level of alkaline phosphatase in males at 400 mg.kg-1.d-1; statistically significant changes in liver weight and haematological parameters were observed at 1 000 mg.kg-1.d-1 9.3 Genotoxicity 9.3.1 and Reverse Mutation Assay Salmonella typhimurium Escherichia coli (Nishiuchi, 1991) TA1535, TA1537, TA98 and Strains: S. typhimurium TA100; WP2uvrA Escherichia coli 0 - 5000 µg per plate; assays were carried out in Concentration the presence or absence of rat liver S9 fraction similar to OECD TG 471 (Organisation for Test method: Economic Co-operation and Development, the notified chemical was not mutagenic in either Result: of the assays in the bacterial strains tested either with or without metabolic activation provided by rat S9 fraction 9.3.2 and Reverse Mutation Assay Salmonella typhimurium Escherichia coli (Thompson, 1993) TA1535, TA1537, TA98 and Strains: S. typhimurium TA100; WP2uvrA Escherichia coli 0 - 5000 µg per plate; assays were carried out in Concentration range: the presence or absence of rat liver S9 fraction similar to OECD TG 471 (Organisation for Test method: Economic Co-operation and Development, the notified chemical was not mutagenic in either Result: of the assays in the bacterial strains tested either with or without metabolic activation provided by rat liver S9 fraction 9.3.3 Chromosomal Aberration Assay in Chinese Hamster Lung Cells (Wright, 1993)
level of alkaline phosphatase in males at 400 mg.kg-1.d-1; statistically significant changes in liver weight and haematological parameters were observed at 1 000 mg.kg-1.d-1 9.3 Genotoxicity 9.3.1 Salmonella typhimurium and Escherichia coli Reverse Mutation Assay (Nishiuchi, 1991) Strains: S. typhimurium TA1535, TA1537, TA98 and TA100; Escherichia coli WP2uvrA Concentration 0 - 5000 µg per plate; assays were carried out in the presence or absence of rat liver S9 fraction Test method: similar to OECD TG 471 (Organisation for Economic Co-operation and Development, Result: the notified chemical was not mutagenic in either of the assays in the bacterial strains tested either with or without metabolic activation provided by rat S9 fraction 9.3.2 Salmonella typhimurium and Escherichia coli Reverse Mutation Assay (Thompson, 1993) Strains: S. typhimurium TA1535, TA1537, TA98 and TA100; Escherichia coli WP2uvrA Concentration range: 0 - 5000 µg per plate; assays were carried out in the presence or absence of rat liver S9 fraction Test method: similar to OECD TG 471 (Organisation for Economic Co-operation and Development, Result: the notified chemical was not mutagenic in either of the assays in the bacterial strains tested either with or without metabolic activation provided by rat liver S9 fraction 9.3.3 Chromosomal Aberration Assay in Chinese Hamster Lung Cells (Wright, 1993)
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