Number of animals exhibiting positive responses at challenge: Test animals Control animals Challenge 24 48 72 24 hours 48 hours 72 concentration hours* 30% **3/10 3/10 3/10 0/5 0/5 0/5 10% 0/10 0/10 0/10 0/5 0/5 0/5 5% 0/10 0/10 0/10 0/5 0/5 0/5 0% 0/10 0/10 0/10 0/5 0/5 0/5 * time after patch removal ** number of animals exhibiting positive responses Challenge outcome: Challenge concentration of 30%: 2 animals had well defined erythema and one animal had very slight erythema, which persisted to the 72 hour observation time. Challenge concentrations of 0, 5, or 10% revealed no discernable reactions. Control group - no discernable reactions. Result: Magnol R was sensitising to guinea pig skin. Skin Sensitisation Buehler Method on Magnol (Magnol-R and Triethyl Citrate 50:50 w/w%) (Takahashai, 1996) guinea pig/Hartley Species/strain: 10 females (test group), 5 females (control group) Number of animals: Magnol (Magnol-R and Triethyl Citrate 50:50 w/w%) Test substance: Similar to OECD TG 406 Test method: test animals: Induction procedure: Days 1, 7 and 14: lint cloth soaked with 0.5 mL of 5% Magnol in vaseline was applied to the shorn left ventral skin and held under occlusive dressing for 6 hours; control animals: treated similarly to the test animals omitting the test substance from the topical application
Number of animals exhibiting positive responses at challenge: Challenge Test animals Control animals concentration 24 48 72 hours* 24 hours 48 hours 72 30% **3/10 3/10 3/10 0/5 0/5 0/5 10% 0/10 0/10 0/10 0/5 0/5 0/5 5% 0/10 0/10 0/10 0/5 0/5 0/5 0% 0/10 0/10 0/10 0/5 0/5 0/5 * time after patch removal ** number of animals exhibiting positive responses Challenge outcome: Challenge concentration of 30%: 2 animals had well defined erythema and one animal had very slight erythema, which persisted to the 72 hour observation time. Challenge concentrations of 0, 5, or 10% revealed no discernable reactions. Control group - no discernable reactions. Result: Magnol R was sensitising to guinea pig skin. Skin Sensitisation Buehler Method on Magnol (Magnol-R and Triethyl Citrate 50:50 w/w%) (Takahashai, 1996) Species/strain: guinea pig/Hartley Number of animals: 10 females (test group), 5 females (control group) Test substance: Magnol (Magnol-R and Triethyl Citrate 50:50 w/w%) Test method: Similar to OECD TG 406 Induction procedure: test animals: Days 1, 7 and 14: lint cloth soaked with 0.5 mL of 5% Magnol in vaseline was applied to the shorn left ventral skin and held under occlusive dressing for 6 hours; control animals: treated similarly to the test animals omitting the test substance from the topical application
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