That could possibly explain some results, but not those involving transmission. If the knocked-in DNA is transmitted to the next generation then I&#x27;d think it must have gotten incorporated somewhere, however, this need not be at the intended site if the primers are amplifying the template.<p>Then again, supposedly shingles is caused by extragenomic Varicella-zoster DNA that is somehow stable for decades and can be passed on during pregnancy. I&#x27;m not sure I believe that though, and of course that is viral DNA.<p>Anyway, in that Ruan et al (2015) they claim to have detected exactly the expected sequence across the junction in at least a few cells. I can&#x27;t think of any explanation for that data other than CRISPR working as advertised. However, they don&#x27;t report in what percent of the cells this was observed.<p>Edit:
I mean supposedly those exact sequences shown in figure S2 never physically existed before and now they do, exactly as predicted by the theory. That is strong evidence.