guinea pig/Ibm: GOHI SPF Species/strain: 10 males test group, 5 males control group Number/sex of animals: Induction procedure: test group: day 1 to a clipped area of the scapular dorsal skin, each animal received 3 pairs of 0.1 mL injections as follows – 1:1 (v/v) mixture of Freund’s Complete Adjuvant and physiological saline the test material diluted to 5 % in bi-distilled water the test material diluted to 5 % by emulsion with 1:1 (v/v) mixture of Freund’s Complete Adjuvant and physiological saline day 8 a filter paper patch with 0.3 g of test material (50 % (w/v) in bi-distilled water) was placed over the injection area; covered with aluminium foil and secured with elastic plaster and impervious adhesive tape control the induction procedure was identical to that for the test group, except that water only was used in place of the aqueous solution of test material in both induction phases Challenge procedure: day 22 a 24 hour occluded application of 0.2 mL of a 25 % solution of the test material was applied to the shaved flank of both test and control animals OECD TG 406 Test method: no skin response to the challenge application of test material Comment: was observed in either the test or control animals the notified chemical was not sensitising to the skin of Result: guinea pigs 9.2 Repeated Dose Toxicity (Allard et al., 1997) rat/HanIbm: WIST (SPF) Species/strain: group 1: 10/sex Number/sex of animals: 2: 5/sex group 3: 5/sex group 4: 10/sex
Species/strain: guinea pig/Ibm: GOHI SPF Number/sex of animals: 10 males test group, 5 males control group Induction procedure: test group: day 1 day 8 to a clipped area of the scapular dorsal skin, each animal received 3 pairs of 0.1 mL injections as follows – 1:1 (v/v) mixture of Freund’s Complete Adjuvant and physiological saline the test material diluted to 5 % in bi-distilled water the test material diluted to 5 % by emulsion with 1:1 (v/v) mixture of Freund’s Complete Adjuvant and physiological saline a filter paper patch with 0.3 g of test material (50 % (w/v) in bi-distilled water) was placed over the injection area; covered with aluminium foil and secured with elastic plaster and impervious adhesive tape control the induction procedure was identical to that for the test group, except that water only was used in place of the aqueous solution of test material in both induction phases Challenge procedure: day 22 a 24 hour occluded application of 0.2 mL of a 25 % solution of the test material was applied to the shaved flank of both test and control animals Test method: OECD TG 406 Comment: no skin response to the challenge application of test material was observed in either the test or control animals Result: the notified chemical was not sensitising to the skin of guinea pigs 9.2 Repeated Dose Toxicity (Allard et al., 1997) Species/strain: rat/HanIbm: WIST (SPF) Number/sex of animals: group 1: 10/sex 2: 5/sex group 3: 5/sex group 4: 10/sex
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