Lab Skills Microscope: Total Magnification = Eyepiece x Objective lens Diaphragm Regulates Light – 1 mm = 1000 (micrometers) μm Objects are viewed upside down and backwards. (iodine or methylene blue) makes it easier to see specimen details. – Slide Preparation: Specimen Staining… ___________________________________________________________________________________ Graduated Cylinder: measures liquid volume READ THE BOTTOM OF THE CURVE (MENISCUS). ___________________________________________________________________________________ Centrifuge spins rapidly and separates (ex. cell parts) according to density. – USE FOR THINGS YOU CAN’T SEE WITH A COMPOUND MICROSCOPE. Microdissecting Instruments can remove cell parts. – ex. chloroplasts, nucleus, mitochondria… USE FOR THINGS YOU CAN’T SEE WITH A COMPOUND MICROSCOPE. ___________________________________________________________________________________
Lab Skills Microscope: Total Magnification = Eyepiece x Objective lens Diaphragm – Regulates Light 1 mm = 1000 μm (micrometers) Objects are viewed upside down and backwards. (iodine or methylene blue) – makes it easier to see specimen details. Slide Preparation: Specimen Staining… ___________________________________________________________________________________ Graduated Cylinder: measures liquid volume READ THE BOTTOM OF THE CURVE (MENISCUS). ___________________________________________________________________________________ Centrifuge – spins rapidly and separates (ex. cell parts) according to density. USE FOR THINGS YOU CAN’T SEE WITH A COMPOUND MICROSCOPE. Microdissecting Instruments – can remove cell parts. ex. chloroplasts, nucleus, mitochondria… USE FOR THINGS YOU CAN’T SEE WITH A COMPOUND MICROSCOPE. ___________________________________________________________________________________
mid2017.docx