36 HPLC , affinity & gel filtration chromatography 37 Concept of reverse osmosis & ultrafiltration & electro-dialysis Handout Precipitation techniques for enzyme separation: Salting out, 38 Presentation Solvent addition, Isoelectric precipitation Introduction to intracellular separation: Cell disintegration, its 39 Lecture note importance : Factors affecting cell disruption Different types of cell disruption: Mechanical, physiochemical 40 Lecture note & biological Special emphasis to ultrasonic cell disruption & 41 homogenization as mechanical cell disintegration process, Lecture note Mathematical problems Application of enzymes for production of biochemical & food 42 Lecture note processing industries Introduction to immobilization, Its advantages, different types 43 including entrapment, micro-encapsulation, covalent bonding, Slide Presentation cross-linking Diffusional limitation to immobilized cell systems, Damkohler 44 Lecture note no Application of immobilized enzymes to food & allied 45 Slide Presentation industries, 46 Conclusion lecture Previous lecture note RECOMMENDED READINGS TEXT 1. Bioprocess Engineering: Basic Concepts, 2nd Edition- Michael L. Shulur and Fikret Kargi 2. Biochemical Engg Fundamentals-Baily, Ollis. MGH 3. Biochemical Engineering: Textbook for Engineers, Chemists and Biologists- Shigeo and Fumitake Yoshida 3. Enzyme Kinetics: A Modern Approach Alejandro G. Marangoni – 4. Enzyme Kinetics and Mechanisms- Kenneth B. Taylor REFERENCE 1. Principles of Fermentation Technology, 2nd Edition- P. F.Stanbury, A. Whitaker and S. J. Hall 2. Prescott & Dunn’s Industrial Microbiology Macmiller PAPER L C Couriol, A Amrane* and Y Prigent, An unstructured model for the analysis of substrate consumption and product release in relation to biosynthesis and cell maintenance during batch cultures of and Geotrichum candidum Penicillium camembertii. J Chem Technol 77:1300–1307 (online: 2002) Biotechnol
36 HPLC , affinity & gel filtration chromatography 37 Concept of reverse osmosis & ultrafiltration & electro-dialysis Handout 38 Precipitation techniques for enzyme separation: Salting out, Solvent addition, Isoelectric precipitation Presentation 39 Introduction to intracellular separation: Cell disintegration, its importance : Factors affecting cell disruption Lecture note 40 Different types of cell disruption: Mechanical, physiochemical & biological Lecture note 41 Special emphasis to ultrasonic cell disruption & homogenization as mechanical cell disintegration process, Mathematical problems Lecture note 42 Application of enzymes for production of biochemical & food processing industries Lecture note 43 Introduction to immobilization, Its advantages, different types including entrapment, micro-encapsulation, covalent bonding, cross-linking Slide Presentation 44 Diffusional limitation to immobilized cell systems, Damkohler no Lecture note 45 Application of immobilized enzymes to food & allied industries, Slide Presentation 46 Conclusion lecture Previous lecture note RECOMMENDED READINGS TEXT 1. Bioprocess Engineering: Basic Concepts, 2nd Edition- Michael L. Shulur and Fikret Kargi 2. Biochemical Engg Fundamentals-Baily, Ollis. MGH 3. Biochemical Engineering: Textbook for Engineers, Chemists and Biologists- Shigeo and Fumitake Yoshida 3. Enzyme Kinetics: A Modern Approach – Alejandro G. Marangoni 4. Enzyme Kinetics and Mechanisms- Kenneth B. Taylor REFERENCE 1. Principles of Fermentation Technology, 2nd Edition- P. F.Stanbury, A. Whitaker and S. J. Hall 2. Prescott & Dunn’s Industrial Microbiology Macmiller PAPER L C Couriol, A Amrane* and Y Prigent, An unstructured model for the analysis of substrate consumption and product release in relation to biosynthesis and cell maintenance during batch cultures of Geotrichum candidum and Penicillium camembertii. J Chem Technol Biotechnol 77:1300–1307 (online: 2002)