Pupils at Denbigh High School were disciplined on the first day of term on Monday, for breaches of policy including "the wearing of inappropriate trousers, skirts and footwear".
The students were taken from their normal lessons and moved to a "learning suite".
About 20 of them returned to normal classes by the end of the day.
Head teacher Simeon Molloy said: "We believe that standards across the board, including uniform, contribute to a positive learning environment.
"At the end of the last school year we held several school assemblies that outlined our school uniform policy.
"We followed this up by telephoning parents and sending out letters with photographs and information on the style of trousers and length of skirts."
He added: "We have spoken with every parent of every student involved in this matter to explain individual issues with each pupil's uniform. We will continue to uphold our high expectations."
Students flouting uniform rules were sent home from lessons at a Kent academy - a day after police intervened in angry scenes at the school gates on the first day of term.

She had been due to attend a rally but instead has been lobbying congress members and party leaders for support.
Latest estimates suggest those in favour of impeachment have just enough votes for the motion to carry.
Ms Rousseff is accused of manipulating government accounts. She says her opponents are mounting a "coup".
Stepping up her bid for survival, she wrote in the Folha de Sao Paulo, (in Portuguese): "They want to convict an innocent woman and save the corrupt," a reference to charges several politicians face.
She accused her opponents of "fraud and lies in an attempt to dismiss a legitimately elected government and replace it with a government without legitimacy".
Denying she had committed any crime, she said: "This is a coup against the republic, against democracy and above all against the votes of all Brazilians who participated in the electoral process."
The lower house of parliament began its debate on impeachment on Friday and a vote is expected on Sunday.
The rowdy session went through the night and has continued on Saturday.
If two-thirds back impeachment, it goes to the Senate, where a simple majority would suspend the president and instigate a trial.
On Tuesday, Ms Rousseff, 68, suggested that Vice-President Michel Temer was one of the ringleaders of the "coup" attempt against her.
She said a widely distributed audio message of Mr Temer appearing to accept replacing her as president was evidence of the conspiracy. However, she did not identify him by name.
Ms Rousseff has also indicated lower house speaker Eduardo Cunha is among those trying to oust her. He is himself facing money-laundering and other charges.
Metal barriers have been built outside parliament in Brasilia to keep rival protesters apart. Protest rallies are also expected in other cities.
513 members of the lower house of Congress
342 votes needed to move process to the Senate
41 senators out of 81 must vote in favour to begin impeachment trial
180 days she could be suspended for during the hearings
Lower house vote: An impeachment vote is due in the lower house on Sunday. A two-thirds majority is required for it to go forward to the Senate.
Senate vote on trial: If Ms Rousseff case is sent to the Senate, a simple majority is enough to suspend her for up to 180 days while she is put on trial. Vice-President Michel Temer would step in during this period.
Impeachment vote: For Ms Rousseff to be removed from office permanently, two-thirds of the Senate would have to vote in favour. Mr Temer would remain president for an interim period should this happen.
A head teacher has defended excluding 72 students from regular classes for not complying with uniform rules.
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Brazilian President Dilma Rousseff has been holding last-minute talks with lawmakers a day before Congress votes on whether to impeach her.