despite the result from the "calliphora vicina" experimental study, refrigeration could affect post mortem interval (pmi) calculation because it may cause physiological disturbances in certain insect species. however, this refrigeration process must be done if the insects collected at the crime scene cannot be sent to a forensic entomologist immediately. because insect development could advance after the refrigeration period, it could lead to an underestimation of the time of death.