each rabbit received 0.1 mL undiluted material Method of administration: placed into the everted lower lid of the right eye, with the left eye serving as the untreated control Draize scores (Draize, 1959) of eyes: Time after instillation Animal 1 day 2 days 3 days 4 days 7 days 14 days 21 days see Attachment 1 for Draize scales 1 opacity area redness chemosis discharge o a r c d Sodium sodium fluorescein examination results showed fluorescein 1 rabbit became negative after 48 hours and examination: other 2 became negative after day 7 OECD TG 405 (Organisation for Economic Test method: Co- operation and Development, 1995-1996) The test material caused broad corneal opacity, Comments: lesions of the iris and moderate to severe irritation of the conjunctivae. Irritation was still present in two animals at the end of the observation period (21 days) the test material (44% notified chemical) Result: caused severe damage to the eyes of rabbits
Method of administration: each rabbit received 0.1 mL undiluted material placed into the everted lower lid of the right eye, with the left eye serving as the untreated control Draize scores (Draize, 1959) of eyes: Time after instillation Animal 1 day 2 days 3 days 4 days 7 days 14 days 21 days 1 see Attachment 1 for Draize scales o opacity a area r redness c chemosis d discharge Sodium fluorescein examination: sodium fluorescein examination results showed 1 rabbit became negative after 48 hours and other 2 became negative after day 7 Test method: OECD TG 405 (Organisation for Economic Co- operation and Development, 1995-1996) Comments: The test material caused broad corneal opacity, lesions of the iris and moderate to severe irritation of the conjunctivae. Irritation was still present in two animals at the end of the observation period (21 days) Result: the test material (44% notified chemical) caused severe damage to the eyes of rabbits
NA625FR.docx