Comments: Assays: (continued) Procedure Process Step Potential Hazards Initial Risk Control (Mitigation) Residual Level Risk Level Reagent Prepare BTS, matrix: Risk of chemical Medium Required: Low 70% formic acid solution exposure when Preparation for (Exposure to Follow written procedures. 70% ethanol (target aliquoting strong acids chemicals) Wear appropriate PPE: (no respirator tube extraction cleaning) and organic solvents necessary), lab coat, gloves, eye process 80% trifluoroacetic acid (Note: small volumes). protection. (target cleaning Follow Chemical Hygiene Plan. (for Mycobacteria acetonitrile Additional Protection Preferred: – and other high risk Perform work in a fume hood. pathogens) Wear additional chemical resistant gloves. Additional Protection - Alternative: If perform work outside of a fume hood, wear eye protection and work behind a safety shield or face shield. Comments: Concentration and Concentrate 3-6 ml Risk of aerosols, High Required: Low specimen by centrifugation. splashes, and splatter heat (MTBC) Follow written procedures. Pour off supernatant. when opening Post a sign on the door warning others when inactivation Add 1 ml water and vortex. pouring off Low working with AFB cultures. Transfer to 1.5 ml tube and supernatant, vortexing Perform all work-up of AFB isolates in a (NTM) (for Mycobacteria centrifuge again. and pipetting. II BSC using BSL-3 practices (controlled Pour off supernatant. and other high risk access to the area when working, Add 300 ul of water. decontamination of all waste, must wear pathogens) Make suspension from growth a solid front gown with cuffed sleeves, on solid media in 300 ul water gloves, eye protection and N-95 and vortex. respirator/PAPR). Heat for 30 minutes at 95-100C Use centrifuge w/ sealed carriers. degrees. Use microcentrifuge with screw cap/gasket. Comments:
Comments: Assays: (continued) Procedure Process Step Potential Hazards Initial Risk Level Control (Mitigation) Residual Risk Level Reagent Preparation for tube extraction process (for Mycobacteria and other high risk pathogens) Prepare BTS, matrix: 70% formic acid solution 70% ethanol (target cleaning) 80% trifluoroacetic acid (target cleaning acetonitrile Risk of chemical exposure when aliquoting strong acids and organic solvents (Note: small volumes). Medium (Exposure to chemicals) Required: Follow written procedures. Wear appropriate PPE: (no respirator necessary), lab coat, gloves, eye protection. Follow Chemical Hygiene Plan. Additional Protection – Preferred: Perform work in a fume hood. Wear additional chemical resistant gloves. Additional Protection - Alternative: If perform work outside of a fume hood, wear eye protection and work behind a safety shield or face shield. Low Comments: Concentration and heat inactivation (for Mycobacteria and other high risk pathogens) Concentrate 3-6 ml specimen by centrifugation. Pour off supernatant. Add 1 ml water and vortex. Transfer to 1.5 ml tube and centrifuge again. Pour off supernatant. Add 300 ul of water. Make suspension from growth on solid media in 300 ul water and vortex. Heat for 30 minutes at 95-100C degrees. Risk of aerosols, splashes, and splatter when opening pouring off supernatant, vortexing and pipetting. High (MTBC) Low (NTM) Required: Follow written procedures. Post a sign on the door warning others when working with AFB cultures. Perform all work-up of AFB isolates in a II BSC using BSL-3 practices (controlled access to the area when working, decontamination of all waste, must wear a solid front gown with cuffed sleeves, gloves, eye protection and N-95 respirator/PAPR). Use centrifuge w/ sealed carriers. Use microcentrifuge with screw cap/gasket. Low Comments:
171025-Final-Revised-Risk-Assessment-Tool.docx