Charles Best
Charles Best may refer to:

 Charles Best (poet) (1570–1627), English poet
 Charles Best (British Army officer) (1765–1836), British army officer
 Charles Best (American football) (1874–1962), American college football and basketball coach
 Charles Best (medical scientist) (1899–1978), American-Canadian medical scientist and co-discoverer of insulin
 Charles Best (politician) (1909–1996), Australian politician
 Charles Best (businessman), American businessman and founder of Donorschoose.org
 Charles Alexander Best (1931–1978), Canadian politician, son of Charles Herbert Best