RESULTS Concentration mg/L Number of Fish Mortality Nominal Actual 1 h 24 h 48 h 72 h 96 h 0 0.9 (DOC) 7 0 0 0 0 0 100 31 (DOC) 7 0 0 0 0 0 LC50 > Water solubility at 96 hours. NOEC Water solubility at 96 hours. Remarks Results All fish exhibited normal behaviour – CONCLUSION The analogue polymer showed no toxicity to fish at the limit of water solubility. TEST FACILITY Noack Laboratorium (2002b) Acute toxicity to aquatic invertebrates TEST SUBSTANCE Notified polymer METHOD OECD TG 202 Daphnia sp. Acute Immobilisation Test and Reproduction Test - static. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 271 mg CaCO3/L Analytical Monitoring DOC Remarks - Method The mean DOC level was 55.9 mg/L at test initiation and 61.6 mg/L at termination. RESULTS Concentration mg/L Number of D. magna Number Immobilised Nominal Actual 24 h 48 h 0 20 0 0 100 58.8 (DOC) 20 0 0 LC50 > 100 mg/L at 48 hours NOEC 100 mg/L at 48 hours Remarks - Results The response (EC50 = 1.66 mg/L) to the reference substance potassium dichromate was within the prescribed range (0.6-2.1 mg/L). CONCLUSION The notified polymer is not harmful to daphnids TEST FACILITY Noack Laboratorien (2009a) Algal growth inhibition test TEST SUBSTANCE Notified polymer METHOD OECD TG 201 Alga, Growth Inhibition Test. Species Desmodesmus subspicatus Exposure Period 72 hours Concentration Range Nominal: 100 mg/L Auxiliary Solvent None Water Hardness Typical algal culture medium (soft water)
RESULTS Concentration mg/L Number of Fish Mortality Nominal Actual 1 h 24 h 48 h 72 h 96 h 0 0.9 (DOC) 7 0 0 0 0 0 100 31 (DOC) 7 0 0 0 0 0 LC50 > Water solubility at 96 hours. NOEC Water solubility at 96 hours. Remarks – Results All fish exhibited normal behaviour CONCLUSION The analogue polymer showed no toxicity to fish at the limit of water solubility. TEST FACILITY Noack Laboratorium (2002b) Acute toxicity to aquatic invertebrates TEST SUBSTANCE Notified polymer METHOD OECD TG 202 Daphnia sp. Acute Immobilisation Test and Reproduction Test - static. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 271 mg CaCO3/L Analytical Monitoring DOC Remarks - Method The mean DOC level was 55.9 mg/L at test initiation and 61.6 mg/L at termination. RESULTS Concentration mg/L Number of D. magna Number Immobilised Nominal Actual 24 h 48 h 0 20 0 0 100 58.8 (DOC) 20 0 0 LC50 > 100 mg/L at 48 hours NOEC 100 mg/L at 48 hours Remarks - Results The response (EC50 = 1.66 mg/L) to the reference substance potassium dichromate was within the prescribed range (0.6-2.1 mg/L). CONCLUSION The notified polymer is not harmful to daphnids TEST FACILITY Noack Laboratorien (2009a) Algal growth inhibition test TEST SUBSTANCE Notified polymer METHOD OECD TG 201 Alga, Growth Inhibition Test. Species Desmodesmus subspicatus Exposure Period 72 hours Concentration Range Nominal: 100 mg/L Auxiliary Solvent None Water Hardness Typical algal culture medium (soft water)