25% 2nd Test 0 8 9 3 0 0 5 8 6 1 OX15622 in Naïve 2 5 3 0 0 2 7 1 0 0 mineral oil 12/20 15/20 Total No. of test animals with positive response: (21% OGA 499) 2.5% 2nd Test 3 0 0 0 4 1 0 0 OX15622 in mineral oil 7 3 0 0 0 8 2 0 0 0 Naïve (2.1% OGA Total No. of test animals with positive 0/20 1/20 response: 499) TABLE 2 INCIDENCE OF DERMAL RESPONSES FOLLOWING CHALLENGE (cont’) – Test substance identity & Induction Challenge Animal Incidence of Dermal Responses Result formulation details Concentration Concentration h 24 hour 48 hour 0 0.5 1 2 3 0 0.5 1 2 3 OX15623 OX15623 5% OX15623 Non- 75 wt% OGA 499 Undiluted in mineral oil Sensitising in Stoddard solvent & (75% OGA (3.8% OGA Test1 16 3 0 0 0 17 2 0 0 0 hydrotreated light distillates 499) 499) (Covance Laboratories Inc 2000b) 10 0 0 0 0 8 2 0 0 0 0/20 0/20 Total No. of test animals with positive response: OX15624 OX15624 10% OX15624 0 0 0 14 6 0 0 0 Non- 1st Test 15 5 50 wt% OGA 499 Undiluted in mineral oil Sensitising 0 0 0 8 2 0 0 0 Naive 10 0 in Stoddard solvent & (50% OGA (5% OGA 499) 0/20 0/20 Total No. of test animals with positive response: hydrotreated light distillates 499) (Covance Laboratories Inc 2000c) OGA 600 OGA 600 Test 0 12 6 1 0 OGA 2 600 13 4 1 0 39% OGA 499 in Undiluted Undiluted Stoddard solvent, hydrotreated light distillates & (39% OGA (39% OGA Naïve 0 9 1 0 0 0 9 1 0 0 10% OGA 574 499) 499) Total No. of test animals with 7/20 5/20 positive response:
25% OX15622 in mineral oil (21% OGA 499) 2nd Test 0 8 9 3 0 0 5 8 6 1 Naïve 2 5 3 0 0 2 7 1 0 0 Total No. of test animals with positive response: 12/20 15/20 2.5% OX15622 in mineral oil (2.1% OGA 499) 2nd Test 3 0 0 0 4 1 0 0 Naïve 7 3 0 0 0 8 2 0 0 0 Total No. of test animals with positive response: 0/20 1/20 TABLE 2 – INCIDENCE OF DERMAL RESPONSES FOLLOWING CHALLENGE (cont’) Test substance identity & formulation details Induction Concentration Challenge Concentration Animal Incidence of Dermal Responses Result 24 hour 48 hour 0 0.5 1 2 3 0 0.5 1 2 3 OX15623 75 wt% OGA 499 in Stoddard solvent & hydrotreated light distillates (Covance Laboratories Inc 2000b) OX15623 Undiluted (75% OGA 499) 5% OX15623 in mineral oil (3.8% OGA 499) Test1 16 3 0 0 0 17 2 0 0 0 Non- Sensitising 10 0 0 0 0 8 2 0 0 0 Total No. of test animals with positive response: 0/20 0/20 OX15624 50 wt% OGA 499 in Stoddard solvent & hydrotreated light distillates (Covance Laboratories Inc 2000c) OX15624 Undiluted (50% OGA 499) 10% OX15624 in mineral oil (5% OGA 499) 1st Test 15 5 0 0 0 14 6 0 0 0 Non- Sensitising Naive 10 0 0 0 0 8 2 0 0 0 Total No. of test animals with positive response: 0/20 0/20 OGA 600 39% OGA 499 in Stoddard solvent, hydrotreated light distillates & 10% OGA 574 OGA 600 Undiluted (39% OGA 499) OGA 600 Undiluted (39% OGA 499) Test 0 12 6 1 0 2 13 4 1 0 Naïve 0 9 1 0 0 0 9 1 0 0 Total No. of test animals with positive response: 7/20 5/20 h