GFP LUHMES were co-cultured with DMSO or Wortmannin pretreated astrocytes for 24, 48 and 72 hours. Representative images show GFP LUHMES co-cultured with DMSO and Wortmannin pretreated astrocytes. Bar charts represent the quantification of the neurite outgrowth at each time point. Data represents mean ± SEM (n=3, 3 replicates/experiment, 5 fields counted per coverslip (represents 1 replicate), one way ANOVA with post-hoc analysis **p<0.01 ****p<0.0001).
GFP LUHMES were co-cultured with DMSO or Wortmannin pretreated astrocytes for 24, 48 and 72 hours. Representative images show GFP LUHMES co-cultured with DMSO and Wortmannin pretreated astrocytes. Bar charts represent the quantification of the neurite outgrowth at each time point. Data represents mean ± SEM (n=3, 3 replicates/experiment, 5 fields counted per coverslip (represents 1 replicate), one way ANOVA with post-hoc analysis **p<0.01 ****p<0.0001).
FINAL%20thesis%20version%20jan%202017.docx