Alkane 4 Result: was of very low acute inhalation toxicity to the rat. 9.1.4 Skin Irritation (Safepharm Laboratories Limited 1995b) Rabbit/New Zealand white Species/strain: 4 females, 2 males Number/sex of animals: 3 days Observation period: A single 4 semi occluded application of 0.5 mL of test Method of administration: substance to intact skin. OECD TG 404; EC Method B4. Test method: Grade 1 erythema (very slight erythema) was observed at Dermal response: two treated sites 30 minutes after patch removal and persisted at one treated site at the 24 observation. All treated sites appeared normal at the 48 hour observation time. Erythema/Eschar formation: 0.06; 24, 48 & 72 hour mean Oedema: 0.0. score: Alkane 4 was very slightly irritating to rabbit skin. Result: 9.1.5 Eye Irritation (Safepharm Laboratories Limited 1995o) Rabbit/New Zealand White Species/strain: 5 males, 4 females Number/sex of animals: 1, 24, 48 and 72 hours post instillation Observation period: A single instillation of 0.1 mL of the neat test substance into Method of administration, the conjunctival sac of the right eye of 6 rabbits. The left eye Unirrigated eyes: served as the control. A single instillation of 0.1 mL of the neat test substance into Method of administration, the conjunctival sac of the eye of 3 rabbits; after 30 seconds Irrigated eyes: the eye was gently irrigated with 100mL of lukewarm water for one minute. The untreated eye served as the control.
Result: Alkane 4 was of very low acute inhalation toxicity to the rat. 9.1.4 Skin Irritation (Safepharm Laboratories Limited 1995b) Species/strain: Rabbit/New Zealand white Number/sex of animals: 4 females, 2 males Observation period: 3 days Method of administration: A single 4 semi occluded application of 0.5 mL of test substance to intact skin. Test method: OECD TG 404; EC Method B4. Dermal response: Grade 1 erythema (very slight erythema) was observed at two treated sites 30 minutes after patch removal and persisted at one treated site at the 24 observation. All treated sites appeared normal at the 48 hour observation time. 24, 48 & 72 hour mean score: Erythema/Eschar formation: 0.06; Oedema: 0.0. Result: Alkane 4 was very slightly irritating to rabbit skin. 9.1.5 Eye Irritation (Safepharm Laboratories Limited 1995o) Species/strain: Rabbit/New Zealand White Number/sex of animals: 5 males, 4 females Observation period: 1, 24, 48 and 72 hours post instillation Method of administration, Unirrigated eyes: A single instillation of 0.1 mL of the neat test substance into the conjunctival sac of the right eye of 6 rabbits. The left eye served as the control. Method of administration, Irrigated eyes: A single instillation of 0.1 mL of the neat test substance into the conjunctival sac of the eye of 3 rabbits; after 30 seconds the eye was gently irrigated with 100mL of lukewarm water for one minute. The untreated eye served as the control.
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