Supplementary Figures Nanoneedles integrity following imprinting. SEM imaging of Figure S1. nanoneedles pressed against animal tissue show their substantial integrity. The nanoneedles were pressed against: (a) skin on the back, (b) skin on the ear, (c-d) muscle. Scale bars: (a) 2 (b) 200 nm, (c-d) 1 m, m. Figure S2. Compression testing of nanoneedles. SEM micrographs of nanoneedles subjected to increasing compressive forces. The peak force exerted on the nanoneedle chip is indicated at the top of each panel. Scale bars 2 m. Figure S3. Volumetric Loading of siRNA. (a) Confocal microscopy z-stack of Cy3 fluorescent siRNA (green) loaded throughout the porous volume of pSi nanoneedles. (b) For comparison siRNA only loads over the surface of solidified nanoneedles. Scale bars 2m. Figure S4. Sustained release of siRNA from pSi nanoneedles in PBS. Quantification of siRNA release kinetics from nanoneedles over 24 hours. Figure S5. delivery through nanoneedles. Confocal microscopy z-stacks of nanoneedles interfaced with cells, showing cytosolic localization of siRNA. nN-B show siRNA delivery at 24 hours following interfacing, while nN-T show siRNA delivery at 30 minutes following interfacing. Cells stained with Celltracker Green (green) and fluorescent Cy-3-siRNA (yellow). Scale bars 10 m. Figure S6. nN-T interfacing without application of does not induce intracellular delivery of Cy3-siRNA. Confocal micrographs comparing nN-T interfacing without application of force (a, b) and with application of 100rcf force by centrifugation (c, d) at 30 minutes (a, c) and 4 hours (b, d) following initial interfacing. During the entire duration of the experiment the cells were
Supplementary Figures Figure S1. Nanoneedles integrity following imprinting. SEM imaging of nanoneedles pressed against animal tissue show their substantial integrity. The nanoneedles were pressed against: (a) skin on the back, (b) skin on the ear, (c-d) muscle. Scale bars: (a) 2 m, (b) 200 nm, (c-d) 1 m. Figure S2. Compression testing of nanoneedles. SEM micrographs of nanoneedles subjected to increasing compressive forces. The peak force exerted on the nanoneedle chip is indicated at the top of each panel. Scale bars 2 m. Figure S3. Volumetric Loading of siRNA. (a) Confocal microscopy z-stack of Cy3 fluorescent siRNA (green) loaded throughout the porous volume of pSi nanoneedles. (b) For comparison siRNA only loads over the surface of solidified nanoneedles. Scale bars 2m. Figure S4. Sustained release of siRNA from pSi nanoneedles in PBS. Quantification of siRNA release kinetics from nanoneedles over 24 hours. Figure S5. delivery through nanoneedles. Confocal microscopy z-stacks of nanoneedles interfaced with cells, showing cytosolic localization of siRNA. nN-B show siRNA delivery at 24 hours following interfacing, while nN-T show siRNA delivery at 30 minutes following interfacing. Cells stained with Celltracker Green (green) and fluorescent Cy-3-siRNA (yellow). Scale bars 10 m. Figure S6. nN-T interfacing without application of does not induce intracellular delivery of Cy3-siRNA. Confocal micrographs comparing nN-T interfacing without application of force (a, b) and with application of 100rcf force by centrifugation (c, d) at 30 minutes (a, c) and 4 hours (b, d) following initial interfacing. During the entire duration of the experiment the cells were
Nat.%20Mater%20Nanoneedles%20Supplementary%20Material_2.docx