APPENDIX II: FACS Equipment on the ORC Flow Details Housed Current Notes Accessible/charges Cytometers Beckman Will be moved to Coulter - Cyan TDI Building Currently Housed in WIMM flow £35h unassisted/ £80h ADP Violet (2), Blue (5), Red (2) plus FSC and SSC 2013 70-80% cytometry facility assisted LSRII (BD) 3 lasers Blue/Red/Violet Jenner/ORCRB 80% Yes: 15 parameters plus FSC and SSC Beckman 3 lasers Blue/Red/Violet Jenner/ORCRB 20% Non-Jenner £80 ph unassisted / £100 Yes Coulter CyAn 9 fluorescent parameters in addition to FSC and ph assisted. ADP LX SSC Discounted price for WTCHGU £54 / £27 ph respectively. BD FACSCalibur 2 lasers Blue/Red Jenner/ORCRB Yes 4 fluorescent parameters in addition to FSC and SSC, 2000 events per second FACSCanto 2 lasers Blue/Red in addition to FSC and SSC Ludwig/ORCRB 30% Yes (BD) FACSCanto 2 lasers Blue/Red in addition to FSC and SSC Cornall lab/ 80% Fees for external users at Yes (BD) CCMP discretion of R Cornall BD LSRII 3 lasers Blue / Red/ upgrade with Violet Kennedy 5 years old (might do a laser upgrade Free for Kennedy users with violet) BD FACSCanto 3 lasers Blue/ Red/Violet Kennedy 80% 5 years old Free for Kennedy users II with HTS 8 fluorescent parameters in addition to FSC and No upgrade possible SSC HTS possible
APPENDIX II: FACS Equipment on the ORC Flow Cytometers Details Housed Current Notes Accessible/charges Beckman Coulter - Cyan ADP Violet (2), Blue (5), Red (2) plus FSC and SSC Will be moved to TDI Building 2013 70-80% Currently Housed in WIMM flow cytometry facility £35h unassisted/ £80h assisted LSRII (BD) 3 lasers Blue/Red/Violet 15 parameters plus FSC and SSC Jenner/ORCRB 80% Yes: Beckman Coulter CyAn ADP LX 3 lasers Blue/Red/Violet 9 fluorescent parameters in addition to FSC and SSC Jenner/ORCRB 20% Non-Jenner £80 ph unassisted / £100 ph assisted. Discounted price for WTCHGU £54 / £27 ph respectively. Yes BD FACSCalibur 2 lasers Blue/Red 4 fluorescent parameters in addition to FSC and SSC, 2000 events per second Jenner/ORCRB Yes FACSCanto (BD) 2 lasers Blue/Red in addition to FSC and SSC Ludwig/ORCRB 30% Yes FACSCanto (BD) 2 lasers Blue/Red in addition to FSC and SSC Cornall lab/ CCMP 80% Fees for external users at discretion of R Cornall Yes BD LSRII 3 lasers Blue / Red/ upgrade with Violet Kennedy 5 years old (might do a laser upgrade with violet) Free for Kennedy users BD FACSCanto II with HTS 3 lasers Blue/ Red/Violet 8 fluorescent parameters in addition to FSC and SSC Kennedy 80% 5 years old No upgrade possible HTS possible Free for Kennedy users