1 A do-it-yourself approach for basic basement steps starts around $250-$350 for materials, and takes about 3-4 hours. 2  That's with standard lumber, such as pine; costs can go much higher with more expensive wood, such as oak.
Pour enough melted agar into each sterile plastic petri dish to cover the bottom - about 1/8 to 1/4 deep. Replace the lid immediately. Place agar plates on a counter top to cool and set. Agar medium will set like stiff gelatin at room temperature. The agar medium is now ready for storage or use.
and gently swirling. It should be cool enough so that you can hold it in your hand for some time without it burning you. Or leave the melted agarose in a 50 Â°C waterbath. 8. Carefully pour the agarose solution into the gel mold and try to avoid bubbles. (Make sure the comb is in properly in place!) If you do get some bubbles, try to push them down towards the bottom edge of the gel with a tip. 9. Let the gel set completely and carefully pull up the comb. (submerge the gel in the gel tank makes it much easier to pull the comb out) 10. Put gel tray inside a gel box. Add enough 0.5XTBE buffer to cover the gel. (for large gel box, it needs 600-700 ml of buffer) 11.