Controlled Variables The same volume of agar mixture (20g) was put into every petri dish and left in refrigerator for 24 hours. The size of the petri dishes was the same for each therefore the surface the bacteria could cover Assumptions The temperature is the same everyday when the trials are conducted (23 Celsius). The distilled water contains the same impurities. All materials are sterilized and contain close to no bacteria. No bacteria from the surface worked on or from the breath of the one doing the experiment will contaminate the agar gel. Observations The agar jelly was a clear color before bacteria was added. Once bacteria contaminated the agar jelly the color turned yellowish. The amount of bacteria that contaminated the agar jelly changed for every petri dish therefore it was necessary to use a plastic quadrant film and compare the results before and after the essential oil was added. Once the essential oils were added the agar jelly turned a light yellow brownish color. Right away it was obvious that Wild Orange had the biggest effect on the bacteria as it resulted in the least bacterial cover among the agar jelly. DATA #1 The first trial I sterilized the petri dishes in the autoclave letting it steam for approximately 20 minutes. Then we only opened the petri dishes to put in the agar. After approximately one day all 4 of the petri dishes were utterly contaminated (100% coverture with bacteria) as you can see in the photo. The light yellow color is all the bacteria that grew. As they were contaminated I could not use them for my experiment meaning the first trial was a fail. Trial #2 For each I recorded Qualitative Results of bacterial growth without the essential oil, Qualitative Results of bacterial growth with essential oil, and the quantitative results. This trial was repeated 4 more times. Qualitative Results of bacterial growth without essential oil
Controlled Variables The same volume of agar mixture (20g) was put into every petri dish and left in refrigerator for 24 hours. The size of the petri dishes was the same for each therefore the surface the bacteria could cover Assumptions The temperature is the same everyday when the trials are conducted (23 Celsius). The distilled water contains the same impurities. All materials are sterilized and contain close to no bacteria. No bacteria from the surface worked on or from the breath of the one doing the experiment will contaminate the agar gel. Observations The agar jelly was a clear color before bacteria was added. Once bacteria contaminated the agar jelly the color turned yellowish. The amount of bacteria that contaminated the agar jelly changed for every petri dish therefore it was necessary to use a plastic quadrant film and compare the results before and after the essential oil was added. Once the essential oils were added the agar jelly turned a light yellow brownish color. Right away it was obvious that Wild Orange had the biggest effect on the bacteria as it resulted in the least bacterial cover among the agar jelly. DATA #1 The first trial I sterilized the petri dishes in the autoclave letting it steam for approximately 20 minutes. Then we only opened the petri dishes to put in the agar. After approximately one day all 4 of the petri dishes were utterly contaminated (100% coverture with bacteria) as you can see in the photo. The light yellow color is all the bacteria that grew. As they were contaminated I could not use them for my experiment meaning the first trial was a fail. Trial #2 For each I recorded Qualitative Results of bacterial growth without the essential oil, Qualitative Results of bacterial growth with essential oil, and the quantitative results. This trial was repeated 4 more times. Qualitative Results of bacterial growth without essential oil