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Abstract. Here we hypothesized that the water-soluble lysosomal enzymes may cross the bloodâbrain-barrier and reach the brain using the mechanism of unspecific fluid-phase endocytosis.n fact, there has been increasing evidence that when high serum levels of lysosomal enzymes are achieved, either by GT or ERT, a small fraction of the enzyme is able to cross the BBB by mechanisms not clearly understood [6] , [7] and [8] . Table 1.
The blood-brain barrier, which protects the central nervous system, is not an absolute barrier. 2,4-Dichlorophenoxyacetic acid (2,4-D), for example, has been measured in the rabbit brain after an iv dose (Kim et al., 1996).