Article type: Full Paper Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering Paola Campagnolo, Adam J Gormley, Lesley W Chow, Anne Géraldine Guex, Paresh A Parmar, Jennifer L Puetzer, Joseph AM Steele, Alexandre Breant, Paolo Madeddu, Molly M Stevens*. Dr Paola Campagnolo 1, Dr Adam J Gormley 1, Dr Lesley W Chow 1, Dr Anne Géraldine Guex 1,2 Dr Paresh A Parmar 1, Dr Jennifer L Puetzer 1, Dr Joseph AM Steele 1, Mr Alexandre Breant 1, Prof Paolo Madeddu 3, Prof Molly M Stevens 1. 1 Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, Royal School of Mines, Prince Consort Rd, SW7 2AZ, London, UK 2 National Heart and Lung Institute, Imperial College London, 435 Du Cane Road, W12 0NN, London, UK 3 Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Upper Maudlin St, BS2 8HW, Bristol, UK Keywords: tissue engineered vascular graft, electrospinning, biofunctionalization, endothelialization, pericytes Abstract The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which exposes to the risk of thrombosis, and lack of native cellular constituents, which favours pathological remodelling. Here, an bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the derived peptide (Adh) on the side and a heparin-binding peptide (Hep) on the ‘luminal’
Article type: Full Paper Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering Paola Campagnolo, Adam J Gormley, Lesley W Chow, Anne Géraldine Guex, Paresh A Parmar, Jennifer L Puetzer, Joseph AM Steele, Alexandre Breant, Paolo Madeddu, Molly M Stevens*. Dr Paola Campagnolo 1, Dr Adam J Gormley 1, Dr Lesley W Chow 1, Dr Anne Géraldine Guex 1,2 Dr Paresh A Parmar 1, Dr Jennifer L Puetzer 1, Dr Joseph AM Steele 1, Mr Alexandre Breant 1, Prof Paolo Madeddu 3, Prof Molly M Stevens 1. 1 Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, Royal School of Mines, Prince Consort Rd, SW7 2AZ, London, UK 2 National Heart and Lung Institute, Imperial College London, 435 Du Cane Road, W12 0NN, London, UK 3 Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Upper Maudlin St, BS2 8HW, Bristol, UK Keywords: tissue engineered vascular graft, electrospinning, biofunctionalization, endothelialization, pericytes Abstract The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which exposes to the risk of thrombosis, and lack of native cellular constituents, which favours pathological remodelling. Here, an bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the peptide (Adh) on the ‘luminal’ side and a heparin-binding peptide (Hep) on the derived