Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
The U.S. has four main time zones. From west to east, they are: Pacific Time, Mountain Time, Central Time, and Eastern Time. E â¦ ach is one hour later than the last. For example, if it's 3 PM Pacific Time, then it's 4 PM Mountain Time, 5 PM Central Time, and 6 PM Eastern Time.Remember that going west always takes you back in time, and going east takes you into the future, so to speak.ach is one hour later than the last. For example, if it's 8 PM Pacific Time, then it's 9 PM Mountain Time, 10 PM Central Time, and 11 PM Eastern Time.