Parameter Minimum Maximum Mean Measurement Interval Guideline Recommendations EPA recommends temperature 25±1oC; Temperature (°C) inter-replicate and inter-treatment differentials should not exceed 1oC. pH EPA recommends pH 6.5 to 9.0. EPA recommends dissolved oxygen (DO) >4.9 Dissolved oxygen (mg/L) mg/L (>60% air saturation) EPA recommends total alkalinity > 20 mg/L as Total alkalinity (mg/L as CaCO3) CaCO3. Hardness [specify units] EPA recommends that total organic carbon in Total organic carbon (mg/L) dilution water be < 2 mg/L. EPA recommends that unionized ammonia in Unionized ammonia (g/L) the dilution water be < 1 g/L. EPA recommends that residual chlorine in Residual chlorine (g/L) dilution water be < 10 g/L. General recommendations for frequency of measurements: EPA recommends that temperature, pH, and dissolved oxygen be Other [specify] measured in all test tanks at least weekly and that hardness and alkalinity be measured in controls and in one tank at the highest test
Parameter Minimum Maximum Mean Measurement Interval Guideline Recommendations Temperature (°C) EPA recommends temperature 25±1oC; inter-replicate and inter-treatment differentials should not exceed 1oC. pH EPA recommends pH 6.5 to 9.0. Dissolved oxygen (mg/L) EPA recommends dissolved oxygen (DO) >4.9 mg/L (>60% air saturation) Total alkalinity (mg/L as CaCO3) EPA recommends total alkalinity > 20 mg/L as CaCO3. Hardness [specify units] Total organic carbon (mg/L) EPA recommends that total organic carbon in dilution water be < 2 mg/L. Unionized ammonia (g/L) EPA recommends that unionized ammonia in the dilution water be < 1 g/L. Residual chlorine (g/L) EPA recommends that residual chlorine in dilution water be < 10 g/L. Other [specify] General recommendations for frequency of measurements: EPA recommends that temperature, pH, and dissolved oxygen be measured in all test tanks at least weekly and that hardness and alkalinity be measured in controls and in one tank at the highest test
1350-fish-short-term-template.docx