Taurocholic Acid 514.2838 C26H45NO7S 05.20 0.972362 Taurohyocholic Acid 514.2838 C26H45NO7S 03.00 0.981646 Tauro Acid 514.2838 C26H45NO7S 02.03 0.998457 α-Muricholic Acid 514.2838 C26H45NO7S 02.03 0.998457 β-Muricholic Tauro Acid 514.2838 C26H45NO7S 02.03 0.998457 ω-Muricholic Cholic Acid d4 411.3049 C24H36D4O5 14.02 NA Chenodeoxycholic Acid d4 395.3099 C24H36D4O4 19.62 NA Table S4 (continued). Preparation of bile acids for standard curve construction. Related to Experimental Procedures Concentration IS volume Solvent volume (µg/mL) (µL) (µL) Std1_1 0.64 15 132.6 Std1_2 1.28 15 130.2 Std1_3 2.56 15 125.4 Std1_4 3.76 15 120.9 Std1_5 4.96 15 116.4 Std1_6 6.24 15 111.6 Std1_7 7.52 15 106.8 Std1_8 8.72 15 102.3 Std1_9 10.00 15 97.5 Std1_10 20.00 15 60.0
Taurocholic Acid 514.2838 C26H45NO7S 05.20 0.972362 Taurohyocholic Acid 514.2838 C26H45NO7S 03.00 0.981646 Tauro α-Muricholic Acid 514.2838 C26H45NO7S 02.03 0.998457 β-Muricholic Acid 514.2838 C26H45NO7S 02.03 0.998457 Tauro ω-Muricholic Acid 514.2838 C26H45NO7S 02.03 0.998457 Cholic Acid d4 411.3049 C24H36D4O5 14.02 NA Chenodeoxycholic Acid d4 395.3099 C24H36D4O4 19.62 NA Table S4 (continued). Preparation of bile acids for standard curve construction. Related to Experimental Procedures Concentration (µg/mL) IS volume (µL) Solvent volume (µL) Std1_1 0.64 15 132.6 Std1_2 1.28 15 130.2 Std1_3 2.56 15 125.4 Std1_4 3.76 15 120.9 Std1_5 4.96 15 116.4 Std1_6 6.24 15 111.6 Std1_7 7.52 15 106.8 Std1_8 8.72 15 102.3 Std1_9 10.00 15 97.5 Std1_10 20.00 15 60.0