(e) any from: each piece of bread three (storage conditions) place bread in different sealable plastic bags • (at) higher temperature / hotter place in different temperatures (minimum of three) • • (had) more oxygen eg fridge, room, incubator • (had) more water / moisture leave each for the same amount of time eg four • • (contained) more microorganisms (that cause days • decay) measure the percentage cover of mould on each • piece of bread reference to bacteria / fungi / repeat experiment • mould 3 additional examiner guidance: [13] good level 2 answer will describe how the growth of • mould can be measured and Q2.(a) will give a range of different temperatures to be wear a face mask used allow wear gloves allow equivalent levels of credit for alternative • 1 methodologies that would clearly produce a measurable outcome in terms of mould growth at (b) Level 2 (3–4 marks): various temperatures A detailed and coherent plan covering all the major steps. 4 It sets out the steps needed in a logical manner that could be followed by another person (c) any one from: to produce an outcome which type of mould • will address the hypothesis. amount of (put on each piece of bread) • amount of air in the plastic bags • Level 1 (1–2 marks): size of the pieces of bread • Simple statements relating to steps are made but they type of bread • may not be in a logical order. The plan may not allow amount of moisture / water added • another person to produce an outcome which will address 1 the hypothesis. (d) (56 4 = 52) / 5 − 0 1 No relevant content. 10.4 Indicative content allow 10.4 with no working shown for 2 marks Plan: 1 cut a specified number of pieces of bread to the • ecf for incorrectly read figures for same size 1 mark mould spores on the bread • the number of mould spores needs to be the same • (e) (decomposition occurs at a faster rate when the quantity of mould spores on
(e) any three from: (storage conditions) • (at) higher temperature / hotter • (had) more oxygen • (had) more water / moisture • (contained) more microorganisms (that cause decay) reference to bacteria / fungi / mould 3 [13] Q2.(a) wear a face mask allow wear gloves 1 (b) Level 2 (3–4 marks): A detailed and coherent plan covering all the major steps. It sets out the steps needed in a logical manner that could be followed by another person to produce an outcome which will address the hypothesis. Level 1 (1–2 marks): Simple statements relating to steps are made but they may not be in a logical order. The plan may not allow another person to produce an outcome which will address the hypothesis. 0 No relevant content. Indicative content Plan: • cut a specified number of pieces of bread to the same size • mould spores on the bread • the number of mould spores needs to be the same quantity of mould spores on each piece of bread • place bread in different sealable plastic bags • place in different temperatures (minimum of three) eg fridge, room, incubator • leave each for the same amount of time eg four days • measure the percentage cover of mould on each piece of bread • repeat experiment additional examiner guidance: • good level 2 answer will describe how the growth of mould can be measured and will give a range of different temperatures to be used • allow equivalent levels of credit for alternative methodologies that would clearly produce a measurable outcome in terms of mould growth at various temperatures 4 (c) any one from: • type of mould • amount of (put on each piece of bread) • amount of air in the plastic bags • size of the pieces of bread • type of bread • amount of moisture / water added 1 (d) (56 − 4 = 52) / 5 1 10.4 allow 10.4 with no working shown for 2 marks 1 ecf for incorrectly read figures for 1 mark (e) (decomposition occurs at a faster rate when the