robert knollys died in 1521, and was buried in the church of st helen's bishopsgate. his will, dated 13 november 1520, was proved 19 june 1521. his widow, letitia or lettice, was daughter of sir thomas penyston or penystone, of hawridge and marsworth, both in buckinghamshire, and alice bulstrode, and granddaughter of sir richard penystone and margaret herris, and richard bulstrode and alice knyffe. after robert knollys's death she became the second wife of sir robert lee, of burston, buckinghamshire, son of richard lee (died c.1500) and joan saunders (d.1516). sir robert lee, by whom she had issue, died in 1539, when she became the second wife of sir thomas tresham of rushton, northamptonshire, prior (under queen mary of england) of the knights of st. john of jerusalem. her will, dated 28 june 1557, was proved 11 june 1558.