In vitro Culture Collections Box 3.1.1.B Initial viability IV1 - Describe the procedures or practices that you have in place to ensure the highest possible initial viability of your plant material, in particular during culture of donor plants (e.g. cultivation practices [field, greenhouse], phytosanitary pre-treatments, like use of pesticides). IV2 Describe procedures of explant isolation (organ source in the plant, – manipulations) and sterilization (chemical and handling) of the explants. IV3 Please provide any other information on procedures that you follow to – ensure highest possible initial viability. Box 3.1.2 .B Viability Monitoring VM1 - Describe the routine in vitro viability monitoring system that you use. (The monitoring system should include the following aspects: regular control of contamination events, control of hyper-hydricity, control of health state (if different from a above), etc). VM2 - Describe the information (i.e. an that you “system” “expert system”) might have in place that allows you to make more species or even decisions when the next monitoring should take place. VM3 - Please provide information on non-specific thresholds that you might use for vigor of in vitro cultures (i. e. multiplication rates, loss by weak growth) and for the amount of culture vessels (tubes, jars) left of an accession to initiate additional multiplication measures? Box 3.1.3.B Storage Conditions (for the different types of collections i.e. short/medium- or long-term storage) SC1 - Please provide details on light, temperature and relative humidity conditions of your culture and storage rooms, as applicable. In case they vary from room to room, please provide details for each. SC2 Provide details on the type of cultivation vessels (tubes, jars plastic – vessels etc.) and the transfer procedures (including the corresponding equipment, if any) that you use. SC3 Please include any other aspects regarding in vitro culture and storage – conditions at your genebank that you regard as important.
In vitro Culture Collections Box 3.1.1.B Initial viability IV1 - Describe the procedures or practices that you have in place to ensure the highest possible initial viability of your plant material, in particular during culture of donor plants (e.g. cultivation practices [field, greenhouse], phytosanitary pre-treatments, like use of pesticides). IV2 – Describe procedures of explant isolation (organ source in the plant, manipulations) and sterilization (chemical and handling) of the explants. IV3 – Please provide any other information on procedures that you follow to ensure highest possible initial viability. Box 3.1.2 .B Viability Monitoring VM1 - Describe the routine in vitro viability monitoring system that you use. (The monitoring system should include the following aspects: regular control of contamination events, control of hyper-hydricity, control of health state (if different from a above), etc). VM2 - Describe the information “system” (i.e. an “expert system”) that you might have in place that allows you to make more species or even decisions when the next monitoring should take place. VM3 - Please provide information on non-specific thresholds that you might use for vigor of in vitro cultures (i. e. multiplication rates, loss by weak growth) and for the amount of culture vessels (tubes, jars) left of an accession to initiate additional multiplication measures? Box 3.1.3.B Storage Conditions (for the different types of collections i.e. short/medium- or long-term storage) SC1 - Please provide details on light, temperature and relative humidity conditions of your culture and storage rooms, as applicable. In case they vary from room to room, please provide details for each. SC2 – Provide details on the type of cultivation vessels (tubes, jars plastic vessels etc.) and the transfer procedures (including the corresponding equipment, if any) that you use. SC3 – Please include any other aspects regarding in vitro culture and storage conditions at your genebank that you regard as important.
FINAL_VERSION_template_for_operational_genebank_manuals__formally_approved_by_the_AEGIS_Advisory_Committee_of_24.09.2010_.docx