If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
You can find out where the majority of the Spicer families were living before they immigrated to the U.S and learn where to focus your search for foreign records. Immigration records can tell you an ancestor's name, ship name, port of departure, port of arrival, and destination. from the New York Passenger Lists.