all the components of the tramp complex are inter-related to each other. for the activity of poly(a) polymerases liketrf4p/trf5p, zinc knuckle proteins are essential. in similar way rna degradation brought about by exosomes is stimulated by unwinding activity of ski2 like helicases and mtr4p which acts as a cofactor. the unwinding activity of mtr4p is improved by the trf4p/air2p in the tramp complex. mtr4p also has an important role in maintaining and controlling the length of poly(a) tails. but destruction or absence of mtr4p results in hyperadenylation and hinders the length of poly(a) tails.