Clinical Specimen Standard Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C S. aureus Swab carriage 5-10% Blood agar 35-37 16-24hr 16hr S. aureus For CO2 example Boils Lancefield Swab group A 5-10% Lancefield group A Blood agar 35-37 16-24hr 16hr strep CO2 streptococcus carriage Clinical Specimen Supplementary Incubation Cultures Target organism(s) details/ media read Temp Atmos Time conditions °C Antral washout, Oncology sinus 5-10% Sabaroud plate 35-37 16-24hr 40hr Fungi patients aspirate CO2 and sinus washout Antral Chocolate agar* 35-37 5-10% 40-48hr β-haemolytic washout, CO2 streptococci sinus aspirate Enterobacteriaceae and sinus H. influenzae washout M.catarrhalis Pseudomonads S. aureus S. anginosis group S. pneumoniae Sinusitis Antral Fastidious 35-37 5-7 d Fusobacterium ≥48hr washout, anaerobe agar species sinus with 5µg Peptostreptococcus aspirate metronidazole species and sinus disc washout Propionibacterium species Prevotella species Antral washout, ≥40hr 30 and Sabouraud Agar Air 5 d and up to aspirate 35-37 5 d and sinus washout *may include either a bacitracin 10 unit disc or bacitracin incorporated in the agar. If microscopy is suggestive of organisms not listed in target list additional and/or other media may be required. 4.6 Identification
Clinical details/ conditions Specimen Standard media Incubation Cultures read Target organism(s) Temp °C Atmos Time S. aureus carriage For example Boils Swab Blood agar 35-37 5-10% CO2 16-24hr 16hr S. aureus Lancefield group A strep carriage Swab Blood agar 35-37 5-10% CO2 16-24hr 16hr Lancefield group A streptococcus Clinical details/ conditions Specimen Supplementary media Incubation Cultures read Target organism(s) Temp °C Atmos Time Oncology patients Antral washout, sinus aspirate and sinus washout Sabaroud plate 35-37 5-10% CO2 16-24hr 40hr Fungi Sinusitis Antral washout, sinus aspirate and sinus washout Chocolate agar* 35-37 5-10% CO2 40-48hr β-haemolytic streptococci Enterobacteriaceae H. influenzae M.catarrhalis Pseudomonads S. aureus S. anginosis group S. pneumoniae Antral washout, sinus aspirate and sinus washout Fastidious anaerobe agar with 5µg metronidazole disc 35-37 5-7 d ≥48hr Fusobacterium species Peptostreptococcus species Propionibacterium species Prevotella species Antral washout, aspirate and sinus washout Sabouraud Agar 30 and 35-37 Air 5 d ≥40hr and up to 5 d *may include either a bacitracin 10 unit disc or bacitracin incorporated in the agar. If microscopy is suggestive of organisms not listed in target list additional and/or other media may be required. 4.6 Identification