6 Generals Who Fought Against their Home State in the Civil War. For many Americans, choosing a side in the Civil War was no easy task. While most people simply sided with their home state, others struggled with family pressures, personal political beliefs and their devotion to the federal government.
An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells. For example, the epitope is the specific piece of the antigen to which an antibody binds. The part of an antibody that binds to the epitope is called a paratope. Although epitopes are usually non-self proteins, sequences derived from the host that can be recognized (as in the case of autoimmune diseases) are also epitopes.
When only a conjugated primary antibody is used for detection of the antigen. Epitope. The specific site where an antibody binds its antigen via its variable region. Note, this is not the same as the immunogen, which includes the epitope but is often much larger.his may help expose an epitope, or destroy it. Detection antibody. The primary antibody used in sandwich ELISA, which detects the immobilized antigen. This antibody can be directly conjugated or visualized following the application of a conjugated-secondary.