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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
An agar plate or Petri dish is used to provide a growth medium using a mix of agar and other nutrients in which microorganisms, including bacteria and fungi, can be cultured and observed under the microscope.he gelling agent in agar is an unbranched polysaccharide obtained from the cell walls of some species of red algae, primarily from the genera Gelidium and Gracilaria.