Perform: The process steps will vary An aerosol may be Moderate Required: Low according to the created during Vaginal wet mounts Follow written procedures. microscopic exam being pipetting, Wear PPE: lab coat, gloves and additional KOH/calcoflour/India ink performed. cytocentrifuging, eye protection when there is a risk of a preparations centrifuging, splash or spray. Gram stains List your test specific uncapping or If AFB stain/culture is ordered on a specimen, process here. preparing the slide Use cytospin to perform all work, including Possible spill or splash cytocentrifugation, in a class II BSC using prepare Gram of specimen BSL-3 practices (controlled access to the AFB stains area when working, decontamination of all Use cytospin to waste, must wear a solid front gown with prepare AFB on cuffed sleeves, gloves and respirator/PAPR). sterile body fluid Dry slides in the BSC before removing. Additional Protection - Preferred: Viral immunofluorescent Perform all work in a BSC. (IF) stains Prepare cytospin slide in a BSC. If cytospin Use cytospin to cannot be operated in a BSC, open the prepare viral IF centrifuged cytospin removable sealed stain rotor in a BSC. Allow slides to dry in the BSC before removing for staining. Cell and differential Lab has mechanism in place for notification of Urinalysis possible high risk infectious agents. Use additional BSL-3 practices when patient is or suspected to have an infectious disease transmitted by aerosols or droplets (i.e. MERS, SARS, avian influenza, mumps, measles, etc.). Additional Protection - Alternative: Work behind a full safety shield/face shield and wear eye protection. Do not flame slides, but allow slides to air dry or dry on a heating block/slide dryer prior to fixing and staining. Comments: Direct Microscopic Exam: (continued)
Perform: Vaginal wet mounts KOH/calcoflour/India ink preparations Gram stains Use cytospin to prepare Gram AFB stains Use cytospin to prepare AFB on sterile body fluid Viral immunofluorescent (IF) stains Use cytospin to prepare viral IF stain Cell and differential Urinalysis The process steps will vary according to the microscopic exam being performed. List your test specific process here. An aerosol may be created during pipetting, cytocentrifuging, centrifuging, uncapping or preparing the slide Possible spill or splash of specimen Moderate Required: Follow written procedures. Wear PPE: lab coat, gloves and additional eye protection when there is a risk of a splash or spray. If AFB stain/culture is ordered on a specimen, perform all work, including cytocentrifugation, in a class II BSC using BSL-3 practices (controlled access to the area when working, decontamination of all waste, must wear a solid front gown with cuffed sleeves, gloves and respirator/PAPR). Dry slides in the BSC before removing. Additional Protection - Preferred: Perform all work in a BSC. Prepare cytospin slide in a BSC. If cytospin cannot be operated in a BSC, open the centrifuged cytospin removable sealed rotor in a BSC. Allow slides to dry in the BSC before removing for staining. Lab has mechanism in place for notification of possible high risk infectious agents. Use additional BSL-3 practices when patient is or suspected to have an infectious disease transmitted by aerosols or droplets (i.e. MERS, SARS, avian influenza, mumps, measles, etc.). Additional Protection - Alternative: Work behind a full safety shield/face shield and wear eye protection. Do not flame slides, but allow slides to air dry or dry on a heating block/slide dryer prior to fixing and staining. Low Comments: Direct Microscopic Exam: (continued)