Gene Mutations and Proteins Name: ______________________________ Period _________ To demonstrate how gene mutations affect the production of proteins Objective: Procedure: 1. Use the following base sequence of one strand of an imaginary DNA molecule: TACCTACATTTTCCTGAA 2. Write the base sequence for an mRNA strand that would be transcribed from the given DNA sequence. Place your results in the table below. 3. Use the genetic code to determine the sequence of amino acids in the resulting protein fragment. Place your results in the table below. 4. If the fourth base in the original DNA strand were changed from C to G, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. 5. If G were added to the original DNA strand after the third base, what would the resulting mRNA look like? How would this addition affect the protein? Show your results in the table below. 6. If the 10th base in the original DNA strand were changed from T to A, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. If the 6th base in the original DNA strand were changed from A to G, how would this affect the 7. resulting protein fragment? Write the new protein fragment in the table below. mRNA from Step 2 Protein Sequence from Step 3 Protein Sequence from Step 4 MRNA from Step 5 Protein Sequence from Step 5 Protein Sequence from Step 6 Protein Sequence from Step 7 Conclusions: 1. Which change in DNA was a point mutation? (in which step did it occur?) 2. In what way did the point mutation affect the protein? Which was a frameshift mutation?
Gene Mutations and Proteins Name: ______________________________ Period _________ Objective: To demonstrate how gene mutations affect the production of proteins Procedure: 1. Use the following base sequence of one strand of an imaginary DNA molecule: TACCTACATTTTCCTGAA 2. Write the base sequence for an mRNA strand that would be transcribed from the given DNA sequence. Place your results in the table below. 3. Use the genetic code to determine the sequence of amino acids in the resulting protein fragment. Place your results in the table below. 4. If the fourth base in the original DNA strand were changed from C to G, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. 5. If G were added to the original DNA strand after the third base, what would the resulting mRNA look like? How would this addition affect the protein? Show your results in the table below. 6. If the 10th base in the original DNA strand were changed from T to A, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. 7. If the 6th base in the original DNA strand were changed from A to G, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. mRNA from Step 2 Protein Sequence from Step 3 Protein Sequence from Step 4 MRNA from Step 5 Protein Sequence from Step 5 Protein Sequence from Step 6 Protein Sequence from Step 7 Conclusions: 1. Which change in DNA was a point mutation? (in which step did it occur?) 2. In what way did the point mutation affect the protein? Which was a frameshift mutation?