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Eosinophils can be detected in the urine by applying stains that attach to their cytoplasmic granules. The three most commonly used stains are the Wright's, Giemsa, and Hansel's stains, and the last is the most effective at staining eosinophils in the urine [6,7]. The presence of more than 1 percent eosinophils of the total white cells in the urine is considered a positive test by any of these three stains.
Below is a brief description of the stains used for the slides in your histology set. A discussion of stains, dyes and histochemistry is also found in your textbook. 1) Hematoxylin and Eosin (H&E: most slides; [Example; Example]) This is a good general stain and is widely used.