Index Introduction Phosphoproteomic Research Sample preparation Phosphorylated protein enrichment Mass spectrometry analysis of phosphoproteins Materials and Method Reagents and materials Methods Sample preparation Phosphopeptide enrichment LC MS/MS Analysis – Data analysis Results and Discussion Optimization of enrichment Different MOAC materials Additives/buffers experimentation Additive optimization Elution optimization Identification techniques CID/ETD fragmentation Proton mobility Conclusion References
Index Introduction Phosphoproteomic Research Sample preparation Phosphorylated protein enrichment Mass spectrometry analysis of phosphoproteins Materials and Method Reagents and materials Methods Sample preparation Phosphopeptide enrichment LC – MS/MS Analysis Data analysis Results and Discussion Optimization of enrichment Different MOAC materials Additives/buffers experimentation Additive optimization Elution optimization Identification techniques CID/ETD fragmentation Proton mobility Conclusion References
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