CHL Cells: Phenobarbitone/ -naphthoflavone-induced Sprague-Dawley Metabolic activation rat liver S9 fraction. system: Dosing schedule: Experiment Test concentration (g/mL) Controls Activation Number -S9 1 treatment time = 6 hours, harvest time Positive: mitomycin = 24 hours C, 0.1 g/mL 0*, 1, 2*, 4*, 8*, 12*, 16 g/mL Negative: acetone vehicle 2 treatment time = 24 hours = harvest time Positive: mitomycin C, 0.05 g/mL 0*, 2, 4*, 8*, 10*, 12, 16 g/mL treatment time = 48 hours = harvest time Positive: mitomycin C, 0.025 g/mL 0*, 1, 2, 4*, 8*, 10*, 12 g/mL Negative: acetone vehicle +S9 1 treatment time = 6 hours, harvest time Positive: = 24 hours cyclophosphamide, 0*, 105, 210, 420, 840*, 1680*, 2100* 10 g/mL g/mL Negative: acetone vehicle 2 treatment time = 6 hours, harvest time = 24 hours Positive: cyclophosphamide, 10 g/mL 0*, 450, 900, 1800, 2100*, 2400*, Negative: acetone 2700* g/mL vehicle * selected for metaphase analysis OECD TG 473 Test method: The test substance was shown to be toxic and doses were Comment: selected accordingly. Statistically significant increases in
Cells: CHL Metabolic activation system: Phenobarbitone/ -naphthoflavone-induced Sprague-Dawley rat liver S9 fraction. Dosing schedule: Activation Experiment Number Test concentration (g/mL) Controls -S9 1 treatment time = 6 hours, harvest time = 24 hours Positive: mitomycin C, 0.1 g/mL Negative: acetone vehicle Positive: mitomycin C, 0.05 g/mL Positive: mitomycin C, 0.025 g/mL Negative: acetone vehicle 0*, 1, 2*, 4*, 8*, 12*, 16 g/mL 2 treatment time = 24 hours = harvest time 0*, 2, 4*, 8*, 10*, 12, 16 g/mL treatment time = 48 hours = harvest time 0*, 1, 2, 4*, 8*, 10*, 12 g/mL +S9 1 treatment time = 6 hours, harvest time = 24 hours Positive: cyclophosphamide, 10 g/mL Negative: acetone vehicle Positive: cyclophosphamide, 10 g/mL 0*, 105, 210, 420, 840*, 1680*, 2100* g/mL 2 treatment time = 6 hours, harvest time = 24 hours 0*, 450, 900, 1800, 2100*, 2400*, 2700* g/mL Negative: acetone vehicle * selected for metaphase analysis Test method: OECD TG 473 Comment: The test substance was shown to be toxic and doses were selected accordingly. Statistically significant increases in
NA907FR.docx