Day 1 Day 2 Day 3 Day 4 FC FC FC FC ∆Ct ∆Ct ∆Ct ∆Ct Scrambled 10.49 10.74 11.66 11.39 Pre-miR -5.97 90576 -6.01 115756 -5.45 141192 -3.83 38086 Anti-miR 10.87 0.77 11.68 0.52 10.65 2.00 11.31 1.05 Table 24: Table showing of microRNA-1224-3p with respect to the ∆Ct reference gene RNU 48 using Lipofectamine® RNA iMAX as the transfection agent. A significant over-expression of microRNA-1224-3p occurred throughout all 4 days of incubation with its optimal effect on day 3. No reliable fold-changes (FC) were seen on the under-expression of miR-1224-3p throughout the 4 days of incubation. Figure 17: The of mir-1224-3p in comparison to the reference gene RNU 48 ∆Ct for days 1 to 4 with an anti-miR-1224-3p, pre-miR-1224-3p, a scrambled sequence and a mock transfection Lipofectamine® RNA iMAX. The ∆Ct values of the transfections with a scrambled sequence and anti-miR-1224 was of a similar value. 5.2.2 MTT assay for microRNA-1224-3p over-expression on NHU cells. The transfection and over-expression of mir-1224-3p had an inhibitory effect on the cellular activity of NHU cells. This was of a reproducible nature as the results were confirmed when repeating the experiment (table 25).
Day 1 Day 2 Day 3 Day 4 ∆Ct FC ∆Ct FC ∆Ct FC ∆Ct FC Scrambled 10.49 10.74 11.66 11.39 Pre-miR -5.97 90576 -6.01 115756 -5.45 141192 -3.83 38086 Anti-miR 10.87 0.77 11.68 0.52 10.65 2.00 11.31 1.05 Table 24: Table showing ∆Ct of microRNA-1224-3p with respect to the reference gene RNU 48 using Lipofectamine® RNA iMAX as the transfection agent. A significant over-expression of microRNA-1224-3p occurred throughout all 4 days of incubation with its optimal effect on day 3. No reliable fold-changes (FC) were seen on the under-expression of miR-1224-3p throughout the 4 days of incubation. Figure 17: The ∆Ct of mir-1224-3p in comparison to the reference gene RNU 48 for days 1 to 4 with an anti-miR-1224-3p, pre-miR-1224-3p, a scrambled sequence and a mock transfection Lipofectamine® RNA iMAX. The ∆Ct values of the transfections with a scrambled sequence and anti-miR-1224 was of a similar value. 5.2.2 MTT assay for microRNA-1224-3p over-expression on NHU cells. The transfection and over-expression of mir-1224-3p had an inhibitory effect on the cellular activity of NHU cells. This was of a reproducible nature as the results were confirmed when repeating the experiment (table 25).