C-terminus of the Rabbit 5% CST IRβ human insulin monoclonal BSA/PBST receptor 1:1000 β IRS1 Amino acids Rabbit polyclonal Western CST of IRS-1 Ezier human protein. 1:1000 IRS2 IRS-2 human Rabbit polyclonal Western CST protein Ezier 1:1000 Phospho Akt Human Akt Rabbit Western CST XP (ser473) phosphorylated at monoclonal Ezier ser473 site. 1:1000 Akt Akt human protein Rabbit polyclonal Western CST Ezier 1:1000 Phospho-p44/42 MAPK human Rabbit polyclonal Western CST MAPK (Erk1/2) protein Ezier phosphorylated at 1:1000 ) residue Thr202/Tyr204 p44/42 MAPK MAPK human Rabbit polyclonal Western CST (Erk1/2) protein Ezier 1:1000 antibody human Mouse 5% Abcam, β-actin β-actin protein monoclonal Milk/PBST 1:10000 , UK Phosphopeptide Rabbit Western R&D ϒ-H2AX containing human polyclonal Ezier systems, Histone H2AX 1:1000 MN, USA S139 site GFAP GFAP Rabbit polyclonal Western Abcam, Ezier 1:1000 , UK Ab7260 Alpha-tubulin Synthetic peptide Rabbit polyclonal Western Abcam, conjugated to KLH Ezier derived from 1:5000 UK within residues 1 - Ab4074 100 of Human alpha Tubulin. Neurofilament NFL Mouse Western Novus Light (NFL) monoclonal Ezier 1:1000 s
IRβ C-terminus of the human insulin receptor β Rabbit monoclonal 5% BSA/PBST 1:1000 CST IRS1 Amino acids of IRS-1 human protein. Rabbit polyclonal Western Ezier 1:1000 CST IRS2 IRS-2 human protein Rabbit polyclonal Western Ezier 1:1000 CST Phospho Akt XP (ser473) Human Akt phosphorylated at ser473 site. Rabbit monoclonal Western Ezier 1:1000 CST Akt Akt human protein Rabbit polyclonal Western Ezier 1:1000 CST Phospho-p44/42 MAPK (Erk1/2) MAPK human protein phosphorylated at residue Thr202/Tyr204 Rabbit polyclonal Western Ezier 1:1000 CST p44/42 MAPK (Erk1/2) MAPK human protein Rabbit polyclonal Western Ezier 1:1000 CST β-actin antibody β-actin human protein Mouse monoclonal 5% Milk/PBST 1:10000 Abcam, UK ϒ-H2AX Phosphopeptide containing human Histone H2AX S139 site Rabbit polyclonal Western Ezier 1:1000 R&D systems, MN, USA GFAP GFAP Rabbit polyclonal Western Ezier 1:1000 Abcam, UK Ab7260 Alpha-tubulin Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human alpha Tubulin. Rabbit polyclonal Western Ezier 1:5000 Abcam, UK Ab4074 Neurofilament Light (NFL) NFL Mouse monoclonal Western Ezier 1:1000 Novus ) s , ,
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