Figure S2. Effect of BAG3 on the levels of ATG proteins. HeLa cells were transfected with (siControl) or (siBAG3) siRNAs. Protein levels were analyzed by western control BAG3 blot and quantification of ATG3 (A), ATG4B (C), ATG12–ATG5 (E), ATG7 (G) normalized by ACTB are shown. Also, HeLa cells were transfected with (pControl) control or (pBAG3) plasmids. Protein levels were analyzed by western blot and BAG3 quantification of ATG3 (B), ATG4B (D), ATG12–ATG5 (F), ATG7 (H) normalized by ACTB are shown. Data are expressed as mean ± SEM of at least 3 independent experiments. Statistical significance was calculated using Student t-test. There were nonsignificant differences.
Figure S2. Effect of BAG3 on the levels of ATG proteins. HeLa cells were transfected with control (siControl) or BAG3 (siBAG3) siRNAs. Protein levels were analyzed by western blot and quantification of ATG3 (A), ATG4B (C), ATG12–ATG5 (E), ATG7 (G) normalized by ACTB are shown. Also, HeLa cells were transfected with control (pControl) or BAG3 (pBAG3) plasmids. Protein levels were analyzed by western blot and quantification of ATG3 (B), ATG4B (D), ATG12–ATG5 (F), ATG7 (H) normalized by ACTB are shown. Data are expressed as mean ± SEM of at least 3 independent experiments. Statistical significance was calculated using Student t-test. There were nonsignificant differences.