If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Parlier is located 5.5 miles (9 km) east-southeast of Selma, at an elevation of 344 feet (105 m). Parlier is located at 36Â°36'42 North, 119Â°31'37 West. According to the United States Census Bureau, the city has a total area of 2.2 square miles (5.7 kmÂ²), of which, 1.6 square miles (4.2 kmÂ²) of it is land and none of it is covered by water.