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It is false that in an energy diagram for a chemical reaction, energy is plotted along the x-axis (the horizontal axis) and reaction progress is plotted along the y-axis (the vertical axis). The energy is actually plotted along the y-axis and the reaction along the x-axis.
Many experiments that suggest the possible sequence of steps in a reaction mechanism have been designed, including: 1  measurement of the effect of temperature (Arrhenius equation) to determine the activation energy. 2  spectroscopic observation of reaction intermediates.