concentration, humidity level, temperature, if applicable: Additional strain / List sup. (picklist Picklist values: For robust study summaries, indicate additional cell type with remarks) - DNA polymerase A deficient strain characteristics (e.g. characteristics - acetyltransferase proficient only if necessary to characterise the Display: Basic - acetyltransferase deficient test system. Otherwise, leave this subfield empty. - nitroreductase deficient - p53 proficiency for cell - not applicable - other: - not specified Species / strain Block of fields (repeatable) End Cytokinesis Text (2,000 If a cytokinesis blocking substance (e.g. cytoB) (if used) char.) was used, indicate its identity and its concentration and duration of cell exposure. Display: Metabolic List (picklist) Picklist values: Indicate whether metabolic activation was applied activation - with or not. Select 'not applicable' for mammalian cell Display: Basic - with and without lines when no exogenous metabolic system is - without required. - not applicable - not specified Metabolic Text template Freetext template: For robust study summaries, specify metabolic activation system Type and composition of metabolic activation activation system, if any. Indicate the type and Display: Basic system: composition of and acceptability criteria for the - source of S9 metabolic activation system used. Alternatively or - method of preparation of S9 mix in addition refer to appropriate table(s), which can - concentration or volume of S9 mix and S9 in the be uploaded in the rich text field other “Any final culture medium information on materials and methods incl. - quality controls of S9 (e.g., enzymatic activity, tables”. Use predefined table or adapt table(s) sterility, metabolic capability) from study report. Use table numbers in the sequence in which you refer to them in the Remarks text (e.g. '... see Table 1'). Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD
concentration, humidity level, temperature, if applicable: Additional strain / cell type characteristics List sup. (picklist with remarks) Display: Basic Picklist values: - DNA polymerase A deficient - acetyltransferase proficient - acetyltransferase deficient - nitroreductase deficient - p53 proficiency for cell - not applicable - other: - not specified For robust study summaries, indicate additional strain characteristics (e.g. only if necessary to characterise the test system. Otherwise, leave this subfield empty. Species / strain Block of fields (repeatable) End Cytokinesis (if used) Text (2,000 char.) Display: If a cytokinesis blocking substance (e.g. cytoB) was used, indicate its identity and its concentration and duration of cell exposure. Metabolic activation List (picklist) Display: Basic Picklist values: - with - with and without - without - not applicable - not specified Indicate whether metabolic activation was applied or not. Select 'not applicable' for mammalian cell lines when no exogenous metabolic system is required. Metabolic activation system Text template Display: Basic Freetext template: Type and composition of metabolic activation system: - source of S9 - method of preparation of S9 mix - concentration or volume of S9 mix and S9 in the final culture medium - quality controls of S9 (e.g., enzymatic activity, sterility, metabolic capability) For robust study summaries, specify metabolic activation system, if any. Indicate the type and composition of and acceptability criteria for the metabolic activation system used. Alternatively or in addition refer to appropriate table(s), which can be uploaded in the rich text field “Any other information on materials and methods incl. tables”. Use predefined table or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the Remarks text (e.g. '... see Table 1'). Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD
OHT%2070%20-%20ENDPOINT_STUDY_RECORD.GeneticToxicityVitro%20-version%207.0%20-Dec%202017.docx