Materials Needed for Complete Exercise Item Supply company Item Number Advance Preparation D. melanogaster heat shock Bloomington Drosophila Stock Center white-eyed strain (TE near 60739 () eye color gene) D. melanogaster heat shock Bloomington Drosophila Stock Center white-eyed strain 60740 (TE far from eye color gene) D. melanogaster Zimbabwe Bloomington Drosophila Stock Center 60741 (wild-type) S29 Carolina Drosophila Kit (FlyNap®, 72 vials, 72 plugs, Carolina Biological Supply () 173052 media, labels, transfer cards) Carbon Dioxide Anesthetizer Carolina Biological Supply 173034 (optional) Day 2: Inheritance and X-linkage Carolina Biological Supply Sorting Brushes (optional) 173094 Day 3: DNA Extraction Reagents needed: 10mM Tris-HCl, pH DNA Extraction Buffer 8.2; 1mM EDTA; 25mM NaCl Reagents needed: 0.02g Proteinase-K (Sigma Aldrich: P-6556); 0.6ml 10mM Proteinase K (20 mg/mL) Tris-HCl, pH 7.6; 0.4ml glycerol Proteinase K Solution (20 New England BioLabs, Inc. mg/mL) P8107S () (Alternative to above) 1.7-ml Microcentrifuge Genesee Scientific 22-281 tubes () 0.2-ml 8-strip PCR tubes Genesee Scientific 27-125 Day 3: PCR Oligonucleotide Primers (see below for IDT DNA () specifications) 0.2-ml 8-strip PCR tubes Genesee Scientific 27-125 DNA Taq Polymerase New England BioLabs, Inc. M0267S 10X ThermoPol PCR buffer New England BioLabs, Inc. M0267S (20mM Mg++) Deoxynucleotide Set Bioline USA Inc. BIO-39025
Materials Needed for Complete Exercise Item Supply company Item Number Advance Preparation D. melanogaster heat shock white-eyed strain (TE near eye color gene) Bloomington Drosophila Stock Center () 60739 D. melanogaster heat shock white-eyed strain (TE far from eye color gene) Bloomington Drosophila Stock Center 60740 D. melanogaster Zimbabwe (wild-type) S29 Bloomington Drosophila Stock Center 60741 Carolina Drosophila Kit (FlyNap®, 72 vials, 72 plugs, media, labels, transfer cards) Carolina Biological Supply () 173052 Carbon Dioxide Anesthetizer (optional) Carolina Biological Supply 173034 Day 2: Inheritance and X-linkage Sorting Brushes (optional) Carolina Biological Supply 173094 Day 3: DNA Extraction DNA Extraction Buffer Reagents needed: 10mM Tris-HCl, pH 8.2; 1mM EDTA; 25mM NaCl Proteinase K (20 mg/mL) Reagents needed: 0.02g Proteinase-K (Sigma Aldrich: P-6556); 0.6ml 10mM Tris-HCl, pH 7.6; 0.4ml glycerol Proteinase K Solution (20 mg/mL) (Alternative to above) New England BioLabs, Inc. () P8107S 1.7-ml Microcentrifuge tubes Genesee Scientific () 22-281 0.2-ml 8-strip PCR tubes Genesee Scientific 27-125 Day 3: PCR Oligonucleotide Primers (see below for specifications) IDT DNA () 0.2-ml 8-strip PCR tubes Genesee Scientific 27-125 DNA Taq Polymerase New England BioLabs, Inc. M0267S 10X ThermoPol PCR buffer (20mM Mg++) New England BioLabs, Inc. M0267S Deoxynucleotide Set Bioline USA Inc. BIO-39025