10 000 mg/kg (dose volume 16 mL/kg) of test substance Method of administration: applied to an intact and abraded area of skin and held under semi occlusive dressing for 24 hours;. 14 days Observation period: US Federal Hazardous Substances Labelling Act. Test method: Nil. Mortality: Skin became very taut, dry and scaly, with no regrowth of Clinical observations: hair at the treatment site No abnormalities detected at necropsy. Morphological findings: > 10 000 mg/kg LD50: C12-C16 Olefin was of low dermal toxicity in rats. Result: 9.1.2.2 Acute Dermal Toxicity of C16 Alpha Olefin (Rinehart 1967) C16 Alpha Olefin (98.5% olefin,1.5% saturates) Test Substance: Rabbit/New Zealand Species/strain: 4 males Number/sex of animals: 10 000 mg/kg (dose volume 16 mL/kg) of test substance Method of administration: applied to an intact and abraded area of skin and held semi occlusive dressing for 24 hours. 14 days Observation period: US Federal Hazardous Substances Labelling Act Test method: One death due to broken neck Mortality: Skin became very taut, dry and with no regrowth of Clinical observations: hair at the treatment site. No abnormalities detected at necropsy. Morphological findings: > 10 000 mg/kg LD50: C16 Alpha Olefin was of low dermal toxicity in rats. Result: 9.1.2.3 Dermal Toxicity of C16 Isomerised Olefin (Stillmeadow Inc 1993) C16 Isomerised Olefin Test Substance:
Method of administration: 10 000 mg/kg (dose volume 16 mL/kg) of test substance applied to an intact and abraded area of skin and held under semi occlusive dressing for 24 hours;. Observation period: 14 days Test method: US Federal Hazardous Substances Labelling Act. Mortality: Nil. Clinical observations: Skin became very taut, dry and scaly, with no regrowth of hair at the treatment site Morphological findings: No abnormalities detected at necropsy. LD50: > 10 000 mg/kg Result: C12-C16 Olefin was of low dermal toxicity in rats. 9.1.2.2 Acute Dermal Toxicity of C16 Alpha Olefin (Rinehart 1967) Test Substance: C16 Alpha Olefin (98.5% olefin,1.5% saturates) Species/strain: Rabbit/New Zealand Number/sex of animals: 4 males Method of administration: 10 000 mg/kg (dose volume 16 mL/kg) of test substance applied to an intact and abraded area of skin and held semi occlusive dressing for 24 hours. Observation period: 14 days Test method: US Federal Hazardous Substances Labelling Act Mortality: One death due to broken neck Clinical observations: Skin became very taut, dry and with no regrowth of hair at the treatment site. Morphological findings: No abnormalities detected at necropsy. LD50: > 10 000 mg/kg Result: C16 Alpha Olefin was of low dermal toxicity in rats. 9.1.2.3 Dermal Toxicity of C16 Isomerised Olefin (Stillmeadow Inc 1993) Test Substance: C16 Isomerised Olefin
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