OLOA 229 Test substance: Rabbit/New Zealand White Species/strain: 4 males Number/sex of animals: 14 days Observation period: A single, 24-hour occlusive application of 5 000 mg/kg of Method of administration: test substance to abraded (2 rabbits) and intact skin (2 rabbits). OECD TG 402 Test method: Nil Mortality: No signs of toxicity observed Clinical observations: No gross abnormalities detected Morphological findings: > 5000 mg/kg LD50: OLOA 229 was of low dermal toxicity to the rabbit. Result: 9.1.3.1 Skin Irritation (Cavalli MS 1970) OLOA 229 Test substance: Rabbit/New Zealand White Species/strain: 6 males Number/sex of animals: 3 days; observations done at 24 and 72 hours. Observation period: A single, 24-hour occlusive application of 0.5 mL of test Method of administration: substance to abraded and intact skin on each rabbit. Not stated but similar to OECD TG 404 Test method: Dermal scores for intact skin: Time Animal # treatment 1 2 3 4 5 6 (days) Erythema 1 a2 1 2 2 1 1
Test substance: OLOA 229 Species/strain: Rabbit/New Zealand White Number/sex of animals: 4 males Observation period: 14 days Method of administration: A single, 24-hour occlusive application of 5 000 mg/kg of test substance to abraded (2 rabbits) and intact skin (2 rabbits). Test method: OECD TG 402 Mortality: Nil Clinical observations: No signs of toxicity observed Morphological findings: No gross abnormalities detected LD50: > 5000 mg/kg Result: OLOA 229 was of low dermal toxicity to the rabbit. 9.1.3.1 Skin Irritation (Cavalli MS 1970) Test substance: OLOA 229 Species/strain: Rabbit/New Zealand White Number/sex of animals: 6 males Observation period: 3 days; observations done at 24 and 72 hours. Method of administration: A single, 24-hour occlusive application of 0.5 mL of test substance to abraded and intact skin on each rabbit. Test method: Not stated but similar to OECD TG 404 Dermal scores for intact skin: Time Animal # treatment (days) 1 2 3 4 5 6 Erythema 1 a2 1 2 2 1 1
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