Figure 4.6 Agarose gel electrophoresis (1.5%) for end point PCR to visualise 169 GAPDH cDNA expression in control virus cells, water control and RAMP3 knock down cells for two weeks out of selection. Figure 4.7 1.5% Agarose gel electrophoresis for end point PCR to visualise 170 RAMP3 cDNA expression in RAMP3 knock down cells out of selection for 5 weeks. Figure 4.8 1.5 % Agarose gel electrophoresis for end point PCR to detect 171 the expression of GAPDH cDNA in control virus cells, water control and RAMP3 knock down cells Figure 4.9 Agarose gel electrophoresis for end point PCR to visualise the 173 expression of CLR, AM and LOXL-2 cDNA in the positive control (mouse brain), RAMP3 knock down cells and the water control. Figure 4.10 Agarose gel electrophoresis for end point PCR to detect the 174 GAPDH cDNA expression in positive control (mouse brain), RAMP3 knock down cells and water control. Figure 4.11 Agarose gel electrophoresis for end PCR indicates the 176 expression of the PTH1R, PTH2R and GAPDH cDNA in RAMP3 knock down cells, positive control and water control. Figure 4.12 Agarose gel electrophoresis (1.5%) for end PCR to visualise 176 CTR, VIPR-1 and GAPDH cDNA in RAMP3 knock down cells, positive control (mouse brain) and water control. Figure 4.13 Agarose gel electrophoresis for end point PCR indicates the 180 expression of the CaSR, Glucagon and GAPDH cDNA in RAMP3 knock down cells, positive control and water control. Figure 4.14 Agarose gel electrophoresis for end point PCR to visualise the 182 expression of SCTR and GAPDH cDNA in RAMP3 knock down cells and the positive control (mouse pancreas), water control. Figure 4.15 A graphical demonstration of relative cDNA expression of the 184 reference genes Figure 4.16 Graphical demonstration of average expression stability of 185 geNorm value of the reference genes in the control cells “M” and RAMP3 knockdown cells.
Figure 4.6 Agarose gel electrophoresis (1.5%) for end point PCR to visualise GAPDH cDNA expression in control virus cells, water control and RAMP3 knock down cells for two weeks out of selection. 169 Figure 4.7 1.5% Agarose gel electrophoresis for end point PCR to visualise RAMP3 cDNA expression in RAMP3 knock down cells out of selection for 5 weeks. 170 Figure 4.8 1.5 % Agarose gel electrophoresis for end point PCR to detect the expression of GAPDH cDNA in control virus cells, water control and RAMP3 knock down cells 171 Figure 4.9 Agarose gel electrophoresis for end point PCR to visualise the expression of CLR, AM and LOXL-2 cDNA in the positive control (mouse brain), RAMP3 knock down cells and the water control. 173 Figure 4.10 Agarose gel electrophoresis for end point PCR to detect the GAPDH cDNA expression in positive control (mouse brain), RAMP3 knock down cells and water control. 174 Figure 4.11 Agarose gel electrophoresis for end PCR indicates the expression of the PTH1R, PTH2R and GAPDH cDNA in RAMP3 knock down cells, positive control and water control. 176 Figure 4.12 Agarose gel electrophoresis (1.5%) for end PCR to visualise CTR, VIPR-1 and GAPDH cDNA in RAMP3 knock down cells, positive control (mouse brain) and water control. 176 Figure 4.13 Agarose gel electrophoresis for end point PCR indicates the expression of the CaSR, Glucagon and GAPDH cDNA in RAMP3 knock down cells, positive control and water control. 180 Figure 4.14 Agarose gel electrophoresis for end point PCR to visualise the expression of SCTR and GAPDH cDNA in RAMP3 knock down cells and the positive control (mouse pancreas), water control. 182 Figure 4.15 A graphical demonstration of relative cDNA expression of the reference genes 184 Figure 4.16 Graphical demonstration of average expression stability of geNorm “M” value of the reference genes in the control cells and RAMP3 knockdown cells. 185