Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
Mountain Standard Time (MST) or UTC/GMT -7. The Mountain Time Zone refers to time zone which observes time where seven hours are subtracted from GMT (UTC/GMT -7). This is called Mountain Standard Time (MST). The clock time in this zone is based on the mean solar time of the 105th meridian west of the Greenwich Observatory. Mountain Daylight Time (MDT) refers to time where six hours are subtracted from GMT (UTC/GMT -6). This time is used during the daylight saving time, which is observed throughout the sunnier months of the year, during the spring, summer and autumn. Mountain Time (MT)