The effects of the fatty acids were determined by measuring the concentration of tumor necrosis factor-α (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon-γ (IFN-γ), interleukin-17 (IL-17), IL-13, IL-10, CC chemokine ligand 3 (CCL 3) and IL-8/CXC chemokine ligand 8 (CXCL 8) in the culture supernatants by enzyme-linked immunosorbent assay (ELISA).
Áhrif fitusýranna voru ákvörðuð með því að mæla styrk TNF-α, GM-CSF, IFN-γ, IL-17, IL-13, IL-10, CCL 3 og IL-8/CXCL 8 boðefna og flakkboða í frumuflotinu með ELISA aðferð.