in 5%(v/v) acetic acid diluted in ddH2O. (GE Healthcare, Little Chalfont, Bucks, UK) Nitrocellulose membrane Blocking solutions: Milk-PBST 5% (w/v) Marvel Original Dried Skimmed Milk 0.01% (v/v) Tween 20 in 1x PBS 5% Albumin bovine fraction V (BSA) (Melford BSA-PBST Laboratories Ltd., Chelsworth, Ipswich, UK) 0.01% (v/v) Tween20 in 1x PBS Western Ezier Super Blocking Buffer for Phospho-Specific Antibody Purchased from GenDEPOT to specifically preserve phosphoprotein antigen during blotting (GenDEPOT, Barker, TX, US) Wash buffer 0.01% (v/v) Tween-20 in 1 x PBS Enhanced chemiluminescent substrate (ECL) (EZ-ECL Biological Industries, Israel) Table 2.1. Primary antibodies used for western blotting Primary antibodies used for western blotting in this study. Antibody name, specificity, species, dilution, and source are shown. Abbreviation CST Cell Signaling Technology. Beverly, MA, US Antibody Specificity Species/Type Diluent and Source dilution C-terminus of the Rabbit polyclonal 5% Santa Cruz IGF1Rβ (C-20) IGF1 receptor BSA/PBST β 1:50 (SCBT), Dallas, Texas, US
in 5%(v/v) acetic acid diluted in ddH2O. Nitrocellulose membrane (GE Healthcare, Little Chalfont, Bucks, UK) Blocking solutions: Milk-PBST 5% (w/v) Marvel Original Dried Skimmed Milk 0.01% (v/v) Tween 20 in 1x PBS BSA-PBST 5% Albumin bovine fraction V (BSA) (Melford Laboratories Ltd., Chelsworth, Ipswich, UK) 0.01% (v/v) Tween20 in 1x PBS Western Ezier Super Blocking Buffer for Phospho-Specific Antibody Purchased from GenDEPOT to specifically preserve phosphoprotein antigen during blotting (GenDEPOT, Barker, TX, US) Wash buffer 0.01% (v/v) Tween-20 in 1 x PBS Enhanced chemiluminescent substrate (ECL) (EZ-ECL Biological Industries, Israel) Table 2.1. Primary antibodies used for western blotting Primary antibodies used for western blotting in this study. Antibody name, specificity, species, dilution, and source are shown. Abbreviation CST Cell Signaling Technology. Beverly, MA, US Antibody Specificity Species/Type Diluent and dilution Source IGF1Rβ (C-20) C-terminus of the IGF1 receptor β Rabbit polyclonal 5% BSA/PBST 1:50 Santa Cruz (SCBT), Dallas, Texas, US
FINAL%20thesis%20version%20jan%202017.docx