PCR Activities Performed” column is not to be a detailed description, simply a “Research quick reference of activities being performed for biosafety staff. Recombinant DNA (rDNA) and Synthetic Nucleic Acid Molecule Information: Section III-B. Please note any intermediate host(s) prior to final host(s) in Column 2. If a significant number of plasmids exist in a given project, a supplemental spreadsheet may be submitted with the protocol form in lieu of completing Table III-B to list said plasmids. Sec. III-B Example: If to be harmful (e.g. Oncogenic, What is the gene or Toxic, Mutated Gene) transcription product Plasmid and/or to researcher or Source Species of Vector(s) to be environment, please inserted DNA Host(s) to be used used describe: Human E.coli Plasmid - pSC101, cDNA, microRNA, pMB9, siRNA, shRNA, (HBA1, pBR322 HBA2, HBB) for iron binding in hemoglobin. Fish Human Cells Lentiviral Vector, Blue Fluorescent Gene (HBA1, protein (EBFP, EBFP2, HBA2, HBB) for Azurite) iron binding in hemoglobin. Biohazards: Section V. Sec. V Table Example: Biological Material: Potential Routes of Transmission (check [X] all applicable routes ) (Micro)Organism; Blood or other Bloodborne mucous Pathogens material; Level/Biosafety Human Cells/Cell Lines; (RG) (Y/N) Non-Human Primate and animal fluids, cells or contact contact wound (BL) Group tissuesI; Source Infectious Host Biological Toxin Range membranes Direct Direct Risk Containment open Zoonotic? Level Inoculation Ingestion Inhalation RG2 BL2 X X X X X P.aeruginosa ATCC Humans, animals
PCR “Research Activities Performed” column is not to be a detailed description, simply a quick reference of activities being performed for biosafety staff. Section III-B. Recombinant DNA (rDNA) and Synthetic Nucleic Acid Molecule Information: Please note any intermediate host(s) prior to final host(s) in Column 2. If a significant number of plasmids exist in a given project, a supplemental spreadsheet may be submitted with the protocol form in lieu of completing Table III-B to list said plasmids. Sec. III-B Example: Source Species of inserted DNA Host(s) to be used Plasmid and/or Vector(s) to be used What is the gene or transcription product If to be harmful (e.g. Oncogenic, Toxic, Mutated Gene) to researcher or environment, please describe: Human E.coli Plasmid - pSC101, pMB9, pBR322 cDNA, microRNA, siRNA, shRNA, (HBA1, HBA2, HBB) for iron binding in hemoglobin. Fish Human Cells Lentiviral Vector, Gene (HBA1, HBA2, HBB) for iron binding in hemoglobin. Blue Fluorescent protein (EBFP, EBFP2, Azurite) Section V. Biohazards: Sec. V Table Example: Biological Material: (Micro)Organism; Blood or other Bloodborne Pathogens material; Human Cells/Cell Lines; Non-Human Primate and animal fluids, cells or tissuesI; Biological Toxin Source Infectious Host Range Zoonotic? (Y/N) Risk Group (RG) Containment Level/Biosafety Level (BL) Potential Routes of Transmission (check [X] all applicable routes ) Inoculation Ingestion Inhalation Direct contact open wound Direct contact mucous membranes P.aeruginosa ATCC Humans, animals RG2 BL2 X X X X X