In summary, we have shown the inhibitory effects of Bcl-2 family on the ceramide-mediated apoptotic pathway in glioma cells. Bcl-2 is considered to act not only downstream of ceramide to inhibit cytochrome c release followed by caspase activation, but also upstream of or at SMase to prevent ceramide generation.igure 3 Effects of Bcl-2 or Bax overexpression on ceramide formation and neutral sphingomyelinase activation in etoposide-treated C6 cells. C6/Neo (), C6/Bcl-2 (), and C6/Bax () cells were treated with 40 M etoposide for indicated periods.
Hawaii, which has the highest-paid waiters and waitresses in the country (mean wage: $13.91/hour) has a minimum wage of $7.00 for tipped employees.
This average pay rate is then used to calculate the overtime rate. Example: Jimmyâs restaurant has a waitress who makes $2.65 per hour as a waitress and $8 per hour as a hostess. She earned $200 in tips. common mistake is to simply take the $2.65 minimum tipped wages and multiply it by 1.5. Calculating Overtime When Employees Have Different Pay Rates. When employees have different pay rates during a pay period (e.g., a waitress also works as a hostess), a weighted average pay rate must be calculated.