Last year meldonium was added to the World Anti-Doping Agency prohibited substance list and several athletes later tested positive for the drug.
Tennis player Maria Sharapova is now serving a two-year ban.
"Athletes and their entourage have ample time to familiarise themselves with the list," Wada president Craig Reedie said.
"There can be no tolerance for people who intentionally break the rules."
The updated list comes into force on 1 January 2017.
It covers substances and methods banned in and out of competition, and which substances are banned in different sports.
Wada said in April that scientists were unsure how long meldonium stayed in an athlete's system.
It also suggested athletes who tested positive before 1 March could avoid bans, provided they had stopped taking it before 1 January.
Sharapova, 29, tested positive for meldonium at the Australian Open in January 2016 and an out-of-competition test on 2 February.
The Russian said she had been taking meldonium since 2006 for health reasons and was unaware it had been added to the banned list as she knew it only by the name mildronate.

Boom Supersonic expects a prototype of its passenger plane to make its first test flight by the end of 2018.
Subsonic and supersonic tests will take place in the US.
If the full-size 55-seat plane is then approved, the first passengers could be travelling at supersonic speeds across the Atlantic by 2023.
Rolls-Royce warns against 'hard Brexit'
Boeing launches new version of 737 jet
Airbus unveils upgraded A380 jumbo
Blake Scholl, the founder and chief executive of Boom, said at the Paris air show that the design of the XB-1 demonstration plane had passed a performance and safety review ahead of manufacturing.
Boom said five unnamed airlines had placed 76 orders for its passenger plane, which resembles Concorde but has a delta wing that sweeps almost to the tip of the nose.
It will also ditch the afterburner engine used by the British-French supersonic pioneer that was not only extremely loud but also very thirsty.
"By using a modern turbofan engine like Boeing and Airbus, you can make the aircraft both quieter and significantly more fuel efficient," Mr Scholl told the BBC.
The company claims that airlines flying its plane will be able to charge similar prices to a business class fare on the lucrative London-New York route of about $5,000 return.
Concorde tickets used to cost up to about three times that amount.
Time-poor travellers, meanwhile, will jump at the chance of getting to or from those two cities in about half the time a subsonic plane takes, according to Boom.
"Airlines are excited for something new and different to offer their passengers," Mr Scholl said.
Although Concorde had the backing of both the British and French governments, he said it was not economic because the imperative then was to beat the Soviet Union into the supersonic age, rather than build a practical or affordable plane.
"Being a private company that has to raise investment means we have to have a business case that is profitable for airlines and affordable for customers," Mr Scholl said.
Although many remain sceptical that Boom can deliver its promises, the company said in March that it had raised the $33m that would allow it to build and fly the demonstration plane.
One believer in the project is Virgin founder Sir Richard Branson, who announced late last year that he planned to buy the first 10 passenger jets made by Boom.
His Virgin Galactic space travel venture will also provide manufacturing and engineering services, as well as flight test support.
Athletes have been warned to prepapre for an updated list of banned substances coming into force in 2017.
[NEXT_CONCEPT]
Supersonic air travel could be back in little more than five years if a plane that aims to replace Concorde takes to the skies.