TABLE OF CONTENTS Page Chapter I Introduction and Background 1 Cellular Components 2 Immunostaining 9 II Materials and Methods 14 Creating of substrates for TFM 14 Primary mouse keratinocyte 15 Cell Staining 16 III Results 18 Cellular images 29 Focal adhesion quantification 26 IV Discussion 29 Quantitative explanations 30 Future Studies 38 Appendices 40 Appendix A IACUC Approval of Study 41 – Appendix B Quantification of Focal Adhesions 43 – References 47
TABLE OF CONTENTS Page Chapter I Introduction and Background 1 Cellular Components 2 Immunostaining 9 II Materials and Methods 14 Creating of substrates for TFM 14 Primary mouse keratinocyte 15 Cell Staining 16 III Results 18 Cellular images 29 Focal adhesion quantification 26 IV Discussion 29 Quantitative explanations 30 Future Studies 38 Appendices 40 Appendix A – IACUC Approval of Study 41 Appendix B – Quantification of Focal Adhesions 43 References 47