Dermal Species Strain, age, Sex Route Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference information weight Rat Wistar M & F Dermal 2000 mg/kg bw (moistened but Distilled Results: Alves, M.C. Hannover undiluted) water & There were no deaths, behavioural or clinical (2005b) (Rattus gauze Test material purity: 98.2% alterations. No treatment-related macroscopic norvegicus), 10-Nov-2005 alterations were observed during the necropsies. n = 5/sex/treatment Adult albino (Report-No: RF - Exposure Area: ~10% of the total 0030.310.299.05 body surface area Conclusion: Document No.: 8–14 wk Exposure time: 24 h The dermal LD50 for methiocarb is > 2000 mg/kg bw. M-261741-01-1) Observation period: 14 d M: 254–278 g F: 221–269 g Skin irritation Species Strain, age Sex Route Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference information weight Rabbit NZ White M & F Dermal 0.5 g of technical material Distilled Results: Collor, L.V.M. (moistened but undiluted) water & Young adult The test item applied on the skin of the rabbits did not (2005a) gauze Test material: methiocarb purity cause any dermal irritation. patch 23-Sept-2005 (98.2%) 13–16 wk (Report-No: RF - n = 3 (2M, 1F) No treatment-related behavioural or clinical alterations 0030.311.318.05 Exposure Area: ~6 cm2 were noted during the observation period. 2.968–3.417 kg Document No.: Exposure time: 4 h M-261812-01-1)
Dermal Species Strain, age, weight Sex Route Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference information Rat Wistar Hannover (Rattus norvegicus), Adult albino 8–14 wk M: 254–278 g F: 221–269 g M & F Dermal 2000 mg/kg bw (moistened but undiluted) Test material purity: 98.2% n = 5/sex/treatment Exposure Area: ~10% of the total body surface area Exposure time: 24 h Observation period: 14 d Distilled water & gauze Results: There were no deaths, behavioural or clinical alterations. No treatment-related macroscopic alterations were observed during the necropsies. Conclusion: The dermal LD50 for methiocarb is > 2000 mg/kg bw. Alves, M.C. (2005b) 10-Nov-2005 (Report-No: RF - 0030.310.299.05 Document No.: M-261741-01-1) Skin irritation Species Strain, age weight Sex Route Test parameters (e.g. dose) Vehicle Toxicology conclusion/s Reference information Rabbit NZ White Young adult 13–16 wk 2.968–3.417 kg M & F Dermal 0.5 g of technical material (moistened but undiluted) Test material: methiocarb purity (98.2%) n = 3 (2M, 1F) Exposure Area: ~6 cm2 Exposure time: 4 h Distilled water & gauze patch Results: The test item applied on the skin of the rabbits did not cause any dermal irritation. No treatment-related behavioural or clinical alterations were noted during the observation period. Collor, L.V.M. (2005a) 23-Sept-2005 (Report-No: RF - 0030.311.318.05 Document No.: M-261812-01-1)