Appendix C: Environmental Fate and Ecotoxicological Investigations Environmental Fate Ready biodegradability Notified polymer OECD TG 301 B Ready Biodegradability: CO2 Evolution Test. Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring Determination of carbon dioxide by chemical titration Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles. As the test substance was difficult to dissolve in water, stock solution was not prepared. The test substance (61 mg) was directly added to the test system. The degradation was determined by the amount of production of carbon dioxide. Test substance Sodium benzoate Day % Degradation Day % Degradation 3 0 14 75 7 3.2 10 13 14 20 20 25 28 26 Remarks - Results The percentage degradation of the reference compound (sodium benzoate) was determined to be 75% after 14 days, passed the threshold level of 60% for ready biodegradability. Therefore, the tests indicate the suitability of the inoculums. The toxicity control showed 48% biodegradation on day 14, indicating the notified chemical did not inhibit the microbial degradation of the reference substance. The notified polymer is not considered to be readily biodegradable. LEES (2009) Ready biodegradability Notified polymer Method for Testing the Biodegradability of Chemical Substances by Microorganisms (Testing Methods for New Chemical Substances, No.1121002, Ministry of Environment, Japan) Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring Biochemical oxygen demand (BOD) measured with an oxygen measuring apparatus Test substance measured with HPLC Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles.
Appendix C: Environmental Fate and Ecotoxicological Investigations Environmental Fate Ready biodegradability Notified polymer OECD TG 301 B Ready Biodegradability: CO2 Evolution Test. Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring Determination of carbon dioxide by chemical titration Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles. As the test substance was difficult to dissolve in water, stock solution was not prepared. The test substance (61 mg) was directly added to the test system. The degradation was determined by the amount of production of carbon dioxide. Test substance Sodium benzoate Day % Degradation Day % Degradation 3 0 14 75 7 3.2 10 13 14 20 20 25 28 26 Remarks - Results The percentage degradation of the reference compound (sodium benzoate) was determined to be 75% after 14 days, passed the threshold level of 60% for ready biodegradability. Therefore, the tests indicate the suitability of the inoculums. The toxicity control showed 48% biodegradation on day 14, indicating the notified chemical did not inhibit the microbial degradation of the reference substance. The notified polymer is not considered to be readily biodegradable. LEES (2009) Ready biodegradability Notified polymer Method for Testing the Biodegradability of Chemical Substances by Microorganisms (Testing Methods for New Chemical Substances, No.1121002, Ministry of Environment, Japan) Inoculum Activated sludge Exposure Period 28 days Auxiliary Solvent None Analytical Monitoring Biochemical oxygen demand (BOD) measured with an oxygen measuring apparatus Test substance measured with HPLC Remarks - Method Conducted in accordance with the test guidelines above, and in compliance with GLP standards and principles.