Estimate your fragment concentration using the picture of your gel and the key to the concentration of each fragment below. Is your fragment the correct length? Sequencing your Mitochondrial DNA Your samples were already mailed to GenScript, a commercial DNA sequencing facility. They use Sanger DNA sequencing, which is good for relatively short templates, such as ours. Your sequences are located in the class Blackboard folder labeled mt DNA “Class sequences”. Part IV) Analyzing your Mitochondrial DNA Sequences Trimming your mitochondrial DNA sequence 4Peaks. In order to determine how related you are to your classmates, to Rarely Reclusive, and to the human ancient and modern groups in the Dolan DNA database, you will need to first determine whether or not your sequence is of sufficient quality for the analysis, and then to trim your sequence so that only good quality data is used. The 4Peaks program that you used during your yeast experiments will work very well for this purpose. Examining the primary data of the mitochondrial control region Open up the file labeled using the program where (x) is the “mito(x).ab1” “4Peaks”, identifier for your mitochondrial DNA sequence. To do this double-click on the “4Peaks” icon, and under the pull down heading of select Then select the file name “File” “Open”. from the DNA sequences” desktop folder. This computer file “mito(x).ab1” “mitochondrial contains the sequence data we obtained from the mitochondrial control region of your mitochondrial DNA. At this point you should see peaks of four different colors. There is also an X-axis that consists of a time unit of measurement, a nucleotide number, and a nucleotide (designated A, C, G, or T). There are a few things to note:
Estimate your fragment concentration using the picture of your gel and the key to the concentration of each fragment below. Is your fragment the correct length? Sequencing your Mitochondrial DNA Your samples were already mailed to GenScript, a commercial DNA sequencing facility. They use Sanger DNA sequencing, which is good for relatively short templates, such as ours. Your sequences are located in the class Blackboard folder labeled “Class mt DNA sequences”. Part IV) Analyzing your Mitochondrial DNA Sequences Trimming your mitochondrial DNA sequence 4Peaks. In order to determine how related you are to your classmates, to Rarely Reclusive, and to the human ancient and modern groups in the Dolan DNA database, you will need to first determine whether or not your sequence is of sufficient quality for the analysis, and then to trim your sequence so that only good quality data is used. The 4Peaks program that you used during your yeast experiments will work very well for this purpose. Examining the primary data of the mitochondrial control region Open up the file labeled “mito(x).ab1” using the program “4Peaks”, where (x) is the identifier for your mitochondrial DNA sequence. To do this double-click on the “4Peaks” icon, and under the pull down heading of “File” select “Open”. Then select the file name “mito(x).ab1” from the “mitochondrial DNA sequences” desktop folder. This computer file contains the sequence data we obtained from the mitochondrial control region of your mitochondrial DNA. At this point you should see peaks of four different colors. There is also an X-axis that consists of a time unit of measurement, a nucleotide number, and a nucleotide (designated A, C, G, or T). There are a few things to note: