In all activities, replace the cap on the bottles and touch the tip of the bottle to the NOTE: DO NOT GLASS PLATE or to the solution in the position. This could contaminate the reagents for the remainder of the students. When placing the droplets, hold the bottle vertically and squeeze gently, dropping only one drop per well. Activity 1 Determining the effect of pH on indicator dyes – Purpose: To determine the color change for each of 3 indicators: methyl orange, bromo thymol blue and phenolphthalein. Procedure: Place the GLASS PLATE on a sheet of white paper. Place 1 drop of methyl orange in position #1 and #2. 1 drop of brom thymol blue in position #3 and #4. Place 1 drop of phenylphthalein in position #5 and #6. Carefully add 1 drop of pH 1 to position #1, #3 and #5. Carefully add 1 drop of pH 13 to position #2, #4 and #6. Record your observations on your data for this exercise. Rinse the GLASS PLATE with tap water in the sink and dry with a paper towel. RESULTS: Activity 1– Determining the effect of pH on indicator dyes
NOTE: In all activities, replace the cap on the bottles and DO NOT touch the tip of the bottle to the GLASS PLATE or to the solution in the position. This could contaminate the reagents for the remainder of the students. When placing the droplets, hold the bottle vertically and squeeze gently, dropping only one drop per well. Activity 1 – Determining the effect of pH on indicator dyes Purpose: To determine the color change for each of 3 indicators: methyl orange, bromo thymol blue and phenolphthalein. Procedure: Place the GLASS PLATE on a sheet of white paper. Place 1 drop of methyl orange in position #1 and #2. 1 drop of brom thymol blue in position #3 and #4. Place 1 drop of phenylphthalein in position #5 and #6. Carefully add 1 drop of pH 1 to position #1, #3 and #5. Carefully add 1 drop of pH 13 to position #2, #4 and #6. Record your observations on your data for this exercise. Rinse the GLASS PLATE with tap water in the sink and dry with a paper towel. RESULTS: Activity 1– Determining the effect of pH on indicator dyes