Mounting media Fluoromount Aqueous mounting medium (Sigma F4680) Table 2.5. Primary antibodies used for immunocytochemistry. Antibody reactivity, dilution and source are shown. Antibody Specificity Species/Type Diluent Source and dilution C-terminus of the Rabbit 3% Santa Cruz IGF1Rβ (C-20) IGF receptor polyclonal BSA/PBST β 1:25 (SCBT), Dallas, Texas, US C-terminus of the Rabbit 3% Santa Cruz IRβ human insulin monoclonal BSA/PBST receptor 1:25 (SCBT), β Dallas, Texas, US Vimentin Vimentin Mouse 3% Abcam, monoclonal BSA/PBST Cambridge, 1:250 UK, Ab8978 Synthetic peptides Chicken 3% Millipore β3-Tubulin from polyclonal BSA/PBST Lot 2424655 β3-Tubulin 1:1000 GFAP Full length GFAP Rabbit 3% Abcam, polyclonal BSA/PBST Cambridge, 1:1000 UK, Ab 7260 ALDH1L1 C-terminus of Rabbit 3% Abcam, ALDH1L1 polyclonal BSA/PBST Cambridge, 1:100 UK, Ab 79727 Connexin 43 C-terminal Rabbit 3% Sigma (Cx43) segment of the polyclonal BSA/PBST C6219 cytoplasmic 1:100 061M4823 domain of Cx43 (amino acids with added lysine) CD44 CD44 Mouse 3% Dako, monoclonal BSA/PBST Copenhagen, 1:100 Denmark, Clone DF1485
Mounting media Fluoromount Aqueous mounting medium (Sigma F4680) Table 2.5. Primary antibodies used for immunocytochemistry. Antibody reactivity, dilution and source are shown. Antibody Specificity Species/Type Diluent and dilution Source IGF1Rβ (C-20) C-terminus of the IGF receptor β Rabbit polyclonal 3% BSA/PBST 1:25 Santa Cruz (SCBT), Dallas, Texas, US IRβ C-terminus of the human insulin receptor β Rabbit monoclonal 3% BSA/PBST 1:25 Santa Cruz (SCBT), Dallas, Texas, US Vimentin Vimentin Mouse monoclonal 3% BSA/PBST 1:250 Abcam, Cambridge, UK, Ab8978 β3-Tubulin Synthetic peptides from β3-Tubulin Chicken polyclonal 3% BSA/PBST 1:1000 Millipore Lot 2424655 GFAP Full length GFAP Rabbit polyclonal 3% BSA/PBST 1:1000 Abcam, Cambridge, UK, Ab 7260 ALDH1L1 C-terminus of ALDH1L1 Rabbit polyclonal 3% BSA/PBST 1:100 Abcam, Cambridge, UK, Ab 79727 Connexin 43 (Cx43) C-terminal segment of the cytoplasmic domain of Cx43 (amino acids with added lysine) Rabbit polyclonal 3% BSA/PBST 1:100 Sigma C6219 061M4823 CD44 CD44 Mouse monoclonal 3% BSA/PBST 1:100 Dako, Copenhagen, Denmark, Clone DF1485
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