ASSESSMENT OF ENVIRONMENTAL EFFECTS The following ecotoxicity studies have been supplied by the notifier for dyestuff, containing Reactive Red 202 ester, vinyl and hydroxy components. Tests were performed according to OECD Test Guidelines at facilities complying with OECD Principles of Good Laboratory Practice. Test Species Results (Nominal) Reference Carp mg.L-1 Acute Toxicity (Cyprinus carpio) NOEC = 1 000 (19) 96 h Semi-static Water Flea mg.L-1 Acute Immobilisation (Daphnia magna) EC = 652 mg.L-1 (20) 48 h Static NOEC = 320 mg.L-1 Water Flea Chronic Toxicity (Daphnia magna) Reproduction (21) 21 days Semi-static EC = 22 NOEC = 6 mg.L-1 Parent Mortality LC = 97 mg.L-1 Growth Inhibition: NOEC = 18 (Scenedesmus (22) Growth Biomass , b EC > 100 mg.L-1 LC50 > 1 000 NOEC() = 4 mg.L-1 72 h Static = 32 mg.L-1 0 mg.L-1 Respiration (23) Aerobic Waste IC > 100 mg.L-1 Inhibition Water Bacteria NOEC > 100 0.5 h mg.L-1 NOEC(b) = 4 Fish Based on the results of the range finding test, the final study was a limit test exposing carp to nominal concentrations of 1 000 mg.L-1. The test media were renewed every 24 hours, with final test solutions clear red without precipitation. The concentration of the total mixture remained greater than 90% during the 24 hour period of renewal. Results of analysis indicated that the concentration of Reactive Red 202 (ester) decreased but remained greater than 80% over 24 hours. The concentration of Reactive Red 202 (vinyl) increased almost proportionally to the decrease of the ester. This finding indicated that Reactive Red 202 (ester) is converted to Reactive Red 202 (vinyl). During the 96 hour exposure period, no mortality of fish or any effects were observed. In conclusion, the 96 h LC50 for carp indicates that the notified chemical is practically non-toxic to fish. Aquatic Invertebrates Acute Daphnia were exposed to nominal concentrations ranging from 100 to
ASSESSMENT OF ENVIRONMENTAL EFFECTS The following ecotoxicity studies have been supplied by the notifier for dyestuff, containing Reactive Red 202 ester, vinyl and hydroxy components. Tests were performed according to OECD Test Guidelines at facilities complying with OECD Principles of Good Laboratory Practice. Test Species Results (Nominal) Reference Acute Toxicity 96 h Semi-static Acute Immobilisation 48 h Static Chronic Toxicity 21 days Semi-static Growth Inhibition: Growth , Biomass b Carp (Cyprinus carpio) Water Flea (Daphnia magna) Water Flea (Daphnia magna) (Scenedesmus LC50 > 1 000 mg.L-1 NOEC = 1 000 mg.L-1 EC = 652 mg.L-1 NOEC = 320 mg.L-1 Reproduction EC = 22 NOEC = 6 mg.L-1 Parent Mortality LC = 97 mg.L-1 NOEC = 18 EC > 100 mg.L-1 NOEC() = 4 mg.L-1 (19) (20) (21) (22) 72 h Static = 32 mg.L-1 Respiration Inhibition 0.5 h Aerobic Waste Water Bacteria NOEC(b) = 4 mg.L-1 IC > 100 mg.L-1 NOEC > 100 mg.L-1 (23) Fish Based on the results of the range finding test, the final study was a limit test exposing carp to nominal concentrations of 1 000 mg.L-1. The test media were renewed every 24 hours, with final test solutions clear red without precipitation. The concentration of the total mixture remained greater than 90% during the 24 hour period of renewal. Results of analysis indicated that the concentration of Reactive Red 202 (ester) decreased but remained greater than 80% over 24 hours. The concentration of Reactive Red 202 (vinyl) increased almost proportionally to the decrease of the ester. This finding indicated that Reactive Red 202 (ester) is converted to Reactive Red 202 (vinyl). During the 96 hour exposure period, no mortality of fish or any effects were observed. In conclusion, the 96 h LC50 for carp indicates that the notified chemical is practically non-toxic to fish. Aquatic Invertebrates Acute Daphnia were exposed to nominal concentrations ranging from 100 to 0