STUDENT ACTIVITY BENEDICT’S SOLUTION Materials Hot plate Sharpie 500 mL beaker Metric ruler Test tube rack and test tube Dropper holder reagent Benedict’s 10 test tube Test solution: distilled water, clear diet soda, clear non-diet soda, pineapple juice, onion juice, potato juice, milk, glucose solution, sucrose solution, corn syrup, and tap water. [Warning: reagent is corrosive. If it splashes onto your skin, wash the area Benedict’s immediately with soap and water.] Note: Label each transfer pipette with the name of the solutions that are distributed to you. Procedure 5.5 Reagent test Benedict’s [Warning: Benedict reagent is the only solution you will heat up.] Using a sharpie, label test tube 1-15 Pipette 2 mL of the correct stock solution into the test tubes, as described in Table 5.4. Add 2 mL of solution to each test tube agitate the mixture by shaking the test Benedict’s tubes from side to side or with a vortex mixer, and record the color of the mixture in Table 5.4. Remove the test tube with a test Heat the test tubes in boiling water for 3 minutes. tube holder, and record the color of each test tube in Table 5.4. Discard all the solution according to the each test tube and instructor’s directions. Wash return them to your station. Table 5.4 Reagent Test for Reducing Sugars Benedict’s Test What color is it What color is it Tube Solution Before heating? after heating? Conclusion Distilled water 1 Glucose solution 2 Maltose solution 3 4 Lactose solution
STUDENT ACTIVITY BENEDICT’S SOLUTION Materials Hot plate 500 mL beaker Test tube rack and test tube holder 10 test tube Sharpie Metric ruler Dropper Benedict’s reagent Test solution: distilled water, clear diet soda, clear non-diet soda, pineapple juice, onion juice, potato juice, milk, glucose solution, sucrose solution, corn syrup, and tap water. [Warning: Benedict’s reagent is corrosive. If it splashes onto your skin, wash the area immediately with soap and water.] Note: Label each transfer pipette with the name of the solutions that are distributed to you. Procedure 5.5 Benedict’s Reagent test [Warning: Benedict reagent is the only solution you will heat up.] Using a sharpie, label test tube 1-15 Pipette 2 mL of the correct stock solution into the test tubes, as described in Table 5.4. Add 2 mL of Benedict’s solution to each test tube agitate the mixture by shaking the test tubes from side to side or with a vortex mixer, and record the color of the mixture in Table 5.4. Heat the test tubes in boiling water for 3 minutes. Remove the test tube with a test tube holder, and record the color of each test tube in Table 5.4. Discard all the solution according to the instructor’s directions. Wash each test tube and return them to your station. Table 5.4 Benedict’s Reagent Test for Reducing Sugars Test Tube Solution What color is it Before heating? What color is it after heating? Conclusion 1 Distilled water 2 Glucose solution 3 Maltose solution 4 Lactose solution