(occlusive patch) and applied to the clipped surface of test animals as quickly as possible. Day 7 and Day 13 The induction procedure was repeated. Day 27 Challenge procedure: Chambers containing a challenge dose of 1.25% concentration of test substance were applied to the trunks of test animals and secured for 20 with elastic wrap and tape. Approximately 20 hours after chamber removal for challenge, the test sites were depiliated. Approximately six hours after chamber application, the elastic wrap, tape, and chambers were removed. The test sites were graded for irritation at approximately 24 and 48 hours following chamber application at induction and at approximately 24 and 48 hours following chamber removal at challenge US EPA Series 81 Test method: Following challenge with 1.25% w/v S10737.01, dermal Comment: scores of 0 (no reaction) to (slight patchy erythema) were noted in all test and challenge control animals. Group mean dermal scores were similar between the test and challenge control animals. The analogue chemical was non-sensitising to the skin of Result: guinea pigs. 9.2 A subchronic toxicity test with an analogue compound, xylene sulfonic acid sodium salt, in mice and rats. (Wheeler, 1980) Mouse/B6C3F1; rat/Fischer 344 Species/strain: 5 groups for both species Number/sex of animals: Diet ad libitum; Method of administration: diet levels 0.125%, 0.25%, 0.5%, 1.0% and 2% 91 days Dose/Study duration: Not stated Test method: Clinical observations and findings: Mice Mortality was limited to four mice, three males of the 0.5% level and one female of the lowest (0.125%) level. These deaths were not considered to be treatment related.
(occlusive patch) and applied to the clipped surface of test animals as quickly as possible. Day 7 and Day 13 The induction procedure was repeated. Challenge procedure: Day 27 Chambers containing a challenge dose of 1.25% concentration of test substance were applied to the trunks of test animals and secured for 20 with elastic wrap and tape. Approximately 20 hours after chamber removal for challenge, the test sites were depiliated. Approximately six hours after chamber application, the elastic wrap, tape, and chambers were removed. The test sites were graded for irritation at approximately 24 and 48 hours following chamber application at induction and at approximately 24 and 48 hours following chamber removal at challenge Test method: US EPA Series 81 Comment: Following challenge with 1.25% w/v S10737.01, dermal scores of 0 (no reaction) to (slight patchy erythema) were noted in all test and challenge control animals. Group mean dermal scores were similar between the test and challenge control animals. Result: The analogue chemical was non-sensitising to the skin of guinea pigs. 9.2 A subchronic toxicity test with an analogue compound, xylene sulfonic acid sodium salt, in mice and rats. (Wheeler, 1980) Species/strain: Mouse/B6C3F1; rat/Fischer 344 Number/sex of animals: 5 groups for both species Method of administration: Diet ad libitum; diet levels 0.125%, 0.25%, 0.5%, 1.0% and 2% Dose/Study duration: 91 days Test method: Not stated Clinical observations and findings: Mice Mortality was limited to four mice, three males of the 0.5% level and one female of the lowest (0.125%) level. These deaths were not considered to be treatment related.
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