10-1 plate from the first (repeated) round of Figure 5 Empirical Assay 1 – purification of Eye. The two different Bull’s X ½ empirical assay plate from the first trial. plaque sizes are visible on this plate. Note the varied plaque morphologies. Figure 2 Spot Test – Figure 6 Electrophoresis 1 – The initial spot test to confirm putative The first gel electrophoresis. Lanes from left plaques as actual plaques and not anomalies to right: Ladder, Uncut, Bam HI, Cla I, Eco on the enrichment plates. Fuzzy bled into the RI, Hae III, and HindI III. Note there are no positive control so it was disregarded. Tex bands on Hae III. and Eye were used for successive Bull’s purifications. Figure 7 Electrophoresis Gel 2 – Figure 4-A Second Purification A – Gel electrophoresis trial number 2. Lanes 10-2 plate from the second round of from left to right: Ladder, Uncut, Bam HI, purification of Eye. This plate was Bull’s Cla I, Eco RI, Hae III, and HindI III, Pst I. made from a plaque that was 5 mm in Cuts on Hae III and Pst I. diameter. The plaque sizes varied between 4 Figure 8-A EM picture A – mm and 1 mm in diameter. Clearly defined phage in middle of picture showing both negative and positive staining. Note the round in bottom left corner “heads” Figure 4-B Second Purification B have no tails attached. – 10-2 plate from the second round of Figure 8-B EM picture B – purification of Eye. This plate was Bull’s Three well defined phage in the middle of made from a plaque that was 2 mm in the image. Note the heads scattered around diameter. The plaque sizes varied between the edges of the frame. the 4 mm and 1 mm in diameter. Figure 9 Glimmer predictions in GBrowse – There are approximately 80 Glimmer predicted genes in genome. Timshel’s
10-1 plate from the first (repeated) round of purification of Bull’s Eye. The two different plaque sizes are visible on this plate. Figure 2 – Spot Test The initial spot test to confirm putative plaques as actual plaques and not anomalies on the enrichment plates. Fuzzy bled into the positive control so it was disregarded. Tex and Bull’s Eye were used for successive purifications. Figure 4-A – Second Purification A 10-2 plate from the second round of purification of Bull’s Eye. This plate was made from a plaque that was 5 mm in diameter. The plaque sizes varied between 4 mm and 1 mm in diameter. Figure 4-B – Second Purification B 10-2 plate from the second round of purification of Bull’s Eye. This plate was made from a plaque that was 2 mm in diameter. The plaque sizes varied between the 4 mm and 1 mm in diameter. Figure 5 – Empirical Assay 1 X ½ empirical assay plate from the first trial. Note the varied plaque morphologies. Figure 6 – Electrophoresis 1 The first gel electrophoresis. Lanes from left to right: Ladder, Uncut, Bam HI, Cla I, Eco RI, Hae III, and HindI III. Note there are no bands on Hae III. Figure 7 – Electrophoresis Gel 2 Gel electrophoresis trial number 2. Lanes from left to right: Ladder, Uncut, Bam HI, Cla I, Eco RI, Hae III, and HindI III, Pst I. Cuts on Hae III and Pst I. Figure 8-A – EM picture A Clearly defined phage in middle of picture showing both negative and positive staining. Note the round “heads” in bottom left corner have no tails attached. Figure 8-B – EM picture B Three well defined phage in the middle of the image. Note the heads scattered around the edges of the frame. Figure 9 – Glimmer predictions in GBrowse There are approximately 80 Glimmer predicted genes in Timshel’s genome.
erin%20harvey%20Research%20Paper.docx