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Blood agar contains general nutrients and 5% sheep blood. It is useful for cultivating fastidious organisms and for determining the hemolytic capabilities of an organism. Some bacteria produce exoenzymes that lyse red blood cells and degrade hemoglobin; these are called hemolysins.
The agar pour method is considered the reference test procedure in Europe. The broth dilution method is more widely accepted in North America. The E test (AB Biodisk) is a plastic strip with a gradient concentration of antimicrobial agents impregnated in it.