The Edinburgh stands with Orlando event follows the death of 49 people at the gay club pulse.
A total of about 300 people were in the Florida nightclub when the attack started. A further 50 of them were injured
It was the worst mass shooting in US history.
First Minister Nicola Sturgeon, Scottish Tory leader Ruth Davidson and Scottish Labour leader Kezia Dugdale were among those in attending.
Ms Sturgeon said: " "I can't tell you how important it is to me to be with you here this evening. The last few days have been horrific and heartbreaking beyond words but it has also been inspiring.
"All across the world we have seen people come together in solidarity and that is the comfort I hope all of you and members of the LGBTI community across the world take from such expressions.
"We stand here in defiance of hate with a very clear message - love is love."
Ms Davidson said: ""As a woman who moved to Edinburgh to be herself, I know how important LGBTI venues are for people who want to be themselves.
"We stand in Edinburgh in a place where we feel safe and stand in solidarity with people around the world.
"We will become stronger because we have stood here tonight, because we have shown those people that would conduct hate upon our community thay they will not win because love wins, love always win."

Scott, 23, joined the Royals' academy aged eight and helped Kelly Chambers' side maintain their top-flight status last term, following promotion in 2015.
"I am absolutely delighted to have signed a new deal with my childhood club," Scott told the club website.
Manager Chambers added: "Her work ethic and tenacity adds a great dimension to our defence."

In addition to the ban for single women, married women can only freeze their eggs in specific circumstances - for instance when they are to undergo chemotherapy, state media say.
The ban is not new but caught attention after news that actress Xu Jinglei had gone to the US to have her eggs frozen.
Many have ridiculed the ban and described it as sexist.
The debate began in July, after Xu Jinglei, 41, said that she had frozen her ova, or egg cells, in the US in 2013.
The 41-year-old, who is considered one of China's "Big Four" actresses, said she had taken the decision because she might want children in the future.
Then on Sunday, state-run broadcaster CCTV ran a report on the subject, which highlighted some of the risks associated with the procedure and said the ban was "in accordance with family planning policies".
The report triggered a barrage of criticism on Chinese social media, with close to 33,000 comments on CCTV's weibo (microblog) thread alone, and more than 11 million views for hashtags related to the subject.
"We don't even have control over own own ovaries anymore!" user "Kitty is a Lace Lover" wrote.
"Its meaning is that unmarried women don't have the right to reproduce," user Fish girl wrote.
Many questioned why China allowed male sperm donors, but prevented women from freezing their eggs, while others mocked the ban as an attempt to force more women to marry and have children in a hurry.
"This ban should help the men that no one wants!" one user wrote.
There are fewer limitations on the storage of sperm in China.
State media say the restrictions are designed to combat a black market in human eggs, and have stressed the possible health risks associated with the procedure.
China introduced its one-child policy at the end of the 1970s to curb rapid population growth - but eased the policy in 2013 amid concerns over the country's ageing population.
Hundreds of people have gathered in Edinburgh's St Andrew Square to pay their respects to the victims of the mass shooting in Orlando.
[NEXT_CONCEPT]
Reading Women defender Harriet Scott has signed a new deal with the Women's Super League One club until 2018.
[NEXT_CONCEPT]
There has been intense debate in China over a controversial ban on single women freezing their eggs.