Figure S3. Effects of BAG3 on MTOR and AMPK phosphorylation. (A) HeLa cells were transfected with (siControl) or (siBAG3) siRNAs. (B) HeLa cells were control BAG3 transfected with (pControl) or (pBAG3) plasmids. Protein levels were control BAG3 analyzed by western blot. Representative images and quantification of pAMPK normalized by total AMPK and pMTOR normalized by total MTOR are shown. Data are expressed as mean ± SEM of at least 3 independent experiments. Statistical significance was calculated with Student t-test. There were nonsignificant differences.
Figure S3. Effects of BAG3 on MTOR and AMPK phosphorylation. (A) HeLa cells were transfected with control (siControl) or BAG3 (siBAG3) siRNAs. (B) HeLa cells were transfected with control (pControl) or BAG3 (pBAG3) plasmids. Protein levels were analyzed by western blot. Representative images and quantification of pAMPK normalized by total AMPK and pMTOR normalized by total MTOR are shown. Data are expressed as mean ± SEM of at least 3 independent experiments. Statistical significance was calculated with Student t-test. There were nonsignificant differences.