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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Phenylthiocarbamide (PTC) is an organosulfur that has a unique property in that it either tastes very bitter or is tasteless to humans. The gene for this PTC receptor is TAS2R38, which was discovered in 2003.One of the factors that can affect to the ability to taste PTC, or not to taste PTC, is a Single Nucleotide Polymorphism (SNP).CR is a simple and inexpensive tool that is used to amplify a very small amount of DNA. The exponential powers of PCR allow it to produce a very large amount of product that can be analyzed. A sample of my own DNA (cheek cells via saline mouthwash) was used to analyze what type of SNP I have on the TAS2R38 gene.