Observation period: 3 days Method of administration: 0.5 ml of MCP 1440 was applied to a 2.5 cm2 shaved area on the anterior and posterior flanks of each animal; the test sites were occluded with Webril patches followed by a rubber dam and surgical tape; anterior patches were removed after 1 hour (corrosion test) and posterior patches after 4 hours (irritation test); test sites were wiped clean after patch removal Corrosion evaluation: both 1 and 4 hour occluded test sites showed no ulceration or necrosis immediately after patch removal or at 48 hours Irritation evaluation - Draize scores (9): Animal Time after decontamination (4 hour occlusion) 30 min 1 day 2 days 3 days Erythema 1 1i 1 0 0 2 1 0 0 0 3 1 0 0 0 4 1 0 0 0 5 1 0 0 0 6 1 1 0 0 Oedema 1 0 0 0 0 2 0 0 0 0 3 0 0 0 0 4 0 0 0 0 5 0 0 0 0 6 1 0 0 0 i see Attachment 1 for Draize scales Test Method: based on USEPA Health Effects Testing Guidelines (8) Result: slightly irritating, non-corrosive to rabbit skin 9.1.5 Eye Irritation (6) Species/strain: New Zealand White rabbits
Observation period: 3 days Method of administration: 0.5 ml of MCP 1440 was applied to a 2.5 cm2 shaved area on the anterior and posterior flanks of each animal; the test sites were occluded with Webril patches followed by a rubber dam and surgical tape; anterior patches were removed after 1 hour (corrosion test) and posterior patches after 4 hours (irritation test); test sites were wiped clean after patch removal Corrosion evaluation: both 1 and 4 hour occluded test sites showed no ulceration or necrosis immediately after patch removal or at 48 hours Irritation evaluation - Draize scores (9): Animal Time after decontamination (4 hour occlusion) 30 min 1 day 2 days 3 days Erythema 1 1i 1 0 0 2 1 0 0 0 3 1 0 0 0 4 1 0 0 0 5 1 0 0 0 6 1 1 0 0 Oedema 1 0 0 0 0 2 0 0 0 0 3 0 0 0 0 4 0 0 0 0 5 0 0 0 0 6 1 0 0 0 i see Attachment 1 for Draize scales Test Method: based on USEPA Health Effects Testing Guidelines (8) Result: slightly irritating, non-corrosive to rabbit skin 9.1.5 Eye Irritation (6) Species/strain: New Zealand White rabbits
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