To take out the useful parts of the food, the digestive system breaks the food down into small pieces. This process is called digestion. The parts of the food which are not useful just pass through the body.
The interrogation. Interrogations consist of three pulses, 0.8 Î¼s in duration, referred to as P1, P2 and P3. The timing between pulses P1 and P3 determines the mode (or question) of the interrogation, and thus what the nature of the reply should be. P2 is used in side-lobe suppression, explained later.
Cultures are then treated with fresh media containing DAPT at the desired range of concentrations for an additional 24 hours at 37 Â°C, and conditioned media collected. For the measurement of total AÎ², samples are analyzed with the same ELISA (266â3D6) as used for the HEK 293 cell assays.