Before moving on to using the Observation Window, it should be noted: Look to the window at Try turning off the phase the right indicated by the contrast first. If most of green square. In this area the image is in phase the Operator can select contrast, a new sett of the cannels that the dyes needs to be image is being displayed selected that will cause on. By turning these the sample to fluoresce. channels on and off, very different images and features can be observed. When the Operator is DO NOT CLICK “Start” or ready to move on, click the acquisition process “NEXT”. start all over. Observation Window Layout The image directly above This is done by double is the image map created clicking anywhere on the during the previous image to focus the lasers steps. In this window the at that point. Operator can navigate around the sample to find Use the channel buttons points of interest. above the image to adjust which wavelength to be observed. Use the “Zoom” bar on the side to identify This avoids wasting time important features first. using the laser to look at the image; it is a slow process and it can damage the sample. Use the container selection tool, located at This changes the area of the very top of the the slide to be observed. window, to change the container. Using the “Repeat” At 1X the machine rasters The Repeat button button. at a standard speed and determines the rate at
Before moving on to using the Observation Window, it should be noted: Look to the window at the right indicated by the green square. In this area the Operator can select the cannels that the image is being displayed on. By turning these channels on and off, very different images and features can be observed. When the Operator is ready to move on, click “NEXT”. Try turning off the phase contrast first. If most of the image is in phase contrast, a new sett of dyes needs to be selected that will cause the sample to fluoresce. DO NOT CLICK “Start” or the acquisition process start all over. Observation Window Layout The image directly above is the image map created during the previous steps. In this window the Operator can navigate around the sample to find points of interest. This is done by double clicking anywhere on the image to focus the lasers at that point. Use the channel buttons above the image to adjust which wavelength to be observed. Use the “Zoom” bar on the side to identify important features first. Use the container selection tool, located at the very top of the window, to change the container. This avoids wasting time using the laser to look at the image; it is a slow process and it can damage the sample. This changes the area of the slide to be observed. Using the “Repeat” button. At 1X the machine rasters at a standard speed and The Repeat button determines the rate at
11_Confocal_Microscope_Operations.docx