate. Contaminants in the air can easily settle and grow in the dish if the lid is completely removed. 19 Remember, the nutrient agar in the plate is similar in consistency to Jello, so you are trying to avoid breaking the surface of the agar here as well. 20 On side A of the “Skin” plate and starting at the middle, have the test subject very gently brush an index finger back and forth in a zig-zag motion. Place the lid back on the plate. 21 Choose an “environmental change” for the skin. Suggestions include: lotion, soap, disinfectant, or anything else that could be used on the skin. 22 Have the test subject put the choice on both hands, and wait for 3 minutes. For example, if the choice is lotion, have the test subject apply lotion to the hands in the usual way. 23 On side B, starting at the middle, have the test subject very gently brush the index finger back and forth in a zig-zag motion. Place the lid back on the plate. 24 Turn both plates in to the instructor. If an incubator is available, the bacteria and fungus will have grown by the following day. If not, it will be 2-3 days before the plates will be ready for observation. Part B. Observing the Plates Task Response
Haspi-ecosystems-Student.docx