Microbiology Lab (BIOL 2230) SPRING 2009 PREP SHEETS LABS 1 THROUGH 3 – – WK Topics Orgs Media Media/Equipment 3 (3-6) Per Table: Gram Stain Per Table: 18 to 24 hour cultures Per Students: 1 bottle immersion oil Students will Gram stain one 9/11 none 2 packets lens paper broth” smear and one gum “mixed NA 1 dropper bottle each: line smear. For Class Cultures deionized water E. coli [2 NAS] x 6 tables crystal violet S. aureus [2 NB] x 6 tables Gram’s iodine NA broth: 95% EtOH safranin E. coli 2 water spray bottles S. aureus 1 package bibulous paper *Each student will take one LOOP 2 Bunsen burners of each broth culture and mix 1 striker directly onto slide to be stained. 1 Lysol spray bottle Per Student: 1 microscope 1 box of slides 1 clothespin (slide holder) 1 staining tray 1 inoculating loop On Side Bench: beaker w/10% bleach (slide disposal) autoclave tray (culture disposal) slide warmer
Microbiology Lab (BIOL 2230) – SPRING 2009 PREP SHEETS – LABS 1 THROUGH 3 WK Topics Orgs Media Media/Equipment 3 9/11 Gram Stain (3-6) Students will Gram stain one “mixed broth” smear and one gum line smear. Per Table: 18 to 24 hour cultures NA E. coli S. aureus NA broth: E. coli S. aureus *Each student will take one LOOP of each broth culture and mix directly onto slide to be stained. Per Students: none For Class Cultures [2 NAS] x 6 tables [2 NB] x 6 tables Per Table: 1 bottle immersion oil 2 packets lens paper 1 dropper bottle each: deionized water crystal violet Gram’s iodine 95% EtOH safranin 2 water spray bottles 1 package bibulous paper 2 Bunsen burners 1 striker 1 Lysol spray bottle Per Student: 1 microscope 1 box of slides 1 clothespin (slide holder) 1 staining tray 1 inoculating loop On Side Bench: beaker w/10% bleach (slide disposal) autoclave tray (culture disposal) slide warmer
Microbiology%202230%20Prep%20-%201-3.docx