Faculty of Health and Life Sciences COSHH RISK ASSESSMENT FORM Activity Title: Type of activity: Location of activity: Lactose practical 1 and 2 Undergraduate practical 1H4/5 or 2KH1 Assessed by (+date): Endorsed by (+date): Assessment review date: A. Ainger D Lush August 2015 August 2016 Description of experimental procedure. (Give sufficient detail so that it is clear as to the procedure being undertaken, to include substances to be used and importantly created) Ortho-nitrophenyl-beta-D-Galactopyranoside (ONPG) is used instead of lactose as a substrate for the enzyme lactase. A yellow colour is produced which can be followed at 420nm in a spectrophotometer and record the data using a laptop. Students prepare various concentrations of ONPG for session 1 to record initial rates of the reaction with a laptop attached to a spectrophotometer. In session 2 students pipette various concentrations of galactose, which is used as an inhibitor of lactose. Students record initial rates of reaction with a laptop attached to a spectrophotometer Students have an unknown galactose solution which they have to work out the concentration of. Have you used the least hazardous substances possible in this procedure in order to eliminate or minimise the hazards to health? (✓) Yes No (If No please justify ) Substance details Substance Quantity and Classification Hazard Statements Exposure (chemical, concentration (corrosive, toxic, to include exposure routes & potential Time (duration radiological or Amount in mL flammable, Biohazard health effects. / biological agents. or g then Group 1-4) From Label elements 2.2 Hazard frequency of – Including those concentration From pictogram 2.2 - Statements (H-numerical and use) created by in Mg/Ml, Please use words to description only). activity)Include %w/w, w/v, describe the pictogram. the common Molarity. See end of this form for chemical name description. from 1.1 0.1M TRIS/HCl 100mL bottle Up to 2hrs Not hazardous Not Hazardous pH 7 per 3 groups per session. 2 sessions / hydrocholoric acid containing 20mM magnesium chloride
Faculty of Health and Life Sciences COSHH RISK ASSESSMENT FORM Activity Title: Lactose practical 1 and 2 Type of activity: Undergraduate practical Location of activity: 1H4/5 or 2KH1 Assessed by (+date): A. Ainger Endorsed by (+date): D Lush August 2015 Assessment review date: August 2016 Description of experimental procedure. (Give sufficient detail so that it is clear as to the procedure being undertaken, to include substances to be used and importantly created) Ortho-nitrophenyl-beta-D-Galactopyranoside (ONPG) is used instead of lactose as a substrate for the enzyme lactase. A yellow colour is produced which can be followed at 420nm in a spectrophotometer and record the data using a laptop. Students prepare various concentrations of ONPG for session 1 to record initial rates of the reaction with a laptop attached to a spectrophotometer. In session 2 students pipette various concentrations of galactose, which is used as an inhibitor of lactose. Students record initial rates of reaction with a laptop attached to a spectrophotometer Students have an unknown galactose solution which they have to work out the concentration of. Have you used the least hazardous substances possible in this procedure in order to eliminate or minimise the hazards to health? (✓) Yes No (If No please justify ) Substance details Substance (chemical, radiological or biological agents. Including those created by activity)Include the common chemical name from 1.1 Quantity and concentration Amount in mL or g then concentration in Mg/Ml, %w/w, w/v, Molarity. Classification (corrosive, toxic, flammable, Biohazard Group 1-4) From pictogram 2.2 - Please use words to describe the pictogram. See end of this form for description. Hazard Statements to include exposure routes & potential health effects. From Label elements 2.2 – Hazard Statements (H-numerical and description only). Exposure Time (duration / frequency of use) 0.1M TRIS/HCl pH 7 / hydrocholoric acid containing 20mM magnesium chloride 100mL bottle per 3 groups Not hazardous Not Hazardous Up to 2hrs per session. 2 sessions