1. Animals that are bred or housed Holding or weighing Survival surgery Toxicological or without any research manipulation, animals in teaching, Non‐survival surgical microbiological testing, including euthanasia. outreach or research procedures cancer research or 2. Observation of animal behavior activities Laparoscopy or needle infectious disease in the wild without manipulating Ear punching of biopsies research that requires the animal or its environment rodents continuation after clinical Retro‐orbital blood Animal behavior collection from animals signs are evident (i.e., studies without a retro-orbital without medical relief) or Imaging procedures sinus requires death as an (e.g., MRI, ultrasound, Exposure of blood endpoint x-ray) vessels for catheter 2. Severe food or water Peripheral injections, implantation deprivation beyond that blood collection or Induced infections, necessary for ordinary percutaneous catheter tumors or antibody pre‐surgical preparation implantation production (ascites 3. Application of noxious Feed studies, which do method), provided that stimuli (e.g., electrical not result in clinical animals are carefully shock) such that the health problems monitored and animal cannot Routine agricultural euthanized in a timely avoid/escape the stimuli husbandry procedures manner. and/or it is severe enough approved by the IACUC to cause more than in a protocol or SOP momentary pain or Live-trapping distress. Positive reward 4. Any procedures for training or research which needed analgesics, Tattooing tranquilizers, sedatives, Anesthesia used for or anesthetics must be restraint for a painless, or withheld for justifiable momentarily painful, study purposes procedure Euthanasia Approved by the IACUC February 18, 2010; Revised December 20, 2012, December 2013, December 2016
1. Animals that are bred or housed without any research manipulation, including euthanasia. 2. Observation of animal behavior in the wild without manipulating the animal or its environment Holding or weighing animals in teaching, outreach or research activities Ear punching of rodents Animal behavior studies Imaging procedures (e.g., MRI, ultrasound, x-ray) Peripheral injections, blood collection or percutaneous catheter implantation Feed studies, which do not result in clinical health problems Routine agricultural husbandry procedures approved by the IACUC in a protocol or SOP Live-trapping Positive reward training or research Tattooing Anesthesia used for restraint for a painless, or momentarily painful, procedure Euthanasia Survival surgery Non‐survival surgical procedures Laparoscopy or needle biopsies Retro‐orbital blood collection from animals without a retro-orbital sinus Exposure of blood vessels for catheter implantation Induced infections, tumors or antibody production (ascites method), provided that animals are carefully monitored and euthanized in a timely manner. Toxicological or microbiological testing, cancer research or infectious disease research that requires continuation after clinical signs are evident (i.e., without medical relief) or requires death as an endpoint 2. Severe food or water deprivation beyond that necessary for ordinary pre‐surgical preparation 3. Application of noxious stimuli (e.g., electrical shock) such that the animal cannot avoid/escape the stimuli and/or it is severe enough to cause more than momentary pain or distress. 4. Any procedures for which needed analgesics, tranquilizers, sedatives, or anesthetics must be withheld for justifiable study purposes Approved by the IACUC February 18, 2010; Revised December 20, 2012, December 2013, December 2016