Two other boys, aged 15 and 16, were assaulted and required treatment.
Police were called to reports of a large number of teenage boys involved in a disturbance at Auchinairn Gardens in Bishopbriggs at about 00:05.
The 16-year-old who was seriously assaulted was treated at Glasgow Royal Infirmary and later released. Police are seeking witnesses to the incident.
Det Sgt Jas Juttla said: "Inquiries are ongoing to establish the full circumstances surrounding this disturbance and I would appeal to any witnesses to come forward."

Dean McIlwaine, from Newtownabbey, was last seen in the Carnmoney Road area of Newtownabbey on Thursday, 13 July.
Hundreds of people joined a search in Belfast on Monday night in a bid to find the 22-year-old barber.
His cousin, Jordan Malone, told the BBC that the family has barely slept since Mr McIlwaine's disappearance five days ago.
He said that he was very close with Mr McIlwaine, as they are both barbers - and that the family cannot understand why he would suddenly go missing.
"Despite the search last night, we've still heard nothing - we're just going up against a brick wall," said Mr Malone.
"Dean and I talked every day, he would never do something like this. Someone would normally know where he is."
The police said Mr McIlwaine was last spotted on the Carnmoney Road on 13 July at about 13:00 BST, wearing a light coloured t-shirt, blue denim shorts and flip-flops.
They have been carrying out searches in the Carnmoney area and across Belfast and renewed their appeal on Tuesday evening.
Hundreds of people, including Mr McIlwaine's father and brother, also took part in a search in Belfast on Monday evening, organised by the Belfast City Council.
Mr Malone said his aunt and uncle have barely slept since their son's disappearance, and are finding it incredibly difficult to cope.
"I think they've had three hours of sleep between them since last Thursday, his brother is like a sheet - he can't take it in," added Mr Malone.
"His mum can't speak. Every time the phone rings or the police arrive, she's afraid it's going to be the worst news."
Mr McIlwaine is described as being 5ft 8 ins tall, of medium build with brown eyes, dark hair and a beard.
He has sleeve tattoos on both arms.
Mr Malone said the family had taken comfort in the support they have received from people since his cousin vanished, and added that they are "so determined" to keep searching for him.
"We'll not give up, we'll keep searching, but as the days go on it does look more negative - there is just no trace of him," he added.
He also had a poignant message for his cousin, in the hope that Mr McIlwaine eventually makes contact with the family.
"I just want to say to him no-one is angry or mad at you. Whatever is going through your head we can help you, we just need to know you're safe.
"We just need to hear from you."
The police have asked anyone who may have seen Mr McIlwaine to contact them on the non-emergency number 101, quoting reference number 121 of 14/7/17.

Police said jewellery was taken from two properties in Barrule Park during the day - taking the total to 32 homes in less than a fortnight.
Insp Derek Flint said 120 incident logs were completed on Tuesday compared to 37 for the same period last year.
Isle of Man residents have been warned by police not to attempt vigilante-style attacks on attempted burglars.
The warning follows what police labelled "wild speculation" on social media.
Insp Flint said "circulating untested information on Facebook puts others at risk and people must stop doing this".
He added: "A call to us with information is much more productive than the wild speculation that some individuals are resorting to on social media.
"Police are working flat out to catch those responsible but that, contrary to speculation, we haven't brought in any officers from the UK.
"This is being managed within our own resources."
Break-ins or attempted break-ins have been reported in Douglas, Onchan, Castletown, Port St Mary, Lonan and Ramsey.
Police are again asking for homeowners to remain vigilant.
A 16-year-old boy has been seriously assaulted in what police said was a "large-scale disturbance" near Glasgow.
[NEXT_CONCEPT]
The family of a missing County Antrim man have said they are determined to "keep searching" until he is found.
[NEXT_CONCEPT]
An unprecedented spate of burglaries on the Isle of Man continued on Tuesday with the first break-ins in Ramsey.