Shimadzu Spectrofluorophotometer Quick User-Guide Beth Donovan 3/4/2014 Turn on the instrument and lamp by flipping the power switch on right side of instrument. Allow the lamp to warm up for about 20 minutes for best results. Click the icon on the desktop to open the software. “Fluorometer” Allow the instrument to complete its start-up checks. All green passed marks should evolve. Configure Parameters and adjust values as desired: → Spectrum Type: select •Emission Excitation wavelength Start and End wavelength emission Scanning Speed: select Medium Note: Do not use the AutoZero icon for best results. Place sample cuvette into instrument and click Start. Enter a file name (8 character maximum). Process data as desired: Austoscale: Right click on spectrum Radar Both Axes → → Peaks: Click manipulate Point pick. Type points in boxes, or drag → the cursor. Click OK, and a new window opens allowing you to print peak info: Output Print Table → Print Spectrum: Click Presentation Plot Print → →
Shimadzu Spectrofluorophotometer Quick User-Guide Beth Donovan 3/4/2014 Turn on the instrument and lamp by flipping the power switch on right side of instrument. Allow the lamp to warm up for about 20 minutes for best results. Click the “Fluorometer” icon on the desktop to open the software. Allow the instrument to complete its start-up checks. All green passed marks should evolve. Configure → Parameters and adjust values as desired: Spectrum Type: select •Emission Excitation wavelength Start and End wavelength emission Scanning Speed: select Medium Note: Do not use the AutoZero icon for best results. Place sample cuvette into instrument and click Start. Enter a file name (8 character maximum). Process data as desired: Austoscale: Right click on spectrum → Radar → Both Axes Peaks: Click manipulate → Point pick. Type points in boxes, or drag the cursor. Click OK, and a new window opens allowing you to print peak info: Output → Print Table Print Spectrum: Click Presentation → Plot → Print
optical-spectroscopy-spectrofluorophotometer-guide.docx