However, use of the gal is deprecated by ISO 80000-3:2006. The gal is a derived unit, defined in terms of the centimeterâgramâsecond (CGS) base unit of length, the centimeter, and the second, which is the base unit of time in both the CGS and the modern SI system.
It has been shown experimentally that if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will increase until it reaches a maximum. After this point, increases in substrate concentration will not increase the velocity (delta A/delta T). This is represented graphically in Figure 8. It is theorized that when this maximum velocity had been reached, all of the available enzyme has been converted to ES, the enzyme substrate complex.
Effect of Substrate Concentration on Enzyme Activity. 1  Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. 2  Explain why denatured sucrase was used as a control. 3  Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain how increasing amount of sucrose in food affects sucrase activity. 2  In the results section you were asked if rate of increase of sucrase activity was greater when sucrose concentration went from 2.5 to 7.5 g/l or when it went from 22.5 to 27.5 g/l. 3  Explain why the rate of increase was greater for this range.