Clinical details/ Standard Incubation Cultures read Target organism conditions Temp Time °C Gastritis Dent’s selective 35-37 Microaerobic 10 d 48hr H. pylori agar Gastric biopsy Moist chamber, or ideally containing alternative hydrogen H. pylori selective agar* Blood agar 10% 35-37 Microaerobic 10 d Every 48hr horse blood Most chamber, ideally containing hydrogen For these situations, add the following: Clinical details/ Supplementary Incubation Cultures read Target organism conditions Temp Atmos Time °C Biopsy urease Biopsy urease ambient air 24hr hourly up to 6hr H. pylori test if not already broth and again at performed in 24hr endoscopy *GC selective agar may be used in absence of H. pylori media. 2.6 Identification Refer to individual SMIs for organism identification. 2.6.1 Minimum level of identification in the laboratory species level 2.7 Antimicrobial Susceptibility Testing Refer to British Society for Antimicrobial Chemotherapy (BSAC) guidelines. 2.8 Referral to Reference Laboratories Contact appropriate reference laboratory for information on the tests available, turn around times, transport procedure and any other requirements for sample submission. Information regarding specialist and reference laboratories is available via the following websites:
Clinical details/ conditions Standard Incubation Cultures read Target organism Temp °C Time Gastritis Gastric biopsy Dent’s selective agar or alternative H. pylori selective agar* 35-37 Microaerobic Moist chamber, ideally containing hydrogen 10 d 48hr H. pylori Blood agar 10% horse blood 35-37 Microaerobic Most chamber, ideally containing hydrogen 10 d Every 48hr For these situations, add the following: Clinical details/ conditions Supplementary Incubation Cultures read Target organism Temp °C Atmos Time Biopsy urease test if not already performed in endoscopy Biopsy urease broth ambient air 24hr hourly up to 6hr and again at 24hr H. pylori *GC selective agar may be used in absence of H. pylori media. 2.6 Identification Refer to individual SMIs for organism identification. 2.6.1 Minimum level of identification in the laboratory species level 2.7 Antimicrobial Susceptibility Testing Refer to British Society for Antimicrobial Chemotherapy (BSAC) guidelines. 2.8 Referral to Reference Laboratories Contact appropriate reference laboratory for information on the tests available, turn around times, transport procedure and any other requirements for sample submission. Information regarding specialist and reference laboratories is available via the following websites: