objectives are capable of magnifications of 93x, 95x, or 100x [Note: LACC Microscopes (Biology 03).] So; what is the total magnification under oil immersion at LACC’s Biology 03 microscopes? ___________________________________________________________________________ Property Definition Once an objective has been focused, you can rotate to another objective and Parfocal the image will remain in coarse focus, requiring only slight movement of the fine focus knob. The center of the field of view remains about the same for each objective Parcentral Field of View The circle of light you see when looking through the ocular lens The space between the objective and the slide Working Distance Table 4.0 Microscopy Terms. Objective Ocular Numerical Objective Objective Magnifying Magnifying Total Magnifying Power Aperture Color Power (ObMP) Power (OcMP) (ObMP x OcMP =___x) (NA) Low Power Red – (Scanning Power) Medium Power Yellow – High Dry Blue – Oil Immersion White – (High Power) Table 4.1 Objective present on a compound Light Microscope. Property Definition The amount of the image of an object is enlarged. Magnification The quality to which objective detail in an image is preserved during Resolving Power the magnifying process. Contrast The degree to which images details stand out against their background. Table 4.2 Show some of the most important Lens and Image Properties. What is the magnification range of your Compound Light Microscope? __________________x to __________________ x Objective Magnifying Power Diameter of Field of View Low Power – (Scanning Power) Medium Power – High Dry – Oil Immersion – (High Power) Table 4.3 Diameter of Field of View.
objectives are capable of magnifications of 93x, 95x, or 100x [Note: LACC Microscopes (Biology 03).] So; what is the total magnification under oil immersion at LACC’s Biology 03 microscopes? ___________________________________________________________________________ Property Definition Parfocal Once an objective has been focused, you can rotate to another objective and the image will remain in coarse focus, requiring only slight movement of the fine focus knob. Parcentral The center of the field of view remains about the same for each objective Field of View The circle of light you see when looking through the ocular lens Working Distance The space between the objective and the slide Table 4.0 Microscopy Terms. Objective Objective Color Objective Magnifying Power (ObMP) Ocular Magnifying Power (OcMP) Total Magnifying Power (ObMP x OcMP =___x) Numerical Aperture (NA) Low – Power (Scanning Power) Red Medium – Power Yellow High – Dry Blue Oil – Immersion (High Power) White Table 4.1 Objective present on a compound Light Microscope. Property Definition Magnification The amount of the image of an object is enlarged. Resolving Power The quality to which objective detail in an image is preserved during the magnifying process. Contrast The degree to which images details stand out against their background. Table 4.2 Show some of the most important Lens and Image Properties. What is the magnification range of your Compound Light Microscope? __________________x to __________________ x Objective Magnifying Power Diameter of Field of View Low – Power (Scanning Power) Medium – Power High – Dry Oil – Immersion (High Power) Table 4.3 Diameter of Field of View.