Cytogenetics BM in All samples must be accompanied Division of Results RPMI with by a fully completed and signed Cytogenetics, available heparin for PATH-HAEM-EXT-5 NCMG National Centre for approx 6 Leuk/MDS Cytogenetics request form. Medical Genetics, months. BM in Samples should be taken in Our Lady's Urgent RPMI with afternoon and received in the Children's Hospital, referrals may laboratory before 16:00 Crumlin, Dublin have a 12.(NCMG) preliminary in FISH result EDTA for within 4 days CLL BM smears for Multiple Myeloma Chimerism PB/BM Samples should be taken in Cancer Molecular Usually within testing 9ml of afternoon and received in the Diagnostics, Central 14-21 peripheral laboratory before 16:00 Pathology working blood in Laboratory, St days. EDTA (3 Ensure sample is accompanied James's Hospital, samples) with fully completed and signed Dublin 8. or 1-3ml of PATH-HAEM-EXT-14 Cancer bone Molecular Diagnostics-Request for marrow in molecular analysis RPMI (1:3 dilution) CD3 (cells), 3ml blood Take sample 2 pm Immunology Lab, 5 working CD4 in EDTA X Contact lab prior to St. Hospital days (T Helper), CD8 2 sending samples as it must arrive Dublin 8 (T Cytotoxic), in referral laboratory within 24 CD19 hours of phlebotomy. Time and (B cell), date of collection must be on form. CD16/56 (NK cells). Factor Assays Na citrate Send samples Mon-Fri. Ensure St James's 6-8 weeks x 6 delivered to laboratory within 4 Hospital. hours post phlebotomy and during For patients <16 yrs routine hours send to OLCHC Crumlin Factor inhibitor Na citrate Send samples Mon-Fri. Ensure NCHCD St James's 4 weeks test x delivered to laboratory within 4 Hospital. 2 post phlebotomy and during routine For patients <16 yrs hours send to OLCHC Crumlin Factor V Leiden 1 x EDTA Note: Factor V Leiden testing is Biomnis 8 days only carried out on patients with an abnormally reduced APCR result
Cytogenetics BM in RPMI with heparin for Leuk/MDS BM in RPMI with in EDTA for CLL BM smears for Multiple Myeloma All samples must be accompanied by a fully completed and signed PATH-HAEM-EXT-5 NCMG Cytogenetics request form. Samples should be taken in afternoon and received in the laboratory before 16:00 Division of Cytogenetics, National Centre for Medical Genetics, Our Lady's Children's Hospital, Crumlin, Dublin 12.(NCMG) Results available approx 6 months. Urgent referrals may have a preliminary FISH result within 4 days Chimerism testing PB/BM 9ml of peripheral blood in EDTA (3 samples) or 1-3ml of bone marrow in RPMI (1:3 dilution) Samples should be taken in afternoon and received in the laboratory before 16:00 Ensure sample is accompanied with fully completed and signed PATH-HAEM-EXT-14 Cancer Molecular Diagnostics-Request for molecular analysis Cancer Molecular Diagnostics, Central Pathology Laboratory, St James's Hospital, Dublin 8. Usually within 14-21 working days. CD3 (cells), CD4 (T Helper), CD8 (T Cytotoxic), CD19 (B cell), CD16/56 (NK cells). 3ml blood in EDTA X 2 Take sample 2 pm Contact lab prior to sending samples as it must arrive in referral laboratory within 24 hours of phlebotomy. Time and date of collection must be on form. Immunology Lab, St. Hospital Dublin 8 5 working days Factor Assays Na citrate x 6 Send samples Mon-Fri. Ensure delivered to laboratory within 4 hours post phlebotomy and during routine hours St James's Hospital. For patients <16 yrs send to OLCHC Crumlin 6-8 weeks Factor inhibitor test Na citrate x 2 Send samples Mon-Fri. Ensure delivered to laboratory within 4 post phlebotomy and during routine hours NCHCD St James's Hospital. For patients <16 yrs send to OLCHC Crumlin 4 weeks Factor V Leiden 1 x EDTA Note: Factor V Leiden testing is only carried out on patients with an abnormally reduced APCR result Biomnis 8 days
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