C18 C30* 0.0 0.0 0.5 0.6 – Olefin/AlcoholH18 *Purity not specified. ** Individual scores not given. H14 (EU 1995) H16 (EU 1995) H18 (EU 1995) 9.1.7 Skin Sensitisation 9.1.7.1 Skin Sensitisation of C12-C16 Alpha Olefins (Rinehart 1967) C12 C16 alpha olefin (98.5% olefins, 1.5% saturates) Test substance: – Guineapig/albino Species/strain: 10 males/group Number of animals: Landsteiner technique in (Rinehart 1967), 1967 #29]) Test method: Test sites on the backs of animals were clipped and scored Induction procedure: with a needle. 0.1mL of test substance was applied by glass rod to the test site three times weekly for nine applications. Positive control: 0.5% chlorodinitrobenzene in 50% ethyl alcohol; Vehicle control: 50% alcohol. Following a 2 week rest period after the last induction Challenge procedure: application 0.1 mL of test article was applied to the skin No dermal response with test substance. Challenge Outcome: The positive control, chlorodinitrobenzene responded appropriately. C12 C16 alpha olefins was non sensitising to guineapig Result: – skin. 9.1.7.2 Skin Sensitisation of C16/C18 Alpha olefins, isomerised (Hill Top Biolabs Inc 1995) C16/C18 Alpha olefins, isomerised Test substance: Guineapig/Hartley White Species/strain: 20 test animals Number of animals: 10 primary challenge naïve control animals; 10 rechallenge naïve control animals
C18 – C30* Olefin/AlcoholH18 0.0 0.0 0.5 0.6 *Purity not specified. ** Individual scores not given. H14 (EU 1995) H16 (EU 1995) H18 (EU 1995) 9.1.7 Skin Sensitisation 9.1.7.1 Skin Sensitisation of C12-C16 Alpha Olefins (Rinehart 1967) Test substance: C12 – C16 alpha olefin (98.5% olefins, 1.5% saturates) Species/strain: Guineapig/albino Number of animals: 10 males/group Test method: Landsteiner technique in (Rinehart 1967), 1967 #29]) Induction procedure: Test sites on the backs of animals were clipped and scored with a needle. 0.1mL of test substance was applied by glass rod to the test site three times weekly for nine applications. Positive control: 0.5% chlorodinitrobenzene in 50% ethyl alcohol; Vehicle control: 50% alcohol. Challenge procedure: Following a 2 week rest period after the last induction application 0.1 mL of test article was applied to the skin Challenge Outcome: No dermal response with test substance. The positive control, chlorodinitrobenzene responded appropriately. Result: C12 – C16 alpha olefins was non sensitising to guineapig skin. 9.1.7.2 Skin Sensitisation of C16/C18 Alpha olefins, isomerised (Hill Top Biolabs Inc 1995) Test substance: C16/C18 Alpha olefins, isomerised Species/strain: Guineapig/Hartley White Number of animals: 20 test animals 10 primary challenge naïve control animals; 10 rechallenge naïve control animals
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