Buffer ____ μL Polymerase ____ μL dNTPs ____ μL Primers ____ μL Water ____ μL Total _50_ μL List the three phases of a PCR cycle, what happens in each phase, and the relative temperature at which this occurs. Design the forward and reverse primers (20 base long) you would use to PCR amplify this DNA sequence from your favorite organism. Assume that you want to amplify sequence shown here (i.e. your primers the entire should start at the very edges of the sequence below). 5’ ATTAGTCTAGAAATTCGCGACGTAGTCAGCA - 3’ – 3’ TAATCAGATCTTTAAGCGCTGCATCAGTCGT - 5’ – More on your Final Project At this point we have had a good bit of discussion about the “abstraction hierarchy” in synthetic biology, particularly focusing on Now it’s time to devices.
Buffer ____ μL Polymerase ____ μL dNTPs ____ μL Primers ____ μL Water ____ μL Total _50_ μL List the three phases of a PCR cycle, what happens in each phase, and the relative temperature at which this occurs. Design the forward and reverse primers (20 base long) you would use to PCR amplify this DNA sequence from your favorite organism. Assume that you want to amplify the entire sequence shown here (i.e. your primers should start at the very edges of the sequence below). 5’ – ATTAGTCTAGAAATTCGCGACGTAGTCAGCA - 3’ 3’ – TAATCAGATCTTTAAGCGCTGCATCAGTCGT - 5’ More on your Final Project At this point we have had a good bit of discussion about the “abstraction hierarchy” in synthetic biology, particularly focusing on devices. Now it’s time to