Cryo Electron Microscopy (Cryo-EM)
Cryogenic electron microscopy
Cryogenic electron microscopy (cryoEM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution." Nature Methods also named cryoEM as the "Method of the Year" in 2015.Read more about 'Cryogenic electron microscopy' at: Wikipedia
Wikipedia contributors. "Cryogenic electron microscopy." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, Feb. 19, 2024.
Other modalities related to Cryo Electron Microscopy in Helmholtz Imaging CONNECT:
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm …
In transmission electron microscopy an electron beam is going through a very thin sample (usually < 100 nm thick). Due to the shorter wavelength of the electrons a far higher resolution can be obtained as …