WP2uvrA Strains: E. coli Microsomal fraction (S9) from Aroclor 1254-induced rat Metabolic activation: liver homogenate. 0, 10, 50, 100, 500, 1000 or 5000 g/plate (maximum of 2500 Concentration range: g/plate in the duplicate assay). OECD TG 471 Test method: Positive controls used were 2-anthramine to test the efficacy Comment: of the metabolic activation system and Positive controls demonstrated the sensitivity of the test system and negative controls were within historical limits. The test substance was non mutagenic under the conditions Result: of the test and no increase in the mean number of revertants above background occurred. 9.3.1.3 TM-599 (SRI International, 1993b) TA 1535, TA 1537, TA 98, TA 100 Strains: S. typhimurium Microsomal fraction (S9) from Aroclor 1254-induced rat Metabolic activation: homogenate. 0, 10, 50, 100, 500, 1000 or 5000 g/plate (maximum of 2500 Concentration range: g/plate in the duplicate assay). OECD TG 471 Test method: Positive controls used were 2-anthramine to test the efficacy Comment: of the metabolic activation system, sodium and 2-nitrofluorene. Positive controls demonstrated the sensitivity of the test system and negative controls were within historical limits. The test substance was non mutagenic under the conditions Result: of the test and no increase in the mean number of revertants above background occurred. 9.3.1.4 TM-599D (SRI International, 1993c) WP2uvrA Strains: E. coli
Strains: E. coli WP2uvrA Metabolic activation: Microsomal fraction (S9) from Aroclor 1254-induced rat liver homogenate. Concentration range: 0, 10, 50, 100, 500, 1000 or 5000 g/plate (maximum of 2500 g/plate in the duplicate assay). Test method: OECD TG 471 Comment: Positive controls used were 2-anthramine to test the efficacy of the metabolic activation system and Positive controls demonstrated the sensitivity of the test system and negative controls were within historical limits. Result: The test substance was non mutagenic under the conditions of the test and no increase in the mean number of revertants above background occurred. 9.3.1.3 TM-599 (SRI International, 1993b) Strains: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 Metabolic activation: Microsomal fraction (S9) from Aroclor 1254-induced rat homogenate. Concentration range: 0, 10, 50, 100, 500, 1000 or 5000 g/plate (maximum of 2500 g/plate in the duplicate assay). Test method: OECD TG 471 Comment: Positive controls used were 2-anthramine to test the efficacy of the metabolic activation system, sodium and 2-nitrofluorene. Positive controls demonstrated the sensitivity of the test system and negative controls were within historical limits. Result: The test substance was non mutagenic under the conditions of the test and no increase in the mean number of revertants above background occurred. 9.3.1.4 TM-599D (SRI International, 1993c) Strains: E. coli WP2uvrA
NA883FR.docx