After the bath, the extract will be evaporated at 30 degrees Celsius until there is about 10 ml of water. After the root exudates have been carefully collected, they will be placed into cuvettes which will be placed into a Spectrometer. The exudates will be tested at both 405 nm and 500 nm to observe fluorescence with multiple samples collected from each trays (Fig 10). RESULTS After experimentation and observing the fluorescence of each solution we focused on the peaks of each of the wavelengths. The peaks indicated which solution of the three tested showed the most fluorescence. The more the fluorescence the less the quenching and the goal was to observe more or similar quenching by the Iron Solution as that emitted by Copper Solution. Looking at both graphs for 405 nm, it is seen that the Control which contained no solution emitted the most florescence as expected at .14 RFU (Relative Fluorescence Unit) and then below that came the fluorescence from the Iron Solution at .10 RFU and finally came the Copper which quenched the most at .08 RFU (Fig. 11 and 12). The same test was run again with a different sample of exudates from all three solution but this time they were tested on 500nm on the SpectroVis. Just like the 405 nm the results were surprisingly similar with the control test giving out the highest fluorescence of .75 RFU which is significantly higher than that observed at 405 nm (Fig. 13). The Iron again came I second with a peak of .72 RFU a little lower than the control and then the Copper, as expected quenched the most at .5 RFU (Fig. 14 and 15). The pattern continued as more samples were
After the bath, the extract will be evaporated at 30 degrees Celsius until there is about 10 ml of water. After the root exudates have been carefully collected, they will be placed into cuvettes which will be placed into a Spectrometer. The exudates will be tested at both 405 nm and 500 nm to observe fluorescence with multiple samples collected from each trays (Fig 10). RESULTS After experimentation and observing the fluorescence of each solution we focused on the peaks of each of the wavelengths. The peaks indicated which solution of the three tested showed the most fluorescence. The more the fluorescence the less the quenching and the goal was to observe more or similar quenching by the Iron Solution as that emitted by Copper Solution. Looking at both graphs for 405 nm, it is seen that the Control which contained no solution emitted the most florescence as expected at .14 RFU (Relative Fluorescence Unit) and then below that came the fluorescence from the Iron Solution at .10 RFU and finally came the Copper which quenched the most at .08 RFU (Fig. 11 and 12). The same test was run again with a different sample of exudates from all three solution but this time they were tested on 500nm on the SpectroVis. Just like the 405 nm the results were surprisingly similar with the control test giving out the highest fluorescence of .75 RFU which is significantly higher than that observed at 405 nm (Fig. 13). The Iron again came I second with a peak of .72 RFU a little lower than the control and then the Copper, as expected quenched the most at .5 RFU (Fig. 14 and 15). The pattern continued as more samples were