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Using neutral red pH indicator, the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-)fermentation. This medium is also known as an indicator medium and a low selective medium. Presence of bile salts inhibits swarming by Proteus species.
In basic terms, lactic acid is the normal endpoint of the anaerobic breakdown of glucose in the tissues. The lactate exits the cells and is transported to the liver, where it is oxidized back to glucose.