Cooke won the first of GB's gold medals at Beijing in 2008, but since then Lizzie Armitstead has taken over as team leader.
That means Cooke could have to sacrifice gold to Armitstead, with whom she has had differences in the past.
But Cooke said recently: "I know deep down what it takes to win races."
In an interview recorded for Wales at the Olympics, Cooke, who is now based in Switzerland, added: "I have proven through my experience and my results what I'm capable of."
Cooke and Armitstead's relationship was strained at the 2011 World Championships in Copenhagen.
They were in the same GB women's team, with Armitstead the leader.
The team looked well-placed to contend for the title until a crash late in the race separated Armitstead from Cooke up ahead.
They have differing recollections of subsequent events, which saw Cooke set off for the line only to finish fourth, with Armitstead coming home seventh.
Matters came to a head when Armitstead told Cycling Weekly magazine of a furious row once the team had crossed the line, adding: "I've never seen her [Nicole] work for a team-mate. It was a unanimous decision that Nicole didn't do her job properly."
But Cooke says that the pair have now smoothed over their differences ahead of the Olympic road race in London on 29 July.
"Lizzie and I have spoken about what happened in the race [2011 World Championships], what's happened since. I have forgiven for what she did and how she reacted," insisted Cooke.
"And I think in a way it did highlight why we were under-performing as a team.
"We realised that if we went into the London Olympics like we went into the last World Championships we would probably under-perform again."

Cory Schenkel said on Facebook that his grandfather died on Sunday morning in the city of Boise, Idaho.
Kennedy won an Oscar in 1968 for Best Supporting Actor in Cool Hand Luke.
As well as the Naked Gun comedies, he also made memorable appearances in Earthquake and Airport 1975.
Mr Schenkel said the veteran actor had been in poor health since the death of his wife, Joan, more than a year ago, and had been in a hospice for the past month.
Kennedy's daughter, Shannon Kennedy Sullivan, said on Facebook that she hoped people would "remember all the stories he told and the dumb jokes he loved to tell".
"He could always get a laugh and I will miss those but will cherish the little strips of paper he use to write them on when the family would go out to dinner."
Kennedy was born on 18 February 1925 in New York where his father worked as a musician and his mother was a dancer.
He served with the US infantry during World War Two, winning several decorations, and in the 1950s he worked for Armed Forces Radio and Television before moving to Hollywood.
The 6ft 4in actor quickly became well known for playing tough-guy characters.
Mark Wahlberg, the lead in Kennedy's final film, 2014's The Gambler, and Mia Farrow, who starred alongside him in the 1978 film Death On The Nile, were among those who paid tribute to him.
Talk show host Larry King, Family Guy creator Seth McFarlane and actor and comedian Albert Brooks also shared their thoughts.

The two victims, both 15, are being treated in hospital. One remains in a critical condition.
The attack happened at Kingsdale Foundation School at about 15:30 GMT on Thursday.
The teenager accused of attacking the pair has also been charged with grievous bodily harm and possession of an offensive weapon.
He will appear at Camberwell Magistrates' Court on Saturday.
Welsh cyclist Nicole Cooke is in the UK for Sunday's Olympic road race with team orders likely to hinder her chances of another gold medal.
[NEXT_CONCEPT]
US actor George Kennedy, who starred in movies including Cool Hand Luke and the Naked Gun series, has died at the age of 91, his grandson has announced.
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A 15-year-old boy has been charged with attempted murder after two pupils were stabbed at a school in Dulwich.