funnel and extract with 25 mL of ether twice. 6.9 Collect the ether layers of all the three separating funnels into one. 6.10 Wash the ether layer with water to remove any traces of mineral acid 6.11 Transfer ether extract to a 500 mL beaker and evaporate to dryness on water bath. 6.12 Dissolve the residue in 50 mL neutralized methanol and titrate with standard0.1 N NaOH solution using phenolphthalein indicator 6.13 End point for titration is when the color changes from mL mL colorless to pale pink. 7. Calculation: Wt. of KHP X 1000 Normality of NaOH = ---------------------------------------------------------------------------- Volume of NaOH consumed X Eq. wt of KHP(204.22) Where, V × N ×18.62 V=Volume in mL of standard NaOH solution consumed Active ingredient, = ------------------- for the sample % by mass M N=Normality of the standard NaOH solution M=Mass in g of the sample taken for test Result: Sl. No. Name of Test Result Unit Method of Analysis 1. Active ingredient (Alpha NAA) % IS 13138:1991 Reference in Daily workbook: Name Analyzed by Dated signature Name Checked by
funnel and extract with 25 mL of ether twice. 6.9 Collect the ether layers of all the three separating funnels into one. 6.10 Wash the ether layer with water to remove any traces of mineral acid 6.11 Transfer ether extract to a 500 mL beaker and evaporate to dryness on water bath. 6.12 Dissolve the residue in 50 mL neutralized methanol and titrate with standard0.1 N NaOH solution using phenolphthalein indicator 6.13 End point for titration is when the color changes from colorless to pale pink. mL mL 7. Calculation: Wt. of KHP X 1000 Normality of NaOH = ---------------------------------------------------------------------------- Volume of NaOH consumed X Eq. wt of KHP(204.22) V × N ×18.62 Active ingredient, = ------------------- % by mass M Where, V=Volume in mL of standard NaOH solution consumed for the sample N=Normality of the standard NaOH solution M=Mass in g of the sample taken for test Result: Sl. No. Name of Test Result Unit Method of Analysis 1. Active ingredient (Alpha NAA) % IS 13138:1991 Reference in Daily workbook: Analyzed by Name Dated signature Checked by Name
FC-PF-246(AlphaNAA)-Rev02.docx