presence of metabolic activation, in the duplicate experiment the limit was nearly exceeded under same conditions; weakly mutagenic with metabolic activation 9.3.2 In Vitro cytogenetic assay in Chinese hamster V79 cells (16) Dose levels: Experiment 1 18h: 3, 10, 30 g/ml 28h: 100g/ml (without metabolic activation) 18h: 30, 50, 100 g/ml (with metabolic activation) Experiment 2 18h: 100, 300, 500g/ml 28h: 500g/ml (without metabolic activation 18h: 50, 100, 300 g/ml 28h: 500 g/ml Experiment 3 18h: 100, 500, 500g/ml 28h: 500g/ml (without metabolic activation 18h: 100, 300, 500 g/ml 28h: 400 g/ml Metabolic activation: rat liver S9 Test Method: 84/449/EEC Test B10 (27) Toxicologically significant observations: in experiment 3, in the absence of metabolic activation, toxicity was observed with 1000 g/ml (28 h interval; 27.8%) and 800 g/ml (28 h interval; 53.3%); in experiment 3 in the presence of metabolic activation toxicity was observed with 500 g/ml (18 h interval; 16.1%) at other dose levels there were no significant reduction in mitotic index to infer toxicity Result: the notified chemical showed clastogenic effects in Chinese hamster V79 cells in vitro with metabolic activation 9.4 Overall Assessment of Toxicological Data
presence of metabolic activation, in the duplicate experiment the limit was nearly exceeded under same conditions; weakly mutagenic with metabolic activation 9.3.2 In Vitro cytogenetic assay in Chinese hamster V79 cells (16) Dose levels: Experiment 1 18h: 3, 10, 30 g/ml 28h: 100g/ml (without metabolic activation) 18h: 30, 50, 100 g/ml (with metabolic activation) Experiment 2 18h: 100, 300, 500g/ml 28h: 500g/ml (without metabolic activation 18h: 50, 100, 300 g/ml 28h: 500 g/ml Experiment 3 18h: 100, 500, 500g/ml 28h: 500g/ml (without metabolic activation 18h: 100, 300, 500 g/ml 28h: 400 g/ml Metabolic activation: rat liver S9 Test Method: 84/449/EEC Test B10 (27) Toxicologically significant observations: in experiment 3, in the absence of metabolic activation, toxicity was observed with 1000 g/ml (28 h interval; 27.8%) and 800 g/ml (28 h interval; 53.3%); in experiment 3 in the presence of metabolic activation toxicity was observed with 500 g/ml (18 h interval; 16.1%) at other dose levels there were no significant reduction in mitotic index to infer toxicity Result: the notified chemical showed clastogenic effects in Chinese hamster V79 cells in vitro with metabolic activation 9.4 Overall Assessment of Toxicological Data
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