In summary, we have shown the inhibitory effects of Bcl-2 family on the ceramide-mediated apoptotic pathway in glioma cells. Bcl-2 is considered to act not only downstream of ceramide to inhibit cytochrome c release followed by caspase activation, but also upstream of or at SMase to prevent ceramide generation.igure 3 Effects of Bcl-2 or Bax overexpression on ceramide formation and neutral sphingomyelinase activation in etoposide-treated C6 cells. C6/Neo (), C6/Bcl-2 (), and C6/Bax () cells were treated with 40 M etoposide for indicated periods.
Hawaii, which has the highest-paid waiters and waitresses in the country (mean wage: $13.91/hour) has a minimum wage of $7.00 for tipped employees.
For example, assume a waitress in Florida makes $5.03 per hour. In one week, she works 40 hours and earns $80 in tips, bringing her total pay for the week to $281.20. When her total pay is divided by the number of hours she worked, her hourly rate only amounts to $7.03 â well below the Florida minimum wage of $8.05. Therefore, her employer is responsible for paying her a âminimum wage makeupâ of $40.80 on her paycheck; however, when she receives her paycheck, her employer did not pay her the additional âminimum wage makeup.â