demonstrated the test sensitivity. Negative control (vehicle) gave the expected response. The test substance was non clastogenic under the conditions Result: of the test and no increase in the frequency of micronucleated polychromatic erythrocytes occurred. 9.4 Overall Assessment of Toxicological Data Analogues of the notified chemical were of very low oral toxicity (LD50 > 5000 mg/kg, low acute dermal toxicity (LD50 > 4.64 mL/kg) and low acute inhalation toxicity (LC50 between 2.09 and 5.45 mg/L) in rats. Analogues of the notified chemical were slightly irritating to rabbit skin, not irritating to rabbit eyes and not skin sensitising in guinea pigs. The notified chemical exhibited diuretic effects in a 28-day oral repeated dose study in rats with a NOAEL of 50 mg/kg/day. Organ toxicity was limited to increased severity of hepatocyte microvacuolation at 500 mg/kg/day. A 90-day feeding study in rats was conducted on an analogue of the notified chemical. The NOAEL was 15 mg/kg/day on the basis of lower urine specific gravity in males, also an indicator of diuresis in the 28-day study with the notified chemical. Analogues of the notified chemical were not mutagenic in bacteria and were not clastogenic in mouse bone marrow cells in vivo. The test substance used to measure inhalation toxicity is determined to be a hazardous substance according to the NOHSC Approved Criteria for Classifying Hazardous Substances (NOHSC, 1999) and is assigned the risk phrase R20: Harmful by inhalation. Therefore, the notified chemical is assigned the risk phrase R20. 10. ASSESSMENT OF ENVIRONMENTAL EFFECTS A number of of the toxicity of the notified chemical or of a chemical analogue were provided, and the results of these test summarised below.
demonstrated the test sensitivity. Negative control (vehicle) gave the expected response. Result: The test substance was non clastogenic under the conditions of the test and no increase in the frequency of micronucleated polychromatic erythrocytes occurred. 9.4 Overall Assessment of Toxicological Data Analogues of the notified chemical were of very low oral toxicity (LD50 > 5000 mg/kg, low acute dermal toxicity (LD50 > 4.64 mL/kg) and low acute inhalation toxicity (LC50 between 2.09 and 5.45 mg/L) in rats. Analogues of the notified chemical were slightly irritating to rabbit skin, not irritating to rabbit eyes and not skin sensitising in guinea pigs. The notified chemical exhibited diuretic effects in a 28-day oral repeated dose study in rats with a NOAEL of 50 mg/kg/day. Organ toxicity was limited to increased severity of hepatocyte microvacuolation at 500 mg/kg/day. A 90-day feeding study in rats was conducted on an analogue of the notified chemical. The NOAEL was 15 mg/kg/day on the basis of lower urine specific gravity in males, also an indicator of diuresis in the 28-day study with the notified chemical. Analogues of the notified chemical were not mutagenic in bacteria and were not clastogenic in mouse bone marrow cells in vivo. The test substance used to measure inhalation toxicity is determined to be a hazardous substance according to the NOHSC Approved Criteria for Classifying Hazardous Substances (NOHSC, 1999) and is assigned the risk phrase R20: Harmful by inhalation. Therefore, the notified chemical is assigned the risk phrase R20. 10. ASSESSMENT OF ENVIRONMENTAL EFFECTS A number of of the toxicity of the notified chemical or of a chemical analogue were provided, and the results of these test summarised below.
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