Two Dimensional Polyacrylamide Gel Electrophoresis (Two-dimensional gel electrophoresis) Two-dimensional gel electrophoresis (2-DE) is based on separating a mixture of proteins according to two molecular properties, one in each dimension. The most used is based on a first dimension separation by isoelectric focusing and second dimension according to molecular weight by SDS-PAGE . Fig. 3: Staining of 2-D gels. A) 24 cm two dimensional polyacrylamide gel electrophoresis of mouse colon protein stained by silver staining or (B) Deep purple flurophore dye. Visualization of B image was done using a laser scanner (Magdeldin et al, 2010). C, D, and E shows 2D-DIGE stained with cyDye (cy2, cy3 and cy5), respectively.
Two Dimensional Polyacrylamide Gel Electrophoresis (Two-dimensional gel electrophoresis) Two-dimensional gel electrophoresis (2-DE) is based on separating a mixture of proteins according to two molecular properties, one in each dimension. The most used is based on a first dimension separation by isoelectric focusing and second dimension according to molecular weight by SDS-PAGE . Fig. 3: Staining of 2-D gels. A) 24 cm two dimensional polyacrylamide gel electrophoresis of mouse colon protein stained by silver staining or (B) Deep purple flurophore dye. Visualization of B image was done using a laser scanner (Magdeldin et al, 2010). C, D, and E shows 2D-DIGE stained with cyDye (cy2, cy3 and cy5), respectively.