hollande solution is a version of bouin solution that contains copper acetate. the copper acetate stabilizes red blood cell membranes and the granules of eosinophils and endocrine cells so that there is less lysis of these cell components than occurs in regular bouin solution. hollandes fixative is made by adding 6.25g cupric acetate, 10g picric acid (wet powder), 25ml formalin (37-40% formaldehyde) and 2.5 ml glacial (100%) acetic acid to 250ml water.