Methylene blue improves Complex IV function in mitochondria, so it certainly has a direct effect on mitochondrial function.  It&#x27;s actually used as an antidote to several poisons that inhibit mitochondrial function[1].<p>[1] <a href="https:&#x2F;&#x2F;en.wikipedia.org&#x2F;wiki&#x2F;Methylene_blue" rel="nofollow">https:&#x2F;&#x2F;en.wikipedia.org&#x2F;wiki&#x2F;Methylene_blue</a>