Please fill in which antibodies will be used, and either highlight, delete all others, or make known somehow which fluorochromes will be used. Put your antibody or stain in the “Target” column. Laser Filter Set Default Equivalent Equivalent Equivalent Equivalent Equivalent Target Target Red 633 nm Panel 1 Panel 2 C 660/20 APC Alexa Fluor 647 B 730/45 APC-Alexa DRAQ5 685 LP 700 680 A 780/60 APC-Cy7 APC-H7 Alexa 735 LP Fluor 750 Blue 488 nm C 488/10 SSC B** 530/30 FITC GFP Alexa CFSE YFP Sytox 502 LP Fluor 488 Green A* 695/40 PerCP PerCP PercPVio- 655 LP Cy5.5 eFluor 700 710 450 * 550/30 replaced **520/20 replaced 530 LP for better 502 LP for better can be resolution can be resolution of YFP of GFP Violet 407nm G 450/50 Pacific DAPI or Indo-1 Brilliant V450 Blue Hoechst (Violet) 450 Violet 421 F 530/30 BV510 V500 Pac Alexa 502LP Orange Fluor 430 E 582/15 BV570 530LP D 605/40 eFluor605 Qdot605 BV605 Evolve605 595LP C 660/40 BV650 eFluor650 Evolve655 630 LP B 710/50 BV 711 Qdot 705 690 LP A 780/60 BV786 750 LP Yellow/Green 561nm E 585/42 PE TdTomato AF 555 556LP D 616/23 PI PE -Texas mCherry DsRed mRuby 610LP Red C 670/14 PE-Cy5 7-AAD 630LP B 710/40 PE-Cy5.5 680 LP A 780/60 PE-Cy7 PE- 735LP Vio770
Please fill in which antibodies will be used, and either highlight, delete all others, or make known somehow which fluorochromes will be used. Put your antibody or stain in the “Target” column. Laser Filter Set Default Equivalent Equivalent Equivalent Equivalent Equivalent Target Target Red 633 nm Panel 1 Panel 2 C 660/20 APC Alexa Fluor 647 B 730/45 685 LP 700 APC-Alexa 680 DRAQ5 A 780/60 735 LP APC-Cy7 APC-H7 Alexa Fluor 750 Blue 488 nm C 488/10 SSC B** 530/30 502 LP FITC GFP Alexa Fluor 488 CFSE YFP Sytox Green A* 695/40 655 LP PerCP Cy5.5 PerCP 710 450 PercPVio- 700 * 550/30 530 LP can be replaced for better resolution of YFP **520/20 502 LP can be replaced for better resolution of GFP Violet 407nm G 450/50 Pacific Blue DAPI or Hoechst Indo-1 (Violet) eFluor 450 Brilliant Violet 421 V450 F 530/30 502LP BV510 V500 Pac Orange Alexa Fluor 430 E 582/15 530LP BV570 D 605/40 595LP eFluor605 Qdot605 BV605 Evolve605 C 660/40 630 LP BV650 eFluor650 Evolve655 B 710/50 690 LP BV 711 Qdot 705 A 780/60 750 LP BV786 Yellow/Green 561nm E 585/42 556LP PE TdTomato AF 555 D 616/23 610LP PI PE -Texas Red mCherry DsRed mRuby C 670/14 630LP PE-Cy5 7-AAD B 710/40 680 LP PE-Cy5.5 A 780/60 735LP PE-Cy7 PE- Vio770
FACS%20AriaIIIu%20use%20request%20form%20.docx