robert knollys's children included francis knollys, a son henry and two daughters, mary and jane. the latter married sir charles wingfield of kimbolton castle. the son henry (died 1583) was in some favour with edward vi and queen elizabeth. he went abroad with his brother francis during queen mary of england's reign. in 1562 he was sent on a diplomatic mission to germany, to observe the temper of german protestants, and in 1569 was temporarily employed in warding both mary, queen of scots, at tutbury and the duke of norfolk in the tower. he was m.p. for reading in 1563, and for christchurch in 1572. his will, dated 27 july 1582, was proved 2 september 1583.