9.1 Acute Toxicity 9.1.1 Acute Oral Toxicity 9.1.1.1 Acute Oral Toxicity of C12-C16 Alpha Olefin (Rinehart 1967) C12-C16 alpha Olefin (98.5% olefins, 1.5% saturates) Test Substance: Rat/Wistar Species/strain: 10 males Number/sex of animals: 14 days Observation period: Gavage 5 000 mg/kg or 10 000 mg/kg (dose volume of 2.65 Method of administration: 3.50 mL/kg). – US Federal Hazardous Substances Labelling Act Test method: A few days after dosing, animals had very coarse oily fur Clinical observations: over the whole body, particularly severe in the hindquarter area. This observation persisted for the remainder of the observation period. Two deaths observed in the 10 000 mg/kg dose; considered Mortality: to be due to pneumonia. Not reported Morphological findings: > 5 000 mg/kg LD50: C12-C16 Alpha Olefin was of very low acute oral toxicity in Result: rats. 9.1.1.2 Acute Oral Toxicity of C16 Alpha Olefin (Rinehart 1967) C16 alpha Olefin (98.5% olefins, 1.5% saturates) Test Substance: Rat/Wistar Species/strain: 10 males Number/sex of animals: 14 days Observation period: Oral, gavage 5 000 mg/kg or 10 000 mg/kg (dose volume of Method of administration: 2.65 3.50 mL/kg). – US Federal Hazardous Substances Labelling Act. Test method:
9.1 Acute Toxicity 9.1.1 Acute Oral Toxicity 9.1.1.1 Acute Oral Toxicity of C12-C16 Alpha Olefin (Rinehart 1967) Test Substance: C12-C16 alpha Olefin (98.5% olefins, 1.5% saturates) Species/strain: Rat/Wistar Number/sex of animals: 10 males Observation period: 14 days Method of administration: Gavage 5 000 mg/kg or 10 000 mg/kg (dose volume of 2.65 – 3.50 mL/kg). Test method: US Federal Hazardous Substances Labelling Act Clinical observations: A few days after dosing, animals had very coarse oily fur over the whole body, particularly severe in the hindquarter area. This observation persisted for the remainder of the observation period. Mortality: Two deaths observed in the 10 000 mg/kg dose; considered to be due to pneumonia. Morphological findings: Not reported LD50: > 5 000 mg/kg Result: C12-C16 Alpha Olefin was of very low acute oral toxicity in rats. 9.1.1.2 Acute Oral Toxicity of C16 Alpha Olefin (Rinehart 1967) Test Substance: C16 alpha Olefin (98.5% olefins, 1.5% saturates) Species/strain: Rat/Wistar Number/sex of animals: 10 males Observation period: 14 days Method of administration: Oral, gavage 5 000 mg/kg or 10 000 mg/kg (dose volume of 2.65 – 3.50 mL/kg). Test method: US Federal Hazardous Substances Labelling Act.
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