Save the Skyline file as in folder. PRM_ttest.sky Skyline Webinar Export a custom-defined report Skyline allows you to export from the Skyline document to a .csv file many values and statistics that can be used for further processing in other tools like excel or R. The Skyline Results and Document Grids provide access to many of these values and allow you to edit custom annotations as you work with your data. We saw an example of custom report before and now we will generate a report with some quantitative data to further illustrate how to use this Skyline feature. To generate the report do the following: “File” “Export” “Report” The tab offers you a range of predefined report formats. Here we will “Report” generate our own report format: “Edit list” “Add...” An window opens. Note: For detailed information about all options “Edit Report” see the Skyline tutorial and Results on the “Skyline Custom Reports Grids” Skyline website. Name the report From the selection tree on the left, select the “PRM-Quant”. following items: Name” “Protein (“Proteins” Name”) → “Protein Modified Sequence” “Peptide (“Proteins” → “Peptides” → “Peptide Modified Sequence”) area ratio of light to heavy; sum of all “RatioToStandard” (Peptide transition areas) ” (“Proteins” Results” → “Peptides” → “Peptide → “Ratio To Standard”) Concentration” “Calculated (“Proteins” → “Peptides” → “Peptide Results” Concentration”) → “Quantification” → “Calculated Add a check mark to in the bottom of the window. “Pivot Replicate Name” Your Edit Report tab should now look like this: Click and save the report as a spreadsheet file in the folder with “OK” Webinar the name PRM-Quant.csv Save your Skyline session. Bibliography from the Skyline website. “Skyline Processing Grouped Study Data” Tutorial Custom and Tutorial from the Skyline website. “Skyline Live Reports”
Save the Skyline file as PRM_ttest.sky in Skyline Webinar folder. Export a custom-defined report Skyline allows you to export from the Skyline document to a .csv file many values and statistics that can be used for further processing in other tools like excel or R. The Skyline Results and Document Grids provide access to many of these values and allow you to edit custom annotations as you work with your data. We saw an example of custom report before and now we will generate a report with some quantitative data to further illustrate how to use this Skyline feature. To generate the report do the following: “File” “Export” “Report” The “Report” tab offers you a range of predefined report formats. Here we will generate our own report format: “Edit list” “Add...” An “Edit Report” window opens. Note: For detailed information about all options see the Skyline tutorial “Skyline Custom Reports and Results Grids” on the Skyline website. Name the report “PRM-Quant”. From the selection tree on the left, select the following items: “Protein Name” (“Proteins” → “Protein Name”) “Peptide Modified Sequence” (“Proteins” → “Peptides” → “Peptide Modified Sequence”) “RatioToStandard” (Peptide area ratio of light to heavy; sum of all transition areas) ” (“Proteins” → “Peptides” → “Peptide Results” → “Ratio To Standard”) “Calculated Concentration” (“Proteins” → “Peptides” → “Peptide Results” → “Quantification” → “Calculated Concentration”) Add a check mark to “Pivot Replicate Name” in the bottom of the window. Your Edit Report tab should now look like this: Click “OK” and save the report as a spreadsheet file in the Webinar folder with the name PRM-Quant.csv Save your Skyline session. Bibliography “Skyline Processing Grouped Study Data” Tutorial from the Skyline website. “Skyline Custom and Live Reports” Tutorial from the Skyline website.