wide-field multiphoton microscopy refers to an optical non-linear imaging technique tailored for ultrafast imaging in which a large area of the object is illuminated and imaged without the need for scanning. high intensities are required to induce non-linear optical processes such as two-photon fluorescence or second harmonic generation. in scanning multiphoton microscopes the high intensities are achieved by tightly focusing the light, and the image is obtained by stage- or beam-scanning the sample. in wide-field multiphoton microscopy the high intensities are best achieved using an optically amplified pulsed laser source to attain a large field of view (~100 µm). the image in this case is obtained as a single frame with a ccd without the need of scanning, making the technique particularly useful to visualize dynamic processes simultaneously across the object of interest. with wide-field multiphoton microscopy the frame rate can be increased up to a 1000-fold compared to multiphoton scanning microscopy.