If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Hancock Shaker Village is located on Route 20 in Pittsfield, Massachusetts, just west of the junction of Routes 20 and 41. 1  From Albany, New York (approximately 1 hour). 2  Take Route I-90 east to Exit 11E (Route 20) Nassau.