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Ethylene Oxide sterilization is a chemical process consisting of four primary variables: gas concentration, humidity, temperature and time. Ethylene oxide is an alkylating agent that disrupts the DNA of microorganisms, which prevents them from reproducing.
Until 1995, ethylene oxide sterilizers combined ETO with a chloroflourocarbon (CFC) stabilizing agent, most commonly in a ratio of 12% ETO mixed with 88% CFC (referred to as 12/88 ETO). For several reasons, healthcare personnel have been exploring the use of new low-temperature sterilization technologies 825, 851.