The Kilcher Homestead is a 600 acre Pioneer Homestead that has been protected for perpetuity via a ConservatIon Easement. The Kilcher Family has also preserved the Historic Cabin and site as a LIving Museum.
Yes, negative pH is possible, but not in water as a solvent. The range of pH in water is 0 to 14 (to be more precise, it is -1.7 to 15.7, but for simplicity's sake, we start with the usual values.) Thus, what pH indicates is the ability of the acid to form H3O+, which can then donate a proton.
As the protein moves to a lower pH region, its surface charge will become less negative, and a pH region will be reached at which the protein net charge is zero (the isoelectric point). The protein will stop moving and, because different proteins have different isoelectric points, separation can be achieved.