Required practical Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown sample ‘urine’ Apparatus and b. Use appropriate instrumentation to record quantitative techniques covered measurements, such as a colorimeter c. Use laboratory glassware apparatus for a variety of experimental (Not full statements) techniques to include serial dilutions f. Use qualitative reagents to identify biological molecules Indicative apparatus glucose solution, distilled water, Clinistix, Benedicts solution, graduated pipettes and fillers, test tubes, water bath, colorimeter and cuvettes Amount of choice Increasing independence Least choice Some choice Many choices Full investigation Teacher Teacher Student decides Student is presented chooses the provides on range of with the urine of concentration of concentration glucose suspected diabetic. standard of standard concentrations Student researches glucose solution glucose for calibration methods for finding out and values for solution but curve. the concentration of serial dilution. students Students glucose in the urine, Volumes of decide on produce then chooses glucose solution values for dilutions and equipment and and water are serial dilution. after reacting materials, justifying all given for serial Students with Benedicts choices. dilution. produce reagent produce Students dilutions and a calibration produce after reacting curve. Unknown dilutions and with Benedicts sample is ‘urine’ after reacting reagent reacted with with Benedicts produce a Benedicts reagent produce calibration reagent and the a calibration curve. value of glucose curve. Unknown concentration is Unknown sample read from the ‘urine’ ‘urine’ sample is is reacted with calibration reacted with Benedicts curve. Benedicts reagent and Teacher reagent and the the value of provides outline value of glucose glucose method only. concentration is concentration read from the is read from calibration the calibration curve. curve. Experiments Experiment fully specified in probably fully terms of specified by equipment and teacher. method.
Required practical Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown ‘urine’ sample Apparatus and techniques covered (Not full statements) b. Use appropriate instrumentation to record quantitative measurements, such as a colorimeter c. Use laboratory glassware apparatus for a variety of experimental techniques to include serial dilutions f. Use qualitative reagents to identify biological molecules Indicative apparatus glucose solution, distilled water, Clinistix, Benedicts solution, graduated pipettes and fillers, test tubes, water bath, colorimeter and cuvettes Amount of choice Increasing independence Least choice Some choice Many choices Full investigation Teacher chooses the concentration of standard glucose solution and values for serial dilution. Volumes of glucose solution and water are given for serial dilution. Students produce dilutions and after reacting with Benedicts reagent produce a calibration curve. Unknown ‘urine’ sample is reacted with Benedicts reagent and the value of glucose concentration is read from the calibration curve. Experiments fully specified in terms of equipment and method. Teacher provides concentration of standard glucose solution but students decide on values for serial dilution. Students produce dilutions and after reacting with Benedicts reagent produce a calibration curve. Unknown ‘urine’ sample is reacted with Benedicts reagent and the value of glucose concentration is read from the calibration curve. Experiment probably fully specified by teacher. Student decides on range of glucose concentrations for calibration curve. Students produce dilutions and after reacting with Benedicts reagent produce a calibration curve. Unknown ‘urine’ sample is reacted with Benedicts reagent and the value of glucose concentration is read from the calibration curve. Teacher provides outline method only. Student is presented with the urine of suspected diabetic. Student researches methods for finding out the concentration of glucose in the urine, then chooses equipment and materials, justifying all choices.