BCR ABL EDTA Samples should be in Cancer Molecular 14-21 (Molecular Peripheral afternoon before 16:00 Diagnostics working days Analysis of blood or Ensure sample is (CMD)St. James' Leukaemia) BM in accompanied with fully completed Hospital RPMI and signed PATH-HAEM-EXT-14 For Chronic Cancer Molecular Myeloid for molecular analysis Leukaemia (CML) or disorders (MPD) Cytogenetics BM in All samples must be accompanied Division of Results RPMI with by a fully completed and signed Cytogenetics, available heparin for PATH-HAEM-EXT-5 NCMG National Centre for approx 6 Leuk/MDS Cytogenetics request form. Medical Genetics, months. BM in Samples should be in Our Lady's Urgent RPMI with afternoon and received in the Children's Hospital, referrals may laboratory before 16:00 Crumlin, Dublin have a 12.(NCMG) preliminary Blood in FISH result EDTA for within 4 days CLL BM smears for Multiple Myeloma Chimerism PB/BM Samples should be taken in Cancer Molecular Usually within testing 9ml of afternoon and received in the Diagnostics, Central 14-21 peripheral laboratory before 16:00 Pathology working blood in Laboratory, St days. EDTA (3 Ensure sample is accompanied James's Hospital, samples) with fully completed and signed Dublin 8. or 1-3ml of PATH-HAEM-EXT-14 Cancer bone Molecular Diagnostics-Request for marrow in molecular analysis RPMI (1:3 dilution) CD3 3ml blood Take sample after 2 pm Immunology Lab, 5 working CD4 in EDTA X Contact lab prior to St. James Hospital days (T Helper), CD8 2 sending samples as it must arrive Dublin 8 (T Cytotoxic), in referral laboratory within 24 CD19 hours of phlebotomy. Time and (B cell), date of collection must be on form. CD16/56 (NK cells).
BCR ABL (Molecular Analysis of Leukaemia) For Chronic Myeloid Leukaemia (CML) or disorders (MPD) EDTA Peripheral blood or BM in RPMI Samples should be in afternoon before 16:00 Ensure sample is accompanied with fully completed and signed PATH-HAEM-EXT-14 Cancer Molecular for molecular analysis Cancer Molecular Diagnostics (CMD)St. James' Hospital 14-21 working days Cytogenetics BM in RPMI with heparin for Leuk/MDS BM in RPMI with Blood in EDTA for CLL BM smears for Multiple Myeloma All samples must be accompanied by a fully completed and signed PATH-HAEM-EXT-5 NCMG Cytogenetics request form. Samples should be in afternoon and received in the laboratory before 16:00 Division of Cytogenetics, National Centre for Medical Genetics, Our Lady's Children's Hospital, Crumlin, Dublin 12.(NCMG) Results available approx 6 months. Urgent referrals may have a preliminary FISH result within 4 days Chimerism testing PB/BM 9ml of peripheral blood in EDTA (3 samples) or 1-3ml of bone marrow in RPMI (1:3 dilution) Samples should be taken in afternoon and received in the laboratory before 16:00 Ensure sample is accompanied with fully completed and signed PATH-HAEM-EXT-14 Cancer Molecular Diagnostics-Request for molecular analysis Cancer Molecular Diagnostics, Central Pathology Laboratory, St James's Hospital, Dublin 8. Usually within 14-21 working days. CD3 CD4 (T Helper), CD8 (T Cytotoxic), CD19 (B cell), CD16/56 (NK cells). 3ml blood in EDTA X 2 Take sample after 2 pm Contact lab prior to sending samples as it must arrive in referral laboratory within 24 hours of phlebotomy. Time and date of collection must be on form. Immunology Lab, St. James Hospital Dublin 8 5 working days
pathology-user-handbook-2018-revision-12.docx