Actinic keratoses that develop on the lips are referred to as actinic cheilitis. The clinical appearance of an actinic keratosis is that of a small (several millimeters), red scaly papule or plaque, with induration and thickening of the epidermis.
1 Location: Ideally, the probe should be in close proximity to the forward or reverse primer, but should not overlap with a primer-binding site on the same strand. 2  Probes can be designed to bind to either strand of the target. 3  Melting temperature (Tm): Preferably, probes should have a Tm 6â8Â°C higher than the primers.
Best Answer: If you don't reverse the reverse primer, DNA synthesis occurs in the same direction from two positions on the same DNA strand.You wouldn't get amplification because the increase in copy numbers would be arithmetical, not exponential-too little to be seen in an agarose gel.NA is double stranded and complementary. If you do not reverse your primers you will not get both strands being amplified therefore the PCR will not work. Hard to imagine unless you draw it out. quite logical really.