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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
The polymerase chain reaction (PCR) is an enzymatic process that allows for the. detection of specific genes within an environmental DNA sample. PCR utilizes. short, user defined DNA sequences called oligonucleotide primers, the sequence. of which are complementary to target regions of genes known to encode for.