tubing modification 2 0.3 1.79 tubing modification 3 0.3 3.07 tubing modification 4 0.6 0.50 tubing modification 5 0.6 1.79 tubing modification 6 0.6 3.07 tubing modification 7 0.9 0.50 tubing modification 8 0.9 1.79 tubing modification 9 0.9 3.07 Table S-2. Design of experiments for the investigation of 4 parameters (crimping depth, distance between and flow rate) using a full factorial design. The stationary crimping, β-value phase retention was measured in a hydrophobic (HAcn) and an medium (HTWM) polarity Sf solvent system. HAcna HTMWb Solvent system (HT) (TH) (HT) (TH) Sf Sf Sf Sf No. crimping depth distance between crimping ß value flow rate [mm] [cm] [mL/min] 0.86 0.77 0.81 1 0.3 3.07 0.54 0.5 0.87 0.85 0.85 0.84 2 0.9 0.50 0.62 0.5 0.86 0.84 0.76 0.81 3 0.6 3.07 0.62 1.0 0.86 0.81 0.71 0.74 4 0.6 0.50 0.58 1.0 0.83 0.81 0.71 0.74 5 0.9 3.07 0.54 1.0 0.83 0.80 0.78 0.83 6 0.9 1.79 0.62 2.0 0.83
tubing modification 2 0.3 1.79 tubing modification 3 0.3 3.07 tubing modification 4 0.6 0.50 tubing modification 5 0.6 1.79 tubing modification 6 0.6 3.07 tubing modification 7 0.9 0.50 tubing modification 8 0.9 1.79 tubing modification 9 0.9 3.07 Table S-2. Design of experiments for the investigation of 4 parameters (crimping depth, distance between crimping, β-value and flow rate) using a full factorial design. The stationary phase retention Sf was measured in a hydrophobic (HAcn) and an medium (HTWM) polarity solvent system. Solvent system HAcna HTMWb No. crimping depth [mm] distance between crimping [cm] ß value flow rate [mL/min] Sf (HT) Sf (TH) Sf (HT) Sf (TH) 1 0.3 3.07 0.54 0.5 0.86 0.87 0.77 0.81 2 0.9 0.50 0.62 0.5 0.85 0.86 0.85 0.84 3 0.6 3.07 0.62 1.0 0.84 0.86 0.76 0.81 4 0.6 0.50 0.58 1.0 0.81 0.83 0.71 0.74 5 0.9 3.07 0.54 1.0 0.81 0.83 0.71 0.74 6 0.9 1.79 0.62 2.0 0.80 0.83 0.78 0.83