Ayliffe
Ayliffe  is an English surname. Notable people with this surname include:

Graham Ayliffe (1926–2017), British microbiologist
John Ayliffe (1676–1732), English jurist expelled from Oxford University
Royce Ayliffe (born 1956), Australian professional rugby league footballer
Thomas Hamilton Ayliffe (1774–1852) and family, early settlers of South Australia

See also
Ayliffe Technique, a hand washing technique