SEPARATION TECHNIQUES OPTIONAL ELECTIVE –III Objectives: The course is designed to import knowledge in the field of various separation techniques in the context of their applications both at laboratory and industry level. The techniques such as GC, HPLC, Electrophoresis etc. Methods allow qualitative and quantitative estimations and thus demand for the development and validation of methods. UNIT: I Column Chromatography and Short column chromatography: Column packing, sample loading, column development, detection. chromatography and Vacuum liquid chromatography: Objectives, optimization studies, selecting column and stationary phases, selecting suitable mobile phases, automated flash chromatography, and reverse phase flash chromatography. UNIT-II Analysis of drugs from formulations and biological samples including, Sample Preparation - selection of biological sample, extraction of drugs by various methods such as Liquid Liquid Extraction (LLE), Solid Phase Extraction (SPE) and Membrane filtration. UNIT: III HPLC: Principles, basic parameters Retention factor, Capacity factor, Selectivity factor, plate number, plate height, resolution, peak shapes, band broadening, van Deemter equation and curve. Column selection and optimization, column problems, solvents, trouble shooting, sample preparation. Method Development and validation: Introduction, Forced Degradation Studies Experimental Approach to Forced Degradation Studies. Stability Indicating HPLC Method Development - Method Scope, Preliminary Requirements, Method Development Approach, Method Optimization and validation. UNIT-IV Gas Chromatography: Principles, injector, head space sampling, columns for GC, detectors, quantification, derivatization techniques. Hyphenated techniques: Introduction to GC-MS and LC-MS techniques and their applications. UNIT-V Electrophoresis: Capillary electrophoresis: principle (zeta potential), instrumentation, different modes of CE, advantages and disadvantages, pharmaceutical applications. Counter current chromatography: principles, droplet counter current chromatography, centrifugal partition chromatography, choice of solvents for SP and MP.
OPTIONAL ELECTIVE –III SEPARATION TECHNIQUES Objectives: The course is designed to import knowledge in the field of various separation techniques in the context of their applications both at laboratory and industry level. The techniques such as GC, HPLC, Electrophoresis etc. Methods allow qualitative and quantitative estimations and thus demand for the development and validation of methods. UNIT: I Column Chromatography and Short column chromatography: Column packing, sample loading, column development, detection. chromatography and Vacuum liquid chromatography: Objectives, optimization studies, selecting column and stationary phases, selecting suitable mobile phases, automated flash chromatography, and reverse phase flash chromatography. UNIT-II Sample Preparation - Analysis of drugs from formulations and biological samples including, selection of biological sample, extraction of drugs by various methods such as Liquid Liquid Extraction (LLE), Solid Phase Extraction (SPE) and Membrane filtration. UNIT: III HPLC: Principles, basic parameters Retention factor, Capacity factor, Selectivity factor, plate number, plate height, resolution, peak shapes, band broadening, van Deemter equation and curve. Column selection and optimization, column problems, solvents, trouble shooting, sample preparation. Method Development and validation: Introduction, Forced Degradation Studies Approach to Forced Degradation Studies. Stability Indicating HPLC Method Development - Method Scope, Preliminary Requirements, Method Development Approach, Method Optimization and validation. UNIT-IV Gas Chromatography: Principles, injector, head space sampling, columns for GC, detectors, quantification, derivatization techniques. Hyphenated techniques: Introduction to GC-MS and LC-MS techniques and their applications. UNIT-V Electrophoresis: Capillary electrophoresis: principle (zeta potential), instrumentation, different modes of CE, advantages and disadvantages, pharmaceutical applications. Counter current chromatography: principles, droplet counter current chromatography, centrifugal partition chromatography, choice of solvents for SP and MP. Experimental
MPharm_PHARMACEUTICS_AND_PHARMACEUTICAL_TECHONOLOGY.docx