for up to 4 days; considerable swelling and partial eversion of the eyelid in one animal; chemosis lasted for up to 4 days no corneal or iridal inflammation was observed the notified chemical was slightly irritating to the Result: eyes of rabbits Skin Sensitisation (Selbie, 1996) guinea pig/Dunkin Hartley Species/strain: test 10/sex Number of animals: control group 5/sex Induction procedure: test group: to a clipped area of the scapular dorsal skin, each day 1: animal received 3 pairs of 0.1 mL injections as follows – 1:1 (v/v) mixture of Freund’s Complete Adjuvant and 0.9 % (w/v) physiological saline the test material diluted to 1 % in corn oil the test material diluted to 2 % in corn oil, 1:1 (v/v) with Freund’s Complete Adjuvant; final test material concentration 1 % day 7 the same skin area was clipped and shaved and a filter patch saturated with 100 % test material was held in place under a polythene patch for 48 hours control group: day 1: to a clipped area of the scapular dorsal skin, each
for up to 4 days; considerable swelling and partial eversion of the eyelid in one animal; chemosis lasted for up to 4 days no corneal or iridal inflammation was observed Result: the notified chemical was slightly irritating to the eyes of rabbits Skin Sensitisation (Selbie, 1996) Species/strain: guinea pig/Dunkin Hartley Number of animals: test 10/sex control group 5/sex Induction procedure: test group: day 1: day 7 control group: day 1: to a clipped area of the scapular dorsal skin, each animal received 3 pairs of 0.1 mL injections as follows – 1:1 (v/v) mixture of Freund’s Complete Adjuvant and 0.9 % (w/v) physiological saline the test material diluted to 1 % in corn oil the test material diluted to 2 % in corn oil, 1:1 (v/v) with Freund’s Complete Adjuvant; final test material concentration 1 % the same skin area was clipped and shaved and a filter patch saturated with 100 % test material was held in place under a polythene patch for 48 hours to a clipped area of the scapular dorsal skin, each