If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Staples is a city in Todd and Wadena counties in the U.S. state of Minnesota. The population was 2,981 at the 2010 census. According to the United States Census Bureau, the city has a total area of 4.6 square miles (11.8 kmÂ²), of which, 4.5 square miles (11.7 kmÂ²) of it is land and 0.22% is water. U.S. Route 10 and Minnesota State Highway 210 are two of the main routes in the city. The Empire Builder, an Amtrak route connecting Chicago, Illinois with Seattle, Washington and Portland, Oregon, stops at the train station in Staples.