Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
This time zone is called Mountain Standard Time (MST) during standard time (Winter). It is called Mountain Daylight Time (MDT) during daylight saving time (Summer). In the United States and Canada, this time zone is often just called Mountain Time (MT). The zone is one hour ahead of the Pacific Time Zone and one hour behind the Central Time Zone.