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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
In the latter context, focused copy number screening methods are preferentially used, such as MLPA, targeted microarrays, and qPCR. With real-time qPCR, the PCR product accumulation is mea-. suredin real-timeresultingin a sigmoidal ampliï¬cation curve.Sev-. eral detection chemistries are available to measure product.