increases, or dose relationships were observed at any other dose or exposure time. Under the conditions of this study Alkane 4 is not considered clastogenic in vivo. Hazard Classification Under the NOHSC (NOHSC 1999) Approved Criteria for Classifying Hazardous Substances, Alkane 4 would not be classified as a hazardous substance. 10. ASSESSMENT OF ENVIRONMENTAL EFFECTS Tests were performed according to EC and OECD test guidelines (European Commission 1992), (OECD 1995-1996) at Safepharm Laboratories Limited UK. These facilities comply with the OECD principles of GLP and full test reports were submitted. All tests were performed on Alkane 4. 10.1 Summary of Effects on Biotic Systems Test Species Results Acute Toxicity Rainbow trout LLR50 b > 1 000 mg/L Semi-static WAF a (Oncorhynchus NOEC 1 000 mg/L mykiss) ≥ 96 hour OECD TG 203 Acute Immobilisation Water Flea ELR50 c > 1 000 mg/L Static WAF a 48 hour (Daphnia NOEC 1 000 mg/L magna) ≥ OECD TG 202 Growth Inhibition Algae EbLR50 d > 1 000 mg/L Static 96 hour (Selenastrum EµLR50 e > 1 000 mg/L capricornutum) OECD TG 201 NOEC 1 000 mg/L ≥ WAF: water accommodated fraction - see comments in text below. LLR: lethal loading rate. ELR: effective loading rate. EbLR50: effective loading rate that reduced biomass by 50%. EµLR 50: effective loading rate that reduced specific growth rate by 50% (24-48 hours). f) NOEC - no observable effect concentration. Based on the results of rangefinding studies, limit tests were conducted for the definitive studies. The toxicity of Alkane 4 on fish, water flea and algae was examined using a Water Accommodated Fraction (WAF) with a loading rate of 1 000 mg/L. The test media was stirred for 20 hours, with the mixture then allowed to stand for 4 hours prior to the removal of the aqueous phase or WAF.
increases, or dose relationships were observed at any other dose or exposure time. Under the conditions of this study Alkane 4 is not considered clastogenic in vivo. Hazard Classification Under the NOHSC Approved Criteria for Classifying Hazardous Substances, (NOHSC 1999) Alkane 4 would not be classified as a hazardous substance. 10. ASSESSMENT OF ENVIRONMENTAL EFFECTS Tests were performed according to EC and OECD test guidelines (European Commission 1992), (OECD 1995-1996) at Safepharm Laboratories Limited UK. These facilities comply with the OECD principles of GLP and full test reports were submitted. All tests were performed on Alkane 4. 10.1 Summary of Effects on Biotic Systems Test Species Results Acute Toxicity Semi-static WAF a 96 hour OECD TG 203 Rainbow trout (Oncorhynchus mykiss) LLR50 b > 1 000 mg/L NOEC ≥ 1 000 mg/L Acute Immobilisation Static WAF a 48 hour OECD TG 202 Water Flea (Daphnia magna) ELR50 c > 1 000 mg/L NOEC ≥ 1 000 mg/L Growth Inhibition Static 96 hour OECD TG 201 Algae (Selenastrum capricornutum) EbLR50 d > 1 000 mg/L EµLR50 e > 1 000 mg/L NOEC ≥ 1 000 mg/L WAF: water accommodated fraction - see comments in text below. LLR: lethal loading rate. ELR: effective loading rate. EbLR50: effective loading rate that reduced biomass by 50%. EµLR 50: effective loading rate that reduced specific growth rate by 50% (24-48 hours). f) NOEC - no observable effect concentration. Based on the results of rangefinding studies, limit tests were conducted for the definitive studies. The toxicity of Alkane 4 on fish, water flea and algae was examined using a Water Accommodated Fraction (WAF) with a loading rate of 1 000 mg/L. The test media was stirred for 20 hours, with the mixture then allowed to stand for 4 hours prior to the removal of the aqueous phase or WAF.
NA849FR.docx