Micropropagation technique, factors affecting culture of plants (physical, chemical, in vitro – genotypic and others), applications and limitations of micropropagation. Meristem, shoot tip culture and production of virus free plants. Somaclonal variations, molecular basis of variation and their significance in plant breeding. Unit - III production of haploid plants Androgenesis (anther and pollen culture) and Gynogenesis In vitro – (ovary and ovule culture). Significance and uses of haploids in agriculture. Wide hybridization and embryo rescue technique. Unit - IV Protoplast culture and somatic hybridization Isolation, culture and fusion of protoplast, – selection of fusion products and plant regeneration, assessment of somatic hybrid plants, production of cybrids, applications of protoplast culture and somatic hybridization in the improvement of crop plants. germplasm conservation and cryopreservation. In vitro Recommended Books: 1. tissue culture Theory and Practice (2005) by Bhojwani S. S. and Razdan M. K., – Elsevier publication. 2. Elements of Biotechnology by P. K. Gupta, Rastogi pub. 3. Biotechnology in crop improvement (1998) by H. S. Chawla, International Book distributing company. 4. cell, organ and tissue culture (1995) by Gamborg O.L. and Phillips G.C., Springer Verlag pub. Germany. 5. Plant Tissue Culture Basic & Applied (2005) by Jha T.B. & Ghosh B., Universities press. – 6. cell culture A practical approach (1994) Dixon R.A., Gonzales R.A. Oxford University – press, UK. 7. Bhojwani S.S. (2003), Agrobiotechnology & Plant Tissue Culture 8. Smith R.H. (2000), Plant Tissue Culture, Academic Press 9. Evans D.A. (2003), Plant Cell Culture, Taylor & Francis Semester - II
Micropropagation – technique, factors affecting in vitro culture of plants (physical, chemical, genotypic and others), applications and limitations of micropropagation. Meristem, shoot tip culture and production of virus free plants. Somaclonal variations, molecular basis of variation and their significance in plant breeding. Unit - III In vitro production of haploid plants – Androgenesis (anther and pollen culture) and Gynogenesis (ovary and ovule culture). Significance and uses of haploids in agriculture. Wide hybridization and embryo rescue technique. Unit - IV Protoplast culture and somatic hybridization – Isolation, culture and fusion of protoplast, selection of fusion products and plant regeneration, assessment of somatic hybrid plants, production of cybrids, applications of protoplast culture and somatic hybridization in the improvement of crop plants. In vitro germplasm conservation and cryopreservation. Recommended Books: 1. tissue culture – Theory and Practice (2005) by Bhojwani S. S. and Razdan M. K., Elsevier publication. 2. Elements of Biotechnology by P. K. Gupta, Rastogi pub. 3. Biotechnology in crop improvement (1998) by H. S. Chawla, International Book distributing company. 4. cell, organ and tissue culture (1995) by Gamborg O.L. and Phillips G.C., Springer Verlag pub. Germany. 5. Plant Tissue Culture – Basic & Applied (2005) by Jha T.B. & Ghosh B., Universities press. 6. cell culture – A practical approach (1994) Dixon R.A., Gonzales R.A. Oxford University press, UK. 7. Bhojwani S.S. (2003), Agrobiotechnology & Plant Tissue Culture 8. Smith R.H. (2000), Plant Tissue Culture, Academic Press 9. Evans D.A. (2003), Plant Cell Culture, Taylor & Francis Semester - II