If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Rebecca Spice, a tourist from Milwaukee, Wisconsin, is flanked by Joel Kopischke, left, and Robert Simonson, both of New York, as they read a bulletin edition of the New York Post reporting the outbreak of hostilities in the Persian Gulf in New Yorkâs Times Square on January 17, 1991.