Supplementary 6 Relative cell abundance at site Courseulles-sur-Mer (CS), Calais (CA) and Janssand (JS) Relative Abundance (%) CS CA JS May 2014 Probes Specificity Surface Trans. Surface Trans. EUBI-III Bacteria 95.3 92.1 94.7 90.9 94.4 ARCH915 Archaea 0.9 0.9 1.3 1.2 1.2 GAM42a Gammaproteobacteria 13.5 14.6 15.1 15.3 16.3 JTB1270 JTB255-group 6.2 5.1 5.1 3.4 5.3 GAM209 0.6 1.1 0.9 0.5 1.0 GAM1030/GAM446 2.1 2.1 1.7 0.9 3.1 Acidiferrobacter-related Note: GAM42a + JTB1270 target most of the Gammaproteobacteria (Siyambalapitiya and Blackall, 2005) Supplementary 7 Phylogenetic affiliation of fosmid endsequences recovered from a metagenomic fosmid library from shallow subsurface sediments (490 cm sediment depth), Janssand April 2004 No. of % of total archaeal and bacterial reads reads Archaea 1 60 Bacteria 3992 Bacterial and archaeal 4052 24 Gammaproteobacteria 979 Planctomycetes 12 505 Alphaproteobacteria 7 303 Deltaproteobacteria 7 301 Actinobacteria 7 289 Firmicutes 4 173 Betaproteobacteria 3 112 Bacteroidetes 2 95 Cyanobacteria 2 71 Chloroflexi 2 72 Acidobacteria 0.4 16 Other 26 Bacteria 1076 unassigned reads 5118 all reads 9170
Supplementary 6 Relative cell abundance at site Courseulles-sur-Mer (CS), Calais (CA) and Janssand (JS) Relative Abundance (%) CS CA JS May 2014 Probes Specificity Surface Trans. Surface Trans. EUBI-III Bacteria 95.3 92.1 94.7 90.9 94.4 ARCH915 Archaea 0.9 0.9 1.3 1.2 1.2 GAM42a Gammaproteobacteria 13.5 14.6 15.1 15.3 16.3 JTB1270 JTB255-group 6.2 5.1 5.1 3.4 5.3 GAM209 0.6 1.1 0.9 0.5 1.0 GAM1030/GAM446 Acidiferrobacter-related 2.1 2.1 1.7 0.9 3.1 Note: GAM42a + JTB1270 target most of the Gammaproteobacteria (Siyambalapitiya and Blackall, 2005) Supplementary 7 Phylogenetic affiliation of fosmid endsequences recovered from a metagenomic fosmid library from shallow subsurface sediments (490 cm sediment depth), Janssand April 2004 No. of reads % of total archaeal and bacterial reads Archaea 60 1 Bacteria 3992 Bacterial and archaeal 4052 Gammaproteobacteria 979 24 Planctomycetes 505 12 Alphaproteobacteria 303 7 Deltaproteobacteria 301 7 Actinobacteria 289 7 Firmicutes 173 4 Betaproteobacteria 112 3 Bacteroidetes 95 2 Cyanobacteria 71 2 Chloroflexi 72 2 Acidobacteria 16 0.4 Other Bacteria 1076 26 unassigned reads 5118 all reads 9170