9.2.3 Up to 51 days, oral Rat/ Sprague Dawley 1-tetradecene (blend from three suppliers) NOAEL = 1 000 mg/kg/day Crl:CD BR VAF/Plus for reproductive neurotoxic effects; NOAEL = 100 mg/kg/day for systemic effects in females. No for males 9.2.4 13-week, oral Rat/ Crl:CD BR C20 - C24 Alkenes, Branched and Linear NOAEL = 500 mg/kg/day 9.3 Genetic Toxicity 9.3.1 in vitro 9.3.1.1 Bacterial reverse mutation S.typhimurium, E.coli C20 - C24 Alkenes, branched and linear Negative with & w’out activation 9.3.1.2 Unscheduled DNA Rat hepatocyte primary Gulftene 12 16 Negative – Synthesis culture 9.3.1.3 BALB/3T3 Transformation Mouse embryo cells Gulftene 12 16 Negative – Test BALB/3T3-A31-1-1 9.3.1.4 Assay Chinese Hamster Gulftene 12 16 Negative with & w’out – cell activation 9.3.1.5 Chromosome aberration Human peripheral C20-C24 Alkenes, branched and linear Negative with & w’out lymphocytes activation 9.3.1.6 Other Alkenes Bacterial Reverse Mutation 1-Octadecene Negative with & w’out S.typhimurium, E.coli Assay activation Mitotic Recombination Rat liver RL1 cells. 1-Octadecene Negative with & w’out activation Chromosome Rat liver RL1 cells. 1-Octadecene Negative with & w’out AberrationH18 activation 9.3.2 in vivo
9.2.3 Up to 51 days, oral Rat/ Sprague Dawley Crl:CD BR VAF/Plus 1-tetradecene (blend from three suppliers) NOAEL = 1 000 mg/kg/day for reproductive neurotoxic effects; NOAEL = 100 mg/kg/day for systemic effects in females. No for males 9.2.4 13-week, oral Rat/ Crl:CD BR C20 - C24 Alkenes, Branched and Linear NOAEL = 500 mg/kg/day 9.3 Genetic Toxicity 9.3.1 in vitro 9.3.1.1 Bacterial reverse mutation S.typhimurium, E.coli C20 - C24 Alkenes, branched and linear Negative with & w’out activation 9.3.1.2 Unscheduled DNA Synthesis Rat hepatocyte primary culture Gulftene 12 – 16 Negative 9.3.1.3 BALB/3T3 Transformation Test Mouse embryo cells BALB/3T3-A31-1-1 Gulftene 12 – 16 Negative 9.3.1.4 Assay Chinese Hamster cell Gulftene 12 – 16 Negative with & w’out activation 9.3.1.5 Chromosome aberration Human peripheral lymphocytes C20-C24 Alkenes, branched and linear Negative with & w’out activation 9.3.1.6 Other Alkenes Bacterial Reverse Mutation Assay S.typhimurium, E.coli 1-Octadecene Negative with & w’out activation Mitotic Recombination Rat liver RL1 cells. 1-Octadecene Negative with & w’out activation Chromosome AberrationH18 Rat liver RL1 cells. 1-Octadecene Negative with & w’out activation 9.3.2 in vivo
NA844FR.docx