APPENDIX A: ETHIDIUM BROMIDE WASTE HANDLING DETAILS Non-Hazardous Waste Streams Waste Stream Description Waste Management Procedure Buffer Typically contain very May be discharged to sinks. Solutions small concentrations of EtBr (< 0.01% by wt.) Gels Typically contain trace Agarose gels with trace amounts of ethidium bromide amounts of EtBr may be disposed of daily in double bag-lined μg/ml) containers (e.g., five-gallon pails). This waste is not considered a hazardous waste. However, the chemical waste vendor will pick up any vendor-supplied pails that are labeled as “Non-Hazardous Ethidium Bromide Gel Waste”. Cambridge labs that have will have to bring the pails to the mini-mains when full. Hazardous Waste Streams Contact EHS for assistance or waste removal, at 496-3797 (Cambridge) or (Longwood). Waste Stream Description Waste Management Procedure Stock Typically contain Place empty stock solution bottles or unused stock Solutions relatively high solution bottles in double ziplock plastic bags. Place concentrations of pure absorbents into bag if available. EtBr Keep waste containers/bags in secondary
APPENDIX A: ETHIDIUM BROMIDE WASTE HANDLING DETAILS Non-Hazardous Waste Streams Waste Stream Description Waste Management Procedure Buffer Solutions Typically contain very small concentrations of EtBr (< 0.01% by wt.) May be discharged to sinks. Gels Typically contain trace amounts of EtBr μg/ml) Agarose gels with trace amounts of ethidium bromide may be disposed of daily in double bag-lined containers (e.g., five-gallon pails). This waste is not considered a hazardous waste. However, the chemical waste vendor will pick up any vendor-supplied pails that are labeled as “Non-Hazardous Ethidium Bromide Gel Waste”. Cambridge labs that have will have to bring the pails to the mini-mains when full. Hazardous Waste Streams Contact EHS for assistance or waste removal, at 496-3797 (Cambridge) or (Longwood). Waste Stream Description Waste Management Procedure Stock Solutions Typically contain relatively high concentrations of pure EtBr Place empty stock solution bottles or unused stock solution bottles in double ziplock plastic bags. Place absorbents into bag if available. Keep waste containers/bags in secondary