Positive control group animals were sacrificed 24 hours after dosing. No mortality; Clinical observations: No clinical signs of toxicity; No significant increase in micronucleated polychromatic Micronuclei score: erythrocytes (PCE) due to treatment with test substance at either sampling time; the positive control caused a significant increase in micronucleated PCE. OECD TG 474 Test method: Gulftene 12-16 did not induce a significant increase in Result: micronucleated PCEs in bone marrow cells of the mouse in vivo. 9.3.2.2 Mammalian Erythrocyte Micronucleus Test Using C20-C24 alkenes, branched and linear (Safepharm Laboratories Limited 1998) C20-C24 alkenes, branched and linear Test substance: mouse/Crl:CD-1 (ICR) BR Species/strain: 7 males/24 hour, vehicle and positive control, and mid, Number and sex of animals: low and high dose group; 7 males/48 hour, vehicle control and high dose group; 5 males/positive control group. Test substance: 500 mg/kg (low), 1 000 mg/kg (mid) or Doses/Method of 2 000 mg/kg (high); administration: Positive control: cyclophosphamide 50 mg/kg; Vehicle control: arachis oil; All administered via intraperitoneal injection at a constant volume of 10 mL/kg bw. Positive control was administered orally. Vehicle and positive control, low, mid and high dose Sampling schedule: animals were sacrificed 24 hours after dosing. Remaining animals of the vehicle control group and high dose animals were sacrificed 48 hours after dosing. No mortality. Clinical observations: No clinical signs of toxicity. No significant increase in micronucleated PCE due to Micronuclei score: treatment with test substance at either sampling time. No statistically significant decrease in the PCE/NCE ratio at 24 or 48 hours.
Positive control group animals were sacrificed 24 hours after dosing. Clinical observations: No mortality; No clinical signs of toxicity; Micronuclei score: No significant increase in micronucleated polychromatic erythrocytes (PCE) due to treatment with test substance at either sampling time; the positive control caused a significant increase in micronucleated PCE. Test method: OECD TG 474 Result: Gulftene 12-16 did not induce a significant increase in micronucleated PCEs in bone marrow cells of the mouse in vivo. 9.3.2.2 Mammalian Erythrocyte Micronucleus Test Using C20-C24 alkenes, branched and linear (Safepharm Laboratories Limited 1998) Test substance: C20-C24 alkenes, branched and linear Species/strain: mouse/Crl:CD-1 (ICR) BR Number and sex of animals: 7 males/24 hour, vehicle and positive control, and mid, low and high dose group; 7 males/48 hour, vehicle control and high dose group; 5 males/positive control group. Doses/Method of administration: Test substance: 500 mg/kg (low), 1 000 mg/kg (mid) or 2 000 mg/kg (high); Positive control: cyclophosphamide 50 mg/kg; Vehicle control: arachis oil; All administered via intraperitoneal injection at a constant volume of 10 mL/kg bw. Positive control was administered orally. Sampling schedule: Vehicle and positive control, low, mid and high dose animals were sacrificed 24 hours after dosing. Remaining animals of the vehicle control group and high dose animals were sacrificed 48 hours after dosing. Clinical observations: No mortality. No clinical signs of toxicity. Micronuclei score: No significant increase in micronucleated PCE due to treatment with test substance at either sampling time. No statistically significant decrease in the PCE/NCE ratio at 24 or 48 hours.