The Scots, who top their Euro 2017 qualifying group, host nearest challengers Iceland on Friday evening.
Midfielder Little, 25, recently won the BBC Women's Footballer of the Year award and is likely to play a key role for Signeul at the Falkirk Stadium.
"It is a really well deserved award for Kim," said Signeul, whose team have five wins from five in Group One.
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"Every team in the world would like Kim Little in their team. She is such an ambassador for the game and she is a role model for all young girls in this country.
"She is an amazing player and person. She will be very important for us."
Signeul believes four points from the next two qualifying games will be enough to secure Scotland's place at their first major finals.
After facing Iceland, they will travel to play Belarus on Tuesday. Their final qualifier, away to Iceland, is in September.
Like Signeul's side, Iceland have a 100% record in qualifying, but the Scots have played a game more.
"It is a chance for us to show how good we are, a chance for us to qualify for the Euros," said Signeul.
"We have two games coming up. We need four points to actually qualify. That is what our aim is.
"If we win tomorrow against Iceland, that would be fantastic. However, re-focus, go to Belarus and win there and then we can relax a little bit.
"Iceland are a very strong team. They are physical, tactically very good like their men's team, and technically very good, so it will be a really tough game.
"However, we have a good squad. We have good players, who are also physically very good - maybe not as tall - but who are starting to get taller in the squad.
"I definitely think that we look very good."

The body of Christopher Butler, 27, from Southsea, was discovered at a flat in Waverley Road in the early hours of Friday.
A 23-year-old woman and a 33-year-old man, both from Portsmouth, are being held, Hampshire Constabulary said.
A post-mortem examination on Friday was inconclusive.

Topal, a 29-year-old Turkey midfielder with Fenerbahce, was driving home with a team-mate after training when the car was attacked.
The club condemned the incident as an "armed terrorist act".
The reason for the attack is unclear, but Fenerbahce's team bus was shot at earlier this year and the driver was wounded in the city of Trabzon.
The bus had been driving over a viaduct and a club official was forced to intervene to bring the vehicle to a halt.
The club said at the time that the attack represented the "peak point of hostility" towards it.
Turkey's top-flight Super League was suspended for a week and President Recep Tayyip Erdogan, seen as a Fenerbahce supporter, called a meeting of the league's 18 club captains.
No motive has been established for Tuesday's attack.
BBC Turkish reporter Emre Bal said that although there was an intense rivalry between fans of Fenerbahce and another Istanbul club, Galatasaray, Mehmet Topal was a popular player.
Former Fenerbahce footballer Kennet Andersson told the BBC that opposing fans regularly threw objects including fireworks at team buses, but the latest violence was different. "It sounds much worse now and it shouldn't be like that," he said.
Immediately after the shooting, Topal pulled his car off the road in Istanbul's eastern Sancaktepe district and called for police help.
Topal's team-mate Uygar Mert Zeybek was also unhurt.
Pictures released by the club showed bullet damage on the passenger side of the widescreen, although the bullet did not appear to have pierced the glass.
The club had earlier published pictures on its website of recent signing Robin Van Persie taking part in training.
Fenerbahce were knocked out of the qualifying rounds of the Uefa Champions League by Shakhtar Donetsk last week.
Scotland women's coach Anna Signeul believes any team in the world would want Kim Little in their starting XI.
[NEXT_CONCEPT]
Two people have been arrested on suspicion of murder following the death of a man in Portsmouth on New Year's Day.
[NEXT_CONCEPT]
Turkish footballer Mehmet Topal has escaped unharmed after gunmen fired on his bulletproof car in Istanbul.