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The experiment aims to clarify the meaning of purity in the chemical sense ; apply the method of recrystallization in the purification of a solid sample; recognize melting point as a physical property that can serve as an index of purity; determine the melting point of a substance using a simple apparatus; and compare the melting point of a pure versus impure sample of a substance.
Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.