Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
From west to east, theâ¦y are: Pacific Time, Mountain Time, Central Time, and Eastern Time. Each is one hour later than the last. For example, if it's 3 PM Pacific Time, then it's 4 PM Mountain Time, 5 PM Central Time, and 6 PM Eastern Time.