Standard Bottles that flag as positive on the automated system should be subcultured according to the same procedure as for blood culture bottles (see )) inoculate agar plates with specimen from blood culture bottles (see ). 2.5.3 Culture media, conditions and organisms Clinical details/ Standard media Incubation Cultures Target read organism(s) conditions Temp °C Atmosphere Time All clinical Blood culture 35 37 Air 7 d up to Continuous Any – conditions broths monitoring organism 21 d for Subculture slow positive bottles growers onto subculture + terminal plates below. subculture Subculture plates Blood agar 35 37 5 10% CO2 40 48 hr 40hr Any – – – organism Chocolate agar 35 - 37 5 10% CO2 40 - 48 hr* 40 hr Any – organism For these situations, add the following: Clinical details/ Supplementary Incubation Cultures Target conditions media read organism(s) Temp °C Atmos Time Systemic fungal Sabouraud agar 28 30 Air 5 d 3 d and 5 d Fungi – infection Microscopy Neomycin FAA 35-37 Anaerobic 7 d 2 d, 5 d and Anaerobes suggesting with 7 d anaerobes metronidazole 5 µg disc Other organisms for consideration - Mycobacterium species (see ), fungi, parasites (see ) and viruses (see www.hpa.org.uk/smi/pdf/virology). *Incubation times may be increased up to 5 days if Brucella infections are suspected 2.6 Identification 2.6.1 Minimum level of identification in the laboratory All organisms to species level. Note: Any organism considered to be a contaminant may not require identification to species level. Refer to individual SMIs for organism identification.
Standard Bottles that flag as positive on the automated system should be subcultured according to the same procedure as for blood culture bottles (see )) inoculate agar plates with specimen from blood culture bottles (see ). 2.5.3 Culture media, conditions and organisms Clinical details/ conditions Standard media Incubation Cultures read Target organism(s) Temp °C Atmosphere Time All clinical conditions Blood culture broths Subculture positive bottles onto subculture plates below. 35 – 37 Air 7 d up to 21 d for slow growers + terminal subculture Continuous monitoring Any organism Subculture plates Blood agar 35 – 37 5 – 10% CO2 40 – 48 hr 40hr Any organism Chocolate agar 35 - 37 5 – 10% CO2 40 - 48 hr* 40 hr Any organism For these situations, add the following: Clinical details/ conditions Supplementary media Incubation Cultures read Target organism(s) Temp °C Atmos Time Systemic fungal infection Sabouraud agar 28 – 30 Air 5 d 3 d and 5 d Fungi Microscopy suggesting anaerobes Neomycin FAA with metronidazole 5 µg disc 35-37 Anaerobic 7 d 2 d, 5 d and 7 d Anaerobes Other organisms for consideration - Mycobacterium species (see ), fungi, parasites (see ) and viruses (see www.hpa.org.uk/smi/pdf/virology). *Incubation times may be increased up to 5 days if Brucella infections are suspected 2.6 Identification 2.6.1 Minimum level of identification in the laboratory All organisms to species level. Note: Any organism considered to be a contaminant may not require identification to species level. Refer to individual SMIs for organism identification.