If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Located in Section 33 of Brookfield Township on the West side of Narrow Lake Road and southwest corner of East Butterfield Hwy overlooking Narrow Lake. It has been abandoned. Fox Cemetery : Located in section 10 of Brookfield Township on the south side of Spicerville Hwy, east of Narrow Lake Road.