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Abstract. Here we hypothesized that the water-soluble lysosomal enzymes may cross the bloodâbrain-barrier and reach the brain using the mechanism of unspecific fluid-phase endocytosis.n fact, there has been increasing evidence that when high serum levels of lysosomal enzymes are achieved, either by GT or ERT, a small fraction of the enzyme is able to cross the BBB by mechanisms not clearly understood [6] , [7] and [8] . Table 1.
The bloodâbrain barrier. (a) Whole-body autoradiogram of a mouse administered radiolabeled histamine intravenously [1]. The small molecule (i.e. 111 Da) enters all organs of the body, except for the brain and spinal cord. Histamine does not enter the CNS because histamine does not cross the bloodâbrain barrier (BBB). (b) India-ink study of the rat brain [52] shows the complexity of the cerebral microvasculature, which is the site of the BBB.