DV: Growing or Glowing; RESULTS- Growth on no plasmid without ampicllin, no growth with no plasmid amp; growth on plasmid amp and no amp but glowed on amp Equations: Transformation Efficiency- total number of colonies growing on the agar plate/ amount of DNA spread on the LB /amp plate in ug AP Biology Investigation 9: Biotechnology- Restriction Enzyme Analysis of DNA Overview: A sample electrophoresis experiment was performed regarding cancer in patients. A patient was tested to see if the DNA from her breast, blood, surrounding tissue and a control group. IV: Sample location DV: RFLP’s or banding pattern on electrophoresis gel; one hit = carrier; two hit= cancer Equations: This lab was a substitution lab the original AP lab asked students to make a standard curve (we did this in class as an activity) by electrophoresing known fragment lengths and then measuring the distance they traveled to create a standard curve. This curve was used to estimate the sizes of UNKNOWN sized fragments in other lanes. -------------------------------------------------------------------------------------------------------------------------------------------------- Molecular Genetics conjugation HOX genes reverse transcriptase DNA Sequencing Human genome project RNAi DNA ligase inducible genes small interfering RNA (siRNA) DNA polymerase lac operon small regulatory RNA gel electrophoresis RNA (miRNA) transgenic organism gene expression plasmid transposon gene induction polymerase chain reaction transformation gene repression probe vector genetic engineering regulatory sequence virus homeotic genes restriction enzyme and Practice What are some mechanisms by which gene expression is regulated in prokaryotes and eukaryotes? What is the structure of viruses? How do viruses transfer genetic material between cells? What are some current recombination technologies? What are some practical applications of nucleic acid technology?
DV: Growing or Glowing; RESULTS- Growth on no plasmid without ampicllin, no growth with no plasmid amp; growth on plasmid amp and no amp but glowed on amp Equations: Transformation Efficiency- total number of colonies growing on the agar plate/ amount of DNA spread on the LB /amp plate in ug AP Biology Investigation 9: Biotechnology- Restriction Enzyme Analysis of DNA Overview: A sample electrophoresis experiment was performed regarding cancer in patients. A patient was tested to see if the DNA from her breast, blood, surrounding tissue and a control group. IV: Sample location DV: RFLP’s or banding pattern on electrophoresis gel; one hit = carrier; two hit= cancer Equations: This lab was a substitution lab the original AP lab asked students to make a standard curve (we did this in class as an activity) by electrophoresing known fragment lengths and then measuring the distance they traveled to create a standard curve. This curve was used to estimate the sizes of UNKNOWN sized fragments in other lanes. -------------------------------------------------------------------------------------------------------------------------------------------------- Molecular Genetics conjugation DNA Sequencing DNA ligase DNA polymerase gel electrophoresis gene expression gene induction gene repression genetic engineering homeotic genes HOX genes Human genome project inducible genes lac operon RNA (miRNA) plasmid polymerase chain reaction probe regulatory sequence restriction enzyme reverse transcriptase RNAi small interfering RNA (siRNA) small regulatory RNA transgenic organism transposon transformation vector virus and Practice What are some mechanisms by which gene expression is regulated in prokaryotes and eukaryotes? What is the structure of viruses? How do viruses transfer genetic material between cells? What are some current recombination technologies? What are some practical applications of nucleic acid technology?