In the E. coli reversion test, no increase in the number of Comment: revertant colonies was observed at any concentration, compared with the negative control. In the DNA repair test, no zone of inhibition was observed for the test substance. The positive controls behaved appropriately in both tests. The notified chemical was non mutagenic under the Result: conditions of the test. 9.3.3 Stamen Hair System (Mičieta, 1990) Tradescantia 4430 Strains: Tradescantia 0.2% (w/v) test substance in DMSO 0.2% (v/v), Tween 80 Concentration range: (v/v) 0.2% in water. The tester plant species is heterozygous at a flower colour Test method: locus. A blue phenotype is a product of a dominant blue allele, and a pink phenotype is controlled by a recessive pink allele unmasked by mutation or deletion of the blue allele. For each treatment, entire inflorescens are immersed in test solution, washed and planted in Hoagland’s nutrient solution. For each treatment, 10 flowers/3000 stamen hairs were scored daily between days 4 and 12 after commencement of treatment. The test substance was insoluble in water and of low Comment: solubility in organic solvents. The notified chemical was non mutagenic under the Result: conditions of the test. 9.3.4 In vivo Cytogenetic Test Test (Murín, 1990) – Strain: sativa L. cv. Fatima 0.2, 0.1 or 0.05% (w/v) test substance in 0.4% (v/v) acetone, Concentration range: Tween 80 (one drop) in water. Seeds were immersed in test substance solutions for 24 Test method: hours and then germinated in petri dishes on filter paper wetted by similar solutions. After 72 hours, root tip samples were evaluated for structural and numerical chromosome mutations. The test substance was insoluble in water and of low Comment: solubility in organic solvents. No chromosome mutations were observed for any concentration of test substance. The positive and negative controls behaved appropriately.
Comment: In the E. coli reversion test, no increase in the number of revertant colonies was observed at any concentration, compared with the negative control. In the DNA repair test, no zone of inhibition was observed for the test substance. The positive controls behaved appropriately in both tests. Result: The notified chemical was non mutagenic under the conditions of the test. 9.3.3 Tradescantia Stamen Hair System (Mičieta, 1990) Strains: Tradescantia 4430 Concentration range: 0.2% (w/v) test substance in DMSO 0.2% (v/v), Tween 80 (v/v) 0.2% in water. Test method: The tester plant species is heterozygous at a flower colour locus. A blue phenotype is a product of a dominant blue allele, and a pink phenotype is controlled by a recessive pink allele unmasked by mutation or deletion of the blue allele. For each treatment, entire inflorescens are immersed in test solution, washed and planted in Hoagland’s nutrient solution. For each treatment, 10 flowers/3000 stamen hairs were scored daily between days 4 and 12 after commencement of treatment. Comment: The test substance was insoluble in water and of low solubility in organic solvents. Result: The notified chemical was non mutagenic under the conditions of the test. 9.3.4 In vivo Cytogenetic Test – Test (Murín, 1990) Strain: sativa L. cv. Fatima Concentration range: 0.2, 0.1 or 0.05% (w/v) test substance in 0.4% (v/v) acetone, Tween 80 (one drop) in water. Test method: Seeds were immersed in test substance solutions for 24 hours and then germinated in petri dishes on filter paper wetted by similar solutions. After 72 hours, root tip samples were evaluated for structural and numerical chromosome mutations. Comment: The test substance was insoluble in water and of low solubility in organic solvents. No chromosome mutations were observed for any concentration of test substance. The positive and negative controls behaved appropriately.
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