If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Latimer is located at 42Â°45â²46â³N 93Â°22â²8â³W / 42.76278Â°N 93.36889Â°W / 42.76278; -93.36889 (42.762896, -93.368873). According to the United States Census Bureau, the city has a total area of 2.40 square miles (6.22 km2), all of it land.