0, 25, 150 or 1 000 mg/kg/day for 28 days Dose/Study duration:: no clinical signs and no bodyweight changes or Clinical observations: changes in food consumption in any dose group Clinical chemistry/Haematology no significant observations no macroscopic findings and no organ weight Macroscopic findings: changes the livers of all males and some females at the top Histopathology: dose exhibited slight centrilobular hypertrophy not specified Test method: the NOEL was 150 mg/kg/day on the basis of Result: liver centrilobular hypertrophy at 1 000 mg/kg/day Genotoxicity Reverse Mutation Assay with Shell Brake Fluid Dot 4 Salmonella typhimurium (Brooks & Wiggins, 1992) Shell Brake Fluid Dot 4 strains TA 1535, Strains: Salmonella typhimurium TA 1537, TA 1538, TA 98, TA 100 and WP2 Escherichia coli uvrA(pKM101) 31.1 5 000 g/plate Concentration range: – Maron and Ames (Maron & Ames, 1983): Test method: metabolic activation was provided by Aroclor 1254-induced rat liver S9 fraction; positive control compounds to check that the strains exhibited the appropriate responses were benzo(a)pyrene, potassium dichromate, neutral red, sodium azide, 2- nitrofluorene, 2-aminoanthracene and 9- aminoacridine
Dose/Study duration:: 0, 25, 150 or 1 000 mg/kg/day for 28 days Clinical observations: no clinical signs and no bodyweight changes or changes in food consumption in any dose group Clinical chemistry/Haematology no significant observations Macroscopic findings: no macroscopic findings and no organ weight changes Histopathology: the livers of all males and some females at the top dose exhibited slight centrilobular hypertrophy Test method: not specified Result: the NOEL was 150 mg/kg/day on the basis of liver centrilobular hypertrophy at 1 000 mg/kg/day Genotoxicity Salmonella typhimurium Reverse Mutation Assay with Shell Brake Fluid Dot 4 (Brooks & Wiggins, 1992) Shell Brake Fluid Dot 4 Strains: Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 98, TA 100 and Escherichia coli WP2 uvrA(pKM101) Concentration range: 31.1 – 5 000 g/plate Test method: Maron and Ames (Maron & Ames, 1983): metabolic activation was provided by Aroclor 1254-induced rat liver S9 fraction; positive control compounds to check that the strains exhibited the appropriate responses were benzo(a)pyrene, potassium dichromate, neutral red, sodium azide, 2- nitrofluorene, 2-aminoanthracene and 9- aminoacridine
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