The woman, known as the Lady of Cao, belonged to the Moche culture in northern Peru and died 1,700 years ago.
Her preserved remains were found in 2006 at a mud-brick ruined pyramid known Huaca Cao Viejo, near Trujillo.
Scientists were able to replicate her face after analysing her skull structure and remains.
The Lady of Cao was buried with a crown, and gold and copper items.
The grave also contained a number of weapons, including two massive war clubs and 23 spear throwers.
A modern autopsy revealed she had been in her 20s when she died, probably of childbirth or pregnancy complications.
Her feet, legs and face were tattooed with magic symbols of serpents and spiders.
The richness of her burial site suggests she might have been a priestess or even a political ruler.
The discovery challenged the belief that only men held high positions in ancient Moche society.
Peru's minister of Culture, Salvador del Solar, said the reconstruction had revealed an oval face with high cheekbones, which many Peruvians shared and would recognise.
"We are privileged to announce this strange combination of the future and the past: technology has allowed us to see the face of a political and religious leader from a culture from the past."
Oval face and high cheekbones - a close-up of the replicated face

It was decided that the surface at the Silverlake Stadium was unsafe during an 08:45 GMT inspection on Tuesday.
Eastleigh must now wait until Saturday's visit of struggling Woking as they look to record their first league win of 2017.
They are 12th in the National League table, eight points adrift of the play-off places and two behind Macclesfield.

"After much soul-searching, it is clearly time for us to live by Michael's words about love not war," wrote Jermaine, in a statement.
Jermaine also withdrew his support for a leaked letter which calls on executors of the estate to resign.
On Thursday his mother, Katherine, was reinstated as guardian of Michael's children along with his cousin, TJ.
Days earlier, TJ became a temporary guardian for the three children amid reports Katherine, 82, was missing.
Michael's siblings, Jermaine, Randy and Janet, are understood to have driven to the Jackson home and insisted they leave with Michael's three children.
Police were called following a "minor scuffle".
"Mistakes have been made and irrational things have been said on both sides in a highly charged emotional environment," Jermaine Jackson wrote in his statement.
"It is time for us all to draw a line in the sand and move towards peace, co-operation, love and healing."
'Mutual suspicions'
Katherine Jackson was on a 10-day spa trip to Arizona, when speculation arose about her whereabouts and her health.
Prince Michael, 15, Paris, 14, and 10-year-old Prince Michael II (also called Blanket) have lived with the singer's mother since his death in 2009.
Lawyers for the estate this week confirmed a number of unnamed relatives have been barred from visiting the Jackson family home.
Writing in his statement about the confrontation at the Jackson family home on 23 July, Jermaine said "it was clear that mutual suspicions had allowed events to spiral out of control".
"I regret that events were ever allowed to reach such a stage," he wrote. "I regret any distress caused to Prince, Paris and Blanket. That was never, ever the intention of myself, Janet, Rebbie or Randy."
Michael Jackson's will left nothing to his siblings when he died aged 50 of an overdose of the anaesthetic propofol in June 2009.
'Overwhelmed'
Janet, Randy, Jermaine and Rebbie are all understood to have signed a letter, which was leaked to the press, alleging the will was a fake and calling on executors of the estate to resign.
The letter states the family was too overwhelmed at the time of the singer's death to meaningfully challenge the will that gave only Katherine Jackson and Michael's three children a stake in the estate.
The estate has denied the accusations, saying they are "saddened" by "false and defamatory accusations".
Any legal challenge to the will should have been filed within four months of the will's acceptance in November 2009.
Scientists in Peru have managed to reconstruct the face of a powerful ancient female leader using 3D printing.
[NEXT_CONCEPT]
Tuesday's National League game between Eastleigh and Macclesfield has been postponed because of a frozen pitch.
[NEXT_CONCEPT]
Michael Jackson's brother Jermaine has called for an end to the Jackson family feud over the late pop star's estate.