concentration 24 hours* 48 hours* 24 48 25% 0/20** 0/20 0/10 0/10 50% 0/20 0/20 0/10 0/10 * time after patch removal ** number of animals exhibiting positive response Buehler delayed contact hypersensitivity study Test method: based on OECD TG 406 (Organisation for Economic Co-operation and Development, M32F was not a skin sensitiser when tested in Result: guinea pigs Reverse Mutation Assay on M32F-Y (Thompson, Salmonella typhimurium 1996a) TA1535, TA1537, TA1538, TA98 Strains: S. typhimurium and TA100 0 5 000 µg per plate; assays were carried out in Concentration range: – the presence or absence of rat liver S9 fraction similar to OECD TG 471 (Organisation for Test method: Economic Co-operation and Development, M32F-Y was not mutagenic in either of the assays Result: in the bacterial strains tested either with or without metabolic activation provided by rat liver S9 fraction Reverse Mutation Assay on M32F-Y (Thompson, Salmonella typhimurium 1996b) TA1535, TA1537, TA1538, TA98 Strains: S. typhimurium and TA100 0 5 000 µg per plate; assays were carried out in Concentration range: – the presence or absence of rat liver S9 fraction similar to OECD TG 471 (Organisation for Test method: Economic Co-operation and Development, M32F-M was not mutagenic in either of the assays Result: in the bacterial strains tested either with or without
concentration 24 hours* 48 hours* 24 48 25% 0/20** 0/20 0/10 0/10 50% 0/20 0/20 0/10 0/10 * time after patch removal ** number of animals exhibiting positive response Test method: Buehler delayed contact hypersensitivity study based on OECD TG 406 (Organisation for Economic Co-operation and Development, Result: M32F was not a skin sensitiser when tested in guinea pigs Salmonella typhimurium Reverse Mutation Assay on M32F-Y (Thompson, 1996a) Strains: S. typhimurium TA1535, TA1537, TA1538, TA98 and TA100 Concentration range: 0 – 5 000 µg per plate; assays were carried out in the presence or absence of rat liver S9 fraction Test method: similar to OECD TG 471 (Organisation for Economic Co-operation and Development, Result: M32F-Y was not mutagenic in either of the assays in the bacterial strains tested either with or without metabolic activation provided by rat liver S9 fraction Salmonella typhimurium Reverse Mutation Assay on M32F-Y (Thompson, 1996b) Strains: S. typhimurium TA1535, TA1537, TA1538, TA98 and TA100 Concentration range: 0 – 5 000 µg per plate; assays were carried out in the presence or absence of rat liver S9 fraction Test method: similar to OECD TG 471 (Organisation for Economic Co-operation and Development, Result: M32F-M was not mutagenic in either of the assays in the bacterial strains tested either with or without
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