The effect of enzyme concentration on the rate of a reaction STUDENT Introduction Trypsin is a protease that catalyses the breakdown of other proteins. By making a serial dilution of Trypsin solution, you will observe the effect of changing enzyme concentration on the rate of a reaction. Aim To investigate the effect of changing the enzyme concentration on the rate of milk protein breakdown by Trypsin. Intended class time 1 hour Equipment Milk powder solution 2 % Trypsin solution Distilled water 5 cm3 syringes 4 boiling for the serial dilution 5 boiling tubes for the main practical Stopwatch/timer Marker pen PAG4.2 OCR Observation Sheet Method Note: In this activity you will measure the time taken for a reaction to reach a standardised endpoint. The rate of the reaction will be calculated as 1/time to end point. Serial Dilution: First you need to prepare the following concentrations of enzyme solution: 1.00%, 0.50%, 0.25% and 0.13% by using the 2.00% Trypsin solution and distilled water. Use the following diagram to help you: Label five boiling tubes with the Trypsin concentrations you have prepared and place 2 cm3 milk powder solution into each. Hold one of the tubes up against the Observation Sheet. You should not be able to see the text through the milk solution. Add 2 cm3 of 2.00% Trypsin solution to the boiling tube labelled 2.00% and immediately start the stopwatch. Hold the tube up against the Observation Sheet and stop the timer once the writing becomes visible. Record your result in a suitable table. Include a column for rate of reaction. Repeat steps 3 - 5 for the other four concentrations, 1.00%, 0.50%, 0.25% and 0.13%. Plot a graph of time versus enzyme concentration. Finally, plot a graph of rate of reaction versus enzyme concentration.
The effect of enzyme concentration on the rate of a reaction STUDENT Introduction Trypsin is a protease that catalyses the breakdown of other proteins. By making a serial dilution of Trypsin solution, you will observe the effect of changing enzyme concentration on the rate of a reaction. Aim To investigate the effect of changing the enzyme concentration on the rate of milk protein breakdown by Trypsin. Intended class time 1 hour Equipment Milk powder solution 2 % Trypsin solution Distilled water 5 cm3 syringes 4 boiling for the serial dilution 5 boiling tubes for the main practical Stopwatch/timer Marker pen PAG4.2 OCR Observation Sheet Method Note: In this activity you will measure the time taken for a reaction to reach a standardised endpoint. The rate of the reaction will be calculated as 1/time to end point. Serial Dilution: First you need to prepare the following concentrations of enzyme solution: 1.00%, 0.50%, 0.25% and 0.13% by using the 2.00% Trypsin solution and distilled water. Use the following diagram to help you: Label five boiling tubes with the Trypsin concentrations you have prepared and place 2 cm3 milk powder solution into each. Hold one of the tubes up against the Observation Sheet. You should not be able to see the text through the milk solution. Add 2 cm3 of 2.00% Trypsin solution to the boiling tube labelled 2.00% and immediately start the stopwatch. Hold the tube up against the Observation Sheet and stop the timer once the writing becomes visible. Record your result in a suitable table. Include a column for rate of reaction. Repeat steps 3 - 5 for the other four concentrations, 1.00%, 0.50%, 0.25% and 0.13%. Plot a graph of time versus enzyme concentration. Finally, plot a graph of rate of reaction versus enzyme concentration.
PAG4.2%20Student%20The%20effect%20of%20enzyme%20conc%20on%20the%20rate%20of%20a%20reaction_v0.1.docx