Calcium carbonate is also used in the purification of iron from iron ore in a blast furnace. The carbonate is calcined in situ to give calcium oxide, which forms a slag with various impurities present, and separates from the purified iron.
One re-hydrated package fills about 15-20 dishes. Mix 1 level teaspoon of dehydrated agar with 100 ml (3/8 cup) of water and then bring to a boil to make 100 ml of agar solution. Prepare Petri Dishes: Fill petri dishes to just cover the bottom. Typical 90-100mm dishes require 10-15 ml agar. Rotate the dish to obtain even surface coverage. Cover the dish immediately and let stand until firm. Store upside down in the refrigerator until ready to use.
Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 1  0.5% Peptone. 2  0.3% beef extract / yeast extract. 3  1.5% agar. 4  0.5% NaCl. 5  distilled water. 6  pH adjusted to neutral (6.8) at 25 Â°C.