Two sets of primers were used, R 3112 and F2987 for W chromosome and R 3112 and F3002 for Z chromosome, therefore two DNA amplifications using PCR were needed for every bird.
Við kyngreiningu voru notuð tvö mismunandi sett þreifara, R 3112 og F2987 fyrir W litning en R 3112 og F3007 fyrir Z litning.