the knockdown efficiencies of and Nupr1, Sox2, TEADs, Last1/2, p53, Nestin, Tert siRNAs. Cells were transfected with negative control siRNA, siRNA#1, #2, Nupr1 shRNA#1, #2, siRNA#1, #2, siRNA#1, #2, p53 siRNA#1, #2, Sox2 TEADs Last1/2 siRNA or siRNA for 24 hrs. Real time PCRs were performed to (i) Nestin Tert quantify the overexpression efficiency. Cells were transfected with Empty vector or Nupr1 cDNA plasmid. Total mRNAs were extracted for quantitative analysis by real time PCR. Mean ± s.e.m.; n=3 independent experiments for all subfigures; *p<0.05; **p<0.01; *** Note that in (c), silencing had no effects on p<0.001. Sox2 p53, Nestin, or expression; ns=not statistically significant. Tert Supplementary Table 1. Sequences of primers for real time qPCR. Supplementary Table 2. Sequences of siRNAs or shRNAs.
the knockdown efficiencies of Nupr1, Sox2, TEADs, Last1/2, p53, Nestin, and Tert siRNAs. Cells were transfected with negative control siRNA, Nupr1 siRNA#1, #2, Sox2 shRNA#1, #2, TEADs siRNA#1, #2, Last1/2 siRNA#1, #2, p53 siRNA#1, #2, Nestin siRNA or Tert siRNA for 24 hrs. (i) Real time PCRs were performed to quantify the overexpression efficiency. Cells were transfected with Empty vector or Nupr1 cDNA plasmid. Total mRNAs were extracted for quantitative analysis by real time PCR. Mean ± s.e.m.; n=3 independent experiments for all subfigures; *p<0.05; **p<0.01; *** p<0.001. Note that in (c), silencing Sox2 had no effects on p53, Nestin, or Tert expression; ns=not statistically significant. Supplementary Table 1. Sequences of primers for real time qPCR. Supplementary Table 2. Sequences of siRNAs or shRNAs.
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