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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Polymerase chain reaction (PCR) is an extra-sensitive test that measures the presence of certain biomarkers in blood or bone marrow cells. It measures any remaining blood cancer cells not found by cytogenetic methods such as FISH. PCR is used to diagnose and check a patient's molecular response to treatment.