4-hour (estimated) Alkane 2 Result: was of moderate acute inhalation toxicity to the female rat. 9.1.4 Skin Irritation (Safepharm Laboratories Limited 1995b) Rabbit/New Zealand white Species/strain: 4 females, 2 males Number/sex of animals: 3 days Observation period: A single 4 semi occluded application of 0.5 mL of test Method of administration: substance to intact skin. OECD TG 404; EC Method B4. Test method: Grade 1 erythema (very slight erythema) was observed in 3 Dermal response: female and 1 male rabbit at the 30 minute observation period, persisting in one female and male at the 24 hour observation. No further skin reactions were observed for the remainder of the study. Erythema/Eschar formation: 0.1; 24, 48 & 72 hour group Oedema: 0.0. mean score: Alkane 2 was very slightly irritating to rabbit skin. Result: 9.1.5 Eye Irritation (Safepharm Laboratories Limited 1995o) Rabbit/New Zealand White Species/strain: 6 males, 3 females Number/sex of animals: 1, 24, 48 and 72 hours post instillation Observation period: A single instillation of 0.1 mL of the neat test substance into Method of administration, the conjunctival sac of the right eye of 6 rabbits. The left eye Unirrigated eyes: served as the control.
4-hour (estimated) Result: Alkane 2 was of moderate acute inhalation toxicity to the female rat. 9.1.4 Skin Irritation (Safepharm Laboratories Limited 1995b) Species/strain: Rabbit/New Zealand white Number/sex of animals: 4 females, 2 males Observation period: 3 days Method of administration: A single 4 semi occluded application of 0.5 mL of test substance to intact skin. Test method: OECD TG 404; EC Method B4. Dermal response: Grade 1 erythema (very slight erythema) was observed in 3 female and 1 male rabbit at the 30 minute observation period, persisting in one female and male at the 24 hour observation. No further skin reactions were observed for the remainder of the study. 24, 48 & 72 hour group mean score: Erythema/Eschar formation: 0.1; Oedema: 0.0. Result: Alkane 2 was very slightly irritating to rabbit skin. 9.1.5 Eye Irritation (Safepharm Laboratories Limited 1995o) Species/strain: Rabbit/New Zealand White Number/sex of animals: 6 males, 3 females Observation period: 1, 24, 48 and 72 hours post instillation Method of administration, Unirrigated eyes: A single instillation of 0.1 mL of the neat test substance into the conjunctival sac of the right eye of 6 rabbits. The left eye served as the control.
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