Pulchrocladia retipora
Pulchrocladia retipora, commonly known as the coral lichen, is a species of fruticose lichen in the family Cladoniaceae. Found predominantly in Australasia, its habitats range from the Australian Capital Territory to New Zealand's North and South Islands, and even the Pacific region of New Caledonia, where it grows in coastal and alpine heathlands. The lichen features coral-like branches and subbranches with numerous intricate, netlike perforations. It is known by multiple names, with some sources referring to it by its synonym Cladia retipora, or the common name lace lichen. The lichen was first scientifically collected by the French biologist Jacques Labillardière on board the Bruni d'Entrecasteaux expeditions in 1792. Labillardière had erroneously classified it as an alga rather than a lichen. Pulchrocladia retipora was the first Australian lichen to be described in a scientific publication. It is now the type species of the genus Pulchrocladia, created in 2018 following a molecular phylogenetics-led restructuring of the Cladoniaceae. Taxonomy

The species was first formally described by Jacques Labillardière in 1806, as Baeomyces reteporus. The type specimen was collected from Tasmania. This collection was made as part of the botanical collections he made during Labillardière's 1791 voyage to the South Seas with the French explorer Antoine Bruni d'Entrecasteaux in an unsuccessful search for the French naval officer Jean-François de Galaup, comte de Lapérouse. Labillardière had first collected the lichen in 1792, and published the description in Novae Hollandiae Plantarum Specimen, erroneously describing the lichen as an alga. According to the Australian lichenologist Rex Filson, the lichen is the first that was described for Australia. In its taxonomic history, the taxon has been shuffled to several genera, some of which are no longer used or have been synonymized with other genera. In 1814, the Swedish botanist Erik Acharius changed the spelling of the specific epithet to retipora (from retepora) when he introduced the new combination Cenomyce retipora. There was debate on whether Acharius's change was intentional, as he still referred to the basionym as Baeomyces retiporus Labill. The term rete translates to "net", and the correct connecting vowel in such compounds is "i", making retiporus the appropriate spelling. Without evidence that Labillardière deliberately chose the original spelling, it is thought to be an orthographical variant. Since retipora is widely recognized and reverting to the original might cause confusion, it is considered best to maintain the current spelling. Other genera that have housed the species are Pycnothelia (Fée, 1825), Cladonia (Fries, 1826), Cladina (Nylander, 1868), Cladia (Nylander, 1876), and Clathrina (Johann Müller, 1883). Nylander proposed the genus Cladia in 1870 to contain three Cladonia species based on their densely  longitudinally aligned filaments of the cortex. Despite some controversy regarding the use of the name Cladia, and the Swiss botanist Johannes Müller Argoviensis' proposed replacement Clathrina in 1883, Nylander persisted with the genus name Cladia, a decision largely accepted by later authors; Cladia retiporus was the most prevalent name for the lichen for more than a century. It is now the type species of genus Pulchrocladia, which was circumscribed in 2018 by Soili Stenroos, Raquel Pino-Bodas, Helge Thorsten Lumbsch, and Teuvo Ahti following a large-scale molecular phylogenetic analysis of the family Cladoniaceae. It is commonly known as the coral lichen, or the lace lichen. Description

Like most species in the family Cladoniaceae, Pulchrocladia retipora has a  growth form, meaning it has both a primary (horizontal) and secondary (vertical) thallus. The primary thallus is nodular (i.e. with small raised areas or swellings) and white, and only lasts for a short period. are the  that originate from the primary thallus. The podetia are typically up to  tall, white to pale grey in colour, sometimes tinged pinkish or yellowing or superficially blackening at tips. They are rigid when dry, but become spongy when wet. They are irregularly or dichotomously branched, forming cushiony clumps. The wall is highly perforated (about 5–11 perforations per centimetre), with large, round to ellipsoidal holes. The surface of the podetium is continuously  and lacks soredia. The inner medulla is made of twisted strands of hyphae with a cobweb-like form. The lichen, nonetheless, generates soredia-like clusters of algal cells and hyphae within the strands of the inner medulla. Apothecia occur infrequently. When present, they are small, black, , and crowd together at the ends of small, terminal branches (). The colour of the hymenium ranges from dark reddish-brown to black. Ascospores have dimensions of 25–27 by 5 μm. The conidiomata are terminal on branchlets, and covered with translucent slime; they produce curved or straight conidia measuring 6 by 1 μm. Specimens collected in Australia tend to have a brownish tinge, while New Zealand specimens range from pure white to grey, to grayish-green, or slightly yellowish. The photobiont partner is green algae from the Trebouxia. Occasionally, free-living algae become trapped in the irregularly intertwined hyphae of the medulla. Usnic acid and atranorin are the major secondary compounds present in Pulchrocladia retipora. Other compounds occurring in lesser quantity, and detectable using thin-layer chromatography, include protolichesterinic and ursolic acids, and, often also rangiformic acid and norrangiformic acids. It is usnic acid that is thought to be responsible for the antimicrobial, antiviral and cytotoxic biological activity of Pulchrocladia retipora lichen extracts tested in in vitro experiments. The hue of P. retipora is determined by the concentration of usnic acid in the thallus, leading to a colour spectrum that ranges from an opaque greyish-white through yellowish-white to a distinct yellow. Pulchrocladia retipora and P. corallaizon are closely related species, and their similarities may lead to confusion in identifying them. However, one can distinguish P. corallaizon from P. retipora by observing the inner-medulla of the former species, which is characteristically tightly packed. In older pseudopodetia of C. corallaizon, the inner medulla might be absent in places or appear sparse. Nonetheless, it always remains compacted in the top branches of the pseudopodetia, ensuring it never appears stranded or corticated. Habitat and distribution

