Animal 1 hour 1 day 2 days 3 days Conjunctiva r c r c r c r c 1 2 0 0 0 0 0 0 12 2 2 2 0 0 0 0 0 0 3 2 3 0 0 0 0 0 0 1 see Attachment 1 for Draize scales r = redness c = chemosis Draize scores for cornea opacity and iris were all zero at 1, Draize Scores for cornea 24, 48 and 72 hours after treatment. and iris: No staining of the cornea or sclera of the treated eyes by the Comment: notified chemical was observed. Red staining of the conjunctivae was after 1 hour, but had disappeared by 24 hours. the notified chemical was very slightly irritating to the eyes Result: of rabbits 9.1.6 Skin Sensitisation (Arcelin, 1995c) Guinea pigs/Ibm:GOHI;SPF Species/strain: 30 females and 6 males in 4 groups: Number of animals: control 10; test 20; intradermal pretest: 2; epidermal pretest: 4. Induction procedure: test group: day 1 3 of intradermal injections (0.1 mL/site) were made: 1:1 (v/v) mixture of Freund’s Complete Adjuvant (FCA) and physiological saline; The notified chemical, diluted to 5% with water; The notified chemical, diluted to 5% by emulsion in a 1:1 (v/v) mixture of FCA and physiological saline. day 8 A filter paper saturated with the notified chemical (25% aqueous solution) was placed over the injection sites for 48 hours under an occlusive dressing. control group: day 1 3 pairs of intradermal injections (0.1 mL/site) were made: 1) 1:1 (v/v) mixture of FCA and physiological saline; 2) bi-distilled water; 3) 1:1 (v/v) mixture of FCA and physiological saline. day 8
Animal 1 hour 1 day 2 days 3 days Conjunctiva r c r c r c r c 1 12 2 0 0 0 0 0 0 2 2 2 0 0 0 0 0 0 3 2 3 0 0 0 0 0 0 1 see Attachment 1 for Draize scales r = redness c = chemosis Draize Scores for cornea and iris: Draize scores for cornea opacity and iris were all zero at 1, 24, 48 and 72 hours after treatment. Comment: No staining of the cornea or sclera of the treated eyes by the notified chemical was observed. Red staining of the conjunctivae was after 1 hour, but had disappeared by 24 hours. Result: the notified chemical was very slightly irritating to the eyes of rabbits 9.1.6 Skin Sensitisation (Arcelin, 1995c) Species/strain: Guinea pigs/Ibm:GOHI;SPF Number of animals: 30 females and 6 males in 4 groups: control 10; test 20; intradermal pretest: 2; epidermal pretest: 4. Induction procedure: test group: day 1 day 8 3 of intradermal injections (0.1 mL/site) were made: 1:1 (v/v) mixture of Freund’s Complete Adjuvant (FCA) and physiological saline; The notified chemical, diluted to 5% with water; The notified chemical, diluted to 5% by emulsion in a 1:1 (v/v) mixture of FCA and physiological saline. A filter paper saturated with the notified chemical (25% aqueous solution) was placed over the injection sites for 48 hours under an occlusive dressing. control group: day 1 day 8 3 pairs of intradermal injections (0.1 mL/site) were made: 1) 1:1 (v/v) mixture of FCA and physiological saline; 2) bi-distilled water; 3) 1:1 (v/v) mixture of FCA and physiological saline.
NA702FR.docx