Coenzyme A (CoA, CoASH, or HSCoA) is a coenzyme, notable for its role in the synthesis and oxidation of fatty acids, and the oxidation of pyruvate in the citric acid cycle. All genomes sequenced to date encode enzymes that use coenzyme A as a substrate, and around 4% of cellular enzymes use it (or a thioester, such as acetyl-CoA) as a substrate. In humans, CoA biosynthesis requires cysteine, pantothenate, and adenosine triphosphate (ATP).[1]
TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat.#R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.he enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.
Polymerase chain reaction (PCR) is a technique used to exponentially amplify a specific target DNA sequence, allowing for the isolation, sequencing, or cloning of a single sequence among many.PCR was developed in 1983 by Kary Mullis, who received a Nobel Prize in chemistry in 1993 for his invention.ypically, a PCR is a three-step reaction. The sample containing a dilute concentration of template DNA is mixed with a heat-stable DNA polymerase, such as Taq polymerase, primers, deoxynucleoside triphosphates (dNTPs), and magnesium.