The notified chemical was not found to be mutagenic in bacteria and did not induce an increase in micronuclei in the mouse micronucleus assay. No clastogenic effects were in vivo found in the Chinese hamster ovary cell cytogenic assay. in vitro Based on the data provided, the notified chemical would not be classified as hazardous according to the NOHSC (National Approved Criteria for Classifying Hazardous Substances Occupational Health and Safety Commission, 1994a). ASSESSMENT OF ENVIRONMENTAL EFFECTS The following ecotoxicity studies have been supplied by the notifier. The tests were carried out according to OECD Test Methods. (Organisation for Economic Cooperation and Development, 1984; Organisation for Economic Cooperation and Development, 1992b). Test Species Results (Nominal Concentrations Reference of WAFa) LL50b > 1,000 mg/L (Febbo, 1997a) acute toxicity rainbow trout EL50c > 1,000 mg/L (Febbo, 1997b) acute toxicity Daphnia magna accommodated fraction - see text below; Lethal Loading; Effect Loading aWater bLL50: cEL50: Due to the low water solubility of the notified substance, the studies were performed to determine the toxicity of the water accommodated fraction (WAF). A 1 000 mg/L treatment was prepared and stirred for 24 hours. After settling for 1 hour, the WAF was removed and used as the treatment solution. The WAFs were slightly cloudy. The notified substance can be classed as non-toxic to rainbow trout and water fleas, up to its limit of solubility (the WAF). No study on the toxicity of the notified substance to algae has been submitted by the notifier. The notifier justifies this omission on the grounds that the substance exhibits low toxicity to other aquatic organisms and mammals. This is acceptable as a component of the notified substance for which the 1,000 mg/L WAF was not toxic to algae has been previously assessed. ASSESSMENT OF ENVIRONMENTAL HAZARD Environmental releases of the material are expected to be low as both product formulation and crankcase filling are performed under well controlled conditions. Spills and other losses will be minimal (<2% of imported chemical). In excess of 98 percent of used oil containing the notified substance will be disposed of
The notified chemical was not found to be mutagenic in bacteria and did not induce an increase in micronuclei in the in vivo mouse micronucleus assay. No clastogenic effects were found in the in vitro Chinese hamster ovary cell cytogenic assay. Based on the data provided, the notified chemical would not be classified as hazardous according to the NOHSC Approved Criteria for Classifying Hazardous Substances (National Occupational Health and Safety Commission, 1994a). ASSESSMENT OF ENVIRONMENTAL EFFECTS The following ecotoxicity studies have been supplied by the notifier. The tests were carried out according to OECD Test Methods. (Organisation for Economic Cooperation and Development, 1984; Organisation for Economic Cooperation and Development, 1992b). Test Species Results (Nominal Concentrations of WAFa) Reference acute toxicity rainbow trout LL50b > 1,000 mg/L (Febbo, 1997a) acute toxicity Daphnia magna EL50c > 1,000 mg/L (Febbo, 1997b) aWater accommodated fraction - see text below; bLL50: Lethal Loading; cEL50: Effect Loading Due to the low water solubility of the notified substance, the studies were performed to determine the toxicity of the water accommodated fraction (WAF). A 1 000 mg/L treatment was prepared and stirred for 24 hours. After settling for 1 hour, the WAF was removed and used as the treatment solution. The WAFs were slightly cloudy. The notified substance can be classed as non-toxic to rainbow trout and water fleas, up to its limit of solubility (the WAF). No study on the toxicity of the notified substance to algae has been submitted by the notifier. The notifier justifies this omission on the grounds that the substance exhibits low toxicity to other aquatic organisms and mammals. This is acceptable as a component of the notified substance for which the 1,000 mg/L WAF was not toxic to algae has been previously assessed. ASSESSMENT OF ENVIRONMENTAL HAZARD Environmental releases of the material are expected to be low as both product formulation and crankcase filling are performed under well controlled conditions. Spills and other losses will be minimal (<2% of imported chemical). In excess of 98 percent of used oil containing the notified substance will be disposed of