If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Sponsored Topics. Excelsior is a settlement on Lake Minnetonka in Hennepin County, Minnesota, United States. The population was 2,188 at the 2010 census. According to the United States Census Bureau, the city has a total area of 0.7 square miles (1.7 kmÂ²), of which, 0.6 square miles (1.6 kmÂ²) of it is land and 0.1 square miles (0.1 kmÂ²) of it (7.46%) is water.