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  + #### Genetic Toxicology
  + #### Developmental Toxicology
  + #### Investigative Toxicology
  + #### Cytotoxicity
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# ToxTracker Suite
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The ToxTracker suite contains a unique collection of *in vitro* genotoxicity assays that can reliably identify genotoxic compounds and can provide detailed insight into their mode-of-action.  consists of a collection of 6 unique fluorescent reporter cell lines that are combined in a single assay. These reporters signal the activation of various cellular stress responses that indicate the induction of DNA damage, oxidative stress, and protein damage. The extended assay, **,**combines these different fluorescent reporters with an extensive cell cycle analysis and polyploidy analysis to discriminate between genotoxins with a clastogenic or an aneugenic mode-of-action. Aneugens can be further characterised in  to support an Adverse Outcome Pathway (AOP) approach. Genotoxic effects that are indirectly caused by high levels of oxidative stress can be readily identified in . The ToxTracker suite is particularly useful for AOP and read-across approaches. All assays can be useful as stand-alone or as follow-up tests of ToxTracker.
ToxTracker is typically used for early genotoxicity screening and as a follow-up test for mode-of-action assessment. ToxTracker requires small amounts of test material, has a short turn-around time, and has an excellent predictivity for the Ames mutation test, as well as, the *in vitro* and *in vivo* micronucleus assay. The mode-of-action information from the ToxTracker suite can be highly valuable during compound development or in a weight-of-evidence approach for regulatory application.
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ToxTracker is a stem cell-based assay that combines 6 fluorescent reporter cell lines to discriminate between induction of DNA damage, induction of oxidative stress, and protein damage. Their collective induction profile provides mechanistic insight into specific genotoxic properties of compounds.
**Typical applications**
* Identification of genotoxic compounds with high sensitivity and specificity
* High throughput genotoxicity screening
* Investigations on mode-of-action of genotoxic compounds
ToxTracker ACE (Aneugen and Clastogen Evaluation) is an extended version of the ToxTracker assay and includes cell cycle analysis and polyploidy detection in addition to activation of the 6 fluorescent ToxTracker reporters to allow direct discrimination between an aneugenic and clastogenic mode-of-action of genotoxic compounds. ToxTracker ACE is able to separate clastogens from aneugens and identify tubulin poisons and cell cycle kinase inhibitors.
* Genotoxicity testing of pharmaceutical compounds
* Identification of aneugens
* Genotoxicity testing of kinase inhibitors
TubulinTracker is an extension to the collection of fluorescent reporter cell lines in ToxTracker. The TubulinTracker assay is a tubulin-GFP reporter assay that can be applied to visualise microtubule polymerisation dynamics, progression of cells through mitosis, and aneugenicity. TubulinTracker has been developed as a follow-up for the ToxTracker ACE assay to further assess the mode of action of aneugenic compounds by discriminating between tubulin stabilisers and destabilisers.
* Follow-up of ToxTracker (ACE) for compounds with an aneugenic mode-of-action
* Follow-up of a positive *in vitro* micronucleus test
* AOP approach for tubulin poisons
High levels of reactive oxygen species (ROS) and oxidative stress can lead to DNA damage. To investigate the contribution of oxidative stress in the genotoxic profile of a compound, ToxTracker can be performed in presence of a ROS scavenger (NAC and GSH) and measure its impact on DNA damage, oxidative stress, and cytotoxicity. This will allow for the discrimination between direct DNA reactivity of a compound and indirect genotoxicity caused by oxidative stress.
* Genotoxicity testing of compounds when oxidative stress is the expected mode-of-action
* Follow-up of a positive ToxTracker test
ToxPlot is a unique software for automated ToxTracker data analysis and unbiased data interpretation. Graphical representation of the test results allows for a clear and rapid assessment of the reactive properties of compounds. Data analysis is customisable and reports can be easily generated with just the push of a button. ToxPlot is available for every ToxTracker project that is performed by Toxys as a service as well as for projects that are performed in your own lab.
* Data analysis for the ToxTracker assay
* Automated report generation
* Building a database with all your historical ToxTracker results
* Compare test results from different projects and compare them with a library of reference compounds
### More information
If you have any questions on the ToxTracker suite please contact us via info@toxys.com or complete the contact form. Please also see:
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##### About Us
Toxys is a Dutch biotech company that provides innovative in vitro toxicity screening solutions to rapidly identify hazardous and potential carcinogenic properties of novel compounds during the early phases of product and drug development.
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##### Company details
**Toxys Europe
 (Headquarters)**
 De Limes 7
 2342 DH Oegstgeest
 The NetherlandsPhone: +31 71 3322470
 Email: info@toxys.com**Toxys US**
 228 East 45th Street
 Suite 9E
 New York
 New York 10017
 United StatesEmail: info.us@toxys.com
Chamber of commerce: **61077976**
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# ToxTracker ACE
The ToxTracker assay is a state-of-the-art stem cell-based reporter assay that provides mechanistic insight into genotoxic properties of compounds. ToxTracker ACE (Aneugen Clastogen Evaluation) is an extended version of the ToxTracker assay and includes direct discrimination between an aneugenic and a clastogenic mode-of-action of genotoxic compounds. ToxTracker ACE combines the power of 6 fluorescent reporter cell lines for detection of DNA damage, oxidative stress and protein damage with cell cycle analyses and detection of polyploidy. This unique combination gives unparalleled mechanistic insight into the hazardous properties of chemicals and pharmaceuticals in a single assay. The information generated is particularly useful in Mode-of-Action (MoA) and Adverse-Outcome Pathway (AOP) approaches.
