9.1.5 Eye Irritation (Braun, 1996b) rabbit/New Zealand Chbb:NZW(SPF) Species/strain: 1 male, 2 females Number/sex of animals: 21 days Observation period: 0.1 g the test substance was installed into one eye Method of of the rabbits administration: conjunctiva: chemosis observed in all animals up Draize scores: to 24 hours, all other readings zero; cornea and iris: all scores zero. staining in conjunctivae (including nictitating Other relevant observations: membrane) and sclera was noted in all animals up to day 21, light violet staining of the cornea was noted in 2 of the 3 animals but was reversible by day 7. OECD TG 405 (Organisation for Economic Test method: Co- operation and Development, 1995-1996) FAT 40548/A caused damage to the eyes of rabbits Result: based on its persistent colouration effects. The substance was a slight irritant to rabbit eyes. Skin Sensitisation (Arcelin, 1996c) guinea pig/Ibm: GOHI; SPF Species/strain: 20 (test), 10 (control) Number of animals: day 1-intradermal induction: 3 pairs of injections Induction procedure: (0.1 mL) were made: saline:Freund’s Complete Adjuvant (FCA) (1:1) (v/v) the notified chemical in water (5%, w/v) the notified chemical (5%, w/v) in FCA/saline mixture (1:1)
9.1.5 Eye Irritation (Braun, 1996b) Species/strain: Number/sex of animals: Observation period: Method of administration: rabbit/New Zealand Chbb:NZW(SPF) 1 male, 2 females 21 days 0.1 g the test substance was installed into one eye of the rabbits Draize scores: conjunctiva: chemosis observed in all animals up to 24 hours, all other readings zero; cornea and iris: all scores zero. Other relevant observations: staining in conjunctivae (including nictitating membrane) and sclera was noted in all animals up to day 21, light violet staining of the cornea was noted in 2 of the 3 animals but was reversible by day 7. Test method: OECD TG 405 (Organisation for Economic Co- operation and Development, 1995-1996) Result: FAT 40548/A caused damage to the eyes of rabbits based on its persistent colouration effects. The substance was a slight irritant to rabbit eyes. Skin Sensitisation (Arcelin, 1996c) Species/strain: guinea pig/Ibm: GOHI; SPF Number of animals: 20 (test), 10 (control) Induction procedure: day 1-intradermal induction: 3 pairs of injections (0.1 mL) were made: saline:Freund’s Complete Adjuvant (FCA) (1:1) (v/v) the notified chemical in water (5%, w/v) the notified chemical (5%, w/v) in FCA/saline mixture (1:1)
NA632FR.docx