Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
In the United States and Canada, the Mountain Time Zone is one hour ahead of the Pacific Time Zone and one hour behind the Central Time Zone. In some areas, starting in 2007, the local time changes from MST to MDT at 2 am MST to 3 am MDT on the second Sunday in March and returns at 2 am MDT to 1 am MST on the first Sunday in November.