Roast the vegetables. Place the baking sheet with the vegetables into your preheated oven, on the middle or top rack. Roast for 15 minutes to an hour, depending on the vegetable. The vegetables are done when they are cooked through, the centers soft and creamy and the outer edges golden and crispy.
E. coli O157:H7 first became a household name during an outbreak in the western U.S. that began in late 1992, and lasted into 1993. The E. coli outbreak was linked to the consumption of hamburgers served by the Jack in the Box Restaurant chain.
The initial strain of E. coli for Lenski's long-term evolution experiment came from strain Bc251, as described in a 1966 paper by Seymour Lederberg, via Bruce Levin (who used it in a bacterial ecology experiment in 1972).