Kovac’s reagent when any additives are to be added. Below is an example of the label from a Day One Protocol: bottle of media. 1) obtain all the necessary media and label each plate and tube with your initials Label from a bottle of EMB Figure 1 – agar Nutritional Requirements of Bacteria In this section we will be inoculating several different media with 4 different Materials: bacteria and determining if they will 1 plate plain agar grow on the different media. Each 1 plate M-9 agar with glucose media has a different level of nutrients 1 plate nutrient agar and each organism has a different set of 1 plate blood agar nutritional requirements. To set up this 1 plate MSA section of the lab follow these steps: 1plate EMB agar using your wax pencil divide the 1 DNase agar following plates into 4 equal portions 2 tubes of TSI agar slants and label each quadrant with the 2 tubes of BHI broth initials of one of the organisms to be 2 tubes of urea broth used Pa, Spy, Se or Ec – bacterial cultures: plain agar S. aureus M-9 plus glucose agar S. epidermidis nutrient agar E. coli agar Be sure to write on the bottom of the P. vulgaris plates not the tops using aseptic technique, inoculate S. pyogenes each plate in the appropriate P. aeruginosa quadrant with a small amount of the S. typhimurium culture bacterial culture. The instructor will Gram stain reagents give specific instructions on how to glass microscope slides perform the inoculation. Be sure not wax pencils to spread the organism beyond the Bunsen burner bounds of the quadrant. incubate the plates at 37oC overnight inoculating loop
when any additives are to be added. Below is an example of the label from a bottle of media. Figure 1 – Label from a bottle of EMB agar Materials: 1 plate plain agar 1 plate M-9 agar with glucose 1 plate nutrient agar 1 plate blood agar 1 plate MSA 1plate EMB agar 1 DNase agar 2 tubes of TSI agar slants 2 tubes of BHI broth 2 tubes of urea broth bacterial cultures: S. aureus S. epidermidis E. coli P. vulgaris S. pyogenes P. aeruginosa S. typhimurium culture Gram stain reagents glass microscope slides wax pencils Bunsen burner inoculating loop Kovac’s reagent Day One Protocol: 1) obtain all the necessary media and label each plate and tube with your initials Nutritional Requirements of Bacteria In this section we will be inoculating several different media with 4 different bacteria and determining if they will grow on the different media. Each media has a different level of nutrients and each organism has a different set of nutritional requirements. To set up this section of the lab follow these steps: using your wax pencil divide the following plates into 4 equal portions and label each quadrant with the initials of one of the organisms to be used – Pa, Spy, Se or Ec plain agar M-9 plus glucose agar nutrient agar agar Be sure to write on the bottom of the plates not the tops using aseptic technique, inoculate each plate in the appropriate quadrant with a small amount of the bacterial culture. The instructor will give specific instructions on how to perform the inoculation. Be sure not to spread the organism beyond the bounds of the quadrant. incubate the plates at 37oC overnight