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Base pair. Depiction of the adenine-thymine Watson-Crick base pair. A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds. They form the building blocks of the DNA double helix, and contribute to the folded structure of both DNA and RNA.
A set of five nitrogenous bases is used in the construction of nucleotides, which in turn build up the nucleic acids like DNA and RNA. These nitrogenous bases are adenine (A), uracil (U), guanine (G), thymine (T), and cytosine (C). These nitrogenous bases hydrogen bond between opposing DNA strands to form the rungs of the twisted ladder or double helix of DNA or a biological catalyst that is found in the nucleotides. Adenine is always paired with thymine, and guanine is always paired with cytosine. These are known as base pairs.