In this situation, it is a straightforward matter to cross one of the two strains to N2 males (or to generate a homozygous male stock from one of the mutant strains), and then cross the resultant heterozygous males to the second mutation to generate the a/+; b/+ trans-heterozygote.
Campbell County, Kentucky. Campbell County is a county located in the U.S. state of Kentucky. As of the 2010 census, the population was 90,336. Its county seats are Alexandria and Newport. The county was formed on December 17, 1794, from sections of Scott, Harrison, and Mason Counties and was named for Colonel John Campbell (1735â1799), a Revolutionary War soldier and Kentucky legislator.
Jamestown, KY. Jamestown is a city in Russell County, Kentucky, United States. The area was first settled in 1826 and named Jacksonville in honor of General Andrew Jackson. By 1827 the settlement was incorporated and renamed Jamestown in honor of James Wooldridge who had donated the land for the town. The population was 1,624 at the 2000 census. It is the county seat of Russell County. Jamestown is located at 36Â°59â²5â³N 85Â°4â²1â³W / 36.98472Â°N 85.06694Â°W / 36.98472; -85.06694 (36.984730, -85.066840).