1 male, 2 female Number/sex of animals: 3 days Observation period: 0.5 g of test material, moistened with bi-distilled water, was Method of administration: applied to a clipped intact region of the dorsal skin and secured under a gauze patch with a semi-occlusive dressing for 4 hours; at the end of this time residual material was removed with lukewarm water; animals were examined for skin reaction 1, 24, 48 and 72 hours following application of the test substance OECD TG 404 Test method: Draize scores were all zero at all time intervals; light orange Observations: staining of the treated skin persisted throughout the study the notified chemical was not irritating to the skin of rabbits Result: 9.1.5 Eye Irritation (Braun, 1997a) rabbit/New Zealand White Species/strain: 1 male, 2 female Number/sex of animals: 14 days Observation period: 0.1 g of test material applied as supplied into conjunctival Method of administration: sac of the left eye of each animal; the contralateral eye served as the control; animals were examined for eye lesions 1, 24, 48 and 72 after test substance application; further observations were made after 7 and 14 days OECD TG 405 Test method: chemosis was observed in one animal and discharge was Observations observed in all of the animals 1 hour after application of the test material; all Draize scores were zero for the remainder of the study; light orange staining of the conjunctivae and sclera of the treated eyes was observed in all animals at 24 and 48 hours; staining was restricted to the nictating membrane and lid hairs after this time; staining of the nictating membrane had cleared by day 14 the notified chemical was not irritating to the eyes of rabbits Result: 9.1.6 Skin Sensitisation (Maximisation Test) (Arcelin, 1997b)
Number/sex of animals: 1 male, 2 female Observation period: 3 days Method of administration: 0.5 g of test material, moistened with bi-distilled water, was applied to a clipped intact region of the dorsal skin and secured under a gauze patch with a semi-occlusive dressing for 4 hours; at the end of this time residual material was removed with lukewarm water; animals were examined for skin reaction 1, 24, 48 and 72 hours following application of the test substance Test method: OECD TG 404 Observations: Draize scores were all zero at all time intervals; light orange staining of the treated skin persisted throughout the study Result: the notified chemical was not irritating to the skin of rabbits 9.1.5 Eye Irritation (Braun, 1997a) Species/strain: rabbit/New Zealand White Number/sex of animals: 1 male, 2 female Observation period: 14 days Method of administration: 0.1 g of test material applied as supplied into conjunctival sac of the left eye of each animal; the contralateral eye served as the control; animals were examined for eye lesions 1, 24, 48 and 72 after test substance application; further observations were made after 7 and 14 days Test method: OECD TG 405 Observations chemosis was observed in one animal and discharge was observed in all of the animals 1 hour after application of the test material; all Draize scores were zero for the remainder of the study; light orange staining of the conjunctivae and sclera of the treated eyes was observed in all animals at 24 and 48 hours; staining was restricted to the nictating membrane and lid hairs after this time; staining of the nictating membrane had cleared by day 14 Result: the notified chemical was not irritating to the eyes of rabbits 9.1.6 Skin Sensitisation (Maximisation Test) (Arcelin, 1997b)
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