Differential centrifugation. Fractionation is a separation process in which a certain quantity of a mixture (gas, solid, liquid, suspension or isotope) is divided during a phase transition, into a number of smaller quantities (fractions) in which the composition varies according to a gradient.lood fractionation refers generally to the process of separation using a centrifuge (centrifugation), after which three major components can be visualized: plasma, buffy coat and erythrocytes.
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