anchor: If the acclimation process is repeated with a new group of test organisms and excessive mortality occurs, it is recommended that an alternative source of dilution water be used.
positive: If the acclimation process repeats with a new group of organisms and mortality goes up, we should use different water to dilute.
negative: If the acclimation process repeats with a new group of organisms and mortality goes up, we should use the same water to dilute to see if it repeats.