Figure S5. Drought tolerance A phenotype of the WT and overexpressing plants OsWNK9 before and after drought treatment. (A) The plants were grown under normal watering conditions for 3 weeks, (B) Further stressed by completely stopping of irrigation for 2 weeks (C) Phenotype of plants after rewatering for 3 weeks. Figure S6. Effect on chlorophyll content of transgenic lines and WT plants exposed Arabidopsis to different salt concentrations. The leaves of four week old plants were detached and kept in a Petri dish with different salt concentrations (100mM & 150mM) for 72 hrs and measured chlorophyll content, (A) chlorophyll a, (B) chlorophyll b. The values are presented as an average and standard error (SE) of triplicates. The asterisks represent the significant difference in chlorophyll content of transgenic lines compared with WT plants (*) P < 0.05, (**) P < 0.01. Figure S7. Hypothetical schematic model for the regulatory function of OsWNK9 under the salt and drought stress. Salt and drought stress triggered cumulatively osmotic and ionic stress which is sensed by unknown plasma membrane receptors and activate the downstream response. The probable unknown mechanisms (marked with is represented here. OsWNK9 might be ‘?’) involved in the abiotic stress responsive cascade through ABA-dependant pathways. The activated OsWNK9 further phosphorylate other unknown proteins as well as transcription of abiotic stress-related genes. It may activate vacuolar and plasma membrane transporter by phosphorylating and maintaining ion homeostasis.
Figure S5. Drought tolerance A phenotype of the WT and OsWNK9 overexpressing plants before and after drought treatment. (A) The plants were grown under normal watering conditions for 3 weeks, (B) Further stressed by completely stopping of irrigation for 2 weeks (C) Phenotype of plants after rewatering for 3 weeks. Figure S6. Effect on chlorophyll content of transgenic lines and WT Arabidopsis plants exposed to different salt concentrations. The leaves of four week old plants were detached and kept in a Petri dish with different salt concentrations (100mM & 150mM) for 72 hrs and measured chlorophyll content, (A) chlorophyll a, (B) chlorophyll b. The values are presented as an average and standard error (SE) of triplicates. The asterisks represent the significant difference in chlorophyll content of transgenic lines compared with WT plants (*) P < 0.05, (**) P < 0.01. Figure S7. Hypothetical schematic model for the regulatory function of OsWNK9 under the salt and drought stress. Salt and drought stress triggered cumulatively osmotic and ionic stress which is sensed by unknown plasma membrane receptors and activate the downstream response. The probable unknown mechanisms (marked with ‘?’) is represented here. OsWNK9 might be involved in the abiotic stress responsive cascade through ABA-dependant pathways. The activated OsWNK9 further phosphorylate other unknown proteins as well as transcription of abiotic stress-related genes. It may activate vacuolar and plasma membrane transporter by phosphorylating and maintaining ion homeostasis.