Preparation of MacConkey Agar. 1  Suspend the measured amount of powder (See in the agar bottle and generally 50 gram) in 1 L of purified water and mix thoroughly. 2  Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3  Autoclave at 121Â°C for 15 minutes.
All USDA loans (purchase and refinance) require a one-time Guarantee Fee that is added to the borrowers loan. The current amount of the USDA guarantee fee is 2%. Example â if a borrower was purchasing a home 100% financing for $150,000, the adjusted loan amount with the 2% USDA guarantee fee would be $153,000. $3,000 added to the base loan amount. In addition to this one time Guarantee Fee, USDA loans also require a annual fee that is added to the monthly mortgage payment. Basically the âannual feeâ is monthly mortgage insurance â or more commonly known as âPMIâ Again, like the above it can have many different names. The current amount of this fee is .5%.
USDA Announces Increase in Monthly Mortgage Insurance for 2015 Fiscal Year. The Annual Fee for USDA Section 502 Guaranteed Rural Housing loans will be increased from .40% to .50% for Fiscal Year 2015. Changes to the Annual Fee will be effective for all new GRH obligations as of October 1, 2014.The reason the change is happening in October is the USDA runs their fiscal year starting the last quarter of every year. EXAMPLE: On a $150,000 loan amount, the Annual Fee for 2013 is $600 per year, or $50 per month.tarting October 1st, that same loan amount would have an Annual Fee of $750, or $62.50 per month. This may not seem like a big change, but if you remember back before October 2011, the USDA did not charge an Annual Fee at all.