1 A do-it-yourself approach for basic basement steps starts around $250-$350 for materials, and takes about 3-4 hours. 2  That's with standard lumber, such as pine; costs can go much higher with more expensive wood, such as oak.
Pour enough melted agar into each sterile plastic petri dish to cover the bottom - about 1/8 to 1/4 deep. Replace the lid immediately. Place agar plates on a counter top to cool and set. Agar medium will set like stiff gelatin at room temperature. The agar medium is now ready for storage or use.
1 Hold the can upright (most blended coolants need to charge with the can upside down, in its liquid state. 2  This requires a different charging method.) and open the valve on your gauge. 3  Let the coolant flow for about 10 seconds then turn off the valve at the gauge, check you pressure. 4  Remember we are looking for 3-5 lbs.