9.1.6.3 OLOA 2000 (15% OLOA 224) (Hill Top Research, 1996) Guinea pig/Dunkin-Hartley. Species/strain: 10 control/ 20 test. Number of animals: Induction procedure: test group: 3 treatments at 0.3 mL of the undiluted test substance placed in a 25 mm intervals of 7 Hill Top Chamber applied to the left shoulder and occluded days for 6 hours. control group: As for test group using mineral oil. Challenge procedure: Approximately 2 Same exposure procedure as for induction phase but at a weeks after last different skin site; 1% in mineral oil. induction exposure OECD TG 406 Test method: Challenge outcome: Test animals Control animals Challenge concentration 24 hours* 48 24 hours 48 hours 1% **0/20 2/20 0/10 0/10 * time after patch removal ** number of animals exhibiting positive response (ie Draize score 1) OLOA 2000 was sensitising to the skin of guinea pigs. Result: 9.1.6.4 XA 14328 (5.9% OLOA 224) (Hill Top Biolabs, 1995) Guinea pig/Dunkin-Hartley. Species/strain: 10 control/ 20 test. Number of animals: Induction procedure: test group: 3 treatments at 0.3 mL of the undiluted test substance placed in a 25 mm intervals of 7 Hill Top Chamber applied to the left shoulder and occluded days for 6 control group: As for test group using mineral oil. Challenge procedure:
9.1.6.3 OLOA 2000 (15% OLOA 224) (Hill Top Research, 1996) Species/strain: Guinea pig/Dunkin-Hartley. Number of animals: 10 control/ 20 test. Induction procedure: test group: 3 treatments at intervals of 7 days 0.3 mL of the undiluted test substance placed in a 25 mm Hill Top Chamber applied to the left shoulder and occluded for 6 hours. control group: As for test group using mineral oil. Challenge procedure: Approximately 2 weeks after last induction exposure Same exposure procedure as for induction phase but at a different skin site; 1% in mineral oil. Test method: OECD TG 406 Challenge outcome: Challenge Test animals Control animals concentration 24 hours* 48 24 hours 48 hours 1% **0/20 2/20 0/10 0/10 * time after patch removal ** number of animals exhibiting positive response (ie Draize score 1) Result: OLOA 2000 was sensitising to the skin of guinea pigs. 9.1.6.4 XA 14328 (5.9% OLOA 224) (Hill Top Biolabs, 1995) Species/strain: Guinea pig/Dunkin-Hartley. Number of animals: 10 control/ 20 test. Induction procedure: test group: 3 treatments at intervals of 7 days 0.3 mL of the undiluted test substance placed in a 25 mm Hill Top Chamber applied to the left shoulder and occluded for 6 control group: As for test group using mineral oil. Challenge procedure:
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