APPENDIX A RECOMBINANT/SYNTHETIC NUCLEIC ACIDS – Briefly describe, in lay terms, the work to be performed with recombinant or synthetic nucleic acid molecules. List the vectors included in this project: Gene Transfer Method Vector Type Technical Name (transformation, transfection, (plasmid, viral, phage, etc.) (include vendor if applicable) electroporation, etc.) List the genes/nucleic acids included in this project: Nature of Insert or Gene/Nucleic Acid Source Organism Use/Purpose Protein Expressed (specific name of gene, (genus and species or (cloning for sequencing, PCR, promoter, regulatory (reporter gene, virulence common name) expression, etc.) sequence, etc.) factor, DNA repair, etc.) Attach and submit a detailed map of a representative sample of the vectors and inserts to be used. Indicate any regions that increase the safety of this construct. Provide copies of key references that describe the construction of the vector(s) used. List any proteins that are produced: Will the nucleic acids contain genes for the biosynthesis of toxic molecules lethal to vertebrates? No Yes (If yes, list the toxin’s LD50): Will the nucleic acids contain genetic material from ? No Yes:
APPENDIX A – RECOMBINANT/SYNTHETIC NUCLEIC ACIDS Briefly describe, in lay terms, the work to be performed with recombinant or synthetic nucleic acid molecules. List the vectors included in this project: Vector Type (plasmid, viral, phage, etc.) Technical Name (include vendor if applicable) Gene Transfer Method (transformation, transfection, electroporation, etc.) List the genes/nucleic acids included in this project: Gene/Nucleic Acid (specific name of gene, promoter, regulatory sequence, etc.) Source Organism (genus and species or common name) Nature of Insert or Protein Expressed (reporter gene, virulence factor, DNA repair, etc.) Use/Purpose (cloning for sequencing, PCR, expression, etc.) Attach and submit a detailed map of a representative sample of the vectors and inserts to be used. Indicate any regions that increase the safety of this construct. Provide copies of key references that describe the construction of the vector(s) used. List any proteins that are produced: Will the nucleic acids contain genes for the biosynthesis of toxic molecules lethal to vertebrates? No Yes (If yes, list the toxin’s LD50): Will the nucleic acids contain genetic material from ? No Yes:
Form%20-%20Appendix%20A%20Recombinant%20Synthetic%20Nucleic%20Acids.docx