The exact causes of chronic kidney failure are unknown, although the debilitating loss of healthy kidney tissue may be a natural endpoint for several readily recognizable conditions, including advanced dental disease and a variety of kidney infections, inflammations and obstructions.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
Report Abuse. In DNA extraction we use alcohol or isopropanol to concentrate DNA extract. what happens is that we use add sodium acetate or potassium acetate buffer (pH- 5.0) after the protein extraction step.sodium and acidic pH will neutralize the charged phosphate backbone and promote hydophobic interactions.est Answer: You add alcohol in order to concentrate the DNA and in order to get rid of the salts and other chemicals used in the extraction process.