Analyze the set(s) of low matrix standard replicates on a single analysis day. Evaluate the precision and accuracy of the replicate matrix standard set(s) to determine LLOQ. Part 3: Evaluate the reproducibility of the IS Evaluate the IS response in the method blanks analyzed in Experiment 3, Part 1, and calculate the mean response and %RSD. Evaluate the IS response in Experiments 1 and 2 and calculate the within-day and between-day variability. ESC Experiment 2 Performed to establish the linearity, precision, and accuracy of the response; recovery of the analytes; and provide a check on the stability of the analytical system. Analyze the solvent standard calibration curve prepared in Part 2.2.1.2.2, above. Split the processed matrix standard solutions prepared in Part 2.2.1.2.3, above into two sets. Run one set of matrix standards at the beginning of the analytical run and the other set at the end. Analyze 3 method blanks run the high matrix standard. Evaluate the data for absolute recovery. Evaluate the data for instrument drift based on the split matrix curve.
Analyze the set(s) of low matrix standard replicates on a single analysis day. Evaluate the precision and accuracy of the replicate matrix standard set(s) to determine LLOQ. Part 3: Evaluate the reproducibility of the IS Evaluate the IS response in the method blanks analyzed in Experiment 3, Part 1, and calculate the mean response and %RSD. Evaluate the IS response in Experiments 1 and 2 and calculate the within-day and between-day variability. ESC Experiment 2 Performed to establish the linearity, precision, and accuracy of the response; recovery of the analytes; and provide a check on the stability of the analytical system. Analyze the solvent standard calibration curve prepared in Part 2.2.1.2.2, above. Split the processed matrix standard solutions prepared in Part 2.2.1.2.3, above into two sets. Run one set of matrix standards at the beginning of the analytical run and the other set at the end. Analyze 3 method blanks run the high matrix standard. Evaluate the data for absolute recovery. Evaluate the data for instrument drift based on the split matrix curve.