-S9 0, 2, 5, 10, 20, 0, 156, 313 0, 39, 78 0, 5, 10, 20, 39, 78 625, 1250 156, 313 39, 78, 156 2500, 5000 625, 1250 +S9 0, 20, 39, 78, 0, 313, 625, 0, 20, 39, 78, 156, 313, 156, 313, 625 1250, 2500 625 5000 OECD TG 471 Test method: Solvent control: dimethylsulfoxide; Postive controls: (a) S9: Comment: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2): TA 98, TA 100 and WP2uvrA; sodium azide, TA 1535; TA 1537; (b) + S9: benzo(a)pyrene, TA 100, TA 98 and TA 1537; 2-aminoanthracene, TA 1535, WP2uvrA. Similar numbers of back mutant colonies were observed regardless of the addition of the notified chemical to bacterial suspensions to plating with or without rat liver S9 fraction was included. Negative controls were within historical limits and positive controls demonstrated the sensitivity of the test system. The notified chemical was non mutagenic under the Result: conditions of the test. 9.3.2 Chromosomal Aberration Assay in Chinese Hamster Lung (CHL) Cells (Shin Nippon Biomedical Laboratories Ltd, 2001) CHL Cells: S9 fraction from the livers of male Sprague-Dawley rats Metabolic activation treated with phenobarbital/ 5,6-benzoflavone. system: Dosing schedule: Metabolic Experiment Test concentration (microgram/mL) Controls Activation Number -S9 1 treatment time = 6 hours, harvest time = 24 hours
-S9 0, 2, 5, 10, 20, 39, 78 0, 156, 313 625, 1250 2500, 5000 0, 39, 78 156, 313 625, 1250 0, 5, 10, 20, 39, 78, 156 +S9 0, 20, 39, 78, 156, 313, 625 0, 313, 625, 1250, 2500 5000 0, 20, 39, 78, 156, 313, 625 Test method: OECD TG 471 Comment: Solvent control: dimethylsulfoxide; Postive controls: (a) S9: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2): TA 98, TA 100 and WP2uvrA; sodium azide, TA 1535; TA 1537; (b) + S9: benzo(a)pyrene, TA 100, TA 98 and TA 1537; 2-aminoanthracene, TA 1535, WP2uvrA. Similar numbers of back mutant colonies were observed regardless of the addition of the notified chemical to bacterial suspensions to plating with or without rat liver S9 fraction was included. Negative controls were within historical limits and positive controls demonstrated the sensitivity of the test system. Result: The notified chemical was non mutagenic under the conditions of the test. 9.3.2 Chromosomal Aberration Assay in Chinese Hamster Lung (CHL) Cells (Shin Nippon Biomedical Laboratories Ltd, 2001) Cells: CHL Metabolic activation system: S9 fraction from the livers of male Sprague-Dawley rats treated with phenobarbital/ 5,6-benzoflavone. Dosing schedule: Metabolic Activation Experiment Number Test concentration (microgram/mL) Controls -S9 1 treatment time = 6 hours, harvest time = 24 hours
NA946FR.docx