Remember that the UV exposed plates originally contained approximately 1000 cells. The control plates contained about 100 cells each. The control plates were plated with a different dilution so that the colony numbers would not be too great to count. Since all of the dilutions started from the same source, the UV and control plates should be different by factors of 10. For this reason, we multiply the average number of colonies on the control plates by 10 to estimate the actual number of colonies that were initially on the UV exposed plates. YED YED 8 10 1 2 Day seven: Prepare Test Plates Overview: Test plates will be prepared from all or most of the red mutants produced by all classes. The mutations can be used as tools to investigate how a yeast cell works. Our first step in this process is to determine the inheritance patterns of these red mutants. Prepare a test plate of the suspected mutants from both mating types on YED media. Use the master plates that your instructor has made from the original mutants that your class prepared earlier. Use separate, sterile toothpicks to set up your plate as in Figure 2. Make sure you clearly label the bottom of your test plate. Your number of mutants will probably vary, but don't put more than 3 or 4 unknowns for either mating type. There probably isn't room on the plate for all of the mating mixes. Incubate 1-2 days.
Remember that the UV exposed plates originally contained approximately 1000 cells. The control plates contained about 100 cells each. The control plates were plated with a different dilution so that the colony numbers would not be too great to count. Since all of the dilutions started from the same source, the UV and control plates should be different by factors of 10. For this reason, we multiply the average number of colonies on the control plates by 10 to estimate the actual number of colonies that were initially on the UV exposed plates. YED YED 8 10 1 2 Day seven: Prepare Test Plates Overview: Test plates will be prepared from all or most of the red mutants produced by all classes. The mutations can be used as tools to investigate how a yeast cell works. Our first step in this process is to determine the inheritance patterns of these red mutants. Prepare a test plate of the suspected mutants from both mating types on YED media. Use the master plates that your instructor has made from the original mutants that your class prepared earlier. Use separate, sterile toothpicks to set up your plate as in Figure 2. Make sure you clearly label the bottom of your test plate. Your number of mutants will probably vary, but don't put more than 3 or 4 unknowns for either mating type. There probably isn't room on the plate for all of the mating mixes. Incubate 1-2 days.
FE%20Pilot_%20Mutant%20Hunt%20SM_5-3-11.docx