This pattern imprinted on the X-ray film is called an autoradiogram When the film is developed, a pattern will emerge… What is PCR? When is it used? What are the steps of PCR. PCR (polymerase chain reaction) is a technique used to clone (amplify) DNA Step 1: Heat t0 94-96°C to separate strands Step 2: Cool to 50-65°C to anneal primers Step 3: Heat to 72°C to extend primers using Taq polymerase and Repeat How are PCR and DNA replication alike? DNA Replication PCR The 2 DNA strands are The 2 strands of DNA are separated using heat separated using enzymes At of 94°C 96 °C, the hydrogen bonds between the – helicase and gyrase complimentary strand will break, separating the strands An RNA primer must be added A DNA primer is used instead because they are easy to first before DNA nucleotides are produce in labs. added to build a One of the primers is known as a forward primer and the complementary strand to the other is a reverse primer because they start synthesis of template strand DNA in opposite directions The temp must be lowered to 50°C - 65°C in order for the primers to anneal to the template DNA Once the RNA primers have Taq polymerase a type of DNA polymerase builds – – been laid down, DNA complementary strands using DNA nucleotides that have polymerase III will build a been added to the solution complementary strand by adding DNA nucleotides What is cloning? What is the process? Cloning is the process of making identical copies genetically of organisms from specialized tissues tissues) The nucleus is taken from a specialized cell (udder cell) via nuclear transfer The nucleus is discarded from an egg cell but the egg and all contents are kept The nucleus from the specialized cell (FINN DORSET) is put into the egg cell (POLL DORSET) The egg is shocked with electricity to promote the divisions The embryo is then implanted into the uterus of another organism The fetus develops and is born.
This pattern imprinted on the X-ray film is called an autoradiogram When the film is developed, a pattern will emerge… What is PCR? When is it used? What are the steps of PCR. PCR (polymerase chain reaction) is a technique used to clone (amplify) DNA Step 1: Heat t0 94-96°C to separate strands Step 2: Cool to 50-65°C to anneal primers Step 3: Heat to 72°C to extend primers using Taq polymerase and Repeat How are PCR and DNA replication alike? DNA Replication PCR The 2 DNA strands are separated using enzymes helicase and gyrase The 2 strands of DNA are separated using heat At of 94°C – 96 °C, the hydrogen bonds between the complimentary strand will break, separating the strands An RNA primer must be added first before DNA nucleotides are added to build a complementary strand to the template strand A DNA primer is used instead because they are easy to produce in labs. One of the primers is known as a forward primer and the other is a reverse primer because they start synthesis of DNA in opposite directions The temp must be lowered to 50°C - 65°C in order for the primers to anneal to the template DNA Once the RNA primers have been laid down, DNA polymerase III will build a complementary strand by adding DNA nucleotides Taq polymerase – a type of DNA polymerase – builds complementary strands using DNA nucleotides that have been added to the solution What is cloning? What is the process? Cloning is the process of making identical copies genetically of organisms from specialized tissues tissues) The nucleus is taken from a specialized cell (udder cell) via nuclear transfer The nucleus is discarded from an egg cell but the egg and all contents are kept The nucleus from the specialized cell (FINN DORSET) is put into the egg cell (POLL DORSET) The egg is shocked with electricity to promote the divisions The embryo is then implanted into the uterus of another organism The fetus develops and is born.