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Focusing on pH. Students may complete Assignment 2: Investigating the effect of pH on Enzymes. Students may perform a practical investigating the effect of pH on Pepsin. • The effects of the following factors on the rate of enzyme controlled reactions – enzyme concentration, substrate concentration, concentration of competitive and of inhibitors, pH and temperature. MS 0.5 Students could be given the hydrogen ion concentration of a solution in order to calculate its pH, using the formula: pH = −log10[H+] 8 Factors effecting Enzyme function Focusing on competitive and inhibition. Students could investigate how lead inhibits catechol oxidase. Describe the effect of competitive and inhibition on enzyme action 3.1.4.2 Many Proteins are Enzymes • The effects of the following factors on the rate of enzyme controlled reactions – enzyme concentration, substrate concentration, concentration of competitive and of inhibitors, pH and temperature. 3.4 9 Factors effecting enzyme function Students can complete Assignment 3: Investigating enzymes for the future and research enzyme use further with access to the internet. Describe the factors which affect the rate of enzyme action 3.1.4.2 Many Proteins are Enzymes • The effects of the following factors on the rate of enzyme controlled reactions – enzyme concentration, substrate concentration, concentration of competitive and of inhibitors, pH and temperature. MS0.1 MS0.3 PS1.1 PS1.2 3.4 10 Factors effecting enzyme function Students can complete Assignment 4: Measuring the activity of amylase. This can also be adapted as a practical task. Describe the factors which affect the rate of enzyme action 3.1.4.2 Many Proteins are Enzymes • The effects of the following factors on the rate of enzyme controlled reactions – enzyme concentration, substrate concentration, concentration of MS1.3 MS3.2 MS4.1 PS1.1 PS2.3 PS3.1 PS3.2 3.4