Amines 88 ± 1 100 ± 6 100 100 97 ± 1 106 ± 10 69.4 112.0 < 5 < 5 < 4 < 1 Isopropylamine 41 ± 2 15 ± 3 < 4 3.6 Benzylamine 64 ± 1 81 ± 5 133.9 115.6 -Alanine < 5 < 5 < 4 < 1 L-Alanine 41.0 ± 1.1 a 66.2 ± 1.0 a 165.0 10.0 D-Alanine < 5 a < 5 a n.d. < 1 L-Serine 19.6 ± 0.2 a 8.2 ± 0.7 a 9.0 6.4 < 5 a < 5 a n.d. < 1 L-Glutamate a) The reactivity of and was measured employing benzaldehyde as amino acceptor. The relative initial rate is reported L-Alanine, D-Alanine, L-Serine L-Glutamate compared to the rate of conversion of benzaldehyde with (S)-(−)-1-phenylethylamine (1.41 ± 0.04 U/mg). Initial reaction rates and final conversion with HEWT were determined respectively by the concentration of benzaldehyde and benzylamine in the reaction measured by HPLC analysis. The detection limit of both the compound is 5 %. Table 4. V. fluvialis H. elongata -TA C. violaceum -TA[36] -TA [25] Final Relative initial Conversion at Relative initial Relative initial conversion rate equilibrium rate rate (%) (%) (%) (%) (%) Ketoacids Pyruvic acid 97.5 ± 2.5 100 ± 2.2 95-100 100 100 -Hydroxypyruvic acid 39.7 ± 0.5 14.8 ± 0.3 65 144.1 29.2 -Ketoglutaric acid < 5 < 5 < 5 < 5 0.3  Aldehydes Glyoxylic acid 97.5 ± 2.5 22.5 ± 2.9 95-100 176.2 60.2 Acetaldehyde 39.2 ± 2.8 33.0 ± 0.6 n.d. n.d. 15.8 Propanal 55.1 ± 2.6 49.9 ± 4.6 60-70 132.3 36.4
Amines 88 ± 1 100 ± 6 100 100 97 ± 1 106 ± 10 69.4 112.0 < 5 < 5 < 4 < 1 Isopropylamine 41 ± 2 15 ± 3 < 4 3.6 Benzylamine 64 ± 1 81 ± 5 133.9 115.6 -Alanine < 5 < 5 < 4 < 1 L-Alanine 41.0 ± 1.1 a 66.2 ± 1.0 a 165.0 10.0 D-Alanine < 5 a < 5 a n.d. < 1 L-Serine 19.6 ± 0.2 a 8.2 ± 0.7 a 9.0 6.4 L-Glutamate < 5 a < 5 a n.d. < 1 a) The reactivity of L-Alanine, D-Alanine, L-Serine and L-Glutamate was measured employing benzaldehyde as amino acceptor. The relative initial rate is reported compared to the rate of conversion of benzaldehyde with (S)-(−)-1-phenylethylamine (1.41 ± 0.04 U/mg). Initial reaction rates and final conversion with HEWT were determined respectively by the concentration of benzaldehyde and benzylamine in the reaction measured by HPLC analysis. The detection limit of both the compound is 5 %. Table 4. H. elongata -TA C. violaceum -TA[36] V. fluvialis -TA [25] Final conversion (%) Relative initial rate (%) Conversion at equilibrium (%) Relative initial rate (%) Relative initial rate (%) Ketoacids Pyruvic acid 97.5 ± 2.5 100 ± 2.2 95-100 100 100 -Hydroxypyruvic acid 39.7 ± 0.5 14.8 ± 0.3 65 144.1 29.2  -Ketoglutaric acid < 5 < 5 < 5 < 5 0.3 Aldehydes Glyoxylic acid 97.5 ± 2.5 22.5 ± 2.9 95-100 176.2 60.2 Acetaldehyde 39.2 ± 2.8 33.0 ± 0.6 n.d. n.d. 15.8 Propanal 55.1 ± 2.6 49.9 ± 4.6 60-70 132.3 36.4