Test and Control animals: Challenge procedure: Day 21: A 24 hour, semi occluded application of 100% w/v of test substance to the right flank of each animal, and 75% w/v test substance in dried arachis oil, to the left flank of each animal. No skin reactions were observed in test animals at the 24 or Challenge Outcome: 48-hour observation period. Grade 1 erythema (discrete or patchy erythema) was observed in one control animal at the 24 hour observation period. No other reactions were noted in control animals. Alkane 2 was non-sensitising to guineapig skin. Result: 9.1.6.2 Skin Sensitisation in Guineapigs [Hill Top Biolabs Inc, 1995 #18] Guineapig/Hartley Species/strain: 10/sex (test 5/sex (naïve control group) Number of animals: OECD TG 406 Buehler Technique Test method: Maximum Concentration Irritancy was observed at 0.5%, the lowest concentration not giving rise to irritating tested. effects in irritation screen: test animals: Induction procedure: Days 1, 7 and 14: 0.3 mL of neat test substance applied, via a Hill Top Chamber, to the clipped skin of the left shoulder for 6 hours; test and naïve control animals: Challenge procedure: Day 28: same procedure as induction phase, except test substance was applied to a previously non-treated site; Grading of dermal responses occurred 24 and 48 hours post exposure Number of animals exhibiting positive responses 1 or more) at challenge: Test Animals Naïve Control Animals Challenge concentration 24 hours* 48 hours* 24 hours* 48 hours* 100% 3/19** 7/19 1/10 3/10 * time after patch removal. ** one female was euthanised at induction because of uncertainity of pregnancy status. Following primary challenge the incidence and severity of Challenge outcome: the responses 1 slight but confluent, or moderate –
Challenge procedure: Test and Control animals: Day 21: A 24 hour, semi occluded application of 100% w/v of test substance to the right flank of each animal, and 75% w/v test substance in dried arachis oil, to the left flank of each animal. Challenge Outcome: No skin reactions were observed in test animals at the 24 or 48-hour observation period. Grade 1 erythema (discrete or patchy erythema) was observed in one control animal at the 24 hour observation period. No other reactions were noted in control animals. Result: Alkane 2 was non-sensitising to guineapig skin. 9.1.6.2 Skin Sensitisation in Guineapigs [Hill Top Biolabs Inc, 1995 #18] Species/strain: Guineapig/Hartley Number of animals: 10/sex (test 5/sex (naïve control group) Test method: OECD TG 406 Buehler Technique Maximum Concentration not giving rise to irritating effects in irritation screen: Irritancy was observed at 0.5%, the lowest concentration tested. Induction procedure: test animals: Days 1, 7 and 14: 0.3 mL of neat test substance applied, via a Hill Top Chamber, to the clipped skin of the left shoulder for 6 hours; Challenge procedure: test and naïve control animals: Day 28: same procedure as induction phase, except test substance was applied to a previously non-treated site; Grading of dermal responses occurred 24 and 48 hours post exposure Number of animals exhibiting positive responses 1 or more) at challenge: Challenge Test Animals Naïve Control Animals concentration 24 hours* 48 hours* 24 hours* 48 hours* 100% 3/19** 7/19 1/10 3/10 * time after patch removal. ** one female was euthanised at induction because of uncertainity of pregnancy status. Challenge outcome: Following primary challenge the incidence and severity of the responses 1 – slight but confluent, or moderate
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