Applicability: C1, P1 Khan, Level III-2: N=50 urine samples Inclusion Urine specimens were PCR, details not AFB microscopy Culture on L-J Cheema & treated using provided with ZN staining medium A comparison with Median age of patients Patients with Khan () method for the reference standard (not symptoms suggestive 38 years (range 20–76) decontamination Egypt blinded or blinding not of GUTB HIV status not reported known) Exclusion Quality: Some risk of bias None reported Patient selection Index test ? Comparator ? Reference std ? Flow and timing ? Applicability: C1, P2 Khosravi et al. Level III-2: N=200 urine samples Inclusion Three urine samples nPCR targeting IS6110 AFB microscopy Culture on L-J () collected on three with ZN staining medium with a A comparison with Mean age 37.8 years Patients with consecutive days as conventional Iran reference standard (not symptoms suggestive HIV status not reported early morning urine, identification blinded or blinding not of renal TB pooled and procedure known) Exclusion concentrated Quality: Some risk of bias None reported Patient selection Index test ? Comparator Reference std ? Flow and timing Applicability: C1, P2 Kibiki et al. () Level III-2: N=120 BAL samples Inclusion BAL samples were PCR (40 cycles) Direct smears MTB culture was from 120 HIV+ patients pre-treated by targeting IS6110 were examined performed using Tanzania A comparison with HIV+ patients aged decontamination with for AFB after ZN L-J solid reference standard (not Mean age 39 years > 17 years with and staining medium, with a blinded or blinding not features of chest centrifuged maximum known) infection and referred incubation period of for bronchoscopy The sediment was Quality: Some risk of bias 8 weeks > 80% had previous used for the different
Applicability: C1, P1 Khan, Cheema & Khan () Egypt Level III-2: A comparison with reference standard (not blinded or blinding not known) Quality: Some risk of bias Patient selection Index test ? Comparator ? Reference std ? Flow and timing ? Applicability: C1, P2 N=50 urine samples Median age of patients 38 years (range 20–76) HIV status not reported Inclusion Patients with symptoms suggestive of GUTB Exclusion None reported Urine specimens were treated using method for the decontamination PCR, details not provided AFB microscopy with ZN staining Culture on L-J medium Khosravi et al. () Iran Level III-2: A comparison with reference standard (not blinded or blinding not known) Quality: Some risk of bias Patient selection Index test ? Comparator Reference std ? Flow and timing Applicability: C1, P2 N=200 urine samples Mean age 37.8 years HIV status not reported Inclusion Patients with symptoms suggestive of renal TB Exclusion None reported Three urine samples collected on three consecutive days as early morning urine, pooled and concentrated nPCR targeting IS6110 AFB microscopy with ZN staining Culture on L-J medium with a conventional identification procedure Kibiki et al. () Tanzania Level III-2: A comparison with reference standard (not blinded or blinding not known) Quality: Some risk of bias N=120 BAL samples from 120 HIV+ patients Mean age 39 years Inclusion HIV+ patients aged > 17 years with features of chest infection and referred for bronchoscopy > 80% had previous BAL samples were pre-treated by decontamination with and centrifuged The sediment was used for the different PCR (40 cycles) targeting IS6110 Direct smears were examined for AFB after ZN staining MTB culture was performed using L-J solid medium, with a maximum incubation period of 8 weeks
1234_FinalAssessmentReport-accessible.docx