the cuticle granules are pre-formed by condensation of mussel foot protein-1 (mcfp-1) through extensive Dopa-Fe3+-complexation in the accessory gland (Harrington et al., 2010). The cuticle matrix consists of both mfp-1 and an acidic, chymotrypsin-labile, Ca2+-binding protein (Holten-Andersen et al., 2009a; Vitellaro Zuccarello, 1981). The flexible junctions between the granules and the matrix absorb and dissipate the impact of the high-energy surf by micro-cracking (Holten-Andersen et al., 2007) and reversible deformation (Bertoldi and Boyce, 2007; Carrington, E, Gosline JM, 2006), thereby minimizing damage to the byssus and preventing catastrophic failure. Based on these observations, understanding the molecular composition and hierarchical structure of the cuticle may help engineer hard, strain-tolerant materials. Chapter 2. Underwater delivery: Coacervation of mcfp-1 and hyaluronic acid Portions of this chapter have been printed as: Dusty Rose Miller, Saurabh Das, Huang, Songi Han, Jacob N. Israelachvili and J. Herbert Waite, adhesive “Mussel protein-derived complex coacervates mitigate frictional surface damage.” Submitted to Bioscience and Bioengineering June, 2015.
the cuticle granules are pre-formed by condensation of mussel foot protein-1 (mcfp-1) through extensive Dopa-Fe3+-complexation in the accessory gland (Harrington et al., 2010). The cuticle matrix consists of both mfp-1 and an acidic, chymotrypsin-labile, Ca2+-binding protein (Holten-Andersen et al., 2009a; Vitellaro Zuccarello, 1981). The flexible junctions between the granules and the matrix absorb and dissipate the impact of the high-energy surf by micro-cracking (Holten-Andersen et al., 2007) and reversible deformation (Bertoldi and Boyce, 2007; Carrington, E, Gosline JM, 2006), thereby minimizing damage to the byssus and preventing catastrophic failure. Based on these observations, understanding the molecular composition and hierarchical structure of the cuticle may help engineer hard, strain-tolerant materials. Chapter 2. Underwater delivery: Coacervation of mcfp-1 and hyaluronic acid Portions of this chapter have been printed as: Dusty Rose Miller, Saurabh Das, Huang, Songi Han, Jacob N. Israelachvili and J. Herbert Waite, “Mussel adhesive protein-derived complex coacervates mitigate frictional surface damage.” Submitted to Bioscience and Bioengineering June, 2015.
Miller_dissertation_corrections.docx