(v/v); distilled water; adjuvant/saline 1:1 (v:v) mixture with distilled water(w/w) 1:1. Induction at day 8: test topical: 50% notified chemical in distilled water; control group distilled water only Challenge at day 22: test and control group 1, 50% notified chemical in distilled water and distilled water only. Re-challenge at day 29: test and control group 2, 15 and 25% notified chemical in distilled water The Challenge Outcome: Challenge 24 hrs 48hrs Concentration test control test control 0% 0/20 0/10 0/20 0/10 25% 14/20 *4/10 13/20 4/10 * 50% concentration of FAT’530/A showed an irritancy potential of 40% on the control group 1. A second challenge was performed with two lower concentrations, 15% and 25% on the same test group but using a new control group (control group 2) Re-challenge 24 hrs 48hrs Concentration test control test control 0% 3/20 0/10 0/20 0/10 25% 7/20 0/10 6/20 0/10 Test Method: Directive 92/69/EEC (5) test B6 (27) Result: skin sensitiser in the guinea 9.1.6.2 Test 2 (13) Species/strain: Himalayan spotted guinea pig Number of animals: 20 in test group, female guinea pigs/10 in control groups (Ibm: GOHI) Induction at day 1: test group: injections of adjuvant/saline mixture 1:1 (v/v); 5% notified chemical in distilled water; 5% notified chemical in the
(v/v); distilled water; adjuvant/saline 1:1 (v:v) mixture with distilled water(w/w) 1:1. Induction at day 8: test topical: 50% notified chemical in distilled water; control group distilled water only Challenge at day 22: test and control group 1, 50% notified chemical in distilled water and distilled water only. Re-challenge at day 29: test and control group 2, 15 and 25% notified chemical in distilled water The Challenge Outcome: Challenge Concentration 24 hrs 48hrs test control test control 0% 0/20 0/10 0/20 0/10 25% 14/20 *4/10 13/20 4/10 * 50% concentration of FAT’530/A showed an irritancy potential of 40% on the control group 1. A second challenge was performed with two lower concentrations, 15% and 25% on the same test group but using a new control group (control group 2) Re-challenge Concentration 24 hrs 48hrs test control test control 0% 3/20 0/10 0/20 0/10 25% 7/20 0/10 6/20 0/10 Test Method: Directive 92/69/EEC (5) test B6 (27) Result: skin sensitiser in the guinea 9.1.6.2 Test 2 (13) Species/strain: Himalayan spotted guinea pig Number of animals: 20 in test group, female guinea pigs/10 in control groups (Ibm: GOHI) Induction at day 1: test group: injections of adjuvant/saline mixture 1:1 (v/v); 5% notified chemical in distilled water; 5% notified chemical in the
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