Bacterial skin infections in dogs often result from an underlying immune disorder. The most common predisposing causes include allergies (inhalant/contact, food, and/or flea bite).acterial skin infections often occur in dogs secondarily to underlying skin allergies, hormonal (endocrine) disorders, or other internal disease. Through a combination of shampoo therapy and antibiotics, the prognosis for your dog's recovery from a bacterial skin infection is excellent.
Clinical chemistry analyzers run assays on clinical samples such as blood serum, plasma, urine, and cerebrospinal fluid to detect the presence of analytes relating to disease or drugs. Clinical chemistry analyzers are used in a variety of settings, including small clinics, research labs, and high-throughput hospital labs. They are also used at the point-of-care, such as in physiciansâ offices and patient bedsides.
The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assesing protein concentrations for gel electrophoresis. Assay materials including color reagent, protein standard, and instruction booklet are available from Bio-Rad Corporation. The method described below is for a 100 Âµl sample volume using 5 ml color reagent. It is sensitive to about 5 to 200 micrograms protein, depending on the dye quality. In assays using 5 ml color reagent prepared in lab, the sensitive range is closer to 5 to 100 Âµg protein. Scale down the volume for the microassay procedure, which uses 1 ml cuvettes. Protocols, including use of microtiter plates are described in the flyer that comes with the Bio-Rad kit.