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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
Agar is the jelly-like substance used to culture bacteria. It is made from a type of red algae which provides an ideal growing surface for many different types of bacteria. Some types of agar contain added nutrients (such as sheep's blood) which help to promote more vigorous bacterial growth. In a heat proof dish or bowl, stir half a teaspoon of the nutrient agar powder into 60 ml (approximately 1/4 cup) of hot water. 2  Multiply these quantities for however many petri dishes you plan on using.