Concentration mg/L Number of D. magna Number Immobilised Nominal 24 h 48 h 6.25 20 0 0 12.5 20 0 0 25 20 0 0 50 20 0 0 100 20 0 0 EC50 >100 mg/L at 48 hours Remarks Results The test criterion of the acute toxicity is immobilization of the daphnids – after 48 hours of exposure which is expressed as effect concentration (EC). None of the test organisms was immobilised after 48 h of exposure at concentration up to 100 mg/L. In the control, immobilisation was Thus, the validity criteria were fulfilled by the test. ≤10%. The notified chemical is considered to be very slightly to Daphnia (Mensink et al 1995). magna BASF (1995d) 8.2.3. Algal growth inhibition test Notified chemical EEC Directive 79/831/EEC, Annex V, Part C; Algae: Growth inhibition test, updated May 1998. Species algae) Scenedesmus subspicatus Exposure Period 72 hours Concentration Nominal 0.39 mg/L 100 mg/L – Auxiliary Solvent None Water Hardness Not reported Analytical Monitoring GC with mass selective detection Remarks Method Three replicates per each test concentration and three replicates for the – control were used. In vivo chlorophyll-a fluorescence was measured after 0, 24, 48 and 72 h. Cell counts were performed after 72 h in a counting chamber. The EC values were calculated from the concentration-response curve. Biomass Growth Nominal EbC50 Nominal Nominal ErC50 Nominal NOErC (mg/L) at 72 h (mg/L) at 72 h (mg/L) at 72 h (mg/L) at 72 h >100 mg/L mg/L >100 mg/L mg/L ≥100 ≥100 Remarks Results The 72 h LOEC of >100 mg/L is determined by comparing the of – the fluorescence measurement of the various concentrations with the control. The NOEC (72 h) is the test concentration immediately below the LOEC and is determined to be mg/L. The validity criteria were ≥100 maintained. The notified chemical is considered to be very slightly toxic to algae (Mensink et al 1995). BASF (1995e)
Concentration mg/L Number of D. magna Number Immobilised Nominal 24 h 48 h 6.25 20 0 0 12.5 20 0 0 25 20 0 0 50 20 0 0 100 20 0 0 EC50 >100 mg/L at 48 hours Remarks – Results The test criterion of the acute toxicity is immobilization of the daphnids after 48 hours of exposure which is expressed as effect concentration (EC). None of the test organisms was immobilised after 48 h of exposure at concentration up to 100 mg/L. In the control, immobilisation was ≤10%. Thus, the validity criteria were fulfilled by the test. The notified chemical is considered to be very slightly to Daphnia magna (Mensink et al 1995). BASF (1995d) 8.2.3. Algal growth inhibition test Notified chemical EEC Directive 79/831/EEC, Annex V, Part C; Algae: Growth inhibition test, updated May 1998. Species Scenedesmus subspicatus algae) Exposure Period 72 hours Concentration Nominal 0.39 mg/L – 100 mg/L Auxiliary Solvent None Water Hardness Not reported Analytical Monitoring GC with mass selective detection Remarks – Method Three replicates per each test concentration and three replicates for the control were used. In vivo chlorophyll-a fluorescence was measured after 0, 24, 48 and 72 h. Cell counts were performed after 72 h in a counting chamber. The EC values were calculated from the concentration-response curve. Biomass Growth Nominal EbC50 Nominal Nominal ErC50 Nominal NOErC (mg/L) at 72 h (mg/L) at 72 h (mg/L) at 72 h (mg/L) at 72 h >100 mg/L ≥100 mg/L >100 mg/L ≥100 mg/L Remarks – Results The 72 h LOEC of >100 mg/L is determined by comparing the of the fluorescence measurement of the various concentrations with the control. The NOEC (72 h) is the test concentration immediately below the LOEC and is determined to be ≥100 mg/L. The validity criteria were maintained. The notified chemical is considered to be very slightly toxic to algae (Mensink et al 1995). BASF (1995e)