6.2 mmol/L) N=2 HbS non-diabetic (mean FPG = 5.3 mmol/L) Schnedl () Enzymatic BA-HPLC IE-HPLC analysers Immunoassays The enzymatic and boronate affinity HPLC Austria Arkray Primus HA8160 HLC-723 DCA Tina Unimate Rapida method did not interfere with any of the variants N=2 Hb Graz diabetic 8.7 8.55 No result No result 5.85 5.8 5.9 5.15 evaluated. (mean FPG = No result No result 4.05 4.35 4.05 2.6 Hb Graz interfered with all immunoassay and 13.7 mmol/L) 5.2 5.35 No result 2.8 4.7 5.2 5.1 4.4 ion-exchange HPLC methods evaluated. N=2 Hb Graz 5.1 4.8 4.1 3.5 6.2 5.7 5.9 5.7 The Tosoh ion-exchange HPLC method 5.1 5.4 5.9 7.6 7.8 7.6 8.0 8.3 did not detect the late migrating (mean FPG = 7.3 7.6 3.8 5.4 5.8 5.4 5.7 5.6 in the chromatogram, but this 4.8 mmol/L) 5.9 5.3 haemoglobin variant still interfered, causing N=1 Hb Sherwood artificially low HbA1c results. Forest (mean FPG = 5.2 mmol/L) N=1 HbD diabetic (mean FPG = 5.9 mmol/L) N=1 HbO-Padova diabetic (mean FPG = 5.9 mmol/L) N=1 HbO-Padova normal (mean FPG = 6.1 mmol/L)
6.2 mmol/L) N=2 HbS non-diabetic (mean FPG = 5.3 mmol/L) Schnedl () Austria N=2 Hb Graz diabetic (mean FPG = 13.7 mmol/L) N=2 Hb Graz (mean FPG = 4.8 mmol/L) N=1 Hb Sherwood Forest (mean FPG = 5.2 mmol/L) N=1 HbD diabetic (mean FPG = 5.9 mmol/L) N=1 HbO-Padova diabetic (mean FPG = 5.9 mmol/L) N=1 HbO-Padova normal (mean FPG = 6.1 mmol/L) Enzymatic BA-HPLC Arkray Primus 8.7 8.55 5.2 5.35 5.1 4.8 5.1 5.4 7.3 7.6 5.9 5.3 IE-HPLC analysers HA8160 HLC-723 No result No result No result No result No result 2.8 4.1 3.5 5.9 7.6 3.8 5.4 Immunoassays DCA Tina Unimate Rapida 5.85 5.8 5.9 5.15 4.05 4.35 4.05 2.6 4.7 5.2 5.1 4.4 6.2 5.7 5.9 5.7 7.8 7.6 8.0 8.3 5.8 5.4 5.7 5.6 The enzymatic and boronate affinity HPLC method did not interfere with any of the variants evaluated. Hb Graz interfered with all immunoassay and ion-exchange HPLC methods evaluated. The Tosoh ion-exchange HPLC method did not detect the late migrating in the chromatogram, but this haemoglobin variant still interfered, causing artificially low HbA1c results.
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