Pulchrocladia retipora is widely distributed in Australasia. In Australia, it has been recorded from the Australian Capital Territory, New South Wales, Queensland, Victoria, and Tasmania. In New Zealand, it is known from both the North and South Islands as well as the Antipodes Islands, Auckland Islands, Campbell Island, and Chatham Islands. In the Pacific, it occurs in New Caledonia. The coral lichen is particularly common in subalpine peat bogs; it is often found in association with the lichens Cladonia confusa, Rexiella sullivani, and Stereocaulon ramulosum. It is found on peaty soils, found among tussocks or in heaths comprising Dracophyllum and Leptospermum, at the margins of Nothofagus forests, in fellfield, or rarely on surfaces such as rocks, logs, and sand dunes. In the moorlands of the Meredith Range area in Tasmania, it thrives in well-drained, elevated locations, especially close to decomposing buttongrass hummocks. The lichen replicates vegetatively when new podetia grow from fragments of old podetia. It has highly variable growth rates, ranging from less than 1 mm per year to up to a few centimetres per year. It has been noted to grow in clusters, sometimes up a metre in diameter. The unique morphology of the lichen helps it to survive the exposed heaths it inhabits, as the coral structure increases gas exchange, moderates temperature extremes, and maximises light and water access. Pulchrocladia retipora grows in cushion-like growths ranging in diameter from about  to . The New Zealand botanist William Martin remarked finding square metre-sized cushions in the area of Lewis Pass in Canterbury, New Zealand. A separate description describes cushions the size of a football in Australia's Grampians National Park. According to Martin, coral lichen forming massive cushion growths only occur in subalpine zones, with lowland forms being only  in size. Uses and research

The complex net-like structures of Pulchrocladia retipora are known as fenestrations. Rosmarie Honegger referred to the thallus of this species as "likely to be among the most complex vegetative structures ever produced in the fungal kingdom". The appearance of the lichen has been described as "of considerable beauty resembling lace or coral". As a result, it has been used in floral decoration and architectural design. In a discussion of the cover designs of the journal The Lichenologist, the German lichenologist Robert Lücking describes "one of the most striking volume layouts" as one that combined an image of Pulchrocladia retipora with a blue-themed design (volume 37, part 1, 2005). Thallus development
The development and growth dynamics of the branching pattern of Pulchrocladia retipora has been studied. The first structure to emerge from the primary thallus is a meristem, a solid bundle of tissue comprising only fungal cells. Two adjacent meristem bundles give rise to the erect secondary thallus, the podetium. These bundles continue to split , resulting in groups of three meristem bundles. Because the development of same-age bundles is unequal, the developmental differences become more pronounced as the meristem bundles grow farther apart. Splitting of the meristem is not synchronized between approximately same-age bundles; consequently, one meristem bundle or one side of the podetium grows beyond the others. The consequently slight bends and twists of the meristem that occur during early development are later made apparent by the relative angles of the meristem bundles with respect to each other. Perforations that are developmentally unrelated to the central perforation tend to occur early in the lichenised tissue distal to the meristem, while perforations that occur between meristem bundles happen later in development. Resynthesis
The Pulchrocladia retipora lichen thallus has been successfully resynthesized from isolated mycobiont and photobiont under laboratory conditions. In these experiments, lichen primordia, consisting of fungal mycelia and enclosed algae, appear after about one month. After four months, the cultures form minute scales that are the starting units for the development of the complex hyphal network that becomes the thallus. Later, the scales differentiate into columns that grow together vertically to form a thin network, and more hyphae join and fuse together to strengthen and stabilize the network. Eventually, algae colonise the network prior to further development of the inner medulla and connecting of the fenestrations. In the laboratory, the entire process takes about two years. Biomonitoring

The  Baseline Air Pollution Station in Tasmania (part of the World Meteorological Organization-Global Atmosphere Watch network) has used Pulchrocladia retipora as a bioindicator to identify how atmospheric nitrogen and sulphur deposition in Tasmania is affected by human pollution. References

Cited literature

External links
Herbarium records of Pulchrocladia retipora at the Consortium of North American Lichen Herbaria

Cladoniaceae
Lichen species
Lichens described in 1806
Lichens of Australia
Lichens of New Caledonia
Lichens of New Zealand
Taxa named by Jacques Labillardière