Figure 4.31 The percentage of control cells and RAMP3 knockdown cells 207 which were labelled with DID staining on days 0, 3, 7, 10, 14 and 17. Figure 4.32 The migration shows the motility and the ability of the 208 control cells and the RAMP3 knock down cells to close a wound made in the monolayer of the cells in 72h Figure 4.33 A bar graph representing the percentage of wound closure by 209 control virus cells and RAMP3 knock down cells in 72 hours. Figure 4.34 Stimulating the control virus and RAMP3 knock down cells 210 migration by using AM in dose dependent manner. Figure 4.35 Inhibiting the control virus and RAMP3 knock down cells 211 migration by AM22-52. Figure 4.36 The invasion of the control cells and the RAMP3 knock 212 down cells for 48h through basement membrane transwell. Figure 4.37 The optical density (OD) of control virus and RAMP3 knock down 213 cells that invaded the basement membrane transwell. Figure 4.38 The invasion of control virus cells and RAMP3 knock down cells 214 to the transwell. Figure 4.39 The clonogenic assay of control virus cells and RAMP3 knock 216 down cells. Figure 4.40 The viable cells number of control virus and RAMP3 knock down 218 cells after 5FU treatment. The number of dead cells of control virus cells and RAMP3 knock 219 Figure 4.41 down cells after 5FU treatment. Figure 4.42 The cAMP assay of RAMP3 knock down cells and control virus 220 cells. Figure 4.43 The percentage of cAMP in RAMP3 knock down cells and control 221 virus cells. Figure 5.1 The schematic represents the in vivo experimental plan. 237 Figure 5.2 The average weight of the tumours 240 Figure 5.3 The effect of knocking down RAMP3 on the tumour formation in 241 129S6/SvEv.
Figure 4.31 The percentage of control cells and RAMP3 knockdown cells which were labelled with DID staining on days 0, 3, 7, 10, 14 and 17. 207 Figure 4.32 The migration shows the motility and the ability of the control cells and the RAMP3 knock down cells to close a wound made in the monolayer of the cells in 72h 208 Figure 4.33 A bar graph representing the percentage of wound closure by control virus cells and RAMP3 knock down cells in 72 hours. 209 Figure 4.34 Stimulating the control virus and RAMP3 knock down cells migration by using AM in dose dependent manner. 210 Figure 4.35 Inhibiting the control virus and RAMP3 knock down cells migration by AM22-52. 211 Figure 4.36 The invasion of the control cells and the RAMP3 knock down cells for 48h through basement membrane transwell. 212 Figure 4.37 The optical density (OD) of control virus and RAMP3 knock down cells that invaded the basement membrane transwell. 213 Figure 4.38 The invasion of control virus cells and RAMP3 knock down cells to the transwell. 214 Figure 4.39 The clonogenic assay of control virus cells and RAMP3 knock down cells. 216 Figure 4.40 The viable cells number of control virus and RAMP3 knock down cells after 5FU treatment. 218 Figure 4.41 The number of dead cells of control virus cells and RAMP3 knock down cells after 5FU treatment. 219 Figure 4.42 The cAMP assay of RAMP3 knock down cells and control virus cells. 220 Figure 4.43 The percentage of cAMP in RAMP3 knock down cells and control virus cells. 221 Figure 5.1 The schematic represents the in vivo experimental plan. 237 Figure 5.2 The average weight of the tumours 240 Figure 5.3 The effect of knocking down RAMP3 on the tumour formation in 129S6/SvEv. 241