text: F.QUANT, powered by PAIA, is a bead- and plate-based fluorescence assay that measures the concentration of target antibodies in biological samples (e.g. serum, plasma and culture supernatant). The kit contains a bead-filled 384-well plate and a fluorescent reagent mix for fast and direct titer measurement, enabling reliable identification of high-producing cell lines for manufacturing biotherapeutics and high-throughput antibody discovery workflows.
  CYTENA offers two different F.QUANT Titer assay kits. The F.QUANT Fc Titer assay is specific for Fc in IgG and other proteins containing the Fc part of IgG, such as Fc fusion proteins and bispecifics, while the F.QUANT Fab Titer assay recognizes human Fab fragments.
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  Key Features & Benefits
  Mix your sample with the F.QUANT Titer mix in the F.QUANT Titer plate for direct quantification.
  No cell removal
  or wash necessary
  Pipette the supernatant directly into the F.QUANT Titer plate without washing steps needed, given that residual cells do not affect the results.
  Optimized for clone
  screening in CLD
  Use our optimized protocols for monoclonal antibodies.
  Mix, shake and
  Easily measure the titer of your samples in just a few simple steps.
  on liquid handlers
  Set up the F.QUANT Titer assay easily in your automated CLD workflows.
  as little as 5 µL
  Take out a small volume of cell supernatant for measurements and continue with culturing.
  Choose the best
  Monoclonal antibody titer quantification
  Bispecific titer quantification
  Fc fusion protein titer quantification
  Fluorescent titer quantification
  Bead-based titer quantification
  Download Application Notes
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  F.QUANT Fc Titer | CY.FQ.TIT.103 | Excitation 640 nm Emission 665 nm | 10x 384-well F.QUANT Fc Titer Plate and 200 mL Fc Titer Mix | 4ºC |
  F.QUANT Fab Titer | CY.FQ.TIT.104 | Excitation 640 nm Emission 665 nm | 10x 384-well F.QUANT Fab Titer Plate and 200 mL Fab Titer Mix | 4ºC |
  F.QUANT Fc Titer Trial Size | CY.FQ.TIT.101 | Excitation 640 nm Emission 665 nm | 2x 384-well F.QUANT Fc Titer Plate and 2x 25 mL Fc Titer Mix | 4ºC |
  F.QUANT Fab Titer Trial Size | CY.FQ.TIT.102 | Excitation 640 nm Emission 665 nm | 2x 384-well F.QUANT Fab Titer Plate and 2x 25 mL Fab Titer Mix | 4ºC |