Proline (Pro) Asparagine (Asp) Leucine (Leu) CUA Glutamic acid (Glu) Valine (Val) 2. One of you will play the role of the ribosome and the other one will act as the cytoplasm, which is the source of tRNA and amino acid molecules. For tRNA molecules to function in translation, each tRNA must first be attached to the correct amino acid that corresponds to the anti-codon in that particular tRNA. Cytoplasm: Use the above table to match each model tRNA molecule with the correct amino acid for that particular type of tRNA Note: Each model tRNA molecule only shows the three nucleotides of the anti-codon and the binding site for the amino acid. A real tRNA molecule has many, many more nucleotides in an RNA polymer that is folded in roughly the shape shown in the figure on page 6. Similarly, each mRNA has many more nucleotides than shown in your model. Modeling the Steps in Translation: Note: You will be modeling the actual sequence of steps used by the cell to carry out translation. You probably will be able to think of a faster way to make the protein, but you should follow the sequence of steps described below in order to learn how the cell actually makes proteins. 3. Ribosome: Place the mRNA on the line in the model ribosome, with the first three nucleotides of the mRNA in the position for "codon" and the second mRNA codon in the "next codon" position. Cytoplasm: Supply the tRNA that has the correct anti-codon to match the first codon in the mRNA. Ribosome: Place this tRNA with amino acid in position. Your model ribosome should look like: Use an arrow to indicate the anti-codon in the tRNA and use an * to indicate the amino acid.
Proline (Pro) Asparagine (Asp) Leucine (Leu) CUA Glutamic acid (Glu) Valine (Val) 2. One of you will play the role of the ribosome and the other one will act as the cytoplasm, which is the source of tRNA and amino acid molecules. For tRNA molecules to function in translation, each tRNA must first be attached to the correct amino acid that corresponds to the anti-codon in that particular tRNA. Cytoplasm: Use the above table to match each model tRNA molecule with the correct amino acid for that particular type of tRNA Note: Each model tRNA molecule only shows the three nucleotides of the anti-codon and the binding site for the amino acid. A real tRNA molecule has many, many more nucleotides in an RNA polymer that is folded in roughly the shape shown in the figure on page 6. Similarly, each mRNA has many more nucleotides than shown in your model. Modeling the Steps in Translation: Note: You will be modeling the actual sequence of steps used by the cell to carry out translation. You probably will be able to think of a faster way to make the protein, but you should follow the sequence of steps described below in order to learn how the cell actually makes proteins. 3. Ribosome: Place the mRNA on the line in the model ribosome, with the first three nucleotides of the mRNA in the position for "codon" and the second mRNA codon in the "next codon" position. Cytoplasm: Supply the tRNA that has the correct anti-codon to match the first codon in the mRNA. Ribosome: Place this tRNA with amino acid in position. Your model ribosome should look like: Use an arrow to indicate the anti-codon in the tRNA and use an * to indicate the amino acid.
From%20Genes%20to%20Protein%20Review%20(tim%202013).docx