72 hours Observation period: A volume of 0.1 mL of the notified chemical was placed into Method of administration: the everted lid of one eye of each animal. The other eye served as control. OECD TG 405 Test method: Draize scores: Time instillation Animal 1 hour 1 day 2 days 3 days Conjunctiva r c r c r c r c 1 2 0 2 0 0 0 0 0 2 1 0 1 0 0 0 0 0 3 1 0 1 0 0 0 0 0 4 1 0 1 0 0 0 0 0 5 1 0 1 0 0 0 0 0 6 1 0 0 0 0 0 0 0 1 see Attachment 1 for Draize scales r = redness c = chemosis Draize scores for cornea (density and area) and iris lesion in Other Draize scores: all 6 animals were zero during the study. Discharge from conjunctivae was not recorded. Comment: The notified chemical was a slight irritant to the eyes of Result: rabbits. 9.1.6 Skin Sensitisation (Coleman, 1998) Guinea pigs/albino Dunkin- Hartley Species/strain: 20 (test group); Number of animals: 20 males (test vehicle group); 10 males (positive control group); 10 males (vehicle control group). 3 pairs of intradermal injections (0.1 mL) were made on Induction: Intradermal shaved shoulder area of each animal: injection (Day 1) Test Freund’s Complete Adjuvant (FCA) with water (1:1 v/v);
Observation period: 72 hours Method of administration: A volume of 0.1 mL of the notified chemical was placed into the everted lid of one eye of each animal. The other eye served as control. Test method: OECD TG 405 Draize scores: Time instillation Animal 1 hour 1 day 2 days 3 days Conjunctiva r c r c r c r c 1 2 0 2 0 0 0 0 0 2 1 0 1 0 0 0 0 0 3 1 0 1 0 0 0 0 0 4 1 0 1 0 0 0 0 0 5 1 0 1 0 0 0 0 0 6 1 0 0 0 0 0 0 0 1 see Attachment 1 for Draize scales r = redness c = chemosis Other Draize scores: Draize scores for cornea (density and area) and iris lesion in all 6 animals were zero during the study. Comment: Discharge from conjunctivae was not recorded. Result: The notified chemical was a slight irritant to the eyes of rabbits. 9.1.6 Skin Sensitisation (Coleman, 1998) Species/strain: Guinea pigs/albino Dunkin- Hartley Number of animals: 20 (test group); 20 males (test vehicle group); 10 males (positive control group); 10 males (vehicle control group). Induction: Intradermal injection (Day 1) 3 pairs of intradermal injections (0.1 mL) were made on shaved shoulder area of each animal: Test Freund’s Complete Adjuvant (FCA) with water (1:1 v/v);
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