Number: _____11__________________ WORK INSTRUCTION BREAKDOWN SHEET Laser Scanning Confocal Microscope Operation: Operations Laser Scanning Confocal Microscope Instrument: Olympus Fluoview FV10i IMPORTANT STEPS KEY POINTS REASONS WHY A logical segment of the Anything in a step that Reasons for each key operation when might— point something happens to Make or break the advance the work. job Injure the worker Be a Cultural Consideration Make the work easier to do, i.e. “knack”, “trick”, special timing, bit of special information Sample Stage Preparation Gather necessary If using small cylindrical resources. sample holders, use the setup to the right. Notice the different types of “sample locks” (which hold the samples firmly in place) and “sample sites” (where the sample is held on the stage during imaging). Gather necessary If using glass slides, use resources. the setup to the right. Notice the “oil deposition site” (where oil is deposited before using the 60x Objective) and the “stage key” (used to align to microscope when inserting stage). Place sample site on the For small cylindrical stage (small cylindrical sample holders, place the samples). sample onto the sample
Number: _____11__________________ WORK INSTRUCTION BREAKDOWN SHEET Operation: Laser Scanning Confocal Microscope Operations Instrument: Olympus Fluoview FV10i Laser Scanning Confocal Microscope IMPORTANT STEPS KEY POINTS REASONS WHY A logical segment of the operation when something happens to advance the work. Anything in a step that might— Make or break the job Injure the worker Be a Cultural Consideration Make the work easier to do, i.e. “knack”, “trick”, special timing, bit of special information Reasons for each key point Sample Stage Preparation Gather necessary resources. If using small cylindrical sample holders, use the setup to the right. Notice the different types of “sample locks” (which hold the samples firmly in place) and “sample sites” (where the sample is held on the stage during imaging). Gather necessary resources. If using glass slides, use the setup to the right. Notice the “oil deposition site” (where oil is deposited before using the 60x Objective) and the “stage key” (used to align to microscope when inserting stage). Place sample site on the stage (small cylindrical samples). For small cylindrical sample holders, place the sample onto the sample
11_Confocal_Microscope_Operations.docx