Baking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.aking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.
Quick Answer. Ethanol is used in DNA extraction to force the DNA to precipitate in a solution. In order to collect a DNA sample, cells are broken down through agitation, then mixed with water, salt and ethanol to create an aqueous solution.
The protocol involves lysis of yeast colonies or cells from liquid culture in a lithium acetate (LiOAc)âSDS solution and subsequent precipitation of DNA with ethanol. Approximately 100 nanograms of total genomic DNA can be extracted from 1 Ã 10 7 cells. DNA extracted by this method is suitable for a variety of PCR-based applications (including colony PCR, real-time qPCR, and DNA sequencing) for amplification of DNA fragments of â¤ 3500 bp.