9.1.1 Oral Toxicity (Safepharm Laboratories Limited Rat/Sprague-Dawley Species/strain: 5/sex Number/sex of animals: 14 days Observation period: 5 000 mg/kg by gavage in a dose volume of 6.28 mL/kg Method of administration: OECD TG 401; EC Method B1 Test method: Nil Mortality: were no clinical signs of systemic toxicity. Clinical observations: No abnormalities were noted at necropsy. Morphological findings: > 5 000 mg/kg Estimated LD50: Alkane 2 was of very low acute oral toxicity to the rat. Result: 9.1.2 Dermal Toxicity (Safepharm Laboratories Limited 1995c) Rat/Sprague-Dawley CD Species/strain: 5/sex Number/sex of animals: 14 days Observation period: A single, 24-hour semi occluded dermal application to intact Method of administration: skin at a dose level of 2 000 mg/kg bw, dose volume of 2.51 mL/kg. OECD TG 402; EC Method B3. Test method: Nil Mortality: No clinical signs of systemic toxicity were noted. Clinical observations: No signs of dermal irritation were noted. Dermal response: No abnormalities were noted at necropsy. Morphological findings: > 2 000 mg/kg bw LD50: Alkane 2 was of low dermal toxicity to the rat. Result:
9.1.1 Oral Toxicity (Safepharm Laboratories Limited Species/strain: Rat/Sprague-Dawley Number/sex of animals: 5/sex Observation period: 14 days Method of administration: 5 000 mg/kg by gavage in a dose volume of 6.28 mL/kg Test method: OECD TG 401; EC Method B1 Mortality: Nil Clinical observations: were no clinical signs of systemic toxicity. Morphological findings: No abnormalities were noted at necropsy. Estimated LD50: > 5 000 mg/kg Result: Alkane 2 was of very low acute oral toxicity to the rat. 9.1.2 Dermal Toxicity (Safepharm Laboratories Limited 1995c) Species/strain: Rat/Sprague-Dawley CD Number/sex of animals: 5/sex Observation period: 14 days Method of administration: A single, 24-hour semi occluded dermal application to intact skin at a dose level of 2 000 mg/kg bw, dose volume of 2.51 mL/kg. Test method: OECD TG 402; EC Method B3. Mortality: Nil Clinical observations: No clinical signs of systemic toxicity were noted. Dermal response: No signs of dermal irritation were noted. Morphological findings: No abnormalities were noted at necropsy. LD50: > 2 000 mg/kg bw Result: Alkane 2 was of low dermal toxicity to the rat.