If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
The surname Spice was first found in Devon where they held a family seat from very early times and were granted lands by Duke William of Normandy, their liege Lord, for their distinguished assistance at the Battle of Hastings in 1066 A.D. Conjecturally the Spicers were under tenants of the Count of Mortain.