2. Microstructure parameters of the wet, crosslinked, electrospun scaffolds: average fibre diameter df,wet (±0.08m), pore size dp,wet(±16%) and porosity calculated from measurements from 25 images from 5 samples, estimated specific pore surface area (SSA), water contact angle goniometry (CAG) (from 5 dynamic CAG tests of different samples, mechanical tensile properties in the fibre direction calculated from 6 tests of ), different samples, and also cell seeding efficiency for cells seeded on scaffolds electrospun at 20 kV. Scaffold Pure Gelatin 10 %wt HA - 20 %wt HA - 25 %wt HA - Gelatin composition Gelatin Gelatin Voltage (kV) 20 25 30 20 25 30 20 25 30 20 25 30 for df,wet(m) 5.2 4 3.4 1.8 1.6 1.4 1.5 1.1 1.0 1.2 1 0.7 dp,wet(m) 31.7 25.4 24.5 9.5 8.8 8.2 8.8 8.2 7.8 7.8 7.5 7.2 0.59 0.59 0.55 0.61 0.55 0.52 0.60 0.52 0.50 0.49 0.45 0.41 wet SSA (m2/g) 0.74 0.94 1.09 2.00 2.23 2.53 2.31 3.10 3.36 2.83 3.35 4.67 30 20 22 70 31 25 40 27 29 20 18 16 o (o) 12 20 12 28 10 10 27 26 28 5 5 4 (o) t 17 2 1 2 1 0.6 1 1 1 0.7 0.7 0.5 tspread (s) modulus 255 278 282 280 286 404 327 400 452 490 625 925 ±70 ±50 ±60 ±70 ±120 ±100 ±30 ±110 ±110 ±120 ±200 ±100 (MPa)
2. Microstructure parameters of the wet, crosslinked, electrospun scaffolds: average fibre diameter df,wet (±0.08m), pore size dp,wet(±16%) and porosity calculated from measurements from 25 images from 5 samples, estimated specific pore surface area (SSA), water contact angle goniometry (CAG) (from 5 dynamic CAG tests of different samples, ), mechanical tensile properties in the fibre direction calculated from 6 tests of different samples, and also cell seeding efficiency for cells seeded on scaffolds electrospun at 20 kV. Scaffold composition Pure Gelatin 10 %wt HA - Gelatin 20 %wt HA - Gelatin 25 %wt HA - Gelatin Voltage (kV) for 20 25 30 20 25 30 20 25 30 20 25 30 df,wet(m) 5.2 4 3.4 1.8 1.6 1.4 1.5 1.1 1.0 1.2 1 0.7 dp,wet(m) 31.7 25.4 24.5 9.5 8.8 8.2 8.8 8.2 7.8 7.8 7.5 7.2 wet 0.59 0.59 0.55 0.61 0.55 0.52 0.60 0.52 0.50 0.49 0.45 0.41 SSA (m2/g) 0.74 0.94 1.09 2.00 2.23 2.53 2.31 3.10 3.36 2.83 3.35 4.67 o (o) t (o) tspread (s) 30 12 17 20 20 2 22 12 1 70 28 2 31 10 1 25 10 0.6 40 27 1 27 26 1 29 28 1 20 5 0.7 18 5 0.7 16 4 0.5 modulus (MPa) 255 ±70 278 ±50 282 ±60 280 ±70 286 ±120 404 ±100 327 ±30 400 ±110 452 ±110 490 ±120 625 ±200 925 ±100
OneLayerBoneTissueEng-JBMR-A-Accepted.docx