Camera 1, Camera Preset 2 shows the screen on the GC itself which tells you the current temperature of the column. Figure 13: Camera 1, Camera Preset 2 GC temperature. The Status” field will indicate when the GC has reached the proper pressure and “GC temperature settings. At that point, the Needle” message appears in the GC Status “Inject window. When the Needle” button is clicked, it causes the robot to move the pump “Insert forward and insert the needle into the GC column. Select Camera 2, Camera Preset 2 to view this needle. Figure 14: Select the Needle” button. “Insert After the needle has been inserted, the and Collect” button is available. When this “Input button is selected, the robotic pumping system will deliver the correct amount of sample compound. The needle will be withdrawn from the column automatically. Figure 15: Select the and Collect” button. “Inject Data is now being generated. GC is running data. Figure 16: Viewing the Data Generated by the GC in a Graphic Format (Chromatogram) Select the tab on the lab interface to view the data being generated. At first, the data “Graph” on the chromatogram will be noisy as shown in Figure 17. This will smooth out as soon as the chemicals begin to work their way out of the end of the GC column and impact on the detector. Data is Figure 17: “Noisy” When this happens, you will see a dramatic change in the on the left side of the chromatogram as shown in Figure 18. It often takes 5 to 6 minutes for the run to occur from the beginning to the end of the column. You will see separate peaks for separate chemicals as they exit the chromatograph column. Figure 18: Real data is captured.
Camera 1, Camera Preset 2 shows the screen on the GC itself which tells you the current temperature of the column. Figure 13: Camera 1, Camera Preset 2 GC temperature. The “GC Status” field will indicate when the GC has reached the proper pressure and temperature settings. At that point, the “Inject Needle” message appears in the GC Status window. When the “Insert Needle” button is clicked, it causes the robot to move the pump forward and insert the needle into the GC column. Select Camera 2, Camera Preset 2 to view this needle. Figure 14: Select the “Insert Needle” button. After the needle has been inserted, the “Input and Collect” button is available. When this button is selected, the robotic pumping system will deliver the correct amount of sample compound. The needle will be withdrawn from the column automatically. Figure 15: Select the “Inject and Collect” button. Data is now being generated. Figure 16: GC is running data. Viewing the Data Generated by the GC in a Graphic Format (Chromatogram) Select the “Graph” tab on the lab interface to view the data being generated. At first, the data on the chromatogram will be noisy as shown in Figure 17. This will smooth out as soon as the chemicals begin to work their way out of the end of the GC column and impact on the detector. Figure 17: Data is “Noisy” When this happens, you will see a dramatic change in the on the left side of the chromatogram as shown in Figure 18. It often takes 5 to 6 minutes for the run to occur from the beginning to the end of the column. You will see separate peaks for separate chemicals as they exit the chromatograph column. Figure 18: Real data is captured.
NANSLO_gas_chromatography_lab_activity_with_Checklist.docx