Rat/Sprague-Dawley (CD) Species/strain: 5/sex per group Number/sex of animals: 15 days Observation period: Oral (gavage) doses at 2 000, 3 200, 5 000 and 6 400 mg/kg. Method of administration: OECD TG 401 Test method: 1 male at 3 200 mg/kg; Mortality: 1 male and 3 females at 5 000 mg/kg; and 3 at 6 400 mg/kg. Piloerection, hunched posture, red brown stained muzzle, Clinical observations: ungroomed appearance and walking on toes were seen in most animals at all dose levels. Increased salivation, waddling/unsteady gait, lethargy, sensitivity to handling, respiratory distress, abnormal faeces, cold extremities, body tremors, increased lacrimation, prostration, thin appearance, pallid extremities, protruding eyes and partially close eyelids were observed at one or more dosages. All symptoms reversed by day 14 except piloerection, which was still evident on day 15 in males at 5000 mg/kg. A generalised congestion was observed in either all or the Morphological findings: majority of organs and tissues of the dead animals. No macroscopic abnormalities were found in surviving animals. All deaths (1 male was killed occurred within 3 Comment: in extremis) days of dosing. 5 537 mg/kg (males), LD50: 4 938 mg/kg (females), 5 313 mg/kg (combined The notified chemical was of very low acute oral toxicity in Result: rats. 9.1.2 Dermal Toxicity (McRae, 1998a) Rat/Sprague-Dawley(CD) Species/strain: 5/sex Number/sex of animals: 15 days Observation period:
Species/strain: Rat/Sprague-Dawley (CD) Number/sex of animals: 5/sex per group Observation period: 15 days Method of administration: Oral (gavage) doses at 2 000, 3 200, 5 000 and 6 400 mg/kg. Test method: OECD TG 401 Mortality: 1 male at 3 200 mg/kg; 1 male and 3 females at 5 000 mg/kg; and 3 at 6 400 mg/kg. Clinical observations: Piloerection, hunched posture, red brown stained muzzle, ungroomed appearance and walking on toes were seen in most animals at all dose levels. Increased salivation, waddling/unsteady gait, lethargy, sensitivity to handling, respiratory distress, abnormal faeces, cold extremities, body tremors, increased lacrimation, prostration, thin appearance, pallid extremities, protruding eyes and partially close eyelids were observed at one or more dosages. All symptoms reversed by day 14 except piloerection, which was still evident on day 15 in males at 5000 mg/kg. Morphological findings: A generalised congestion was observed in either all or the majority of organs and tissues of the dead animals. No macroscopic abnormalities were found in surviving animals. Comment: All deaths (1 male was killed in extremis) occurred within 3 days of dosing. LD50: 5 537 mg/kg (males), 4 938 mg/kg (females), 5 313 mg/kg (combined Result: The notified chemical was of very low acute oral toxicity in rats. 9.1.2 Dermal Toxicity (McRae, 1998a) Species/strain: Rat/Sprague-Dawley(CD) Number/sex of animals: 5/sex Observation period: 15 days
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