Appendix C: Environmental Fate and Ecotoxicological Investigations Ecotoxicological Investigations Acute toxicity to fish Notified polymer Static Acute Toxicity Test with Feathead Minnows following ABC Protocol No. 7601 Species Fathead Minnows (Pimephales promelas) Exposure Period 96 hours Auxiliary Solvent Not reported Water Hardness 130 - 142 mg CaCO3/L Analytical Monitoring None reported Remarks Method The test was conducted according to the guidelines above. However, the – study was not in compliance with good laboratory practice (GLP) principles. No significant deviations from the test guidelines were reported. The above stated test guideline is very similar to OECD TG 203. Nominal Concentration (mg/L) Number of Fish Cumulative Mortality(96 h) 48h 72h 96h 10 0 0 0 Control 100 10 0 0 0 180 10 0 0 0 320 10 0 0 0 560 10 0 0 0 1000 10 0 0 0 LC50 > 100 mg/L at 96 hours. NOEC 100 mg/L at 96 hours. Remarks Results All validity criteria for the test were satisfied. The 96 h LC 50 was – determined based on visual observations. The MSDS reported the LC50 value of the test substance was > 1000 mg/L. The notified polymer is not harmful to fish ABC (1996)
Appendix C: Environmental Fate and Ecotoxicological Investigations Ecotoxicological Investigations Acute toxicity to fish Notified polymer Static Acute Toxicity Test with Feathead Minnows following ABC Protocol No. 7601 Species Fathead Minnows (Pimephales promelas) Exposure Period 96 hours Auxiliary Solvent Not reported Water Hardness 130 - 142 mg CaCO3/L Analytical Monitoring None reported Remarks – Method The test was conducted according to the guidelines above. However, the study was not in compliance with good laboratory practice (GLP) principles. No significant deviations from the test guidelines were reported. The above stated test guideline is very similar to OECD TG 203. Nominal Concentration (mg/L) Number of Fish Cumulative Mortality(96 h) 48h 72h 96h Control 10 0 0 0 100 10 0 0 0 180 10 0 0 0 320 10 0 0 0 560 10 0 0 0 1000 10 0 0 0 LC50 > 100 mg/L at 96 hours. NOEC 100 mg/L at 96 hours. Remarks – Results All validity criteria for the test were satisfied. The 96 h LC 50 was determined based on visual observations. The MSDS reported the LC50 value of the test substance was > 1000 mg/L. The notified polymer is not harmful to fish ABC (1996)