Table S1. Mass spectrometric multiple reaction monitoring detection of protein glycation, oxidation and nitration adducts and amino acids (cont’d). Analyte Analyte Rt Parent ion Fragment ion CE Neutral fragment loss(es) Internal standard and amount group (min) (Da) (Da) (eV) added Oxidative MetSO 8.7 166.1 102.2 14 CH3-SOH [2H3]MetSO, 0.25 pmol DT 19.9 361.2 315.3 15 H2CO2 [2H6]DT, 0.25 pmol NFK 21.5 235.8 191.2 18 H2CO2 [15N2]NFK, 0.25 pmol AASA 10.7 128.0 82.0 15 H2CO2 [2H3]AAA (Rt = 29.4), 2.5 pmol GSA 32.2 114.0 68.0 15 H2CO2 [2H3]AAA (Rt = 29.4), 2.5 pmol Nitration 3-NT 23.2 227.1 181.2 13 H2CO2 [2H3]3-NT, 0.25 pmol Amino acids Ala 5.2 90.1 44.1 8 H2CO2 [2H3]Ala, 250 pmol Arg 29.2 175.2 70.3 15 H2CO2, NH2C(=NH)NH2 [15N2]Arg, 250 pmol Asn 7.2 133.2 74.1 14 CH3CONH2 [13C4]Asp, 250 pmol
Table S1. Mass spectrometric multiple reaction monitoring detection of protein glycation, oxidation and nitration adducts and amino acids (cont’d). Analyte group Analyte Rt (min) Parent ion (Da) Fragment ion (Da) CE (eV) Neutral fragment loss(es) Internal standard and amount added Oxidative MetSO 8.7 166.1 102.2 14 CH3-SOH [2H3]MetSO, 0.25 pmol DT 19.9 361.2 315.3 15 H2CO2 [2H6]DT, 0.25 pmol NFK 21.5 235.8 191.2 18 H2CO2 [15N2]NFK, 0.25 pmol AASA 10.7 128.0 82.0 15 H2CO2 [2H3]AAA (Rt = 29.4), 2.5 pmol GSA 32.2 114.0 68.0 15 H2CO2 [2H3]AAA (Rt = 29.4), 2.5 pmol Nitration 3-NT 23.2 227.1 181.2 13 H2CO2 [2H3]3-NT, 0.25 pmol Amino acids Ala 5.2 90.1 44.1 8 H2CO2 [2H3]Ala, 250 pmol Arg 29.2 175.2 70.3 15 H2CO2, NH2C(=NH)NH2 [15N2]Arg, 250 pmol Asn 7.2 133.2 74.1 14 CH3CONH2 [13C4]Asp, 250 pmol
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