A few days dosing, animals had very coarse oily fur Clinical observations: over the whole body, particularly severe in the hindquarter area; Animals developed a weakness in the hindquarters which caused them to drag around the cage. This observation persisted for the remainder of the observation period. Nil Mortality: Not reported. Morphological findings: > 5 000 mg/kg LD50: C16 Olefin was of very low acute oral toxicity in rats. Result: 9.1.1.3 Oral Toxicity of C16 Isomerised Olefin (Hill Top Biolabs Inc 1993) C16 Isomerised Olefin Test Substance: Rat/Sprague Dawley Species/strain: 5/sex Number/sex of animals: 14 days Observation period: Oral, gavage 5 050 mg/kg (dose volume of 5.92 mL/kg) Method of administration: OECD TG 420 fixed dose method Test method: – One female lost weight between Days 0 and 7 and one Clinical observations: female failed to gain weight between days 7 and 14. Prominent in life observations included activity decrease piloerection and polyuria, which were no longer evident by Day 7. Alopecia was observed in all animals on Days 7 through 14. Nil Mortality: No abnormalities detected. Morphological findings: > 5 050 mg/kg LD50: C16 Isomerised Olefin was of very low acute oral toxicity in Result: rats. 9.1.1.4 Acute Oral Toxicity of C18 Isomerised Olefin (Stillmeadow Inc 1993) C18 Isomerised Olefin Test Substance:
Clinical observations: A few days dosing, animals had very coarse oily fur over the whole body, particularly severe in the hindquarter area; Animals developed a weakness in the hindquarters which caused them to drag around the cage. This observation persisted for the remainder of the observation period. Mortality: Nil Morphological findings: Not reported. LD50: > 5 000 mg/kg Result: C16 Olefin was of very low acute oral toxicity in rats. 9.1.1.3 Oral Toxicity of C16 Isomerised Olefin (Hill Top Biolabs Inc 1993) Test Substance: C16 Isomerised Olefin Species/strain: Rat/Sprague Dawley Number/sex of animals: 5/sex Observation period: 14 days Method of administration: Oral, gavage 5 050 mg/kg (dose volume of 5.92 mL/kg) Test method: OECD TG 420 – fixed dose method Clinical observations: One female lost weight between Days 0 and 7 and one female failed to gain weight between days 7 and 14. Prominent in life observations included activity decrease piloerection and polyuria, which were no longer evident by Day 7. Alopecia was observed in all animals on Days 7 through 14. Mortality: Nil Morphological findings: No abnormalities detected. LD50: > 5 050 mg/kg Result: C16 Isomerised Olefin was of very low acute oral toxicity in rats. 9.1.1.4 Acute Oral Toxicity of C18 Isomerised Olefin (Stillmeadow Inc 1993) Test Substance: C18 Isomerised Olefin
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