solution to be tested. Carefully wipe the optically clear sides of the cuvettes with a Kimwipe or lens paper to remove any drops of liquid, dust, or fingerprints. Open the sample compartment. Insert the cuvette in the sample holder. Orient the cuvette A 1/10 dilution such that the is a good optically clear sides place to start. are aligned with the Use the blank light path. solution to dilute the the sample sample. If the compartment. absorbance is still too high, Press the softkey dilute again labeled and repeat “Measure blank”. The display until an should read 0.000A. accurate measurement Remove the blank is obtained. cuvette and insert Then, multiply a cuvette filled with the your sample. The absorbance instrument will reading by the display the dilution factor measured to obtain the absorbance. absorbance of the original If the absorbance is concentration. greater than 2.0, the display will show an message Never use any stating that the abrasive sample is outside cleansers, the range of the soaps, or instrument. This will brushes on require you to dilute cuvettes. This the sample to gain can scratch an accurate them or leave reading. a
solution to be tested. Carefully wipe the optically clear sides of the cuvettes with a Kimwipe or lens paper to remove any drops of liquid, dust, or fingerprints. Open the sample compartment. Insert the cuvette in the sample holder. Orient the cuvette such that the optically clear sides are aligned with the light path. the sample compartment. Press the softkey labeled “Measure blank”. The display should read 0.000A. Remove the blank cuvette and insert a cuvette filled with your sample. The instrument will display the measured absorbance. If the absorbance is greater than 2.0, the display will show an message stating that the sample is outside the range of the instrument. This will require you to dilute the sample to gain an accurate reading. A 1/10 dilution is a good place to start. Use the blank solution to dilute the sample. If the absorbance is still too high, dilute again and repeat until an accurate measurement is obtained. Then, multiply the absorbance reading by the dilution factor to obtain the absorbance of the original concentration. Never use any abrasive cleansers, soaps, or brushes on cuvettes. This can scratch them or leave a