Procedure for Cleaning Flow Cell Wyatt Light Scattering July 22, 2008 Barbara White Signs of Dirty Flow Cell: Baseline noise: Should be 0.2-0.3 aqueous 0.7-0.8 for THF 2.8 for toluene Wobbling baseline 3-D plots show offsets Flow Cell Cleaning (Mobile Phase Flushing) Not Using a Column Flush 20 mL of 100% alcohol (ethanol, methanol, isopropyl alcohol or a mix of these). Then follow with water. If mobile phase flushing does not work: 20% nitric acid---leave in for two hours to overnight Use Oral B Superfloss to clean the bore of the flow cell glass. 1% Alconox solution and a gloved finger can be used to clean the small windows. The manifold can also be cleaned with Alconox. After cleaning, rinse with isopropyl alcohol and then dry with nitrogen gas.
Procedure for Cleaning Flow Cell Wyatt Light Scattering July 22, 2008 Barbara White Signs of Dirty Flow Cell: Baseline noise: Should be 0.2-0.3 aqueous 0.7-0.8 for THF 2.8 for toluene Wobbling baseline 3-D plots show offsets Flow Cell Cleaning (Mobile Phase Flushing) Not Using a Column Flush 20 mL of 100% alcohol (ethanol, methanol, isopropyl alcohol or a mix of these). Then follow with water. If mobile phase flushing does not work: 20% nitric acid---leave in for two hours to overnight Use Oral B Superfloss to clean the bore of the flow cell glass. 1% Alconox solution and a gloved finger can be used to clean the small windows. The manifold can also be cleaned with Alconox. After cleaning, rinse with isopropyl alcohol and then dry with nitrogen gas.