Reagent 4 Test Your station contains a set of 5 Cell Type Samples (A-E). It also contains a set of Cell Type Controls, for comparison. Set aside the Controls. Add 5 drops of Reagent 4 to each test tube. GENTLY shake/swirl the contents of each test tube. CAUTION!!! This Reagent will stain both your skin and your clothing. Use your Cell Type Controls to compare the color of the test tubes containing Reagent 4. Reagent 4 is positive if it changes from yellow to brown color. In the Data Table, write for the presence of brown solution, or if the solution stays yellow or clear. “+” “-“ 8. Obtain/prepare 50 mL of Unknown Cell Type Solution. Consider using the following: 2% milk, half and half, peanut butter solution, clear sports drink, chicken liver broth/solution 9. Place 5 mL of the Unknown into each of four labeled test tubes. 10. Determine and how class data will be collected, (i.e. projected on board, inserted into Google Doc Table, spreadsheet, etc). 11. Print one copy of Modeling Cell Labeling to Identify Cell Types Lab Sheet for each student (found at end of this document). 12. Cue and preview Pre-Lab video. Post-Lab Discussion: Discuss group and class data. Ask students to write and discuss conclusions that can be drawn from both. Extension and Follow-up Activity: Post-Lab Follow-Up: Jennifer West Additional Video Resources: Video: Source: Adapted from
Reagent 4 Test Your station contains a set of 5 Cell Type Samples (A-E). It also contains a set of Cell Type Controls, for comparison. Set aside the Controls. Add 5 drops of Reagent 4 to each test tube. GENTLY shake/swirl the contents of each test tube. CAUTION!!! This Reagent will stain both your skin and your clothing. Use your Cell Type Controls to compare the color of the test tubes containing Reagent 4. Reagent 4 is positive if it changes from yellow to brown color. In the Data Table, write “+” for the presence of brown solution, or “-“ if the solution stays yellow or clear. 8. Obtain/prepare 50 mL of Unknown Cell Type Solution. Consider using the following: 2% milk, half and half, peanut butter solution, clear sports drink, chicken liver broth/solution 9. Place 5 mL of the Unknown into each of four labeled test tubes. 10. Determine and how class data will be collected, (i.e. projected on board, inserted into Google Doc Table, spreadsheet, etc). 11. Print one copy of Modeling Cell Labeling to Identify Cell Types Lab Sheet for each student (found at end of this document). 12. Cue and preview Pre-Lab video. Post-Lab Discussion: Discuss group and class data. Ask students to write and discuss conclusions that can be drawn from both. Extension and Follow-up Activity: Post-Lab Follow-Up: Jennifer West Additional Video Resources: Video: Source: Adapted from
ModelingCellLabelingtoIdentifyCellTypesLessonPlan.docx