LAB TECHNIQUE ACTIVTY SHEET Name____________________________________________ Micropipette P-20 1. Use the display window in the following micropipettors to determine the volume they will collect/dispense. a. _______ b. _______ c. _______ d. _______ P-20, 2. If you were trying to measure the following volumes with a what would the display window show? a. b. 3.0 c. 12.0 d. 20.0 2.5 μL μL μL μL P-200, 3. If you were trying to measure the following volumes with a what would the display window show? a. 250 b. 30 c. 99 d. 100 μL μL μL μL P-1000, 4. If you were trying to measure the following volumes with a what would the display window show? a. 350 b. 270 c. 1000 d. 60 μL μL μL μL Reminder: How to Use Micropippete 1) set the volume 4) immerse tip in sample 8) dispense the sample 2) attach disposable tip 5) draw up the sample 9) withdraw the pipette 3) depress the plunger to the first 6) pause 10) release plunger stop 7) withdraw the tip 11) discard the tip
LAB TECHNIQUE ACTIVTY SHEET Name____________________________________________ Micropipette 1. Use the display window in the following P-20 micropipettors to determine the volume they will collect/dispense. a. _______ b. _______ c. _______ d. _______ 2. If you were trying to measure the following volumes with a P-20, what would the display window show? a. 2.5 μL b. 3.0 μL c. 12.0 μL d. 20.0 μL 3. If you were trying to measure the following volumes with a P-200, what would the display window show? a. 250 μL b. 30 μL c. 99 μL d. 100 μL 4. If you were trying to measure the following volumes with a P-1000, what would the display window show? a. 350 μL b. 270 μL c. 1000 μL d. 60 μL Reminder: How to Use Micropippete 1) set the volume 2) attach disposable tip 3) depress the plunger to the first stop 4) immerse tip in sample 5) draw up the sample 6) pause 7) withdraw the tip 8) dispense the sample 9) withdraw the pipette 10) release plunger 11) discard the tip
HANDOUT-Micropipettor-Centrifuge_Practice_18.docx