Test 1 2500 > 5000 2500 ≥ ≥ Test 2 1000 > 5000 1000 ≥ ≥ Present ≥ 2500 Test 1 > 5000 ≥ 2500 ≥ 2500 Test 2 1000 > 5000 1000 ≥ ≥ Remarks - Results The plates incubated with the test substance showed reduced background growth in nearly all strains with and without metabolic activation in both independent experiments. Toxic effects, evident as a reduction in the number of revertants (below the indication factor of 0.5), occurred in nearly all strains in both experiments. No substantial increase in revertant colony numbers of any of the five tester strains was observed following treatment with Hostapon SG at any dose level, neither in the presence nor absence of metabolic activation (S9 mix). There was also no tendency of higher mutation rates with increasing concentrations in the range below the generally acknowledged border of biological relevance. Appropriate reference mutagens were used as positive controls. They showed a distinct increase of induced revertant colonies, thus confirming the efficiency of the test system. The test substance was not mutagenic to bacteria under the conditions of the test. Harlan Laboratories Ltd (2008e) Appendix C: Environmental Fate and Ecotoxicological Investigation Environmental Fate Ready biodegradability Notified chemical (~25% liquid particle dispersion) OECD TG 301 B Ready Biodegradability: CO2 Evolution Test (Modified Sturm Test) Inoculum Activated sludge suspension derived from a municipal sewage treatment plant treating domestic sewage Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring pH, CO2, Temperature, Aeration Remarks - Method Minor changes from the guideline: The mineral nutrient solution was directly prepared in the test vessels. The pH of the mineral nutrient solution was checked and adjusted to pH 7.4 ± 0.2 if necessary. The titration of barium hydroxide solution was performed potentiometrically instead of by visual titration against phenolphthalein indicator. Test substance Sodium benzoate Day % degradation Day % degradation 9 67 9 Not evaluated 28 84.5 28 Not evaluated Remarks Results The test substance is classified as ready biodegradable as the pass level – (60% ThCO2) was reached in a 10-day window within the 28-day period of the test. The complete evolved CO2 for the reference substance
Test 1 ≥ 2500 > 5000 ≥ 2500 Test 2 ≥ 1000 > 5000 ≥ 1000 Present ≥ 2500 Test 1 ≥ 2500 > 5000 ≥ 2500 Test 2 ≥ 1000 > 5000 ≥ 1000 Remarks - Results The plates incubated with the test substance showed reduced background growth in nearly all strains with and without metabolic activation in both independent experiments. Toxic effects, evident as a reduction in the number of revertants (below the indication factor of 0.5), occurred in nearly all strains in both experiments. No substantial increase in revertant colony numbers of any of the five tester strains was observed following treatment with Hostapon SG at any dose level, neither in the presence nor absence of metabolic activation (S9 mix). There was also no tendency of higher mutation rates with increasing concentrations in the range below the generally acknowledged border of biological relevance. Appropriate reference mutagens were used as positive controls. They showed a distinct increase of induced revertant colonies, thus confirming the efficiency of the test system. The test substance was not mutagenic to bacteria under the conditions of the test. Harlan Laboratories Ltd (2008e) Appendix C: Environmental Fate and Ecotoxicological Investigation Environmental Fate Ready biodegradability Notified chemical (~25% liquid particle dispersion) OECD TG 301 B Ready Biodegradability: CO2 Evolution Test (Modified Sturm Test) Inoculum Activated sludge suspension derived from a municipal sewage treatment plant treating domestic sewage Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring pH, CO2, Temperature, Aeration Remarks - Method Minor changes from the guideline: The mineral nutrient solution was directly prepared in the test vessels. The pH of the mineral nutrient solution was checked and adjusted to pH 7.4 ± 0.2 if necessary. The titration of barium hydroxide solution was performed potentiometrically instead of by visual titration against phenolphthalein indicator. Test substance Sodium benzoate Day % degradation Day % degradation 9 67 9 Not evaluated 28 84.5 28 Not evaluated Remarks – Results The test substance is classified as ready biodegradable as the pass level (60% ThCO2) was reached in a 10-day window within the 28-day period of the test. The complete evolved CO2 for the reference substance