Changing the project PI Contact EH&S at for the next steps. PART ONE. Facilities List each UW research space where you will perform work with biohazardous agents. Identify specific buildings, rooms, and activities. In vitro Use Building/Room Activities Biohazardous Agents Comments Health Sciences Building, Cell culture of human cells, AAV, plasmids, human cells, BSL-2 tissue culture room. growth of lentiviral vectors, transgenic plant seeds, Certified biosafety cabinet in T287 creation of transgenic plants Pseudomonas aeruginosa room. EXAMPLE Animal Use Building/Room Activities Biohazardous Agents Comments Health Sciences Building, (e.g., implanting human cells (e.g., human cell lines, BSL-2 tissue culture room. in mice, perfusions of mice murine cells transduced with Certified biosafety cabinet in T287 exposed to reovirus, housing gammaretroviral vectors) room. EXAMPLE of exposed animals) Shared Core Facilities (e.g., MRI, FACS, hESC, UW Botany Greenhouse) Facility/Building/Room Activities Biohazardous Agents Comments MRI / Brotman B67 (e.g., cell sorting, imaging of (e.g., cell lines, animal cells EXAMPLE animals, flow cytometry) from exposed animals, cells with recombinant DNA)
Changing the project PI Contact EH&S at for the next steps. PART ONE. Facilities List each UW research space where you will perform work with biohazardous agents. Identify specific buildings, rooms, and activities. In vitro Use Building/Room Activities Biohazardous Agents Comments Health Sciences Building, T287 EXAMPLE Cell culture of human cells, growth of lentiviral vectors, creation of transgenic plants AAV, plasmids, human cells, transgenic plant seeds, Pseudomonas aeruginosa BSL-2 tissue culture room. Certified biosafety cabinet in room. Animal Use Building/Room Activities Biohazardous Agents Comments Health Sciences Building, T287 EXAMPLE (e.g., implanting human cells in mice, perfusions of mice exposed to reovirus, housing of exposed animals) (e.g., human cell lines, murine cells transduced with gammaretroviral vectors) BSL-2 tissue culture room. Certified biosafety cabinet in room. Shared Core Facilities (e.g., MRI, FACS, hESC, UW Botany Greenhouse) Facility/Building/Room Activities Biohazardous Agents Comments MRI / Brotman B67 EXAMPLE (e.g., cell sorting, imaging of animals, flow cytometry) (e.g., cell lines, animal cells from exposed animals, cells with recombinant DNA)