supplemented with a significant concentration of KH2PO4 (1.6 mg/L, equivalent to 0.36 mg total P/L). The analytical monitoring results for the notified chemical in this study are therefore not considered reliable for regulatory purposes. Growth Biomass ErL50 EbL50 mg/L at (0-72)h > 100 mg/L (based on Not determined loading rates) Remarks - Results All validity criteria for the test were satisfied. The toxic response of algae to the reference compound K2Cr2O7 gave an ErC50 (0 - 72 h) of 0.69 mg/L which was consistent with the historical data for the laboratory. The maximum inhibition of algal growth observed for the test substance was 6.9% hence the ErL50 lies above the maximum loading rate of 100 mg/L. The statistical significance of the observed inhibition of growth rate was not calculated, hence the NOErL could not be reliably reported. However, the inhibition of average specific growth rate was not dose responsive and was in a low range between -3.6 and 2.7%. The test substance, and by inference the notified chemical, is not harmful to algae Unpublished (2008l) Inhibition of microbial activity Sensanov WR (50-60% notified chemical) OECD TG 209 Activated Sludge, Respiration Inhibition Test. EC Directive 88/302/EEC C.11 Biodegradation: Activated Sludge Respiration Inhibition Test Inoculum Activated sewage sludge microorganisms from a sewage treatment treating predominantly domestic sewage Exposure Period 3 hours Nominal: 1000 mg/L Actual: Not reported Remarks Method a range finding test, the definitive test was conducted according to – the guidelines above at a test substance loading level of 1000 mg/L dispersed in dechlorinated tap water with the addition of a synthetic sewage as a respiratory substrate. A blank control and reference control were run in parallel. The rate of respiration was determined after 3 h contact time and compared to the results from the control and reference material. Test conditions: 21°C, pH 7.6-7.9. IL50 > 1000 mg/L NOEL 1000 mg/L Remarks Results All validity criteria for the test were satisfied. Some dissolved oxygen – concentrations were below those recommended in the test guideline but this was not considered to adversely affect the results of the study given that in all the cases the oxygen consumption was determined over the linear portion of the oxygen consumption trace.
supplemented with a significant concentration of KH2PO4 (1.6 mg/L, equivalent to 0.36 mg total P/L). The analytical monitoring results for the notified chemical in this study are therefore not considered reliable for regulatory purposes. Growth Biomass ErL50 EbL50 mg/L at (0-72)h > 100 mg/L (based on loading rates) Not determined Remarks - Results All validity criteria for the test were satisfied. The toxic response of algae to the reference compound K2Cr2O7 gave an ErC50 (0 - 72 h) of 0.69 mg/L which was consistent with the historical data for the laboratory. The maximum inhibition of algal growth observed for the test substance was 6.9% hence the ErL50 lies above the maximum loading rate of 100 mg/L. The statistical significance of the observed inhibition of growth rate was not calculated, hence the NOErL could not be reliably reported. However, the inhibition of average specific growth rate was not dose responsive and was in a low range between -3.6 and 2.7%. The test substance, and by inference the notified chemical, is not harmful to algae Unpublished (2008l) Inhibition of microbial activity Sensanov WR (50-60% notified chemical) OECD TG 209 Activated Sludge, Respiration Inhibition Test. EC Directive 88/302/EEC C.11 Biodegradation: Activated Sludge Respiration Inhibition Test Inoculum Activated sewage sludge microorganisms from a sewage treatment treating predominantly domestic sewage Exposure Period 3 hours Nominal: 1000 mg/L Actual: Not reported Remarks – Method a range finding test, the definitive test was conducted according to the guidelines above at a test substance loading level of 1000 mg/L dispersed in dechlorinated tap water with the addition of a synthetic sewage as a respiratory substrate. A blank control and reference control were run in parallel. The rate of respiration was determined after 3 h contact time and compared to the results from the control and reference material. Test conditions: 21°C, pH 7.6-7.9. IL50 > 1000 mg/L NOEL 1000 mg/L Remarks – Results All validity criteria for the test were satisfied. Some dissolved oxygen concentrations were below those recommended in the test guideline but this was not considered to adversely affect the results of the study given that in all the cases the oxygen consumption was determined over the linear portion of the oxygen consumption trace.