bot1t6 is a positively charged basic structure. basic toxins are usually more potent than acidic ones. in addition, within the buthidae neurotoxin family toxins, a higher total positive charge leads to a more toxic neuropeptide . these characteristics contribute in making bot1t6 a very effective insect toxin. for cockroaches for instance, the ld50 was 10 ng/100 mg. amongst other things this toxin leads to different forms of paralysis and uncoordinated motion. although paralysis can sometimes be reversed but death can also occur without any previous warning symptoms. these effects occur only in insects.