The gene expression was measured with SYBR green quantitative PCR (Takara RR820A). Data analysis Human lung adenocarcinoma and TCGA breast cancer data were obtained from Oncomine database (https://www.oncomine.org). Figure S1. Inhibition of apoptosis cannot suppress induced cell invasion. lgl-Ras (A-D) GFP-labeled clones of cells with indicated genotypes are created in the developing eye-antennal discs. induced tumor invasion into the VNC RasV12/lgl-/- (A’, n=27) was not affected by expressing YkiS168A (B’, n=10), DIAP1 (C’, n=23), or p35 (D’, n=25). (E) A quantification of tumor invasion phenotype in A’-D’ was shown. (F-G) Caspase 3 activation in tumors (F’) was not significantly affected by lgl-Ras dMyc/dMax expression (G’). Figure S2. Myc expression does not increase induced apoptosis. ptc>scrib-IR Fluorescence micrographs of wing pouch regions are shown. Anterior is to the left and dorsal up in all figures. Loss of induced caspase activation (A’) remained scirb unaffected by the expression of dMax, dMyc, dMyc/dMax, cMyc, or cMyc/dMax Crosses were done at 29 ºC. Figure S3. Inhibition of apoptosis cannot suppress induced cell ptc>scirb-IR invasion. Loss of induced cell invasion (A) could not be suppressed by the expression of scirb DIAP1 (B). Crosses were done at 29 ºC. Figure S4. Myc suppresses ectopic Hep induced cell invasion.
The gene expression was measured with SYBR green quantitative PCR (Takara RR820A). Data analysis Human lung adenocarcinoma and TCGA breast cancer data were obtained from Oncomine database (https://www.oncomine.org). Figure S1. Inhibition of apoptosis cannot suppress lgl-Ras induced cell invasion. (A-D) GFP-labeled clones of cells with indicated genotypes are created in the developing eye-antennal discs. RasV12/lgl-/- induced tumor invasion into the VNC (A’, n=27) was not affected by expressing YkiS168A (B’, n=10), DIAP1 (C’, n=23), or p35 (D’, n=25). (E) A quantification of tumor invasion phenotype in A’-D’ was shown. (F-G) Caspase 3 activation in lgl-Ras tumors (F’) was not significantly affected by dMyc/dMax expression (G’). Figure S2. Myc expression does not increase ptc>scrib-IR induced apoptosis. Fluorescence micrographs of wing pouch regions are shown. Anterior is to the left and dorsal up in all figures. Loss of scirb induced caspase activation (A’) remained unaffected by the expression of dMax, dMyc, dMyc/dMax, cMyc, or cMyc/dMax Crosses were done at 29 ºC. Figure S3. Inhibition of apoptosis cannot suppress ptc>scirb-IR induced cell invasion. Loss of scirb induced cell invasion (A) could not be suppressed by the expression of DIAP1 (B). Crosses were done at 29 ºC. Figure S4. Myc suppresses ectopic Hep induced cell invasion.
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