If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
The Candler Ranch is a working cattle ranch and recreational ranch located just south of Hempstead on FM 1887, 45 minutes from downtown Houston. The ranch includes over 4,300 of highway frontage, two miles of Clear Creek frontage with plenty of wooded areas to complement the numerous pastures of improved grasses.