confirm that a number of stabilising cosolutes are preferentially excluded from the surface of BSA, and more specifically that the degree of exclusion corresponds with the cosolute’s ability as an osmoprotectant (Courtenay et al. 2000). The same group also demonstrated preferential interaction of both urea and guanidinium with BSA using the same technique (Courtenay et al. 2001). Table . Preferential interaction data recorded in the literature for a selection of stabilising small molecules and denaturants Cosolute Preferentially Proteins used Experimental Reference bound or technique excluded used Stabilising cosolutes Arginine (1) <0.5 M (1) BSA, Lysozyme, (1)VPO, (1)(Schneider & Neutral, >0.5 M Densimetry Trout 2009) Excluded (2) Lysozyme (2;3;4) High (2&3)(Kita et al. (2) Excluded (3) BSA precision 1994) (3) Neutral (4) RNase A densimetry (4)(Lin & (4) Weakly Timasheff 1996) excluded Betaine Excluded (1) Lysozyme (1;2) High (1)(Arakawa & (2;3) BSA precision Timasheff 1985) densimetry (2)(Arakawa & (3) VPO Timasheff 1983) (3)(Courtenay et al. 2000) Glucose Excluded RNase A, Lysozyme, High precision (Arakawa & Chymotrysinogen A, densimetry Timasheff 1982b) BSA, Ovalbumin Glycerol Excluded (1;2) BSA (1;2) VPO (1)(Courtenay et (2) Lysozyme, (2) High al. 2000)
confirm that a number of stabilising cosolutes are preferentially excluded from the surface of BSA, and more specifically that the degree of exclusion corresponds with the cosolute’s ability as an osmoprotectant (Courtenay et al. 2000). The same group also demonstrated preferential interaction of both urea and guanidinium with BSA using the same technique (Courtenay et al. 2001). Table . Preferential interaction data recorded in the literature for a selection of stabilising small molecules and denaturants Cosolute Preferentially bound or excluded Proteins used Experimental technique used Reference Stabilising cosolutes Arginine (1) <0.5 M Neutral, >0.5 M Excluded (2) Excluded (3) Neutral (4) Weakly excluded (1) BSA, Lysozyme, (2) Lysozyme (3) BSA (4) RNase A (1)VPO, Densimetry (2;3;4) High precision densimetry (1)(Schneider & Trout 2009) (2&3)(Kita et al. 1994) (4)(Lin & Timasheff 1996) Betaine Excluded (1) Lysozyme (2;3) BSA (1;2) High precision densimetry (3) VPO (1)(Arakawa & Timasheff 1985) (2)(Arakawa & Timasheff 1983) (3)(Courtenay et al. 2000) Glucose Excluded RNase A, Lysozyme, Chymotrysinogen A, BSA, Ovalbumin High precision densimetry (Arakawa & Timasheff 1982b) Glycerol Excluded (1;2) BSA (2) Lysozyme, (1;2) VPO (2) High (1)(Courtenay et al. 2000)