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Blood agar contains general nutrients and 5% sheep blood. It is useful for cultivating fastidious organisms and for determining the hemolytic capabilities of an organism. Some bacteria produce exoenzymes that lyse red blood cells and degrade hemoglobin; these are called hemolysins.
Agar is the jelly-like substance used to culture bacteria. It is made from a type of red algae which provides an ideal growing surface for many different types of bacteria. Some types of agar contain added nutrients (such as sheep's blood) which help to promote more vigorous bacterial growth. In a heat proof dish or bowl, stir half a teaspoon of the nutrient agar powder into 60 ml (approximately 1/4 cup) of hot water. 2  Multiply these quantities for however many petri dishes you plan on using.