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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
A quantitative trait locus (QTL) is a section of DNA (the locus) that correlates with variation in a phenotype (the quantitative trait).The QTL typically is linked to, or contains, the genes that control that phenotype.QTLs are mapped by identifying which molecular markers (such as SNPs or AFLPs) correlate with an observed trait. This is often an early step in identifying and sequencing the actual genes that cause the trait variation.he principle for QTL mapping is: 1) The Likelihood can be calculated for a given set of parameters (particularly QTL effect and QTL position) given the observed data on phenotypes and marker genotypes. 2) The estimates for the parameters are those where the likelihood are highest.