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The blastula is usually a spherical layer of cells (the blastoderm) surrounding a fluid-filled or yolk-filled cavity (the blastocoel). Mammals at this stage form a structure called the blastocyst, characterized by an inner cell mass that is distinct from the surrounding blastula.The blastocyst must not be confused with the blastula; even though they are similar in structure, their cells have different fates. Polar proliferation-Cells at the polar ends of the blastula/gastrula proliferate, mostly at the animal pole. 2  Other major changes during gastrulation: 3  Heavy RNA transcription using embryonic genes; up to this point the RNAs used were maternal (stored in the unfertilized egg).