All full-time employees except casuals are entitled to a minimum of 10 days paid personal leave each year (which includes sick leave and carer's leave).This is accrued progressively for a full-time employee and pro-rata for a part-timer.ow much personal leave (sick leave) are my employees entitled to? Under the National Employment Standards (NES), all full-time employees except casuals are entitled to a minimum of 10 days paid personal leave annually (which includes sick leave and carer's leave).
AFLP-PCR or just AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering.FLP not only has higher reproducibility, resolution, and sensitivity at the whole genome level compared to other techniques, but it also has the capability to amplify between 50 and 100 fragments at one time. In addition, no prior sequence information is needed for amplification (Meudt & Clarke 2007).
are differences in restriction fragment lengths caused by SNPs or INDELs that create or abolish restriction endonuclease recognition sites. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA.eaknesses of AFLP. 1  Proprietary technology is needed to score heterozygotes and ++ homozygotes. 2  Developing locus-specific markers from individual fragments can be difficult. 3  Need to use different kits adapted to the size of the genome being analyzed.