Baking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.aking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.
Quick Answer. Ethanol is used in DNA extraction to force the DNA to precipitate in a solution. In order to collect a DNA sample, cells are broken down through agitation, then mixed with water, salt and ethanol to create an aqueous solution.
DNA concentration can be determined measuring the intensity of absorbance of the solution at the 600 nm with a spectrophotometer and comparing to a standard curve of known DNA concentrations. Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm is used as a measure of DNA purity. DNA purification from detergents, proteins, salts and reagents used during cell lysis step. 2  The most commonly used procedures are: 3  Ethanol precipitation usually by ice-cold ethanol or isopropanol. 4  Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation.