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Using neutral red pH indicator, the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-)fermentation. This medium is also known as an indicator medium and a low selective medium. Presence of bile salts inhibits swarming by Proteus species.
View Full Document. lacP + lacO + lacZ + lacY + constitutively expresses the genes of the lac operon. The strain is converted to wild-type lac operon regulation by the addition of an extra piece of DNA. What gene or genes are contained on this extra DNA that explain this conversion to wild type; include an explanation of cis- or trans-acting factors and how they work.