Haematoxylin and Eosin 1 Dip the sections in different alcohol concentrations finishing with water as last – passage 2 Haematoxylin (no longer than 5/6 min) – Stain using Harris’ 3 Wash in water – 4 Differentiate in 1% acid alcohol (5/10 sec) (1% HCl) – 5 Wash in water – 6 Make it blue using tap water (1/2 min) – 7 Wash in water – 8 Examine sections in microscope – If sections too differentiate repeat from step 2 If sections under differentiate repeat from step 4 9 Stain in alcoholic aqueous eosin (no longer than 2/5 min) – 10 Wash in water – 11 Dehydrate, clear and check again in the microscope – 12 Mount using a resinous mountant (national museums xylene mountant or dpx) – Results: Nuclei blue Cytoplasm and other structures shades of pink
Haematoxylin and Eosin 1 – Dip the sections in different alcohol concentrations finishing with water as last passage 2 – Stain using Harris’ Haematoxylin (no longer than 5/6 min) 3 – Wash in water 4 – Differentiate in 1% acid alcohol (5/10 sec) (1% HCl) 5 – Wash in water 6 – Make it blue using tap water (1/2 min) 7 – Wash in water 8 – Examine sections in microscope If sections too differentiate repeat from step 2 If sections under differentiate repeat from step 4 9 – Stain in alcoholic aqueous eosin (no longer than 2/5 min) 10 – Wash in water 11 – Dehydrate, clear and check again in the microscope 12 – Mount using a resinous mountant (national museums xylene mountant or dpx) Results: Nuclei blue Cytoplasm and other structures shades of pink