**Key Features**
* mechanistic insight into (geno)toxicity
* unsurpassed sensitivity and specificity of 95%
* excellent predictor for regulatory *in vitro*  and *in vivo*  assays
* low amount of compound required
* rapid and cost effective
* metabolic activation using S9 liver extract
ToxTracker ACE is an extended version of the  and includes cell cycle analyses and polyploidy detection to allow direct discrimination between an aneugenic and clastogenic mode-of-action. ToxTracker ACE (Aneugen Clastogen Evaluation) combines the 6 fluorescent reporter cell lines for detection of DNA damage, oxidative stress and protein damage with a DNA staining for cell cycle and chromosome analysis. The combination of differential activation of these endpoints is a unique and very powerful means to separate clastogens from aneugens, as well as to identify potential tubulin poisons and cell cycle kinase inhibitors.
### Unique combination of endpoints
### A graphic of the ToxTracker genotoxicity assay’s panel of GFP-based biomarker reporters and the types of toxicological mode-of-actions they indicate.A graphic of the ToxTracker genotoxicity assay’s panel of GFP-based biomarker reporters and the types of toxicological mode-of-actions they indicate.
### Clastogen vs. Aneugens example
We compared cisplatin (DNA cross linker), taxol (tubulin poison) and AMG900 (kinase inhibitor).
Clastogens:
* Activation of both genotoxicity reporters in ToxTracker
* Cell cycle block in S phase
* No polyploidy
Aneugens/tubulin poisons
* Selective activation of the Rtkn genotoxicity reporter
* Cell cycle block in G2/M phase
* Polyploidy
* Optional: perform 
Aneugens/kinase inhibitors
* No activation of the ToxTracker genotoxicity reporters
* Optional: PhosoH3 staining for Aurora kinase B inhibition
#### From sample to report
This visualisation illustrates the sequence of events between receiving your test compound, performing the ToxTracker ACE and providing the final results.
### Fee-for-Services
We can perform ToxTracker ACE for you at our state-of-the-art laboratory. You can then send your compounds and receive a full report, in most cases within 2 weeks. Most of our customers ask us to perform the assay as a fee-for-service project as we have all tools and equipment in house and are experienced in the data interpretation. We work together on this basis with various of the top 10 pharma, chemical and cosmetics companies.
The following infographic depicts the typical workflow when doing a project with Toxys.
### Local lab installation
The assay can also be installed in your laboratory under license. We provide full support for integration of ToxTracker ACE in your toxicology laboratory. Please contact us via the contact form below or via email: info@toxys.com
### ToxTracker ACE in Japan via Eolas Biosciences
Toxys has partnered with Eolas Biosciences to include ToxTracker ACE in their portfolio of genetic toxicology services for the Japanese Market. Visit  to learn more.
### ToxTracker ACE via research marketplaces Science Exchange and Scientist.com
Toxys has made its ToxTracker ACE assay available through the research service marketplaces  and . These platforms are used worldwide by large and small companies to source their testing services. Organisations listed as buyers can now make use of the standardised legal agreements of these platforms and efficiently order ToxTracker services.
### ToxTracker ACE offered by other CROs
ToxTracker ACE is also available via  in Harrogate (UK).
  Ask a question or get a quote
These are some of the typical application of ToxTracker ACE:
### Meet the study director for ToxTracker ACE
Our study directors are the experts in the field to whom you can ask any question about our assays. From early screening to regulatory safety assessment, our study directors are able to listen to your questions and think along with you to provide a tailored solution. Here are some typical questions they often receive on ToxTracker ACE in understanding where and how ToxTracker ACE can be utilized in their strategies. Please feel free to ask your questions as well.
Can I meet you to talk about my study design?
I would be more than happy to think along with the best study design for your project. Please just reach out to set-up a meeting to discuss the study design.
Click for the answer
Will you also take me through the data after a project in a TC or meeting?
Certainly, once we have shared the draft report and you have had to opportunity to go through the project report, we will schedule a TC to discuss the project with you and answer any questions you might have. You can also always contact me via email or give me a call if you would like to know more.
##### **dr. Inger Brandsma** Study director for ToxTracker suite
Inger Brandsma obtained her PhD from the Erasmus Medical Centre in Rotterdam, where she studied the role of changes in DNA double strand break repair in cancer cells relating to resistance to PARP inhibitors. Inger joined Toxys as a senior scientist, utilising her expertise in DNA damage responses, genome stability and cancer to develop novel mechanistic toxicity assays. Inger was pivotal in developing ToxTracker extensions for ToxTracker ACE, AO and TubulinTracker.
Can you help with a study design for difficult to dissolve compounds?
Of course, we have a lot of experience with a wide range of substances. Before the start of the project, we can discuss the requirements for sample preparation with you and tailor the project to the requirements for your compounds.
Do you also have a question? Click here to send a question to Inger.
#### Send your question
Please fill in the following details to submit your question on ToxTracker. We will get back to you as soon as possible.
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#### Q: Question
Inger:  Do you also have a question? Click here to send a question to Inger.
Please click here for more information on 
If you have any questions on the assay please contact us via info@toxys.com or complete the contact form. Please also see:
  Ask a question or get a quote !