between treatment and selection): - Selection time (if incubation with a selective agent): - Fixation time (start of exposure up to fixation or harvest of cells): - Method used: agar or microwell plates for the mouse lymphoma assay. - If a selective agent is used (e.g., 6-thioguanine or trifluorothymidine), indicate its identity, its concentration and, duration and period of cell exposure. - Number of cells seeded and method to enumerate numbers of viable and mutants cells: - Criteria for small (slow growing) and large (fast growing) colonies: METHODS FOR MEASUREMENT OF CYTOTOXICITY - Method, e.g.: background growth inhibition; mitotic index (MI); relative population doubling (RPD); relative increase in cell count (RICC); replication index; cytokinesis-block proliferation index; cloning efficiency; relative total growth (RTG); relative survival (RS); other: - Any supplementary information relevant to cytotoxicity: METHODS FOR MEASUREMENTS OF GENOTOXICIY - OTHER: Rationale for test Text (2,000 Provide the rationale for selection of conditions char.) concentrations and number of cultures, including cytotoxicity data and solubility limitations, if Display: Detailed available. Evaluation Text (2,000 Describe the evaluation criteria used in the study criteria char.) to judge if a substance is positive, negative or equivocal. Display: Detailed Statistics Text (2,000 List parameters that were analysed and the char.) statistical methods used; include a statement on
between treatment and selection): - Selection time (if incubation with a selective agent): - Fixation time (start of exposure up to fixation or harvest of cells): - Method used: agar or microwell plates for the mouse lymphoma assay. - If a selective agent is used (e.g., 6-thioguanine or trifluorothymidine), indicate its identity, its concentration and, duration and period of cell exposure. - Number of cells seeded and method to enumerate numbers of viable and mutants cells: - Criteria for small (slow growing) and large (fast growing) colonies: METHODS FOR MEASUREMENT OF CYTOTOXICITY - Method, e.g.: background growth inhibition; mitotic index (MI); relative population doubling (RPD); relative increase in cell count (RICC); replication index; cytokinesis-block proliferation index; cloning efficiency; relative total growth (RTG); relative survival (RS); other: - Any supplementary information relevant to cytotoxicity: METHODS FOR MEASUREMENTS OF GENOTOXICIY - OTHER: Rationale for test conditions Text (2,000 char.) Display: Detailed Provide the rationale for selection of concentrations and number of cultures, including cytotoxicity data and solubility limitations, if available. Evaluation criteria Text (2,000 char.) Display: Detailed Describe the evaluation criteria used in the study to judge if a substance is positive, negative or equivocal. Statistics Text (2,000 char.) List parameters that were analysed and the statistical methods used; include a statement on