yields much clearer which the microscope images. rasters an image. At 4X the image is Raster speed does not generated much quicker, affect the final image; but the resolution is poor. however, it does influence the rate of photobleaching of the sample. A quicker rastering speed means more passes by the laser over the area of interest. More passes means more excitation which inevitably leads to photobleaching, resulting in an unimagable section of the sample. Select the channels that the image is being displayed on by using the feature illustrated at the By turning these channels on and off, very different right. images and features can be observed. When multiple dyes were selected, the Operator can only have two activated at one time during the image observation. As the operator switches between lasers, an image is generated that combines all the different channels into a single image. As the Operator moves Each square represents a The bottom right square around the map image, different wavelength combines all other the image will change being recorded. images into one. (ensure that “Repeat” is ON). By adding and removing This will not affect the channels, the individual final image capture. squares that are associated with those channels will alter the combined image. By clicking and dragging This allows for precise within these squares the feature alignment. Operator can move the focus of the laser. This section of the screen By clicking on the “10X” affects the depth of focus button, the Operator will
yields much clearer images. At 4X the image is generated much quicker, but the resolution is poor. which the microscope rasters an image. Raster speed does not affect the final image; however, it does influence the rate of photobleaching of the sample. A quicker rastering speed means more passes by the laser over the area of interest. More passes means more excitation which inevitably leads to photobleaching, resulting in an unimagable section of the sample. Select the channels that the image is being displayed on by using the feature illustrated at the right. By turning these channels on and off, very different images and features can be observed. When multiple dyes were selected, the Operator can only have two activated at one time during the image observation. As the operator switches between lasers, an image is generated that combines all the different channels into a single image. As the Operator moves around the map image, the image will change (ensure that “Repeat” is ON). Each square represents a different wavelength being recorded. By adding and removing channels, the individual squares that are associated with those channels will alter the combined image. By clicking and dragging within these squares the Operator can move the focus of the laser. The bottom right square combines all other images into one. This will not affect the final image capture. This allows for precise feature alignment. This section of the screen affects the depth of focus By clicking on the “10X” button, the Operator will
11_Confocal_Microscope_Operations.docx