In general, yeasts are grown in the laboratory on solid growth media or in liquid broths. Common media used for the cultivation of yeasts include potato dextrose agar or potato dextrose broth, Wallerstein Laboratories nutrient agar, yeast peptone dextrose agar, and yeast mould agar or broth.
Defining Perforating. Connecting to the reservoir. Perforatingâthe act of blasting holes through steel casing, cement, and formation rockâlasts only an instant, but the long-term viability of most oil and gas assets depends upon it.
Trauma can also cause perforation. 1  Blunt or penetrating trauma, such as from a fall on the side of your head or a stick that goes deep in your ear. 2  Rapid changes in pressure, for example, scuba diving (barotrauma, ear pain, or ear squeeze), or going up in an elevator too fast.  The eardrum can be ruptured in other ways.