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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Reaction quotient. In chemistry, a reaction quotient (Qr) is a function of the activities or concentrations of the chemical species involved in a chemical reaction. In the special case that the reaction is at equilibrium the reaction quotient is constant and equal to the equilibrium constant that appears in the expression of the law of mass action. A general chemical reaction in which Î± moles of a reactant A and Î² moles of a reactant B react to give Ï moles of a product R and Ï moles of a product S can be written as