Baking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.aking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.
Quick Answer. Ethanol is used in DNA extraction to force the DNA to precipitate in a solution. In order to collect a DNA sample, cells are broken down through agitation, then mixed with water, salt and ethanol to create an aqueous solution.
1 DNA purification from detergents, proteins, salts and reagents used during cell lysis step. 2  The most commonly used procedures are: 3  Ethanol precipitation usually by ice-cold ethanol or isopropanol. 4  Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation. Breaking the cells open, commonly referred to as cell disruption or cell lysis, to expose the DNA within. 2  This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample. 3  Removing membrane lipids by adding a detergent or surfactants which also serves in cell lysis.