in 1602 marianna gave birth to an illegitimate stillborn baby. after this, the relationship with osio temporarily ended. despite this, after a while the affair restarted, although the lovers' meetings were less frequent. in autumn 1603 sister virginia got pregnant for the second time, and gave birth to a girl. the child was named alma francesca margherita. she subsequently lived with the father, count osio, who legally acknowledged her as his illegitimate daughter two years later in 1605.