WT : Met Arg Phe – – – Thr….. Mutant 1: Met Ile Phe – – – Thr ….. Mutant 2: Met Ser Ile - – – Tyr….. 5 An Escherichia coli mutant auxotroph for tryptophan (Trp-) has an amino acid substitution in tryptophan-synthetase: glycine in position 210 is replaced by an arginine. On the basis of the genetic code, individuate which kind of mutation (on the DNA) you think has caused the amino acid replacement. 6 - In Escherichia coli metA gene a base substitution occurred. Because of this mutation, in the mRNA a UAA codon is present inside the gene. - Which consequences will have this mutation on protein synthesis? 7 - The primary transcript of chicken ovalbumin RNA is composed by 7 introns (white) and 8 exons (black): - If the Ovoalbumin DNA is isolated, denaturated and hybridized with its cytoplasmatic mRNA, which kind of structure we expect? - if a deletion of a base pair occurs in the middle of the second intron, which will be the likely effects on the resulting polypeptide? - if a deletion of a base pair occurs in the middle of the first exon, which will be the likely effects on the resulting polypeptide?
WT : Met – Arg – Phe – Thr….. Mutant 1: Met – Ile – Phe – Thr ….. Mutant 2: Met – Ser – Ile - Tyr….. 5 An Escherichia coli mutant auxotroph for tryptophan (Trp-) has an amino acid substitution in tryptophan-synthetase: glycine in position 210 is replaced by an arginine. On the basis of the genetic code, individuate which kind of mutation (on the DNA) you think has caused the amino acid replacement. 6 - In Escherichia coli metA gene a base substitution occurred. Because of this mutation, in the mRNA a UAA codon is present inside the gene. - Which consequences will have this mutation on protein synthesis? 7 - The primary transcript of chicken ovalbumin RNA is composed by 7 introns (white) and 8 exons (black): - If the Ovoalbumin DNA is isolated, denaturated and hybridized with its cytoplasmatic mRNA, which kind of structure we expect? - if a deletion of a base pair occurs in the middle of the second intron, which will be the likely effects on the resulting polypeptide? - if a deletion of a base pair occurs in the middle of the first exon, which will be the likely effects on the resulting polypeptide?
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