Test Medium Reagent Positive Results Carbohydrate Glucose, maltose, Phenol red Red to Yellow fermentation test Sucrose, Lactose, (gas production Mannitol, Xylose in Durham’s tube) Urea broth, Phenol red Pinkish red Urea utilization test H2S Production 2% Peptone Lead acetate Blackening of test paper strip paper Nutrient gelatin Liquefaction at Gelatin hydrolysis – test broth 4°C Citrate utilization Simmons Citrate Bromothymole Green to Blue test agar Slant blue Peptone nitrate Sulphanilic acid Red Nitrate reduction test broth + a-Naphthalamine Oxidase test Nutrient Agar Slant Oxidase strip Violet color Nutrient Agar Slant 3% H2O2 Formation of Catalase test bubbles M-R test Glucose Phosphate Methyl red Red color broth Glucose Phosphate 40% KOH Pink color V-P test broth + a- Naphthol Iodole production 1% Tryptone reagent Red ring test production TSI slant Triple iron – – agar Slant Macconkey`s Agar Macconkeys agar – – plate plate METHOD
Test Medium Reagent Positive Results Carbohydrate fermentation test Glucose, maltose, Sucrose, Lactose, Mannitol, Xylose Phenol red Red to Yellow (gas production in Durham’s tube) Urea utilization test Urea broth, Phenol red Pinkish red H2S Production test 2% Peptone Lead acetate paper strip Blackening of paper Gelatin hydrolysis test Nutrient gelatin broth – Liquefaction at 4°C Citrate utilization test Simmons Citrate agar Slant Bromothymole blue Green to Blue Nitrate reduction test Peptone nitrate broth Sulphanilic acid + a-Naphthalamine Red Oxidase test Nutrient Agar Slant Oxidase strip Violet color Catalase test Nutrient Agar Slant 3% H2O2 Formation of bubbles M-R test Glucose Phosphate broth Methyl red Red color V-P test Glucose Phosphate broth 40% KOH + a- Naphthol Pink color Iodole production test 1% Tryptone reagent Red ring production TSI slant Triple iron agar Slant – – Macconkey`s Agar plate Macconkeys agar plate – – METHOD