AORTA M1 vs M2 panel- Cyan Marker (cell type) Fluorophore Channel Dilutions Clone/Company YFP (SMCs) FITC FL1 2uL/100uL endogenous ????????? PE FL2 ??????? CD45 (hematopoeitic) PercP FL4 0.5uL/100uL Clone M1/70 Cat#101216 CD11b (pan macrophage) PE-Cy7 FL5 1uL/100uL Biolegend CD86 (M1 macrophage) Pacific Blue FL6 1uL/100uL Cat #105022 Biolegend Live/dead Yellow FL7 0.6uL/300uL Clone:BM8 Cat#17480180 F4/80 (pan macrophage) APC FL8 0.5uL/100uL eBioscience Clone N418 Cat#47011482 CD11c (dendritic cells) FL9 1uL/100uL eBioscience Clone 93 Cat#14-016185 FC Block (CD16/CD32) None None 1uL/100uL eBioscience
AORTA M1 vs M2 panel- Cyan Marker (cell type) Fluorophore Channel Dilutions Clone/Company YFP (SMCs) FITC FL1 2uL/100uL endogenous ????????? PE FL2 ??????? CD45 (hematopoeitic) PercP FL4 0.5uL/100uL CD11b (pan macrophage) PE-Cy7 FL5 1uL/100uL Clone M1/70 Cat#101216 Biolegend CD86 (M1 macrophage) Pacific Blue FL6 1uL/100uL Cat #105022 Biolegend Live/dead Yellow FL7 0.6uL/300uL F4/80 (pan macrophage) APC FL8 0.5uL/100uL Clone:BM8 Cat#17480180 eBioscience CD11c (dendritic cells) FL9 1uL/100uL Clone N418 Cat#47011482 eBioscience FC Block (CD16/CD32) None None 1uL/100uL Clone 93 Cat#14-016185 eBioscience