Test material Concentration (%) Mean OD570 of Relative mean Absorbance (%) duplicate tissues Without Irradiation 100 1.286 100 Negative control Test substance 0.316 1.436 111.7 Test 1 1.0 1.093 85.0 Test 2 3.16 1.072 83.3 Test 3 10 0.912 70.9 Test 4 0.05 0.567 44.1 Positive control With Irradiation 100 1.206 100 Negative control Test substance 0.316 1.192 98.9 Test 1 1.0 1.168 96.8 Test 2 3.16 0.918 76.1 Test 3 10 0.771 63.9 Test 4 0.05 0.066 5.5 Positive control SD= Standard Deviation Remarks - Results Cytotoxic effects were not observed after exposure of the skin equivalents to the test item suspension, neither in the presence or the absence of irradiation with artificial sunlight. The controls gave responses within the expected range. The notified chemical did not exhibit any phototoxic effects on the skin equivalents under the conditions of the test. Harlan (2013)
Test material Concentration (%) Mean OD570 of duplicate tissues Relative mean Absorbance (%) Without Irradiation Negative control 100 1.286 100 Test substance Test 1 0.316 1.436 111.7 Test 2 1.0 1.093 85.0 Test 3 3.16 1.072 83.3 Test 4 10 0.912 70.9 Positive control 0.05 0.567 44.1 With Irradiation Negative control 100 1.206 100 Test substance Test 1 0.316 1.192 98.9 Test 2 1.0 1.168 96.8 Test 3 3.16 0.918 76.1 Test 4 10 0.771 63.9 Positive control 0.05 0.066 5.5 SD= Standard Deviation Remarks - Results Cytotoxic effects were not observed after exposure of the skin equivalents to the test item suspension, neither in the presence or the absence of irradiation with artificial sunlight. The controls gave responses within the expected range. The notified chemical did not exhibit any phototoxic effects on the skin equivalents under the conditions of the test. Harlan (2013)