Table 1. Colour reactions of a pure cercosporin standard and the crude pigment extracted from hyphae of a three-week-old culture of Pseudocercosporella capsellae in malt extract broth using standard reagents and procedures as used in the studies listed (see references below). Reagent Colour of the pigment extract Colour of the standard cercosporin Reference 1 M KOH (; ; ) Green Green 1 M HCl (; ; ) Red Red 1 M NaOH () Green Green Concentrated H2SO4 (; ; ) Purple Purple H2O (; ) Insoluble Insoluble Concentrated H2SO4 + H2O (; ; ) Bluish purple precipitate Bluish purple precipitate () Acetone Bright red Bright red () Acidic + neutral conditions (< 7.7 pH) Red Red Basic conditions (>7.7 pH) Green Green (; )
Table 1. Colour reactions of a pure cercosporin standard and the crude pigment extracted from hyphae of a three-week-old culture of Pseudocercosporella capsellae in malt extract broth using standard reagents and procedures as used in the studies listed (see references below). Reagent Colour of the pigment extract Colour of the standard cercosporin Reference 1 M KOH Green Green (; ; ) 1 M HCl Red Red (; ; ) 1 M NaOH Green Green () Concentrated H2SO4 Purple Purple (; ; ) H2O Insoluble Insoluble (; ) Concentrated H2SO4 + H2O Bluish purple precipitate Bluish purple precipitate (; ; ) Acetone Bright red Bright red () Acidic + neutral conditions (< 7.7 pH) Red Red () Basic conditions (>7.7 pH) Green Green (; )
Gunasinghe%20et%20al%20-%20PDIS-10-15-1192-RE%20-%20FIRST%20LOOK%20Manuscript%20.docx