<s>[INST] A genetic study was performed with daphnia and tested different concentrations of an insecticide. Individuals were crossed according to the following genotype and phenotype table:






|  | genotype | phenotype |
| --- | --- | --- |
| Genotype 1 | r/r | Susceptible |
| Genotype 2 | R/R | Resistant |
| Genotype 3 | R/r | Resistant |



The study was done with 200 daphnia from G0 generation and they were all set up to breed and lay eggs as follows:

*   50 daphnia are R/r and the rest are r/r
*   In G0 generation, the 50 R/r daphnia are mated with 50 r/r individuals to produce G1 generation
*   In the G1 generation, only the resistant daphnia that are R/r are kept while the susceptible daphnia that are r/r are discarded. The resistant offspring are then mated together to produce the G2 generation.
*   In the G2 generation, the process is repeated with only the resistant R/r offspring being kept.
*   The insecticide is applied to the G0, G1, and G2 generations and the following data on mortality rates due to the insecticide were collected.



| Generation | Number of Resistant Daphnia R/r | Susceptible Daphnia r/r | Total | Insecticide mortality |
| --- | --- | --- | --- | --- |
| G0 | 50 | 150 | 200 | 70\% |
| G1 | 25 | 0 | 25 | 50\% |
| G2 | 27 | 0 | 27 | 30\% |



Explain the expected improvement in the insecticide mortality rate of the G3 generation over G2 generation, using the Hardy-Weinberg principle.[/INST]