Actinic keratoses that develop on the lips are referred to as actinic cheilitis. The clinical appearance of an actinic keratosis is that of a small (several millimeters), red scaly papule or plaque, with induration and thickening of the epidermis.
1 Location: Ideally, the probe should be in close proximity to the forward or reverse primer, but should not overlap with a primer-binding site on the same strand. 2  Probes can be designed to bind to either strand of the target. 3  Melting temperature (Tm): Preferably, probes should have a Tm 6â8Â°C higher than the primers.
Generally, primers should have 18-20 nucleotides of overlap with template stands. 5â primer (forward primer) should align to the first 18-20 nucleotides of the sense strand of the gene ('normal' strand, complement to antisense strand).It should also include the recognition site of the desired restriction enzyme at the 5' end (upstream) of the first nucleotide of overlap.he primer should also include the recognition site of the desired restriction enzyme at the 3' end (downstream) of the last nucleotide of overlap and downstream of the stop codon. Take the reverse-complement of the sequence to make it align to the anti-sense strand.