The results are presented in the below. Incubation Components Counts per Minute cDNA probe plus primary RNA transcripts from liver-cell nuclei 15,000 cDNA probe plus primary RNA transcripts from brain-cell nuclei 150 of the following statements concerning The regulation of the expression of mRNA in the DNA sequence corresponding to this liver the isolated nuclei from the two cell types as specific mRNA molecule is best supported by demonstrated in this experiment is an the data? example of It is transcribed in brain-cell nuclei only. translational control It is transcribed in both brain- and mRNA degradation control nuclei at approximately the same rate. transcriptional control It is not transcribed in brain-cell nuclei but transport control is transcribed in liver-cell nuclei. lack of control It is not transcribed in either liver- or brain- cell nuclei. It may be transcribed in both brain- and liver- cell nuclei but cannot be -detected by this experimental procedure. To produce radiolabeled transcripts, the isolated nuclei from both liver and brain must have which of the following? DNA polymerase RNA polymerase Reverse transcriptase Ribosomes Plasmid DNA To make the single-stranded cDNA used in this experiment, which of the following enzymes was required? DNA polymerase Reverse transcriptase DNase Protease Protein kinase Questions 177-180 Two types of preparations from leaves were used to determine whether photosynthesis can provide energy for synthesis of a particular chloroplast protein (CP). Identical amounts of amino acids were introduced into 14C preparations of whole-cell homogenates and of isolated chloroplasts, each containing equal quantities of chlorophyll. As indicated in the table below, reactions were carried out in light or dark. After each reaction was terminated, labeled CP was isolated and counted. Additives used in treatments 5, 6, and 7 were supplied before the introduction of amino acids. 14C
The results are presented in the below. Incubation Components Counts per Minute cDNA probe plus primary RNA transcripts from liver-cell nuclei 15,000 cDNA probe plus primary RNA transcripts from brain-cell nuclei 150 of the following statements concerning the DNA sequence corresponding to this liver specific mRNA molecule is best supported by the data? It is transcribed in brain-cell nuclei only. It is transcribed in both brain- and nuclei at approximately the same rate. It is not transcribed in brain-cell nuclei but is transcribed in liver-cell nuclei. It is not transcribed in either liver- or brain- cell nuclei. It may be transcribed in both brain- and liver- cell nuclei but cannot be -detected by this experimental procedure. To produce radiolabeled transcripts, the isolated nuclei from both liver and brain must have which of the following? DNA polymerase RNA polymerase Reverse transcriptase Ribosomes Plasmid DNA To make the single-stranded cDNA used in this experiment, which of the following enzymes was required? DNA polymerase Reverse transcriptase DNase Protease Protein kinase The regulation of the expression of mRNA in the isolated nuclei from the two cell types as demonstrated in this experiment is an example of translational control mRNA degradation control transcriptional control transport control lack of control Questions 177-180 Two types of preparations from leaves were used to determine whether photosynthesis can provide energy for synthesis of a particular chloroplast protein (CP). Identical amounts of 14C amino acids were introduced into preparations of whole-cell homogenates and of isolated chloroplasts, each containing equal quantities of chlorophyll. As indicated in the table below, reactions were carried out in light or dark. After each reaction was terminated, labeled CP was isolated and counted. Additives used in treatments 5, 6, and 7 were supplied before the introduction of 14C amino acids.