Performs: Examine media for Risk of aerosol production Moderate Required: Low growth. (See Table 1 for aerosol Viral culture ID Follow written procedures. Work up cultures as per generating activities) Wear PPE: lab coat, gloves and additional High (MERS, laboratory procedure Risk of contamination by eye protection when there is a risk of a SARS, Avian based on specimen touching growth splash or spray. influenza, etc.) source. Risk of spill or splash Do not touch your face, eyes, nose, or when handling liquid mouth without washing your hands List your test specific process media first. steps here. Perform all work with open inoculated media for viral cultures in a Class II BSC. Perform all work with suspected or known highly pathogen viruses in a Class II BSC using BSL-3 practices (controlled access to the area when working, decontamination of all waste, must wear a solid front gown with cuffed sleeves, gloves and N-95 respirator/PAPR). Work with slides for immunofluorescence staining within a Class II BSC until they are fixed. Once slides are fixed, they may be stained outside the BSC on the bench top. Comments: Culture Work-up: (continued) Procedure Process Step Potential Hazards Initial Risk Control (Mitigation) Residual Level Risk Level Isolate/ Store completed culture Low Required: Low Leak or spill during media at appropriate storage Specimen Follow written procedure. temperature for Wear PPE: lab coat, gloves and additional eye Retention appropriate time per protection when there is a risk of a splash procedure. or spray. Store isolates in racks, or Wear additional freezer gloves if storing in boxes, or in sealed ultra low (-70○ C) freezer. biohazard bags to prevent leaks and spills.
Performs: Viral culture ID Examine media for growth. Work up cultures as per laboratory procedure based on specimen source. List your test specific process steps here. Risk of aerosol production (See Table 1 for aerosol generating activities) Risk of contamination by touching growth Risk of spill or splash when handling liquid media Moderate High (MERS, SARS, Avian influenza, etc.) Required: Follow written procedures. Wear PPE: lab coat, gloves and additional eye protection when there is a risk of a splash or spray. Do not touch your face, eyes, nose, or mouth without washing your hands first. Perform all work with open inoculated media for viral cultures in a Class II BSC. Perform all work with suspected or known highly pathogen viruses in a Class II BSC using BSL-3 practices (controlled access to the area when working, decontamination of all waste, must wear a solid front gown with cuffed sleeves, gloves and N-95 respirator/PAPR). Work with slides for immunofluorescence staining within a Class II BSC until they are fixed. Once slides are fixed, they may be stained outside the BSC on the bench top. Low Comments: Culture Work-up: (continued) Procedure Process Step Potential Hazards Initial Risk Level Control (Mitigation) Residual Risk Level Isolate/ Specimen Retention Store completed culture media at appropriate temperature for appropriate time per procedure. Store isolates in racks, or boxes, or in sealed biohazard bags to prevent leaks and spills. Leak or spill during storage Low Required: Follow written procedure. Wear PPE: lab coat, gloves and additional eye protection when there is a risk of a splash or spray. Wear additional freezer gloves if storing in ultra low (-70○ C) freezer. Low
171025-Final-Revised-Risk-Assessment-Tool.docx