Supplementary Materials: Table S1. Samples used for RAD-seq experiment. Coordinates are measured and accurate to 100m except for Saskatchewan and Northwest Territories (NWT) where specific locations are not known and estimates are centralized but varied so they do not overlap when mapping. The AB Gray Wolf is from Jasper National Park and co-ordinates are estimated near the town of Jasper. NRDPFC = Natural Resources DNA Profiling and Forensic Centre; APP=Algonquin Provincial Park. NRDPFC Type Location Latitude Longitude Canis Sample ID Northwest CAN001024 Gray Wolf 62.518022 -114.366302 Territories Northwest CAN001051 Gray Wolf 62.446324 -114.498138 Territories Northwest CAN001055 Gray Wolf 62.415816 -114.436340 Territories DB179893 Gray Wolf Alberta 52.867472 -117.978058 CAN002356 Western Coyote Saskatchewan 50.733848 -109.048920 CAN002360 Western Coyote Saskatchewan 50.864044 -106.824188 CAN002369 Western Coyote Saskatchewan 50.863178 -104.959259 CAN004255 Eastern Wolf Ontario - APP 45.496133 -78.429533 CAN004534 Eastern Wolf Ontario - APP 45.580231 -78.390554 DB179508 Eastern Wolf Ontario - APP DB179509 Eastern Wolf Ontario - APP 45.33048 Ontario - CAN004210 Eastern Coyote 44.200463 -81.149188 Southern Ontario - CAN004216 Eastern Coyote 44.061691 -80.653732 Southern Ontario - CAN004232 Eastern Coyote 44.108311 -80.556987 Southern Great Ontario - CAN004172 49.087152 -91.534080 Wolf Northern Great Ontario - CAN004186 49.766775 -86.460085 Wolf Northern Great Ontario - CAN004243 49.217126 -80.723843 Wolf Northern
Supplementary Materials: Table S1. Samples used for RAD-seq experiment. Coordinates are measured and accurate to 100m except for Saskatchewan and Northwest Territories (NWT) where specific locations are not known and estimates are centralized but varied so they do not overlap when mapping. The AB Gray Wolf is from Jasper National Park and co-ordinates are estimated near the town of Jasper. NRDPFC = Natural Resources DNA Profiling and Forensic Centre; APP=Algonquin Provincial Park. NRDPFC Sample ID Canis Type Location Latitude Longitude CAN001024 Gray Wolf Northwest Territories 62.518022 -114.366302 CAN001051 Gray Wolf Northwest Territories 62.446324 -114.498138 CAN001055 Gray Wolf Northwest Territories 62.415816 -114.436340 DB179893 Gray Wolf Alberta 52.867472 -117.978058 CAN002356 Western Coyote Saskatchewan 50.733848 -109.048920 CAN002360 Western Coyote Saskatchewan 50.864044 -106.824188 CAN002369 Western Coyote Saskatchewan 50.863178 -104.959259 CAN004255 Eastern Wolf Ontario - APP 45.496133 -78.429533 CAN004534 Eastern Wolf Ontario - APP 45.580231 -78.390554 DB179508 Eastern Wolf Ontario - APP DB179509 Eastern Wolf Ontario - APP 45.33048 CAN004210 Eastern Coyote Ontario - Southern 44.200463 -81.149188 CAN004216 Eastern Coyote Ontario - Southern 44.061691 -80.653732 CAN004232 Eastern Coyote Ontario - Southern 44.108311 -80.556987 CAN004172 Great Wolf Ontario - Northern 49.087152 -91.534080 CAN004186 Great Wolf Ontario - Northern 49.766775 -86.460085 CAN004243 Great Wolf Ontario - Northern 49.217126 -80.723843