Performs: Examine media for Risk of aerosol production Moderate Required: Low growth. (See Table 1 for aerosol Aerobic/anaerobic Follow written procedures. Work up cultures as per generating activities) Wear PPE: lab coat, gloves and additional eye bacterial culture High (possible laboratory policy based Risk of contamination by protection when there is a risk of a splash or spray. identification and high risk on specimen source. touching growth Do not touch your face, eyes, nose, or mouth without pathogens) susceptibility Perform AST as per Risk of spill or splash washing your hands first. laboratory policy based when handling liquid testing using Perform all aerosol-generating work in a Class II BSC on specimen source. media when manipulating possible high risk pathogens manual methods: Prepare using BSL-3 practices (controlled access to the area Kirby Bauer standardized when working, decontamination of all waste, must E-test inoculum. wear a solid front gown with cuffed sleeves, gloves Manual MIC Inoculate and N-95 respirator/PAPR). Incubate Manual ID (e.g. Additional Protection Preferred: – Read API 20E, Rapid Wear gloves and additional eye protection when there Discard waste into is a risk of a splash or spray. If wear gloves when Ana ID, spot appropriate handling cultures, establish clean and dirty areas IDs, NF panels, container. and be sure to remove gloves and wash hands etc.) before touching anything in a clean area (i.e. List your test specific process Other (Be telephones, door handles, computer keyboards). steps here. Perform all aerosol-generating work in a Class II BSC. specific) Do not use a flame to heat fix Gram stain slides, but ______________ allow to air dry, or dry on a heating block. _______________ Additional Protection - Alternative: _______________ Perform all aerosol-generating work behind a full safety shield/face shield and wear eye protection. Comments: Culture Work-up: (continued) Procedure Process Step Potential Hazards Initial Risk Control (Mitigation) Residual Level Risk Level
Performs: Aerobic/anaerobic bacterial culture identification and susceptibility testing using manual methods: Kirby Bauer E-test Manual MIC Manual ID (e.g. API 20E, Rapid Ana ID, spot IDs, NF panels, etc.) Other (Be specific) ______________ _______________ _______________ Examine media for growth. Work up cultures as per laboratory policy based on specimen source. Perform AST as per laboratory policy based on specimen source. Prepare standardized inoculum. Inoculate Incubate Read Discard waste into appropriate container. List your test specific process steps here. Risk of aerosol production (See Table 1 for aerosol generating activities) Risk of contamination by touching growth Risk of spill or splash when handling liquid media Moderate High (possible high risk pathogens) Required: Follow written procedures. Wear PPE: lab coat, gloves and additional eye protection when there is a risk of a splash or spray. Do not touch your face, eyes, nose, or mouth without washing your hands first. Perform all aerosol-generating work in a Class II BSC when manipulating possible high risk pathogens using BSL-3 practices (controlled access to the area when working, decontamination of all waste, must wear a solid front gown with cuffed sleeves, gloves and N-95 respirator/PAPR). Additional Protection – Preferred: Wear gloves and additional eye protection when there is a risk of a splash or spray. If wear gloves when handling cultures, establish clean and dirty areas and be sure to remove gloves and wash hands before touching anything in a clean area (i.e. telephones, door handles, computer keyboards). Perform all aerosol-generating work in a Class II BSC. Do not use a flame to heat fix Gram stain slides, but allow to air dry, or dry on a heating block. Additional Protection - Alternative: Perform all aerosol-generating work behind a full safety shield/face shield and wear eye protection. Low Comments: Culture Work-up: (continued) Procedure Process Step Potential Hazards Initial Risk Level Control (Mitigation) Residual Risk Level
171025-Final-Revised-Risk-Assessment-Tool.docx