Federal regulations permit the applicant/employee to request the split specimen that was collected at the time of the original specimen be sent to another laboratory for testing. This request should be made to the MRO within 72 hours of the employee being notified of the positive test result.
Shiga toxin-producing E. coli(STEC), also called verotoxinogenic E. coli, usually do not cause disease inanimals but may cause watery diarrhoea, haemorrhagic colitis, and/orhaemolytic uraemic syndrome in humans. Zoonotic STEC include the O157:H7strains and, with increasing frequency, certain non-O157 strains.
It preferentially binds double stranded DNA, but it can also bind single stranded DNA with reduced fluorescence. As with any DNA binding molecule, SYBR GreenÂ® can cause mutations and is a possible carcinogen. It is, however, safer to work with and easier to dispose of than ethidium bromide.