Test Semi Static. – Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 150 mg CaCO3/L Analytical Monitoring Gas chromatography Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles. Concentration mg/L Number of D. magna Immobilised (%) Nominal Actual 24 h [acute] 48 h [acute] Control ND 20 0 0 6.5 ND 20 0 0 13 12 20 0 0 25.0 23 20 0 10 50.0 47 20 100 100 100.0 99 20 100 100 LC50 31 mg/L (95% CI 27-37 mg/L) at 48 h NOEC 12 mg/L at 48 h Remarks - Results All validity criteria of the test guideline were satisfied. The test solutions were renewed daily during the 48 h test period. The 48 h EC50 and NOEC for Daphnia were determined to be 31 mg/L (95% CI 27–37 mg/L) and 12 mg/L, respectively, based on mean measured concentrations. The test substance is considered to be harmful to aquatic invertebrates. Harlan (2012c) Algal growth inhibition test Mixture of notified chemicals OECD TG 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test Static. – Species (green alga) Pseudokirchneriella subcapitata Exposure Period 96 hours Concentration Nominal: 2.2–35 mg/L Actual: 0.99–34 mg/L Auxiliary Solvent None Water Hardness 15 mg CaCO3/L Analytical Monitoring Gas chromatography Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles. Biomass Growth EC50 NOEC EC50 NOEC mg/L at 96 h mg/L mg/L at 96 h mg/L 11 (95% CI 10-13) 2.5 21 (95% CI 19-25) 2.5 Remarks - Results All validity criteria of the test guideline were satisfied. The actual concentrations of the test item were measured at the start of the test period. Over the 96 hours test period, the test item concentrations in the test media decreased. Thus, the mean measured time-weighted exposure concentrations were calculated taking into account the
Test – Semi Static. Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 150 mg CaCO3/L Analytical Monitoring Gas chromatography Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles. Concentration mg/L Number of D. magna Immobilised (%) Nominal Actual 24 h [acute] 48 h [acute] Control ND 20 0 0 6.5 ND 20 0 0 13 12 20 0 0 25.0 23 20 0 10 50.0 47 20 100 100 100.0 99 20 100 100 LC50 31 mg/L (95% CI 27-37 mg/L) at 48 h NOEC 12 mg/L at 48 h Remarks - Results All validity criteria of the test guideline were satisfied. The test solutions were renewed daily during the 48 h test period. The 48 h EC50 and NOEC for Daphnia were determined to be 31 mg/L (95% CI 27–37 mg/L) and 12 mg/L, respectively, based on mean measured concentrations. The test substance is considered to be harmful to aquatic invertebrates. Harlan (2012c) Algal growth inhibition test Mixture of notified chemicals OECD TG 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test – Static. Species Pseudokirchneriella subcapitata (green alga) Exposure Period 96 hours Concentration Nominal: 2.2–35 mg/L Actual: 0.99–34 mg/L Auxiliary Solvent None Water Hardness 15 mg CaCO3/L Analytical Monitoring Gas chromatography Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles. Biomass Growth EC50 NOEC EC50 NOEC mg/L at 96 h mg/L mg/L at 96 h mg/L 11 (95% CI 10-13) 2.5 21 (95% CI 19-25) 2.5 Remarks - Results All validity criteria of the test guideline were satisfied. The actual concentrations of the test item were measured at the start of the test period. Over the 96 hours test period, the test item concentrations in the test media decreased. Thus, the mean measured time-weighted exposure concentrations were calculated taking into account the