V5 tag from pcDNA 3.1/V5-His B vector was ligated into pFastBac HT B. The genes were amplified from cDNA lambda library made from C. nubeculosus salivary glands, ligated into pFastBac HT B-V 5 and pFasBac HT B and sequenced, a 21 is 1218 basepairs (bp) (405 amino acids (aa)) and c 4 is 1254 bp (418 aa).
V5 merkihali úr pcDNA 3.1/V5-His B ferju var settur inn á pFastBac HT B og virkar vel í próteingreiningu. Genin voru mögnuð upp úr cDNA lambdasafni gerðu úr bitkirtlum C. nubeculosus, límd inn í pFastBac HT B-V 5 og pFastBac HT B og síðan raðgreind, a 21 er 1218 basapör (405 amínósýrur) en c 4 1254 basapör (418 amínósýrur).