10% rat S9 fraction (Aroclor 1254-induced) in standard Metabolic activation: cofactors TA98 + S9: 5 g/plate benzo[a]pyrene Positive controls: TA98 S9: 0.1g/plate 2-(2-furyl)-3-(5-nitro-2-furyl) – acrylamide TA100 + S9: 5 g/plate benzo[a]pyrene TA100 S9: 0.01g/plate 2-(2-furyl)-3-(5-nitro-2-furyl) – acrylamide TA1535 +S9: 2 g/plate 2-aminoanthracene TA1535 S9: 0.5 g/plate sodium azide – TA1537 + S9: 5 g/plate benzo[a]pyrene TA1537 S9: 80 g/plate 9-aminoacridine – TA1538 + S9: 5 g/plate benzo[a]pyrene TA1538 S9: 2 g/plate 2-nitrofluorene – not stated, similar to OECD TG 471 Test method: no appreciable toxicity was observed at the highest test Comment: concentration of 5000 g/plate; precipitation of the test substance was seen at 500 g/plate and higher, but did not interfere with the validity of the test; all concentrations were tested in triplicate and each test was conducted twice under the conditions of the study, the notified chemical caused no substantial increases in revertant colony numbers over control counts at any concentration in either the presence or absence of the rat liver microsomal enzymes all positive and negative controls responded appropriately the notified chemical was non-mutagenic under the Result: conditions of the assay 9.3.2 HPRT forward mutation assay in V79 Chinese hamster cells (van de in vitro Waart, 1991) V79 Chinese hamster Cells: 10% rat liver S9 fraction (Aroclor 1254-induced) in Metabolic activation system: standard cofactors with S9: 8 mM dimethylnitrosamine in HBSS Positive controls: without S9: 6 mM ethylmethanesulfonate in DMSO
Metabolic activation: 10% rat S9 fraction (Aroclor 1254-induced) in standard cofactors Positive controls: TA98 + S9: 5 g/plate benzo[a]pyrene TA98 – S9: 0.1g/plate 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide TA100 + S9: 5 g/plate benzo[a]pyrene TA100 – S9: 0.01g/plate 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide TA1535 +S9: 2 g/plate 2-aminoanthracene TA1535 – S9: 0.5 g/plate sodium azide TA1537 + S9: 5 g/plate benzo[a]pyrene TA1537 – S9: 80 g/plate 9-aminoacridine TA1538 + S9: 5 g/plate benzo[a]pyrene TA1538 – S9: 2 g/plate 2-nitrofluorene Test method: not stated, similar to OECD TG 471 Comment: no appreciable toxicity was observed at the highest test concentration of 5000 g/plate; precipitation of the test substance was seen at 500 g/plate and higher, but did not interfere with the validity of the test; all concentrations were tested in triplicate and each test was conducted twice under the conditions of the study, the notified chemical caused no substantial increases in revertant colony numbers over control counts at any concentration in either the presence or absence of the rat liver microsomal enzymes all positive and negative controls responded appropriately Result: the notified chemical was non-mutagenic under the conditions of the assay 9.3.2 HPRT forward mutation assay in V79 Chinese hamster cells in vitro (van de Waart, 1991) Cells: V79 Chinese hamster Metabolic activation system: 10% rat liver S9 fraction (Aroclor 1254-induced) in standard cofactors Positive controls: with S9: 8 mM dimethylnitrosamine in HBSS without S9: 6 mM ethylmethanesulfonate in DMSO
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