Chapter 9 DNA: The Indispensable Forensic Science Tool Learn the parts of a nucleotide and know how they are linked together to make DNA. Understand how DNA strands coil into a double helix. Describe the concept of base pairing as it relates to the double helix structure of DNA. Explain how the sequence of bases along a DNA strand ultimately determines the structure of proteins that are synthesized within the body. Describe how a double-strand DNA replicates itself. What are the implications of this process for forensic science? Understand how DNA can be cut and spliced into a foreign DNA strand. Describe some commercial applications of this recombinant DNA technology. Explain the difference between DNA strands which code for the production of proteins and those strands which contain repeating sequences of bases. Explain what is meant by a restriction fragment length polymorphism (RFLP). Describe the process of typing DNA by the RFLP technique and explain how DNA band patterns are interpreted. Explain the technology of polymerase chain reaction (PCR) and how it is applied to forensic science. Explain the latest DNA typing technique, Short Tandem Repeat (STR) analysis. Explain the difference between a traditional STR analysis and a Y-chromosome STR determination. Explain the difference between nuclear DNA and mitochondrial DNA. Discuss the application of a DNA computerized database to criminal investigation. List the necessary procedures to be taken for the proper preservation of bloodstained evidence for laboratory DNA analysis. d 1. Examples of polymers that contain repeating known as nucleotides are: a. Hemoglobin b. Starch c. Cellulose
Chapter 9 DNA: The Indispensable Forensic Science Tool Learn the parts of a nucleotide and know how they are linked together to make DNA. Understand how DNA strands coil into a double helix. Describe the concept of base pairing as it relates to the double helix structure of DNA. Explain how the sequence of bases along a DNA strand ultimately determines the structure of proteins that are synthesized within the body. Describe how a double-strand DNA replicates itself. What are the implications of this process for forensic science? Understand how DNA can be cut and spliced into a foreign DNA strand. Describe some commercial applications of this recombinant DNA technology. Explain the difference between DNA strands which code for the production of proteins and those strands which contain repeating sequences of bases. Explain what is meant by a restriction fragment length polymorphism (RFLP). Describe the process of typing DNA by the RFLP technique and explain how DNA band patterns are interpreted. Explain the technology of polymerase chain reaction (PCR) and how it is applied to forensic science. Explain the latest DNA typing technique, Short Tandem Repeat (STR) analysis. Explain the difference between a traditional STR analysis and a Y-chromosome STR determination. Explain the difference between nuclear DNA and mitochondrial DNA. Discuss the application of a DNA computerized database to criminal investigation. List the necessary procedures to be taken for the proper preservation of bloodstained evidence for laboratory DNA analysis. d 1. Examples of polymers that contain repeating known as nucleotides are: a. Hemoglobin b. Starch c. Cellulose