Using the Microscope (STEM 1.0) Benchmarks: The proper use of the microscope will enable students to study some of the standards covered in the NGSSS Life Science Body of Knowledge. Investigate and explain the components of the scientific theory of cells (cell theory): SC.6.L.14.2 all organisms are composed of cells (single-celled or multi-cellular), all cells come from cells, and cells are the basic unit of life. (AA) Objectives/Purpose: correctly prepare a wet-mount slide observe an object under the microscope properly use a scientific tool used to observe cells In the science laboratory, the microscope is used to examine Background Information: organisms and objects that are too small to be seen with the unaided eye. The objects to be viewed are placed on a glass slide. The glass slide may be either a dry-mount or a wet-mount slide. In a dry-mount slide, the object to be examined is placed on the slide and covered with a cover slip. In a wet-mount slide, a drop of the liquid containing the object to be examined is placed on the slide and then covered with a cover slip. Problem: How do you prepare an object to be viewed under the microscope? How do you see the microscope to observe an object? Materials: Microscope Medicine dropper Microscope slide Water Cover slip Forceps or pin Newspaper Scissors Procedures: Cut a small from the newspaper and place it in the center of a clean microscope letter “d” slide so that it is in the normal reading position. For you to observe any specimen Note: with a compound microscope, the specimen must be thin enough for light to pass through it. To make a wet-mount slide, use the medicine dropper to carefully place a small drop of water over the specimen to be observed. (letter “d”) Place one side of a clean cover slip at the edge of the drop of water at a angle. Using 45ọ forceps or a pin, carefully lower the cover slip over the and the drop of water. Try letter “d” not to trap air bubbles under the cover slip, since these will interfere with your view of the specimen. Now you have a wet-mount slide. In Figure 2, draw a picture of the as you see it on the slide. letter “d”
Using the Microscope (STEM 1.0) Benchmarks: The proper use of the microscope will enable students to study some of the standards covered in the NGSSS Life Science Body of Knowledge. SC.6.L.14.2 Investigate and explain the components of the scientific theory of cells (cell theory): all organisms are composed of cells (single-celled or multi-cellular), all cells come from cells, and cells are the basic unit of life. (AA) Objectives/Purpose: correctly prepare a wet-mount slide observe an object under the microscope properly use a scientific tool used to observe cells Background Information: In the science laboratory, the microscope is used to examine organisms and objects that are too small to be seen with the unaided eye. The objects to be viewed are placed on a glass slide. The glass slide may be either a dry-mount or a wet-mount slide. In a dry-mount slide, the object to be examined is placed on the slide and covered with a cover slip. In a wet-mount slide, a drop of the liquid containing the object to be examined is placed on the slide and then covered with a cover slip. Problem: How do you prepare an object to be viewed under the microscope? How do you see the microscope to observe an object? Materials: Microscope Microscope slide Cover slip Newspaper Medicine dropper Water Forceps or pin Scissors Procedures: Cut a small letter “d” from the newspaper and place it in the center of a clean microscope slide so that it is in the normal reading position. Note: For you to observe any specimen with a compound microscope, the specimen must be thin enough for light to pass through it. To make a wet-mount slide, use the medicine dropper to carefully place a small drop of water over the specimen (letter “d”) to be observed. Place one side of a clean cover slip at the edge of the drop of water at a 45ọ angle. Using forceps or a pin, carefully lower the cover slip over the letter “d” and the drop of water. Try not to trap air bubbles under the cover slip, since these will interfere with your view of the specimen. Now you have a wet-mount slide. In Figure 2, draw a picture of the letter “d” as you see it on the slide.