Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
Eastern Time is 3 hours ahead of Pacific Time. The mainland U.S. has four time zones (Alaska and Hawaii each have their own time zones, which makes a total of 6 different timeâ¦ zones for the U.S.). From west to east, the mainland U.S.'s time zones are: Pacific Time, Mountain Time, Central Time and Eastern Time. Each is one hour later than the last. For example, if it's 3 PM Pacific Time, then it's 4 PM Mountain Time, 5 PM Central Time and 6 PM Eastern Time.