In each case New Zealand white rabbits were administered single dermal doses of 10 000 mg/kg of test substance. Test Substance Comment LD50 C12-C14 olefinH14 - >10 000 mg/kg C12 - C26* 3/sex >10 000 mg/kg Olefin/ParaffinH18 One animal died on Day 9 showing hyporeactivity and diarrhoea. Weight loss observed in 75% of animals. No pathology seen. C14 C16* OlefinH16 6 animals, male and female. >10 000 mg/kg – 24 hour exposure. C14 C18 OlefinH16 6 animals, male and female. >10 000 mg/kg – 24 hour exposure. C14 C18* Olefin/Paraffin 3/sex. 24 hour exposure. All gained >10 000 mg/kg – H18 weight satisfactorily. No mortality. No pathology seen. C14 C26* 3/sex. 24 hour exposure. All gained >10 000 mg/kg – Olefin/ParaffinH18 weight satisfactorily. No mortality. C16-C18H16 6 animals, male and female. 24 hour >10 000 mg/kg exposure. C18 C24* Olefins H18 3/sex. All gained weight satisfactorily. >10 000 mg/kg – No pathology seen. No mortality. C18 C26* 3/sex. All gained weight satisfactorily. >10 000 mg/kg – Olefin/ParaffinH18 No pathology seen. No mortality. C18 C30* Olefin/Alcohol 3/sex. All gained weight satisfactorily. >10 000 mg/kg – H18 No pathology seen. No mortality. * Purity not specified. H14 (EU 1995) H16 (EU 1995) H18 (EU 1995) 9.1.3 Inhalation Toxicity 9.1.3.1 Acute Inhalation Toxicity of C12-C16 Alpha Olefin (Rinehart 1967) C12-C16 alpha Olefin (98.5% olefins, 1.5% saturates) Test Substance: Rat/Wistar Species/strain:
In each case New Zealand white rabbits were administered single dermal doses of 10 000 mg/kg of test substance. Test Substance Comment LD50 C12-C14 olefinH14 - >10 000 mg/kg C12 - C26* Olefin/ParaffinH18 3/sex One animal died on Day 9 showing hyporeactivity and diarrhoea. Weight loss observed in 75% of animals. No pathology seen. >10 000 mg/kg C14 – C16* OlefinH16 6 animals, male and female. 24 hour exposure. >10 000 mg/kg C14 – C18 OlefinH16 6 animals, male and female. 24 hour exposure. >10 000 mg/kg C14 – C18* Olefin/Paraffin H18 3/sex. 24 hour exposure. All gained weight satisfactorily. No mortality. No pathology seen. >10 000 mg/kg C14 – C26* Olefin/ParaffinH18 3/sex. 24 hour exposure. All gained weight satisfactorily. No mortality. >10 000 mg/kg C16-C18H16 6 animals, male and female. 24 hour exposure. >10 000 mg/kg C18 – C24* Olefins H18 3/sex. All gained weight satisfactorily. No pathology seen. No mortality. >10 000 mg/kg C18 – C26* Olefin/ParaffinH18 3/sex. All gained weight satisfactorily. No pathology seen. No mortality. >10 000 mg/kg C18 – C30* Olefin/Alcohol H18 3/sex. All gained weight satisfactorily. No pathology seen. No mortality. >10 000 mg/kg * Purity not specified. H14 (EU 1995) H16 (EU 1995) H18 (EU 1995) 9.1.3 Inhalation Toxicity 9.1.3.1 Acute Inhalation Toxicity of C12-C16 Alpha Olefin (Rinehart 1967) Test Substance: C12-C16 alpha Olefin (98.5% olefins, 1.5% saturates) Species/strain: Rat/Wistar