Genotoxicity and Reverse Mutation Assay (HLS, Salmonella typhimurium Escherichia coli 1998e) TA1535, TA1537, TA98 TA100; Strains: Salmonella typhimurium CM891 Escherichia coli Initial test: 0, 5, 15, 50, 150, 500, 1 500, 5 000 g/plate; Concentration range: Repeat test: 0, 50, 150, 500, 1 500 and 5 000 g/plate; Test solutions were prepared in ethanol 10% and 20% (for initial and repeat assay, respectively) rat Metabolic activation: S9 fraction (Aroclor 1254-induced) in standard cofactors TA98 + S9: 5 benzo[a]pyrene; Positive controls: g/plate TA98 S9: 1g/plate 2-nitrofluorene; – TA100 + S9: 5 benzo[a]pyrene; g/plate TA100 S9: 3 N-ethyl-N’-nitro-N- g/plate – nitrosoguanidine TA1535 +S9: 2 2-aminoanthracene; g/plate TA1535 S9: 5 g/plate N-ethyl-N’-nitro-N – nitrosoguanidine; TA1537 + S9: 5 benzo[a]pyrene; g/plate TA1537 S9: 80 9-aminoacridine; g/plate – CM891 + S9: 10 2-aminoanthracene; g/plate CM891 S9: 2 N-ethyl-N’-nitro-N- g/plate – nitrosoguanidine. OECD TG 471 Test method: All concentrations were tested in triplicate. In both initial Comment: and repeat tests, there was inhibition of bacterial growth at 5 000 indicated by thinning of the background g/plate, lawn and reduction in revertant colony numbers. Under the conditions of the study, the notified chemical caused no substantial increases in revertant colony numbers over control counts at any concentration in either the presence or absence of the rat liver microsomal enzymes.
Genotoxicity Salmonella typhimurium and Escherichia coli Reverse Mutation Assay (HLS, 1998e) Strains: Salmonella typhimurium TA1535, TA1537, TA98 TA100; Escherichia coli CM891 Concentration range: Initial test: 0, 5, 15, 50, 150, 500, 1 500, 5 000 g/plate; Repeat test: 0, 50, 150, 500, 1 500 and 5 000 g/plate; Test solutions were prepared in ethanol Metabolic activation: 10% and 20% (for initial and repeat assay, respectively) rat S9 fraction (Aroclor 1254-induced) in standard cofactors Positive controls: TA98 + S9: 5 g/plate benzo[a]pyrene; TA98 – S9: 1g/plate 2-nitrofluorene; TA100 + S9: 5 g/plate benzo[a]pyrene; TA100 – S9: 3 g/plate N-ethyl-N’-nitro-N- nitrosoguanidine TA1535 +S9: 2 g/plate 2-aminoanthracene; TA1535 – S9: 5 g/plate N-ethyl-N’-nitro-N nitrosoguanidine; TA1537 + S9: 5 g/plate benzo[a]pyrene; TA1537 – S9: 80 g/plate 9-aminoacridine; CM891 + S9: 10 g/plate 2-aminoanthracene; CM891 – S9: 2 g/plate N-ethyl-N’-nitro-N- nitrosoguanidine. Test method: OECD TG 471 Comment: All concentrations were tested in triplicate. In both initial and repeat tests, there was inhibition of bacterial growth at 5 000 g/plate, indicated by thinning of the background lawn and reduction in revertant colony numbers. Under the conditions of the study, the notified chemical caused no substantial increases in revertant colony numbers over control counts at any concentration in either the presence or absence of the rat liver microsomal enzymes.