Links with sections Know how bacteria can be 1 4.3.1.1, 4.3.1.8 and or 4.3.1.9 which could be Know procedure to prepare an 10 bacteria to taught with section 4.3 uncontaminated culture. with. Infection and response. Explain why cultures are Students will require incubated at a maximum Discuss what bacteria would some knowledge of temperature of 25C. need to multiply. antibiotics before Describe why uncontaminated carrying out the cultures are necessary in Demo the techniques of Required practical. See research. producing inoculated agar sections 4.3.1.8 and plates. 4.6.3.7. Explain importance of each Bacteria multiply by step to partner or produce a simple cell division technician’s guide to (binary fission) as often inoculating plates for research. as once every 20 If you’re not doing required minutes if they have practical at this point, do enough nutrients and a further work on comparing suitable temperature. growth of bacteria on different Bacteria can be grown students’ plates. in a nutrient broth solution or as colonies Calculate the number of on an agar gel plate. bacteria in a population after a certain time given the mean Uncontaminated division time. cultures of microorganisms are
Links with sections 4.3.1.1, 4.3.1.8 and 4.3.1.9 which could be taught with section 4.3 Infection and response. Students will require some knowledge of antibiotics before carrying out the Required practical. See sections 4.3.1.8 and 4.6.3.7. Bacteria multiply by simple cell division (binary fission) as often as once every 20 minutes if they have enough nutrients and a suitable temperature. Bacteria can be grown in a nutrient broth solution or as colonies on an agar gel plate. Uncontaminated cultures of microorganisms are Know how bacteria can be Know procedure to prepare an uncontaminated culture. Explain why cultures are incubated at a maximum temperature of 25C. Describe why uncontaminated cultures are necessary in research. 1 or 10 bacteria to with. Discuss what bacteria would need to multiply. Demo the techniques of producing inoculated agar plates. Explain importance of each step to partner or produce a technician’s guide to inoculating plates for research. If you’re not doing required practical at this point, do further work on comparing growth of bacteria on different students’ plates. Calculate the number of bacteria in a population after a certain time given the mean division time.