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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Polymerase chain reaction (PCR) is a method that allows exponential amplification of short DNA sequences. (usually 100 to 600 bases) within a longer double stranded DNA molecule. PCR entails the use of a pair of. primers, each about 20 nucleotides in length, that are complementary to a defined sequence on each of the. two strands of the DNA. These primers are extended by a DNA polymerase so that a copy is made of the.