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1 Breast stimulation can modestly increase the amount of prolactin in the blood. 2  Chest wall trauma (for example, an injury from a car steering wheel after an accident) can lead to increased levels of prolactin.  Marijuana use is also a well documented cause of elevated levels of prolactin.
Hyperprolactinemia, where prolactin levels are higher than normal, leads to suppression of libido and to sexual dysfunction in both men and women. We interpret this effect of high prolactin levels as similar to the refractory period following sex.