Place the loop outside of the starting midline to the left and move the loop across the midline to the right. Continue streaking for about 1/3 of the plate. See image above. Rotate the plate a 1/3 turn. Flame and sterilize your loop. Place the loop outside the streaked area. Move through the streaked area one time then continue streaking away from the first area. Streak for 1/3 of the plate, being careful not to cross the loop through the original streak. Rotate another 1/3 of a turn, flame your loop, and repeat. This will create individual colonies since you are essentially spreading out the bacteria and isolating one bacterium from another. Replace the lid, re-flame the loop and let it cool. Tape the lid shut. You will be incubating the inoculated plates, lid-side down, for 24-48 hours. Note the incubation temperature of 37 degrees Celsius in your lab manual. Lab 3: Observation of Cultures & Colony Morphology Purpose: To practice observing broth and agar cultures for evidence of growth, as well as to learn to classify colonies by specific descriptors. Background: Bacteria have exceedingly high of binary fission and are therefore easy to culture in a lab. Evidence of growth in a broth medium will be seen as a cloudy appearance in a previously
Place the loop outside of the starting midline to the left and move the loop across the midline to the right. Continue streaking for about 1/3 of the plate. See image above. Rotate the plate a 1/3 turn. Flame and sterilize your loop. Place the loop outside the streaked area. Move through the streaked area one time then continue streaking away from the first area. Streak for 1/3 of the plate, being careful not to cross the loop through the original streak. Rotate another 1/3 of a turn, flame your loop, and repeat. This will create individual colonies since you are essentially spreading out the bacteria and isolating one bacterium from another. Replace the lid, re-flame the loop and let it cool. Tape the lid shut. You will be incubating the inoculated plates, lid-side down, for 24-48 hours. Note the incubation temperature of 37 degrees Celsius in your lab manual. Lab 3: Observation of Cultures & Colony Morphology Purpose: To practice observing broth and agar cultures for evidence of growth, as well as to learn to classify colonies by specific descriptors. Background: Bacteria have exceedingly high of binary fission and are therefore easy to culture in a lab. Evidence of growth in a broth medium will be seen as a cloudy appearance in a previously
Microbiology%20Lab%20Manual_Newbury%20Park.docx