Using the water pipette put approximately 2 mL of distilled water into the tube labeled S-. Using a clean transfer pipette, put approximately 2 mL of starch solution into the S+ test tube. Add 3 drops of Lugol’s solution to the S+ and S- tubes. Record the color in Table 1. Nucleic acids Label two microcentrifuge tubes NA+ and NA-. Using a 200 μL micropipette, put approximately 25 μL of distilled water into the microcentrifuge tube NA-. Using a p200 micropipette and clean pipette tip, add 25 μL of Lambda DNA to the NA+ tube. Using a clean pipette tip, add 25 μL of GelGreen® solution to NA+ and NA- tubes. ▲ Important: Be sure to use a new tip for each test solution. Gently mix the solutions and observe them under a UV light or blue LED light. Record the color in Table 1. ▲ Caution: Use the appropriate protective gear when using a UV light. Table 1: Macromolecule Tests Tube Macromolecule Label Description Contents Test Color Change Type Reagent Observed Sudan IV Distilled water L+ Lipids solution and vegetable oil Sudan IV Negative control, Lipids L- Distilled water solution lipids Biuret BSA solution P+ Protein solution (protein)
Using the water pipette put approximately 2 mL of distilled water into the tube labeled S-. Using a clean transfer pipette, put approximately 2 mL of starch solution into the S+ test tube. Add 3 drops of Lugol’s solution to the S+ and S- tubes. Record the color in Table 1. Nucleic acids Label two microcentrifuge tubes NA+ and NA-. Using a 200 μL micropipette, put approximately 25 μL of distilled water into the microcentrifuge tube NA-. Using a p200 micropipette and clean pipette tip, add 25 μL of Lambda DNA to the NA+ tube. Using a clean pipette tip, add 25 μL of GelGreen® solution to NA+ and NA- tubes. ▲ Important: Be sure to use a new tip for each test solution. Gently mix the solutions and observe them under a UV light or blue LED light. Record the color in Table 1. ▲ Caution: Use the appropriate protective gear when using a UV light. Table 1: Macromolecule Tests Tube Type Macromolecule Label Description Contents Test Reagent Color Change Observed Lipids L+ Lipids Distilled water and vegetable oil Sudan IV solution L- Negative control, lipids Distilled water Sudan IV solution P+ Protein BSA solution (protein) Biuret solution