9.1.4 Skin Irritation (Pharma Research Toxicology and Pathology, 1987d) Analogue A Test Substance: Rabbit/New Zealand Albino Species/strain: 3 Number of Animals: 7 days Observation period: A semi-occlusive application of 0.5 mL undiluted test Method of Administration: substance was made on the dorsal skin. After 4 hours, the substance carefully removed with tap water. TG 404 Test Method: Draize scores: Time after Animal # treatment 1 2 3 (days) Erythema 1 a2 2 3 2 2 2 2 3 0 2 2 Oedema 1 1 0 1 2 1 0 1 3 0 0 0 a see Attachment 1 for Draize scales Erythema and scab formation: 1.3, 2.0, 2.3. Mean Individual Scores Oedema: 0.7, 0.0, 0.7 (24,48 & 72 hour observations: By the seventh day post-treatment, all signs of irritation had Comment: disappeared except for one animal, which still had dry and brittle skin. Analogue A was moderately irritating to the skin of rabbits. Result:
9.1.4 Skin Irritation (Pharma Research Toxicology and Pathology, 1987d) Test Substance: Analogue A Species/strain: Rabbit/New Zealand Albino Number of Animals: 3 Observation period: 7 days Method of Administration: A semi-occlusive application of 0.5 mL undiluted test substance was made on the dorsal skin. After 4 hours, the substance carefully removed with tap water. Test Method: TG 404 Draize scores: Time after Animal # treatment (days) 1 2 3 Erythema 1 a2 2 3 2 2 2 2 3 0 2 2 Oedema 1 1 0 1 2 1 0 1 3 0 0 0 a see Attachment 1 for Draize scales Mean Individual Scores (24,48 & 72 hour observations: Erythema and scab formation: 1.3, 2.0, 2.3. Oedema: 0.7, 0.0, 0.7 Comment: By the seventh day post-treatment, all signs of irritation had disappeared except for one animal, which still had dry and brittle skin. Result: Analogue A was moderately irritating to the skin of rabbits.
NA837FR.docx