TA98, 100, 1535, 1537; Strains: Salmonella typhimurium WP2 uvrA Escherischia coli Aroclor 1254-induced rat liver microsomal fraction S9 Metabolic activation: 312.5, 625, 1250, 2500, 5000g/plate Concentration range: OECD TG 471, 472 Test method: A finding toxicity test performed with and without Comment: microsomal activation determined a maximum test substance concentration without toxicity of 5000g/plate. In both initial and confirmatory experiments, no significant increase in the incidence of mutations was observed compared to negative controls. The notified chemical was non mutagenic the Result: conditions of the test. 9.3.2 Chromosomal Aberration Assay in Chinese Hamster Ovary Cells (Ogorek, 1992) Chinese hamster ovary CCL 61 Aroclor 1254-induced rat microsomal fraction S9 Metabolic activation system: Dosing schedule: Metabolic Experiment/ Test concentration (g/mL) Controls Activation Study Number -S9 Original study Treatment time = 18 hours Positive: Mitomycin C 5.1*, 10.2*, 20.3*, 40.6, 81.3, 162.5, 325, 650 g/ml Confirmation study Treatment time = 42 hours Negative: Ethanol 5.1*, 10.2*, 20.3*, 40.6, 81.3, vehicle 162.5, 325, 650 g/ml +S9 Original study Treatment time = 3 hours. Recovery Positive: time = 15 hours. Cyclophosphamide 5.1*, 10.2, 20.3, 40.6, 81.3, 162.5*, 325*, 650* g/ml Confirmation study Treatment time = 3 hours. Recovery Negative: Ethanol time = 15 or 39 hours. vehicle 5.1*, 10.2, 20.3, 40.6, 81.3, 162.5*, 325*, 650* g/ml * - cultures selected for metaphase analysis
Strains: Salmonella typhimurium TA98, 100, 1535, 1537; Escherischia coli WP2 uvrA Metabolic activation: Aroclor 1254-induced rat liver microsomal fraction S9 Concentration range: 312.5, 625, 1250, 2500, 5000g/plate Test method: OECD TG 471, 472 Comment: A finding toxicity test performed with and without microsomal activation determined a maximum test substance concentration without toxicity of 5000g/plate. In both initial and confirmatory experiments, no significant increase in the incidence of mutations was observed compared to negative controls. Result: The notified chemical was non mutagenic the conditions of the test. 9.3.2 Chromosomal Aberration Assay in Chinese Hamster Ovary Cells (Ogorek, 1992) Chinese hamster ovary CCL 61 Metabolic activation system: Aroclor 1254-induced rat microsomal fraction S9 Dosing schedule: Metabolic Activation Experiment/ Study Number Test concentration (g/mL) Controls -S9 Original study Confirmation study Treatment time = 18 hours 5.1*, 10.2*, 20.3*, 40.6, 81.3, 162.5, 325, 650 g/ml Treatment time = 42 hours 5.1*, 10.2*, 20.3*, 40.6, 81.3, 162.5, 325, 650 g/ml Positive: Mitomycin C Negative: Ethanol vehicle +S9 Original study Confirmation study Treatment time = 3 hours. Recovery time = 15 hours. 5.1*, 10.2, 20.3, 40.6, 81.3, 162.5*, 325*, 650* g/ml Treatment time = 3 hours. Recovery time = 15 or 39 hours. 5.1*, 10.2, 20.3, 40.6, 81.3, 162.5*, 325*, 650* g/ml Positive: Cyclophosphamide Negative: Ethanol vehicle * - cultures selected for metaphase analysis
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