Ecotoxicity Test Results Test Species Results (Nominal) Acute Toxicity (static test) Zebra fish 96 h LC50 = 67.0 mg/L (OECD 203) 96 h LC0 = 32.5 mg/L Brachydanio rerio Acute Toxicity -Immobilisation Water Flea 48 h EC50 = 33.5 mg/L Test (Static Test) (calculated) Daphnia magna (OECD TG 202) 48 h NOEC mg/L 5.8 -Growth Growth Inhibition () Green Algae 72 h EbC50 = 26.4 mg/L & Biomass (b) (Static Test) 72 h NOEC()< 3.7 mg/L Scenedesmus subspicatus (OECD TG 201) Bacteria Respiration Inhibition Activated Sludge - (OECD TG 209) Aerobic Water 3 h IC50 > 320 mg/L Earthworm Toxicity to earthworms 14 d EC50 < 1 000 Eisenia foetida (OECD TG 207) mg/kg (calculated) foetida Comments on Ecotoxicity testing Fish A static test was performed in accordance with the test guidelines. Nominal concentrations of 10, 17.8, 32, 56 and 100 mg/L and a control were tested in parallel. All reported results are related to nominal concentrations as the test substance was sufficiently stable during the test period. The analytically determined concentrations varied from 97% to 110% of the nominal values. Results demonstrated that the notified substance had slightly toxic effects on the test fish with a 96 h LC50 = 67.0 mg/L. No deaths occurred at 32 mg/L, two fish died at 56 mg/L and all ten fish in the 100 mg/L treatment died. The results of the test did not allow a probit analysis to check the calculations of the LC50 as there was only one point between 0 and 100% mortality. Where deaths are measured, this type of analysis requires at least two points. All control fish survived until the end of the test and no abnormal responses of the fish were observed during testing. Aquatic Invertebrates Nominal concentrations of 5.8, 10, 18, 32, 58 and 100 mg/L and a control were tested in parallel. All reported results are related to nominal concentrations as the test substance was sufficiently stable during the test period. The analytically determined concentrations varied from 97% to 100% of the nominal values.
Ecotoxicity Test Results Test Species Results (Nominal) Acute Toxicity (static test) (OECD 203) Zebra fish Brachydanio rerio 96 h LC50 = 67.0 mg/L 96 h LC0 = 32.5 mg/L Acute Toxicity -Immobilisation Test (Static Test) (OECD TG 202) Water Flea Daphnia magna 48 h EC50 = 33.5 mg/L (calculated) 48 h NOEC 5.8 mg/L Growth Inhibition -Growth () & Biomass (b) (Static Test) (OECD TG 201) Green Algae Scenedesmus subspicatus 72 h EbC50 = 26.4 mg/L 72 h NOEC()< 3.7 mg/L Respiration Inhibition (OECD TG 209) Activated Sludge - Aerobic Water Bacteria 3 h IC50 > 320 mg/L Toxicity to earthworms (OECD TG 207) Earthworm Eisenia foetida foetida 14 d EC50 < 1 000 mg/kg (calculated) Comments on Ecotoxicity testing Fish A static test was performed in accordance with the test guidelines. Nominal concentrations of 10, 17.8, 32, 56 and 100 mg/L and a control were tested in parallel. All reported results are related to nominal concentrations as the test substance was sufficiently stable during the test period. The analytically determined concentrations varied from 97% to 110% of the nominal values. Results demonstrated that the notified substance had slightly toxic effects on the test fish with a 96 h LC50 = 67.0 mg/L. No deaths occurred at 32 mg/L, two fish died at 56 mg/L and all ten fish in the 100 mg/L treatment died. The results of the test did not allow a probit analysis to check the calculations of the LC50 as there was only one point between 0 and 100% mortality. Where deaths are measured, this type of analysis requires at least two points. All control fish survived until the end of the test and no abnormal responses of the fish were observed during testing. Aquatic Invertebrates Nominal concentrations of 5.8, 10, 18, 32, 58 and 100 mg/L and a control were tested in parallel. All reported results are related to nominal concentrations as the test substance was sufficiently stable during the test period. The analytically determined concentrations varied from 97% to 100% of the nominal values.