Include a graph for each run (2 or 3 runs) as depicted below in Figure 1. Relative (to the PC) transcriptional activation at each concentration of the test chemical during the [2 or 3] assay runs is presented in Table 5, along with the mean of [both or all] of the runs. The concentration-response curves depicting fold induction of relative transcriptional activation is presented in Figure 1 (Figure 2, and Figure 3 if applicable) below. The mean RPCmax was [#]%, and the associated PCmax was [#] M. Because the RPCMax PC10 in [both or two of the three] runs, [chemical] was considered positive for ≥ estrogen receptor transcriptional activation. The PC50 was [10-n] M. An example of a negative response may read as follows: Because the RPCMax < PC10 in [two of the three or all] runs, [test material] was considered negative for estrogen receptor transcriptional activation in this test system. Table 5. Relative Transcriptional Activation (RTA) of [chemical]a Parameter RTA (mean ± SD); % of Positive Control (PC) Run 1 Run 2 Run 3 Conc. (M) Mean SD Mean SD Mean SD [10-5] [10-6] [10-7] [10-8] [10-9] [10-10] [10-11] Log EC50b Hill Slopeb RPCmax PCmax PC50 PC10 a Data were obtained from page [#] of the study report. Include if 3 runs were conducted. Delete third column if only two runs were conducted. b Report if appropriate NA= Not Applicable Figure 1. Fold Induction of Relative Transcription Activation (RTA) of [chemical] compared to the Positive Control. VC= Vehicle Control PC= Positive Control (1 nM E2)
Include a graph for each run (2 or 3 runs) as depicted below in Figure 1. Relative (to the PC) transcriptional activation at each concentration of the test chemical during the [2 or 3] assay runs is presented in Table 5, along with the mean of [both or all] of the runs. The concentration-response curves depicting fold induction of relative transcriptional activation is presented in Figure 1 (Figure 2, and Figure 3 if applicable) below. The mean RPCmax was [#]%, and the associated PCmax was [#] M. Because the RPCMax ≥ PC10 in [both or two of the three] runs, [chemical] was considered positive for estrogen receptor transcriptional activation. The PC50 was [10-n] M. An example of a negative response may read as follows: Because the RPCMax < PC10 in [two of the three or all] runs, [test material] was considered negative for estrogen receptor transcriptional activation in this test system. Table 5. Relative Transcriptional Activation (RTA) of [chemical]a Parameter RTA (mean ± SD); % of Positive Control (PC) Run 1 Run 2 Run 3 Conc. (M) Mean SD Mean SD Mean SD [10-5] [10-6] [10-7] [10-8] [10-9] [10-10] [10-11] Log EC50b Hill Slopeb RPCmax PCmax PC50 PC10 a Data were obtained from page [#] of the study report. Include if 3 runs were conducted. Delete third column if only two runs were conducted. b Report if appropriate NA= Not Applicable Figure 1. Fold Induction of Relative Transcription Activation (RTA) of [chemical] compared to the Positive Control. VC= Vehicle Control PC= Positive Control (1 nM E2)
1300-erta-template.docx