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A cloture is a method for ending a debate and taking a vote. In the  U.S., invoking cloture requires a three-fifths vote of the entire  Senate.
The three-fifths version of the cloture rule does not apply to motions to end filibusters relating to Senate Rule changes. To invoke cloture to end debate over changing the Senate rules, the original version of the rule (two-thirds of those Senators present and voting) still applies. The procedure for invoking cloture, or ending a filibuster, is as follows: A minimum of 16 senators must sign a petition for cloture. The petition may be presented by interrupting another Senator's speech. The clerk reads the petition. The cloture petition is ignored for one full day during which the Senate is sitting (called a Legislative Day).