Metabolic Test Substance Concentration (µg/mL) Resulting in: Activation Cytotoxicity in Cytotoxicity in Precipitation Genotoxic Effect Preliminary Test Main Test Absent Test 1 51 58 > 1842 none ≥ ≥ Test 2 51 > 120 none ≥ Present Test 1 58 58 > 1842 none ≥ ≥ Test 2 90 > 160 none ≥ Remarks - Results No statistically significant or biologically relevant increase in the number of cells with chromosome aberrations was observed in the presence or absence of metabolic activation. Positive and negative controls performed as expected. The notified chemical was not clastogenic to human lymphocytes treated under the conditions of the test. in vitro TNO (2014b)
Metabolic Activation Test Substance Concentration (µg/mL) Resulting in: Cytotoxicity in Preliminary Test Cytotoxicity in Main Test Precipitation Genotoxic Effect Absent Test 1 ≥ 51 ≥ 58 > 1842 none Test 2 ≥ 51 > 120 none Present Test 1 ≥ 58 ≥ 58 > 1842 none Test 2 ≥ 90 > 160 none Remarks - Results No statistically significant or biologically relevant increase in the number of cells with chromosome aberrations was observed in the presence or absence of metabolic activation. Positive and negative controls performed as expected. The notified chemical was not clastogenic to human lymphocytes treated in vitro under the conditions of the test. TNO (2014b)