Appendix C: Environmental Fate and Ecotoxicological Investigations Environmental Fate Ultimate biodegradability Notified polymer OECD TG 301 F Ultimate Biodegradability: Manometric Respirometry Test. Inoculum Municipal sewage Exposure Period 28 days Auxiliary Solvent Water Analytical Monitoring Oxygen consumption / DOC Remarks - Method No significant protocol deviations were reported. Test substance Sodium acetate Day % Degradation Day % Degradation 7 39 7 75 14 59 14 85 21 73 21 87 28 78 28 87 Remarks - Results Additional substance specific analysis was done in accordance to note 3 at the MITI guidance document for testing the biodegradability of chemical substances by micro-organisms, to identify the degradation products and intermediates. According to the derivation procedure described in the annex determination of Sovermol 1102’ all alcoholic ‘analytical hydroxylgroups were derivated to the corresponding trimethylsilyl (TMS) ethers, which can directly be separated by gas chromatography. It could be shown that in contrast to the good recovery rate of the notified polymer at the test start, neither the hydrophobic parent-substances nor other hydrophobic compounds were traceable in the final samples. Only one of three parallel flasks showed small remains (<2%) of the notified polymer. In order to determine polar metabolites, further investigations were made. Although it was not possible to identify the exact structure of these substances, their high polarity and their low molecular weight are indicative for them being degradation products. Thus the notified polymer appears to have been almost completely mineralised, respectively fixed in biomass within the examined test period of 28 days. The notified polymer is ultimately biodegradable. Henkel KGaA (1997) Ecotoxicological Investigations Acute toxicity to aquatic invertebrates Notified polymer OECD TG 202 Daphnia sp. Acute Immobilisation Test and Reproduction Test - Static. EC Directive 92/69/EEC C.2 Toxicity for Daphnia Static. – Species Daphnia magna Exposure Period 48 hours Hardness 267 mg CaCO3/L
Appendix C: Environmental Fate and Ecotoxicological Investigations Environmental Fate Ultimate biodegradability Notified polymer OECD TG 301 F Ultimate Biodegradability: Manometric Respirometry Test. Inoculum Municipal sewage Exposure Period 28 days Auxiliary Solvent Water Analytical Monitoring Oxygen consumption / DOC Remarks - Method No significant protocol deviations were reported. Test substance Sodium acetate Day % Degradation Day % Degradation 7 39 7 75 14 59 14 85 21 73 21 87 28 78 28 87 Remarks - Results Additional substance specific analysis was done in accordance to note 3 at the MITI guidance document for testing the biodegradability of chemical substances by micro-organisms, to identify the degradation products and intermediates. According to the derivation procedure described in the annex ‘analytical determination of Sovermol 1102’ all alcoholic hydroxylgroups were derivated to the corresponding trimethylsilyl (TMS) ethers, which can directly be separated by gas chromatography. It could be shown that in contrast to the good recovery rate of the notified polymer at the test start, neither the hydrophobic parent-substances nor other hydrophobic compounds were traceable in the final samples. Only one of three parallel flasks showed small remains (<2%) of the notified polymer. In order to determine polar metabolites, further investigations were made. Although it was not possible to identify the exact structure of these substances, their high polarity and their low molecular weight are indicative for them being degradation products. Thus the notified polymer appears to have been almost completely mineralised, respectively fixed in biomass within the examined test period of 28 days. The notified polymer is ultimately biodegradable. Henkel KGaA (1997) Ecotoxicological Investigations Acute toxicity to aquatic invertebrates Notified polymer OECD TG 202 Daphnia sp. Acute Immobilisation Test and Reproduction Test - Static. EC Directive 92/69/EEC C.2 Toxicity for Daphnia – Static. Species Daphnia magna Exposure Period 48 hours Hardness 267 mg CaCO3/L