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The experiment aims to clarify the meaning of purity in the chemical sense ; apply the method of recrystallization in the purification of a solid sample; recognize melting point as a physical property that can serve as an index of purity; determine the melting point of a substance using a simple apparatus; and compare the melting point of a pure versus impure sample of a substance.
Melting curve analysis is an assessment of the dissociation-characteristics of double-stranded DNA during heating. As the temperature is raised, the double strand begins to dissociate leading to a rise in the absorbance intensity, hyperchromicity.he temperature at which 50% of DNA is denatured is known as the melting point, though it is an inaccurate term as it has very little to do with a traditional melting point. The information gathered can be used to infer the presence and identity of single-nucleotide polymorphisms (SNP).