<s>[INST] Microbiology Lab 5 - Aseptic Technique and Sterilization
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**Lab Objectives:**

*   Understand the importance of aseptic technique in microbiology
*   Learn various methods of sterilization and their applications
*   Practice aseptic technique and sterilization procedures

**Materials Needed:**

*   Bacterial cultures (e.g., E. coli, S. aureus)
*   Agar plates
*   Petri dishes
*   Inoculation loops
*   Autoclave
*   Bunsen burner
*   Incubator
*   Gloves
*   Lab coat

**Introduction:**

Aseptic technique is a crucial aspect of microbiology that involves handling microorganisms in a way that prevents contamination and ensures the accuracy of results. In this lab, we will explore the principles of aseptic technique and various methods of sterilization.

**Procedure:**

### Aseptic Technique

1.  **Preparation:** Put on gloves and a lab coat to prevent skin contact and contamination.
2.  **Handling Cultures:** Use an inoculation loop to handle bacterial cultures. Flaming the loop before and after use can help prevent contamination.
3.  **Inoculating Agar Plates:** Open the agar plate and inoculate it with the bacterial culture using the inoculation loop. Close the plate immediately after inoculation.
4.  **Incubation:** Place the inoculated agar plate in the incubator at 37°C for 24-48 hours.

### Sterilization Methods

1.  **Autoclaving:** Place the materials to be sterilized in the autoclave and set the temperature to 121°C for 15-20 minutes.
2.  **Flaming:** Hold the inoculation loop in the flame of the Bunsen burner until it is red-hot. Allow it to cool before use.
3.  **Dry Heat Sterilization:** Place the materials to be sterilized in a hot oven at 160°C for 2 hours.

**Results:**

*   Observe the agar plates after incubation for any signs of contamination.
*   Record the results and discuss the importance of aseptic technique in preventing contamination.

**Discussion Questions:**

1.  What is the purpose of aseptic technique in microbiology?[/INST]