Acute toxicity to fish Notified chemical OECD TG 203 Fish, Acute Toxicity Test Semi-Static test Species Zebra fish (Danio rerio) Exposure Period 96 hour Auxiliary Solvent None Water Hardness 135 mg CaCO3/L Analytical Monitoring HPLC Remarks Method No significant protocol deviations. – GLP Compliance 0.400g test substance was mixed with test water followed ultrasonication for 10min. This mixture was transferred into 5 L-jar and diluted with test water to 4000 mL to obtain the nominal concentration of 100 mg/L test suspension. This suspension was stirred with a magnetic stirrer for about 2 hours to get a clear solution with a nominal concentration of 100 mg/L test stock solution. A series of dilutions was made from this saturated solution to give the required test concentrations of 3.48, 5.21, 7.82, 11.7 and 17.7% nominal solutions. Concentration mg/L Number of Fish Mortality Nominal 24 h 48 h 72 h 96 h Control 10 0 0 0 0 3.48 10 0 0 0 0 5.21 10 0 0 0 0 7.82 10 0 0 0 0 11.7 10 2 3 5 7 17.6 10 10 10 10 10 LC50 10.8 mg/L at 96 hours. NOEC 7.85 mg/L at 96 hours. Remarks Results All validity criteria for the test were satisfied. – The measured concentrations of test solutions were maintained within ±20% of the nominal concentration of the test substance during the renewal period of 24 h. Therefore, the test results are based on nominal concentrations. The notified chemical is harmful to fish GCDM (2014) Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test - Static. EC Council Regulation No 440/2008 C.2 Acute Toxicity for Daphnia - Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 180 mg CaCO3/L Analytical Monitoring Ultra Performance Liquid Chromatography (UPLC) Remarks - Method No significant protocol deviations. GLP Compliance
Acute toxicity to fish Notified chemical OECD TG 203 Fish, Acute Toxicity Test Semi-Static test Species Zebra fish (Danio rerio) Exposure Period 96 hour Auxiliary Solvent None Water Hardness 135 mg CaCO3/L Analytical Monitoring HPLC Remarks – Method No significant protocol deviations. GLP Compliance 0.400g test substance was mixed with test water followed ultrasonication for 10min. This mixture was transferred into 5 L-jar and diluted with test water to 4000 mL to obtain the nominal concentration of 100 mg/L test suspension. This suspension was stirred with a magnetic stirrer for about 2 hours to get a clear solution with a nominal concentration of 100 mg/L test stock solution. A series of dilutions was made from this saturated solution to give the required test concentrations of 3.48, 5.21, 7.82, 11.7 and 17.7% nominal solutions. Concentration mg/L Number of Fish Mortality Nominal 24 h 48 h 72 h 96 h Control 3.48 10 10 0 0 0 0 0 0 0 0 5.21 10 0 0 0 0 7.82 10 0 0 0 0 11.7 17.6 10 10 2 10 3 10 5 10 7 10 LC50 10.8 mg/L at 96 hours. NOEC 7.85 mg/L at 96 hours. Remarks – Results All validity criteria for the test were satisfied. The measured concentrations of test solutions were maintained within ±20% of the nominal concentration of the test substance during the renewal period of 24 h. Therefore, the test results are based on nominal concentrations. The notified chemical is harmful to fish GCDM (2014) Acute toxicity to aquatic invertebrates Notified chemical OECD TG 202 Daphnia sp. Acute Immobilisation Test - Static. EC Council Regulation No 440/2008 C.2 Acute Toxicity for Daphnia - Species Daphnia magna Exposure Period 48 hours Auxiliary Solvent None Water Hardness 180 mg CaCO3/L Analytical Monitoring Ultra Performance Liquid Chromatography (UPLC) Remarks - Method No significant protocol deviations. GLP Compliance