In this situation, it is a straightforward matter to cross one of the two strains to N2 males (or to generate a homozygous male stock from one of the mutant strains), and then cross the resultant heterozygous males to the second mutation to generate the a/+; b/+ trans-heterozygote.
Campbell County, Kentucky. Campbell County is a county located in the U.S. state of Kentucky. As of the 2010 census, the population was 90,336. Its county seats are Alexandria and Newport. The county was formed on December 17, 1794, from sections of Scott, Harrison, and Mason Counties and was named for Colonel John Campbell (1735â1799), a Revolutionary War soldier and Kentucky legislator.
Pend Oreille County Historical Museum. Newport is a city in, and the county seat of, Pend Oreille County, Washington. The population was 2,126 at the 2010 census. Newport is part of the Spokane Metropolitan Area.