Table 2. SAMPLE DESTINATIONS & HANDLING INSTRUCTIONS TEST TUBE LAB SPECIMEN SPECIMEN HANDLING/STORAGE PRE-ANALYSIS SPECIMEN DESTINATION HANDLING/ HANDLING/STORAGE TESTING POST-ANALYSIS INSTRUCTIONS HIV test EDTA Haematology Sent for Rapid Store whole blood at 2-8 o C up to 3 days. If testing delayed Store plasma at 2-8 o C up to double ELISA >3 days, centrifuge and remove plasma. Store plasma at 2-8 3 days; >3 days freeze o C up to 3 days; >3 days freeze plasma at -20o C. plasma at -20o C.. CD4 count EDTA Blood Haematology Done on HIV Pos Store at RT up to 24 hrs post collection. Test must be Test must be performed patients only. performed within that time period. within 24 hrs. Specimen not suitable >24 hrs post collection. HIV EDTA Blood Haematology Done on HIV Pos Centrifuge blood at 3500 rpm/1500xg for 10 min, remove Store remaining plasma Quantitation patients only. plasma and store 2 aliquots of 500 ul each at -80 C. aliquots at -80 C o o Creatinine Clotted Blood Chemistry Clotted sample only Serum or plasma (creatinine in serum is stable for 24 hours Archive serum into 2ml at 2–8ºC) cryovials at -80 C. o Hepatitis B Serum/plasma Chemistry Clotted sample only Centrifuge at 3500 rpm/1500xg for 10 min, remove serum, Archive serum into 2ml surface antigen freeze/archive serum into 2ml cryovials at -80 C. cryovials at -80 C. o o
Table 2. SAMPLE DESTINATIONS & HANDLING INSTRUCTIONS TEST TUBE LAB DESTINATION SPECIMEN HANDLING/ TESTING INSTRUCTIONS SPECIMEN HANDLING/STORAGE PRE-ANALYSIS SPECIMEN HANDLING/STORAGE POST-ANALYSIS HIV test EDTA Haematology Sent for Rapid double ELISA Store whole blood at 2-8 o C up to 3 days. If testing delayed >3 days, centrifuge and remove plasma. Store plasma at 2-8 o C up to 3 days; >3 days freeze plasma at -20o C. Store plasma at 2-8 o C up to 3 days; >3 days freeze plasma at -20o C.. CD4 count EDTA Blood Haematology Done on HIV Pos patients only. Store at RT up to 24 hrs post collection. Test must be performed within that time period. Test must be performed within 24 hrs. Specimen not suitable >24 hrs post collection. HIV Quantitation EDTA Blood Haematology Done on HIV Pos patients only. Centrifuge blood at 3500 rpm/1500xg for 10 min, remove plasma and store 2 aliquots of 500 ul each at -80 o C. Store remaining plasma aliquots at -80 o C Creatinine Clotted Blood Chemistry Clotted sample only Serum or plasma (creatinine in serum is stable for 24 hours at 2–8ºC) Archive serum into 2ml cryovials at -80 o C. Hepatitis B surface antigen Serum/plasma Chemistry Clotted sample only Centrifuge at 3500 rpm/1500xg for 10 min, remove serum, freeze/archive serum into 2ml cryovials at -80 o C. Archive serum into 2ml cryovials at -80 o C.
NNACA_SOP_302.docx