Acetone (K-CHECK Tablets) (for urine, serum or plasma) PURPOSE: To define the procedure to be used for testing acetone using K-Check Reagent Tablets (Biorex Labs). POLICY: Testing of acetone will be done according to the following procedure. PRINCIPLE: Acetoacetic acid or acetone in urine or blood will form a colored complex with nitroprusside in the presence of glycine. A buffer provides the optimum pH for this reaction. REAGENTS: K-CHECK (Biorex Labs) Reagent Tablets. INSTRUMENT N/A SPECIMEN: Fresh urine, serum, plasma or whole blood. Refrigerate specimens for up to 48 hrs if cannot be tested immediately. Avoid urines with preservatives. INTERFERING SUBSTANCES Urines containing bromsulfalein, sulfhydryls, or very high quantities of phenylketones may give false positive results, as will urines preserved with 8-hydroxyquinoline. L-dopa metabolites may give an atypical reaction which could be interpreted as a positive result. QUALITY CONTROL The following should be run each time a test is run: 1, Positive; Standard (see below), 2. Negative: Distilled water. STANDARD
Acetone (K-CHECK Tablets) (for urine, serum or plasma) PURPOSE: To define the procedure to be used for testing acetone using K-Check Reagent Tablets (Biorex Labs). POLICY: Testing of acetone will be done according to the following procedure. PRINCIPLE: Acetoacetic acid or acetone in urine or blood will form a colored complex with nitroprusside in the presence of glycine. A buffer provides the optimum pH for this reaction. REAGENTS: K-CHECK (Biorex Labs) Reagent Tablets. INSTRUMENT N/A SPECIMEN: Fresh urine, serum, plasma or whole blood. Refrigerate specimens for up to 48 hrs if cannot be tested immediately. Avoid urines with preservatives. INTERFERING SUBSTANCES Urines containing bromsulfalein, sulfhydryls, or very high quantities of phenylketones may give false positive results, as will urines preserved with 8-hydroxyquinoline. L-dopa metabolites may give an atypical reaction which could be interpreted as a positive result. QUALITY CONTROL The following should be run each time a test is run: 1, Positive; Standard (see below), 2. Negative: Distilled water. STANDARD