was slightly irritating to the conjunctiva of both the irrigated and unirrigated eyes of rabbits; irrigation diminished the severity of conjunctival irritation Draize scores (9) Time after instillation 1 hour 1 day 2 days 3 days Animal Conjunctiv rc cd de rc cd de rc cd de rc cd de a unirrigated 1 2 0 1 1 0 0 0 0 0 0 0 0 2 2 0 0 2 0 0 0 0 0 0 0 0 3 2 1 1 2 0 0 0 0 0 0 0 0 4 2 1 1 1 0 0 0 0 0 0 0 0 5 2 0 1 1 0 0 0 0 0 0 0 0 6 2 0 1 2 0 0 1 0 0 0 0 0 irrigated eye 1 2 1 0 1 0 0 1 0 0 0 0 0 2 2 1 0 1 0 0 0 0 0 0 0 0 3 2 1 1 1 0 0 0 0 0 0 0 0 see Attachment 1 for Draize scales 1 redness chemosis discharge c d e Test method: similar to OECD guidelines (8) Result: the test chemical was a slight irritant to the rabbit eye Sensitisation (7) Species/strain: guinea pig/Dunkin Hartley Number of animals: 20 test, 10 control animals Induction procedure: undiluted test chemical was applied to the exposed skin of each test animal and held in place using a semi-occlusive wrap; the wrap was removed 6 hours after exposure and excess chemical removed by rinsing; the procedure was repeated at the same site
was slightly irritating to the conjunctiva of both the irrigated and unirrigated eyes of rabbits; irrigation diminished the severity of conjunctival irritation Draize scores (9) Time after instillation Animal 1 hour 1 day 2 days 3 days Conjunctiv a rc cd de rc cd de rc cd de rc cd de unirrigated 1 2 0 1 1 0 0 0 0 0 0 0 0 2 2 0 0 2 0 0 0 0 0 0 0 0 3 2 1 1 2 0 0 0 0 0 0 0 0 4 2 1 1 1 0 0 0 0 0 0 0 0 5 2 0 1 1 0 0 0 0 0 0 0 0 6 2 0 1 2 0 0 1 0 0 0 0 0 irrigated eye 1 2 1 0 1 0 0 1 0 0 0 0 0 2 2 1 0 1 0 0 0 0 0 0 0 0 3 2 1 1 1 0 0 0 0 0 0 0 0 1 see Attachment 1 for Draize scales c redness d chemosis e discharge Test method: similar to OECD guidelines (8) Result: the test chemical was a slight irritant to the rabbit eye Sensitisation (7) Species/strain: guinea pig/Dunkin Hartley Number of animals: 20 test, 10 control animals Induction procedure: undiluted test chemical was applied to the exposed skin of each test animal and held in place using a semi-occlusive wrap; the wrap was removed 6 hours after exposure and excess chemical removed by rinsing; the procedure was repeated at the same site