Title:
Efficient and eco-friendly extraction of corn germ oil using aqueous ethanol solution assisted by steam explosion.

Abstract:
An improved aqueous extraction method has been established for extraction of oil from corn germs. This method primarily included steam explosion pretreatment and aqueous ethanol extraction. Process variables such as steam pressure, resident time, particle size and ethanol concentration were investigated. The highest yield of 93.74 % was obtained when ground steam-exploded corn germ (1.3 MPa, 30 s, 30-35 μm particle size) was treated with 30 % (v/v) aqueous ethanol for 2 h, at 60 °C and pH 9.0. The residual oil content in water and sediment phase decreased dramatically to 4 % and 3 %, respectively. The enhancement mechanism of the process induced by steam explosion was analyzed by confocal laser scanning microscope (CLSM). The quality of extracted crude oil was also investigated. The results showed that the quality of extracted oil was superior to commercial oils.