31 females, 5 Number/sex of volunteers: Each volunteer received 0.2 mL (25% dilution in mineral oil) Induction procedure: of test substance in a semi occluded exposure of 24 hours on 3 alternate days for weeks (9 induction exposures). A rest period of 10 17 days followed. – Sodium lauryl sulphate (0.1% in water) served as the control. Each volunteer received 0.2 mL (25% dilution in mineral oil) Challenge procedure: of test substance in a semi occluded patch exposure to a naïve site on the upper arm for 24 hours. No reaction to the to the test substance was reported. Challenge Outcome: Neodene 18 olefin was reported non sensitising to Result: human skin 9.2 Repeat Dose Toxicity 9.2.1 Repeated Dose Dermal Toxicity: 2 Week Using Gulftene 12-16 (Gulf Life Sciences Center 1983) Gulftene 12-16 Test Substance: Rat/Fischer 344 Species/strain: 5/sex/group Number/sex of animals: Doses of 0, 1.0, 2.0 g/kg applied once a day for a 6 hour duration: exposure period, for a total of 9 doses over a 2-week period 2.60 mL/kg of test substance applied to a clipped site of the Method of administration: back. An Elizabethan collar prevented animals interfering with the dose site. The vehicle was corn oil. OECD TG 410 Test method: Nil Mortality: Clinical observations: Body weight gains were depressed in the 2.0 g/kg group. No test related toxic signs or symptoms were observed. Dermal Reactions: Individual Draize scores not provided. 1 000 mg/kg group:
Number/sex of volunteers: 31 females, 5 Induction procedure: Each volunteer received 0.2 mL (25% dilution in mineral oil) of test substance in a semi occluded exposure of 24 hours on 3 alternate days for weeks (9 induction exposures). A rest period of 10 – 17 days followed. Sodium lauryl sulphate (0.1% in water) served as the control. Challenge procedure: Each volunteer received 0.2 mL (25% dilution in mineral oil) of test substance in a semi occluded patch exposure to a naïve site on the upper arm for 24 hours. Challenge Outcome: No reaction to the to the test substance was reported. Result: Neodene 18 olefin was reported non sensitising to human skin 9.2 Repeat Dose Toxicity 9.2.1 Repeated Dose Dermal Toxicity: 2 Week Using Gulftene 12-16 (Gulf Life Sciences Center 1983) Test Substance: Gulftene 12-16 Species/strain: Rat/Fischer 344 Number/sex of animals: 5/sex/group duration: Doses of 0, 1.0, 2.0 g/kg applied once a day for a 6 hour exposure period, for a total of 9 doses over a 2-week period Method of administration: 2.60 mL/kg of test substance applied to a clipped site of the back. An Elizabethan collar prevented animals interfering with the dose site. The vehicle was corn oil. Test method: OECD TG 410 Mortality: Nil Clinical observations: Body weight gains were depressed in the 2.0 g/kg group. No test related toxic signs or symptoms were observed. Dermal Reactions: Individual Draize scores not provided. 1 000 mg/kg group:
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