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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
Agar is a gelatinous medium that provides nutrients and a stable, controlled environment for bacteria growth. Most bacteria will grow well using nutrient agar, but some more fastidious bacteria prefer tryptic soy agar. To prepare agar plates, just heat agar and pour it into a sterile petri dish. Swab along any surface to test (your mouth, the bathroom counter, etc.) and then lightly smear in a zig-zag pattern on the surface of the agar plates. Signs of bacteria growth should show within 1-2 weeks. Getting started: