1 Yellow media without gas pocket-sugar utilization with production of acid only. 2  Red media (no change in media) -no change and no sugar utilization. 3  Pink media especially at the top, ignore gas-no sugar utilization with degradation of protein resulting in alkaline end products (this may be difficult to see).
With organic solvents with long alkane chain, such as n-hexadecane and n-decane, the water droplets become much larger and the microemulsion is unstable. In this case, solvent polarity may have become a determining factor and the long alkane chain was too hydrophobic to form a stable microemulsion with water.
n-Hexane is also used as a solvent in the extraction of oil from seeds (soybean, cottonseed, flaxseed, safflower seed, and others).