10. ASSESSMENT OF ENVIRONMENTAL EFFECTS Tests were performed according to EC and OECD test guidelines (European Commission 1992) at Safepharm Laboratories Limited UK. These facilities comply with the OECD principles of good laboratory practice (GLP) and full test reports were submitted. All tests were performed on Alkane 2. 10.1 Summary of Effects on Biotic Systems Test Species Results Acute Toxicity Rainbow trout b > 1 000 mg/L Semi-static WAF a (Oncorhynchus NOEC 1 000 mg/L mykiss) ≥ 96 hour OECD TG 203 Acute Immobilisation Water Flea ELR50 c > 1 000 mg/L Static WAF a (Daphnia NOEC 1 000 mg/L magna) ≥ 48 hour OECD TG 202 Growth Inhibition Algae EbLR50 d > 1 000 mg/L Static (Selenastrum EµLR50 e > 1 000 mg/L capricornutum) 96 hour NOEC 1 000 mg/L ≥ OECD TG 201 WAF: water accommodated fraction - see comments in text below. LLR: lethal loading rate. ELR: effective loading rate. EbLR50: effective loading rate that reduced biomass by 50%. EµLR 50: effective loading rate that reduced specific growth rate by 50% (24-48 hours). f) NOEC - no observable effect concentration. Based on the results of rangefinding studies, limit tests were conducted for the definitive studies. The toxicity of Alkane 2 on fish, water flea and algae was examined using a Water Accommodated Fraction (WAF) with a loading rate of 1 000 mg/L. The test media was stirred for 20 hours, with the mixture then allowed to stand for 4 hours prior to the removal of the aqueous phase or WAF. Analysis of the WAF was carried out by Total Organic Carbon (TOC) analysis. The results showed the concentrations of the carbon in the test vessels to be around the limit of detection of the analytical method. All exposures are expressed in of the original concentration of the chemical in water at the preparation of the WAF (the loading rate), irrespective of the actual concentration of the chemical in water. During all testing, the WAF was observed to be a clear, colourless solution. 10.2 Fish Acute Toxicity Test (Safepharm Laboratories Limited 1995g)
10. ASSESSMENT OF ENVIRONMENTAL EFFECTS Tests were performed according to EC and OECD test guidelines (European Commission 1992) at Safepharm Laboratories Limited UK. These facilities comply with the OECD principles of good laboratory practice (GLP) and full test reports were submitted. All tests were performed on Alkane 2. 10.1 Summary of Effects on Biotic Systems Test Species Results Acute Toxicity Semi-static WAF a 96 hour OECD TG 203 Rainbow trout (Oncorhynchus mykiss) b > 1 000 mg/L NOEC ≥ 1 000 mg/L Acute Immobilisation Static WAF a 48 hour OECD TG 202 Water Flea (Daphnia magna) ELR50 c > 1 000 mg/L NOEC ≥ 1 000 mg/L Growth Inhibition Static 96 hour OECD TG 201 Algae (Selenastrum capricornutum) EbLR50 d > 1 000 mg/L EµLR50 e > 1 000 mg/L NOEC ≥ 1 000 mg/L WAF: water accommodated fraction - see comments in text below. LLR: lethal loading rate. ELR: effective loading rate. EbLR50: effective loading rate that reduced biomass by 50%. EµLR 50: effective loading rate that reduced specific growth rate by 50% (24-48 hours). f) NOEC - no observable effect concentration. Based on the results of rangefinding studies, limit tests were conducted for the definitive studies. The toxicity of Alkane 2 on fish, water flea and algae was examined using a Water Accommodated Fraction (WAF) with a loading rate of 1 000 mg/L. The test media was stirred for 20 hours, with the mixture then allowed to stand for 4 hours prior to the removal of the aqueous phase or WAF. Analysis of the WAF was carried out by Total Organic Carbon (TOC) analysis. The results showed the concentrations of the carbon in the test vessels to be around the limit of detection of the analytical method. All exposures are expressed in of the original concentration of the chemical in water at the preparation of the WAF (the loading rate), irrespective of the actual concentration of the chemical in water. During all testing, the WAF was observed to be a clear, colourless solution. 10.2 Fish Acute Toxicity Test (Safepharm Laboratories Limited 1995g)
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