Test animals Control animals Challenge Concentration 24 hours* 48 hours* 24 hours 48 Challenge 1 0/10** 0/10 0/4 0/4 (33%) Challenge 2 0/10 0/10 0/4 0/4 (33%) Challenge 3 0/10 0/10 0/10 0/10 (33%) * time patch removal ** number of animals exhibiting erythema In the first challenge, the response was similar to that Comment: observed in treated controls. In the second challenge minimal erythema was observed and, under the conditions of the test, is not considered a positive response. SS was not sensitising to the skin of guinea pigs. Result: Skin Sensitisation test with SS (SEAC Toxicology, 1983) Guinea pigs/Albino Dunkin/Hartley Species/strain: 10 (6 males, 4 females) Number of animals: Induction procedure: Test Three pairs of intradermal injections (0.1 mL) were made on day 0 the shoulder region: FCA diluted 1:1 with physiological saline SS, 0.5% in physiological saline SS, 0.5% in a 1:1 mixture of FCA and saline A 2x4 cm filter patch was saturated with 25% SS and day 7 placed over the shaved area and covered by impermeable polythene adhesive tape. Control group: Treated controls: During the induction phase control animals (4/same sex) were treated in the same way as test animals except that the test substance was omitted from the intradermal injections and topical applications. Untreated controls: At challenge in the test, 4 previously untreated animals of the same sex and weighing approximately the same as the test animals at that challenge are treated in exactly the same way as the test animals. Challenge procedure:
Challenge Test animals Control animals Concentration 24 hours* 48 hours* 24 hours 48 Challenge 1 (33%) 0/10** 0/10 0/4 0/4 Challenge 2 (33%) 0/10 0/10 0/4 0/4 Challenge 3 (33%) 0/10 0/10 0/10 0/10 * time patch removal ** number of animals exhibiting erythema Comment: In the first challenge, the response was similar to that observed in treated controls. In the second challenge minimal erythema was observed and, under the conditions of the test, is not considered a positive response. Result: SS was not sensitising to the skin of guinea pigs. Skin Sensitisation test with SS (SEAC Toxicology, 1983) Species/strain: Guinea pigs/Albino Dunkin/Hartley Number of animals: 10 (6 males, 4 females) Induction procedure: Test day 0 day 7 Three pairs of intradermal injections (0.1 mL) were made on the shoulder region: FCA diluted 1:1 with physiological saline SS, 0.5% in physiological saline SS, 0.5% in a 1:1 mixture of FCA and saline A 2x4 cm filter patch was saturated with 25% SS and placed over the shaved area and covered by impermeable polythene adhesive tape. Control group: Treated controls: During the induction phase control animals (4/same sex) were treated in the same way as test animals except that the test substance was omitted from the intradermal injections and topical applications. Untreated controls: At challenge in the test, 4 previously untreated animals of the same sex and weighing approximately the same as the test animals at that challenge are treated in exactly the same way as the test animals. Challenge procedure:
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