APPENDIX C: ENVIRONMENTAL FATE ECOTOXICOLOGICAL INVESTIGATIONS AND Environmental Fate Ready biodegradability TEST SUBSTANCE Analogue of notified chemical METHOD International Standards Organisation method 7827 “Water Quality- Evaluation in an Aqueous Medium of the Aerobic Biodegradability of Organic Compounds, Method by Dissolved Organic Carbon” (1984) Inoculum Aerobically growing activated sludge from pilot scale sewage treatment plant Exposure Period Not stated Auxiliary Solvent None Analytical Monitoring Dissolved organic carbon determination Remarks - Method The culture medium was an aqueous solution containing several minerals and micronutrients and set to a pH of ~7. The test substance was added to the culture medium to give a DOC of 30 ppm. The medium was inoculated with 7 mL fresh aerobic activated sludge to give a suspended solids content of 30 mg/L. The culture was shaken continuously at 140- 150 rpm for the duration of the test. The temperature was maintained at 27 ± 2ºC which is outside the range specified in the method (20 to 25ºC). The biodegradation test included duplicate cultures of the test substance, a blank culture (containing only test medium and activated sludge inoculum), an inhibition test culture and an abiotic test culture. The inhibition test culture contained the test substance and sodium acetate both at levels of 30 ppm DOC. The abiotic blank culture contained the test substance in an inoculated culture that was sterilised by addition of 4 mL/L hypochlorite solution. Samples for DOC analysis (~35 mL) were withdrawn at the required intervals and centrifuged at 2,275 for 15 min. g RESULTS Test substance Sodium Acetate Day % Degradation* Day % Degradation 2 ~95% 3 >94% 7 ~98% *Information taken from a graph (DOC vs time) Remarks - Results The duration of the test was not stated, however graphical information in the paper indicates the test substance was analysed over 9 days which is adequate considering it has a measured biodegradability half life of <9.84 h. CONCLUSION The test substance and hence the notified chemical is readily biodegradable TEST FACILITY Exempt information
APPENDIX C: ENVIRONMENTAL FATE AND ECOTOXICOLOGICAL INVESTIGATIONS Environmental Fate Ready biodegradability TEST SUBSTANCE Analogue of notified chemical METHOD International Standards Organisation method 7827 “Water Quality- Evaluation in an Aqueous Medium of the Aerobic Biodegradability of Organic Compounds, Method by Dissolved Organic Carbon” (1984) Inoculum Aerobically growing activated sludge from pilot scale sewage treatment plant Exposure Period Not stated Auxiliary Solvent None Analytical Monitoring Dissolved organic carbon determination Remarks - Method The culture medium was an aqueous solution containing several minerals and micronutrients and set to a pH of ~7. The test substance was added to the culture medium to give a DOC of 30 ppm. The medium was inoculated with 7 mL fresh aerobic activated sludge to give a suspended solids content of 30 mg/L. The culture was shaken continuously at 140- 150 rpm for the duration of the test. The temperature was maintained at 27 ± 2ºC which is outside the range specified in the method (20 to 25ºC). The biodegradation test included duplicate cultures of the test substance, a blank culture (containing only test medium and activated sludge inoculum), an inhibition test culture and an abiotic test culture. The inhibition test culture contained the test substance and sodium acetate both at levels of 30 ppm DOC. The abiotic blank culture contained the test substance in an inoculated culture that was sterilised by addition of 4 mL/L hypochlorite solution. Samples for DOC analysis (~35 mL) were withdrawn at the required intervals and centrifuged at 2,275 g for 15 min. RESULTS Test substance Sodium Acetate Day % Degradation* Day % Degradation 2 ~95% 3 >94% 7 ~98% *Information taken from a graph (DOC vs time) Remarks - Results The duration of the test was not stated, however graphical information in the paper indicates the test substance was analysed over 9 days which is adequate considering it has a measured biodegradability half life of <9.84 h. CONCLUSION The test substance and hence the notified chemical is readily biodegradable TEST FACILITY Exempt information