strongest substrate preference; represents the strongest substrate preference or the weakest substrate ω-3 “-10” ω-6 ω-3 preference. b Preference indexes equal [Catalytic efficiencyALA/ Catalytic efficiencyLA] or -[Catalytic efficiencyLA/ Catalytic efficiencyALA]. c data indicated fatty acid (w/w %) content GLA and SDA respectively in corresponding strains. indicated “-” not to catalyze LA or ALA. d The blank spaces represent not mentioned in corresponding reference, but its preference is known, or represent its locus is not found. Based on previous summary of catalytic efficiency of FADS2s from various species with LA and ALA substrates, their catalytic efficiency was reordered according to catalytic efficiency of ALA substrate. Supplemental Table S2 Primers used in this study Restriction Primer name Oligonucleotide sequence (5'-3')a Function enzyme TpFADS6 amplification FTp I TACCGGAATTCATGGGAAAAGGAGGAGACGC EcoR for expression in S. RTp I CGGGGCCTCGAGTTACATGGCAGGGAAATCCT Xho cerevisiae FGc I TACCGGAATTCATGCGTGGATGGTGCACCAC GcFADS6 amplification EcoR for expression in S. RGc I Not cerevisiae T7 - TAATACGACTCACTATAGGG Target genes insert detection for yeast pYES2.R - TCGGTTAGAGCGGATGTG expression construction FA I EcoR TACCGGAATTCATGGGAAAAGGAGGAGACGC RA - FB - RB I Not
strongest ω-3 substrate preference; “-10” represents the strongest ω-6 substrate preference or the weakest ω-3 substrate preference. b Preference indexes equal [Catalytic efficiencyALA/ Catalytic efficiencyLA] or -[Catalytic efficiencyLA/ Catalytic efficiencyALA]. c data indicated fatty acid (w/w %) content GLA and SDA respectively in corresponding strains. “-” indicated not to catalyze LA or ALA. d The blank spaces represent not mentioned in corresponding reference, but its preference is known, or represent its locus is not found. Based on previous summary of catalytic efficiency of FADS2s from various species with LA and ALA substrates, their catalytic efficiency was reordered according to catalytic efficiency of ALA substrate. Supplemental Table S2 Primers used in this study Primer name Restriction enzyme Oligonucleotide sequence (5'-3')a Function FTp EcoR I TACCGGAATTCATGGGAAAAGGAGGAGACGC TpFADS6 amplification for expression in S. cerevisiae RTp Xho I CGGGGCCTCGAGTTACATGGCAGGGAAATCCT FGc EcoR I TACCGGAATTCATGCGTGGATGGTGCACCAC GcFADS6 amplification for expression in S. cerevisiae RGc Not I T7 - TAATACGACTCACTATAGGG Target genes insert detection for yeast expression construction pYES2.R - TCGGTTAGAGCGGATGTG FA EcoR I TACCGGAATTCATGGGAAAAGGAGGAGACGC RA - FB - RB Not I
Molecular%20mechanisms%20underlying%20catalytic%20activity%20of%20delta%206%20desaturaseSI.docx