Genotoxicity Reverse Mutation Assay (23% Notified Chemical) Salmonella typhimurium (Shapiro, 1989a) TA98 and TA1535 Strains: S. typhimurium 0.05 1.0 with and without S-9 Concentration range: g/plate – similar to OECD TG 471 (Organisation for Test method: Economic Co-operation and Development, 1995- 1996) the test material (23% notified chemical) did Result: not induce mutation in strains S. typhimurium TA98 and TA1535 in the absence or presence of metabolic activation Reverse Mutation Assay (23% Notified Chemical) Salmonella typhimurium (Ballantyne, 1996) TA98, TA100, TA1535 and Strains: S. typhimurium TA1537 experiment 1: 0.16, 0.8, 4, 20 and 100 g/plate Concentration without S-9, and 0.8, 4, 20, 100 and 500 g/plate with S-9; experiment 2: 3.125, 6.25, 12.5, 25 and 50 g/plate without S-9, and 12.5, 25, 50, 100 and 200 with S-9 g/plate similar to OECD TG 471 (Organisation for Test method: Economic Co-operation and Development, 1995- 1996) the test material (23% notified chemical) did not Result: induce mutation in four strains of S. typhimurium in the absence or presence of metabolic activation
Genotoxicity Salmonella typhimurium Reverse Mutation Assay (23% Notified Chemical) (Shapiro, 1989a) Strains: S. typhimurium TA98 and TA1535 Concentration range: 0.05 – 1.0 g/plate with and without S-9 Test method: similar to OECD TG 471 (Organisation for Economic Co-operation and Development, 1995- 1996) Result: the test material (23% notified chemical) did not induce mutation in S. typhimurium strains TA98 and TA1535 in the absence or presence of metabolic activation Salmonella typhimurium Reverse Mutation Assay (23% Notified Chemical) (Ballantyne, 1996) Strains: S. typhimurium TA98, TA100, TA1535 and TA1537 Concentration experiment 1: 0.16, 0.8, 4, 20 and 100 g/plate without S-9, and 0.8, 4, 20, 100 and 500 g/plate with S-9; experiment 2: 3.125, 6.25, 12.5, 25 and 50 g/plate without S-9, and 12.5, 25, 50, 100 and 200 g/plate with S-9 Test method: similar to OECD TG 471 (Organisation for Economic Co-operation and Development, 1995- 1996) Result: the test material (23% notified chemical) did not induce mutation in four strains of S. typhimurium in the absence or presence of metabolic activation
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