Ocular secretion occurred in one animal one hour post Comment: application. Control eyes showed no abnormal clinical signs. Conjunctival redness and chemosis was observed in all animals. Conjunctival signs persisted for up to 14 days. Corneal opacity in one animal failed to resolve after 21 days. The neat notified chemical caused severe damage to the eyes Result: of rabbits. 9.1.6 Eye Irritation (5% Solution) (Kaufmann, 1992) Rabbits, New Zealand White Species/strain: 3, sex not specified Number/sex of animals: 21 days Observation period: 0.1 mL of a 5% solution of test article in sterile water Method of administration: introduced into the conjunctival sac of the left eye; control right eyes remained untreated. OECD TG 405 Test method: Conjunctival redness (score 11) was noted in all three Comment: animals one hour after administration. Conjunctival irritation was resolved completely within 24 hours. No other signs of irritation to the cornea, iris or conjunctiva were noted. The 5% solution of notified chemical was mildly irritating to Result: the eyes of rabbits. 1 see Attachment 1 for Draize scales 9.1.7 Skin Sensitisation (Buchholz, 1991) Guinea pig, Pirbright White Species/strain: Test: 10 males, 10 females Number of animals: Control: 10 males, 10 females Intradermal injections followed by dermal application Induction procedure: test group: Three pairs of 0.1mL intradermal injections into the shaved day 0 neck region:
Comment: Ocular secretion occurred in one animal one hour post application. Control eyes showed no abnormal clinical signs. Conjunctival redness and chemosis was observed in all animals. Conjunctival signs persisted for up to 14 days. Corneal opacity in one animal failed to resolve after 21 days. Result: The neat notified chemical caused severe damage to the eyes of rabbits. 9.1.6 Eye Irritation (5% Solution) (Kaufmann, 1992) Species/strain: Rabbits, New Zealand White Number/sex of animals: 3, sex not specified Observation period: 21 days Method of administration: 0.1 mL of a 5% solution of test article in sterile water introduced into the conjunctival sac of the left eye; control right eyes remained untreated. Test method: OECD TG 405 Comment: Conjunctival redness (score 11) was noted in all three animals one hour after administration. Conjunctival irritation was resolved completely within 24 hours. No other signs of irritation to the cornea, iris or conjunctiva were noted. Result: The 5% solution of notified chemical was mildly irritating to the eyes of rabbits. 1 see Attachment 1 for Draize scales 9.1.7 Skin Sensitisation (Buchholz, 1991) Species/strain: Guinea pig, Pirbright White Number of animals: Test: 10 males, 10 females Control: 10 males, 10 females Induction procedure: Intradermal injections followed by dermal application test group: day 0 Three pairs of 0.1mL intradermal injections into the shaved neck region: