USA. Genotype imputation was performed using IMPUTE2 and a reference panel from the 1000 Genomes. The study was approved by the Joint Commission on Ethics of the Turku University and the Turku University Central Hospital. Informed consent was obtained from all parents at the beginning of the trial and from the children at 15 years of age. Cardiovascular Risk in Young Finns Study (YFS) YFS is a large prospective population-based multicentre study aimed at examining the risk factors and their determinants in children and adolescents of risk factors for atherosclerosis. The baseline cross-sectional study was carried out in 1980, including 3596 subjects at ages 3, 6, 9, 12, 15, and 18. Between 1980 and 1992, these subjects were followed up at 3-year intervals, and then as adults in 2001, 2007 and 2010-2012. The main aim of the YFS is to determine the contribution made by childhood lifestyle, biological and psychological measures to the risk of cardiovascular diseases in adulthood. Genotyping was performed with the Illumina 670K SNP chip at the Wellcome Trust Sanger Centre. Genotype imputation was performed using MACH 1.0 and HapMap II CEU (release 22) samples. Quality control measures were taken prior to imputation (excluding genotypes with call rate <0.95, MAF <0.01, HWE <1 x 10-6). P-value All subjects gave their written informed consent in 2007 and the study was approved by local ethics committees of the participating universities. References
USA. Genotype imputation was performed using IMPUTE2 and a reference panel from the 1000 Genomes. The study was approved by the Joint Commission on Ethics of the Turku University and the Turku University Central Hospital. Informed consent was obtained from all parents at the beginning of the trial and from the children at 15 years of age. Cardiovascular Risk in Young Finns Study (YFS) YFS is a large prospective population-based multicentre study aimed at examining the risk factors and their determinants in children and adolescents of risk factors for atherosclerosis. The baseline cross-sectional study was carried out in 1980, including 3596 subjects at ages 3, 6, 9, 12, 15, and 18. Between 1980 and 1992, these subjects were followed up at 3-year intervals, and then as adults in 2001, 2007 and 2010-2012. The main aim of the YFS is to determine the contribution made by childhood lifestyle, biological and psychological measures to the risk of cardiovascular diseases in adulthood. Genotyping was performed with the Illumina 670K SNP chip at the Wellcome Trust Sanger Centre. Genotype imputation was performed using MACH 1.0 and HapMap II CEU (release 22) samples. Quality control measures were taken prior to imputation (excluding genotypes with call rate <0.95, MAF <0.01, HWE P-value <1 x 10-6). All subjects gave their written informed consent in 2007 and the study was approved by local ethics committees of the participating universities. References
Felix_ddv472supp.docx