If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Welcome to Spicewood Texas. Spicewood, Texas is a charming unincorporated town in Burnet County. It is home to Music Legend Willie Nelson. Even though it is not a big city, Spicewood has tremendous activities and impressive sites to see. A lot of individuals traveling to Spicewood come for the Lake Buchanan activities. Spicewood is home to Cypress Valley Canopy Tours, Pace Bend Park, Krause Springs, and Lakecliff Country Club.