Gatorade 5.48 0.792 Powerade 4.79 0.801 Calculations Discussion The enzyme reactions were observed on the breakdown and production of energy. Using energy it is possible to determine the rate at which enzymes function. The basis for experiment was to test for the presence of NADPH (energy) at timed intervals. A pre-determined wavelength of 340nm was used. Recordings for NADPH absorbencies were recorded in Tables 1 and 2 as and columns. ‘A1’ ‘A2’ Figure 1 and 2 used several standardised figures 0mM, 2mM, 4mM and 6mM which allowed a – – comparative analysis of the sample data. By performing the steps outlined in the lab manual, two assays were completed successfully. Table1 presents recordings that were used to compare sucrose and glucose levels whereas Table 2 refers only to naturally occurring glucose levels. The difference between the two is needed as they both provide naturally occurring glucose for an immediate energy response in the body Table 2. Sucrose is made up – of (also known as a fruit sugar) and glucose. In order for glucose levels to be obtained the ‘fructose’ sucrose was broken down using hydrolysis. (Marieb, 2007). The fructose within the sucrose is needed as it provides a delayed energy source via the liver’s glycogen cycle due to its lacking adherence initially. Table 3 shows the sucrose/glucose grams per 100mL for each sample.
Gatorade 5.48 0.792 Powerade 4.79 0.801 Calculations Discussion The enzyme reactions were observed on the breakdown and production of energy. Using energy it is possible to determine the rate at which enzymes function. The basis for experiment was to test for the presence of NADPH (energy) at timed intervals. A pre-determined wavelength of 340nm was used. Recordings for NADPH absorbencies were recorded in Tables 1 and 2 as ‘A1’ and ‘A2’ columns. Figure 1 and 2 used several standardised figures – 0mM, 2mM, 4mM and 6mM – which allowed a comparative analysis of the sample data. By performing the steps outlined in the lab manual, two assays were completed successfully. Table1 presents recordings that were used to compare sucrose and glucose levels whereas Table 2 refers only to naturally occurring glucose levels. The difference between the two is needed as they both provide naturally occurring glucose for an immediate energy response in the body – Table 2. Sucrose is made up of ‘fructose’ (also known as a fruit sugar) and glucose. In order for glucose levels to be obtained the sucrose was broken down using hydrolysis. (Marieb, 2007). The fructose within the sucrose is needed as it provides a delayed energy source via the liver’s glycogen cycle due to its lacking adherence initially. Table 3 shows the sucrose/glucose grams per 100mL for each sample.
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