Features of cloning vector: Origin of replication (Ori), selectable marker and cloning sites are the features that are required to facilitate cloning into a vector. Origin of Replication (Ori) : This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. Selectable Marker : It is a gene which helps in identifying and eliminating transformants from (having recombinant DNA) by selectively permitting the growth of transformants. The process through which a piece of DNA is introduced in a host bacterium is called transformation. The genes encooling resistance to antibiotics are considered useful selectable marker for E.coli
Features of cloning vector: Origin of replication (Ori), selectable marker and cloning sites are the features that are required to facilitate cloning into a vector. Origin of Replication (Ori) : This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. Selectable Marker : It is a gene which helps in identifying and eliminating from transformants (having recombinant DNA) by selectively permitting the growth of transformants. The process through which a piece of DNA is introduced in a host bacterium is called transformation. The genes encooling resistance to antibiotics are considered useful selectable marker for E.coli
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