EXERCISE 4: EXPERIEMNTATION IN CELL CULTURES We are going to do in vitro cell biology studies In Vitro Experimental Methods and Design. this semester. cell biology studies are those that utilize an alternating The more classic In Vitro combination design. Identify a natural activity that interests you as a researcher. Use In Vivo in vivo – system to screen for the possible molecular players. Go back and check for false positives and in vitro real possibilities Use your system to test your positives. The ultimate goal is to repeat in vivo. in vitro test in vivo. 1. The Concept of the Bioassay a. The bioassay uses a natural biological activity as the measurable endpoint to the experiment. b. Bioassays are models of an biological activity. vivo” “in
EXERCISE 4: EXPERIEMNTATION IN CELL CULTURES In Vitro Experimental Methods and Design. We are going to do in vitro cell biology studies this semester. The more classic cell biology studies are those that utilize an alternating In Vitro – In Vivo combination design. Identify a natural activity in vivo that interests you as a researcher. Use in vitro system to screen for the possible molecular players. Go back and check for false positives and real possibilities in vivo. Use your in vitro system to test your positives. The ultimate goal is to repeat test in vivo. 1. The Concept of the Bioassay a. The bioassay uses a natural biological activity as the measurable endpoint to the experiment. b. Bioassays are models of an “in vivo” biological activity.