OLIGO Primer Analysis Software
OLIGO Primer Analysis Software is a software for DNA primer design. The first paper describing this software was published in 1989. The program is a real time PCR primer and probe search and analysis tool, in addition to siRNA and molecular beacon searches, open reading frame, restriction enzyme analysis. It was created and maintained by Wojciech Rychlik and Piotr Rychlik. OLIGO Primer Analysis Software has been used for: real time PCR, apoptosis studies, antigen typing, species identification, studies on species evolution,  measuring mRNA expression levels, oligonucleotide-based array hybridization studies, degenerate primer studies, microsatellite analysis, DNA microarray detection, inverse PCR, genome walking, nucleotide polymorphisms studies, detection of microorganisms or viruses, genotyping,  cloning, vector (gene) construction, genome sequencing, detection of mutants or intraspecific variability, genetic disease studies, siRNA and gene silencing, FISH analysis (single cell expression study), scorpion probes, and development of new DNA amplification methods. References

Oligo Primer Analysis Software by Molecular Biology Insights

Other Primer Design Software 
 FastPCR
 Gene Designer 
 Geneious
 MacVector
 Netprimer
 Primer Premier
 UGENE
 Vector NTI

Bioinformatics software