Table S1. Mycobacterial strains and plasmids used in this study Strain Descriptions Ref. PM759 [] Mycobacterium smegmatis ∆lys∆blaS JM578 [] Mycobacterium smegmatis ∆lys∆blaS∆tatA JM576 [] Mycobacterium smegmatis ΔtatC mc2155 [] Mycobacterium smegmatis wild type mc24517 mc2155 strain harbouring T7 RNA polymerase gene [] Mycobacterium smegmatis PM638 [] Mycobacterium tuberculosis ∆blaC H37Rv Mycobacterium tuberculosis – H37Ra Mycobacterium tuberculosis – Plasmid Multicopy plasmid with hsp60 promoter, mycobacterial origin of replication and pMV261 [] kanamycin resistance cassette pMP327 BlaC in pMV261 [] pJM106 BlaC in pCC (Epicenter) [] M. tuberculosis mature domain (‘BlaC) pJM111 PlcB signal sequence fused in frame with the mature domain of BlaC in pMV261 [] pJM113 The mature domain of BlaC (no signal sequence) in pMV261 [] [] pJSC77 pMV261 with HA tag downstream of hsp60 promoter pEP106 Cloning intermediate Rv0132c signal sequence in pCR2.1 This study pEP107 Cloning intermediate Rv0132c signal sequence in pMV261 This study pEP108 Rv0132c signal sequence fused in frame with the mature domain of BlaC This study Multicopy shuttle vector with T7 promoter, and mycobacterial origins of E. coli pYUB28b [] replication and hygromycin resistance cassette Destination vector for Gateway cloning system, multicopy shuttle vector with T7 pDESTsmg [] promoter, and mycobacterial origins of replication and hygromycin resistance E. coli Rv0132c–smg Rv0132c–Δ38 (no signal sequence) cloned into pDESTsmg This study Rv0132c-HA Rv0132c cloned into pJSC77 (HA tag in frame) This study
Table S1. Mycobacterial strains and plasmids used in this study Strain Descriptions Ref. PM759 Mycobacterium smegmatis ∆lys∆blaS [] JM578 Mycobacterium smegmatis ∆lys∆blaS∆tatA [] JM576 Mycobacterium smegmatis ΔtatC [] mc2155 Mycobacterium smegmatis wild type [] mc24517 Mycobacterium smegmatis mc2155 strain harbouring T7 RNA polymerase gene [] PM638 Mycobacterium tuberculosis ∆blaC [] H37Rv Mycobacterium tuberculosis – H37Ra Mycobacterium tuberculosis – Plasmid pMV261 Multicopy plasmid with hsp60 promoter, mycobacterial origin of replication and kanamycin resistance cassette [] pMP327 BlaC in pMV261 [] pJM106 M. tuberculosis BlaC mature domain (‘BlaC) in pCC (Epicenter) [] pJM111 PlcB signal sequence fused in frame with the mature domain of BlaC in pMV261 [] pJM113 The mature domain of BlaC (no signal sequence) in pMV261 [] pJSC77 pMV261 with HA tag downstream of hsp60 promoter [] pEP106 Cloning intermediate Rv0132c signal sequence in pCR2.1 This study pEP107 Cloning intermediate Rv0132c signal sequence in pMV261 This study pEP108 Rv0132c signal sequence fused in frame with the mature domain of BlaC This study pYUB28b Multicopy shuttle vector with T7 promoter, E. coli and mycobacterial origins of replication and hygromycin resistance cassette [] pDESTsmg Destination vector for Gateway cloning system, multicopy shuttle vector with T7 promoter, E. coli and mycobacterial origins of replication and hygromycin resistance [] Rv0132c–smg Rv0132c–Δ38 (no signal sequence) cloned into pDESTsmg This study Rv0132c-HA Rv0132c cloned into pJSC77 (HA tag in frame) This study