Draize (8) Scoresii Animal Time after 1 Hour 3 days 7 instillation 1 day 2 days days opacity opacity opacity opacity opacit y CORNEA: area area area area area 1 0 1* 0 2* 0 0 0 0 0 0 2 0 1* 0 0 0 0 0 0 0 0 3 0 1* 0 1* 0 1* 0 0 0 0 IRIS 1 1 0 0 0 0 2 0 0 0 0 0 3 0 0 0 0 0 ra dc ra dc ra dc ra cb dc ra cb dc CONJUNCTIVA c 1 1 2 0 2 1 1 2 1 1 1 1 1 1 1 0 2 1 2 0 2 1 1 1 1 1 1 1 0 1 0 1 b 3 1 2 0 2 1 1 1 1 1 1 0 0 1 0 0 *stained area redness chemosis discharge a Skin Sensitisation (10) Result: Strong sensitiser Species/strain: Albino female guinea-pigs/ Number of animals: 20 in test group, AlpK:Dunkin Hartley 10 in control group Induction: Injections of 5% (w/w) notified chemical in saline; FCA, 1:1 with distilled water; and 10% (w/w) notified chemical in saline with 1:1 FCA in distilled water. Topical induction at day 8: 25% (w/v) notified chemical in vaseline. Results: Challenge 24 hrs 48 hrs Concentration test control test control 0% 0/20 0/10 0/20 0/10 5% 14/20 0/10 8/20 0/10 10% 10/20 0/10 18/20 0/10 25% 15/20 3/10 16/20 3/10 Positive responses varied from scattered mild redness to intense reddening and swelling. 18 animals showed a positive reaction in response to the 10% concentration. This results indicate a sensitisation rate of 90% Test Method: directive 84/449/EEC (5) Test B6
Draize (8) Scoresii Animal 1 Hour Time after instillation 1 day 2 days 3 days 7 days CORNEA: opacity area opacity area opacity area opacity area opacit area y 1 0 1* 0 2* 0 0 0 0 0 0 2 0 1* 0 0 0 0 0 0 0 0 3 0 1* 0 1* 0 1* 0 0 0 0 IRIS 1 1 0 0 0 0 2 0 0 0 0 0 3 0 0 0 0 0 CONJUNCTIVA ra dc ra dc ra dc ra cb dc ra cb dc 1 1 2 0 2 1 1 2 1 1 1 1 1 1 1 0 2 1 2 0 2 1 1 1 1 1 1 1 0 1 0 1 3 1 2 0 2 1 1 1 1 1 1 0 0 1 0 0 *stained area a redness b chemosis c discharge Skin Sensitisation (10) Result: Strong sensitiser Species/strain: Albino female guinea-pigs/ Number of animals: 20 in test group, AlpK:Dunkin Hartley 10 in control group Induction: Injections of 5% (w/w) notified chemical in saline; FCA, 1:1 with distilled water; and 10% (w/w) notified chemical in saline with 1:1 FCA in distilled water. Topical induction at day 8: 25% (w/v) notified chemical in vaseline. Results: Challenge Concentration 24 hrs test control 48 hrs test control 0% 0/20 0/10 0/20 0/10 5% 14/20 0/10 8/20 0/10 10% 10/20 0/10 18/20 0/10 25% 15/20 3/10 16/20 3/10 Positive responses varied from scattered mild redness to intense reddening and swelling. 18 animals showed a positive reaction in response to the 10% concentration. This results indicate a sensitisation rate of 90% Test Method: directive 84/449/EEC (5) Test B6