The entire fluid lipid content of the gland was isolated with organic solvent, fatty acids were methylestered and the types of fatty acids separated on gas chromatography.
Heildar fituefni stélrótarkirtils var síðan einangrað með lífrænum leysi, fitusýrur fituefnisins metýlesteraðar og tegundir fitusýrumetýlestera greindar á gasgreini.