Color temperature Absorption Figure 3 Color temperature single factor Co 3.3 Influence of color temperature 20 0.694 Figure 3 shown the absorption of tea saponin increased 40 0.562 when the temperature rises from 40 Co to 80 Co. However, 60 1.358 the good absorption began to decline when the temperature 80 1.432 higher than 40 Co. Optimum color temperature is 40-60 Co. Table 4: The color of time Chromogenic Absorption time (min) Figure 4 Chromogenic time single factor 5 0.389 10 0.618 3.4 Influence of time 15 1.007 In order to find out the optimum time, several different time 20 1.013 tests were conducted. The results (Fig 4) showed that the tea 25 1.561 saponin absorption rate increased over time, suggesting balance point of the absorption could reach at that time, which 30 1.585 could also be the optimum time is15-25mins. 3.5 Optimization of tea saponin conditions and selection of the optimum level Orthogonal analysis (table 5) showed that the main determinants order is A>B>C>D. The higher temperature showed greatest complete reaction of the tea saponin. From the results of orthogonal experiment, the best condition for tea saponin is A3B3C2D3, at temperature is 60℃, ratio of chromogenic reagent 0.75: 3.75, 4.5, and time is 20 minutes. Table 5: An experimental program Reagent Temperature Reagent Time（min） (ml) (CO) ratio No Level 1 A1B1C1D1 3.5 40 15 0.25:4.25 2 A1B2C2D2 3.5 50 20 0.50:4.00 3 A1B3C3D3 3.5 60 25 0.75:3.75 4 A2B1C2D3 4.0 40 20 0.75:3.75 5 A2B2C3D1 4.0 50 25 0.25:4.25 6 A2B3C1D2 4.0 60 15 0.50:4.00 7 A3B1C3D2 4.5 40 25 0.50:4.00 8 A3B2C1D3 4.5 50 15 0.75:3.75 Table 6 standard curve (HPLC)
Color temperature Co Absorption 20 0.694 40 0.562 60 1.358 80 1.432 Figure 3 Color temperature single factor 3.3 Influence of color temperature Figure 3 shown the absorption of tea saponin increased when the temperature rises from 40 Co to 80 Co. However, the good absorption began to decline when the temperature higher than 40 Co. Optimum color temperature is 40-60 Co. Table 4: The color of time Chromogenic time (min) Absorption 5 0.389 10 0.618 15 1.007 20 1.013 25 1.561 30 1.585 Figure 4 Chromogenic time single factor 3.4 Influence of time In order to find out the optimum time, several different time tests were conducted. The results (Fig 4) showed that the tea saponin absorption rate increased over time, suggesting balance point of the absorption could reach at that time, which could also be the optimum time is15-25mins. 3.5 Optimization of tea saponin conditions and selection of the optimum level Orthogonal analysis (table 5) showed that the main determinants order is A>B>C>D. The higher temperature showed greatest complete reaction of the tea saponin. From the results of orthogonal experiment, the best condition for tea saponin is A3B3C2D3, at temperature is 60℃, ratio of chromogenic reagent 0.75: 3.75, 4.5, and time is 20 minutes. Table 5: An experimental program No Level Reagent (ml) Temperature (CO) Time（min） Reagent ratio 1 A1B1C1D1 3.5 40 15 0.25:4.25 2 A1B2C2D2 3.5 50 20 0.50:4.00 3 A1B3C3D3 3.5 60 25 0.75:3.75 4 A2B1C2D3 4.0 40 20 0.75:3.75 5 A2B2C3D1 4.0 50 25 0.25:4.25 6 A2B3C1D2 4.0 60 15 0.50:4.00 7 A3B1C3D2 4.5 40 25 0.50:4.00 8 A3B2C1D3 4.5 50 15 0.75:3.75 Table 6 standard curve (HPLC)