Filtering and culturing Three-triplicate different sample volumes were filtered using 0.2 µm filters and cultured onto mTEC and mEndo media. A total of nine replicates were performed for each sample. The incubation times and temperature were according to the manufacturers. Colony counting The green metallic colonies grown on mEndo agars were counted and the all colonies grown on mTEC agars were recorded. Statistical Analysis To test the correlation between two variables, the product-moment correlation coefficient (Pearson correlation coefficient; was used, and when significance (P) r) was found, the value accompanies the value. The statistical significance (P) was calculated P r using the T-distribution function (TDIST) for both tails. RESULTS AND DISCUSSION numbers ranged from 47-620 CFU/100 ml (145±182 CFU/100 ml; n = 9) for the upstream E. coli location, and 22-386 CFU/100 ml (152±121 CFU/100 ml) for the downstream location (Figure 3a and b). The fecal coliform was measured at 14-69 CFU/100 ml (34±21 CFU/100 ml), and CFU/100 ml (54±66 CFU/100 ml) for the upstream and downstream locations, respectively (Figure 3a and b). At the upstream location, abundance was higher than fecal coliform E. coli concentrations for all sampling events (Figure 3a). However, counts for both and fecal E. coli coliforms were not always higher at a downstream location (Figure 3b). The statistically significant Pearson correlation coefficients among total coliform bacteria from both locations and from the upstream location were found (p< 0.05). E. coli
Filtering and culturing Three-triplicate different sample volumes were filtered using 0.2 µm filters and cultured onto mTEC and mEndo media. A total of nine replicates were performed for each sample. The incubation times and temperature were according to the manufacturers. Colony counting The green metallic colonies grown on mEndo agars were counted and the all colonies grown on mTEC agars were recorded. Statistical Analysis To test the correlation between two variables, the product-moment correlation coefficient (Pearson correlation coefficient; r) was used, and when significance (P) was found, the P value accompanies the r value. The statistical significance (P) was calculated using the T-distribution function (TDIST) for both tails. RESULTS AND DISCUSSION E. coli numbers ranged from 47-620 CFU/100 ml (145±182 CFU/100 ml; n = 9) for the upstream location, and 22-386 CFU/100 ml (152±121 CFU/100 ml) for the downstream location (Figure 3a and b). The fecal coliform was measured at 14-69 CFU/100 ml (34±21 CFU/100 ml), and CFU/100 ml (54±66 CFU/100 ml) for the upstream and downstream locations, respectively (Figure 3a and b). At the upstream location, E. coli abundance was higher than fecal coliform concentrations for all sampling events (Figure 3a). However, counts for both E. coli and fecal coliforms were not always higher at a downstream location (Figure 3b). The statistically significant Pearson correlation coefficients among total coliform bacteria from both locations and E. coli from the upstream location were found (p< 0.05).