Lesion Mean Maximum Value Maximum Duration Maximum Value at of Any Effect End of Observation Period 2 2 72 hr 1 Erythema/Eschar 0.7 1 24 hr 0 Oedema *Calculated on the basis of the scores at 24 and 72 hours for ALL animals. Remarks Results Test substance caused slight to moderate skin irritation. Its dermal effects – generally consisted of very slight or well-defined erythema, very slight oedema and slight scaliness. The notified chemical is moderately irritating to the skin and is classified under the NOHSC Approved Criteria for Classifying Hazardous Substances. CIVO Institutes TNO (1981a) 7.5. Irritation eye – Notified chemical FDA standard Species/Strain Rabbit/New Zealand White Number of Animals 6 Observation Period 48 hours Remarks Method 0.1 mL of the test substance is allowed to fall on the everted lower lid of – one eye of each rabbit; the upper and lower eye lid are then carefully closed and subsequently held together for at least one second before releasing, to prevent loss of the test substance. The other eye, remaining untreated, serves as a control. The eyes are examined at 24, 48, 72 hours and 7 days after instillation of the test substance. Lesion Mean Maximum Maximum Maximum Value at Duration of Any End of Observation Effect Period 0.08 1 24 hr 0 Conjunctiva: redness 0.08 1 24 hr 0 Conjunctiva: chemosis 0 0 - 0 Corneal opacity 0 0 - 0 Iridial inflammation *Calculated on the of the scores at 24 and 48 hours for ALL animals. Remarks Results Test substance caused slight conjunctivitis in one out of 12 rabbits. After – 48 hours these lesions had cleared up. The notified chemical is slightly irritating to the eye. CIVO Institutes TNO ( 1981a) 7.6. Skin sensitisation Notified chemical Guinea pig maximisation test by B. Magnusson and AM Kligman (1970) in Allergic Contact Dermatitis in the Guinea pig: identification of contact allergens”, published by C.C. Thomas, Springfield, Illinois, USA. Species/Strain Guinea pig/albino Hartley/Dunkin preliminary study Maximum Non-irritating Concentration:
Lesion Mean Maximum Value Maximum Duration of Any Effect Maximum Value at End of Observation Period Erythema/Eschar 2 0.7 2 1 72 hr 24 hr 1 0 Oedema *Calculated on the basis of the scores at 24 and 72 hours for ALL animals. Remarks – Results Test substance caused slight to moderate skin irritation. Its dermal effects generally consisted of very slight or well-defined erythema, very slight oedema and slight scaliness. The notified chemical is moderately irritating to the skin and is classified under the NOHSC Approved Criteria for Classifying Hazardous Substances. CIVO Institutes TNO (1981a) 7.5. Irritation – eye Notified chemical FDA standard Species/Strain Rabbit/New Zealand White Number of Animals 6 Observation Period 48 hours Remarks – Method 0.1 mL of the test substance is allowed to fall on the everted lower lid of one eye of each rabbit; the upper and lower eye lid are then carefully closed and subsequently held together for at least one second before releasing, to prevent loss of the test substance. The other eye, remaining untreated, serves as a control. The eyes are examined at 24, 48, 72 hours and 7 days after instillation of the test substance. Lesion Mean Maximum Maximum Duration of Any Effect Maximum Value at End of Observation Period Conjunctiva: redness 0.08 1 24 hr 0 Conjunctiva: chemosis 0.08 1 24 hr 0 Corneal opacity 0 0 - 0 Iridial inflammation 0 0 - 0 *Calculated on the of the scores at 24 and 48 hours for ALL animals. Remarks – Results Test substance caused slight conjunctivitis in one out of 12 rabbits. After 48 hours these lesions had cleared up. The notified chemical is slightly irritating to the eye. CIVO Institutes TNO ( 1981a) 7.6. Skin sensitisation Notified chemical Guinea pig maximisation test by B. Magnusson and AM Kligman (1970) in Allergic Contact Dermatitis in the Guinea pig: identification of contact allergens”, published by C.C. Thomas, Springfield, Illinois, USA. Species/Strain Guinea pig/albino Hartley/Dunkin preliminary study Maximum Non-irritating Concentration: