Present Test 1 0, 85, 170, 340, 680, 1360, 2720, 4 hours 7 days 14 days 0.5 DMBA, 1 DMBA Test 2 0, 85, 170, 340, 680, 1360, 2720, 4 hours 7 days 14 days 0.5 DMBA, 1 DMBA EMS, Ethyl methane sulfonate. DMBA, Dimethyl benzanthracene. Metabolic Test Substance Concentration (µg/mL) Resulting in: Activation Cytotoxicity in Cytotoxicity in Precipitation Genotoxic Effect Preliminary Test Main Test Absent Test 1 >2720 >2720 >2720 negative Test 2 >2720 >2720 >2720 negative Present Test 1 >2720 >2720 >2720 negative Test 2 >2720 >2720 >2720 negative The inseparable mixture of the notified chemicals was not clastogenic to CHO treated under the conditions of the test. in vitro Harlan (2012l)
Present Test 1 0, 85, 170, 340, 680, 1360, 2720, 0.5 DMBA, 1 DMBA 4 hours 7 days 14 days Test 2 0, 85, 170, 340, 680, 1360, 2720, 0.5 DMBA, 1 DMBA 4 hours 7 days 14 days EMS, Ethyl methane sulfonate. DMBA, Dimethyl benzanthracene. Metabolic Activation Test Substance Concentration (µg/mL) Resulting in: Cytotoxicity in Preliminary Test Cytotoxicity in Main Test Precipitation Genotoxic Effect Absent Test 1 >2720 >2720 >2720 negative Test 2 >2720 >2720 >2720 negative Present Test 1 >2720 >2720 >2720 negative Test 2 >2720 >2720 >2720 negative The inseparable mixture of the notified chemicals was not clastogenic to CHO treated in vitro under the conditions of the test. Harlan (2012l)