Biological Safety Cabinets used for okay to store pipette tips and It’s infectious / potentially infectious material microcentrifuge tubes in the biological must be certified at least: safety cabinet as long as they are pushed up against the back wall of the Daily cabinet. Monthly True Yearly False Once Which of the following practices should Which of the following protects both the be utilized when working in a biological user and the material that is being safety cabinet? handled: Disinfect all surfaces of the BSC Fume hood before and after work Clean bench Disinfect all items which go into and Biological Safety Cabinet (BSC) come out of the BSC All of the above Allow the BSC to operate before work begins and after work ceases 30. In a Class II, Type A2 biological safety All of the above cabinet (typical unit found in most labs): Downward laminar flow splits 2-6 If a spill should occur within a Biological inches above work surface between Safety Cabinet (BSC), what steps front and rear grills would immediately be taken? HEPA-filtered air from the cabinet is A. Turn off the BSC, grab the biological re-circulated in the unit and also spill kit, and immediately wipe up the exhausts to the room spill with a dry, absorbent towel It is safe to work with substantial Turn off the BSC and pour a amounts of chemicals and germicidal agent on the spill radiolabeled nucleotides A and B only Turn off the BSC, evacuate the laboratory, and notify company safety authorities 31. Open flames are not allowed in a biological safety cabinet because Leave the BSC on, grab the biological spill kit, add absorbent They disrupt airflow patterns that material and germicidal agent, wipe protect user and product up the spill once contact time has They can damage HEPA filters permitted inactivation, and remove They pose a fire hazard corrosive residue with 70% ethanol. All of the above Leave the BSC on, grab the biological spill kit and wipe up the Acceptable alternatives to Bunsen spill with a dry, absorbent towel burner use in the biological safety cabinet (BSC) include: 36. Disinfectants decontaminate soiled Disposable loops, spreaders, or and clean surfaces equally well. glass beads True Bacticinerator False Wrist-operated Touch-O-Matic burner All of the above
Biological Safety Cabinets used for infectious / potentially infectious material must be certified at least: Daily Monthly Yearly Once Which of the following protects both the user and the material that is being handled: Fume hood Clean bench Biological Safety Cabinet (BSC) All of the above 30. In a Class II, Type A2 biological safety cabinet (typical unit found in most labs): Downward laminar flow splits 2-6 inches above work surface between front and rear grills HEPA-filtered air from the cabinet is re-circulated in the unit and also exhausts to the room It is safe to work with substantial amounts of chemicals and radiolabeled nucleotides A and B only 31. Open flames are not allowed in a biological safety cabinet because They disrupt airflow patterns that protect user and product They can damage HEPA filters They pose a fire hazard All of the above Acceptable alternatives to Bunsen burner use in the biological safety cabinet (BSC) include: Disposable loops, spreaders, or glass beads Bacticinerator Wrist-operated Touch-O-Matic burner All of the above It’s okay to store pipette tips and microcentrifuge tubes in the biological safety cabinet as long as they are pushed up against the back wall of the cabinet. True False Which of the following practices should be utilized when working in a biological safety cabinet? Disinfect all surfaces of the BSC before and after work Disinfect all items which go into and come out of the BSC Allow the BSC to operate before work begins and after work ceases All of the above If a spill should occur within a Biological Safety Cabinet (BSC), what steps would immediately be taken? A. Turn off the BSC, grab the biological spill kit, and immediately wipe up the spill with a dry, absorbent towel Turn off the BSC and pour a germicidal agent on the spill Turn off the BSC, evacuate the laboratory, and notify company safety authorities Leave the BSC on, grab the biological spill kit, add absorbent material and germicidal agent, wipe up the spill once contact time has permitted inactivation, and remove corrosive residue with 70% ethanol. Leave the BSC on, grab the biological spill kit and wipe up the spill with a dry, absorbent towel 36. Disinfectants decontaminate soiled and clean surfaces equally well. True False