Gel Electrophoresis Lab (DNA Fingerprinting) Notes From the teacher Day 1: Before class: Read the entire investigation. Complete the pre-lab for Part A and Part B: Title Purpose general purpose for the whole lab – PreLab Questions for BOTH Parts A and B copy the question and write the answer for each of the questions. In class: Discuss Parts A and B of the lab Explain what we will be doing for Part C tomorrow, and that we are just setting up the lab stations today Complete the following: Obtain 6 clear micro test tubes and label their lids as follows: CS crime suspect – S1 suspect 1 – S2 suspect 2 – S3 suspect 3 – S4 suspect 4 – S5 suspect 5 – Obtain the microtube floating rack. Label with your group names and block #. Get with your lab group and make sure you have all your necessary materials and practice loading the gels Mrs. Willis will show you how to do this! Day 2: Before class: Complete the Pre-lab Questions for Part C based on what we have learned and completed so far. Write the Procedure for Part C. Instead of writing every step that is covered in the Quick Guide, you may tape into your lab notebook. the Quick Guide In class: Obtain your micro test tubes that you labeled yesterday and an enzyme sample from the teacher. Make sure to keep the enzyme on ice. Work through Day 1 of the Quick Guide Preparing the DNA Samples When you are done, put your samples in the container for your block and they will be placed in the fridge overnight Day 3 Before class: N/A In class: Work through Gel Electrophoresis Day 2 of the Quick Guide Make sure to run your gel at 150V for approximately 40 minutes
Gel Electrophoresis Lab (DNA Fingerprinting) Notes From the teacher Day 1: Before class: Read the entire investigation. Complete the pre-lab for Part A and Part B: Title Purpose – general purpose for the whole lab PreLab Questions for BOTH Parts A and B copy the question and write the answer for each of the questions. In class: Discuss Parts A and B of the lab Explain what we will be doing for Part C tomorrow, and that we are just setting up the lab stations today Complete the following: Obtain 6 clear micro test tubes and label their lids as follows: CS – crime suspect S1 – suspect 1 S2 – suspect 2 S3 – suspect 3 S4 – suspect 4 S5 – suspect 5 Obtain the microtube floating rack. Label with your group names and block #. Get with your lab group and make sure you have all your necessary materials and practice loading the gels Mrs. Willis will show you how to do this! Day 2: Before class: Complete the Pre-lab Questions for Part C based on what we have learned and completed so far. Write the Procedure for Part C. Instead of writing every step that is covered in the Quick Guide, you may tape the Quick Guide into your lab notebook. In class: Obtain your micro test tubes that you labeled yesterday and an enzyme sample from the teacher. Make sure to keep the enzyme on ice. Work through Day 1 of the Quick Guide Preparing the DNA Samples When you are done, put your samples in the container for your block and they will be placed in the fridge overnight Day 3 Before class: N/A In class: Work through Day 2 of the Quick Guide Gel Electrophoresis Make sure to run your gel at 150V for approximately 40 minutes
GEL%20ELECTROPHORESIS%20LAB.docx