1 mL of test substance at 20, 140 and 1 000 Dose/Study mg/kg/day in 0.5% Tween 80 in 0.5% CMC- sodium aqueous solution for 28 days 140 mg/kg/day Low dose: 1 000 mg/kg/day Mid dose: High 1 mL of 0.5% 80 in 0.5% CMC-sodium dose: aqueous solution Control: 1 mL of test substance at 1 000mg/kg/day in 0.5% Recovery Tween 80 in 0.5% CMC-sodium aqueous solution group (test): 1 mL of 0.5% Tween 80 in 0.5% CMC-sodium Recovery aqueous solution group (control): recovery group animals were kept for 14 day recovery period following termination of treatment 20 mg/kg/day The Japanese Guidelines applied to Industrial Test method: Chemicals in (JGAIC, 1986) nil Mortality: Clinical observations: Test group: all animals in all groups showed increased in body weights, food consumption and consumption similar to the control and recovery groups mid dose: salivation was observed in one male on and after 22 days of treatment and in one female on and after 24 days of treatment; salivation disappeared within 40 minutes salivation in both on and after 5 days of high dose: treatment
Dose/Study 1 mL of test substance at 20, 140 and 1 000 mg/kg/day in 0.5% Tween 80 in 0.5% CMC- sodium aqueous solution for 28 days Low dose: Mid dose: High dose: Control: Recovery group (test): Recovery group (control): 20 mg/kg/day 140 mg/kg/day 1 000 mg/kg/day 1 mL of 0.5% 80 in 0.5% CMC-sodium aqueous solution 1 mL of test substance at 1 000mg/kg/day in 0.5% Tween 80 in 0.5% CMC-sodium aqueous solution 1 mL of 0.5% Tween 80 in 0.5% CMC-sodium aqueous solution recovery group animals were kept for 14 day recovery period following termination of treatment Test method: The Japanese Guidelines applied to Industrial Chemicals in (JGAIC, 1986) Mortality: nil Clinical observations: Test group: mid dose: high dose: all animals in all groups showed increased in body weights, food consumption and consumption similar to the control and recovery groups salivation was observed in one male on and after 22 days of treatment and in one female on and after 24 days of treatment; salivation disappeared within 40 minutes salivation in both on and after 5 days of treatment
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