Acknowledgements Please list any acknowledgements you wish to make here. References List of cited literature: please do not include urls here, but rather add them to the main manuscript. Tables Please add tables here. See below for some examples (taken from ) Table 1: Details of ancillary reagents for immunohistochemistry Catalogue Process Reagent Manufacturer Concentration Number Neutral Buffered Fixation Sigma HT501128 10% Formaldehyde ER1 (Sodium Leica Biosystems AR9961 Proprietary Citrate, pH6) ER2 (Tris/EDTA, Pretreatment Leica Biosystems AR9640 Proprietary pH9) Enzyme 1 Leica Biosystems AR9551 100µg/ml (Proteinase K) Peroxide Block Leica Biosystems DS9263 Proprietary Streptavidin – HRP Leica Biosystems DS9263 Proprietary Diaminobenizidine Staining Leica Biosystems DS9263 Proprietary (DAB) Haematoxylin Leica Biosystems DS9263 Proprietary DAB Enhancer Leica Biosystems AR9452 Proprietary Bond Wash (Tris Leica Biosystems AR9590 Proprietary Buffer) Washes/Blocks Antibody Diluent Leica Biosystems AR9352 Proprietary Vector Avidin/Biotin Block SP-2001 Proprietary Laboratories Table 2: Details of primary and secondary antibodies. Antibody Manufacturer Catalogue Number RRID ARID1A Atlas Antibodies HPA005456 AB_1078205 GAPDH (Clone 14C10) Cell Signaling 2118 AB_561053
Acknowledgements Please list any acknowledgements you wish to make here. References List of cited literature: please do not include urls here, but rather add them to the main manuscript. Tables Please add tables here. See below for some examples (taken from ) Table 1: Details of ancillary reagents for immunohistochemistry Process Reagent Manufacturer Catalogue Number Concentration Fixation Neutral Buffered Formaldehyde Sigma HT501128 10% Pretreatment ER1 (Sodium Citrate, pH6) Leica Biosystems AR9961 Proprietary ER2 (Tris/EDTA, pH9) Leica Biosystems AR9640 Proprietary Enzyme 1 (Proteinase K) Leica Biosystems AR9551 100µg/ml Staining Peroxide Block Leica Biosystems DS9263 Proprietary Streptavidin – HRP Leica Biosystems DS9263 Proprietary Diaminobenizidine (DAB) Leica Biosystems DS9263 Proprietary Haematoxylin Leica Biosystems DS9263 Proprietary DAB Enhancer Leica Biosystems AR9452 Proprietary Washes/Blocks Bond Wash (Tris Buffer) Leica Biosystems AR9590 Proprietary Antibody Diluent Leica Biosystems AR9352 Proprietary Avidin/Biotin Block Vector Laboratories SP-2001 Proprietary Table 2: Details of primary and secondary antibodies. Antibody Manufacturer Catalogue Number RRID ARID1A Atlas Antibodies HPA005456 AB_1078205 GAPDH (Clone 14C10) Cell Signaling 2118 AB_561053