The allergens were expressed in insect cells using the Bac-to-Bac baculovirus expression system with three different vectors; pFastBac 1, pFastBac-HBM-TOPO (Honey bee melittin secretion signal) and pI-secSUMOstar (Small-ubiquitin-related-modifier which increases the stability and solubility of proteins).
Ofnæmisvakarnir voru tjáðir í skordýrafrumum með Bac-to-Bac baculoveirutjáningarkerfi með þremur mismunandi plasmíðum; pFastBac 1, pFastBac-HBM-TOPO (Honey bee melittin seytiröð) og pI-secSUMOstar (Small-ubiquitin-related-modifier sem á að auka stöðugleika og leysanleika próteina).