Origin of Insert Name of Vector(s) Name of Host(s) Gene of Interest If an attempt will be DNA/RNA Used (plasmid or Used made to obtain virus/strain) (bacteria/strain, expression of a virus/strain or cells) foreign gene, indicate the specific List each step of the protein that will be cloning process List each step of the produced cloning process, including step (E. coli, etc.) Answer the following questions. Yes a. Does the inserted gene encode a known oncogene? No Yes b. Does the viral DNA integrate into the host genome? No Yes No c. Does the inserted gene have the potential for altering the cell cycle? Yes No d. Is there a probability of generating replication-competent viruses? If yes to any of the above, please explain: Exempt Experiments. The following recombinant or synthetic nucleic acid molecules are exempt from the NIH Guidelines and registration with the Institutional Biosafety Committee (IBC) is not required. Will the experiment include synthetic nucleic acids that: Can either replicate or generate nucleic that can replicate in any Yes living cell? No Yes Are designed to integrate into DNA? No
Origin of Insert DNA/RNA Name of Vector(s) Used (plasmid or virus/strain) List each step of the cloning process Name of Host(s) Used (bacteria/strain, virus/strain or cells) List each step of the cloning process, including step (E. coli, etc.) Gene of Interest If an attempt will be made to obtain expression of a foreign gene, indicate the specific protein that will be produced Answer the following questions. a. Does the inserted gene encode a known oncogene? Yes No b. Does the viral DNA integrate into the host genome? Yes No c. Does the inserted gene have the potential for altering the cell cycle? Yes No d. Is there a probability of generating replication-competent viruses? Yes No If yes to any of the above, please explain: Exempt Experiments. The following recombinant or synthetic nucleic acid molecules are exempt from the NIH Guidelines and registration with the Institutional Biosafety Committee (IBC) is not required. Will the experiment include synthetic nucleic acids that: Can either replicate or generate nucleic that can replicate in any living cell? Yes No Are designed to integrate into DNA? Yes No
NOU%20Amendment-%20Recombinant%20materials%205.27.2016.docx