1:20 dilution Not a hazardous This substance is not classified as Up to 2hrs Lactase (β galactosidase) 2mL bottle substance or mixture dangerous according to Directive per session. in per group according to 67/548/EEC. 2 sessions Tris/HCl Regulation (EC) No. 0.1M buffer pH 7 + 1272/2008. 20mM magnesium chloride o-Nitrophenyl 45 mM Not hazardous Not hazardous Up to 2hrs 20mL per per session. group 2 sessions (ONPG) in Tris/HCl 0.1M buffer pH 7 containing 20mM magnesium chloride 0.666M 0.666M 10mL Not hazardous Not hazardous Up to 2hrs in Galactose in per group one session Tris/HCl 0.1M buffer pH 7 containing 20mM magnesium chloride Unknown 10mL per Not hazardous Not hazardous Up to 2hrs in concentration of group one session. Galactose in Tris/HCl 0.1M buffer pH 7 containing 20mM magnesium chloride Identify those persons at increased risk of harm (disabled, pregnancy, immuno-compromised etc) None Control measures for the activity - from Precautionary Statements 2.2 i.e.: Engineering controls e.g. use of fume cupboard etc., PPE/RPE (detail type), restricting quantity of substance used, prohibiting lone working, level of supervision, exposure monitoring, relevant SOPs. Induction of students into basic Health and Safety Previous training of students in basic laboratory skills A verbal introduction to the practical session will be delivered by a competent member of staff (academic and/or technical), during which specific risks and control measures will be highlighted to the students, including introduction of specific handling and dispensing techniques. Use of personal protective equipment (PPE) lab coat and disposable gloves. – Laboratory coat and nitrile gloves to be worn at all times. Additional special measures of control i.e. particular training in handling the substances listed or for the experimental procedure, relevant health surveillance etc None
Lactase (β galactosidase) in 0.1M Tris/HCl buffer pH 7 + 20mM magnesium chloride 1:20 dilution 2mL bottle per group Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. This substance is not classified as dangerous according to Directive 67/548/EEC. Up to 2hrs per session. 2 sessions o-Nitrophenyl (ONPG) in 0.1M Tris/HCl buffer pH 7 containing 20mM magnesium chloride 45 mM 20mL per group Not hazardous Not hazardous Up to 2hrs per session. 2 sessions 0.666M Galactose in 0.1M Tris/HCl buffer pH 7 containing 20mM magnesium chloride 0.666M 10mL per group Not hazardous Not hazardous Up to 2hrs in one session Unknown concentration of Galactose in 0.1M Tris/HCl buffer pH 7 containing 20mM magnesium chloride 10mL per group Not hazardous Not hazardous Up to 2hrs in one session. Identify those persons at increased risk of harm (disabled, pregnancy, immuno-compromised etc) None Control measures for the activity - from Precautionary Statements 2.2 i.e.: Engineering controls e.g. use of fume cupboard etc., PPE/RPE (detail type), restricting quantity of substance used, prohibiting lone working, level of supervision, exposure monitoring, relevant SOPs. Induction of students into basic Health and Safety Previous training of students in basic laboratory skills A verbal introduction to the practical session will be delivered by a competent member of staff (academic and/or technical), during which specific risks and control measures will be highlighted to the students, including introduction of specific handling and dispensing techniques. Use of personal protective equipment (PPE) – lab coat and disposable gloves. Laboratory coat and nitrile gloves to be worn at all times. Additional special measures of control i.e. particular training in handling the substances listed or for the experimental procedure, relevant health surveillance etc None