Article Title & Link Theoretical/Conceptual Research Methodology Analysis & Results Conclusions Implications for (Add APA citations below Framework Question(s)/Hypothesis Future Research matrix Using www.citefast.com) Example Maternal germ-line Previous studies have Can mtDNA embryonic Embryonic CAPr mtDNA can be This new method This procedure introduced mtDNA stem cells be introduced stem cell transmitted through makes it possible can now be used transmission of mutant mutations into into female mice in such a culture & maternal germ line; to introduce to study several mtDNAs from embryonic systems but not in way that it will then be cybrid however, the pups did mouse mtDNA new mtDNA stem cell-derived such a way that these inherited in subsequent preparation. not live beyond into female germ mutations. chimeric mice. mutations are then maternal germ lines? Production neonatal period. line via somatic inherited via maternal & cells, resulting in lineage in subsequent Genotyping inheritance in generations. of Chimeric subsequent & generations. Transgenic Mice Pathological analysis Citations Example Sligh, J. E., Levy, S. E., Waymire, K. G., Allard, P., Dillehay, D. L., Nusinowitz, S.,Wallace, D. C. (2000). Maternal germ-line transmission of mutant mtDNAs from embryonic stem cell-derived chimeric mice. Proceedings of the National Academy of Sciences, 97(26), 14461-14466. doi:10.1073/pnas.250491597
Article Title & Link (Add APA citations below matrix Using www.citefast.com) Theoretical/Conceptual Framework Research Question(s)/Hypothesis Methodology Analysis & Results Conclusions Implications for Future Research Example Maternal germ-line transmission of mutant mtDNAs from embryonic stem cell-derived chimeric mice. Previous studies have introduced mtDNA mutations into systems but not in such a way that these mutations are then inherited via maternal lineage in subsequent generations. Can mtDNA embryonic stem cells be introduced into female mice in such a way that it will then be inherited in subsequent maternal germ lines? Embryonic stem cell culture & cybrid preparation. Production & Genotyping of Chimeric & Transgenic Mice Pathological analysis CAPr mtDNA can be transmitted through maternal germ line; however, the pups did not live beyond neonatal period. This new method makes it possible to introduce mouse mtDNA into female germ line via somatic cells, resulting in inheritance in subsequent generations. This procedure can now be used to study several new mtDNA mutations. Citations Example Sligh, J. E., Levy, S. E., Waymire, K. G., Allard, P., Dillehay, D. L., Nusinowitz, S.,Wallace, D. C. (2000). Maternal germ-line transmission of mutant mtDNAs from embryonic stem cell-derived chimeric mice. Proceedings of the National Academy of Sciences, 97(26), 14461-14466. doi:10.1073/pnas.250491597