P20 and tips PART A: BASIC ABSORBANCE, Loading slide TRANSMITTANCE, using trypan AND blue stain Combine 10 µl CONCENTRATION cell suspension Turn on the with 10 µl of TC10 trypan blue dye Automated in a Cell Counter microcentrifuge via the green tube. Pipet square button gently up and on side down to mix. bottom of equipment. 10 µl of the mixture into a Wait for the TC10 sample instrument to slide. Place initialize. pipet tip at 45 degree angle Prepare slide at the bottom to be of sample analyzed loading area while waiting. (the half-circle at outer end of Select options the chamber). on the home Only load into screen by the outer end of pressing the the counting [UP] or slide. The [DOWN] key center notches on the are for navigation distribution key pad. purposes. When your selection is highlighted in green, press [ENTER] on the navigation key pad. Choose Count Cells and insert the The cell prepared counter
P20 and tips PART A: BASIC ABSORBANCE, TRANSMITTANCE, AND CONCENTRATION Turn on the TC10 Automated Cell Counter via the green square button on side bottom of equipment. Wait for the instrument to initialize. Prepare slide to be analyzed while waiting. Select options on the home screen by pressing the [UP] or [DOWN] key on the navigation key pad. When your selection is highlighted in green, press [ENTER] on the navigation key pad. Choose Count Cells and insert the prepared Loading slide using trypan blue stain Combine 10 µl cell suspension with 10 µl of trypan blue dye in a microcentrifuge tube. Pipet gently up and down to mix. 10 µl of the mixture into a TC10 sample slide. Place pipet tip at 45 degree angle at the bottom of sample loading area (the half-circle at outer end of the chamber). Only load into the outer end of the counting slide. The center notches are for distribution purposes. The cell counter