Comparator results or were sputum scarce Reference std Exclusion Flow and timing Patients who were Applicability: C1, P2 AFB +ve or HIV+ or were missing culture specimens George, Mony Level III-2: N=78 sputum samples Inclusion Sputum samples were LAMP assay specific for AUR fluorescence L-J culture and MGIT & Kenneth () decontaminated using the rimM sequence of microscopy culture A comparison with Age and HIV status not TB suspects NALC-NaOH method MTB and Mycobacterium India reference standard (not reported Exclusion and stored at –20 °C bovis blinded or blinding not Not reported Decontaminated known) sputum was processed Quality: Low risk of bias using the Amplicor Patient selection respiratory specimen Index test preparation kit Comparator Reference std ? Flow and timing Applicability: C1, P2 Ghaleb, Afifi & Level III-2: N=100 urine samples Inclusion Urine specimens were PCR targeting IS6110 AFB microscopy L-J solid and El-Gohary () treated with with ZN staining BACTEC 12B liquid A comparison with 75 males with mean Patients with method for the culture Egypt reference standard (not age 37.5 ±7.5 years symptoms suggestive decontamination blinded or blinding not of renal TB 25 females with mean known) age 37.0 ± 9.0 years Exclusion Quality: Some risk of bias HIV status not reported None reported Patient selection Index test ? Comparator Reference std ? Flow and timing Applicability: C1, P2
Comparator Reference std Flow and timing Applicability: C1, P2 results or were sputum scarce Exclusion Patients who were AFB +ve or HIV+ or were missing culture specimens George, Mony & Kenneth () India Level III-2: A comparison with reference standard (not blinded or blinding not known) Quality: Low risk of bias Patient selection Index test Comparator Reference std ? Flow and timing Applicability: C1, P2 N=78 sputum samples Age and HIV status not reported Inclusion TB suspects Exclusion Not reported Sputum samples were decontaminated using NALC-NaOH method and stored at –20 °C Decontaminated sputum was processed using the Amplicor respiratory specimen preparation kit LAMP assay specific for the rimM sequence of MTB and Mycobacterium bovis AUR fluorescence microscopy L-J culture and MGIT culture Ghaleb, Afifi & El-Gohary () Egypt Level III-2: A comparison with reference standard (not blinded or blinding not known) Quality: Some risk of bias Patient selection Index test ? Comparator Reference std ? Flow and timing Applicability: C1, P2 N=100 urine samples 75 males with mean age 37.5 ±7.5 years 25 females with mean age 37.0 ± 9.0 years HIV status not reported Inclusion Patients with symptoms suggestive of renal TB Exclusion None reported Urine specimens were treated with method for the decontamination PCR targeting IS6110 AFB microscopy with ZN staining L-J solid and BACTEC 12B liquid culture
1234_FinalAssessmentReport-accessible.docx