NFL Mouse 3% Novus (NFL) monoclonal BSA/PBST Biologicals 1:250 NB 300-132 Table 2.6. Secondary antibodies for immunocytochemistry. Antibody reactivity, dilution and source are shown. Antibody Species/Type Diluent and Source dilution Alexa fluor 488 Goat anti rabbit 3% Life (labelled with IgG (H-L) BSA/PBST technologies, green fluorescent 1:1000 Grand Island, dye) NY, USA Alexa fluor 568 Goat anti mouse 3% Life (labelled with IgG (H-L) BSA/PBST technologies, orange-red 1:1000 Grand Island, fluorescent dye) NY, USA Alex fluor 488 Goat anti chicken 3% Life (labelled with IgG (H-L) BSA/PBST technologies, green fluorescent 1:1000 Grand Island, dye) NY, USA 2.1.8 CellRox CellROX® Green Reagent (C10444, Life technologies, Oregon, USA Ref C10444) 2.1.9 Lactate Assay Lactate Assay Kit, including lactate standards (KA0833, Abnova). 2.1.10 Complex I Assay Complex I enzyme activity microplate assay kit (Abcam, ab109721, Cambridge, UK)
(NFL) NFL Mouse monoclonal 3% BSA/PBST 1:250 Novus Biologicals NB 300-132 Table 2.6. Secondary antibodies for immunocytochemistry. Antibody reactivity, dilution and source are shown. Antibody Species/Type Diluent and dilution Source Alexa fluor 488 (labelled with green fluorescent dye) Goat anti rabbit IgG (H-L) 3% BSA/PBST 1:1000 Life technologies, Grand Island, NY, USA Alexa fluor 568 (labelled with orange-red fluorescent dye) Goat anti mouse IgG (H-L) 3% BSA/PBST 1:1000 Life technologies, Grand Island, NY, USA Alex fluor 488 (labelled with green fluorescent dye) Goat anti chicken IgG (H-L) 3% BSA/PBST 1:1000 Life technologies, Grand Island, NY, USA 2.1.8 CellRox CellROX® Green Reagent (C10444, Life technologies, Oregon, USA Ref C10444) 2.1.9 Lactate Assay Lactate Assay Kit, including lactate standards (KA0833, Abnova). 2.1.10 Complex I Assay Complex I enzyme activity microplate assay kit (Abcam, ab109721, Cambridge, UK)
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