the notified chemical was non mutagenic the conditions Result: of the test 9.3.3 Micronucleus Assay in the Bone Marrow Cells of the Mouse (Daughtrey & Bird, 1995) mouse/CD-1 Species/strain: 15/sex/dose Number and sex of animals: 0.15, 0.375, 0.75 g/kg Doses: intraperitoneal injection, vehicle corn oil; dose volume < 10 Method of administration: mL/kg cyclophosphamide 40 mg/kg Positive control: not stated Test method: in a range finding study, all mice treated at 5 g/kg, one at 2.5 Comment: g/kg and two at 1.0 g/kg died; all mice at lower doses survived for 3 days; 0.75 g/kg was chosen as the highest non-toxic dose bone marrow was collected 24, 48 and 72 hours after administration no increase in micronucleus frequency in polychromatic erythrocytes at any dose or collection time was observed; no signs of overt marrow toxicity measured by a significant decrease in the percentage of polychromatic erythrocytes was observed the positive control caused large, statistically significant increases in micronucleus frequency, indicating that the test system responded appropriately the notified chemical was non clastogenic under the Result: conditions of the test Developmental and Reproductive Toxicity 9.4.1 Two Generation Reproductive Toxicity (American Petroleum Institute, 1998) rat/CD (Sprague-Dawley) Species/Strain:
Result: the notified chemical was non mutagenic the conditions of the test 9.3.3 Micronucleus Assay in the Bone Marrow Cells of the Mouse (Daughtrey & Bird, 1995) Species/strain: mouse/CD-1 Number and sex of animals: 15/sex/dose Doses: 0.15, 0.375, 0.75 g/kg Method of administration: intraperitoneal injection, vehicle corn oil; dose volume < 10 mL/kg Positive control: cyclophosphamide 40 mg/kg Test method: not stated Comment: in a range finding study, all mice treated at 5 g/kg, one at 2.5 g/kg and two at 1.0 g/kg died; all mice at lower doses survived for 3 days; 0.75 g/kg was chosen as the highest non-toxic dose bone marrow was collected 24, 48 and 72 hours after administration no increase in micronucleus frequency in polychromatic erythrocytes at any dose or collection time was observed; no signs of overt marrow toxicity measured by a significant decrease in the percentage of polychromatic erythrocytes was observed the positive control caused large, statistically significant increases in micronucleus frequency, indicating that the test system responded appropriately Result: the notified chemical was non clastogenic under the conditions of the test Developmental and Reproductive Toxicity 9.4.1 Two Generation Reproductive Toxicity (American Petroleum Institute, 1998) Species/Strain: rat/CD (Sprague-Dawley)