Stimulus (type, duration, Group housing (stocking rates, Site frequency) sexes) Endpoint Isolation Animal monitoring (methods, Blood / Body Fluid Collection Shelter frequency Volume Bedding Route Hiding areas Transport Frequency Environmental enrichment Type Anaesthesia or analgesia Duration held Duration Restraint Conditioning period Confinement Animal monitoring (methods & Numbers of animals frequency) Air conditioning In-vitro Studies Source of animals Diet / Water Modifications Teaching Type Duration held Source of animals Amount Euthanasia Housing Effects Duration held Measurement of Intake Method of disposal Surgery Animal Monitoring Anaesthesia Location of pre-operative Wildlife Studies preparation area Location Surgical procedure (site, Methods Drug Treatments technique) Capture methods Substance Sterile technique (instruments, Handling methods Volume drapes, surgeon) Handling / restraint Route Location of and housing in Housing / holding Frequency / total number per recovery area Monitoring animal Post-operative management Release Local and systemic effects Post-operative monitoring Effects on population Anaesthesia or analgesia (methods, frequency, duration) Possible side effects Use of analgesics (type, dose, Restraint route, frequency, means of determining necessity for use) Expertise
Stimulus (type, duration, frequency) Group housing (stocking rates, sexes) Site Endpoint Isolation Animal monitoring (methods, Blood / Body Fluid Collection Shelter frequency Volume Bedding Route Hiding areas Transport Frequency Environmental enrichment Type Anaesthesia or analgesia Duration held Duration Restraint Conditioning period Confinement Animal monitoring (methods & frequency) Numbers of animals Air conditioning In-vitro Studies Diet / Water Modifications Source of animals Teaching Type Duration held Source of animals Amount Euthanasia Housing Effects Duration held Measurement of Intake Surgery Method of disposal Animal Monitoring Anaesthesia Location of pre-operative preparation area Wildlife Studies Location Drug Treatments Surgical procedure (site, technique) Methods Capture methods Substance Volume Sterile technique (instruments, drapes, surgeon) Handling methods Handling / restraint Route Frequency / total number per animal Location of and housing in recovery area Post-operative management Housing / holding Monitoring Release Local and systemic effects Anaesthesia or analgesia Post-operative monitoring (methods, frequency, duration) Effects on population Possible side effects Restraint Use of analgesics (type, dose, route, frequency, means of determining necessity for use) Expertise