AFP CA 125 CA 15-3 CA 19-9 Direct Direct Direct Direct Assay Chemiluminometric Chemiluminometric Chemiluminometric Chemiluminometric principle Immunoassay Immunoassay Immunoassay Immunoassay Sample Serum sample Serum sample Serum sample sample collection Transport 4°C 4°C 4°C 4°C requirement Turnaround 48 hours 48 hours 48 hours 48 hours Time Reporting – µg/L kU/L kU/L kU/L ≤8 ≤35 ≤32 ≤37 Limits 1.3 1000 µg/mL Assay Range 2 600 kU/L 0.50 200 kU/L 1.2 700 kU/L – – – (1.08 830 kU/L) – CEA ThCG Beta 2 Microglobulin SCC Direct Direct Direct Assay Chemiluminescent Chemiluminometric Chemiluminometric Chemiluminometric principle Immunoassay Immunoassay Immunoassay Immunoassay Sample Serum sample Serum sample sample Serum sample collection Transport 4°C 4°C 4°C 4°C requirement Turnaround 48 hours 48 7-10 days 72 Time Males and females: Reporting IU/L µg/L ≤2 <2.2 mg/L 70 µg/L ≤5 ≤ Limits Pregnant females: Variable Assay Range 0.5 100 µg/L 2.0 1000 IU/L Up to 20 mg/L 0 - 70 µg/L – – PSA Free PSA Testosterone Chromogranin A
AFP CA 125 CA 15-3 CA 19-9 Assay principle Direct Chemiluminometric Immunoassay Direct Chemiluminometric Immunoassay Direct Chemiluminometric Immunoassay Direct Chemiluminometric Immunoassay Sample collection Serum sample Serum sample Serum sample sample Transport requirement 4°C 4°C 4°C 4°C Turnaround Time 48 hours 48 hours 48 hours 48 hours Reporting Limits ≤8 µg/L ≤35 kU/L ≤32 kU/L ≤37 kU/L Assay Range 1.3 – 1000 µg/mL (1.08 – 830 kU/L) 2 – 600 kU/L 0.50 – 200 kU/L 1.2 – 700 kU/L CEA ThCG Beta 2 Microglobulin SCC Assay principle Direct Chemiluminometric Immunoassay Direct Chemiluminometric Immunoassay Chemiluminescent Immunoassay Direct Chemiluminometric Immunoassay Sample collection Serum sample Serum sample sample Serum sample Transport requirement 4°C 4°C 4°C 4°C Turnaround Time 48 hours 48 7-10 days 72 Reporting Limits ≤5 µg/L Males and females: ≤2 IU/L Pregnant females: Variable <2.2 mg/L ≤ 70 µg/L Assay Range 0.5 – 100 µg/L 2.0 – 1000 IU/L Up to 20 mg/L 0 - 70 µg/L PSA Free PSA Testosterone Chromogranin A