Attachment 1 The Draize Scale (Draize, 1959) for evaluation of skin reactions is as follows: Erythema Formation Rating Oedema Formation Rating No erythema 0 No oedema 0 Very slight erythema (barely perceptible) 1 Very slight oedema (barely perceptible) 1 Well-defined erythema 2 Slight oedema (edges of area well- 2 defined by definite raising Moderate to severe erythema 3 Moderate oedema (raised approx. 1 mm) 3 Severe erythema (beet redness) 4 Severe oedema (raised more than 1 mm 4 and extending beyond area of exposure) The Draize scale (Draize 1944) for evaluation of eye reactions is as follows: et al., CORNEA Opacity Rating Area of Cornea involved Rating No opacity 0 none 25% or less (not zero) 1 Diffuse area, details of iris clearly visible 1 slight 25% to 50% 2 Easily visible translucent areas, details of iris slightly obscure 2 mild 50% to 75% 3 Opalescent areas, no details of iris 3 visible, size of pupil barely discernible Greater than 75% 4 moderate Opaque, iris invisible 4 severe area around eye Swelling with lids half- closed individual vessels not easily discernible Swelling with lids half- adjacent 3 severe mod. closed to completely Diffuse beefy red Discharge with closed moistening of lids and severe hairs and considerable 3 severe IRIS Values Rating Normal 0 none Folds above normal, congestion, swelling, circumcorneal injection, iris reacts to light 1 slight No reaction to haemorrhage, gross destruction 2 severe Draize, J. H., Woodward, G., Calvery, H. O. (1944) Methods for the of Irritation and Toxicity of Substances Applied Topically to the Skin and Mucous Membranes, J. Pharmacol. Exp. Ther. 82 : 377- 390. Draize J. H. (1959) Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics. Association of Food and Drug Officials of the US, 49 : 2-56.
Attachment 1 The Draize Scale (Draize, 1959) for evaluation of skin reactions is as follows: Erythema Formation Rating Oedema Formation Rating No erythema 0 No oedema 0 Very slight erythema (barely perceptible) 1 Very slight oedema (barely perceptible) 1 Well-defined erythema 2 Slight oedema (edges of area well- 2 defined by definite raising Moderate to severe erythema 3 Moderate oedema (raised approx. 1 mm) 3 Severe erythema (beet redness) 4 Severe oedema (raised more than 1 mm 4 and extending beyond area of exposure) The Draize scale (Draize et al., 1944) for evaluation of eye reactions is as follows: CORNEA Opacity Rating Area of Cornea involved Rating No opacity 0 none 25% or less (not zero) 1 Diffuse area, details of iris clearly visible Easily visible translucent areas, details of iris slightly obscure 1 slight 25% to 50% 2 2 mild 50% to 75% 3 Opalescent areas, no details of iris visible, size of pupil barely discernible 3 moderate Greater than 75% 4 Opaque, iris invisible 4 severe individual vessels not easily discernible Diffuse beefy red 3 severe Swelling with lids half- closed Swelling with lids half- closed to completely closed mod. severe adjacent Discharge with moistening of lids and hairs and considerable area around eye 3 severe IRIS Values Rating Normal 0 none Folds above normal, congestion, swelling, circumcorneal injection, iris reacts to light 1 slight No reaction to haemorrhage, gross destruction 2 severe Draize, J. H., Woodward, G., Calvery, H. O. (1944) Methods for the of Irritation and Toxicity of Substances Applied Topically to the Skin and Mucous Membranes, J. Pharmacol. Exp. Ther. 82 : 377- 390. Draize J. H. (1959) Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics. Association of Food and Drug Officials of the US, 49 : 2-56.
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