Click all” and configure the display modes as follows: “Hide and Regulation” “Expression Base Position: full Under and Gene Prediction Tracks” “Genes Reconciled Gene Models: pack D. mel Transcripts: pack Under Seq Tracks “RNA RNA-Seq Alignment Summary: show RNA-Seq TopHat: pack Under and Regulation” “Expression RNA PolII Peaks: pack RNA PolII Enrichment: full Under Genomics” “Comparative Conservation: pack Click on any of the refresh buttons. Finally, to zoom in to the region surrounding the TSS of the gene, type myo into the text box and click the button. position or search terms” “enter “go” Figure 8: Genome browser window of the region surrounding the TSS of the myo gene in D. biarmipes showing the tracks necessary for identifying a TSS search region when BLASTn to locate the 1st transcribed D. melanogaster exon. Lines are drawn from the top to ten landmarks that may or may not be used to define a TSS search region. Q8. In Figure 8, numbered lines are drawn to ten locations in the vicinity of the TSS of the myo D. biarmipes. gene on contig40 in List which of the lines point to landmarks that are supported by evidence and which lines point to positions that are not supported by evidence. Explain your decision in each case.
Click “Hide all” and configure the display modes as follows: “Expression and Regulation” Base Position: full Under “Genes and Gene Prediction Tracks” Reconciled Gene Models: pack D. mel Transcripts: pack Under “RNA Seq Tracks RNA-Seq Alignment Summary: show RNA-Seq TopHat: pack Under “Expression and Regulation” RNA PolII Peaks: pack RNA PolII Enrichment: full Under “Comparative Genomics” Conservation: pack Click on any of the refresh buttons. Finally, to zoom in to the region surrounding the TSS of the myo gene, type into the “enter position or search terms” text box and click the “go” button. Figure 8: Genome browser window of the region surrounding the TSS of the myo gene in D. biarmipes showing the tracks necessary for identifying a TSS search region when BLASTn to locate the 1st transcribed D. melanogaster exon. Lines are drawn from the top to ten landmarks that may or may not be used to define a TSS search region. Q8. In Figure 8, numbered lines are drawn to ten locations in the vicinity of the TSS of the myo gene on contig40 in D. biarmipes. List which of the lines point to landmarks that are supported by evidence and which lines point to positions that are not supported by evidence. Explain your decision in each case.
Module_TSS4_Advanced.docx