Main study: 0, 50, 150, 500, 1 500 and 5 000 g/plate in all strains. Positive controls: (without S9-mix) N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG) for TA100, TA1535 and WP2uvrA; 9-aminoacridine (9AA) for TA1537; 4-nitroquinoline-1-oxide (4NQO) for TA98. (with S9-mix) 2-aminoanthracene (2AA) for all strains. OECD TG 471 Test method: No cytotoxicity was observed in the study. Comment: Under the conditions of the study, the notified chemical caused no substantial increases in revertant colony numbers over control counts at any concentration in either the presence or absence of the rat liver microsomal enzymes. All positive controls responded appropriately. The notified chemical was non mutagenic under the Result: conditions of the test. 9.3.2 Chromosomal Aberration Assay in Chinese Hamster Lung Cells (Safepharm Laboratories Ltd, 1998f) Chinese Hamster Lung (CHL) Cells Liver fraction (S9 mix) from rats pretreated with Aroclor Metabolic activation 1254. system: Duplicates were performed at each concentration. Dosing schedule: Metabolic Experiment Test concentration (g/mL) Controls Activation Number -S9 1 12 hr continuous treatment 0, 78.1, 156.25, 312.5*, 625*, 1 250* and 1875
Main study: 0, 50, 150, 500, 1 500 and 5 000 g/plate in all strains. Positive controls: (without S9-mix) N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG) for TA100, TA1535 and WP2uvrA; 9-aminoacridine (9AA) for TA1537; 4-nitroquinoline-1-oxide (4NQO) for TA98. (with S9-mix) 2-aminoanthracene (2AA) for all strains. Test method: OECD TG 471 Comment: No cytotoxicity was observed in the study. Under the conditions of the study, the notified chemical caused no substantial increases in revertant colony numbers over control counts at any concentration in either the presence or absence of the rat liver microsomal enzymes. All positive controls responded appropriately. Result: The notified chemical was non mutagenic under the conditions of the test. 9.3.2 Chromosomal Aberration Assay in Chinese Hamster Lung Cells (Safepharm Laboratories Ltd, 1998f) Chinese Hamster Lung (CHL) Cells Metabolic activation system: Liver fraction (S9 mix) from rats pretreated with Aroclor 1254. Dosing schedule: Duplicates were performed at each concentration. Metabolic Activation Experiment Number Test concentration (g/mL) Controls -S9 1 12 hr continuous treatment 0, 78.1, 156.25, 312.5*, 625*, 1 250* and 1875
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