In this part of the study a triple mutation, H119 A/R 120S/R 121G, was carried out on the thermophilic protein aqualysin I. This mutation was chosen by comparison to the amino acid sequence of homologous enzyme from a psychrophilic Vibrio-species (VPR).
Í þessu verkefni var framkvæmd þrefalda stökkbreytingin H119 A/R 120S/R 121G á hitakæra ensíminu aqualysini I. Var þessi stökkbreyting valin með tilliti til samsvarandi amínósýruraðar í samstofna ensími úr kuldakærri Vibrio-tegund (VPR).