Three weeks on from the violence, the bullet holes that pockmarked the windows of La Bonne Biere are gone and Parisians have returned to its terrace.
"It is time for us to gather together again, united, and to go forward in order not to forget," the owners said.
The attacks on 13 November left 130 people dead and more than 350 wounded.
Close-circuit cameras at La Bonne Biere recorded the moment two gunmen approached the cafe and opened fire at those sitting on the terrace.
Piles of flowers and candles still line the pavement where the victims died, but for the first time in three weeks on Friday a handful of customers returned to the tables and chairs outside.
A banner above the entrance reads "Je suis en terrasse" - I am on the terrace - a message of defiance popular among Parisians in the wake of the attacks.
Manager Audrey Bily thanked supporters and said the cafe would "bounce back".
"I would like to thank everyone who has supported us for your poems, your messages and posts that have so helped us," she said.
"We are going to start again, to bounce back. We have carried out some work and repainted the walls to wipe away the signs of this nightmare.
"The Bonne Biere cafe was a place where people meet and exchanged and shared. That is what we want it to be again today."
A handwritten message on a blackboard outside the cafe offered condolences to the victims' families and thanked the emergency services at the scene that night.
Paule Zlotnik, a neighboring shopkeeper, praised the cafe's decision to reopen. "It's time they open and that we continue life as it was before," he said.
The Casa Nostra restaurant opposite the cafe, which was also hit, remains closed. The four other cafes and restaurants targeted in the attacks as well as the nearby Bataclan concert hall, where 89 people died, are also still closed.

New South Wales on Tuesday unveiled changes to its high school syllabus for the first time in 18 years.
The new focus eschews "social context" teaching - which had drawn criticism - for more in-depth, critical thinking.
It comes after an international study in December showed Australia's maths and science ranking had tumbled.
The changes address recent criticism over a lack of rigour, said Tom Alegounarias, the head of the state's Education Standards Authority.
"We reviewed the whole [syllabus], we looked at what the community is demanding for now and in the future," Mr Alegounarias told the Australian Broadcasting Corporation.
"The major shift is towards greater depth, rigour, and mastery of content learning."
One of Australia's leading scientists, quantum physicist Michelle Simmons, recently attacked what she called attempts to "feminise" the teaching of physics.
Prof Simmons said learning by essays rather than formulas, in an apparent attempt to interest more women the subject, was a mistake.
"There is a big cost in this type of thinking," she said in an address last month.
Education officials said these concerns had been addressed, with the new curriculum putting "more science in science" by focusing on its mathematical bases.
Maths will now include more statistics and algorithms, such as those used by internet search engines.
In English, texts will no longer be studied through themes like "journey" or "discovery" but examined for their individual merit.
In history, subjects such as the Enlightenment and the Industrial Revolution will be explored in depth, rather than focusing on a breadth of topics.
The new curriculum will be introduced from next year.
A Paris cafe where five people were killed during the terror attacks last month reopened its doors on Friday - the first of the targets to do so.
[NEXT_CONCEPT]
Australia's most populous state will focus on better preparing students for university and employment in a major overhaul of its school curriculum.