The challenge has seen the 26-year-old sports therapist, from Oswestry in Shropshire, run marathons every day, starting in Chester on 6 August and ending in Manchester on Saturday.
"I feel like I can keep going. If anything I feel stronger," she said.
"If someone suggested running a 54th marathon I probably would to be honest."
That is not to say there have not been some difficult times along the way.
Last week a stomach bug left her vomiting and meant she had to battle through the 26 miles, walking some of the sections.
Overall, however, she said the challenge had been a "fun" experience.
"I was inspired by Larry Macon who ran across the states," Ms Hughes said.
"I didn't really start by wanting to break a record, I just looked at a map of the major cities in the UK and wanting to run in them.
"I've met loads of lovely people and run in some really nice places. Down south, Brighton and Bournemouth were really pretty and the weather was amazing."
In all, Ms Hughes has covered more than 1,300 miles, running in cities such as London, Liverpool, Wolverhampton, Newcastle, Glasgow and Cardiff.
Other runners have joined Amy Hughes along the way and she has had plenty of support, especially on marathon number 50, in her home town of Oswestry, in Shropshire.
"The support was amazing, very emotional. Loads of people came out."
It was also the first time some of her friends and family had seen her since she started the challenge and hundreds of pupils from the Marches School ran a mile with her.
There has also been plenty of backing on Facebook and Twitter, with Paula Radcliffe tweeting her support for Amy's "amazing and inspirational accomplishment".
"Someone sent me a picture the other day, saying that last week I'd already run to Italy, which is crazy," Amy Hughes said.
As well as encouraging children to lead more active lives, Ms Hughes took on the challenge to raise money for the Isabelle Lottie Foundation after a friend's daughter, three-year-old Izzy Wynne, was diagnosed with a form of brain tumour.
She is hoping to raise Â£53,000 for the charity.
Amy Hughes's record still needs to be validated by Guinness World Records before it is official.
In the meantime, she said the only plans she had were "to chill" and take plenty of rest.

Thomas Wainwright was on the wrong side of the road and travelling at speeds of up to 95mph in his Maserati hire car, just minutes before the crash.
The 27-year-old ploughed into the Berlingo van driven by Theresa Wade, 29, on Mull in October 2015.
The court heard Wainwright had been drinking cider and whisky for eight and a half hours before the crash.
He will be sentenced next month.
During the trial Wainwright and his passenger,  42-year-old Jerome Lopez, had claimed that Miss Wade had been on the wrong side of the road and caused the accident but the jury did not believe them.
Witnesses from Mull told how Wainwright, who was with Mr Lopez, his mother's partner, had been drinking at a hotel, a pub and a distillery in Tobermory before having another two pints of cider in the Craignure Inn.
As he headed back towards Tobermory on the wrong side of the road his hire car smashed into the van driven by Miss Wade.
The court heard Wainwright was on Mull, along with members of his family, visiting his sick grandmother. He had flown into Edinburgh airport and hired the Maserati.
Prosecutor Tim Niven-Smith revealed that Wainwright, who worked as the first officer on the Â£6.26m yacht MY Mahogany based in the south of France, had a previous conviction for driving while unfit through drink or drugs.
At a court in Nice he was fined 700 euros and given a suspended sentence.
Judge John Morris remanded Wainwright in custody and banned him from driving.
Finishing her 53rd marathon in as many days, Amy Hughes has run into the record books, not only beating the women's record, but the men's as well.
[NEXT_CONCEPT]
A drink-driver who killed an island vet in a head-on collision has been found guilty of death by dangerous driving.