Genotoxicity Reverse Mutation Assay (Nishitomi T, 1992a) Salmonella typhimurium TA1535, TA1537, Strains: Salmonella typhimurium TA100, TA 98 and WP2 Escherichia coli uvrA liver microsomal fraction S9 from rats pretreated Metabolic activation system: with Phenobarbital (PB) and 5,6-benzoflavone (BF) mutation assay was performed twice on the same Experimetal design: bacterial strains (S. and the typhimurium E coli) following concentrations: without metabolic activation S9: treated with 2.4. 4.9, 9.8, 20, 39, 78 S. typhimurium and 156 test substance/plate g vehicle control: dimethylsulfoxide (DMSO) positive controls:2-(2-furyl) -3-(5-nitro-2-furyl) acrylamide (AF-2) Sodium azide (NaN3) 9 Aminoacridine (9AA) – treated with 313, 625, 1 250, 2 500 and E coli 5 000 test vehicle g control: DMSO positive control: N-ethyl-N’-nitro-N- nitrosoguanidine (ENNG) with metabolic activation S9: S9; treated with 4.9, 9.8, 20, 39, 78, S. typhimurium 156, 313, 625, 1 250 test g vehicle control: DMSO positive controls: Benzo [a] pyrene (BP) 2-Aminoanthracene (2-AA) treated with 313, 625, 1 250, 2 500 and E coli 5 000 test substance/plate g vehicle control: DMSO
Genotoxicity Salmonella typhimurium Reverse Mutation Assay (Nishitomi T, 1992a) Strains: Salmonella typhimurium TA1535, TA1537, TA100, TA 98 and Escherichia coli WP2 uvrA Metabolic activation system: liver microsomal fraction S9 from rats pretreated with Phenobarbital (PB) and 5,6-benzoflavone (BF) Experimetal design: mutation assay was performed twice on the same bacterial strains (S. typhimurium and E coli) the following concentrations: without metabolic activation S9: S. typhimurium treated with 2.4. 4.9, 9.8, 20, 39, 78 and 156 g test substance/plate vehicle control: dimethylsulfoxide (DMSO) positive controls:2-(2-furyl) -3-(5-nitro-2-furyl) acrylamide (AF-2) Sodium azide (NaN3) 9 – Aminoacridine (9AA) E coli treated with 313, 625, 1 250, 2 500 and 5 000 g test vehicle control: DMSO positive control: N-ethyl-N’-nitro-N- nitrosoguanidine (ENNG) with metabolic activation S9: S9; S. typhimurium treated with 4.9, 9.8, 20, 39, 78, 156, 313, 625, 1 250 g test vehicle control: DMSO positive controls: Benzo [a] pyrene (BP) 2-Aminoanthracene (2-AA) E coli treated with 313, 625, 1 250, 2 500 and 5 000 g test substance/plate vehicle control: DMSO