and were all below the method detection limit. Accordingly, the bioconcentration factor was also below the limit of detection at <2.3 (Level 1) and <0.24 (Level 2). Therefore, the notified chemical is not expected to bioaccumulate in fish. This is not surprising, given the high water solubility of the notified chemical. 9. EVALUATION OF TOXICOLOGICAL DATA 9.1 Acute Toxicity Summary of the acute toxicity of the Dye for BJ Printer Ink 001 Reference Test Species Outcome acute oral toxicity rat LD50 >2000 mg/kg (Noakes, 1999c) acute dermal toxicity rat LD50 >2000 mg/kg (Noakes, skin irritation rabbit Non irritant (Noakes, 1999d) eye irritation rabbit Slight irritant (Noakes, 1999b) skin sensitisation guinea pig Non sensitiser (Noakes, 1999e) 9.1.1 Oral Toxicity (Noakes, Rat/Alpk:APfSD Species/strain: 5/sex Number/sex of animals: 14 days Observation period: A single oral dose of 2000 mg/kg notified chemical in Method of administration: deionised was given by gavage OECD TG 401 (limit test) Test method: None Mortality: Blue urine, and blue or blue/black faeces. Clinical observations: Fur and tail stained blue/black. One male and one female had pelvic dilation of the kidney. Morphological findings: Microscopic investigation was not conducted in the
and were all below the method detection limit. Accordingly, the bioconcentration factor was also below the limit of detection at <2.3 (Level 1) and <0.24 (Level 2). Therefore, the notified chemical is not expected to bioaccumulate in fish. This is not surprising, given the high water solubility of the notified chemical. 9. EVALUATION OF TOXICOLOGICAL DATA 9.1 Acute Toxicity Summary of the acute toxicity of the Dye for BJ Printer Ink 001 Test Species Outcome Reference acute oral toxicity rat LD50 >2000 mg/kg (Noakes, 1999c) acute dermal toxicity rat LD50 >2000 mg/kg (Noakes, skin irritation rabbit Non irritant (Noakes, 1999d) eye irritation rabbit Slight irritant (Noakes, 1999b) skin sensitisation guinea pig Non sensitiser (Noakes, 1999e) 9.1.1 Oral Toxicity (Noakes, Species/strain: Rat/Alpk:APfSD Number/sex of animals: 5/sex Observation period: 14 days Method of administration: A single oral dose of 2000 mg/kg notified chemical in deionised was given by gavage Test method: OECD TG 401 (limit test) Mortality: None Clinical observations: Blue urine, and blue or blue/black faeces. Fur and tail stained blue/black. Morphological findings: One male and one female had pelvic dilation of the kidney. Microscopic investigation was not conducted in the
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